Shagufta Perveen
@bact.wisc.edu
Scientist III at Department of Bacteriology
University of Wisconsin - Madison
Dr. Shagufta Perveen, a Pakistani national, Natural Product Chemist/Scientist by profession, born in a religious, modern, well-educated and patriotic family. Her father, Muhammad Kamran Khan is Deputy Manager (R) from civil engineering department of Pakistan Steels Mills Corporation and mother Razia Khanum is a housewife. Since Pakistan came into being, most of her family members were serving Government of Pakistan in different departments such as Pakistan Railway, Accountant General Office, Education, Police, etc.
Dr. Shagufta obtained her early education from her home town Shah Faisal Colony, Karachi and later moved to University of Karachi and graduated in Organic Chemistry with 1st division. After graduation, she got scholarship from Higher Education Commission of Pakistan and joined one of the finest institutes of Pakistan, International Center for Chemical and Biological Sciences (HEJ Research Institute of Chemistry and Dr Panjwani Center for Molecular Medicine and Drug Research), University of Karachi for higher studies in Chemistry and Biology. The ICCBS was governed by world’s well-known scientists including Professor Dr Atta-ur-Rahman a UNESCO, Science Laureate (F.R.S., N.I., H.I., S.I., T.I.) as Patron-in-Chief and his mentee Prof. Dr Muhammad Iqbal Choudhary (H.I., S.I., T.I.) as Director. Dr Shagufta started her PhD in 2003 under supervision of Prof Dr Abdul Malik (R) (S.I.), who was a senior faculty member of ICCBS and also performed duties of acting director in the absence of Director. Dr Shagufta completed Part A of her thesis topic “Phytochemical Investigation on the Chemical Constituents of Perovskia Atriplicifolia” from ICCBS (HEJ), University of Karachi, Pakistan.
At the end of 2006, she got another short-term scholarship and joined Prof. Dr Jeremy D Kilburn (Head of School of Chemistry) at University of Southampton, GB, where she completed Part B of her thesis topic “The Synthesis of Super-molecules”.
EDUCATION
1. 2009 PhD (Natural Product Chemistry / Organic Chemistry / Synthetic Chemistry):
Thesis Part A from the HEJ Institute of Chemistry, University of Karachi, Karachi, Pakistan; under supervision of Professor Dr Abdul Malik, topic "Phytochemical Investigation on the Chemical Constituents of Perovskia Atriplicifolia".
Thesis Part B from the School of Chemistry, the University of Southampton, Southampton, UK; under supervision of Professor Jeremy D Kilburn (Head of School), topic "The Synthesis of Supramolecules".
2. 2002 MSc (Organic Chemistry) from University of Karachi, Karachi, Pakistan.
3. 1998 BSc (Chemistry/Biology/Physics) from University of Karachi, Karachi, Pakistan.
RESEARCH, TEACHING, or OTHER INTERESTS
Multidisciplinary
FUTURE PROJECTS
Beauty and Anti-Bacterial Soap
Applications Invited
Testing & Patent
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Shagufta Perveen, Amna Shoaib, Hafiza Sibgha Yaqoob, Ghanwa Riaz, and Muhammad Rafiq
Elsevier BV
Fakhira Bashir, Aqsa Afzaal, Shahnaz, Mazhar Amjad Gilani, Muhammad Saleem, Shagufta Perveen, Ahmad Junaid Ashraf, Olivier Huck, and Sobia Tabassum
Elsevier BV
Eman Fikry, Raha Orfali, Nora Tawfeek, Shagufta Perveen, Safina Ghafar, Maher M. El-Domiaty, and Azza M. El-Shafae
MDPI AG
Prior studies have extensively investigated the essential oil derived from the Mediterranean cypress, Cupressus sempervirens. However, the ‘Stricta’ variety, known for its ornamental value, has received less attention in terms of its oil composition and potential health benefits. The objective of this research was to comprehensively analyze the chemical components and medicinal properties of the essential oil extracted from C. sempervirens ‘Stricta’ (CSSLEO) grown in Egypt. Utilizing gas chromatography–mass spectrometry (GC–MS), the investigation identified 22 compounds within CSSLEO, with α-pinene and δ-3-carene being predominant, accounting for 96.01% of the oil. In vitro assays evaluated CSSLEO’s cytotoxic effects on cancer cell lines, revealing notable anticancer potential. Additionally, the oil displayed antidiabetic properties by impeding crucial enzymes involved in glucose metabolism. Complementary in silico network pharmacology and molecular docking studies provided insights into the possible interactions between CSSLEO’s key compounds and essential proteins and pathways in cancer treatment. The results underscored CSSLEO’s intricate composition and its promising applications in cancer prevention and diabetes management. The conclusions drawn from this research underscore the need for further investigation to validate CSSLEO’s clinical effectiveness and to gain a deeper understanding of its therapeutic mechanisms, with a view to harnessing its potential in oncology and endocrinology.
Safina Ghaffar, Rizwana Sanaullah Waraich, Raha Orfali, Areej Al-Taweel, Hanan Y. Aati, Sonia Kamran, and Shagufta Perveen
MDPI AG
The current study intended to investigate the role of new natural compounds derived from the Sesuvium sesuvioides plant in mitigating symptoms of diabetes and insulin resistance in the diabetic mice model. Anti-advanced glycation activity, insulin, and adiponectin were quantified by enzyme-linked immunosorbent assay (ELISA). Glucose uptake was performed using enzymatic fluorescence assay, and glycogen synthesis was measured using PAS staining. Gene and protein expression was assessed using real time PCR (RT-PCR), and immunoblotting and fluorescent microscopy, respectively. The new flavonoid glycoside eupalitin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside 1 isolated from S. sesuvioides exhibited anti-AGE activity by reducing human glycated albumin in liver cells. In a diabetic mouse model treated with compound 1, we observed improved glucose tolerance, increased adiponectin levels, and decreased insulin resistance. We also observed alleviated AGEs induced reduction in glucose uptake and restored glycogen synthesis in the compound 1-treated diabetic mice muscles. Exploring the molecular mechanism of action in skeletal muscle tissue of diabetic mice, we found that 1 reduced AGE-induced reactive oxygen species and the inflammatory gene in the muscle of diabetic mice. Additionally, 1 exhibited these effects by reducing the gene and protein expression of receptor for advanced glycation end products (RAGE) and inhibiting protein kinase C (PKC) delta activation. This further led us to demonstrate that compound 1 reduced serine phosphorylation of IRS-1, thereby restoring insulin sensitivity. We conclude that a new flavonoid glycoside from S. sesuvioides could be a therapeutic target for the treatment of symptoms of insulin resistance and diabetes.
Moiz U din Khan, Aqsa Afzaal, Shahnaz, Mazhar Amjad Gilani, Shagufta Perveen, Faiza Sharif, Anila Asif, Abrar Faisal, Muhammad Shahid Nazir, Olivier Huck,et al.
IOP Publishing
Abstract Skeletal defects are the second-leading cause of disability worldwide, prompting the development of smart solutions for treatment. Calcium glycerophosphate (Ca–GP), chitosan (CS), hydroxyapatite (HA), and zein (ZN) were used to fabricate these thermo-responsive hydrogels. Ca–GP, an economically viable and bioactive glycerophosphate source, remains relatively underexplored. Natural protein ZN and the gold standard bone regenerative biomaterial HA were incorporated as reinforcing agents. The resulting composite hydrogels (HGs) exhibit a sol phase at 4 °C–10 °C and transition to gels at body temperature within 4–6 min. Their good injectability and the ability to be easily shaped into complex structures further support their great potential as minimally invasive solutions for treatment. The addition of ZN significantly improved the mechanical and biological properties of the HGs. The highest ZN concentration resulted in the strongest mechanical strength, measuring 52.2 MPa at 40% strain. HGs exhibited optimal swelling and degradation rates. Scanning electron microscopy analysis supported their porous nature. In vitro cell culture assays and wound healing assays demonstrated their excellent biocompatibility and regenerative potential. Drug-loaded HGs exhibited up to 90% drug release and antibacterial activity. All these results support their promising potential to support the regeneration of skeletal defects in a minimally invasive manner.
Muhammad Rafiq, Amna Shoaib, Arshad Javaid, Shagufta Perveen, Muhammd Umer, Muhammad Arif, and Chunsong Cheng
Elsevier BV
Raha Orfali, Safina Ghaffar, Lateefa AlAjlan, Shagufta Perveen, Eman Al-Turki, and Fuad Ameen
Elsevier BV
Fredi Cifuentes, Javier Palacios, Daniel Asunción-Alvarez, Ricardo D. G. de Albuquerque, Mario J. Simirgiotis, Adrián Paredes, Chukwuemeka R. Nwokocha, Raha Orfali, and Shagufta Perveen
Springer Science and Business Media LLC
Fredi Cifuentes, Javier Palacios, Daniel Asunción-Alvarez, Ricardo D. G. de Albuquerque, Mario J. Simirgiotis, Adrián Paredes, Chukwuemeka R. Nwokocha, Raha Orfali, and Shagufta Perveen
Springer Science and Business Media LLC
Ghanwa Riaz, Amna Shoaib, Sidra Javed, Shagufta Perveen, Waseem Ahmed, Mohamed A. El-Sheikh, and Prashant Kaushik
Elsevier BV
Shagufta Perveen, Azadeh Hamedi, Ardalan Pasdaran, Reza Heidari, Muhammad Shafiq ul Azam, Sobia Tabassum, Rashad Mehmood, and Jiangnan Peng
Springer Science and Business Media LLC
Muhammad Atif and Shagufta Perveen
Springer Science and Business Media LLC
Muhammad Hamzah Saleem, Abida Parveen, Shagufta Perveen, Naheed Akhtar, Fozia Abasi, Maria Ehsan, Habib Ali, Mohammad K. Okla, Ibrahim A. Saleh, Naser Zomot,et al.
Springer Science and Business Media LLC
Muhammad Hamzah Saleem, Abida Parveen, Shagufta Perveen, Naheed Akhtar, Fozia Abasi, Maria Ehsan, Habib Ali, Mohammad K. Okla, Ibrahim A. Saleh, Naser Zomot,et al.
Springer Science and Business Media LLC
Hafiza Sibgha Yaqoob, Amna Shoaib, Aneela Anwar, Shagufta Perveen, Sidra Javed, and Samina Mehnaz
Springer Science and Business Media LLC
Muhammad Saeed, Muhammad Naeem, Alia Javed, Shagufta Perveen, Iqra Sajjad, Muhammad Zaid Yousaf, M. Shahid Munir Chohan, Muhammad Riaz, Sana Ullah, and Xianliang Song
Springer Science and Business Media LLC
Eman Fikry, Raha Orfali, Shaimaa S. El-Sayed, Shagufta Perveen, Safina Ghafar, Azza M. El-Shafae, Maher M. El-Domiaty, and Nora Tawfeek
MDPI AG
Methotrexate (MTX) therapy encounters significant limitations due to the significant concern of drug-induced liver injury (DILI), which poses a significant challenge to its usage. To mitigate the deleterious effects of MTX on hepatic function, researchers have explored plant sources to discover potential hepatoprotective agents. This study investigated the hepatoprotective effects of the ethanolic extract derived from the aerial parts of Chamaecyparis lawsoniana (CLAE) against DILI, specifically focusing on MTX-induced hepatotoxicity. UPLC-ESI-MS/MS was used to identify 61 compounds in CLAE, with 31 potential bioactive compounds determined through pharmacokinetic analysis. Network pharmacology analysis revealed 195 potential DILI targets for the bioactive compounds, including TP53, IL6, TNF, HSP90AA1, EGFR, IL1B, BCL2, and CASP3 as top targets. In vivo experiments conducted on rats with acute MTX-hepatotoxicity revealed that administering CLAE orally at 200 and 400 mg/kg/day for ten days dose-dependently improved liver function, attenuated hepatic oxidative stress, inflammation, and apoptosis, and reversed the disarrayed hepatic histological features induced by MTX. In general, the findings of the present study provide evidence in favor of the hepatoprotective capabilities of CLAE in DILI, thereby justifying the need for additional preclinical and clinical investigations.
Mohammad K. Okla, Muhammad Hamzah Saleem, Ibrahim A. Saleh, Naser Zomot, Shagufta Perveen, Abida Parveen, Fozia Abasi, Habib Ali, Baber Ali, Yasmeen A. Alwasel,et al.
Springer Science and Business Media LLC
AbstractIn the current industrial scenario, cadmium (Cd) as a metal is of great importance but poses a major threat to the ecosystem. However, the role of micronutrient − amino chelates such as iron − lysine (Fe − lys) in reducing Cr toxicity in crop plants was recently introduced. In the current experiment, the exogenous applications of Fe − lys i.e., 0 and10 mg L − 1, were examined, using an in vivo approach that involved plant growth and biomass, photosynthetic pigments, oxidative stress indicators and antioxidant response, sugar and osmolytes under the soil contaminated with varying levels of Cd i.e., 0, 50 and 100 µM using two different varieties of canola i.e., Sarbaz and Pea − 09. Results revealed that the increasing levels of Cd in the soil decreased plant growth and growth-related attributes and photosynthetic apparatus and also the soluble protein and soluble sugar. In contrast, the addition of different levels of Cd in the soil significantly increased the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), which induced oxidative damage in both varieties of canola i.e., Sarbaz and Pea − 09. However, canola plants increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and non-enzymatic compounds such as phenolic, flavonoid, proline, and anthocyanin, which scavenge the over-production of reactive oxygen species (ROS). Cd toxicity can be overcome by the supplementation of Fe − lys, which significantly increased plant growth and biomass, improved photosynthetic machinery and sugar contents, and increased the activities of different antioxidative enzymes, even in the plants grown under different levels of Cd in the soil. Research findings, therefore, suggested that the Fe − lys application can ameliorate Cd toxicity in canola and result in improved plant growth and composition under metal stress.
Muhammad Atif, Shagufta Perveen, Abida Parveen, Sara Zafar, Fatima Batool, and Farah Saeed
Springer Science and Business Media LLC
Khalid Sultan, Shagufta Perveen, Abida Parveen, Muhammad Atif, and Sara Zafar
Springer Science and Business Media LLC
Sidra Hameed, Muhammad Atif, and Shagufta Perveen
Springer Science and Business Media LLC
Khlood Jamal, Areej Al-Taweel, Sarah I. Bukhari, Raha Orfali, Nadine M. S. Moubayed, Jawaher Al-Qahtani, Hanan Aati, Orazio Taglialatela-Scafati, Jiangnan Peng, and Shagufta Perveen
MDPI AG
A phytochemical investigation of the stems of the Arabian plant Artemisia sieberi afforded three new isochlorogenic acid derivatives, namely isochlorogenic acid A-3′-O-β-glucopyranoside (1), isochlorogenic acid A-3′-O-β-glucopyranoside methyl ester (2), and isochlorogenic acid C-3′-O-β-glucopyranoside (3), obtained along with thirteen known secondary metabolites belonging to distinct structural classes. The structures of the new metabolites were elucidated by modern spectroscopic techniues based on high-resolution mass spectrometry (HR-ESIMS) and 1D/2D nuclear magnetic resonance (NMR). All isolated compounds were tested for their potential antimicrobial activity against four different bacterial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa), in addition to a fungal strain (Candida tropicalis), The results were expressed as the diameter of the clear zone (in millimetres) around each well. Compounds 1 and 3 (isochlorogenic acid A-3′-O-β-glucopyranoside and isochlorogenic acid C-3′-O-β-glucopyranoside, respectively) displayed remarkable antifungal effect and potent antibacterial activities against B. subtilis and S. aureus, respectively. 3α,4α-10β-trihydroxy-8α-acetyloxyguaian-12,6α-olide (6) and angelicoidenol 2-O-β-d-glucopyranoside (9) emerged as interesting dual antibacterial (selective on P. aeruginosa)/antifungal agents.
Ghanwa Riaz, Amna Shoaib, Sidra Javed, Shagufta Perveen, Waseem Ahmed, Mohamed A. El-Sheikh, and Prashant Kaushik
Elsevier BV
Eman Fikry, Raha Orfali, Samar S. Elbaramawi, Shagufta Perveen, Azza M. El-Shafae, Maher M. El-Domiaty, and Nora Tawfeek
MDPI AG
Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and more effective alternatives, such as natural plant products. Essential oils (EOs) are one such alternative, offering a wide range of bioactivities, including antibacterial, antiviral, antioxidant, and anticancer properties. Accordingly, the objective of the present study was to investigate the chemical composition, as well as the antioxidant and anticancer properties of the leaf essential oil of Chamaecyparis lawsoniana (CLLEO) belonging to the Cupressaceae family. Totally, 59 constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. cis-Abienol, trans-ferruginol, α-cadinol, δ-muurolene and α-pinene were the major components. The in vitro cytotoxicity study against human breast (MCF-7), colon (HCT-116), lung (A-549), hepatocellular (HepG-2) carcinoma cells using MTT assay indicated a promising cytotoxic activity against all the tested cancer cells, particularly HepG-2, with significant selectivity indices. CLLEO exhibited weak antioxidant activity according to the DPPH, ABTS and FRAP assays. In silico docking of these constituents against the epidermal growth factor receptor (EGFR), the myeloid cell leukemia-1 (Mcl-1) and caspase-8 using Molecular Operating Environment (MOE) software demonstrated good binding affinities of the components with the active site of these targets. These findings suggested using CLLEO, or its individual components, as a potentially viable therapeutic option for managing cancerous conditions.
Saifullah Bullo, Rifat Jawaria, Iqra Faiz, Iqra Shafiq, Muhammad Khalid, Muhammad Adnan Asghar, Rabia Baby, Raha Orfali, and Shagufta Perveen
American Chemical Society (ACS)
Currently, we reported the synthesis of six novel salicylaldehyde-based thiosemicarbazones (BHCT1–HBCT6) via condensation of salicylaldehyde with respective thiosemicarbazide. Through various spectroscopic methods, UV–visible and NMR, the chemical structures of BHCT1–HBCT6 compounds were determined. Along with synthesis, a computational study was also performed at the M06/6-31G(d,p) functional. Various analyses such as natural bond orbital (NBO) analysis, natural population analysis, frontier molecular orbital (FMO) analysis, and molecular electrostatic potential surfaces were carried out to understand the nonlinear optical (NLO) characteristics of the synthesized compounds. Additionally, a comparative study was carried out between DFT and experimental results (UV–vis study), and a good agreement was observed in the results. The energy gap calculated through FMOs was found to be in decreasing order as 4.505 (FHCT2) > 4.499 (HBCT6) > 4.497 (BHCT1) = 4.497(HMCT5) > 4.386 (CHCT3) > 4.241(AHCT4) in eV. The global reactivity parameters (GRPs) were attained through EHOMO and ELUMO, which described the stability and hardness of novel compounds. The NBO approach confirmed the charge delocalization and stability of the molecules. Among all the investigated compounds, a larger value (557.085 a.u.) of first hyperpolarizability (βtot) was possessed by CHCT3. The NLO response (βtot) of BHCT1–HBCT6 was found to be 9.145, 9.33, 13.33, 5.43, 5.68, and 10.13 a.u. times larger than that of the standard para-nitroaniline molecule. These findings ascertained the potential of entitled ligands as best NLO materials for a variety of applications in modern technology.