Neeraj Dwivedi

@manavrachna.edu.in

Associate Professor at Department of Biotechnology
Associate Professor

Neeraj Dwivedi


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https://researchid.co/neerajdwivedi.set

RESEARCH, TEACHING, or OTHER INTERESTS

Agricultural and Biological Sciences, Plant Science, Biochemistry, Genetics and Molecular Biology, Molecular Biology
14

Scopus Publications

237

Scholar Citations

7

Scholar h-index

5

Scholar i10-index

Scopus Publications

  • Detection and Utilisation of KASPar SNP Marker Assays for Early Blight Resistance in Tomato
    Ramesh Kumar Singh, Major Singh, Satyendra Pratap Singh, Vikas Kumar Singh, Neeraj Dwivedi
    Current Microbiology, 2026
  • Hybrid performance, combining ability and inheritance of quantitative and quality traits including shelf life in tomato (Solanum lycopersicum L.)
    Rashmi Singh, Nagendra Rai, Prashant Kumar, Dhananjay K. Upadhyay, Neeraj Dwivedi, Ramesh K. Singh
    Euphytica, 2025
  • High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas
    Shiwani Singh, Maneesh S. Bhandari, Neeraj Dwivedi
    Journal of Medicinal Plants for Economic Development, 2025
    Background: Desmodium gangeticum, a crucial medicinal shrub from the Himalayan Shivalik region, is an economically important species globally. It contains a vast range of secondary metabolites, majorly polyphenols, that interfere with high-quality deoxyribonucleic acid (DNA) extraction by binding their oxidised form to DNA covalently and rendering it unusable for molecular research.Aim: This study was focused on developing a protocol for the isolation of high-quality DNA from D. gangeticum, which is essential for carrying out molecular biological experiments. Also, the isolation of DNA in purified form has its significance in facilitating precise genetic analysis such as sequencing, restriction digestion and polymerase chain reaction (PCR)-based trials.Setting: This study was conducted under controlled laboratory environment at Manav Rachna Centre for Medicinal Plant Pathology, Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad, India.Methods: Advanced techniques, commercial kits and rigorous modifications in existing DNA extraction buffer conditions and extraction protocols from both fresh and dried leaves are considered to optimise high-quality DNA isolation.Results: With the standardised buffer conditions and protocol, high-quality DNA with purity indicated by a 260/280 nm absorbance ratio in a range of 1.8 to 2.0 with 250 ng/µL – 950 ng/µL concentration from 0.5 g of leaf sample and also validated by agarose gel electrophoresis showing reduced contaminations and intact DNA was isolated.Conclusion: This standardised buffer condition and protocol led to high-quality DNA isolation, which was found good for restriction digestion using endonuclease enzymes, PCR and other molecular biology techniques, highlighting the ongoing efforts to enhance the reliability of genetic studies in medicinal plants.Contribution: This study adds to the existing knowledge about the strategies and procedures for extracting high-quality DNA and good yield from leaf samples of D. gangeticum.
  • Genetic analysis and QTLs identification for resistance to the Begomovirus causing pepper leaf curl virus (PepLCV) disease
    Neeraj Dwivedi, Madhvi Mishra, Shyam Sundar Sharma, Ramesh Kumar Singh
    Journal of Plant Biochemistry and Biotechnology, 2024
  • Fly Ash Utilization: A Step Towards Sustainable Development
    Sadhana Shukla, Nidhi Didwania, Pushpa, Shiwani, Neeraj Dwivedi
    2024 1st International Conference on Sustainability and Technological Advancements in Engineering Domain Sustained 2024, 2024
    Fly ash is generated as a by-product of coal combustion and recently this getting recognition as a sustainable material in various sectors. It has good richness in nutrients, thus suitable for agriculture, and in strength it is best for constructing activities. Heavy metals are found in fly ash causes challenges, particularly in concrete durability and stability, leaching risks, harmful effect on soil health, crop development and productivity, and the residues of heavy metals in agricultural systems. It is required to develop necessary regulatory frameworks and essential management strategies to rectify these issues. This review highlights the potential usage of fly ash to enhance soil health, increase crop yields, and support the rehabilitation of degraded landscapes, and it also aligning with the multiple Sustainable Developments Goals (SDGs) like SDG 15 (Life on Land) and SDG 12 (Responsible Consumption and Production) by enhancing eco-friendly construction approaches. Moreover, it saves on carbon emissions as a medium of fly ash, thus helping SDG 13: Climate Action. More investigation is needed to track the durations of how fly ash impacts differently across sectors for understanding environmental risks as well as for advancing sustainable development.
  • Clay-Nanocomposite Based Smart Delivery Systems: A Promising Tool for Sustainable Farming
    Anamika Pal, Pawan Kaur, Neeraj Dwivedi, Jim Rookes, Himadri B. Bohidar, Wenrong Yang, David Miles Cahill, Palash Kumar Manna
    ACS Agricultural Science and Technology, 2023
    Clay-nanocomposite materials have been widely exploited as drug delivery systems, but their application in agriculture is less explored. In clay-nanocomposite, the combination of nanoclay and the active ingredient (i.e., metal, nonmetal, oxide nanoparticles) can produce a biocompatible and environmentally friendly delivery vehicle of micronutrients, pesticides, etc. for better crop growth. Clay-nanocomposites have been researched to address the problem of nontarget effects of agrochemicals, their leaching into groundwater, and the fast breakdown of sprayed chemicals. Here, we present a review of the synthesis of clay-based nanocomposites and their application as a delivery vehicle for agrochemicals and nucleic acids, as well as their antimicrobial properties. This review would be helpful in identifying and addressing the current issues of the delivery of active ingredients and biological macromolecules for crop protection, improved growth, and sustainability.
  • Evaluation of biochemical responses in pepper genotypes inoculated with Pepper leaf curl virus
    Neeraj Dwivedi, Leena Johny, Mayurika Goel, Dipti S. Tirkey
    Indian Phytopathology, 2022
  • Phylogenetic diversity and antioxidant activity of selected fungi from ethno-medicinal plants and soil
    Rahul Chandra Mishra, Colin J. Barrow, Rishu Kalra, Neeraj Dwivedi, Sunil K. Deshmukh, Mayurika Goel
    Mycological Progress, 2022
  • Evaluation of resistance against anthracnose (Colletotrichum capsici and C. gloeosporioides) in chilli landraces collected from the northeastern region of India
    Neeraj Dwivedi, Dipti S Tirkey, Shabnam Katoch, Lakshman Prasad
    Plant Genetic Resources Characterisation and Utilisation, 2021
    Anthracnose or fruit rot disease caused byColletotrichumspp. leads to substantial economic losses in chilli (Capsicum annuumL.) production worldwide. In the present study, 24 different Bhut Jolokia chilli landraces and sevenCapsicum annuumcultivars have been collected from the northeastern region of India and subsequently screened underin vitroandin vivoconditions againstColletotrichum capsiciandC. gloeosporiodesinfections. During field evaluation, eight chilli genotypes (CC0164, CC0165, CC0191, CC0192, CC0202, CC0206, CC0209 and CC0218) were highly resistant and 12 genotypes (CC0154, CC0179, CC0181, CC0183, CC0186, CC0189, CC0193, CC0198, CC0205, CC0210, CC0213 and CC0217) were found in resistant category againstC. capsiciinfection. Duringin-vitrogermplasm evaluation, 11 and 12 landraces were found to be highly resistant toC. capsiciandC. gloeosporioidesinfections, respectively. According to the findings, the majority of Bhut Jolokia chilli landraces are resistant to anthracnose. Given the difficulties farmers experience as a result of excessive use of fungicides and pesticides, germplasm screening for host resistance has already begun. The resistant lines identified in the current study offers better choices to tackle anthracnose and could be used effectively in breeding programs to develop anthracnose resistant varieties.
  • Keratinophilic fungi from the feathers of Emu (Dromaius novaehollandiae) in Maharastra India
    Sunil K. Deshmukh, Neeraj Dwivedi, Shilpa Amit Verekar
    Journal of Medical Mycology, 2021
  • QTL mapping for important horticultural traits in pepper (Capsicum annuum L.)
    Neeraj Dwivedi, Rajesh Kumar, Rajneesh Paliwal, Uttam Kumar, Sanjeet Kumar, Major Singh, Rakesh Kumar Singh
    Journal of Plant Biochemistry and Biotechnology, 2015
  • QTL analysis associated with oleoresin content in intraspecific RIL population of Chilli (Capsicum annuum L.)
    Journal of Applied Horticulture, 2014
  • Genetic variability among Jatropha species as revealed by amplified fragment length polymorphism (AFLP) markers
    Indian Journal of Biotechnology, 2014
  • Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum
    Rajesh Kumar, Sanjay Kumar, Neeraj Dwivedi, Sanjeet Kumar, Ashutosh Rai, Major Singh, Dasrath Singh Yadav, Mathura Rai
    Scientia Horticulturae, 2009

RECENT SCHOLAR PUBLICATIONS

  • Genetic analysis and QTLs identification for resistance to the Begomovirus causing pepper leaf curl virus (PepLCV) disease
    N Dwivedi, M Mishra, SS Sharma, RK Singh
    Journal of Plant Biochemistry and Biotechnology 33 (1), 34-44 , 2024
    2024
    Citations: 8
  • Interpretation for genetic nature of morphological traits and phyto-physio-chemical properties in tomato: An overview
    RK Singh, SS Solankey, N Dwivedi, DK Upadhyay, PK Singh, VK Singh, ...
    Journal of Pharmacognosy and Phytochemistry 13 (2), 114-127 , 2024
    2024
    Citations: 2
  • Clay-nanocomposite based smart delivery systems: A promising tool for sustainable farming
    A Pal, P Kaur, N Dwivedi, J Rookes, HB Bohidar, W Yang, DM Cahill, ...
    ACS Agricultural Science & Technology 3 (1), 3-16 , 2022
    2022
    Citations: 22
  • Evaluation of biochemical responses in pepper genotypes inoculated with Pepper leaf curl virus
    N Dwivedi, L Johny, M Goel, DS Tirkey
    Indian Phytopathology 75 (4), 1151-1157 , 2022
    2022
    Citations: 1
  • Phylogenetic diversity and antioxidant activity of selected fungi from ethno-medicinal plants and soil
    RC Mishra, CJ Barrow, R Kalra, N Dwivedi, SK Deshmukh, M Goel
    Mycological Progress 21 (2), 33 , 2022
    2022
    Citations: 6
  • Evaluation of resistance against anthracnose (Colletotrichum capsici and C. gloeosporioides) in chilli landraces collected from the northeastern region of India
    N Dwivedi, DS Tirkey, S Katoch, L Prasad
    Plant Genetic Resources 19 (6), 538-544 , 2021
    2021
    Citations: 3
  • Keratinophilic fungi from the feathers of Emu (Dromaius novaehollandiae) in Maharastra India
    SK Deshmukh, N Dwivedi, SA Verekar
    Journal of Medical Mycology 31 (2), 101133 , 2021
    2021
    Citations: 7
  • Triple-smart eco-friendly chili anthracnose control agro-nanocarrier
    S Sharma, B Singh, P Bindra, P Panneerselvam, N Dwivedi, A Senapati, ...
    ACS applied materials & interfaces 13 (7), 9143-9155 , 2021
    2021
    Citations: 41
  • QTL mapping for important horticultural traits in pepper ( Capsicum annuum L.).
    ND Neeraj Dwivedi, RK Rajesh Kumar, RP Rajneesh Paliwal, ...
    2015
  • QTL mapping for important horticultural traits in pepper ( Capsicum annuum L.)
    N Dwivedi, R Kumar, R Paliwal, U Kumar, S Kumar, M Singh, RK Singh
    Journal of Plant Biochemistry and Biotechnology 24 (2), 154-160 , 2015
    2015
    Citations: 53
  • Genetic variability among Jatropha species as revealed by amplified fragment length polymorphism (AFLP) markers
    P Sinha, N Dwivedi, MS Negi, SB Tripathi
    Indian J Biotechnol 13, 496-501 , 2014
    2014
    Citations: 4
  • QTL analysis associated with oleoresin content in intraspecific RIL population of chilli (Capsicum annuum L.)
    N Dwivedi, R Kumar, RK Singh, M Singh
    Journal of Applied Horticulture 16 (2), 107-111 , 2014
    2014
    Citations: 5
  • Genetic variability among Jatropha species as revealed by amplified fragment length polymorphism (AFLP) markers.
    PS Pratima Sinha, ND Neeraj Dwivedi, MS Negi, SB Tripathi
    2014
  • QTL analysis associated with oleoresin content in intraspecific RIL population of chilli ( Capsicum annuum L.).
    ND Neeraj Dwivedi, RK Rajesh Kumar, RK Singh, MS Major Singh
    2014
  • A review on molecular characterization of pepper for capsaicin and oleoresin.
    RK Rajesh Kumar, ND Neeraj Dwivedi, RK Singh, SK Sanjay Kumar, ...
    2011
    Citations: 50
  • Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum .
    RK Rajesh Kumar, SK Sanjay Kumar, ND Neeraj Dwivedi, ...
    2009
  • Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum
    R Kumar, S Kumar, N Dwivedi, S Kumar, A Rai, M Singh, DS Yadav, ...
    Scientia Horticulturae 120 (2), 167-172 , 2009
    2009
    Citations: 35

MOST CITED SCHOLAR PUBLICATIONS

  • QTL mapping for important horticultural traits in pepper ( Capsicum annuum L.)
    N Dwivedi, R Kumar, R Paliwal, U Kumar, S Kumar, M Singh, RK Singh
    Journal of Plant Biochemistry and Biotechnology 24 (2), 154-160 , 2015
    2015
    Citations: 53
  • A review on molecular characterization of pepper for capsaicin and oleoresin.
    RK Rajesh Kumar, ND Neeraj Dwivedi, RK Singh, SK Sanjay Kumar, ...
    2011
    Citations: 50
  • Triple-smart eco-friendly chili anthracnose control agro-nanocarrier
    S Sharma, B Singh, P Bindra, P Panneerselvam, N Dwivedi, A Senapati, ...
    ACS applied materials & interfaces 13 (7), 9143-9155 , 2021
    2021
    Citations: 41
  • Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum
    R Kumar, S Kumar, N Dwivedi, S Kumar, A Rai, M Singh, DS Yadav, ...
    Scientia Horticulturae 120 (2), 167-172 , 2009
    2009
    Citations: 35
  • Clay-nanocomposite based smart delivery systems: A promising tool for sustainable farming
    A Pal, P Kaur, N Dwivedi, J Rookes, HB Bohidar, W Yang, DM Cahill, ...
    ACS Agricultural Science & Technology 3 (1), 3-16 , 2022
    2022
    Citations: 22
  • Genetic analysis and QTLs identification for resistance to the Begomovirus causing pepper leaf curl virus (PepLCV) disease
    N Dwivedi, M Mishra, SS Sharma, RK Singh
    Journal of Plant Biochemistry and Biotechnology 33 (1), 34-44 , 2024
    2024
    Citations: 8
  • Keratinophilic fungi from the feathers of Emu (Dromaius novaehollandiae) in Maharastra India
    SK Deshmukh, N Dwivedi, SA Verekar
    Journal of Medical Mycology 31 (2), 101133 , 2021
    2021
    Citations: 7
  • Phylogenetic diversity and antioxidant activity of selected fungi from ethno-medicinal plants and soil
    RC Mishra, CJ Barrow, R Kalra, N Dwivedi, SK Deshmukh, M Goel
    Mycological Progress 21 (2), 33 , 2022
    2022
    Citations: 6
  • QTL analysis associated with oleoresin content in intraspecific RIL population of chilli (Capsicum annuum L.)
    N Dwivedi, R Kumar, RK Singh, M Singh
    Journal of Applied Horticulture 16 (2), 107-111 , 2014
    2014
    Citations: 5
  • Genetic variability among Jatropha species as revealed by amplified fragment length polymorphism (AFLP) markers
    P Sinha, N Dwivedi, MS Negi, SB Tripathi
    Indian J Biotechnol 13, 496-501 , 2014
    2014
    Citations: 4
  • Evaluation of resistance against anthracnose (Colletotrichum capsici and C. gloeosporioides) in chilli landraces collected from the northeastern region of India
    N Dwivedi, DS Tirkey, S Katoch, L Prasad
    Plant Genetic Resources 19 (6), 538-544 , 2021
    2021
    Citations: 3
  • Interpretation for genetic nature of morphological traits and phyto-physio-chemical properties in tomato: An overview
    RK Singh, SS Solankey, N Dwivedi, DK Upadhyay, PK Singh, VK Singh, ...
    Journal of Pharmacognosy and Phytochemistry 13 (2), 114-127 , 2024
    2024
    Citations: 2
  • Evaluation of biochemical responses in pepper genotypes inoculated with Pepper leaf curl virus
    N Dwivedi, L Johny, M Goel, DS Tirkey
    Indian Phytopathology 75 (4), 1151-1157 , 2022
    2022
    Citations: 1
  • QTL mapping for important horticultural traits in pepper ( Capsicum annuum L.).
    ND Neeraj Dwivedi, RK Rajesh Kumar, RP Rajneesh Paliwal, ...
    2015
  • Genetic variability among Jatropha species as revealed by amplified fragment length polymorphism (AFLP) markers.
    PS Pratima Sinha, ND Neeraj Dwivedi, MS Negi, SB Tripathi
    2014
  • QTL analysis associated with oleoresin content in intraspecific RIL population of chilli ( Capsicum annuum L.).
    ND Neeraj Dwivedi, RK Rajesh Kumar, RK Singh, MS Major Singh
    2014
  • Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum .
    RK Rajesh Kumar, SK Sanjay Kumar, ND Neeraj Dwivedi, ...
    2009