UNDERGRADUATE- BIOLOGY - FACULTY OF SCIENCE
MASTER'S DEGREE - HISTOLOGY - FACULTY OF VETERINARY MEDICINE
DOCTORATE - ANATOMY - FACULTY OF VETERINARY MEDICINE
Determination of Embryotoxic Dose Limit of Doxycycline and Its Effects on Some Long Bones by In Ovo Model Mustafa Sedat Arslan, Selvinaz Öztürk, Reyhan Rabia Kök, Kamil Beşoluk, Mustafa Orhun Dayan Journal of Veterinary Medicine Series C Anatomia Histologia Embryologia, 2025 The aim of this study was to determine the embryotoxic and teratogenic effects of doxycycline on chicken embryos. Eggs placed in the incubator were checked with an ovoscope on the 7th day, and a total of 210 fertile chicken eggs were randomly divided into 7 groups. Doxycycline was applied to the experimental groups at 2500, 1250, 625, 312, and 156 μg/50 μL volumes, respectively. Group 6 was evaluated as the Negative Control Group. This group was placed in the incubator again without any treatment. Group 7 was administered 50 μL of 0.9% NaCl solution used as the solvent of the drug. The number of live and dead embryos was determined after the completion of incubation. Mortality was observed depending on the dose, and death was determined in all chicks at the highest dose (2500 μg/50 μL, p < 0.05). No macroscopic anomalies were observed. While a decrease in mortality rates was observed depending on the dose (p < 0.05), the mortality rate in the 156 μg/50 μL dose (Group 5) was similar to the Positive and Negative control groups (p > 0.05). Femur and tibia parameters could not be measured because all embryos in Group 1 (2500 μg/50 μL) died. The length and thickness of the right and left femur and tibia in Group 2 (1250 μg/50 μL) were statistically significantly (p < 0.05) lower than those of the other groups. As a result, it can be stated that doxycycline use should be avoided in pregnant mammals, especially during the development phase of organs, unless necessary.
The embryotoxic and teratogenic effects of metamizole sodium Selvinaz Öztürk, Mustafa Orhun Dayan Journal of Veterinary Medicine Series C Anatomia Histologia Embryologia, 2024 Drug use during pregnancy is an important issue that must be investigated due to its adverse effects on maternal and foetal health. This study aimed to determine the embryotoxic and teratogenic effects of in‐ovo administered metamizole (dipyrone), which can be used when needed during pregnancy and has potent analgesic, antipyretic, anti‐inflammatory, and long bone (tibia and femur) effects. This study used 240 fertile eggs from Atak S breed chickens, divided into eight equal groups: control, vehicle control, and 15.62, 31.25, 62.5, 125, 250 and 500 mg/kg metamizole. The eggs were hatched on the 21st day of incubation, and the chicks' body weights and mortality rates were determined. The right and left femur and tibia bones were resected from the chicks. Anatomical reference points were determined after removing the soft tissues of the bones, and necessary morphometric measures were taken from these points with a 0.01 mm precision using digital callipers. The 100% lethal dose (LD100) was identified in the highest examined dose (500 mg/kg) in the Chicken Embryotoxicity Screening Test (CHEST)‐I stage. The CHEST‐II stage determined the 50% lethal dose (LD50). High‐dose metamizole affected skeletal development, significantly decreasing tibia and femur lengths and corpus thicknesses and increasing mortality.
RECENT SCHOLAR PUBLICATIONS
Determination of Embryotoxic Dose Limit of Doxycycline and Its Effects on Some Long Bones by In Ovo Model MS Arslan, S Öztürk, RR Kök, K Beşoluk, MO Dayan Anatomia, Histologia, Embryologia 54 (2), e70030 , 2025 2025 Citations: 5
The embryotoxic and teratogenic effects of metamizole sodium S Öztürk, MO Dayan Anatomia, Histologia, Embryologia 53 (5), e13101 , 2024 2024 Citations: 5
Metamizol’ün Embriyotoksik ve Teratojenik Etkilerinin Belirlenmesi S Öztürk Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü , 2023 2023
The effects of bisphenol A given in ovo on bursa of Fabricius development and percentage of acid phosphatase positive lymphocyte in chicken S Çetin, T Özaydın Environmental Science and Pollution Research 28 (31), 41688-41697 , 2021 2021 Citations: 14
MOST CITED SCHOLAR PUBLICATIONS
The effects of bisphenol A given in ovo on bursa of Fabricius development and percentage of acid phosphatase positive lymphocyte in chicken S Çetin, T Özaydın Environmental Science and Pollution Research 28 (31), 41688-41697 , 2021 2021 Citations: 14
Determination of Embryotoxic Dose Limit of Doxycycline and Its Effects on Some Long Bones by In Ovo Model MS Arslan, S Öztürk, RR Kök, K Beşoluk, MO Dayan Anatomia, Histologia, Embryologia 54 (2), e70030 , 2025 2025 Citations: 5
The embryotoxic and teratogenic effects of metamizole sodium S Öztürk, MO Dayan Anatomia, Histologia, Embryologia 53 (5), e13101 , 2024 2024 Citations: 5
Metamizol’ün Embriyotoksik ve Teratojenik Etkilerinin Belirlenmesi S Öztürk Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü , 2023 2023
