Biotechnology, Biochemistry, Genetics and Molecular Biology, Applied Microbiology and Biotechnology, Chemical Engineering
26
Scopus Publications
1657
Scholar Citations
17
Scholar h-index
22
Scholar i10-index
Scopus Publications
Structural and Functional Characterization of Type IV Pilus-Associated Proteins PilV, Pil94, and Pil96 of Aeromonas hydrophila: Potential Role in Human Pathogenesis Agradip Bhattacharyya, Goutam Banerjee, Pritam Chattopadhyay Current Pharmaceutical Biotechnology, 2026 OBJECTIVE: Recent genomic analyses identified a distinct pilVWXY operon in hypervirulent Aeromonas hydrophila ATCC 7966, encoding PilV and two previously uncharacterized orthologs, AHA_0694 (Pil94) and AHA_0695 (Pil96), hypothesized to mediate host-pathogen interactions. METHODS: Complete A. hydrophila genomes (n = 53) were retrieved from NCBI, and the distribution of pilV, pil94, and pil96 was assessed using STRING v11.5. Physicochemical and structural features were analyzed via ExPASy-ProtParam, homology modeling, and validation using RAMPAGE, ProQ, and ProSA. Representative models were docked with eight human β- integrins using the HADDOCK server and evaluated by HADDOCK score, cluster size, van der Waals energy, RMSD (root-mean-square deviation), buried surface area, and Z-score. RESULTS: Twenty-eight strains of A. hydrophila (predominantly hypervirulent) encoded all three proteins, whereas 11 non-virulent strains lacked them. Structural modelling revealed a conserved lollipop-like conformation with an extended N-terminal α-helix characteristic of Type IV pilins. Docking simulations indicated selective, high-affinity binding patterns (PilV with Integrin β3/β4/β7; Pil94 with integrin β1/β2/β3/β5; Pil96 with integrin β1/β3/β5/β7/β8), suggesting roles in multi-tissue adhesion and systemic dissemination. CONCLUSION: The restricted occurrence of pilV, pil94, and pil96 in virulent strains and their predicted affinity for human β-integrins underscore their importance in host colonization and pathogenesis, identifying them as promising molecular targets for diagnostic or therapeutic development.
Diversity and Distribution of Non-Reducing Polyketide Synthases (NR-PKSs) in Ascomycota (Fungi) Pritam Chattopadhyay, Goutam Banerjee Journal of Fungi, 2025 (1) Background: This study highlights the diversity and distribution of non-reducing polyketide synthases (NR-PKSs) in Ascomycota and their role in producing bioactive aromatic polyketides. (2) Methods: A reference dataset of non-NR-PKSs was compiled from published literature and cross-examined using NaPDoS2 and Kyoto Encyclopedia of Genes and Genomes Ortholog (KEGG KO) databases. Signature domains were validated through Pfam and CDD, while phylogenetic classification was conducted by comparing the dataset with the NaPDoS2 reference tree. Cluster support was derived from KEGG KO and homology-based modeling. Additionally, NR-PKS clade distribution across KEGG genomes was analyzed, and co-expression patterns were examined using STRING. (3) Results: This study identified nine distinct clades of NR-PKSs, six of which are supported by unique KEGG Orthology (KO) numbers. These clades are as follows: clade 1: polyketide synthase A (PksA, K15316); clade 2: fusarubinsynthase 1 (Fsr1); clade 3: white A (WA, K15321); clade 4: polyketide synthase citrinin (PksCT); clade 5: zearalenone synthase 1 (Zea1, K15417); clade 6: orsellinic acid synthase A (OrsA, K15416); clade 7: aurofusarin polyketide synthase A (AptA, K15317); clade 8: monodictyphenone polyketide synthase G (MdpG, K15415); and clade 9: bikaverin polyketide synthase (Bik1). The present investigation also reports incongruency in the distribution of different NR-PKSs and fungi phylogeny within the phylum Ascomycota. (4) Conclusions: The distribution of NR-PKSs in Ascomycota defies phylogenetic boundaries, reflecting the impact of horizontal gene transfer, gene loss, and ecological adaptation.
Investigating the Distribution of Intimin and Invasin in Aeromonas hydrophila and Their Role in Host Tissue Attachment Agradip Bhattacharyya, Goutam Banerjee, Pritam Chattopadhyay Bacteria, 2025 Background: Aeromonas hydrophila is a key pathogen affecting freshwater fish, including Labeo rohita (rohu), causing significant aquaculture losses. This study explores the role of intimin and invasin, known virulence factors, in A. hydrophila pathogenesis using in silico methods. Methods: We analyzed the distribution of invasin and intimin across 53 A. hydrophila genomes and examined their physicochemical properties, secondary structures, and 3D models. Since crystal structures were unavailable, homology-based modeling was employed to study the structure of rohu β-integrins. In silico docking was performed to explore the interactions between intimin/invasin and β-integrins. Results: Our findings revealed that intimin and invasin were present in only 6 out of the 53 A. hydrophila strains examined, which were designated as hypervirulent strains. The transmembrane regions of intimin and invasin were modeled as β-barrels, a common feature of porins. The in silico docking experiments indicated the significant binding affinity of invasin and intimin with all the β-integrins of rohu fish, suggesting a critical role in host attachment and cellular internalization. Conclusions: This in silico study highlights the pivotal role of invasin and intimin in host tissue’s binding efficacy, offering valuable insights into the binding potential of A. hydrophila across various organs in rohu fish.
Probable Role of Type IV Pili of Aeromonas hydrophila in Human Pathogenicity Agradip Bhattacharyya, Goutam Banerjee, Pritam Chattopadhyay Pathogens, 2024 Background: Aeromonas hydrophila is a widely recognized broad-spectrum pathogen that primarily targets the gastrointestinal tract. Type IV pili (T4P) are proteinaceous nano-machines located on the bacterial cell surface, playing a crucial role in host colonization and infection. Regrettably, the T4P systems of A. hydrophila remain largely underexplored. Methods: A. hydrophila genomes with complete genome assembly and annotation reports up to 31 March 2023, were obtained from the NCBI Genome database or KEGG genome database, followed by a global search for T4P secretion system genes. Protein sequences of these manually curetted genes were used as secondary quarry for Synteny analysis. Protein–protein interaction analysis was performed by string analysis and in silico study of genomic islands. Results: We identified 27 orthologs of type IV pili (T4P) nano-machine components in A. hydrophila. These orthologs are primarily distributed across three operons: pilABCD, pilMNOPQ, and pilVWXY. While the first two operons are commonly found in all experimental genomes, the presence of the pilVWXY operon, coding for 11 orthologs, is reported here for the first time in A. hydrophila. Notably, the complete pilVWXY operon is absent in nonvirulent strains. A genomic islands study between a nonvirulent and hypervirulent strain also confirms absence of most of the genes coded by pilVWXY in nonvirulent strain. Interestingly, among the 51 experimental genomes analyzed, the pilVWXY operon was completely absent in 10 strains, most of which are categorized as nonvirulent; Conclusions: The distribution of two major type IV pili (T4P) nano-machines, PilABCDMNOPQ and PilVWXY, is reported here for the first time in A. hydrophila. Additionally, this study suggests a potential role for the PilVWXY nano-machine in establishing human disease.
Interrelation between Stress Management and Secretion Systems of Ralstonia solanacearum: An In Silico Assessment Goutam Banerjee, Fu-Shi Quan, Amit Mondal, Shantanu Sur, Pratik Banerjee, Pritam Chattopadhyay Pathogens, 2022 Ralstonia solanacearum (Rs), the causative agent of devastating wilt disease in several major and minor economic crops, is considered one of the most destructive bacterial plant pathogens. However, the mechanism(s) by which Rs counteracts host-associated environmental stress is still not clearly elucidated. To investigate possible stress management mechanisms, orthologs of stress-responsive genes in the Rs genome were searched using a reference set of known genes. The genome BLAST approach was used to find the distributions of these orthologs within different Rs strains. BLAST results were first confirmed from the KEGG Genome database and then reconfirmed at the protein level from the UniProt database. The distribution pattern of these stress-responsive factors was explored through multivariate analysis and STRING analysis. STRING analysis of stress-responsive genes in connection with different secretion systems of Rs was also performed. Initially, a total of 28 stress-responsive genes of Rs were confirmed in this study. STRING analysis revealed an additional 7 stress-responsive factors of Rs, leading to the discovery of a total of 35 stress-responsive genes. The segregation pattern of these 35 genes across 110 Rs genomes was found to be almost homogeneous. Increasing interactions of Rs stress factors were observed in six distinct clusters, suggesting six different types of stress responses: membrane stress response (MSR), osmotic stress response (OSR), oxidative stress response (OxSR), nitrosative stress response (NxSR), and DNA damage stress response (DdSR). Moreover, a strong network of these stress responses was observed with type 3 secretion system (T3SS), general secretory proteins (GSPs), and different types of pili (T4P, Tad, and Tat). To the best of our knowledge, this is the first report on overall stress response management by Rs and the potential connection with secretion systems.
Arms race between insecticide and insecticide resistance and evolution of insect management strategies Pritam Chattopadhyay, Goutam Banerjee Pesticides in Crop Production Physiological and Biochemical Action, 2020 The regular and constitutive application of insecticides creates a selective pressure for the emerging resistant pest, which is a big threat for the agricultural, horticultural, and public health sectors. In this chapter, we have summarized the mode of action of different insecticidal agents (chemical and biological), and resistant mechanism of insect pests against these agents. Basically, there is no difference in the process of resistance between chemical and biological insecticides. In general, four major events have been recognized, which are responsible for conferring resistance in insect pests: (i) alterations in insecticides, (ii) alteration of insecticide-receptor interactions, (iii) alterations in insecticide detoxification metabolism, and (iv) alteration in insecticide solubilization. Common techniques like “refuge strategy” only delay the resistance appearance time in pests, and thus is not a permanent solution. Therefore, complete solution/novel strategies should be introduced to overcome this situation. Here we have addressed several technical strategies which are currently applied in the horticulture, health programmes, and the agricultural field: (i) continuous search for new and novel insecticidal toxins from unexplored microbiome, (ii) exploring novel insect pest resistant varieties with the help of high throughput technologies like TILLING and Eco-TILLING, (iii) combining known insecticides in appropriate proportion with the help of genetic engineering, and (iv) modifying known insecticidal toxins with the help of protein engineering. The combination of these strategies along with existing insecticide resistance management will not only control the insect pest, but will also delay the process of resistance evolution.
Intrinsic role of bacterial secretion systems in phylogenetic niche conservation of Bradyrhizobium spp. Goutam Banerjee, Swarnendu Basak, Tathagato Roy, Pritam Chattopadhyay FEMS Microbiology Ecology, 2019 Bradyrhizobium is a biologically important bacterial genus. Different Bradyrhizobium strains exhibit distinct niche selection like free living, root nodular and stem nodular. The present in-silico study was undertaken to identify the role of bacterial secretome in the phylogenetic niche conservation (PNC) of Bradyrhizobium sp. Analysis was carried out with the publicly available 19 complete genome assembly and annotation reports. A protocol was developed to screen the secretome related genes using three different database, viz. genome, proteome and gene ortholog. This resulted into 139 orthologs that include type secretion systems (T1SS-T6SS) along with flagella (Flg), type IV pili (T4P) and tight adherence (Tad) systems. Multivariate analysis using bacterial secretome was undertaken to find out the role of these secretion systems in PNC. In free living strains, T3SS, T4SS and T6SS were completely absent. Whereas, in the stem nodulating strains, T3SS and T6SS were absent, but T4SS was found to be present. On the other hand, the T3SS was found to be present only in the root-nodulating strains. The present investigation clearly demonstrated a pattern of PNC based on the distribution of secretion system components. To the best of our knowledge, this is the first report on PNC of Bradyrhizobium using the multivariate analysis of secretome.
Structural and Functional Characterization of Type IV Pilus-Associated Proteins PilV, Pil94, and Pil96 of Aeromonas hydrophila : Potential Role in Human … A Bhattacharyya, G Banerjee, P Chattopadhyay Current Pharmaceutical Biotechnology , 2026 2026
Analysing the Metagenomic Dynamics of Soil Microbiota Affected by Tea Pruning and Skiffing Methods in Tea Plantations of Dibrugarh, Assam, India P Chattopadhyay, I Biswas, G Banerjee Indian Journal of Microbiology 65 (4), 2015-2020 , 2025 2025
Diversity and Distribution of Non-Reducing Polyketide Synthases (NR-PKSs) in Ascomycota (Fungi) P Chattopadhyay, G Banerjee Journal of Fungi 11 (9), 641 , 2025 2025 Citations: 1
Investigating the Distribution of Intimin and Invasin in Aeromonas hydrophila and Their Role in Host Tissue Attachment A Bhattacharyya, G Banerjee, P Chattopadhyay Bacteria 4 (1), 7 , 2025 2025
Probable Role of Type IV Pili of Aeromonas hydrophila in Human Pathogenicity A Bhattacharyya, G Banerjee, P Chattopadhyay Pathogens 13 (5), 365 , 2024 2024 Citations: 7
Role of SNRNAS in Biofilm Formation in Salmonella enterica Serovars Typhimurium and Enteritidis G Banerjee, P Chattopadhyay, P Banerjee IAFP 2022 , 2022 2022
Use of an abscisic acid-producing Bradyrhizobium japonicum isolate as biocontrol agent against bacterial wilt disease caused by Ralstonia solanacearum P Chattopadhyay, G Banerjee, PJ Handique Journal of Plant Diseases and Protection 129 (4), 869-879 , 2022 2022 Citations: 35
Interrelation between Stress Management and Secretion Systems of Ralstonia solanacearum: An In Silico Assessment G Banerjee, FS Quan, AK Mondal, S Sur, P Banerjee, P Chattopadhyay Pathogens 11 (7), 730 , 2022 2022 Citations: 5
Arms Race between Insecticide and Insecticide Resistance and Evolution of Insect Management Strategies P Chattopadhyay, G Banerjee Pesticides in Crop Production: Physiological and Biochemical Action, 109-130 , 2020 2020 Citations: 4
Intrinsic role of bacterial secretion systems in phylogenetic niche conservation of Bradyrhizobium spp. G Banerjee, S Basak, T Roy, P Chattopadhyay FEMS microbiology ecology 95 (11), fiz165 , 2019 2019 Citations: 5
Corticosteroid Catabolism by Klebsiella pneumoniae as a Possible Mechanism for Increased Pneumonia Risk P Chattopadhyay, G Banerjee Current pharmaceutical biotechnology 20 (4), 309-316 , 2019 2019 Citations: 4
Vanillin biotechnology: the perspectives and future G Banerjee, P Chattopadhyay Journal of the Science of Food and Agriculture 99 (2), 499-506 , 2019 2019 Citations: 381
Identification and Pathogenicity of Pseudomonas aeruginosa DJ1990 on Tail and Fin Rot Disease in Spotted Snakehead DJ Saikia, P Chattopadhyay, G Banerjee, B Talukdar, D Sarma Journal of the World Aquaculture Society 49 (4), 703-714 , 2018 2018 Citations: 11
Cleaner production of vanillin through biotransformation of ferulic acid esters from agroresidue by Streptomyces sannanensis P Chattopadhyay, G Banerjee, SK Sen Journal of Cleaner Production 182, 272-279 , 2018 2018 Citations: 96
Recent advancement on chemical arsenal of Bt toxin and its application in pest management system in agricultural field P Chattopadhyay, G Banerjee 3 Biotech 8 (4), 201 , 2018 2018 Citations: 52
Recent advancement on chemical arsenal of Bt toxin and its application in pest management system in agricultural field. 3 Biotech 8: 201 P Chattopadhyay, G Banerjee DOI: https://doi. org/10.1007/s13205-018-1223-1 , 2018 2018
Distinguishing Orchid Species by DNA Barcoding: Increasing the Resolution of Population Studies in Plant Biology P Chattopadhyay, G Banerjee, N Banerjee OMICS: A Journal of Integrative Biology 21 (12), 711-720 , 2017 2017 Citations: 38
Beneficial effects of bio-controlling agent Bacillus cereus IB311 on the agricultural crop production and its biomass optimization through response surface methodology G Banerjee, S Gorthi, P Chattopadhyay Anais da Academia Brasileira de Ciências 90, 2149-2159 , 2017 2017 Citations: 34
Recent trends of modern bacterial insecticides for pest control practice in integrated crop management system P Chattopadhyay, G Banerjee, S Mukherjee 3 Biotech 7 (1), 60 , 2017 2017 Citations: 141
Production, characterization and optimization of actinomycin D from Streptomyces hydrogenans IB310, a (n antagonistic bacterium against phytopathogens M Kulkarni, S Gorthi, G Banerjee, P Chattopadhyay Biocatalysis and Agricultural Biotechnology 10, 69-74 , 2017 2017 Citations: 40
MOST CITED SCHOLAR PUBLICATIONS
Vanillin biotechnology: the perspectives and future G Banerjee, P Chattopadhyay Journal of the Science of Food and Agriculture 99 (2), 499-506 , 2019 2019 Citations: 381
Biotechnological potential of natural food grade biocolorants P Chattopadhyay, S Chatterjee, SK Sen African Journal of Biotechnology 7 (17) , 2008 2008 Citations: 218
An overview of physico-chemical mechanisms of biogas production by microbial communities: a step towards sustainable waste management R Goswami, P Chattopadhyay, A Shome, SN Banerjee, AK Chakraborty, ... 3 Biotech 6 (1), 72 , 2016 2016 Citations: 181
Recent trends of modern bacterial insecticides for pest control practice in integrated crop management system P Chattopadhyay, G Banerjee, S Mukherjee 3 Biotech 7 (1), 60 , 2017 2017 Citations: 141
Microbial transformation of xenobiotics for environmental bioremediation S Sinha, P Chattopadhyay, I Pan, S Chatterjee, P Chanda, ... African Journal of Biotechnology 8 (22) , 2009 2009 Citations: 134
Cleaner production of vanillin through biotransformation of ferulic acid esters from agroresidue by Streptomyces sannanensis P Chattopadhyay, G Banerjee, SK Sen Journal of Cleaner Production 182, 272-279 , 2018 2018 Citations: 96
Recent advancement on chemical arsenal of Bt toxin and its application in pest management system in agricultural field P Chattopadhyay, G Banerjee 3 Biotech 8 (4), 201 , 2018 2018 Citations: 52
Bioremediation: a tool for cleaning polluted environments S Chatterjee, P Chattopadhyay, S Roy, SK Sen Journal of Applied Biosciences 11 (1), 594 , 2008 2008 Citations: 52
Characterization of Red Pigment from <i>Monascus</i> in Submerged Culture Red Pigment from <i>Monascus purpureus</i> S Chatterjee, S Maity, P Chattopadhyay, A Sarkar, S Laskar, SK Sen Journal of Applied Sciences Research 5 (12), 2102-2108 , 2009 2009 Citations: 51
Production, characterization and optimization of actinomycin D from Streptomyces hydrogenans IB310, a (n antagonistic bacterium against phytopathogens M Kulkarni, S Gorthi, G Banerjee, P Chattopadhyay Biocatalysis and Agricultural Biotechnology 10, 69-74 , 2017 2017 Citations: 40
Distinguishing Orchid Species by DNA Barcoding: Increasing the Resolution of Population Studies in Plant Biology P Chattopadhyay, G Banerjee, N Banerjee OMICS: A Journal of Integrative Biology 21 (12), 711-720 , 2017 2017 Citations: 38
Use of an abscisic acid-producing Bradyrhizobium japonicum isolate as biocontrol agent against bacterial wilt disease caused by Ralstonia solanacearum P Chattopadhyay, G Banerjee, PJ Handique Journal of Plant Diseases and Protection 129 (4), 869-879 , 2022 2022 Citations: 35
Beneficial effects of bio-controlling agent Bacillus cereus IB311 on the agricultural crop production and its biomass optimization through response surface methodology G Banerjee, S Gorthi, P Chattopadhyay Anais da Academia Brasileira de Ciências 90, 2149-2159 , 2017 2017 Citations: 34
Virulence plasmid of <i>Aeromonas hydrophila</i> induces macrophage apoptosis and helps in developing systemic infection in mice T Majumdar, P Chattopadhyay, DR Saha, S Sau, S Mazumder Microbial pathogenesis 46 (2), 98-107 , 2009 2009 Citations: 25
Modulations in seed micromorphology reveal signature of adaptive species-diversification in Dendrobium (Orchidaceae) B Chaudhary, P Chattopadhyay, N Banerjee Open Journal of Ecology 4 (2), 33-42 , 2014 2014 Citations: 23
Understanding the Phylomorphological Implications of Pollinia from <i>Dendrobium</i> (Orchidaceae) B Chaudhary, P Chattopadhyay, N Verma, N Banerjee American Journal of Plant Sciences 3 (6), 816-828 , 2012 2012 Citations: 19
Genetic Characterization of Selected Medicinal <i>Dendrobium</i> (Orchidaceae) Species Using Molecular Markers P Chattopadhyay, N Banerjee, B Chaudhary Research Journal of Biology 2 (4), 117-125 , 2012 2012 Citations: 19
Time and Dose Dependent Effect of Pseudomonas aeruginosa Infection on the Scales of Channa punctata (Bloch) Through Light and Electron Microscopy DJ Saikia, P Chattopadhyay, G Banerjee, D Sarma Turkish Journal of Fisheries and Aquatic Sciences 17 (5), 871-876 , 2017 2017 Citations: 15
Exploring Agricultural Potential of <i>Serratia entomophila</i> AB2: Dual Property of Biopesticide and Biofertilizer P Chattopadhyay, S Chatterjee, SK Sen British Biotechnology Journal 2 (1), 1-12 , 2012 2012 Citations: 14
Systemic infestation of <i>Serratia entomophila</i> AB2 through plant tissue inferred protection against insect pest and fungal pathogens P Chattopadhyay, SK Sen African Journal of Microbiology Research 7 (21), 2651-2655 , 2013 2013 Citations: 12
