GIUSEPPE ROFRANO
@izsmportici.it/portale
National Reference Centre for the analysis and study of correlations between the environment, animals and humans
ISTITUTO ZOOPROFILATTICO SPERIMENTALE DEL MEZZOGIORNO
RESEARCH INTERESTS
Giuseppe Rofrano currently works at the Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno. Giuseppe did research on Genetics, Molecular Biology and Agricultural Plant Science; crops of interest include pepper and artichoke. Now he's working at the National Reference Center for the analysis and study of correlation between environment, animal and human.
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Daniel Signorelli, Luigi Jacopo D’Auria, Antonio Di Stasio, Alfonso Gallo, Augusto Siciliano, Mauro Esposito, Alessandra De Felice, and Giuseppe Rofrano
MDPI AG
Honeybees and hive products have long been recognized as excellent bio-indicators, as they provide valuable information on the state of the environments concerned. As yet, however, no tool is capable of contextually assessing the level of pollution of different environmental matrices in order to create maps of areas in which to place hives. In this paper, a possible method of creating a regional map to support the implementation of targeted monitoring plans on beekeeping is described. We obtained and identified related-activity pressure factors, which were subsequently combined by means of a multi-criteria approach through the analytic hierarchy process method (AHP). The different levels used were drawn up by first attributing qualitative values; these were converted into quantitative values through scoring elaborations and pair comparisons and then elaborated and standardized by means of different techniques in order to create an index with a spatial distribution of five risk classes throughout the region. To verify the correct execution of the procedure, a consistency ratio method was implemented on this index and validated the reliability of the application as the main source of information for sampling activities on beekeeping products. Creation of the specific environmental risk index enabled us to construct a map displaying the areas of greatest impact on beekeeping activities and a representation of the cumulative effects generated by the different pollutants in the air, water, soil and subsoil compartments. This index may, therefore, constitute an essential tool to support beekeepers in choosing sites for their apiaries.
Paolo Montuori, Elvira De Rosa, Pellegrino Cerino, Antonio Pizzolante, Federico Nicodemo, Alfonso Gallo, Giuseppe Rofrano, Sabato De Vita, Antonio Limone, and Maria Triassi
MDPI AG
The aim of this study was to evaluate the concentrations of polycyclic aromatic hydrocarbons (PAHs) in 1168 groundwater samples of the Campania Plain (Southern Italy), taken using a municipal environmental pressure index (MIEP), and to analyze the distribution of these compounds to determine source PAHs using ratios of isomers diagnostic. Lastly, this study also aimed to estimate the potential health cancer risk in groundwaters. The data indicated that the highest concentration of PAHs was found in groundwater from Caserta Province and the contents of BghiP, Phe, and Nap were detected in the samples. The spatial distribution of these pollutants was evaluated using the Jenks method; moreover, the data indicated that incremental lifetime cancer risk ILCRingestion ranged from 7.31 × 10−20 to 4.96 × 10−19, while ILCRdermal ranged from 4.32 × 10−11 to 2.93 × 10−10. These research findings may provide information about the Campania Plain’s groundwater quality and aid in the development of preventative measures to lessen PAH contamination in groundwater.
Annalise Guarino, Stefano Albanese, Domenico Cicchella, Pooria Ebrahimi, Salvatore Dominech, Lucia Rita Pacifico, Giuseppe Rofrano, Federico Nicodemo, Antonio Pizzolante, Carolina Allocca,et al.
Elsevier BV
Davide Cardinale, Maria Tafuro, Andrea Mancusi, Santa Girardi, Federico Capuano, Yolande Thérèse Rose Proroga, Federica Corrado, Jacopo Luigi D’Auria, Annachiara Coppola, Giuseppe Rofrano,et al.
MDPI AG
The SARS-CoV-2 can spread directly via saliva, respiratory aerosols and droplets, and indirectly by contact through contaminated objects and/or surfaces and by air. In the context of COVID-19 fomites can be an important vehicle of virus transmission and contribute to infection risk in public environments. The aim of the study was to analyze through surface sampling (sponge method) the presence of SARS-CoV-2 in public and working environments, in order to evaluate the risk for virus transmission. Seventy-seven environmental samples were taken using sterile sponges in 17 animal farms, 4 public transport buses, 1 supermarket and 1 hotel receptive structure. Furthermore, 246 and 93 swab samples were taken in the farms from animals and from workers, respectively. SARS-CoV-2 detection was conducted by real-time RT-PCR and by digital droplet RT-PCR (dd RT-PCR) using RdRp, gene E and gene N as targets. None of the human and animal swab samples were positive for SARS-CoV-2, while detection was achieved in 20 of the 77 sponge samples (26%) using dd RT-PCR. Traces of the RdRp gene, gene E and gene N were found in 17/77 samples (22%, average concentration 31.2 g.c./cm2, range 5.6 to 132 g.c./cm2), 8/77 samples (10%, average concentration 15.1 g.c./cm2, range 6 to 36 g.c./cm2), and in 1/77 (1%, concentration 7.2 g.c./cm2). Higher detection rates were associated with sampling in animal farms and on public transport buses (32% and 30%) compared to the supermarket (21%) and the hotel (no detection). The result of the study suggests that the risk of contamination of surfaces with SARS-CoV-2 increases in environments in which sanitation strategies are not suitable and/or in highly frequented locations, such as public transportation. Considering the analytical methods, the dd RT-PCR was the only approach achieving detection of SARS-CoV-2 traces in environmental samples. Thus, dd RT-PCR emerges as a reliable tool for sensitive SARS-CoV-2 detection.
Biancamaria Pierri, Maria Tafuro, Maria Concetta Cuomo, Denise Di Concilio, Lucia Vassallo, Andrea Pierri, Amedeo Ferro, Giuseppe Rofrano, Alfonso Gallo, Antonio Di Stasio,et al.
Frontiers Media SA
The aim of the present study is to assess saliva as a reliable specimen for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection by real-time reverse transcription-PCR (RT-PCR), especially in community mass screening programs. The performance analysis considered 1,221 total samples [nasopharyngeal (NP) swabs and corresponding saliva], tested by means of a reference diagnostic real-time RT-PCR assay. Conflicting results were further investigated with a second, more sensitive, reference assay. Analysis of agreement showed a good concordance (95.82%), with a k coefficient value of.74 (p < 0.001); moreover, a follow-up analysis revealed the presence of viral gene targets in saliva samples at the time point the corresponding NP swabs turned negative. Data obtained prove the reliability of this alternative biofluid for SARS-CoV-2 detection in real-time RT-PCR. Considering the role of saliva in the coronavirus disease 2019 (COVID-19) transmission and pathogenesis, and the advantages in the use of salivary diagnostics, the present validation supports the use of saliva as an optimal choice in large-scale population screening and monitoring of the SARS-CoV-2 virus.
Annachiara Coppola, Carlo Buonerba, Davide Cardinale, Gabriella Lo Conte, Donato Sansone, Giuseppe Rofrano, Sabato De Vita, Maria Morgante, Maria Triassi, Luigi Atripaldi,et al.
Frontiers Media SA
As of November 17, 2021, SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus 2), the causative agent of COVID-19 (COronaVIrus Disease 19), has infected ~250 million people worldwide, causing around five million deaths. Titers of anti-SARS-CoV-2 neutralizing antibodies were relatively stable for at least 9 months in a population-based study conducted in Wuhan, China, both in symptomatic and in asymptomatic individuals. In the mass screening campaign conducted in the town of Ariano Irpino (Avellino, Italy) in May, 2020, 5.7% (95% CI: 5.3-6-1) of the 13,444 asymptomatic citizens screened were positive for anti-nucleocapsid antibodies against SARS-CoV-2. Among these, 422 citizens were re-tested for anti SARS-CoV-2 antibodies in January, 2021 and/or in April, 2021 and enrolled in this longitudinal observational study. Median (interquartile range) age of the study cohort was 46 years (29–59), with 47 (11.1%) participants of minor age, while 217 (51.4%) participants were females. There was no evidence of re-infection in any of the subjects included. Presence of anti-nuclear antibodies antibodies (Elecysis, Roche) was reported in 95.7 and 93.7% of evaluable participants in January and April, 2021. Multiple logistic regression analysis used to explore associations between age, sex and seroprevalence showed that adults vs. minors had significantly lower odds of having anti-S1 antibodies (Biorad) both in January, 2021 and in April, 2021. Our findings showed that antibodies remained detectable at least 11.5 months after infection in >90% of never symptomatic cases. Further investigation is required to establish duration of immunity against SARS-CoV-2.
Pellegrino Cerino, Carlo Buonerba, Gianfranco Brambilla, Luigi Atripaldi, Maria Tafuro, Denise Di Concilio, Lucia Vassallo, Gabriella Lo Conte, Maria Concetta Cuomo, Ivana Maiello,et al.
Future Science Ltd
SARS-CoV-2, the causative agent of the COVID-19 pandemic, has rarely been associated with transmission from humans to animals (reverse zoonotic transmission). In this retrospective study, the authors reviewed data obtained from 236 animals, including buffaloes, goats/sheep, horses, carrier pigeons, rabbits, hens, snakes, pigs and cows that were screened for SARS-CoV-2 infection because they had been in contact with their SARS-CoV-2-positive breeder for at least 2 weeks. None of the tested animals were found to be positive. The authors' findings suggest that the risk of reverse zoonotic transmission among bred animals and SARS-CoV-2-positive breeders is very low or nonexistent. Additional studies are warranted.
G. Rofrano, N. Trotta, R. Pepe, M.A. Pagnotta, F. Saccardo, and T. Cardi
International Society for Horticultural Science (ISHS)
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)
1. ROFRANO G., TROTTA N., PAGNOTTA M.A., SACCARDO F., VIVONE A., MENNELLA G., FRANCESE G., CARDI T. e PEPE R., 2014. - Omogeneità e stabilità morfo-genetica di varietà locali Campane di carciofo. X Convegno Nazionale Sulla Biodiversità, Roma, 3-5 Settembre 2014. Atti convegno,
2. PEPE R., BACCO A, ROFRANO G., TROTTA N., 2014. Network per la salvaguardia e la gestione delle risorse genetiche agro-alimentari campane (Acronimo:Agrigenet). X Convegno Nazionale Sulla Biodiversità, Roma, 3-5 Settembre 2014. Atti convegno,
3. TROTTA N., ROFRANO G., BACCO A., CARDI T., PEPE R., 2014. Procedure per la valutazione di semi di accessioni locali di phaseolus vulgaris L. X Convegno Nazionale Sulla Biodiversità, Roma, 3-5 Settembre 2014. Atti convegno,
4. TROTTA N., BACCO A., LO TAURO A., ROFRANO G., ONOFARO SANAJÀ V., VIVONE A., CARDI T., PEPE R., 2013. Valutazione della stabilità morfologica in alcuni genotipi di pomodoro candidabili all’iscrizione al Registro Nazionale delle Varietà Vegetali. Notiziario RGV (1-2): 60-65. ISSN: 1974-2738.
5. ACCIARRI N.; SABATINI E.; CAMPION B.; PARISI M.; PENTANGELO A.; PEPE R.; TROTTA N.; ROFRANO G., 2013. Pomodoro. In: Conservazione biodiversità, gestione banche dati e miglioramento genetico- BIODATI VOL I. 392-396. ISBN: 9788868120986. DOI: 10.4458/0986
6. NERVO G., FALAVIGNA A., SCOSSA F., PARISI M., D’AGOSTINO N., PEPE R., TROTTA R., E ROFRANO G., 2013. Peperone. In: Conservazione biodiversità, gestione banche dati e miglioramento genetico- BIODATI VOL I. . ISBN: 9788868120986. DOI: 10.4458/0986
7. CAMPION B.; ZACCARDELLI M.; PERRONE D.; PEPE R.; TROTTA N.; ROFRANO G., 2013. Fagiolo e altre leguminose da granella. In: Conservazione biodiversità, gestione banche dati e miglioramento genetico- BIODATI VOL I. . ISBN: 9788868120986. DOI: 10.4458/0986
8. FERRARI V., PICCININI E., CAIONI M., PEPE R., TROTTA N., ROFRANO G., 2013. Carciofo. In: Conservazione biodiversità, gestione banche dati e miglioramento genetico- BIODATI VOL I. . ISBN: 9788868120986. DOI: 10.4458/0986
9. PEPE R., TROTTA N., ROFRANO G., 2013. Zucche ed altre cucurbitaceae. In: Conservazione biodiversità, gestione banche dati e miglioramento genetico- BIODATI VOL I. . ISBN: 9788868120986. DOI: 10.4458/0986
10. PEPE R, TROTTA N., ROFRANO G., VIVONE A., TEDESCO P., DE VIVO G., MENNELLA G., D’ALESSANDRO A., FRANCESE G., CARDI T., 2013. – Schede valutative di alcuni genotipi di carciofo realizzate nell’ambito del progetto MiPAAF “CAR-VARVI-Valorizzazione di germoplasma di carciofo attraverso la costituzione varietale e il risanamento da virus, pp:100; ISBN: 978-88-87173-13-0.
11. ROFRANO G., TROTTA N.,PEPE R.,PAGNOTTA M.A.,SACCARDO F. AND CARDI T., 2013.
Improving the artichoke quality of Campania Region. Acta Horticulturae (ISHS) 983:31-37
12.ROFRANO G., FRANCESE G., D’ALESSANDRO A., PEPE R., TROTTA N., CARDI T. e MENNELLA G., 2013. Influenza della colonizzazione con micorrize sulla produttività e sul contenuto fenolico di alcuni genotipi campani di carciofi. Acta Italus hortus. 8:86-92. ISBN: 978-88-905628-5-3.
13. TROTTA N., ROFRANO G., FESTA G., VIVONE A., TEDESCO P., DE VIVO G., PEPE R., 2012. Caratterizzazione morfo-fisiologica di accessioni di peperone e pomodoro: omogeneità e distinguibilità di varietà da conservazione. RGV Notiziario Risorse Genetiche Vegetali MIPAAF Anno XII n.2-3 pp. 15-18. ISSN: 1974-2738.
14. PEPE R., FESTA G., DE VIVO G., TEDESCO P., ROFRANO G., TROTTA N., MENNELLA G., D’ALESSANDRO A., FRANCESE G. e CARDI T. 2011. Valorizzazione della biodiversità orticola in Campania. Notiziario Risorse Genetiche Vegetali, Anno XI, pagg. 11-13.
15. ROFRANO G., TROTTA N., PEPE R., PAGNOTTA M.A., SACCARDO F., 2011. - Obtaining F1 hybrids from the genetic material of artichoke landraces of Campania, restored and selected. Minerva Biotec 2011;23 Suppl. 1 to No. 2:7-8
16.ROFRANO G., ROSSI E., COSIMI S., 2009. Valutazione della resistenza agli insetticidi del Tribolium spp., Tecnica Molitoria,(8): 877-886. ISSN: 0040-1862