Yuliya Krasylenko

@prf.upol.cz

Department of Biotechnology Faculty of Science
Palacký University Olomouc

MAIN RESEARCH INTERESTS

• root and shoot parasitic plants (Cuscuta, Orobanche, Phelipanche, Striga, Lathraea, etc.) with a special emphasis on mistletoes (Viscum, Loranthus, Arceuthobium)
• parasitic plants control and management
• cell mechanisms of host colonization by parasitic plants involving cytoskeleton
• strigolactones signaling involving microtubules
• advanced microscopy and related image analysis
• molecular mechanisms of plant abiotic stress responses (UV-B, heavy metals, low temperatures)
• reactive oxygen (ROS)/nitrogen species (RNS) and calcium signaling
• tubulin post-translational modifications

CURRENT POSITION

2022 – present – Assistant Professor, Department of Biotechnology (Prof. RNDr. Jozef Šamaj, DrSci.), Palacký University of Olomouc, Czech Republic

POSITIONS HELD

2017 – 2022 – Junior researcher, Postdoc at the Laboratory of Cell Biology, Centre of the Region Haná for Biotechnological and Agricultural Research (Olomouc, Czech Republic), Palacký University

EDUCATION

2012 – PhD, specialization “Cytology, Cell Biology, Histology” (03.00.01), Institute of Food Biotechnology and Genomics NAS of Ukraine, Kyiv, Ukraine
2007 – Master of Science in Biochemistry with highest honors, National University of “Kyiv-Mohyla Academy”, Ukraine
2005 – Bachelor of Science in Biology, National University of “Kyiv-Mohyla Academy”, Ukraine
2001 – Diploma of Specialist with Highest Honors in Nursery, First Nursing School, Kyiv, Ukraine

RESEARCH, TEACHING, or OTHER INTERESTS

Agricultural and Biological Sciences, Plant Science, Ecology, Cell Biology

Scopus Publications

1044

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Vertical botany: airborne remote sensing as an emerging tool for mistletoe research
Azim Missarov, Yevhen Sosnovsky, Karol Rydlo, Olga Brovkina, Wouter H. Maes, Kamil Král, Martin Krůček, and Yuliya Krasylenko
Canadian Science Publishing
Mistletoe detection and sampling remain challenging for arborists, dendrologists, forest ecologists, and other specialists because of the limited access to host tree canopy. In this review, smart solutions for mistletoe detection based on airborne platforms are discussed. Airborne remote sensing (ARS) has the developing potential to provide rapid, accurate, and cost-efficient detection and research of mistletoe on tree level and large areas within the complex terrain. Herein, such mistletoe ARS research methods as image spectroscopy, infrared thermography, light detection and ranging, and structure from motion are overviewed.

Seed germination of Cistanche armena (Orobanchaceae), a rare endangered holoparasitic species endemic to Armenia
Yuliya Krasylenko, Adelá Hýlová, Yevhen Sosnovsky, Markéta Ulbrichová, Lukáš Spíchal, and Renata Piwowarczyk
Cambridge University Press (CUP)
AbstractThe obligate root parasite Cistanche armena is a recently rediscovered, extremely rare endangered species endemic to Armenia, specifically parasitizing camelthorn (Alhagi maurorum, Fabaceae) and saltwort (Salsola dendroides, Chenopodiaceae). Its populations are reputedly declining due to habitat destruction and biotic impacts. Since the only known means of its reproduction is via the seeds, understanding the mechanisms of breaking C. armena seed dormancy and germination along with the related aspects of the species’ biology is highly important both from fundamental (functional ecology and evolution) and applied (conservation and management) perspectives. Here, we present the first in vitro seed germination protocol for C. armena involving fluridone, a systemic herbicide targeting the carotenoid biosynthetic pathway. In addition, the seed micromorphology of C. armena is described using both light microscopy and lignin autofluorescence visualized by confocal laser scanning microscopy. The actin cytoskeleton in radicle cells of germinated C. armena seedlings is described for the first time, being the proof of seed viability. Further elaboration and application of the proposed germination protocol with the cultivation of C. armena on susceptible hosts are altogether seen as a valuable tool for the conservation of this species.

Druid Drone—A portable unmanned aerial vehicle with a multifunctional manipulator for forest canopy and mistletoe research and management
Yuliya Krasylenko, Karol Rydlo, Natalia Atamas, Yevhen Sosnovsky, Oleksii Horielov, Ivo Maceček, Olga Šamajová, Miroslav Ovečka, Tomáš Takáč, and Jozef Šamaj
Wiley
Abstract The forest canopy, as a biodiversity hotspot with many wildlife habitats, remains a difficult site to access for researchers and forest managers. Here, we present a novel, small, unmanned aerial vehicle, called the Druid Drone (DD), equipped with a multifunctional manipulator designed for tree crown management and biodiversity research. Its use is demonstrated by studies and the control of the widespread, obligatory hemiparasitic European mistletoe (Viscum album L.). The DD provides integrated pest management by canopy surveying, spraying and sampling of leaves/branches for further studies. The pilot and operator, using first person view goggles, handle the lightweight backpack‐sized drone (DJI Mavic 2 Pro, Air 2, Phantom 3 or custom Rotorama quadcopter) equipped with a manipulator and five replaceable 3D‐printed functional modules. These include the peripheral high‐resolution Observer camera for close, non‐invasive inspection of tree crowns, the entomological Collector unit for arthropod trapping, the forceps arm Sampler for soft plant tissue collection, the terminal Secator saw for cutting harder tree tissues such as twigs and the Green Doctor precision sprayer, which disperses growth regulators or other compounds for targeted tree and mistletoe management. This method offers a potential solution for safe, precise and minimally invasive research on forest canopy biodiversity in different forest types across the globe, tree health care and mistletoe management as compared to the manual work of arborists or the use of canopy cranes, forest helicopters and firearms.

Consuming and consumed: Biotic interactions of African mistletoes across different trophic levels
Yuliya Krasylenko, Tonjock Rosemary Kinge, Yevhen Sosnovsky, Natalia Atamas, Katamssadan Haman Tofel, Oleksii Horielov, and Gerhard Rambold
Wiley
AbstractMistletoes, as perennial hemiparasitic angiosperms that parasitize woody plants, are an important component of the highly diverse, endemically rich and mosaic African flora, which is attributed to the Holarctic, Paleotropical, and Cape Floristic kingdoms. The richness of African mistletoes from the Loranthaceae and Viscaceae, along with many aspects of their biology and ecology, was covered in the comprehensive monograph of Polhill and Wiens (1998, Mistletoes of Africa, Royal Botanic Gardens). The present review is devoted to the taxonomic and functional diversity of symbionts associated with mistletoes in Africa and adjacent islands that contribute to the major biological functions of mistletoes, such as establishment and growth, nutrition and fitness, resistance to external stresses, as well as pollination and dispersal. These functions are favored by more or less distinct sets of associated bionts, including host plants, animal herbivores, frugivorous birds, nectar‐ and pollen‐feeding insects, and endophytic microorganisms. A separate section is devoted to mistletoe epiparasitism as a special case of host selection. All these organisms, which are components of the mistletoe‐associated community and multitrophic network, define the role of mistletoes as keystone species. Some aspects of the symbiont communities are compared here with patterns reported for mistletoes from other continents, particularly to identify potential relationships that remain to be explored for the African species. In addition, properties of endophytic mistletoe associates that contribute to the plant's communication with coexisting organisms are considered. We also highlight the important gaps of knowledge of the functioning of mistletoe‐associated communities in Africa and indicate some applied issues that need future attention.Abstract in French is available with online material.

Arabidopsis Iron Superoxide Dismutase FSD1 Protects Against Methyl Viologen-Induced Oxidative Stress in a Copper-Dependent Manner
Pavol Melicher, Petr Dvořák, Yuliya Krasylenko, Alexey Shapiguzov, Jaakko Kangasjärvi, Jozef Šamaj, and Tomáš Takáč
Frontiers Media SA
Iron superoxide dismutase 1 (FSD1) was recently characterized as a plastidial, cytoplasmic, and nuclear enzyme with osmoprotective and antioxidant functions. However, the current knowledge on its role in oxidative stress tolerance is ambiguous. Here, we characterized the role of FSD1 in response to methyl viologen (MV)-induced oxidative stress in Arabidopsis thaliana. In accordance with the known regulation of FSD1 expression, abundance, and activity, the findings demonstrated that the antioxidant function of FSD1 depends on the availability of Cu2+ in growth media. Arabidopsis fsd1 mutants showed lower capacity to decompose superoxide at low Cu2+ concentrations in the medium. Prolonged exposure to MV led to reduced ascorbate levels and higher protein carbonylation in fsd1 mutants and transgenic plants lacking a plastid FSD1 pool as compared to the wild type. MV induced a rapid increase in FSD1 activity, followed by a decrease after 4 h long exposure. Genetic disruption of FSD1 negatively affected the hydrogen peroxide-decomposing ascorbate peroxidase in fsd1 mutants. Chloroplastic localization of FSD1 is crucial to maintain redox homeostasis. Proteomic analysis showed that the sensitivity of fsd1 mutants to MV coincided with decreased abundances of ferredoxin and photosystem II light-harvesting complex proteins. These mutants have higher levels of chloroplastic proteases indicating an altered protein turnover in chloroplasts. Moreover, FSD1 disruption affects the abundance of proteins involved in the defense response. Collectively, the study provides evidence for the conditional antioxidative function of FSD1 and its possible role in signaling.

Mistletoe Eradicator - A Novel Tool for Simultaneous Mechanical and Chemical Control of Mistletoe
Khurshid Ahmad Bhat, Sabina Akhtar, Naseer Ahmad Dar, Mohammad Iqbal Bhat, Farooq Ahmad Bhat, Rashid Rizwan, Oleksii Horielov, and Yuliya Krasylenko
MyJove Corporation
Some species of mistletoes, an aerial plant hemiparasites, play a Janus-like role in ecosystems by being rich biodiversity hotspots due to their trophic and topic associations with various organisms and at the same time acting as biological pirates, exhausting the host trees by stealing water and nutrients via the haustorium. Many crop and timber trees are attacked by mistletoes, for example, dwarf mistletoes parasitize conifers, representatives of genus Tapinanthus target guava, and Phoradendron mistletoe target pecans. Recently, an increasing infestation of the European mistletoe (Viscum album subsp. album L.) in monoculture stands of common walnuts (Juglans regia L.) was recorded for the first time during 2018 in different regions of Kashmir Valley, India. The common chemical approach based on the spraying of mistletoe exophytes with the growth regulators, ethephon and glyphosate, was not efficient since V. album resprouted in the next vegetation season. Hand pruning of mistletoe was time-consuming, and a polyethylene covering resulted in the localized rotting of host tissues. The most efficient approach to eradicate mistletoe was the use of a novel tool called Mistletoe Eradicator, which allows to use mechanical and chemical means of mistletoe control simultaneously. It consists of a manipulating pole with the terminal pruning saw having a container filled with the growth regulator, from which the liquid slowly drops onto the saw blade through the nozzle. It allows working both from the ground and in the tree canopy. After the targeted application of 5%-10% ethephon or glyphosate to the base of the just pruned V. album stems (stubs), exophyte resprouting was significantly reduced 9 months after the pruning as compared to the only pruned or sprayed exophytes. An alternative variant of Mistletoe Eradicator was constructed in Ukraine filled just with a food color additive to track the liquid dropping and was successfully tested.

Juniperus excelsa s. str. in crimea - differentiation and history inferred from genetic and morphological markers
Małgorzata Mazur, Katarzyna A. Jadwiszczak, Agnieszka Bona, Yuliya Krasylenko, Oleg Kukushkin, and Katarzyna Marcysiak
Walter de Gruyter GmbH
Abstract Juniperus excelsa s. str. (Greek juniper) in Crimea is a relic species on the limits of its range, and represents the Mediterranean flora in the Sub-Mediterranean part of the peninsula. Its origin and history in this area remains unresolved. We aimed to analyze phylogeny and potential demographic expansion of the juniper in the Crimea as well as to study its morphological differentiation. We analyzed plant material from 59 trees inhabiting eight populations. Genetic variation assessments were based on the four non-coding chloroplast DNA (cpDNA) fragments and the nuclear internal transcribed spacer region ITS1-5,8S-ITS2 (ITS). To examine the morphological differentiation, eight measured/counted traits of cones, seeds, and shoots were chosen and eight ratios were calculated. Morphological parameters were compared using ANOVA, Student’s t test, discrimination analysis and Kruskal-Wallis and U Mann-Whitney tests. Two cpDNA fragments were polymorphic and, in total, 10 cpDNA haplotypes were found. Haplotype diversity (Hd) ranged from 0.0 to 0.9. Based on both cpDNA and ITS sequences variation, phylogenetic analyses revealed a close relationship of the Crimean junipers to the individuals from other parts of the species range. In general, our molecular results confirmed the low level of genetic differentiation of J. excelsa individuals inhabiting different parts of the species range, likely resulting from a common ancestry. Only slight morphological differences were found between populations with different geographic location or habitat. The analyzes showed the distinctness of the populations from the southern part of the coast. Some unique morphological and molecular features of southern coastal populations imply that they are remnants of Late Pleistocene abundant forests. We suggest that the recent fragmentation of the Juniperus populations in the Crimean Peninsula could have arisen during the Atlantic period of the Holocene.

Viscum meyeri (Viscaceae)-a new name for Viscum anceps, an old-established mistletoe species endemic to southern Africa
YEVHEN SOSNOVSKY, YULIYA KRASYLENKO, and VIKTOR NACHYCHKO
Magnolia Press
In this paper, we propose a new replacement name “Viscum meyeri” for a southern African endemic species currently known as V. anceps E.Mey. ex Sprague. The latter name is unavailable for use, being illegitimate as a later homonym of V. anceps Spreng.—the basionym for the presently accepted name Phoradendron anceps (Spreng.) M.Gómez. In addition, the second-step lectotypification of the new and replaced names is conducted. One collection from K is designated here as a lectotype of V. meyeri (≡ V. anceps E.Mey. ex Sprague), and eight more collections from FR, HAL, HBG, K, P, and S are considered as isolectotypes.

GR24, A Synthetic Strigolactone Analog, and Light Affect the Organization of Cortical Microtubules in Arabidopsis Hypocotyl Cells
Yuliya Krasylenko, George Komis, Sofiia Hlynska, Tereza Vavrdová, Miroslav Ovečka, Tomáš Pospíšil, and Jozef Šamaj
Frontiers Media SA
Strigolactones are plant hormones regulating cytoskeleton-mediated developmental events in roots, such as lateral root formation and elongation of root hairs and hypocotyls. The latter process was addressed herein by the exogenous application of a synthetic strigolactone, GR24, and an inhibitor of strigolactone biosynthesis, TIS108, on hypocotyls of wild-type Arabidopsis and a strigolactone signaling mutant max2-1 (more axillary growth 2-1). Owing to the interdependence between light and strigolactone signaling, the present work was extended to seedlings grown under a standard light/dark regime, or under continuous darkness. Given the essential role of the cortical microtubules in cell elongation, their organization and dynamics were characterized under the conditions of altered strigolactone signaling using fluorescence microscopy methods with different spatiotemporal capacities, such as confocal laser scanning microscopy (CLSM) and structured illumination microscopy (SIM). It was found that GR24-dependent inhibition of hypocotyl elongation correlated with changes in cortical microtubule organization and dynamics, observed in living wild-type and max2-1 seedlings stably expressing genetically encoded fluorescent molecular markers for microtubules. Quantitative assessment of microscopic datasets revealed that chemical and/or genetic manipulation of strigolactone signaling affected microtubule remodeling, especially under light conditions. The application of GR24 in dark conditions partially alleviated cytoskeletal rearrangement, suggesting a new mechanistic connection between cytoskeletal behavior and the light-dependence of strigolactone signaling.

Signaling Toward Reactive Oxygen Species-Scavenging Enzymes in Plants
Petr Dvořák, Yuliya Krasylenko, Adam Zeiner, Jozef Šamaj, and Tomáš Takáč
Frontiers Media SA
Reactive oxygen species (ROS) are signaling molecules essential for plant responses to abiotic and biotic stimuli as well as for multiple developmental processes. They are produced as byproducts of aerobic metabolism and are affected by adverse environmental conditions. The ROS content is controlled on the side of their production but also by scavenging machinery. Antioxidant enzymes represent a major ROS-scavenging force and are crucial for stress tolerance in plants. Enzymatic antioxidant defense occurs as a series of redox reactions for ROS elimination. Therefore, the deregulation of the antioxidant machinery may lead to the overaccumulation of ROS in plants, with negative consequences both in terms of plant development and resistance to environmental challenges. The transcriptional activation of antioxidant enzymes accompanies the long-term exposure of plants to unfavorable environmental conditions. Fast ROS production requires the immediate mobilization of the antioxidant defense system, which may occur via retrograde signaling, redox-based modifications, and the phosphorylation of ROS detoxifying enzymes. This review aimed to summarize the current knowledge on signaling processes regulating the enzymatic antioxidant capacity of plants.

Parasites on parasites: hyper-, epi-, and autoparasitism among flowering plants
Yuliya Krasylenko, Jakub Těšitel, Gregorio Ceccantini, Mariana Oliveira‐da‐Silva, Václav Dvořák, Daniel Steele, Yevhen Sosnovsky, Renata Piwowarczyk, David M. Watson, and Luiza Teixeira‐Costa
American Journal of Botany Wiley

Invivo light-sheet microscopy resolves localisation patterns of FSD1, a superoxide dismutase with function in root development and osmoprotection
Petr Dvořák, Yuliya Krasylenko, Miroslav Ovečka, Jasim Basheer, Veronika Zapletalová, Jozef Šamaj, and Tomáš Takáč
Wiley
AbstractSuperoxide dismutases (SODs) are enzymes detoxifying superoxide to hydrogen peroxide while temporal developmental expression and subcellular localisation are linked to their functions. Therefore, we aimed here to reveal in vivo developmental expression, subcellular, tissue‐ and organ‐specific localisation of iron superoxide dismutase 1 (FSD1) in Arabidopsis using light‐sheet and Airyscan confocal microscopy. FSD1‐GFP temporarily accumulated at the site of endosperm rupture during seed germination. In emerged roots, it showed the highest abundance in cells of the lateral root cap, columella, and endodermis/cortex initials. The largest subcellular pool of FSD1‐GFP was localised in the plastid stroma, while it was also located in the nuclei and cytosol. The majority of the nuclear FSD1‐GFP is immobile as revealed by fluorescence recovery after photobleaching. We found that fsd1 knockout mutants exhibit reduced lateral root number and this phenotype was reverted by genetic complementation. Mutant analysis also revealed a requirement for FSD1 in seed germination during salt stress. Salt stress tolerance was coupled with the accumulation of FSD1‐GFP in Hechtian strands and superoxide removal. It is likely that the plastidic pool is required for acquiring oxidative stress tolerance in Arabidopsis. This study suggests new developmental and osmoprotective functions of SODs in plants.

Fatty acid composition in seeds of holoparasitic Orobanchaceae from the Caucasus region: Relation to species, climatic conditions and nutritional value
Karolina Ruraż, Renata Piwowarczyk, Peter Gajdoš, Yuliya Krasylenko, and Milan Čertík
Elsevier BV

YODA-HSP90 Module Regulates Phosphorylation-Dependent Inactivation of SPEECHLESS to Control Stomatal Development under Acute Heat Stress in Arabidopsis
Despina Samakovli, Tereza Tichá, Tereza Vavrdová, Miroslav Ovečka, Ivan Luptovčiak, Veronika Zapletalová, Anna Kuchařová, Pavel Křenek, Yuliya Krasylenko, Theoni Margaritopoulou,et al.
Elsevier BV

Seed micromorphology of representatives of holoparasitic Orobanchaceae genera from the Caucasus region and its taxonomic significance
RENATA PIWOWARCZYK, KAROLINA RURAŻ, YULIYA KRASYLENKO, JUSTYNA KASIŃSKA, and ÓSCAR SÁNCHEZ PEDRAJA
Magnolia Press
Obligate parasitic plant species from the Orobanchaceae family belong to the most critical genera of world flora. Due to their strongly reduced vegetative organ architecture, there arise many difficulties and mistakes in species identification, and extra characteristics of systematic importance are required. Seed micromorphology is a highly informative taxonomic criterion that helps to resolve ambiguities in plant taxonomy and evolution, and has proved to be a valuable complementary tool for Orobanchaceae species identification. In this study, seeds of 43 holoparasitic species from the Cistanche, Diphelypaea, Orobanche and Phelipanche genera collected in the Caucasus were subjected to micromorphological analysis. The Caucasus is one of the most important centres of world biodiversity, with many endangered and endemic parasitic plants. Twenty two quantitative/qualitative micromorphological seed parameters were analysed using stereo ZOOM, confocal scanning laser (CLSM) and scanning electron (SEM) microscopy. Three types of seed were established based on periclinal wall ornamentation: 1) clearly pitted sculpturing in all Cistanche, Diphelypaea, and most Orobanche seeds; 2) fibrillar and veined sculpturing in Phelipanche seeds; and 3) smooth, granular or rugged (very rarely visibly pitted) outer periclinal wall in O. coerulescens and O. colorata seeds. However, CLSM, used in present studies for Orobanchaceae seeds for the first time, revealed more details of wall lignification and ornamentation invisible in SEM images. The best micromorphological characteristics for species identification are the type of ornamentation of the periclinal wall, the width of anticlinal wall, the character of perforation, perforation length and width, the fibrillar character, and fibrillar and vein width. A comparison of the main characteristics distinguishing the seeds of Orobanchaceae is also presented and discussed.

The european mistletoe (Viscum album l.): Distribution, host range, biotic interactions, and management worldwide with special emphasis on ukraine
Yuliya Krasylenko, Yevhen Sosnovsky, Natalia Atamas, Grigory Popov, Volodymyr Leonenko, Kateřina Janošíková, Nadiya Sytschak, Karol Rydlo, and Dmytro Sytnyk
Canadian Science Publishing
The hemiparasitic European mistletoe, Viscum album L. (Viscaceae), displays a rapid and remarkable expansion into natural and urban ecosystems in Ukraine. The monitoring and management of this quickly spreading species is becoming increasingly difficult as new plant species become hosts. Unlike other local mistletoe species, the European mistletoe has a broad distribution and thus requires a countrywide pest status assessment for control. This review outlines the major taxonomic and evolutionary issues pertinent to V. album with an emphasis on the characters used to distinguish its five currently recognized subspecies. The review also provides an updated distribution map and host range for the three V. album subspecies in Ukraine [V. album subsp. album, V. album subsp. abietis (Wiesb.) Janch., and V. album subsp. austriacum (Wiesb.) Vollm.], addressing the current knowledge of their biology and ecology. A significant portion of the paper is devoted to the diversity of organisms associated with V. album, including herbivores, endophytes, and parasites, drawing particular attention to major pollination and dispersal vectors, as well as potential biocontrol agents for this mistletoe species.

Plasticity of soybean stomatal responses to arsenic and cadmium at the whole plant level
Terézia Gálusová, Beáta Piršelová, Ľubomír Rybanský, Yuliya Krasylenko, Patrik Mészáros, Alžbeta Blehová, Monika Bardáčová, Jana Moravčíková, and Ildikó Matušíková
HARD Publishing Company
Heavy metals perturbate water balance in plants and hence impact stomatal aperture. After longer exposure, stomatal development also is affected, and stomatal density and size can change. Two soybean (Glycine max) varieties were experimentally selected for contrasting tolerance to arsenic and cadmium. For these cultivars, natural variability in leaf epidermal cell parameters was detected. Pot plants at first assimilating leaf stage were exposed for 10 days to 5 mg kg-1 soil As3+ and 50 mg kg-1 soil Cd2+ (respectively). Metals accumulated primarily in roots and exerted relatively low impact on biomass. Despite this, we observed diverse adjustments of stomata and pavement cells. In cv. Bólyi 44 the stomatal size decreased upon stress treatment, possibly to avoid further water loss. In contrast, the other cultivar Cordoba uses larger stomata that might be advantageous in gaining further resources. The observed responses varied depending on leaf type. In addition, dorsoventral stomatal responses in width, yet undescribed under metal stress, were observed. Our data show that leaf epidermal cell adjustments are flexible components of plant defense even at low metal doses, and possibly help to compromise the structural and functional needs of plant (tissue) under metal stress.

Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures
Svitlana H. Plohovska, Yuliya A. Krasylenko, and Alla I. Yemets
Wiley
AbstractCytoskeleton is gaining the increasing recognition as one of nitric oxide (NO)—downstream targets because of its involvement in plenty of NO‐controlled processes in plants throughout the entire life cycle starting from seed germination to pollination as well as (a)biotic stress tolerance. It has been revealed that low temperature (+0.5°C) has an inhibitory effect on A. thaliana primary root growth and causes an anisotropic increase of epidermal cells diameter in elongation zone. Furthermore, actin filaments’ organization of epidermal cells in different zones of primary roots is modulated by NO content. Thus, the exogenous NO donor (SNP) favors to actin filaments network reorganization, while both cold and NO scavenger (c‐PTIO) increase its randomization. According to the data obtained, it can be assumed that not only actin filaments act as NO sensors, but NO is also involved into plant cell response on low temperatures by the signaling via such important cytoskeleton machinery as actin network.

Nitric oxide synthase inhibitor L-NAME affects Arabidopsis root growth, morphology, and microtubule organization
Yuliya A. Krasylenko, Alla I. Yemets, and Yaroslav B. Blume
Wiley
AbstractThe presence of evolutionarily conserved NOS or NOS‐like enzymes in land plants different than those in animals is still unclear, despite their activity has been revealed in cytosol and some organelles. At the same time, the emerging evidence for the importance of L‐arginine‐dependent pathways of NO synthesis in plant cells is still accumulating. The aim of our study was to reveal physiological effects on growth and differentiation processes, and microtubular cytoskeleton organization of the competitive mammalian NO synthase inhibitor Nω‐nitro‐L‐arginine methylester (L‐NAME). Thus, the treatment of Arabidopsis with L‐NAME (50–1 mM) caused dose‐ and time‐dependent inhibition of primary roots growth. Moreover, the morphology of primary roots under the influence of L‐NAME also underwent changes. L‐NAME (>100 µM) induced the formation of novel over‐elongated root hairs in shortened elongation zone, while in higher concentrations (500 µM) it caused a slight swelling of epidermal cells in differentiation zone. L‐NAME also provoked microtubule reorganization in epidermal cells of different root growth zones. Thus, L‐NAME at concentrations of 50–1 mM induced cortical microtubules randomization and/or depolymerization in epidermal cells of the root apex, meristem, transition, elongation, and differentiation zones after 2 h of treatment. Disordered microtubules in trichoblasts could initiate the formation of actively elongating root hairs that reveals longitudinal microtubules ensuring their active growth at 24 h of treatment. Therefore, L‐NAME inhibits primary root growth, induces the differentiation processes in roots, reorganizes cortical microtubules in epidermal root cells suggesting the importance of L‐arginine‐dependent pathways of NO synthesis in plants.

First report of eastern dodder (Cuscuta monogyna) parasitizing common fig (Ficus carica) at the Crimean Peninsula
Y. A. Krasylenko and R. Piwowarczyk
Scientific Societies

Species-specific differences in architecture and chemical composition of dodder seeds
Michaela Martinčová, Peter Kaštier, Yuliya A. Krasylenko, Peter Gajdoš, Milan Čertík, Ildikó Matušíková, and Alžbeta Blehová
Elsevier BV

Cytoskeleton in the parasitic plant cuscuta during germination and prehaustorium formation
Peter Kaštier, Yuliya A. Krasylenko, Michaela Martinčová, Emmanuel Panteris, Jozef Šamaj, and Alžbeta Blehová
Frontiers Media SA
Although cytoskeleton is a driving force for cell division and growth in higher plants, there is little evidence about its components in parasitic angiosperms. Microtubules and actin filaments in cells of shoot apical meristem and root-like structure of stem holoparasites European (C. europaea L.) and Eastern (C. monogyna Vahl.) dodders, as well as in prehaustorium, the specific organ adapted to parasitism, were visualized for the first time by immunolabeling and fluorescence microscopy. The significance of cytoskeletal elements during germination and prehaustorium formation was addressed by treatments with taxol, oryzalin, latrunculin B, cytochalasin B/D, jasplakinolide, and 2,3-butanedione monoxime. In shoot apical meristem many dividing cells were observed, in contrast to root-like structure, devoid of cell divisions. Cortical microtubules were oriented transversely and/or obliquely, while actin filaments were randomly distributed in cells of both organs. Furthermore, longitudinal cortical microtubules were present in digitate cells of prehaustorium, and transverse arrays were found in its file cells. Long and short random actin filaments were also observed in prehaustorium cells. Thus, it was shown that the cytoskeleton in dodder shoot cells is organized in a similar way to non-parasitic dicots, while cytoskeletal organization has some peculiarities in quickly senescing root-like structure and prehaustorium.

Juniper dwarf mistletoe (Arceuthobium oxycedri) in the Crimean Peninsula: Novel insights into its morphology, hosts, and distribution
Yuliya A. Krasylenko, Kateřina Janošíková, and Oleg V. Kukushkin
Canadian Science Publishing
Juniper dwarf mistletoe (Arceuthobium oxycedri (DC.) M. Bieb) is a semiparasitic species of Holarctic Cupressaceae hosts. This study updates the status of A. oxycedri and its hosts at the Crimean Peninsula, clearly defining them as principal (Eastern prickly juniper, Juniperus deltoides R.P. Adams), secondary (Greek juniper, J. excelsa M. Bieb.), occasional (rare) (Oriental thuja, Platycladus orientalis (L.) Franco), and uncommon (other representatives of Juniperus, Cupressus, and Chamaecyparis genera). Using eight morphological characteristics (aerial shoot length, basal diameter, 1st/2nd/3rd internode lengths and widths), we investigated aerial shoot morphology of male and female A. oxycedri plants on three hosts and found host-dependent differences. Parasites on P. orientalis, a nonprincipal host, at different sites are smaller by comparison with both juniper hosts. Anthesis, fruit ripening, and seed release are host-dependent as well. Two fly species (Diptera), common greenbottle (Lucilia ceasar L.), ...

INVOLVEMENT OF PLANT CYTOSKELETON INTO CELLULAR MECHANISMS OF METALS TOXICITY

Involvement of plant cytoskeleton in cellular mechanisms of metal toxicity
I. I. Horiunova, Yu. A. Krasylenko, A. I. Yemets, and Ya. B. Blume
Allerton Press

RECENT SCHOLAR PUBLICATIONS

Persephone’s Flower: Ecology and Development of Lathraea squamaria (Orobanchaceae), an Unusual Root Holoparasitic Plant
Y Krasylenko, L Teixeira-Costa, YY Sosnovsky, O Šamajov, MM Ovečka, ...
2024

Vertical botany: airborne remote sensing as an emerging tool for mistletoe research
A Missarov, Y Sosnovsky, K Rydlo, O Brovkina, WH Maes, K Krl, ...
Botany 102 (3), 58-71 2023

Seed germination of Cistanche armena (Orobanchaceae), a rare endangered holoparasitic species endemic to Armenia
Y Krasylenko, A Hlov, Y Sosnovsky, M Ulbrichov, L Spchal, ...
Seed Science Research 33 (2), 118-125 2023

Druid Drone—A portable unmanned aerial vehicle with a multifunctional manipulator for forest canopy and mistletoe research and management
Y Krasylenko, K Rydlo, N Atamas, Y Sosnovsky, O Horielov, I Maceček, ...
Methods in Ecology and Evolution 14 (6), 1416-1423 2023

Consuming and consumed: Biotic interactions of African mistletoes across different trophic levels
Y Krasylenko, TR Kinge, Y Sosnovsky, N Atamas, KH Tofel, O Horielov, ...
Biotropica 54 (4), 1103-1119 2022

Arabidopsis Iron Superoxide Dismutase FSD1 Protects Against Methyl Viologen-Induced Oxidative Stress in a Copper-Dependent Manner
P Melicher, P Dvořk, Y Krasylenko, A Shapiguzov, J Kangasjrvi, ...
Frontiers in Plant Science 13, 823561 2022

Mistletoe Eradicator-A Novel Tool for Simultaneous Mechanical and Chemical Control of Mistletoe
KA Bhat, S Akhtar, NA Dar, MI Bhat, FA Bhat, R Rizwan, O Horielov, ...
J. Vis. Exp 181, e63455 2022

Viscum meyeri (Viscaceae)—A new name for Viscum anceps, an old-established mistletoe species endemic to southern Africa
Y Sosnovsky, Y Krasylenko, V Nachychko
Phytotaxa 523 (4), 284-290 2021

Juniperus excelsa s. str. in crimea–differentiation and history inferred from genetic and morphological markers
M Mazur, KA Jadwiszczak, A Bona, Y Krasylenko, O Kukushkin, ...
Folia Forestalia Polonica, Series A–Forestry 2021

GR24, a synthetic strigolactone analog, and light affect the organization of cortical microtubules in Arabidopsis hypocotyl cells
Y Krasylenko, G Komis, S Hlynska, T Vavrdov, M Ovečka, T Pospšil, ...
Frontiers in Plant Science 12, 675981 2021

Signaling toward reactive oxygen species-scavenging enzymes in plants
P Dvořk, Y Krasylenko, A Zeiner, J Šamaj, T Takč
Frontiers in Plant Science 11, 618835 2021

Parasites on parasites: hyper‐, epi‐, and autoparasitism among flowering plants
Y Krasylenko, J Těšitel, G Ceccantini, M Oliveira‐da‐Silva, V Dvořk, ...
American Journal of Botany 108 (1), 8-21 2021

In vivo light‐sheet microscopy resolves localisation patterns of FSD1, a superoxide dismutase with function in root development and osmoprotection
P Dvořk, Y Krasylenko, M Ovečka, J Basheer, V Zapletalov, J Šamaj, ...
Plant, Cell & Environment 44 (1), 68-87 2021

Cortical microtubule remodelling during strigolactone-and light-mediated growth inhibition of Arabidopsis hypocotyls
YA Krasylenko, G Komis, S Hlynska, T Vavrdov, M Ovečka, T Pospšil, ...
bioRxiv, 2020.12. 07.414524 2020

Fatty acid composition in seeds of holoparasitic Orobanchaceae from the Caucasus region: Relation to species, climatic conditions and nutritional value
K Ruraż, R Piwowarczyk, P Gajdoš, Y Krasylenko, M Čertk
Phytochemistry 179, 112510 2020

Plasticity of soybean stomatal responses to arsenic and cadmium at the whole plant level
T Glusov, B Piršelov, Ľ Rybansk, Y Krasylenko, P Mszros, ...
Pol. J. Environ. Stud 29, 3569-3580 2020

FSD1: developmentally‐regulated plastidial, nuclear and cytoplasmic enzyme with anti‐oxidative and osmoprotective role
P Dvořk, Y Krasylenko, M Ovečka, J Basheer, V Zapletalov, J Šamaj, ...
Plant, cell & environment 2020

YODA-HSP90 module regulates phosphorylation-dependent inactivation of SPEECHLESS to control stomatal development under acute heat stress in Arabidopsis
D Samakovli, T Tich, T Vavrdov, M Ovečka, I Luptovčiak, V Zapletalov, ...
Molecular plant 13 (4), 612-633 2020

FSD1: a plastidial, nuclear and cytoplasmic enzyme relocalizing to the plasma membrane under salinity
P Dvořk, Y Krasylenko, M Ovečka, J Basheer, V Zapletalov, J Šamaj, ...
bioRxiv, 2020.03. 24.005363 2020

The European mistletoe (Viscum album L.): distribution, host range, biotic interactions, and management worldwide with special emphasis on Ukraine
Y Krasylenko, Y Sosnovsky, N Atamas, G Popov, V Leonenko, ...
Botany 98 (9), 499-516 2020

MOST CITED SCHOLAR PUBLICATIONS

Signaling toward reactive oxygen species-scavenging enzymes in plants
P Dvořk, Y Krasylenko, A Zeiner, J Šamaj, T Takč
Frontiers in Plant Science 11, 618835 2021
Citations: 265

Nitric oxide signalling via cytoskeleton in plants
AI Yemets, YA Krasylenko, DI Lytvyn, YA Sheremet, YB Blume
Plant Science 181 (5), 545-554 2011
Citations: 80

Nitric oxide as a critical factor for perception of UV‐B irradiation by microtubules in Arabidopsis
YA Krasylenko, AI Yemets, YA Sheremet, YB Blume
Physiologia plantarum 145 (4), 505-515 2012
Citations: 61

Tubulin tyrosine nitration regulates microtubule organization in plant cells
YB Blume, YA Krasylenko, OM Demchuk, AI Yemets
Frontiers in Plant Science 4, 530 2013
Citations: 59

Plant‐based biopharming of recombinant human lactoferrin
AI Yemets, IV Tanasienko, YA Krasylenko, YB Blume
Cell Biology International 38 (9), 989-1002 2014
Citations: 49

Plant microtubules reorganization under the indirect UV-B exposure and during UV-B-induced programmed cell death
YA Krasylenko, AI Yemets, YB Blume
Plant Signaling & Behavior 8 (5), e24031 2013
Citations: 35

Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures
SH Plohovska, YA Krasylenko, AI Yemets
Cell Biology International 43 (9), 1020-1030 2019
Citations: 31

The role of the plant cytoskeleton in phytohormone signaling under abiotic and biotic stresses
YB Blume, YA Krasylenko, AI Yemets
Mechanism of plant hormone signaling under stress 2, 127-185 2017
Citations: 23

Involvement of plant cytoskeleton in cellular mechanisms of metal toxicity
II Horiunova, YA Krasylenko, AI Yemets, YB Blume
Cytology and Genetics 50, 47-59 2016
Citations: 22

Plasticity of soybean stomatal responses to arsenic and cadmium at the whole plant level
T Glusov, B Piršelov, Ľ Rybansk, Y Krasylenko, P Mszros, ...
Pol. J. Environ. Stud 29, 3569-3580 2020
Citations: 16

Loranthus europaeus (Loranthaceae) in Ukraine: an overview of distribution patterns and hosts
RY Gleb, OD Volutsa
Ukrainian Botanical Journal 76 (5), 406-417 2019
Citations: 16

Cell mechanisms of nitric oxide signaling in plants under abiotic stress conditions
YA Krasylenko, AI Yemets, YB Blume
Mechanism of Plant Hormone Signaling under Stress 1, 371-398 2017
Citations: 15

Nitric oxide and UV-B radiation
AI Yemets, YA Krasylenko, YB Blume
Nitric Oxide Action in Abiotic Stress Responses in Plants, 141-154 2015
Citations: 15