Agronomy and Crop Science, Horticulture, Agricultural and Biological Sciences, Plant Science
75
Scopus Publications
Scopus Publications
Chemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa Luís Felipe Rodrigues Costa, Amanda Evelyn Miranda, Márliton Pereira dos Santos, Jorge Luiz Soares dos Anjos, Fernanda Menezes Maia, Tiara da Costa Silva, Mario Machado Martins, Joseilton Faria Silva, Isac Pereira Soares Martins, Clarice Diniz Alvarenga, Marlon Cristian Toledo Pereira, Nelson de Abreu Delvaux Júnior, Luciano Pereira Rodrigues Applied Microbiology and Biotechnology, 2025 The growing demand for sustainable agricultural pest control solutions has increased the use of microbial bioinput. This study characterized an industrial bioinput produced by Saccharopolyspora spinosa , focusing on identifying metabolites and quantifying spinosyns A and D by HPLC-MS. The concentrations of these compounds were 29.4 ± 3.6 mg L −1 and 13.3 ± 1.5 mg L −1 , respectively, for a total of 42.7 mg L −1 . The limit of quantification (LOQ) and limit of detection (LOD) values obtained were 13.7 ng L −1 and 4.16 ng L −1 for spinosyn A and 7.63 ng L −1 and 2.31 ng L −1 for spinosyn D, respectively. The bioinput contained essential amino acids (leucine, phenylalanine, and tryptophan), plant cell death inducers (sphinganine), diketopiperazines with insecticidal potential (Cyclo(Met-Val)), larvicides ( N -stearoyl tryptophan), antimicrobials (Penicitrinol D), and phospholipids associated with cell defense and stress responses (phosphatidylethanolamine, phosphatidylserine, ceramides, and mycolactones). In vitro trials demonstrated mortalities of 82.5% for Spodoptera frugiperda , 100.0% for Dalbulus maidis , and 64.1% for Ceratitis capitata . These results are statistically equivalent to those obtained using commercial products, which cost up to five times more. This bioinput’s chemical diversity suggests multifunctional action, probable synergism, and a lower risk of resistance, which reinforces its potential in biological agricultural management. Key points • Identification of a diverse set of bioactive compounds, including spinosyns A and D. • Bioinput achieves statistical efficacy equal to commercial products in in vitro tests. • A sustainable, cost-effective alternative for large-scale production and application.
Selection Strategy for Breeding Pepper Lines with Ornamental Potential Fátima de Souza Gomes, Samy Pimenta, Gabriela Cristina Alves Custódio, Wellington Silva Gomes, Joyce Costa Ribeiro, Nelson de Abreu Delvaux Júnior, Marlon Cristian Toledo Pereira, Monique Moreira Moulin, Willer Fagundes de Oliveira, Ana Karolyne Pereira Barbosa, Hélida Christhine de Freitas Monteiro, Ana Carolina Petri Gonçalves, Marcos Vinicius Bohrer Monteiro Siqueira Horticulturae, 2025 Considering that effective selection strategies are essential for the development of new ornamental pepper cultivars, the objective of this work was to select superior partially endogamic lines (PEL) of pepper in a F2:3 generation, using the combination of the genealogical method with mixed linear models. The experiment consisted of four cycles: parents and generations F1, F2 and F2:3. Qualitative (QLD) and quantitative (QTD) descriptors were evaluated. QLD were analyzed through descriptive statistics, and QTD were analyzed through estimates of genetic parameters and prediction of genetic values by REML/BLUP. Multivariate analysis was performed to group and select individuals based on QLD and QTD simultaneously. The descriptors number of flowers per axil, flower position, mature fruit color, fruit position, fruit brightness, and capsaicin in the placenta presented no variation within the F2:3 population. The selection accuracy varied from high to very high, denoting a high experimental precision. Higher additive genetic action was detected for descriptors, considering the individual heritability in the strict sense and the additive heritability within the progeny. Forty-eight PELs were selected quantitatively and, considering QLD and QTD descriptors simultaneously, the number of individuals was reduced from 48 to 30 PELs. The combined strategies used enabled to establish the best strategy for an efficient selection of superior PEL of ornamental pepper.
‘Prata Anã’ banana under application of paclobutrazol on soil and 2,4-D in the inflorescence in three production cycles: multivariate approach Leonardo Carvalho Brant Maia, Victor Martins Maia, Alcinei Mistico Azevedo, Evander Alves Ferreira, Sérgio Luiz Rodrigues Donato, Marlon Cristian Toledo Pereira Frontiers in Agronomy, 2025 Paclobutrazol (PBZ) inhibits gibberellin biosynthesis and reduces plant size, whereas 2,4-D (2,4 dichlorophenoxyacetic acid) causes cell elongation and enhances fruit growth and productivity. In this study, we used a multivariate approach to evaluate the production and post-harvest characteristics of the ‘Prata-Anã’ banana in three production cycles after the application of paclobutrazol in the soil and 2,4-D during flowering. A randomized block design (4×4 factorial arrangement) was used, referring to the paclobutrazol (0, 0.1, 0.3, 0.5 g L-1) and 2,4-D concentrations (0, 10, 20, and 30 mg L-1), with three replicates. Five vegetative characteristics and one physiological characteristic were evaluated during plant flowering. Nine production variables were evaluated during harvest. The values of five production variables and two vegetative growth variables increased and the number of days between the clústeres decreased as the cycles progressed. 2,4-D did not affect the fruit production-associated characteristics. Further, 0.5 g a.i. (active ingredient), PBZ yielded lower and higher values for the production variables in the first and second production cycles, respectively. In the third cycle, 0.1 g a. i. paclobutrazol exerted the most prominent effect on the production variables, indicating that the plant response to growth regulators changes according to the production cycle.
Kenworthy and DRIS norms for ‘Prata-Anã’ and ‘BRS Platina’ banana plants in improved fertility soils Márcio Adriano Santos, Sérgio Luiz Rodrigues Donato, Júlio César Lima Neves, Pedro Ricardo Rocha Marques, Marlon Cristian Toledo Pereira, Maria Geralda Vilela Rodrigues Nativa, 2025 The objective was to establish and evaluate the similarity of the Kenworthy and DRIS norms for ‘Prata’ banana in soils with improved fertility. The data used were leaf nutrient contents and yields of 180 plots, from an experiment with two cultivars (‘Prata-Anã’ and ‘BRS Platina’), five doses of K2O (0; 200; 400; 600 and 800 kg ha-1) supplied with sources for organic management and six evaluation times (210; 390; 570; 750; 930 and 1,110 days after planting), in a randomized block design in factorial scheme, with three replicates. The database was separated from the average yield, 36.42 t ha-1 cycle-1 for ‘Prata-Anã’ and 38.4 t ha-1 cycle-1 for ‘BRS Platina’, into low-yielding population (LYP) and high-yielding population (HYP). Mean, standard deviation, coefficient of variation, variance and variance ratio (HYP/LYP) of nutrients and their bivariate ratios, direct (A/B) and inverse (B/A), were calculated. ‘Prata-Anã’ and ‘BRS Platina’ in HYP differed in terms of the means and variances for Kenworthy by 33.00%, for the DRIS, the means differed in 71.21% and variances in 43.18% of the cases. The Kenworthy and DRIS norms established support the nutritional diagnosis of ‘Prata-Anã’ and ‘BRS Platina’ banana plants grown in soils with improved fertility.
Genetic inheritance of ornamental components in pepper plants (Capsicum annuum L.) Fátima de Souza Gomes, Gabriela Cristina Alves Custódio, Samy Pimenta, Fabrícia Cardoso Oliveira, Allyson Gabriel Santos de Paula, et al. Acta Scientiarum Agronomy, 2025 Limited information is available regarding the genetic inheritance of ornamental traits in peppers (Capsicum spp.), which is crucial for enhancing these plants for ornamental purposes in breeding programs. This study aimed to elucidate the genetic inheritance of ornamental traits in segregating populations of pepper plants (C. annuum L.) from distinct parents and to characterize them based on their flowering and fruiting cycles. The selected parents, UNI01 and UNI05, were sourced from the active germplasm bank of Universidade Estadual de Montes Claros, Janaúba, Minas Gerais State, Brazil. The experiment took place in a greenhouse, involving manual hybridization between UNI01 and UNI05 to obtain seeds of segregating populations, which included F1, RC1, RC2, F2, and F3 generations. Qualitative traits assessed included flower corolla color, immature fruit color, and shapes of longitudinal and transversal fruit sections. Quantitative traits encompassed mean flowering and fruiting days. We employed chi-square tests (χ²) to evaluate segregation patterns. The descriptor "corolla color" exhibited codominance, with a white corolla and purple borders linked to heterozygous genotypes. Dominant inheritance controlled the color of immature fruits, primarily purple. Genetic inheritance for transversal and longitudinal fruit shapes remained unexplained in the tested segregations. Parental and segregating generations displayed similar flowering and fruiting cycles. These results provide valuable insights for future breeding programs aimed at using this species for ornamental purposes.
Selection of clones and parents in full-sibling families of Jatropha curcas Joseilton Faria Silva, Silvia Nietsche, Samy Pimenta, Marlon Cristian Toledo Pereira, Alcinei Místico Azevedo, Renata Aparecida Neres Faria, Joseane Faria da Silva Souza, Gabriela Cristina Alves Custódio Pesquisa Agropecuaria Brasileira, 2025 The objective of this work was to estimate some genetic parameters of Jatropha curcas, in order to predict which productive characteristics could show the greatest genetic gain to identify which family would contribute the most to the selection of clones and parents, and to identify the best genotype for selection. Six full-sibling families with 18 individuals each were evaluated. Five production traits were evaluated in the third year after planting. Data obtained were analyzed through mixed models. The restricted maximum likelihood (REML) and the best linear unbiased prediction (BLUP) procedures were used to estimate the genetic parameters and to predict the selection gains, respectively. Environmental effects predominated over genetic effects for most evaluated traits. A hundred-seed press cake weight showed high additive heritability estimates. The BLUP analysis individual components, in the selection of the best progenies for use as potentials parents, showed significantly similar values to those of the selection of the best progenies for use as clones. The family F is the most relevant in the selection of potential parents and clones by the evaluated characters. The F9 progeny is the best individual for use as a parent and for vegetative propagation (clone).
Genome sequencing of Bacillus cereus isolate EB-40: a plant growth-promoting bacterium Débora Francine Gomes Silva Pereira, Pedro Marcus Pereira Vidigal, Samuel A. Santos, Silvia Nietsche, Adelica Aparecida Xavier, Marlon Cristian Toledo Pereira Discover Applied Sciences, 2024 Bacillus sp. isolate EB-40 was characterized in 'Prata Anã' banana (Musa sp.) plants as an endophyte capable of colonizing both the inter- and intracellular spaces of roots, nitrogen fixation, phosphate solubilization, in vitro synthesis of indole-3-acetic acid, and promotion of the development of micropropagated banana seedlings. Here, we report the whole-genome sequence of Bacillus sp. isolate EB-40 and its taxonomic assignment. Its genome is composed of one chromosome and three plasmids. The circular double-stranded DNA chromosome (5,613,235 base pairs (bp)) has a GC content of 35.3% and 5462 genes. The three plasmids have a total length of 237,685 bp with 201 genes. Comparative genomics revealed that its genome shares similarity indices with Bacillus cereus genomes above the thresholds recommended for species assignment.
Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions Bruno Rafael Alves Rodrigues, Charles S. Gasser, Samy Pimenta, Marlon Cristian Toledo Pereira, Silvia Nietsche Plant Reproduction, 2024 Key message Inheritance of the presence/absence of seeds in Annona squamosa is mediated by a single fully recessive gene and is caused by a deletion of the INNER NO OUTER (INO) locus. Abstract For some fruits, seedless varieties are desirable for consumption and processing. In the sugar apple tree (Annona squamosa L.), the seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with defective ovules and an apparent deletion of the INNER NO OUTER (INO) ovule development gene locus. Segregation analysis of F2 and backcross descendants of crosses of Bs to fertile wild-type varieties in this species with a multi-year generation time showed that seedlessness was recessive and controlled by a single locus. Comparison of whole genome sequence of a wild-type plant and a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO in this line. Ts and Bs lines were shown to have an identical deletion, indicating a common origin from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three seedless accessions are vegetatively propagated clones. The sequence of the deletion site enabled a codominant assay for the wild-type and mutant genes allowing observation of complete cosegregation of the seedless/defective ovule phenotype with the INO deletion, showing maximal separation of less than 3.5 cM. The observed deletion is the only significant difference between the wild-type and Hs line over 587 kilobases, likely encompassing much more than 3.5 cM, showing that the deletion is the cause of seedless trait. The codominant markers and obtained progenies will be useful for introgression of the seedless trait into elite sugar apple lines and into other Annonas through interspecific crossings.
Selection of ideal genotypes in peppers with ornamental potential Gabriela Cristina Alves Custódio, Samy Pimenta, Fátima de Souza Gomes, Nadiany S. Silva, Bruno Rafael Alves Rodrigues, Fabrícia Cardoso Oliveira, Nelson de Abreu Delvaux Júnior, Marlon Cristian Toledo Pereira Folia Horticulturae, 2023 Ornamental peppers have a significant economic importance in the national and international markets; however, few cultivars intended for this purpose are grown in Brazil. The objective of this study was to select partially inbred lines (PIL) of peppers with ornamental potential, based on quantitative and qualitative variables with high heritability. The study was conducted over six generations for 4 consecutive years, using the single seed descent method. The last phase (VI) consisted of growing plants of the F5 generation for selection. Qualitative (LD) and quantitative (QD) descriptors were considered and the ideal genotypes were defined. The statistical analyses consisted of estimating variance components and genetic parameters and predicting genetic values, using REML/BLUP for QD, except for cycle to flowering (CF) and cycle to maturation (CM), which were analysed qualitatively. Therefore, CF and CM were analysed through frequency distribution of continuous variables within class intervals. Descriptive statistics were used to evaluate LD. The results showed that residual values exceeded genetic values, resulting in low heritability for QD, and therefore, they were not considered for PIL selection. Regarding LD, genetic variability was found among the population genotypes for all evaluated descriptors. The selection based on ideal genotypes enabled the selection of 82 PIL with LD of high ornamental value, which differ from the materials already available on the market. The selected genotypes will be used for developing future generations until allele fixation, focussed on subsequent selection of candidate lines for new ornamental pepper cultivars.
Morphological characterization of new banana clones-elites Thaís Angélica de Souza Soares, Marlon Cristian Toledo Pereira, Lucicléia Borges de Almeida, Silvia Nietsche, Samy Pimenta, Bruno Rafael Alves Rodrigues Revista Brasileira De Fruticultura, 2023
Effect of amino acid on panicle length and fruit set of tree mangoes (cultivar tommy atkins) in semi arid of north of Minas Gerais Acta Horticulturae, 2009
Explants establishment in vitro of three banana cultivars Silvia Nietsche, Sandra Vanessa Marques, Marlon Cristian Toledo Pereira, Bárbara Salles, Adelica Aparecida Xavier, André Cabral França, Cynthia de Lima, Lucivânia Sanguinette Silva Ciencia Rural, 2006