MUC1/CA15-3 identifies a clear cell renal carcinoma characterized by Sunitinib response with a specific metabolic signature Giuseppe Lucarelli, Francesco Lasorsa, Martina Milella, Antonio d’Amati, Giuseppe Ingravallo, Antonio Di Bari, Savio Domenico Pandolfo, Roberto Tamma, Michela De Giorgis, Domenico Ribatti, Annalisa Schirinzi, Francesca di Serio, Alessandro Caniglia, Francesco Alfredo Zito, Emanuele Naglieri, Michele Battaglia, Pasquale Ditonno, Monica Rutigliano Clinical and Experimental Medicine, 2026 Clear cell renal carcinoma (ccRCC) is a prevalent kidney cancer with limited effective biomarkers for prognosis and treatment guidance. Despite advancements, a significant portion of ccRCC cases progress to advanced stages, necessitating novel diagnostic tools. Here, we investigated the expression of MUC1 and its soluble form, CA15-3, in ccRCC, evaluating their potential as biomarkers for angiogenesis and response to sunitinib therapy. Molecular analyses showed that MUC1 expression was associated with angiogenesis, epithelial–mesenchymal transition, hypoxia/metabolism regulation, and complement system activation. In particular MUC1 overexpression correlated with increased microvascular density in vitro and in vivo models. Elevated CA15-3 levels were associated with tumor burden and predict clinical response to sunitinib in metastatic ccRCC. Metabolomic analysis showed that sunitinib-responding tumors were characterized by specific metabolic changes involving glucose and lipid metabolism, in association with impaired oxidative phosphorylation. MUC1 expressing ccRCC is a high angiogenic tumor that presents characteristics of increased aggressiveness, and a specific metabolic profile. Serum CA15-3 is a marker of poor survival and predicts response of sunitinib in patients with metastatic disease.
Aortic Growth After Isolated Aortic Valve Repair in Patients With Aortic Regurgitation Without Primary Aneurysm Indication Francesco Zito, Johanna J M Takkenberg, Giovanni Melina, Emmanuel Lansac, Laurent de Kerchove, Jan Vojáček, Igor Rudez, Peter Verbrugghe, Vincent Chauvette, Claudia Romagnoni, Marek Jasinski, Adrián Kolesár, Raffaele Scaffa, Jolanda Kluin, Kevin M Veen, Bardia Arabkhani, , Jaroslav Hlubocky, Bardia Arabkhani, Maciej Matuszewski, Alejandro Crespo de Hubsch, Pallav Shah, Gianclaudio Mecozzi, Olivier Bouchot, Takeshi Miyairi, Aayush Poddar, Carlotta Brega, Vladislav Aminov, Chaud German, Eric Bergoend, Evaldas Girdauskas, Ignacio Bibiloni, Carlos Porras European Journal of Cardio Thoracic Surgery, 2026
LncRNA expression in prostate cancer: From in silico analysis to urinary EV-based liquid biopsy for prognosis Simona De Summa, Letizia Porcelli, Giuseppe De Palma, Antonio Palazzo, Giuseppina Matera, Alessandro Caniglia, Roberta Di Fonte, Rosella Fasano, Francesco A. Zito, Stefania Tommasi, Simona Serratì, Amalia Azzariti Non Coding RNA Research, 2026 Prostate cancer (PCa) is the most frequently diagnosed malignancy among men and remains a leading cause of cancer-related death. Despite the widespread use of diagnostic tools such as PSA testing and Gleason scoring, these methods often fall short in accurately predicting tumor progression, highlighting the urgent need for reliable, non-invasive biomarkers to improve prognosis. In this study, we explored the potential of long non-coding RNAs (lncRNAs) as novel prognostic biomarkers for PCa. An initial in silico analysis of public datasets identified six lncRNAs differentially expressed in PCa tissues compared to healthy and adjacent non-tumor tissues. Of these, three were validated in a cohort of prostate cancer and benign prostatic hyperplasia (BPH) tissue samples. Notably, four lncRNAs showed expression levels that increased with higher Gleason scores, suggesting their potential as indicators of disease progression rather than early detection. To evaluate their applicability in a non-invasive setting, we assessed the expression of these lncRNAs in urinary extracellular vesicles (EVs). The results evidenced that Lnc-OPRK1-3 and lnc-SRSF6-1 are candidates as non-invasive prognostic biomarkers since they were detectable in urine and exhibited expression levels that correlate with advancing disease, specifically in the transition from Gleason score 3 + 3 to 4 + 3 and above. Their detection through urine-based liquid biopsy could support more precise patient stratification and suggest clinical decision-making. Further investigation is needed to validate their functional relevance and clinical utility.
Biological and prognostic relevance of A-to-I RNA editing across consensus molecular subtypes of colon cancer Dario Monaco, Debora Traversa, Eliseo Mattioli, Francesco Alfredo Zito, Grazia Cristiani, Francesca Buono, Sabina Delcuratolo, Tiziana Guarino, Rosamaria Pinto, Antonia Lasorella, Rosanna Lacalamita, Ernesto Picardi, Stefania Tommasi, Simona De Summa Scientific Reports, 2026 Colorectal cancer (CRC) is the second most frequently diagnosed cancer worldwide and represents a major challenge for public health. Despite advances in molecular profiling, important gaps remain in our understanding of tumorigenesis and the regulatory mechanisms underlying CRC progression. The most widely adopted classification system is the Consensus Molecular Subtypes (CMS), which stratifies CRC into four biologically distinct subtypes. We investigated the role of A-to-I RNA editing across CMS subtypes in a cohort of 100 CRC patients at various disease stages. Bulk RNA-seq data were analyzed using REDItools to detect editing events, focusing on both recoding sites and edits within repetitive elements, such as Alu sequences. Furthermore, expression levels of the ADAR enzyme family were assessed, and deconvolution analyses were performed on single-cell RNA-seq data from an independent cohort of stage II CRC patients to characterize editing activity within the tumor microenvironment (TME). Competitive endogenous RNA (ceRNA) networks, specific to each CMS subtype, were constructed based on editing events in repetitive elements. A multivariate Cox proportional hazards model was applied to evaluate associations with overall survival (OS). We observed statistically significant differences in ADARB1 expression across CMS subtypes. Single-cell RNA-seq data revealed subtype-specific distribution patterns of ADAR enzymes within the TME. Analysis of editing events showed subtype-specific signatures in both known cancer-related genes (e.g., COPA, CADPS, IGFBP7) and novel candidates (ZNF552, RALGPS1). Editing in repetitive elements informed the construction of distinct ceRNA networks for each CMS subtype, suggesting different post-transcriptional regulatory mechanisms. Survival analysis identified three variables significantly associated with OS, independent of CMS classification and clinical stage: ADARB1 expression, and editing events in NOP14-AS1 (chr4:2960236; p = 0.036; HR = 0.0069), previously linked to 5-FU sensitivity, and ST7-AS2 (chr4:117120557). This study underscores the biological relevance of RNA editing in CRC, highlighting its impact on chemoresistance, the tumor microenvironment, and subtype-specific gene regulation. Our findings suggest that RNA editing represents a critical post-transcriptional regulatory layer in CRC and holds potential as a biomarker and therapeutic target.
Innovation in aortic surgery requires more than AI: a perspective on data governance and shared decision-making Adine R. de Keijzer, Reda Rhellab, Francesco Zito, Jolanda Kluin, Giovanni Melina, Johanna J. M. Takkenberg, Kevin M. Veen Vessel Plus, 2026 Clinical decision-making for individuals undergoing valvular and aortic surgery remains challenging, particularly in young patients facing lifelong risk and repeated interventions. As predictive technologies such as artificial intelligence and advanced statistical modelling evolve, the surgical community must ensure that key foundational elements, namely data governance, including data standardization, and regulation, are firmly in place. Without high-quality, standardized, and ethically governed data, predictive models risk offering misleading guidance rather than meaningful personalization. While data governance ensures the scientific robustness of predictive technologies, shared decision-making (SDM) ensures these innovations remain closely aligned with the lived experiences, values, and preferences of individual patients. This perspective emphasizes that advancing decision-making in aortic surgery requires deliberate investment in building data ecosystems and fostering SDM communication practices, ensuring that innovation is both scientifically sound and truly responsive to patient needs.
Predicting modelling for early diagnosis in early myeloproliferative neoplasms, not otherwise specified. Evidence from a machine learning study Anna Scattone, Andrea Lupo, Concetta Saponaro, Margherita Sonnessa, Paolo Ditonno, Samantha Bove, Annarita Fanizzi, Giuseppe Accogli, Rossana Daprile, Francesco Alfredo Zito, Maria Colomba Comes, Raffaella Massafra Frontiers in Oncology, 2026 Background Recently, artificial intelligence (AI) has become a valuable tool for diagnosing and predicting outcomes in blood disorders. Whole Slide Imaging (WSI) of bone marrow biopsies (BMBs) offers detailed, high-resolution views of cells and tissues; its adoption may improve the resources dedicated to the interpretation of BMB with suspected early myeloproliferative neoplasms (MPNs). Methods We collected a retrospective dataset of H&E-stained BMBs from 88 patients diagnosed with MPN, divided into three groups: 19 with prefibrotic primary myelofibrosis (pre-PMF), 30 with polycythemia vera (PV), and 39 with essential thrombocythemia (ET). Using AI, we framed this as a three-class classification problem. For each whole slide image, we automatically calculated cellularity and cell density. We extracted morphological features related to megakaryocytes—area, perimeter, and circularity—and summarized them for each patient using statistics like mean, standard deviation, skewness, kurtosis, and entropy. This resulted in 17 features combining nuclear morphology, cell density statistics, and cellularity. After selecting significant features with the Kruskal-Wallis test, we trained a Support Vector Machine (SVM) classifier with 5-fold cross-validation to predict MPN subtypes. We then tested the model on 13 patients with a diagnosis of MPN not otherwise specified (MPN-NOS) to assess its capacity to correctly characterize the diagnosis. Results The model achieved a mean multiclass Area Under the Curve (AUC) of 0.70 ± 0.01 and an accuracy of 0.60 ± 0.03. When tested on the 13 patients with MPN not otherwise specified (MPN-NOS), the model agreed with pathologists’ biopsy classifications 61.5% of the time and with clinical follow-up evaluations 50% of the time. Conclusions This study represents a first step toward the development of automated tools to support MPN diagnosis, providing potential assistance to haematologists and pathologists in the clinical management of patients.
Enhancing early prediction of pathological complete response in breast cancer using attention-based convolutional neural networks in digital pathology Maria Colomba Comes, Andrea Lupo, Arianna Bozzi, Annarita Fanizzi, Angelo Cirillo, Giorgio De Nunzio, Maria Irene Pastena, Alessandro Rizzo, Deniz Can Guven, Elsa Vitale, Francesco Alfredo Zito, Samantha Bove, Raffaella Massafra Digital Health, 2026 Objective To develop an attention-based convolutional neural network (CNN) pipeline for early prediction of pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) in breast cancer, improving feature selection and interpretability in whole slide image (WSI) analysis. Methods A retrospective analysis was conducted on 384,076 tiles extracted from 122 Hematoxylin and Eosin-stained WSIs, divided among an investigational cohort (IC, 82 patients enrolled at IRCCS Istituto Tumori “Giovanni Paolo II”), a validation cohort (VC, 20 patients, same Institution), and an external validation cohort (EVC, 20 patients belonging the Yale trastuzumab response cohort public dataset). WSIs were first annotated and then automatically segmented into tiles. Noninformative regions were filtered using Mini-Batch C-Fuzzy K-Means. Remaining tiles were analyzed using a CNN with a Convolutional Block Attention Module, prioritizing both histological features and tiles critical for predicting pCR. Results The model achieved robust performance: 81.4% AUC, 81.3% accuracy, 80.0% specificity, and 83.3% sensitivity in IC; 80.9% AUC, 80.0% accuracy, 85.78% specificity, and 66.7% sensitivity in VC; and 76.2% AUC, 70.0% accuracy, 71.4% specificity, and 66.7% sensitivity in EVC. The EVC, consisting of WSIs at 20× magnification compared to the 40× magnification of IC and VC, demonstrated the model's robustness to varying resolutions. Conclusion This is an innovative pipeline that not only improves prediction but also enhances the clinical utility of digital pathology.
Multidisciplinary discussion on phenotypical characterization of metastatic breast cancer and patient pathway optimization from an expert panel across South Italian regions Raffaele Ardito, Grazia Arpino, Roberta Caputo, Mariangela Ciccarese, Sabino De Placido, Francesco Giotta, Rosamaria Iommelli, Mihaela Nica, Michele Orditura, Daniela Russo, Luigia Stefania Stucci, Francesco Alfredo Zito Scientific Reports, 2025 Breast cancer (BC) might change its receptor status during malignant progression, resulting in challenging clinical care. The project, developed during two residential meetings by expert Italian oncologists and pathologists, was aimed at optimizing metastatic BC management. A 17-point survey was administered to healthcare workers in centers of three regions, essentially to assess the perception of metastasis biopsy on treatment choice in different BC molecular subtypes, the appropriate timing for performing tissue and liquid biopsy in metastatic BC, the selection of target genes and the use of multidisciplinary approaches in tumour management. Nineteen centers participated in the survey. The first important finding was that most centers manage more than 150 patients yearly, and 95% have a pathology department. Some questions received a substantial agreement: tissue biopsy of metastatic BC was largely considered as mandatory, generally performed at onset of metastatic disease. Liquid biopsy is not used by all centers, confirming its strict dependency on the available technologies. The answers on the therapeutic line change in case of HER2 + disease becoming triple negative confirmed an attitude consistent with clinical practice. The survey also revealed that half of the interviewed oncologists discuss with multidisciplinary team only for selected cases. The survey revealed that while a shared consensus exists on the importance of metastasis biopsy, some areas still deserve attention to achieve standardized approaches: interdisciplinary collaboration and more effective communication between specialists and patients are necessary to optimize the diagnostic and therapeutic journey of women with metastatic BC.
miR-451 Is a Driver of Lipotoxic Injury in Patients with Diabetic Cardiomyopathy Sarah Costantino, Shafeeq A. Mohammed, Federico Ranocchi, Francesco Zito, Valentina Delfine, Nazha Hamdani, Maria Cristina Vinci, Giovanni Melina, Francesco Paneni Cells, 2025 MicroRNA 451 (miR-451) is emerging as a pivotal mediator of cardiac damage in experimental models of diabetic cardiomyopathy. Whether miR-451 plays a detrimental role in the human diabetic myocardium is unknown. The present study investigates miR-451’s role in patients with type 2 diabetes (T2D). We show that miR-451 is upregulated in myocardial specimens from T2D patients compared to controls without diabetes and correlates with cardiometabolic parameters, the myocardial triglyceride content and cardiac expression of lipotoxic genes as well as echocardiographic indices of left ventricular dysfunction. Calcium-binding protein 39 (Cab39)—a known target of miR-451 in mouse hearts—was downregulated in T2D patients vs. controls, and its expression negatively correlated with that of miR-451. In cultured human cardiomyocytes (CMs), Ago2 immunoprecipitation confirmed Cab39 to be a direct target of miR-451. Treatment with a high amount of glucose (25mM) and palmitic acid (PA) mimicked miR-451 upregulation and Cab39 downregulation in human CMs. These changes were associated with increased TGs and markers of lipotoxic injury, such as elevated oxidative stress levels, mitochondrial dysfunction and apoptosis. Targeting miR-451 led to restoration of Cab39 levels while rescuing diabetes-induced lipotoxic injury and metabolic dysfunction. By contrast, miR-451 overexpression recapitulated features of lipotoxic damage. Our findings indicate miR-451 to be a potential target for the prevention of myocardial lipotoxic injury in diabetes.
L1CAM Reliably Distinguishes Low-Grade Oncocytic Tumor from Other Eosinophilic Renal Neoplasms: A Multicenter Immunohistochemical Study with Diagnostic Implications Luciana Scuccimarri, Antonio d’Amati, Francesco Pierconti, Angela Santoro, Luigia Ciampi, Tiziana Montrone, Francesco Alfredo Zito, Giuseppe Lucarelli, Guido Rindi, Gian Franco Zannoni, Mauro Giuseppe Mastropasqua Cancers, 2025 Introduction: Low-grade oncocytic tumor (LOT) is a recently described renal neoplasm characterized by indolent clinical behavior, a small nested architecture, and distinctive immunophenotypic features. Its distinction from other eosinophilic renal tumors, such as oncocytoma, eosinophilic chromophobe renal cell carcinoma (E-chRCC), and eosinophilic vacuolated tumor (EVT), can be challenging due to overlapping features. The L1 cell adhesion molecule (L1CAM) is being increasingly recognized as a potential diagnostic marker for LOT. Aims: To evaluate the diagnostic performance of L1CAM in distinguishing LOT from morphologically and immunophenotypically similar eosinophilic renal neoplasms. Methods: A total of 54 eosinophilic renal tumors (10 LOTs, 22 oncocytomas, 18 E-chRCCs, and 4 EVTs) were retrospectively collected from five academic institutions and reclassified according to the 2022 WHO criteria. All cases underwent histopathologic review and immunohistochemical analysis for CK7, CD117, GATA3, cathepsin K, and L1CAM. Results: L1CAM showed strong membranous expression in all LOTs (100%) and was negative in oncocytoma, E-chRCC, and EVT, yielding 100% sensitivity and specificity. Traditional markers exhibited overlapping patterns among tumor types. Conclusions: Our findings confirm L1CAM as a highly sensitive and specific marker for LOT, effectively distinguishing it from other eosinophilic renal neoplasms. Incorporating L1CAM into diagnostic panels may enhance accuracy, particularly in challenging cases.
Insight into the Regulation of NDRG1 Expression Concetta Saponaro, Nicola Gammaldi, Viviana Cavallo, Maria Antonieta Ramírez-Morales, Francesco Alfredo Zito, Margherita Sonnessa, Francesco Vari, Ilaria Serra, Simona De Summa, Anna Maria Giudetti, Marco Trerotola, Daniele Vergara International Journal of Molecular Sciences, 2025
Trends in Thyroid Fine-Needle Aspiration Cytology - Results from the Italian Cytopathology Committee National Practice Survey Gennaro Acanfora, , Mariantonia Nacchio, Carla Baronchelli, Amedeo Boscaino, Carolina Buriani, Elisabetta Carico, Andrea Cavazzana, Anna Maria Cesinaro, Doglioni Claudio, Immacolata Cozzolino, Anna Crescenzi, Stefania Damiani, Giovanni De Chiara, Gisele de Rezende, Ludovica de Vincentiis, Guido Fadda, Gerardo Ferrara, Pironi Flavio, Giacomo Gazzano, Annarita Gencarelli, Francesca Grillo, Leo Guidobaldi, Maria Letizia Lai, Gaetano Magro, Grazia Marangi, Fabio Pagni, Gianmaria Pennelli, Simonetta Piana, Michele Pirrelli, Stefano Pizzolitto, Giuseppina Renzulli, Esther Diana Rossi, Silvia Taccogna, Silvia Uccella, Clara Ugolini, Andrea Vecchione, Elena Vigliar, Marco Volante, Roberta Zappacosta, Francesco Alfredo Zito, Giovanni Tallini, Giancarlo Troncone, Claudio Bellevicine Pathologica, 2025
Unraveling the protein kinase C/NDRG1 signaling network in breast cancer C. Saponaro, M. Damato, E. Stanca, S. Aboulouard, F. A. Zito, S. De Summa, D. Traversa, L. Schirosi, S. Bravaccini, F. Pirini, E. Fonzi, M. Tebaldi, M. Puccetti, A. Gaballo, L. Pantalone, M. Ronci, L. Magnani, D. Sergi, A. Tinelli, S. Tacconi, L. Siculella, A. M. Giudetti, I. Fournier, M. Salzet, M. Trerotola, D. Vergara Cell and Bioscience, 2024
A targeted gene signature stratifying mediastinal gray zone lymphoma into classical Hodgkin lymphoma-like or primary mediastinal B-cell lymphoma-like subtypes Grazia Gargano, Maria Carmela Vegliante, Flavia Esposito, Susanna A. Pappagallo, Elena Sabattini, Claudio Agostinelli, Stefano A. Pileri, Valentina Tabanelli, Maurilio Ponzoni, Luisa Lorenzi, Fabio Facchetti, Arianna Di Napoli, Marco Lucioni, Marco Paulli, Lorenzo Leoncini, Stefano Lazzi, Stefano Ascani, Giuseppina Opinto, Gian Maria Zaccaria, Giacomo Volpe, Paolo Mondelli, Antonella Bucci, Laura Selicato, Antonio Negri, Giacomo Loseto, Felice Clemente, Anna Scattone, Alfredo F. Zito, Luca Nassi, Nicoletta Del Buono, Attilio Guarini, Sabino Ciavarella Haematologica, 2024
BRCA testing in metastatic castration-resistant prostate cancer: successes and troubles in a real world setting. An Italian Multicentric study Stefania Tommasi, Claudio Antonio Coppola, Alessandro Caniglia, Brunella Pilato, Francesco Alfredo Zito, Mariantonia Carosi, Elisa Melucci, Beatrice Casini, Andrea Russo, Viviana Gismondi, Gabriella Cirmena, Michele Paudice, Umberto Malapelle, Francesco Pepe, Giancarlo Troncone, Gabriella Fontanini, Rossella Bruno, Pinuccia Faviana, Davide Vacirca, Sergio Vincenzo Taormina, Simona Francesconi, Cecilia Caprera, Matteo Corsi, Sergio Bracarda, Massimo Barberis Pathologica, 2024
Native Aortic Valve Resection Using a Novel Blade-Based Device Emiliano Navarra, Xavier Bollen, Francesco Zito, Laurent de Kerchove, Gebrine El Khoury, Astarci Parla Innovations Technology and Techniques in Cardiothoracic and Vascular Surgery, 2024
The molecular tumor board as a step in cancer patient management: a southern Italian experience Stefania Tommasi, Leonarda Maurmo, Alessandro Rizzo, Claudia Carella, Girolamo Ranieri, Simona De Summa, Francesco Mannavola, Vincenzo Emanuele Chiurì, Michele Guida, Claudia Nisi, Michele Montrone, Francesco Giotta, Margherita Patruno, Rosanna Lacalamita, Brunella Pilato, Francesco Alfredo Zito, Livia Fucci, Claudio Antonio Coppola, Paolo Ditonno, Patrizia Nardulli, Davide Quaresmini, Sabino Strippoli Frontiers in Medicine, 2024
Evaluation of human papillomavirus DNA in colorectal cancer and adjacent mucosal tissue samples Luisa Galati, Purnima Gupta, Antonio Tufaro, Mariarosaria Marinaro, Concetta Saponaro, David Israel Escobar Marcillo, Donato Loisi, Rajdip Sen, Alexis Robitaille, Rosario N. Brancaccio, Cyrille Cuenin, Sandrine McKay-Chopin, Angelo Virgilio Paradiso, Václav Liška, Pavel Souček, Francesco Alfredo Zito, David J. Hughes, Massimo Tommasino, Tarik Gheit Infectious Agents and Cancer, 2023
VEGFA Status as a Predictive Marker of Therapy Outcome in Metastatic Gastric Cancer Patients Following Ramucirumab-Based Treatment Annalisa Schirizzi, Aram Arshadi, Doron Tolomeo, Laura Schirosi, Anna Maria Valentini, Giampiero De Leonardis, Maria Grazia Refolo, Rossella Donghia, Clelia Tiziana Storlazzi, Alfredo Zito, Angela Dalia Ricci, Simona Vallarelli, Carmela Ostuni, Maria Bencivenga, Giovanni De Manzoni, Caterina Messa, Raffaele Armentano, Gianluigi Giannelli, Claudio Lotesoriere, Rosalba D’Alessandro Biomedicines, 2023
circPVT1 and PVT1/AKT3 show a role in cell proliferation, apoptosis, and tumor subtype-definition in small cell lung cancer Doron Tolomeo, Debora Traversa, Santina Venuto, Karoline K. Ebbesen, Juan L. García Rodríguez, Grazia Tamma, Marianna Ranieri, Giorgia Simonetti, Martina Ghetti, Matteo Paganelli, Grazia Visci, Arcangelo Liso, Klaas Kok, Lucia Anna Muscarella, Federico Pio Fabrizio, Maria Antonia Frassanito, Aurelia Lamanuzzi, Ilaria Saltarella, Antonio Giovanni Solimando, Alessandro Fatica, Zaira Ianniello, René Massimiliano Marsano, Antonio Palazzo, Amalia Azzariti, Vito Longo, Stefania Tommasi, Domenico Galetta, Annamaria Catino, Alfredo Zito, Tommaso Mazza, Alessandro Napoli, Giovanni Martinelli, Jørgen Kjems, Lasse Sommer Kristensen, Angelo Vacca, Clelia Tiziana Storlazzi Genes Chromosomes and Cancer, 2023
Impact of the systematic introduction of tomosynthesis on breast biopsies: 10 years of results Daniele La Forgia, Rahel Signorile, Samantha Bove, Francesca Arezzo, Gennaro Cormio, Antonella Daniele, Miriam Dellino, Annarita Fanizzi, Gianluca Gatta, Miria Lafranceschina, Raffaella Massafra, Alessandro Rizzo, Francesco Alfredo Zito, Emanuele Neri, Lorenzo Faggioni Radiologia Medica, 2023
NR1H3 (LXRα) is associated with pro-inflammatory macrophages, predicts survival and suggests potential therapeutic rationales in diffuse large b-cell lymphoma Maria Carmela Vegliante, Saveria Mazzara, Gian Maria Zaccaria, Simona De Summa, Flavia Esposito, Federica Melle, Giovanna Motta, Maria Rosaria Sapienza, Giuseppina Opinto, Giacomo Volpe, Antonella Bucci, Grazia Gargano, Anna Enjuanes, Valentina Tabanelli, Stefano Fiori, Carla Minoia, Felice Clemente, Antonio Negri, Alessandro Gulino, Gaia Morello, Anna Scattone, Alfredo F. Zito, Stefania Tommasi, Claudio Agostinelli, Umberto Vitolo, Annalisa Chiappella, Anna Maria Barbui, Enrico Derenzini, Pier Luigi Zinzani, Beatrice Casadei, Alfredo Rivas‐Delgado, Armando López‐Guillermo, Elias Campo, Antonio Moschetta, Attilio Guarini, Stefano A. Pileri, Sabino Ciavarella Hematological Oncology, 2022
Accuracy of high-risk HPV DNA PCR, p16(INK4a) immunohistochemistry or the combination of both to diagnose HPV-driven oropharyngeal cancer Cindy Simoens, Tarik Gheit, Ruediger Ridder, Ivana Gorbaslieva, Dana Holzinger, Eric Lucas, Susanne Rehm, Peter Vermeulen, Martin Lammens, Olivier M. Vanderveken, Rekha Vijay Kumar, Nitin Gangane, Alessandro Caniglia, Fausto Maffini, Maria Belén Lloveras Rubio, Devasena Anantharaman, Susanna Chiocca, Paul Brennan, Madhavan Radhakrishna Pillai, Rengaswamy Sankaranarayanan, Johannes Bogers, Michael Pawlita, Massimo Tommasino, Marc Arbyn, the HPV-AHEAD study group, Christine Carreira, Sandrine McKay-Chopin, Rudrapatna S. Jayshree, Kortikere S. Sabitha, Ashok M. Shenoy, Alfredo Zito, Fausto Chiesa, Marta Tagliabue, Mohssen Ansarin, Subha Sankaran, Christel Herold-Mende, Gerhard Dyckhoff, George Mosialos, Heiner Boeing, Xavier Castellsagué, Silvia de Sanjosé, Marisa Mena, Francesc Xavier Bosch, Laia Alemany, Pulikottil Okkuru Esmy, Manavalan Vijayakumar, Aruna S. Chiwate, Ranjit V. Thorat, Girish G. Hublikar, Shashikant S. Lakshetti, Bhagwan M. Nene, Amal Ch. Kataki, Ashok Kumar Das, Kunnambath Ramadas, Thara Somanathan BMC Infectious Diseases, 2022
A roadmap towards breast cancer therapies supported by explainable artificial intelligence Nicola Amoroso, Domenico Pomarico, Annarita Fanizzi, Vittorio Didonna, Francesco Giotta, Daniele La Forgia, Agnese Latorre, Alfonso Monaco, Ester Pantaleo, Nicole Petruzzellis, Pasquale Tamborra, Alfredo Zito, Vito Lorusso, Roberto Bellotti, Raffaella Massafra Applied Sciences Switzerland, 2021
HPV DNA genotyping, HPV E6*I mRNA detection, and p16INK4a/Ki-67 staining in Belgian head and neck cancer patient specimens, collected within the HPV-AHEAD study Cindy Simoens, Ivana Gorbaslieva, Tarik Gheit, Dana Holzinger, Eric Lucas, Ruediger Ridder, Susanne Rehm, Peter Vermeulen, Martin Lammens, Olivier M. Vanderveken, Rekha Vijay Kumar, Nitin Gangane, Alessandro Caniglia, Fausto Maffini, Maria Belén Lloveras Rubio, Devasena Anantharaman, Susanna Chiocca, Paul Brennan, Madhavan Radhakrishna Pillai, Rengaswamy Sankaranarayanan, Johannes Bogers, Michael Pawlita, Massimo Tommasino, Marc Arbyn, Christel Herold-Mende, Gerhard Dyckhoff, George Mosialos, Fausto Chiesa, Marta Tagliabue, Mohssen Ansarin, Heiner Boeing, Xavier Castellsagué, Silvia de Sanjosé, Marisa Mena, Francesc Xavier Bosch, Laia Alemany, Pulikottil Okkuru Esmy, Rudrapatna S. Jayshree, Kortikere S. Sabitha, Ashok M. Shenoy, Manavalan Vijayakumar, Aruna S. Chiwate, Ranjit V. Thorat, Girish G. Hublikar, Shashikant S. Lakshetti, Bhagwan M. Nene, Amal Ch Kataki, Ashok Kumar Das, Subha Sankaran, Kunnambath Ramadas, Christine Carreira, Sandrine McKay-Chopin, Thara Somanathan, Alfredo Zito Cancer Epidemiology, 2021
A Clinical Decision Support System for Predicting Invasive Breast Cancer Recurrence: Preliminary Results Raffaella Massafra, Agnese Latorre, Annarita Fanizzi, Roberto Bellotti, Vittorio Didonna, Francesco Giotta, Daniele La Forgia, Annalisa Nardone, Maria Pastena, Cosmo Maurizio Ressa, Lucia Rinaldi, Anna Orsola Maria Russo, Pasquale Tamborra, Sabina Tangaro, Alfredo Zito, Vito Lorusso Frontiers in Oncology, 2021
Dissection of DLBCL microenvironment provides a gene expression-based predictor of survival applicable to formalin-fixed paraffin-embedded tissue S. Ciavarella, M.C. Vegliante, M. Fabbri, S. De Summa, F. Melle, G. Motta, V. De Iuliis, G. Opinto, A. Enjuanes, S. Rega, A. Gulino, C. Agostinelli, A. Scattone, S. Tommasi, A. Mangia, F. Mele, G. Simone, A.F. Zito, G. Ingravallo, U. Vitolo, A. Chiappella, C. Tarella, A.M. Gianni, A. Rambaldi, P.L. Zinzani, B. Casadei, E. Derenzini, G. Loseto, A. Pileri, V. Tabanelli, S. Fiori, A. Rivas-Delgado, A. López-Guillermo, T. Venesio, A. Sapino, E. Campo, C. Tripodo, A. Guarini, S.A. Pileri Annals of Oncology, 2018
A supervised CAD to support telemedicine in hematology Vitoantonio Bevilacqua, Domenico Buongiorno, Pierluigi Carlucci, Ferdinando Giglio, Giacomo Tattoli, Attilio Guarini, Nicola Sgherza, Giacoma De Tullio, Carla Minoia, Anna Scattone, Giovanni Simone, Francesco Girardi, Alfredo Zito, Loreto Gesualdo Proceedings of the International Joint Conference on Neural Networks, 2015
Duplication cyst of the stomach with respiratory epithelium in adult: An uncommon finding. Report of case and review of literature Annali Italiani Di Chirurgia, 2011
Expression of metalloproteinases MMP-2 and MMP-9 in sentinel lymph node and serum of patients with metastatic and non-metastatic breast cancer Anticancer Research, 2010
Biological role of NHERF1 protein expression in breast cancer Anita Mangia, Annalisa Chiriatti, Antonia Bellizzi, Andrea Malfettone, Baldassarre Stea, Francesso Alfredo Zito, Stephen Joel Reshkin, Giovanni Simone, Angelo Paradiso Histopathology, 2009
Changes in CpG islands promoter methylation patterns during ductal breast carcinoma progression Mohammad Obaidul Hoque, Maria Prencipe, Maria Luana Poeta, Raffaela Barbano, Vanna Maria Valori, Massimiliano Copetti, Antonietta Pia Gallo, Mariana Brait, Evaristo Maiello, Adolfo Apicella, Raffaele Rossiello, Francesco Zito, Tommasi Stefania, Angelo Paradiso, Massimo Carella, Bruno Dallapiccola, Roberto Murgo, Illuminato Carosi, Michele Bisceglia, Vito Michele Fazio, David Sidransky, Paola Parrella Cancer Epidemiology Biomarkers and Prevention, 2009
Biology, prognosis and response to therapy of breast carcinomas according to HER2 score S. Ménard, A. Balsari, E. Tagliabue, T. Camerini, P. Casalini, R. Bufalino, F. Castiglioni, M.L. Carcangiu, A. Gloghini, S. Scalone, P. Querzoli, M. Lunardi, A. Molino, M. Mandarà, M. Mottolese, F. Marandino, M. Venturini, C. Bighin, G. Cancello, E. Montagna, F. Perrone, A. De Matteis, A. Sapino, M. Donadio, N. Battelli, A. Santinelli, L. Pavesi, A. Lanza, F.A. Zito, A. Labriola, R.A. Aiello, M. Caruso, F. Zanconati, G. Mustacchi, M. Barbareschi, M. Frisinghelli, R. Russo, G. Carrillo Annals of Oncology, 2008
A rare case of extra-adrenal pheochromocytoma masquerading as an ovarian mass treated by laparoscopic surgery European Journal of Gynaecological Oncology, 2007
Gastrointestinal Stromal Tumours (GIST): Clinical features and anatomical pathology Giornale Italiano Di Endoscopia Digestiva, 2007
Cytoskeleton and paclitaxel sensitivity in breast cancer: The role of β-tubulins Stefania Tommasi, Anita Mangia, Rosanna Lacalamita, Antonia Bellizzi, Vita Fedele, Annalisa Chiriatti, Christopher Thomssen, Nancy Kendzierski, Agnese Latorre, Vito Lorusso, Francesco Schittulli, Francesco Zito, Maria Kavallaris, Angelo Paradiso International Journal of Cancer, 2007
Multicenter validation of a gene expression-based prognostic signature in lymph node-negative primary breast cancer John A. Foekens, David Atkins, Yi Zhang, Fred C.G.J. Sweep, Nadia Harbeck, Angelo Paradiso, Tanja Cufer, Anieta M. Sieuwerts, Dmitri Talantov, Paul N. Span, Vivianne C.G. Tjan-Heijnen, Alfredo F. Zito, Katja Specht, Heinz Hoefler, Rastko Golouh, Francesco Schittulli, Manfred Schmitt, Louk V.A.M. Beex, Jan G.M. Klijn, Yixin Wang Journal of Clinical Oncology, 2006
Primary tumours neoangiogenesis and p53 expression in oral carcinoma patients Journal of Experimental and Clinical Cancer Research, 2001
[Mandibular metastases from rectal adenocarcinoma: clinical case report and review of the literature]. Acta Otorhinolaryngologica Italica Organo Ufficiale Della Societa Italiana Di Otorinolaringologia E Chirurgia Cervico Facciale, 2001
Tamoxifen and female genital diseases. General considerations and clinical experience Giornale Italiano Di Ostetricia E Ginecologia, 1998
Infiltrating cribriform carcinoma of the breast. A clinico-pathologic and immunohistochemical study of 5 cases European Journal of Gynaecological Oncology, 1996
Ovarian fibroma - A report of three cases European Journal of Gynaecological Oncology, 1995
A modified cell block technique for fine needle aspiration cytology Acta Cytologica, 1995
Flow cytometry DNA content and morphobiological characteristics in chronic and neoplastic human liver disease Italian Journal of Gastroenterology, 1995
Consensus molecular subtyping in colon cancer: from the design of in-house assay to prognostic nomogram M Caputo, L Maurmo, D Traversa, TM Marvulli, E Mattioli, FA Zito, ... 2026
LncRNA expression in prostate cancer: From in silico analysis to urinary EV-based liquid biopsy for prognosis S De Summa, L Porcelli, G De Palma, A Palazzo, G Matera, A Caniglia, ... Non-coding RNA Research 16, 117-125 , 2026 2026
Predicting modelling for early diagnosis in early myeloproliferative neoplasms, not otherwise specified. Evidence from a machine learning study A Scattone, A Lupo, C Saponaro, M Sonnessa, P Ditonno, S Bove, ... Frontiers in Oncology 15, 1741610 , 2026 2026 Citations: 1
MUC1/CA15-3 identifies a clear cell renal carcinoma characterized by Sunitinib response with a specific metabolic signature G Lucarelli, F Lasorsa, M Milella, A d’Amati, G Ingravallo, A Di Bari, ... Clinical and Experimental Medicine , 2026 2026 Citations: 5
Biological and prognostic relevance of A-to-I RNA editing across consensus molecular subtypes of colon cancer D Monaco, D Traversa, E Mattioli, FA Zito, G Cristiani, F Buono, ... Scientific Reports , 2026 2026 Citations: 2
Anna Scattone1, Andrea Lupo2, Concetta Saponaro1, Margherita Sonnessa1, Paolo Ditonno3, Samantha Bove2, Annarita Fanizzi2, Giuseppe Accogli2, Rossana Daprile1, Francesco … A Scattone, A Lupo, C Saponaro, M Sonnessa, P Ditonno, S Bove, ... 2026
Enhancing early prediction of pathological complete response in breast cancer using attention-based convolutional neural networks in digital pathology MC Comes, A Lupo, A Bozzi, A Fanizzi, A Cirillo, GD Nunzio, MI Pastena, ... Digital Health 12, 20552076261419242 , 2026 2026 Citations: 1
Trends in Thyroid Fine-Needle Aspiration Cytology-Results from the Italian Cytopathology Committee National Practice Survey G Acanfora, M Nacchio, C Baronchelli, A Boscaino, C Buriani, E Carico, ... Pathologica 117 (4), 309 , 2025 2025 Citations: 1
A-to-I RNA editing across consensus molecular subtypes of colon cancer: biological insights and prognostic model D Monaco, D Traversa, E Mattioli, FA Zito, G Cristiani, F Buono, ... 2025
Trends in Thyroid Fine-Needle Aspiration Cytology-Results from the Italian Cytopathology Committee National Practice Survey: Trends in Thyroid FNAC–2022 Italian Survey G Acanfora, M Nacchio, C Baronchelli, A Boscaino, C Buriani, E Carico, ... Pathologica-Journal of the Italian Society of Anatomic Pathology and … , 2025 2025
L1CAM expression defines low-grade oncocytic tumours among eosinophilic renal neoplasms A d'Amati, L Scuccimarri, F Pierconti, A Santoro, L Ciampi, T Montrone, ... VIRCHOWS ARCHIV 487, S547-S548 , 2025 2025
Microclimatic Parameters, Soil Quality, and Crop Performance of Lettuce, Pepper, and Chili Pepper as Affected by Modified Growing Conditions in a Photovoltaic Plant: A Case … V Tucci, FF Montesano, GM Altieri, G Bari, E Tarasco, F Zito, S Strazzella, ... Agronomy 15 (9), 2035 , 2025 2025 Citations: 2
L1CAM Reliably Distinguishes Low-Grade Oncocytic Tumor from Other Eosinophilic Renal Neoplasms: A Multicenter Immunohistochemical Study with Diagnostic Implications L Scuccimarri, A d’Amati, F Pierconti, A Santoro, L Ciampi, T Montrone, ... Cancers 17 (15), 2440 , 2025 2025 Citations: 3
Multidisciplinary discussion on phenotypical characterization of metastatic breast cancer and patient pathway optimization from an expert panel across South Italian regions R Ardito, G Arpino, R Caputo, M Ciccarese, S De Placido, F Giotta, ... Scientific Reports 15 (1), 16029 , 2025 2025 Citations: 1
Insight into the Regulation of NDRG1 Expression C Saponaro, N Gammaldi, V Cavallo, MA Ramírez-Morales, FA Zito, ... International Journal of Molecular Sciences 26 (8), 3582 , 2025 2025 Citations: 8
SolarFertigation: A Unified Cloud Platform for Smart Fertigation and Agrivoltaic System Integration F Zito, NI Giannoccaro, S Strazzella, V Tucci BIO Web of Conferences 199, 01002 , 2025 2025 Citations: 1
The HER2 Regulatory Network: Elucidating Heterogeneity, Key Nodes and Interactions S De Summa, M Sonnessa, FA Zito, L Schirosi, C Saponaro Italian Journal of Anatomy and Embryology 129, 172-172 , 2025 2025
Unraveling the protein kinase C/NDRG1 signaling network in breast cancer C Saponaro, M Damato, E Stanca, S Aboulouard, FA Zito, S De Summa, ... Cell & Bioscience 14 (1), 156 , 2024 2024 Citations: 4
Monitoring over time of pathological complete response to neoadjuvant chemotherapy in breast cancer patients through an ensemble vision transformers‐based model MC Comes, A Fanizzi, S Bove, L Boldrini, A Latorre, DC Guven, ... Cancer medicine 13 (24), e70482 , 2024 2024 Citations: 7
HIV‐negative HHV8‐positive multicentric Castleman's disease coexistent with atypical Kaposi's sarcoma A Scattone, G Loseto, BG Mennuni, V Mastrandrea, R Buquicchio, C Foti, ... JEADV Clinical Practice 3 (5), 1606-1610 , 2024 2024
MOST CITED SCHOLAR PUBLICATIONS
Cytoskeleton and paclitaxel sensitivity in breast cancer: the role of β‐tubulins S Tommasi, A Mangia, R Lacalamita, A Bellizzi, V Fedele, A Chiriatti, ... International journal of cancer 120 (10), 2078-2085 , 2007 2007 Citations: 226
Tryptase-positive mast cells correlate with angiogenesis in early breast cancer patients G Ranieri, M Ammendola, R Patruno, G Celano, FA Zito, S Montemurro, ... International journal of oncology 35 (1), 115-120 , 2009 2009 Citations: 120
Positive predictive value for malignancy on surgical excision of breast lesions of uncertain malignant potential (B3) diagnosed by stereotactic vacuum-assisted needle core … S Bianchi, S Caini, G Renne, E Cassano, D Ambrogetti, MG Cattani, ... The Breast 20 (3), 264-270 , 2011 2011 Citations: 119
Changes in CpG islands promoter methylation patterns during ductal breast carcinoma progression MO Hoque, M Prencipe, ML Poeta, R Barbano, VM Valori, M Copetti, ... Cancer epidemiology, biomarkers & prevention 18 (10), 2694-2700 , 2009 2009 Citations: 103
A modified cell block technique for fine needle aspiration cytology. FA Zito, CD Gadaleta, C Salvatore, R Filotico, A Labriola, A Marzullo, ... Acta cytologica 39 (1), 93-99 , 1995 1995 Citations: 85
Conformation of bovine myelin basic protein purified with bound lipids E Polverini, A Fasano, F Zito, P Riccio, P Cavatorta European Biophysics Journal 28 (4), 351-355 , 1999 1999 Citations: 83
Comparison of formalin, ethanol, and Histochoice fixation on the PCR amplification from paraffin-embedded breast cancer tissue. C Giannella, FA Zito, F Colonna, A Paradiso, F Marzullo, M Alaibac, ... European journal of clinical chemistry and clinical biochemistry: journal of … , 1997 1997 Citations: 57
High concordance of BRAF status between primary colorectal tumours and related metastatic sites: implications for clinical practice D Santini, C Spoto, F Loupakis, B Vincenzi, N Silvestris, C Cremolini, ... Annals of oncology 21 (7), 1565 , 2010 2010 Citations: 56
Biology, prognosis and response to therapy of breast carcinomas according to HER2 score S Ménard, A Balsari, E Tagliabue, T Camerini, P Casalini, R Bufalino, ... Annals of oncology 19 (10), 1706-1712 , 2008 2008 Citations: 49
KRAS-driven lung adenocarcinoma and B cell infiltration: novel insights for immunotherapy P Rosamaria, P Daniela, L Rosanna, M Michele, C Annamaria, P Pamela, ... Cancers 11 (8), 1145 , 2019 2019 Citations: 45
Extraction and immobilization in one step of two β-glucosidases released from a yeast strain of Debaryomyces hansenii P Riccio, R Rossano, M Vinella, P Domizio, F Zito, F Sansevrino, A D’Elia, ... Enzyme and Microbial Technology 24 (3-4), 123-129 , 1999 1999 Citations: 43
Vascular endothelial growth factor concentrations from platelets correlate with tumor angiogenesis and grading in a spontaneous canine non-Hodgkin lymphoma model N Zizzo, R Patruno, FA Zito, AD Summa, A Tinelli, S Troilo, A Misino, ... Leukemia & lymphoma 51 (2), 291-296 , 2010 2010 Citations: 40
Quicktime virtual reality technology in light microscopy to support medical education in pathology FA Zito, F Marzullo, D D'Errico, C Salvatore, R Digirolamo, A Labriola, ... Modern Pathology 17 (6), 728-731 , 2004 2004 Citations: 40
NLRP3 inflammasome from bench to bedside: new perspectives for triple negative breast cancer M Sonnessa, A Cioffi, O Brunetti, N Silvestris, FA Zito, C Saponaro, ... Frontiers in oncology 10, 1587 , 2020 2020 Citations: 39
Ndg-cam: Nuclei detection in histopathology images with semantic segmentation networks and grad-cam N Altini, A Brunetti, E Puro, MG Taccogna, C Saponaro, FA Zito, ... Bioengineering 9 (9), 475 , 2022 2022 Citations: 37
Discordance between FISH, IHC, and NGS analysis of ALK status in advanced non–small cell lung cancer (NSCLC): a brief report of 7 cases A Scattone, A Catino, L Schirosi, L Caldarola, S Tommasi, R Lacalamita, ... Translational Oncology 12 (2), 389-395 , 2019 2019 Citations: 35
Ocular sarcoidosis: clinical experience and recent pathogenetic and therapeutic advancements R Dammacco, J Biswas, TT Kivelä, FA Zito, P Leone, A Mavilio, D Sisto, ... International Ophthalmology 40 (12), 3453-3467 , 2020 2020 Citations: 34
Prognostic value of NLRP3 inflammasome and TLR4 expression in breast cancer patients C Saponaro, E Scarpi, M Sonnessa, A Cioffi, F Buccino, F Giotta, ... Frontiers in oncology 11, 705331 , 2021 2021 Citations: 33
miRNA profiling in serum and tissue samples to assess noninvasive biomarkers for NSCLC clinical outcome D Petriella, S De Summa, R Lacalamita, D Galetta, A Catino, ... Tumor Biology 37 (4), 5503-5513 , 2016 2016 Citations: 33
Ovarian fibroma: our experience of 34 cases. G Gargano, M De Lena, F Zito, G Fanizza, V Mattioli, F Schittulli European journal of gynaecological oncology 24 (5), 429-432 , 2003 2003 Citations: 33
