Agricultural and Biological Sciences, General Agricultural and Biological Sciences, Agricultural and Biological Sciences
12
Scopus Publications
Scopus Publications
INFLUENCE OF POLISHING DEGREE ON TECHNOLOGICAL TRAITS OF GRAIN OF DIFFERENT RICE VARIETIES Elina Yu. Papulova, Natalya G. Tumanyan, Svetlana S. Chizhikova, Ruslan N. Troyan, Tatyana B. Kumeiko Siberian Journal of Life Sciences and Agriculture, 2025 Background.The quality of rice products, their physical properties and nutritional value depend on the degree of grain processing, including its polishing, which removes the surface shells and the germ. The task of the work is to assess the effect of the degree of grain polishing of Russian-bred rice varieties with different grain sizes and shapes on the physical (technological) traits of the grain.Purpose.The goal of the study was to assess the influence of the grain polishing degree of Russian-bred rice varieties with different grain sizes and shapes on the physical (technological) traits of the grain.Materials and methods.The research material included the following varieties: Favorit, Veles, Trio, Regul 2, Forsazh, Strombus, Prestige, Vector, most of which are approved for use. The set of parameters for assessing grain quality included the following indicators: grain size, vitreousity, grain fracturing, total milling yield, head rice content, and bran yield during polishing. The duration of polishing was 50, 90, and 120 seconds.Results.The mass of 1000 absolutely dry grains was in the range of 25.6–26.6 g in the group of medium-weight varieties and 28.4–33.1 g in the group of large-grain rice varieties. In the group of large-grain varieties, the varieties Forsazh and Strombus were the most resistant to intensive polishing, the varieties Prestige and Regul 2 were the least resistant; in the group of medium-weight varieties, the corresponding indicators of the head rice content were significantly lower.Conclusion.Significant dynamics of the indicators was noted already at the initial stage of grain polishing (90 s) in the rice varieties Favorit and Strombus for the indicator “head rice content”, Favorit and Utes - for the bran yield; at the stage of 120 s - in the vari-eties Regul 2 and Vector, Prestige for all traits, the varieties Trio and Forsazh for the indicators “total milling yield” and “bran yield”. Equivalent changes in the indicator of traits with different degrees of polishing were typical for the varieties Strombus and Utes for the indicator of total milling yield; Trio, Utes and Forsazh- for the head rice content and the variety Strombus - for the bran yield.
EVALUATION OF QUALITY OF EXPERIMENTAL WHITE-GRAN RICE POPULATIONS IN ACCELERATED BREEDING BY GRAIN SIZE AND VITREOUSITY Natalya G. Tumanyan, Elina Yu. Papulova, Liana M. Lalayan, Svetlana S. Chizhikova, Tatyana B. Kumeiko Siberian Journal of Life Sciences and Agriculture, 2025 Background.In marker-assisted rice breeding, the accelerated development of varieties with superior grain quality traits through advanced biotechnological approaches requires the generation of segregating rice populations followed by phenotyping of genotypes for traits of interest. These segregating populations are used to identify genetic loci (QTLs) associated with complex traits, including rice grain quality, based on phenotypic data.Purpose.The goal of the work was to evaluate experimental BC3populations of rice based on physical characteristics of grain: size, vitreousity, fracturing, in order to carry out work on targeted selection based on phenotyping and genotyping data of promising plants - prototypes of varieties with specified traits in marker-assisted rice breeding.Materials and methods.The study involved hybrids of 15 combinations of parental forms. The seeds were sown in vessels on the vegetation site of FSBSI Federal Scientific Rice Centre, Pryanishnikov’s mixture was used as the main fertilizer; as they ripened, the seeds were harvested manually. High-tech methods of phenotyping the breeding material were used to conduct the research. The grain size was estimated by the mass of 1000 absolutely dry grains using a moisture analyzer, an air-heat unit, and an automatic seed counter; the vitreousity and grain fracturing were estimated in transmitted light using a diaphanoscope.Results.Genotypes were differentiated and distributed into groups for each trait. As a result of the quality study of the obtained BC3samples, lines combining high technological grain quality traits were identified using phenotyping data. The mass of 1000 absolutely dry grains was in the range of 23.2-30.2 g in the group of medium-weight samples, the indices of vitreousity and fracturing were 62-93% and 1-9%, respectively.Conclusion.As a result of the comparative analysis of hybrids and parental forms, combinations were noted for which the heterosis effect was typical for grain quality traits.
Effective protocol for rice phenotyping based on grain quality: ranking of rice samples from a segregating BC2 population Zhanna Mukhina, Liubov Yesaulova, Natalya Tumanyan, Elina Papulova, Sergei Garkusha, Nikolay Chukhir E3s Web of Conferences, 2024 The physical properties of rice backcross samples were studied in connection with the development of an effective breeding methodology for creating rice genotypes based on an accelerated breeding process. The purpose of the research was to study the traits of size, vitreousity, and fracturing of samples of the segregating population of BC2 reseedings from the 2022 and 2023 yield. As part of the program for developing varieties based on the use of modern post-genomic and cellular technologies, BC2 samples were used; as parent forms – samples of the USI “Collection of Federal Scientific Rice Centre” of various groups according to the shape of the grain. The crop was grown in artificial climate chambers under controlled conditions of light and air temperature and on the growing site of Federal Scientific Rice Centre. In the study, white grain samples from the segregating BC2 population were isolated for large-scale genotyping. The cultivation of parental forms for hybridization and the crossing process were carried out in artificial climate chambers. Hybridizations were performed using pneumocastration, which included cutting off the flower scales by 1/3 and then removing the anthers using a vacuum compressor. White grain BC2 samples were isolated for research. Seeds were sown on the growing area in containers with 6 kg of soil and Pryanishnikov’s mixture as the main fertilizer. After ripening, the seeds were manually harvested. Plant phenotyping of experimental populations was carried out basing on grain quality traits using certified equipment in accordance with the instructions for the devices and GOST standards. For 15 combinations, samples with the best characteristics of grain quality were selected. In connection with the indicators of physical grain quality traits, BC2 samples were classified into three groups with indicators for each trait: high, medium, low.
Assesment of rice yield quality in Kuban in connection with grain damage in the form of dark spots Natalia Tumanyan, Tatiana Kumeyko, Svetlana Chizhikova, Elina Papulova Bio Web of Conferences, 2024 The problem of rice damage by “black spotting” in the field during maturation is acute in all rice-growing countries. In the work, it was necessary to study the spread of shield bugs on rice crops in the Krasnodar region, the intensity of damage to rice crops in various areas according to the method of assessing damage to the rice crop, differentiation of varieties by tolerance to damage. Surveys of rice crops were carried out during various periods of the growing season in order to identify insect pests with piercing-sucking mouthparts in the Krasnoarmeysky, Abinsky, Slavyansky, Kalininsky districts of Krasnodar region and Prikubansky district of Krasnodar in 2020-2023 and a comparative assessment of the intensity of damage to rice grains in the form of dark spots by region is given. The highest intensity of grain damage was noted in Krasnoarmeysky district. Preliminary results on the tolerance of varieties to grain damage by stink bugs during the rice growing season were obtained and preliminary differentiation of varieties was carried out. The most tolerant varieties were Abinsky, Vector, and Strombus, and therefore these varieties are recommended to be grown in areas with the most intense damage to crops by stink bugs. Methodological methods have been developed for studying the “black spotting” of rice, which consist of selecting samples of rice varieties in industrial crops in areas of its cultivation, characterizing the content of damaged grains and assessing the influence of the intensity of damage on the physical properties of the yield.
Phenotyping of experimental rice population in accelerated breeding of new varieties with valuable grain quality traits Zhanna Mukhina, Natalya Tumanyan, Ellina Papulova, Liubov Yesaulova, Sergei Garkusha Bio Web of Conferences, 2024 The study of the consumer advantages of rice samples was carried out in connection with the development of an effective breeding methodology for developing rice genotypes based on an accelerated breeding process. The aim of the research was to study the protein and amylose content in rice grain samples of the parent white-grain and red-grain forms and generations of BC2. In the program of developing varieties based on the use of modern postgenomic and cellular technologies, BC2 and their parent forms - samples of the USI “Collection of the FSC of rice” belonging to various groups by the grain form, white-grained, served as the research material. The crop was grown on the experimental plot of Federal Scientific Rice Centre. As a result of the work carried out, samples with an unpainted pericarp were isolated for large-scale genotyping. Phenotyping of the studied rice samples was carried out by protein and amylose content in rice grains. The assessment on the basis of “protein content” was carried out using a universal infrared express analyzer “Multicheck Plus”, InfraLUM FT 6, the amylose content in starch of rice grain varieties – according to GOST ISO 6647-2-2015 Fig. BC2 groups with a high protein content, more than 8.8% and amylose content 1 – 16.0-19.0 %, 2 - amylose content 20.0-22.0%, 3 - amylose content 22.1-27.0% were identified in BC2 grain combinations.
IMPROVING COLOURED RICE GRAIN QUALITY THROUGH ACCELERATED BREEDING ZHM MUKHINA Sabrao Journal of Breeding and Genetics, 2024 ZH.M. MUKHINA, N.G. TUMANYAN, S.V. GARKUSHA, E.YU. PAPULOVA, N.P. CHUKHIR, I.N. CHUKHIR, E.YU. GNENNIY, L.V. ESAULOVA, E.A. MALYUCHENKO, and N.I. VAKHRUSHEVA Citation: Mukhina ZhM, Tumanyan NG, Garkusha SV, Papulova EYu, Chukhir NP, Chukhir IN, Gnenniy EYu, Esaulova LV, Malyuchenko EA, Vakhrusheva NI (2024). Improving coloured rice grain quality through accelerated
RICE PHENOTYPING THROUGH GRAIN QUALITY TRAITS FOR THE DEVELOPMENT OF NEW GENERATION CULTIVARS ZH.M. MUKHINA, N.G. TUMANYAN, E.YU. PAPULOVA, E.YU. GNENNIY Sabrao Journal of Breeding and Genetics, 2022 The present rice market requires a range of rice products with increased eating and nutritional values. The presented work was carried out within the framework of the breeding program for developing new rice cultivars with desirable grain quality traits based on an integrated approach, using modern methods of trait phenotyping and the genomic approach, post genomic, and cellular technologies. The latest study aimed to phenotype rice cultivars from the Unique Scientific Installation (USI) - Collection of Federal Scientific Rice Centre, Krasnodar, Russia, as sources of valuable grain quality traits. Studied traits included vitreosity, fracturing, and protein and grain amylose content. The coefficient of variation determined the variability of traits and helped identify the best cultivars as sources of valuable traits. The grain quality of harvested sample cultivars showed significant differences. The rice cultivar Thaibonnet gave the best results in terms of grain quality traits and variability. By combining low variability of technologically-tested quality traits and protein content, the rice cultivars Elbrus and Thaibonnet led all the samples. Cultivar Svetlana was characterized by high traits of vitreosity and fracturing and having less variability. The cultivars Leader and Patriot both had high protein content and low trait variability. These cultivars are recommended for use in parental crosses in breeding programs to develop rice genotypes with desirable grain quality traits and nutritional values.
Physical and chemical properties of rice grain of variety Rapan at different seeding rates and does of nitrogen fertilizers Elina Papulova, Svetlana Chizhikova, Knarik Olkhovaya, Natalia Tumanyan E3s Web of Conferences, 2021 The paper presents the results of evaluating the rice variety Rapan grown in 2018, 2019 at different seeding rates and doses of nitrogen fertilizers, according to the main amylographic characteristics of starch dispersion and fracturing. In 2018 and 2019, when growing variety Rapan with seeding rates of 4, 6 and 8 million viable seeds, the highest values were noted for the “fracturing” trait at a nitrogen dose of 92 kg/ha of active ingredient. In 2018, they amounted to 31, 35 and 41%, respectively, in 2019 - 13, 12 and 12%, respectively. The following tendency in the change of rice quality traits was noted: in the samples grown without the use of nitrogen, low fracturing was revealed, while in the N92 variants the value of the trait was maximum, with N184 - the minimum. Indicators for the “maximum viscosity” at a nitrogen dose of 92 kg / ha of active ingredient had the lowest values at all three seeding rates and amounted to 484, 500 and 485 Br.units in 2018, respectively. In 2019 - 471, 440 and 480 Br. units. The opposite pattern was observed when determining the viscosity indices: at the minimum values of fracturing, the viscosity indices were maximum and decreased at high values of this trait.
Impact of degree of polishing on technological and biochemical grain quality traits of rice varieties of Russian breeding N G Tumanian, E Y Papulova, S S Chizhikova, T B Kumeiko Iop Conference Series Earth and Environmental Science, 2021 The article presents the results of evaluation of rice varieties grown in the conditions of Krasnodar region by technological and biochemical quality traits in connection with the degree of grain polishing. The experiments were carried out in the laboratory of grain quality of Federal Scientific Rice Centre, Krasnodar. Grain of four rice varieties of Russian breeding (Rapan, Flagman, Polevik and Olimp) was subjected to five different degrees of polishing (0 %, 10 %, 12 % and 14 %). The degree of polishing significantly (P≤0.05) influenced the total yield of polished rice, head rice content, and the content of protein and amylose. An increase in the degree of polishing led to a further decrease in the protein content, total yield of polished rice and head rice content in it. However, an increase in the amylose content was found for simulated samples. With an increase in the degree of polishing to 14%, the amylose content increased in varieties Rapan, Flagman, Polevik and Olimp, respectively, by 1.3 %, 1.4 %, 1.5 % and 1.2 %, the protein content decreased by 1.5 %, 1.7 %, 1.6 %, 1.7 %.
Colored rice varieties of Russian breeding in terms of grain quality for development of functional rice varieties N G Tumanian, Zh M Mukhina, L V Esaulova, E Yu Papulova, E G Savenko, S V Garkusha E3s Web of Conferences, 2020 In order to develop rice breeding material of a functional direction, technological and biochemical grain quality traits of varieties bred by the Federal Scientific Rice Centre (Rubin, Mars, Mavr, Gagat, Yuzhnaya noch) with a colored grain pericarp, high content of anthocyanins and intended for functional nutrition were studied. The varieties were grown in the valley agrolandscape zone of Krasnodar region (Russia) in 2017–2019. The studied rice varieties are included in the State Register of Protected Breeding Achievements. The grain size by weight of 1000 absolutely dry grains (weight of 1000 a.d.g.) was determined according to GOST 10842-89, fracturing on a DSZ-3 diaphanoscope, protein content was measured using an Infralum FT-10 device. The determination of amylose was carried out colorimetrically using the amylose-iodine reaction according to Juliano. The varieties have a medium-sized caryopsis (21.5-27.0 g of 1000 grains); of them, the variety Yuzhnaya noch is characterized by the smallest grain. The variety Mars belongs to the low amylose group, varieties Mavr, Rubin and Gagat to the medium amylose group, Yuzhnaya noch to the waxy group. In terms of protein content in grain, all varieties are classified as medium protein. The maximum grain fracture was in variety Mavr in the range of 19-25% and the minimum in Mars and Gagat, respectively 2-5 and 2-6%. Variations in grain size indicators, amylose and protein content of varieties are weak in all varieties of special purpose, which testified to their high stability under growing conditions.
