Evaluating the mental health impact of PCOS: Limitations of the PSS-10 scale as stress validation tool Vidhu Gill, Kritanjali Singh, Kaynat Nasser Environment Conservation Journal, 2025 The present study intended to outline the mental health status of women with PCOS. A total of 150 women of reproductive age (with follow-up data available for 113 participants) presenting with polycystic ovarian syndrome (PCOS) and infertility (Type I and II) were recruited from Gynecology OPD. Baseline data prior to intervention revealed that 67.3% of participants, particularly those aged 20–30 years (mean = 22.4; SD = 5.98; p < 0.001; OR = 1.39 × 10⁷⁴), exhibited moderate levels of stress based on the Perceived Stress Scale-10 (PSS-10). A significant proportion (60.2%) had a healthy body mass index (BMI) (n = 68; OR = ∞; p < 0.001). Severe hirsutism (score 4) was rare (0.9%) but associated with elevated stress risk (estimate = 6758.4; OR = ∞). Marital status emerged as an additive factor contributing to stress (estimate = 7355.2; OR = ∞), while women from middle-income families were more vulnerable to stress (estimate = 51.1; OR = 1.54 × 10²²; p < 0.001).Demographic analysis indicated that a rural background did not significantly increase the risk of stress (estimate = –3088.8; OR = 0; p < 0.001). Working women had significantly higher PSS scores compared to housewives (Estimate = 4646.7; OR = ∞; p < 0.001). Although yoga was associated with reduced stress levels, incorrect posture and breathing techniques limited its effectiveness (estimate = 9567.8; p < 0.001), yet it still proved more beneficial than other forms of exercise. Notably, BMI showed an inverse correlation with stress levels, although this conflicted with qualitative data from personal interviews. Interviews further revealed that PCOS-related symptoms (e.g., bust size), marital status, and socio-economic factors influenced stress perception—elements not adequately captured by the PSS-10. Follow-up assessments demonstrated reduced stress scores post-intervention.In conclusion, while PSS-10 was useful in identifying short-term stress levels, it failed to capture the broader psychological burden experienced by women with PCOS and infertility in this study. These findings underscore the need for a more comprehensive, rather than a condition-specific, stress assessment tool to better evaluate mental health in women with reproductive disorders such as PCOS or infertility.
Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital Monika SHUKLA, Munish REDDY, Kritanjali SINGH, Ruchi SAINI, Pradeep RAGHAV, Kaynat NASSER, Nupur SHARMA, Aastha KAMRANI, Shehla RAFIQUE, Tanjula SHAIR Dental Press Journal of Orthodontics, 2024 Objective: To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP). Material and Methods: Sixty patients from the Orthodontics department of Subharti Dental College and Hospital (Meerut, India) were divided into two groups: Group 1 (Class II div. 1 malocclusion) and Group 2 (Class II div. 2 malocclusion). Then, 3 mL of blood was collected from each participant. DNA extraction was done, and Sanger Sequencing was performed from extracted DNA samples. Results: A statistically significant difference was found in the distribution of alleles among Class II div. 1 and Class II div. 2 malocclusions. The homozygous GG allele was the most prevalent among Class II div. 1 patients (76.7%), while the heterozygous AG allele was the most prevalent among Class II div. 2 patients (53.5%). Since GG was the most prevalent allele, it was used as a reference, and AA/AG were compared with GG to confirm the association. The results showed that individuals with the AG genotype seemed to be more susceptible to the development of skeletal Class II div. 2 malocclusion. Conclusion: The homozygous GG allele was the most prevalent among Class II div. 1 patients, while the heterozygous AG allele was the most prevalent among Class II div. 2 patients, suggesting that there could be a difference between the genetic association of both malocclusions.
Therapeutic advances for huntington’s disease Ashok Kumar, Vijay Kumar, Kritanjali Singh, Sukesh Kumar, You-Sam Kim, Yun-Mi Lee, Jong-Joo Kim Brain Sciences, 2020 Huntington’s disease (HD) is a progressive neurological disease that is inherited in an autosomal fashion. The cause of disease pathology is an expansion of cytosine-adenine-guanine (CAG) repeats within the huntingtin gene (HTT) on chromosome 4 (4p16.3), which codes the huntingtin protein (mHTT). The common symptoms of HD include motor and cognitive impairment of psychiatric functions. Patients exhibit a representative phenotype of involuntary movement (chorea) of limbs, impaired cognition, and severe psychiatric disturbances (mood swings, depression, and personality changes). A variety of symptomatic treatments (which target glutamate and dopamine pathways, caspases, inhibition of aggregation, mitochondrial dysfunction, transcriptional dysregulation, and fetal neural transplants, etc.) are available and some are in the pipeline. Advancement in novel therapeutic approaches include targeting the mutant huntingtin (mHTT) protein and the HTT gene. New gene editing techniques will reduce the CAG repeats. More appropriate and readily tractable treatment goals, coupled with advances in analytical tools will help to assess the clinical outcomes of HD treatments. This will not only improve the quality of life and life span of HD patients, but it will also provide a beneficial role in other inherited and neurological disorders. In this review, we aim to discuss current therapeutic research approaches and their possible uses for HD.
Advanced glycation end products (AGEs) may be a striking link between modern diet and health Vidhu Gill, Vijay Kumar, Kritanjali Singh, Ashok Kumar, Jong-Joo Kim Biomolecules, 2019 The Maillard reaction is a simple but ubiquitous reaction that occurs both in vivo and ex vivo during the cooking or processing of foods under high-temperature conditions, such as baking, frying, or grilling. Glycation of proteins is a post-translational modification that forms temporary adducts, which, on further crosslinking and rearrangement, form permanent residues known as advanced glycation end products (AGEs). Cooking at high temperature results in various food products having high levels of AGEs. This review underlines the basis of AGE formation and their corresponding deleterious effects on the body. Glycated Maillard products have a direct association with the pathophysiology of some metabolic diseases, such as diabetes mellitus type 2 (DM2), acute renal failure (ARF), Alzheimer’s disease, dental health, allergies, and polycystic ovary syndrome (PCOS). The most glycated and structurally abundant protein is collagen, which acts as a marker for diabetes and aging, where decreased levels indicate reduced skin elasticity. In diabetes, high levels of AGEs are associated with carotid thickening, ischemic heart disease, uremic cardiomyopathy, and kidney failure. AGEs also mimic hormones or regulate/modify their receptor mechanisms at the DNA level. In women, a high AGE diet directly correlates with high levels of androgens, anti-Müllerian hormone, insulin, and androstenedione, promoting ovarian dysfunction and/or infertility. Vitamin D3 is well-associated with the pathogenesis of PCOS and modulates steroidogenesis. It also exhibits a protective mechanism against the harmful effects of AGEs. This review elucidates and summarizes the processing of infant formula milk and the associated health hazards. Formulated according to the nutritional requirements of the newborn as a substitute for mother’s milk, formula milk is a rich source of primary adducts, such as carboxy-methyl lysine, which render an infant prone to inflammation, dementia, food allergies, and other diseases. We therefore recommend that understanding this post-translational modification is the key to unlocking the mechanisms and physiology of various metabolic syndromes.
Genetic heterogeneity of beta globin mutations among Asian-Indians and importance in genetic counselling and diagnosis Ravindra Kumar, Kritanjali Singh, Inusha Panigrahi, Sarita Agarwal Mediterranean Journal of Hematology and Infectious Diseases, 2013 There are an estimated 45 million carriers of β-thalassemia trait and about 12,000-15,000 infants with β-thalassemia major are born every year in India. The consanguinity rates are higher in India, and thalassemia major constitutes a significant burden on the health care system. In present study, β-thalassemia mutations were characterized in 300 thalassemia cases from 2007 to 2010 using ARMS-PCR and DNA sequencing. The five most common mutations accounted 79.3% of the studied chromosomes that includes IVS1-5(G>C), Cod 41-42(-TCTT), Cod8-9(+G), Cod16(-C) and 619bp del. Though IVS1-5(G>C) is most common mutation when all the communities were included, the percentage prevalence were calculated on sub caste basis and found that IVS1-5(G>C) percentage prevalence varied from 25 to 60 in Aroras & Khatris and Thakur respectively. Interestingly Cod41-42(-TCTT) mutation which is the second commonest among the mutations reported was totally absent in Kayasthas and Muslim community. These findings have implications for providing molecular diagnosis, genetic counseling and prenatal diagnosis to high risk couples of β-thalassemia.
An SP1-binding site polymorphism in the COLIAI gene: May be a strong predictor for low bone density in thalassemia major Gene Therapy and Molecular Biology, 2013
Status of 25-hydroxyvitamin D deficiency and effect of vitamin D receptor gene polymorphisms on bone mineral density in thalassemia patients of North India Kritanjali Singh, Ravindra Kumar, Anju Shukla, Shubha R. Phadke, Sarita Agarwal Hematology, 2012 Background Bone disease comprising of low bone mineral density (BMD), bone pain, and fractures is a characteristic feature of thalassemia. Vitamin D receptors (VDRs – FokI, TaqI, and Bsml) polymorphisms are closely related to low BMD at the lumbar spine and hips which can be used as a useful genetic marker in predicting bone disease in these patients. Aim To find out the status of VDRs gene polymorphisms and its effect on osteoporosis in thalassemia patients of North Indian origin. Material and methods BMD was measured in 40 beta-thalassemia major patients by dual-energy X-ray densitometry (DXA). Serum vitamin D levels were estimated by enzyme linked immunosorbant assay. VDR gene polymorphisms (FokI, TaqI, and BsmI) were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Results About 80.6% cases were found to be vitamin D deficient. Z score of BMD of lumbar spine and hips were −2.31 ± 1.18 and −2.09 ± 0.89. Osteoporotic lumbar spine was observed in 42.5% cases of thalassemia. A positive correlation of vitamin D level was found with Z score of BMD of lumbar spine (r = 0.398, P value = 0.027). Polymorphisms of FokI and BsmI were found significantly correlated with BMD of lumbar spine. However, no association of BMD was observed with TaqI polymorphism. Conclusion The present study showed a high prevalence of low BMD in thalassemia, suggesting that they should be targeted for DXA screening and osteoporosis prevention before permanent end organ bone damage occurs. The VDR genotyping can be used as additional test in individuals who are susceptible to osteoporosis so that early preventive measurements can be taken.
RECENT SCHOLAR PUBLICATIONS
Genetic association between class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital M Shukla, M Reddy, K Singh, R Saini, P Raghav, K Nasser, N Sharma, ... Dental press journal of orthodontics 29 (6), e2424128 , 2024 2024 Citations: 7
Non-syndromic Cleft Lip and Palate Patients of the North Indian Population and the Association of Methylenetetrahydrofolate Reductase Gene J Yadav, P Raghav, RK Mishra, DN Upadhyaya, V Prasad, CP Chaturvedi, ... Cureus 16 (7) , 2024 2024 Citations: 3
Association of ABO blood group and Rh factor in cleft lip and palate patients SK Yadav J, Raghav P, Misra RK, Prasad Veerendra International Journal of Research in medical Sciences 11 ((2)) , 2023 2023
“IS WAIST CIRCUMFERENCE PROVE TO BE A BETTER TOOL TO ASCERTAIN THE MAGNITUDE OF OBESITY" DSA Dr. Bhawana Pant ,Dr. Kaynat Nasser , Dr. Kritanjali Singh* International Journal of Applied Science 12 , 2022 2022
Troublesome state of mental stress of community during Covid-19 Pandemic: Is it a cause of concern for future perspective? DS Dave A, Nasser K, Singh K*, Pant B IJSERM , 2021 2021
Epidemiological Study on Mental Stress due to Covid19 among Community: An Online Cross Sectional Survey DSD Dr. Anuradha Dave1 , Dr. Kaynat Nasser2 , Dr. Kritanjali Singh3* , Dr ... International Journal of All Research Education and Scientific Methods … , 2021 2021
UBIQUITY OF VITAMIN D: A RETROSPECTIVE, HOSPITAL BASED STUDY KS Vidhu Gill Muzammil Bashir, Omar Naushad IJSR-International Journal of Scientific Research 10 (11), 53-55 , 2021 2021
ENDOPLASMIC RETICULUM STRESS AND METFORMIN: BOON OR BANE IN FEMALE COVID-19 PATIENTS VG Kritanjali Singh Neelu Khanna, Pawan Suri IJSR-International Journal of Scientific Research 10 (11), 29-31 , 2021 2021
Comparative study for better screening methods in blood bank of a tertiary care hospital for viral marker detection via rapid card or ELISA. KS Lalit Chaurasia. Akash Kumar, Davendra Swarup, Vidhu Gill IJSR-International Journal of Scientific Research 10 (10), 21-23 , 2021 2021
Neurobiology of zinc and its role in neurogenesis V Kumar, A Kumar, K Singh, K Avasthi, JJ Kim European Journal of Nutrition 60 (1), 55-64 , 2021 2021 Citations: 91
A Online Cross sectional study on reflection of mental stress K Singh IN Patent 18091/2021-CO/ L , 2021 2021
Estrogen Detoxification and Neuroendocrinological Activation and Balancing Via Hormonal Yoga KS Ashok Kumar Vidhu Gill Acta scientific neurology 3 (0), 106-111 , 2020 2020
Psycho-social trauma behind infertility: leading cause of biochemical stress among couples and yoga being effective therapeutic intervention BP Mamta Tyagi Vidhu Gill, Kritanjali Singh, Ashok Kumar International journal of scientific research 9 (6), 6-9 , 2020 2020
Therapeutic advances for Huntington’s disease A Kumar, V Kumar, K Singh, S Kumar, YS Kim, YM Lee, JJ Kim Brain sciences 10 (1), 43 , 2020 2020 Citations: 124
OSTEOPOROSIS IN THALASSEMIA MAJOR- REFLECTION IN INDIAN SCENARIO K SINGH SCHOLAR PRESS GERMANY , 2020 2020
Advanced glycation end products (AGEs) may be a striking link between modern diet and health V Gill, V Kumar, K Singh, A Kumar, JJ Kim Biomolecules 9 (12), 888 , 2019 2019 Citations: 264
questionnaire based proforma on polycystic ovarian syndrome and its associated disease V Gill, K Singh IN Patent 16088/2019-CO/ L , 2019 2019
A sequence variation: 713-8delC in the transforming growth factor beta 1 gene polymorphism in thalassemia major patients K Singh, S Agarwal, A Shukla, S Gupta Journal of Clinical Densitometry 17 (1), 185-189 , 2014 2014 Citations: 4
Low bone mineral density in thalassemia major: Sanjay Gandhi Post Graduate Institute experience and a brief focus on underlying factors behind the cause. K Singh, S Agarwal, S Gupta Thalassemia Reports 4 (1) , 2014 2014 Citations: 4
MOST CITED SCHOLAR PUBLICATIONS
Advanced glycation end products (AGEs) may be a striking link between modern diet and health V Gill, V Kumar, K Singh, A Kumar, JJ Kim Biomolecules 9 (12), 888 , 2019 2019 Citations: 264
Therapeutic advances for Huntington’s disease A Kumar, V Kumar, K Singh, S Kumar, YS Kim, YM Lee, JJ Kim Brain sciences 10 (1), 43 , 2020 2020 Citations: 124
Neurobiology of zinc and its role in neurogenesis V Kumar, A Kumar, K Singh, K Avasthi, JJ Kim European Journal of Nutrition 60 (1), 55-64 , 2021 2021 Citations: 91
Status of 25-hydroxyvitamin D deficiency and effect of vitamin D receptor gene polymorphisms on bone mineral density in thalassemia patients of North India K Singh, R Kumar, A Shukla, SR Phadke, S Agarwal Hematology 17 (5), 291-296 , 2012 2012 Citations: 66
Genetic heterogeneity of beta globin mutations among Asian-Indians and importance in genetic counselling and diagnosis R Kumar, K Singh, I Panigrahi, S Agarwal Mediterranean Journal of Hematology and Infectious Diseases 5 (1), e2013003 , 2013 2013 Citations: 21
Genetic association between class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital M Shukla, M Reddy, K Singh, R Saini, P Raghav, K Nasser, N Sharma, ... Dental press journal of orthodontics 29 (6), e2424128 , 2024 2024 Citations: 7
An SP1-binding site polymorphism in the COLIAI gene: may be a strong predictor for low bone density in thalassemia major K Singh, S Agarwal, S Gupta Gene Ther Mol Biol 15, 112-119 , 2013 2013 Citations: 7
A sequence variation: 713-8delC in the transforming growth factor beta 1 gene polymorphism in thalassemia major patients K Singh, S Agarwal, A Shukla, S Gupta Journal of Clinical Densitometry 17 (1), 185-189 , 2014 2014 Citations: 4
Low bone mineral density in thalassemia major: Sanjay Gandhi Post Graduate Institute experience and a brief focus on underlying factors behind the cause. K Singh, S Agarwal, S Gupta Thalassemia Reports 4 (1) , 2014 2014 Citations: 4
Non-syndromic Cleft Lip and Palate Patients of the North Indian Population and the Association of Methylenetetrahydrofolate Reductase Gene J Yadav, P Raghav, RK Mishra, DN Upadhyaya, V Prasad, CP Chaturvedi, ... Cureus 16 (7) , 2024 2024 Citations: 3
Association of ABO blood group and Rh factor in cleft lip and palate patients SK Yadav J, Raghav P, Misra RK, Prasad Veerendra International Journal of Research in medical Sciences 11 ((2)) , 2023 2023
“IS WAIST CIRCUMFERENCE PROVE TO BE A BETTER TOOL TO ASCERTAIN THE MAGNITUDE OF OBESITY" DSA Dr. Bhawana Pant ,Dr. Kaynat Nasser , Dr. Kritanjali Singh* International Journal of Applied Science 12 , 2022 2022
Troublesome state of mental stress of community during Covid-19 Pandemic: Is it a cause of concern for future perspective? DS Dave A, Nasser K, Singh K*, Pant B IJSERM , 2021 2021
Epidemiological Study on Mental Stress due to Covid19 among Community: An Online Cross Sectional Survey DSD Dr. Anuradha Dave1 , Dr. Kaynat Nasser2 , Dr. Kritanjali Singh3* , Dr ... International Journal of All Research Education and Scientific Methods … , 2021 2021
UBIQUITY OF VITAMIN D: A RETROSPECTIVE, HOSPITAL BASED STUDY KS Vidhu Gill Muzammil Bashir, Omar Naushad IJSR-International Journal of Scientific Research 10 (11), 53-55 , 2021 2021
ENDOPLASMIC RETICULUM STRESS AND METFORMIN: BOON OR BANE IN FEMALE COVID-19 PATIENTS VG Kritanjali Singh Neelu Khanna, Pawan Suri IJSR-International Journal of Scientific Research 10 (11), 29-31 , 2021 2021
Comparative study for better screening methods in blood bank of a tertiary care hospital for viral marker detection via rapid card or ELISA. KS Lalit Chaurasia. Akash Kumar, Davendra Swarup, Vidhu Gill IJSR-International Journal of Scientific Research 10 (10), 21-23 , 2021 2021
A Online Cross sectional study on reflection of mental stress K Singh IN Patent 18091/2021-CO/ L , 2021 2021
Estrogen Detoxification and Neuroendocrinological Activation and Balancing Via Hormonal Yoga KS Ashok Kumar Vidhu Gill Acta scientific neurology 3 (0), 106-111 , 2020 2020
Psycho-social trauma behind infertility: leading cause of biochemical stress among couples and yoga being effective therapeutic intervention BP Mamta Tyagi Vidhu Gill, Kritanjali Singh, Ashok Kumar International journal of scientific research 9 (6), 6-9 , 2020 2020
