Jawad K. Abood Al-Janabi, currently Professor Emeritus of Mycology at the University of Babylon and Al-Mustaqbal University College, Iraq, has more than 30 years of experience in identification of fungi, biological control, host-parasite relationships and investigate the alternative technical approaches for defensive strategies of the individual pathogenic fungi.
11
Scopus Publications
361
Scholar Citations
10
Scholar h-index
11
Scholar i10-index
Scopus Publications
Characterization of newly recorded Pythium mamillatum AJA, causing cucumber root rot in Iraq: Implications for disease management in sustainable agriculture Jawad K. Abood Al-Janabi, Ali Nasir Hussein, Haider Jawad Al-Janabi, Ali R. Shakir Al-Shujairi Journal of Applied and Natural Science, 2025 Pythium mamillatum is an emerging pathogen responsible for root rot and wilt diseases in cucumber (Cucumis sativus), resulting in significant crop losses in Iraq. The present study examined the occurrence, distribution, pathogenicity, and genetic characterization of P. mamillatum strain AJA in Iraq, with broader implications for sustainable agricultural practices. Field surveys were conducted to collect symptomatic cucumber root samples from Al-Tahiria, Al-Azizia, and Al-Badah districts revealed disease incidences of 37%, 35%, and 21%, respectively. Infected plants exhibited symptoms of root rot and wilting. According to molecular research, the Iraqi strain, Pythium mamillatum AJA, accession No. MN460315.1 was 100% genetically related to strains from China, the USA, and Canada, indicating a shared evolutionary origin. Through inhibiting seed germination from 10% on the third day to 90% on the seventh day of inoculation, P. mamillatum (strain AJA) demonstrated significant losses to local Iraqi cucumber crops. The rapid spread of agriculture, its valuable genetic similarity with strains established in other regions worldwide, and its pathogenic viewpoint highlight its increasing threat. The study emphasizes the importance of employing effective disease control techniques within the framework of sustainable agriculture. Reducing the effect of this infection mostly depends on early identification, seed treatments, and constant pathogen monitoring. Maintaining crop health and guaranteeing long-term agricultural resilience also depend on sustainable farming methods that reduce the use of chemical pesticides and advance integrated pest management (IPM).
Identification and characterizations of a few species of Fusarium infecting cucumber in greenhouse conditions Ali Nasir Hussein, Haider Jawad. K. Al-Janabi, Jawad K. Abood Al-Janabi, Ali R. Shakir Al-Shujairi Journal of Applied and Natural Science, 2024 One of the most problematic and devastating diseases affecting cucumber production is Fusarium wilt (Cucumis sativus L.). The present study aimed to characterize Fusarium species isolated from three different regions of cucumber fields in Babylon province, Iraq and identify them based on morphological features and phylogenetic analyses. This study showed the presence of Fusarium incarnatum, F. solani and F. oxysporum in cucumber roots. Fusarium species were phylogenetically analyzed based on internal transcribed spacer (ITS) regions. All Fusarium isolates were pathogenic to cucumber cultivars but varied significantly in their growth and pathogenicity toward cucumber seeds during the experiment. F. solani-2 was greater in growth and pathogenicity than all other Fusarium species. In contrast, F. solani-1 was the lowest. The data obtained from morphological and molecular studies sufficiently supported each other, and the phylogenetic trees based on ITS were distinguished. Closely related species and distinctly separated all morphological taxa. These findings are reported for the first time for the cucumber plant in Babylon province. All these ITS sequences showed homologous to those of Fusarium species isolates in the GenBank database with a similarity percentage of 99%. To the best of present knowledge, this is the first molecular record of F. incarnatum on the cucumber plants in Iraq. The study concluded that F. incarnatum was reported for the first time in Iraq and worldwide as a causal agent of wilt disease in cucumber plants. The three species of Fusarium have different pathogenic abilities, highlighting their disease incidence, growth, and pathogenicity.
Antagonistic activities of bioagent fungi Trichoderma harzianum and Pleurotus ostreatus against three species of Fusarium in cucumber plants Nadir A. Hussein, Haider J. K. Al-Janabi, Fatimah R. Al-Mashhady, Jawad K. Abood Al-Janabi, Ali R. Shakir Al-Shujairi Asia Pacific Journal of Molecular Biology and Biotechnology, 2022 Many bioagent fungi have promising potential as eco-friendly alternatives to fungicides, with considerable antagonistic activity against various phytopathogenic fungi. The present study aimed to investigate the antagonistic activity of Trichoderma harzianum and Pleurotus ostreatus isolates against Fusarium spp., the causative agents of wilt disease in cucumber plants, through a dual plate assay of volatile and nonvolatile compounds from these bioagent fungi. The results showed significant (P < 0.05) antagonistic activities of T. harzianum against the growth of F. solani AJA2 (62.3%), followed by F. oxysporum AJA (55.2%), F. incarnatum AJA (53.2%), and F. solani AJA1 (50.8%). The effectiveness of P. ostreatus against the four Fusarium species was notably less than that of T. harzianum. In contrast, in the dual culture assay, the bioagent fungal filtrate exhibited inhibitory effects on the growth of all pathogens at 25% concentration. The highest inhibition rate (85%) was shown by T. harzianum against F. incarnatum. The percent of inhibition caused by P. ostreatus was substantially lower than that caused by T. harzianum, which reached 35% in F. incarnatum followed by other pathogens. The volatile compounds of T. harzianum led to a high percentage of inhibition of all the three Fusarium species, while the highest percentage of inhibition due to the compounds of P. ostreatus was observed only for F. solani AJA1 (41.5%). From these results, we concluded that despite the diverse inhibitory effects of both bioagent fungi against Fusarium species, they exhibited successful antagonistic activity and the ability to compete against these species.
Detection of genetic polymorphisms using random amplified polymorphic DNA (RAPD)-PCR in fenugreek (trigonella foenum-graecum) plants after seed treatment with biotic and abiotic agents Zahraa Abid Nima Al-Yasiri, Basheer Al-Alwani, Jawad K. Abood Al-Janabi Journal of Pure and Applied Microbiology, 2021 Trigonella foenum-graecum L. is a widely used herb in traditional medicine. The aim of this study was to evaluate the genetic polymorphisms in fenugreek plants following the treatment of fenugreek seeds with different combinations of biotic and abiotic agents using the random amplified polymorphic DNA (RAPD)-PCR technique. We assessed the effects of two strains of the fungus Trichoderma harzianum (Th-1 and Th-2), methyl jasmonate (MeJA), and Aloe vera gel (AVG) on growth parameters of fenugreek plants. Combinations of Th-1, MeJA, AVG significantly increased fenugreek root length, shoot length, shoot fresh weight, number of true leaves, and chlorophyll content. The Th-2 isolate, on the other hand, markedly slowed plant development (except for root length which was not affected significantly). In contrast, the combination with MeJA had no considerable effect on all growth measures, whereas the combination with VAG resulted in a substantial drop in shoot height and chlorophyll content when compared to other growth parameters that were unaffected. The present study has shown that the PCR amplification of DNA, using five primers for RAPD analysis, produced 62 DNA fragments that could be scored in all genotypes. The total number of polymorphic bands was 26, and the average percentage of polymorphism was 54.21%. The RAPD-PCR results showed that the treatment of fenugreek seeds with Th-1 alone or in combination with MeJA and AVG induced polymorphisms in fenugreek leaves.
Bioactivities of anethole, astragalin and cryptochlorogenic acid extracted from anise oil and moringa oleifera on the keratinase gene expression of trichophyton rubrum Ali Jalil Obaid, , Jawad K. Abood Al-Janabi, Wijdan Ridha Taj-Aldin, , and Journal of Pure and Applied Microbiology, 2020 Trichophyton rubrum is the most common cause of dermatophyte skin infections in humans worldwide. The aim of the current study is to search for effective and safe antifungal agents by investigating the bioactivity of Pimpinella anisum and Moringa oleifera extracts, including the impact on the keratinase gene expression of T. rubrum. Astragalin and cryptochlorogenic acid were detected in M. oleifera leaf extracts by Gas chromatography-mass spectrometry (GC-MS), whereas anethole was extracted from P. anisum oil. The expression of the keratinase gene was assessed in two strains of T. rubrum (KP979791 and KP979787) in Sabouraud Dextrose Broth (SDB) medium (containing 10% keratin) separately supplemented with plant extracts fractions. The results showed that cryptochlorogenic acid and astragalin were active compounds (molecular weights of 353.26 and 449.35 g/mol, respectively that considerably down-regulated the keratinase genes in both strains of T. rubrum. M. oleifera crude extract, astragalin, cryptochlorogenic acid, anise oil, and anethole resulted in the down-regulation of the keratinase genes of T. rubrum strain KP979791 by 0.048, 0.291, 0.001, 0.007, and 0.009 (foldchange), respectively, and that of strain KP979787 by 0.151, 0.42, 0.16, 0.079, and 0.092 as compared to the control (1.00). Nearly all of these outcomes are novel findings that may aid in the identification of novel effective drug targets and antifungal agents.
Alternative culture media for growth and sporulation of trichodermaharzianum Pakistan Journal of Biotechnology, 2017
Changes in carbohydrate composition of cucumber leaves during the development of powdery mildew infection J. K. Abood, D. M. Losel Plant Pathology, 2003 Within 3 days after inoculation, greater rates of decrease of hexoses, and of increases in inositol and α-glucans, were detected in leaves of cucumber leaves infected with Sphaerotheca fuliginea (now reclassified as Podosphaera xanthii) than in healthy leaves, together with synthesis of fungal mannitol, arabitol and trehalose. With the onset of sporulation (5 days after inoculation, dai), glucose, polyols, trehalose and glycogen content, as well as fresh and dry weight of leaf tissue, increased more rapidly than in healthy plants, while sucrose, raffinose and starch declined. Starch, glycogen and other α-glucans, extracted from ethanol-insoluble tissue fractions of different water-solubility from healthy and infected leaves, were quantified after amyloglucosidase treatment. Infection-induced alterations in partitioning of photosynthate during a 14 h photoperiod at 7 dai, including a close correspondence in patterns of synthesis of glucose, polyols and glycogen, further clarified the process of diversion of host assimilate to the pathogen. The presence of glucose, but only traces of sucrose in spores, supported evidence from other powdery mildew infections for photosynthate being transferred to S. fuliginea as glucose.
Role of Membrane Lipids in Powdery Mildew Infection of Cucumber Dorothy M. Lösel, Emily T. Meilleur, Jawad K. Abood Journal of Plant Physiology, 1994 Summary Since altered partitioning of photosynthate into lipids of cucumber leaf tissue, particularly increased synthesis of PI, during infection by Sphaerotheca fuliginea , the reversal of such changes in the presence of lithium chloride, pointed to the possible stimulation of the inositol phosphatide cycle in the hostpathogen interaction, further evidence was sought in this limited study of myo-[2- 3 H]inositol incorporation by healthy and infected leaves. Compared with healthy tissue, infected leaf tissue showed increased labelling of inositol phosphatides IP 1–3 , during a 36h chase period. This finding is consistent with the hypothesis that the reduction in powdery mildew disease, observed in cucumber plants supplied systemically with lithium chloride (Abood et al., 1991) could be due to interference with phosphatidyl inositol turnover.
Changes in abundance and infectivity of powdery mildew conidia from cucumber plants treated systemically with lithium chloride J. K. ABOOD, D. M. LÖSEL, P. G. AYRES Plant Pathology, 1992 Systemic treatment of cucumber plants with lithium chloride reduced the numbers of conidia produced by colonies of powdery mildew, Sphaerotheca fuliginea, growing on leaves, and lowered the infectivity of conidia produced from those leaves when they were applied to leaves of untreated plants. Production of conidiophores was lower in both lithium-treated and calcium-deprived plants, and lithium slightly decreased the calcium content of leaves. When the lithium-containing growth medium was supplemented with phosphate, conidiophore production was still markedly reduced, although leaves had normal levels of calcium. Fungal development was not correlated with either the calcium or phosphorus content of leaves. It is concluded that, although severe calcium deficiency can inhibit fungal development, the inhibitory effects of lithium are not mediated through alterations in calcium or phosphorus uptake by host tissues.
Characterization of newly recorded Pythium mamillatum AJA, causing cucumber root rot in Iraq: Implications for disease management in sustainable agriculture JKA Al-Janabi, AN Hussein, HJ Al-Janabi, ARS Al-Shujairi Journal of Applied and Natural Science 17 (3), 1325 , 2025 2025 Citations: 1
Identification and characterizations of a few species of Fusarium infecting cucumber in greenhouse conditions AN Hussein, HJK Al-Janabi, JKA Al-Janabi, ARS Al-Shujairi Journal of Applied and Natural Science 16 (1), 209 , 2024 2024 Citations: 4
Antagonistic activities of bioagent fungi Trichoderma harzianum and Pleurotus ostreatus against three species of Fusarium in cucumber plants NA Husseina, HJ Al-Janabib, FR Al-Mashhadyc, JKA Al-Janabia, ... Asia Pac. J. Mol. Biol. Biotechnol 30 (1), 12-21 , 2022 2022 Citations: 8
A novel Screening Methods using SSCP –PCR to Detect Variations Among three Varieties of Trigonella Foenum Graecum L. Collected from Different Regions in Iraq BAA Zahraa Abid Nima Al-Yasiri, Jawad K. Abood Al-Janabi Indian Journal of Forensic Medicine & Toxicology 15 (3), 5200-5205 , 2021 2021
Detection of Genetic Polymorphisms using Random Amplified Polymorphic DNA (RAPD)-PCR in Fenugreek (Trigonella foenum-graecum) Plants after Seed Treatment with Biotic and … JKAAJ Zahraa Abid Nima Al-Yasiri, Basheer Al-A Journal of Pure and Applied Microbiology 15 , 2021 2021 Citations: 6
Occurrence, characterizations of ochratoxin a and molecular identification of Aspergillus ochraceous contaminating dried fruits A Hadi, IM Al Hussaini, JKA Al-Janabi Annals of the Romanian Society for Cell Biology 25 (4), 13811-13826 , 2021 2021 Citations: 4
Molecular characterization and gene expression profiling of Trichophyton rubrum treated with a Marasmius palmivorus filtrate NNH Al-Masaoodi, JKA Al-Janabi, BT Mohammed Drug Invention Today 14 (6), 877-888 , 2020 2020 Citations: 8
Occurrence, morphological, and molecular characteristics of Trichophyton erinacei in Iraq NNHAL Masaoodi, BT Mohammed, JKA Al-Janabi Drug Invention Today 14 (6), 889-896 , 2020 2020 Citations: 6
Bioactivities of Anethole, Astragalin and Cryptochlorogenic Acid Extracted from Anise Oil and Moringa oleifera on the Keratinase Gene Expression of Trichophyton rubrum AJ Obaid, JKA Al-Janabi, WR Taj-Aldin Journal of Pure and Applied Microbiology 14 (1), 615-626 , 2020 2020 Citations: 11
Efficiency of antifungal agents with culture filtrates of two basidiomycetes fungi on growth and on citrate and isocitrate genes of Trichophyton rubrum MH Abdullah, JKA Al-Janabi Drug Invention Today 11 (11) , 2019 2019 Citations: 5
Antifungal activity of combination of medicinal plant extracts with terbinafine through regulating subtilisin virulence genes in Microsporum canis MQH Khazia, JKA Al-Janabi Drug Invention Today 11 (11) , 2019 2019 Citations: 9
Physiological Studies on Growth and Reproduction of Sclerotinia sclerotiorum Causing White Rot on Eggplants under Laboratories Conditions AHH Radhi, JK Al–Janabi Journal of University of Babylon for Pure and Applied Sciences 27 (4), 205-222 , 2019 2019
Characterization and Antagonistic Activity of New Causal Agent of Wilt Disease in Imperata cylindrica (Marasmius palmivorus) A tamur, HJ Al-Janabi, JKA Al-Janabi, ZAN Al-Yassiry Journal of Pure and Applied Microbiology 13 (3), 1525-1536 , 2019 2019 Citations: 11
ALTERNATIVE CULTURE MEDIA FOR GROWTH AND SPORULATION OF TRICHODERMA HARZIANUM AT Hassan, YM Liqaa, KAAJ Jawad, ANAY Zahraa Pakistan Journal of Biotechnology 15 (1), 29-35 , 2018 2018
MARASMIELLUS PALMIVORUS AS A NEW CAUSAL AGENT OF REED WILT DISEASE IN IRAQ AT Hassan, YM Liqaa, KAAJ Jawad, ANAY Zahraa Pakistan Journal of Biotechnology 15 (1), 29-35 , 2018 2018 Citations: 10
Phenotypic and Molecular Identification of Trichophyton Rubrum and Microsporum gypseum of Dermatophytosis FAHA Ali, JKA Al-Janabi Journal of Global Pharma Technology 10 (9), 103-111 , 2017 2017 Citations: 6
ALTERNATIVE CULTURE MEDIA FOR GROWTH AND SPORULATION OF TRICHODERMAHARZIANUM. LY Mohsen, HJ Kadhim, JKA Al-Janabi, AN Zahraa Pakistan Journal of Biotechnology 14 (4) , 2017 2017 Citations: 6
Chemical Composition and Bioactivity Characteristics of Pimpinella Anisum Essential Oil against Trichophyton rubrum AJ Obaid, JKA Al-Janabi, WR Taj-Aldin Journal of Global Pharma Technology 8 (9), 44-56 , 2017 2017 Citations: 16
The expression of resistance genes in tomato induced by abiotic and biotic factors against Fusarium oxysporum f. sp. Lycopersici AA Alnuaimy, JKA AL-Janabi, LAH ALObaidi, AF Marjan International Journal of ChemTech Research 10 (6), 838-850 , 2017 2017 Citations: 7
Prevalence of dermatophyte fungal infection in Hillah, Iraq MKA Fatima Alzahraa H. Abed Ali , Jawad K. Abood Al-Janabi International Journal of ChemTech Research 10 (6), 827-837 , 2017 2017 Citations: 21
MOST CITED SCHOLAR PUBLICATIONS
Changes in carbohydrate composition of cucumber leaves during the development of powdery mildew infection JK Abood, DM Lösel Plant pathology 52 (2), 256-265 , 2003 2003 Citations: 66
In vitro, determination of optimal conditions of growth and proteolytic activity of clinical isolates of Trichophyton rubrum SK Kadhim, JK Al-Janabi, AH Al-Hamadani Journal of contemporary medical sciences 1 (3), 9-19 , 2015 2015 Citations: 34
The growth and production of patulin mycotoxin by Penecillium expansum on apple fruits and its control by the use of propionic acid and sodium benzoate. L Larous, N Hendel, JK Abood, M Ghoul 2007 Citations: 31
Prevalence of dermatophyte fungal infection in Hillah, Iraq MKA Fatima Alzahraa H. Abed Ali , Jawad K. Abood Al-Janabi International Journal of ChemTech Research 10 (6), 827-837 , 2017 2017 Citations: 21
Chemical Composition and Bioactivity Characteristics of Pimpinella Anisum Essential Oil against Trichophyton rubrum AJ Obaid, JKA Al-Janabi, WR Taj-Aldin Journal of Global Pharma Technology 8 (9), 44-56 , 2017 2017 Citations: 16
The effect of some environmental conditions on the growth and activity of the external enzymes for five sp. of Fusarium L Mohsen, JK Al-Janabi, MA Jebor J. Babylon Univ./Pure Appl. Sci 3, 630-646 , 2016 2016 Citations: 15
Antifungal Activity of Anise Essential Oil against Growth and Morphological Characteristics of Trichophyton Rubrum WRTA Ali Jalil Obaid, Jawad K. Abood Al-Janabi Journal of Global Pharma Technology 7 (9), 53-68 , 2017 2017 Citations: 12
Bioactivities of Anethole, Astragalin and Cryptochlorogenic Acid Extracted from Anise Oil and Moringa oleifera on the Keratinase Gene Expression of Trichophyton rubrum AJ Obaid, JKA Al-Janabi, WR Taj-Aldin Journal of Pure and Applied Microbiology 14 (1), 615-626 , 2020 2020 Citations: 11
Characterization and Antagonistic Activity of New Causal Agent of Wilt Disease in Imperata cylindrica (Marasmius palmivorus) A tamur, HJ Al-Janabi, JKA Al-Janabi, ZAN Al-Yassiry Journal of Pure and Applied Microbiology 13 (3), 1525-1536 , 2019 2019 Citations: 11
MARASMIELLUS PALMIVORUS AS A NEW CAUSAL AGENT OF REED WILT DISEASE IN IRAQ AT Hassan, YM Liqaa, KAAJ Jawad, ANAY Zahraa Pakistan Journal of Biotechnology 15 (1), 29-35 , 2018 2018 Citations: 10
Antifungal activity of combination of medicinal plant extracts with terbinafine through regulating subtilisin virulence genes in Microsporum canis MQH Khazia, JKA Al-Janabi Drug Invention Today 11 (11) , 2019 2019 Citations: 9
Antagonistic activities of bioagent fungi Trichoderma harzianum and Pleurotus ostreatus against three species of Fusarium in cucumber plants NA Husseina, HJ Al-Janabib, FR Al-Mashhadyc, JKA Al-Janabia, ... Asia Pac. J. Mol. Biol. Biotechnol 30 (1), 12-21 , 2022 2022 Citations: 8
Molecular characterization and gene expression profiling of Trichophyton rubrum treated with a Marasmius palmivorus filtrate NNH Al-Masaoodi, JKA Al-Janabi, BT Mohammed Drug Invention Today 14 (6), 877-888 , 2020 2020 Citations: 8
Changes in abundance and infectivity of powdery mildew conidia from cucumber plants treated systemically with lithium chloride JK Abood, DM Lösel, PG Ayres Plant pathology 41 (3), 255-261 , 1992 1992 Citations: 8
The expression of resistance genes in tomato induced by abiotic and biotic factors against Fusarium oxysporum f. sp. Lycopersici AA Alnuaimy, JKA AL-Janabi, LAH ALObaidi, AF Marjan International Journal of ChemTech Research 10 (6), 838-850 , 2017 2017 Citations: 7
Production and Characterization of Exopolygalacturonase for Fusarium oxysporum and F. sacchari LY Mohsen, JKA Al-Janabi, MA Jebor International Journal of Science and Research (IJSR) 5 (1), 1315-1321 , 2016 2016 Citations: 7
Genotyping and subgenotyping of Trichophyton rubrum isolated from dermatophytosis in Iraqi patients using RFLP-PCR Sara k. Kadhim, Adnan H. Al-Hamadani, Jawad k. Al-Janabi IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) 10 (6), 61-67 , 2015 2015 Citations: 7
Detection of Genetic Polymorphisms using Random Amplified Polymorphic DNA (RAPD)-PCR in Fenugreek (Trigonella foenum-graecum) Plants after Seed Treatment with Biotic and … JKAAJ Zahraa Abid Nima Al-Yasiri, Basheer Al-A Journal of Pure and Applied Microbiology 15 , 2021 2021 Citations: 6
Occurrence, morphological, and molecular characteristics of Trichophyton erinacei in Iraq NNHAL Masaoodi, BT Mohammed, JKA Al-Janabi Drug Invention Today 14 (6), 889-896 , 2020 2020 Citations: 6