Smita Kumbhar

Scopus Publications

Green biosynthesis and characterization of silver nanoparticles using Portulaca oleracea and evaluation of antioxidant activity
Mohini Salunke, Smita Kumbhar, Wakure Balaji
Journal of Research in Pharmacy, 2026
In the past several years, silver (Ag) nanoparticles (NPs) have made great progress because of their exceptional properties in a variety of applications. The green production method of nanoparticles has been gaining ground on conventional chemical and physical processes by eschewing the usage of costly devices and several harmful substances. Consequently, AgNPs have been produced in this study using Portulaca oleracea extract as reducing, capping, and stabilising agents. It is an easy, green, and environmentally friendly process. UV-visible spectroscopy, FTIR, XRD, and DLS were used to prove the stability and making of the synthesised Po-AgNPs. AgNPs production was demonstrated by the UV–vis spectra, which showed a transition from a colourless liquid to a brownish solution. FTIR (Fourier transform infrared spectroscopy) was used to show how the extract accessible functional groups contributed to the reduction and capping of NPs. The X-ray diffraction validated the synthesised NPs crystalline structure. The cumulant hydrodynamic diameter of Po-AgNPs was measured by DLS to be 368.6 nm, with a PdI of 0.433. The zeta potential value was found to be –34.3 mV, indicating that the Po-AgNPs are more stable and have negative charge. When biosynthesised Po-AgNPs were evaluated for antioxidant activity using DPPH tests, Po-AgNPs showed greater antioxidant activity. The results indicates that the stability, purity, degree of crystallinity, and cubic form of NPs produced by Portulaca oleracea extract are excellent. In general, more investigation is needed to examine the potential health benefits of the Ag-NP-based P. oleracea aqueous extract.
Houttuynia cordata–loaded nanophytosomes: Statistical optimization, structural characterization, and enhanced antioxidant performance
Smita Kumbhar, Somnath Bhinge, Manish Bhatia
Next Materials, 2026
Nanophytosomes are lipid-based nanocarrier systems designed to enhance the stability, bioavailability, and functional performance of plant-derived bioactives. In the present study, antioxidant-rich Houttuynia cordata extract was incorporated into phosphatidylcholine–cholesterol bilayers using the thin-film hydration method, followed by factorial design–based optimization. The optimized formulation (HC:PC:CH = 1:2:1.0) produced nanoscale vesicles with a mean particle size of 142.7 ± 2.4 nm , narrow size distribution (PDI: 0.214 ± 0.01), high encapsulation efficiency ( 83.0 ± 1.5% ), and a zeta potential of −32.6 ± 1.1 mV , indicating good colloidal stability. Fourier transform infrared spectroscopy confirmed non-covalent phytoconstituent–lipid interactions, while differential scanning calorimetry and X-ray diffraction analyses demonstrated suppression of crystallinity and molecular dispersion within the lipid matrix. Cholesterol concentration was identified as a key factor governing bilayer packing, membrane rigidity, and encapsulation performance. The optimized nanophytosomal formulation exhibited enhanced antioxidant activity compared to the free extract, with DPPH and ABTS IC₅₀ values of 21.68 ± 0.6 µg/mL and 18.94 ± 0.5 µg/mL , respectively. In vitro release studies revealed a biphasic release pattern with an initial burst followed by sustained release up to 24 h. Kinetic modeling indicated that drug release was best described by the Korsmeyer–Peppas model (R² = 0.978), suggesting a diffusion-controlled mechanism. The formulation demonstrated good physicochemical stability over 90 days under defined storage conditions. Overall, the study establishes a clear relationship between bilayer composition, nanoscale architecture, and functional performance, highlighting the potential of cholesterol-modulated nanophytosomes as effective carriers for enhancing the delivery of plant-derived antioxidants in nutraceutical and biomedical applications.
Regulatory integration of new approach methodologies for human-relevant developmental and reproductive toxicity (DART) assessment: A systematic cross-sector review
Smita Kumbhar, Shrikant Borude, Rahil Deshmukh
Toxicology Reports, 2026
Developmental and reproductive toxicity (DART) assessment is a critical component of regulatory safety evaluation across pharmaceutical, chemical, environmental, and consumer product sectors. Traditionally, DART testing has relied on animal-based studies designed to detect adverse effects on fertility, embryonic development, and postnatal growth. However, these conventional approaches present increasing scientific and ethical challenges, including high costs, extended study durations, limited mechanistic insight, and interspecies differences that may reduce their predictive value for human health outcomes. Concurrently, regulatory frameworks increasingly emphasize the principles of Replacement, Reduction, and Refinement (3Rs), driving the development of alternative testing strategies. This systematic review examines the evolving regulatory landscape for DART assessment and evaluates the scientific and regulatory progress associated with emerging New Approach Methodologies (NAMs). A structured literature search was conducted across major scientific databases following PRISMA 2020 guidelines, identifying relevant scientific and regulatory publications from January 2005 to February 2026. The review analyzes key international regulatory frameworks, including OECD Test Guidelines, ICH S5(R3), the European Union REACH regulation, and guidance from U.S. and European regulatory authorities . Particular emphasis is placed on mechanistic frameworks such as Adverse Outcome Pathways (AOPs) and Integrated Approaches to Testing and Assessment (IATA), which facilitate the integration of diverse data streams within weight-of-evidence–based regulatory decision-making. Overall, NAMs offer significant potential to enhance the efficiency, mechanistic understanding, and human relevance of DART assessment. Nevertheless, challenges related to method validation, regulatory harmonization, and integration into existing safety assessment frameworks remain, highlighting the need for continued scientific innovation and international regulatory collaboration.
Regulatory Complexities in MDSAP: Challenges and Pathways for Implantable Medical Device Approval
Smita Kumbhar, Shreya Khadtare, Snehal Zoman, Deepakshi Rane, Vipul Patel, Somnath Bhinge
Biomedical Materials and Devices, 2026
Ion-Pairing With Glycine Enhances Solubility, Dissolution, and Pharmacokinetics of Amlodipine Besylate: Physicochemical Characterization and In Vivo Evaluation
Smita Kumbhar, Prathamesh Kendale, Ravindra Jarag, Manish Bhatia
Chemistryselect, 2026
Poor aqueous solubility and variable oral bioavailability limit the therapeutic performance of amlodipine besylate. This study aimed to enhance its physicochemical and pharmacokinetic properties via ion‐pairing with glycine and evaluate its effects on solubility, dissolution, and in vivo performance. The ion‐pair complex was prepared by solvent evaporation under alkaline conditions. Characterization was performed using melting point, FTIR, and DSC analyses. Solubility was assessed at pH 1.2 and 7.4. Partition coefficient (log P ) was determined using an n ‐octanol/water system. Comparative dissolution studies were conducted, and pharmacokinetics were evaluated in Wistar rats. Acute toxicity was assessed as per OECD guideline 423. The complex showed significantly enhanced solubility (1.9‐fold at pH 1.2 and 3.1‐fold at pH 7.4; p < 0.05). DSC and FTIR confirmed molecular interaction and reduced crystallinity. Dissolution improved, with ∼1.7‐fold faster early release. A slight decrease in log P indicated increased hydrophilicity without compromising permeability. C max and AUC increased, while T max remained unchanged (p > 0.05). LD50 increased, indicating improved safety. Ion‐pairing with glycine improves solubility, dissolution, and bioavailability of amlodipine besylate without compromising permeability or safety, offering a simple and scalable strategy for poorly soluble drugs.
Smart UDI for Medical Devices: Global Harmonization, Cybersecurity, and Digital Convergence
Smita Kumbhar, Alfej Tamboli
Biomedical Materials and Devices, 2026
Integrating artificial intelligence for design, optimization and pharmacokinetic prediction in nanoparticle based drug delivery
Hariharan Thirumalai Vengateswaran, Mohammad Habeeb, Rehan Ahmed, Huay Woon You, Smita Tukaram Kumbhar, K.N.V. Chenchu Lakshmi, Prasad Laxman Gorde
Journal of Drug Delivery Science and Technology, 2026
Formulation of folic acid-decorated β-cyclodextrin-based magnetic nanoparticles hybrid nanotherapeutic for targeted delivery of chlorogenic acid
Shagufta Juber Pathan, Somnath Devidas Bhinge, Shrinivas Krishna Mohite, Sopan Namdev Nangare, Mangesh Anil Bhutkar, Namdeo Ramhari Jadhav, Shubhangi Patil, Smita Kumbhar, Shailendra Shivaji Gurav
Journal of Drug Delivery Science and Technology, 2025
An Innovative Dual-Drug Topical Hydrogel for Synergistic Wound Healing: Formulation and Evaluation
Neela Bhatia, Smita Kumbhar, Anagha Ajagekar, Rutuja Chougale, Manish Bhatia
Journal of Pharmaceutical Innovation, 2025
Wound healing Potential of Fucoidan Extracted Microwavically from Sargassum wightii Possibly Mediated by Collagen-1 Expression in Vero Cell Line
Smita Kumbhar, Shubhangi Birajdar, Manish Bhatia
Jordan Journal of Pharmaceutical Sciences, 2025
Background: Fucoidan, a natural macromolecule extracted from Sargassum wightii, has shown promise in various therapeutic areas, including anti-tumor, antioxidant, antithrombotic, and wound healing applications. This study explores the wound healing potential of fucoidan derived from Sargassum wightii collected from the Gulf of Mannar, Tamil Nadu, India. Aims and Objectives: This research aims to assess the effectiveness of fucoidan extracted via microwave-assisted extraction (MAE) in promoting wound healing in Vero cells, a line of African green monkey kidney cells. The study also investigates the impact of fucoidan on collagen-1 expression, a critical protein involved in the wound healing process. Materials and Methods: Fucoidan was extracted using MAE, and its cytotoxicity was evaluated using the Sulforhodamine B (SRB) Assay. The wound healing efficacy was tested through a scratch assay, measuring the closure of wounds over 24 and 48 hours. Results: The SRB Assay demonstrated that fucoidan did not exhibit cytotoxicity to Vero cells, with an IC50 value of 61.30 µM. The scratch assay revealed wound closure of 46.15% at 24 hours and 76.9% at 48 hours, compared to 50% and 81.25% in the control group. Fucoidan treatment significantly increased collagen-1 expression, with 77.92% of cells showing elevated levels of this crucial protein. Conclusions: This study confirms the in-vitro wound healing capabilities of fucoidan extracted from Sargassum wightii. These findings support the potential of fucoidan as a natural agent for wound healing and restoration.
HR-LCMS and FTIR characterization of bioactive components of Turbinaria ornata
Mohini Salunke, Preeti Mane, Smita Kumbhar, Balaji Wakure
Research Journal of Pharmacy and Technology, 2025
Nanostructured Phytosomes: Revolutionizing Herbal Compound Delivery, Therapeutic Applications, Current Achievements and Future Perspectives
Smita Kumbhar, Mohini Salunke, Balaji Wakure
Research Journal of Pharmacy and Technology, 2025
Advances in Lipid-Based Nanomedicine: Pathway Specific siRNA Therapy and Optimizing Delivery for Hepatocellular Carcinoma
Karkaz Thalij, Huay Woon You, Kiran Aher, Girija Bhavar, Smita Kumbhar, Mohammad Habeeb
International Journal of Nanomedicine, 2025
Fucoxanthin Shields Vero Cells from H2O2-Induced Oxidative Damage by Potentially Activating the Nrf2 Signalling Pathway
Smita Kumbhar, Manish Bhatia, Prafulla Choudhari, Vinod Gaikwad, Mohini Salunke, Balaji Wakure
Research Journal of Pharmacy and Technology, 2025
Biowaiver based on biopharmaceutics classification system: Considerations and requirements
Pratik R. Dhake, Smita T. Kumbhar, Vinod L. Gaikwad
Pharmaceutical Science Advances, 2024
Enhancing biomedical imaging: the role of nanoparticle-based contrast agents
Mohammad Habeeb, Hariharan Thirumalai Vengateswaran, Arpan Kumar Tripathi, Smita Tukaram Kumbhar, Huay Woon You, Hariyadi
Biomedical Microdevices, 2024
Advancements and best practices in diabetic foot Care: A comprehensive review of global progress
Smita Kumbhar, Manish Bhatia
Diabetes Research and Clinical Practice, 2024
Targeting Cancer with Sweet Precision: Saccharide-Grafted Erlotinib for Enhanced Drug Delivery
Isha Shete, Smita Kumbhar, Rakesh Dhavale, Manish Bhatia
Journal of Pharmaceutical Innovation, 2024
An evaluation of Sargassum cinctum anticancer properties utilizing in vitro testing and molecular docking, with assistance from GC-HRMS and FTIR
Mohini Salunke, Preeti Mane, Smita Kumbhar, Balaji Wakure
Journal of Applied Pharmaceutical Science, 2024
Targeted nanomedicine modulating intercellular communications to arrest renal cell carcinoma progression
Mohammad Habeeb, Shivani Arsey, Huay Woon You, Smita Tukaram Kumbhar, Kiran Balasaheb Aher, Girija Balasaheb Bhavar, Hariharan Thirumalai Vengateswaran
Journal of Drug Delivery Science and Technology, 2024
The Development of an Innovative Ophthalmic In-situ Gel Containing Posaconazole
Kumbhar ST, Salunke MA, Mane PT, Wakure BS
International Journal of Drug Delivery Technology, 2024
Phytosomes: A Cutting-Edge Platform for Phytochemicals Delivery by Enhancing Bioavailability
Smita Kumbhar, Vaishnavi Jagdale, Manish Bhatia
Jordan Journal of Pharmaceutical Sciences, 2024
Unleashing the Power of Rutin-Loaded Nanophytosomes: Enhancing Antioxidant Potential for Improved Health Outcomes
Smita Kumbhar, Nitin Patil, Bhausaheb Patil, Huay Woon You, Manish Bhatia
World Journal of Environmental Biosciences, 2024
Nano-bio interactions: understanding their dynamic connections
Mohammad Habeeb, Hariharan Thirumalai Vengateswaran, Smita Tukaram Kumbhar, Huay Woon You, Govind Sarangdhar Asane, Kiran Balasaheb Aher
Theranostics Nanomaterials in Drug Delivery, 2024
Evaluation of curcumin-loaded chitosan nanoparticles for wound healing activity
Smita Kumbhar, Rupali Khairate, Manish Bhatia, Prafulla Choudhari, Vinod Gaikwad
Admet and Dmpk, 2023
Development of Novel RP-HPLC Method for Estimating Tepotinib in Bulk and Pharmaceutical Dosage Form
Smita T Kumbhar, Pratima S Kokare, Pradip B Digge
International Journal of Pharmaceutical Quality Assurance, 2023
Synthesis, Characterization, In Silico Analysis, and Pharmacological Evaluation of Metoprolol-Modified Saccharide Conjugates for Cardiovascular Targeting
Smita Tukaram Kumbhar, Shitalkumar Shivgonda Patil, Manish Sudesh Bhatia
Journal of Pharmaceutical Innovation, 2022
Synthesis andcharacterization of chitosan nanoparticles decorated with folate and loaded with dasatinib for targeting folate receptors in cancer cells
Smita Tukaram Kumbhar, Ravikant Yashwantrao Patil, Manish Sudesh Bhatia, Prafulla Balkrushna Choudhari, Vinod Limbraj Gaikwad
Opennano, 2022
ENGINEERED ATENOLOL-GLYCOCONJUGATES TO TARGET H9C2 CARDIOMYOCYTE CELL LINES
Smita Tukaram Kumbha, Manish Sudesh Bhatia, Shitalkumar Shivgonda Patil
Acta Innovations, 2022
In silico design and pharmacological evaluation of conjugates of atenolol with modified saccharide for cardiovascular targeting
Smita Tukaram Kumbhar, Shitalkumar Shivgonda Patil, Manish Sudesh Bhatia
Glycoconjugate Journal, 2021
A review on basics and applications of modified carbohydrates in drug delivery
Indian Drugs, 2021
Formulation and evaluation of lignocaine hydrochloride topical gel
Smita Kumbhar, Vinod Matole, Yogesh Thorat, Saili Madur, Smeeta Patil, Anita Shegaonkar
Research Journal of Pharmacy and Technology, 2021
Optimization of thiazolidone scaffolds using pocket modeling for development of potential secretory system inhibitors of mycobacterium tuberculosis
Shivratna V. KHARE, Sujata P. CHOUDHARI, Siddharth P. PHALLE, Santosh S. KUMBHAR, Prafulla B. CHOUDHARI, Sambhaji R. MASAL, Aakash K. PATIL, Rakesh P. DHAVALE, Durgacharan A. BHAGWAT, Atul M. KADAM
Turkish Journal of Pharmaceutical Sciences, 2019
Investigation of QSAR kNN-MFA on a series of substituted chromen-2-one derivatives as FXa inhibitors
Jordan Journal of Pharmaceutical Sciences, 2018
Synthesis, antimycobacterial screening and molecular docking studies of 4-aryl-4′-methyl-2′-aryl-2,5′-bisthiazole derivatives
Yogita K. Abhale, Abhijit D. Shinde, Keshav K. Deshmukh, Laxman Nawale, Dhiman Sarkar, Prafulla B. Choudhari, Santosh S. Kumbhar, Pravin C. Mhaske
Medicinal Chemistry Research, 2017
Visible spectrophotometric method for estimation of bisoprolol from its bulk and tablet formulation
Asian Journal of Pharmaceutical and Clinical Research, 2013
Design and evaluation of floating tablets of diltiazem hydrochloride
International Journal of Pharma and Bio Sciences, 2012
Development and validation of derivative spectrophotometric method for estimation of atorvastatin calcium and amlodipine besylate in tablet dosage form
International Journal of Pharmacy and Pharmaceutical Sciences, 2011

Smita Kumbhar

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Scopus Publications