Biotechnology, Agricultural and Biological Sciences, Soil Science, Molecular Biology
33
Scopus Publications
665
Scholar Citations
10
Scholar h-index
12
Scholar i10-index
Scopus Publications
Comparative Enzymatic and Gene Expression Responses in Wheat to DON- and NIV-Producing Fusarium Species Gabriela da Rocha Lemos Mendes, Paulo Cesar Pazdiora, Vivian Ebeling Viana, Leandro José Dallagnol, Laura Christina Calgaro, Glacy Jaqueline da Silva, Emerson Medeiros Del Ponte, Antônio Costa de Oliveira Biology, 2025 Fusarium head blight (FHB) is a major threat to wheat production that is caused by toxigenic species of the Fusarium graminearum complex. This study aimed to investigate the biochemical and molecular defense responses of Brazilian wheat genotypes (BRS 194, BRS Parrudo, and Frontana) with contrasting FHB susceptibilities following inoculation with F. graminearum (deoxynivalenol producer) and F. meridionale (nivalenol producer). Temporal patterns of antioxidant enzymes, defense-related enzymes, and gene expression (ABC-Transporter and Ca2+-ATPase) were analyzed from 12 to 96 h after inoculation. The ANOVA results revealed significant effects of genotypes, inoculation, and time after inoculation on most of the evaluated enzymatic activities. Frontana exhibited high basal activity for most enzymes, and after inoculation, the enzyme activity was higher than in other genotypes. BRS 194 presented delayed and fragmented activation patterns, particularly under DON-producing pathogen infection. According to the transcriptome results, inoculation with the NIV-producing pathogen upregulated both genes, reaching up to an 18-fold increase. BRS 194 showed an upregulated transcript pattern from the early hours after inoculation. Frontana showed increased transcript levels, reaching 12-fold, under DON-producing pathogen infection. These findings show that biochemical and molecular responses varied depending on genotype and the chemotype of the Fusarium isolate, highlighting the importance of early, coordinated defense activation in FHB resistance.
Comparative analysis of selected nutrients in Pereskia species J. D. Lima, R. F. Menegazzo, M. F. Silva, M. P. S. B. Barbosa, A. R. Schuelter, E. Jacomassi, G. J. Silva Brazilian Journal of Biology, 2025 This investigation undertakes a comparative quantitative assessment of the macronutrient and micronutrient profiles of Pereskia grandifolia (Haworth) and Pereskia aculeata (Miller), commonly known as Ora-pro-nóbis, categorized as Non-Conventional Food Plants (NCFPs), juxtaposing these profiles against established food crops. Quantitative analysis revealed that both Pereskia species exhibit elevated concentrations of micronutrients such as iron and zinc, surpassing those of Phaseolus vulgaris (Linnaeus) and Lens culinaris (Linnaeus). Specifically, solubilized zinc levels in P. aculeata (13.6 mg/100g) and P. grandifolia (13.9 mg/100g) exceed those of lentils (7.5 mg/100g) and beets (3.7 mg/100g). Similarly, solubilized iron levels in P. grandifolia (12.4 mg/100g) and P. aculeata (10.5 mg/100g) surpass those of lentils (3.0 mg/100g) and beets (3.8 mg/100g). However, phytate levels are comparatively lower in the Pereskia species, with P. grandifolia demonstrating reduced concentrations at 1500 mg/kg, alongside significant preponderance in protein (20.8 g/100g) and nitrogen (14.6 g/100g). Principal Component Analysis (PCA) was applied, demonstrating a strong associative propensity of iron, zinc, and protein with the Pereskia genus. In contrast, Pearson's correlation coefficients elucidated a complex interdependency among the nutrient variables, except for an inverse correlation between phytate concentration and both iron and protein levels. In conclusion, this research sheds light on the enriched nutritional profiles of P. grandifolia and P. aculeata, emphasizing their potential as valuable dietary resources.
Biological dynamics of no-tillage soils in the western region of Paraná J. D. Lima, W. R. Rivadavea, L. C. Calgaro, O. Alberton, M. S. S. M. Costa, J. C. Lima, P. H. R. Monteiro, E. V. Kuhn, G. J. Silva Brazilian Journal of Biology, 2025 This study investigates soil dynamics on farms in the western region of Paraná, Brazil, highlighting the importance of biological parameters in agriculture. In particular, focusing on the interaction of management practices with soil biodiversity and biological functions, the aim is to understand and promote sustainable and efficient agricultural practices. To do this, we collected soil samples from 15 farms close to Toledo, Paraná, Brazil. These samples were then analyzed to determine biological and physicochemical parameters using techniques such as carbon and nitrogen microbial biomass, metabolic coefficient, basal respiration, bacterial and fungal biomass, and length of the hyphae. The most contrasting soils were evaluated for physicochemical composition and metagenomic analyses. The results showed significant differences in biological parameters between 2020 and 2021, including fungal biomass, hyphae length, and soil basal respiration. Statistical analyzes revealed strong relationships between biological variables, notably the correlation between fungal hyphae and total nitrogen. Climate changes and management practices appear to influence the microbial composition and biological functions of the soil over the years. Soil P9 stood out with superior biological activity and richer microbial diversity, contrasting with soil P13. These differences reflect the influence of management and climatic conditions on soil composition and biological functions. The microbial comparison of the soils emphasized the need for continuous and careful agricultural management, highlighting the importance of biodiversity and ecological functionality of the soil for agricultural sustainability. So, the study underscores the relevance of considering soil biological parameters, in addition to physicochemical aspects, to optimize soil health and productivity.
Pseudomonas fluorescens in plant growth promotion and biocontrol: A focus on secondary metabolites, IAA, and siderophores Mayara dos Santos Queiroz, Luanna Paula Lopes, Pedro Bianchini, Renan Rissato Spessato, Glacy Jaqueline da Silva Australian Journal of Crop Science, 2024 Pseudomonas fluorescens, a Gram-negative bacterium abundant in soil, plays a critical role in promoting plant growth and controlling pathogens, demonstrating remarkable biocontrol capabilities. This review explores the utility of P. fluorescens and its ability to produce secondary metabolites, IAA (Indole-3-acetic acid), and siderophores, addressing agricultural challenges under the strains of climate change. It emphasizes its role as a plant growth-promoting bacterium (PGPB), synthesizing recent findings on its contributions to enhancing plant resilience, pathogen resistance, and sustainable agricultural practices. The production of secondary metabolites, IAA, and siderophores by P. fluorescens is examined for its effectiveness in biocontrol, nutrient mobilization, and hormonal regulation. These functions are critically analyzed through diverse research methodologies, including laboratory and field trials, underscoring the bacterium’s pivotal role in advancing agricultural sustainability and productivity. As the agricultural sector increasingly focuses on bio-products and the exploration of soil microorganisms, P. fluorescens emerges as a promising solution to enhance farming resilience in the face of climatic adversities.
Hydroponic effluent as an alternative fertilizer in bell pepper production Jhony Anderson Alvarenga Rios, Daisy Ramirez, Ernesto José Bernal Gini, Lucia Simeona Rios Valiente, Glacy Jaqueline Da Silva, Letícia Fernanda Giombelli Comunicata Scientiae, 2024 As hydroponic systems expand and generate effluents, research has increasingly focused on their management due to potential environmental impacts. This study examined the effectiveness of using hydroponic effluents as fertilizer in bell pepper cultivation. The chemical composition of the effluents was analyzed and compared against water quality standards set by Resolution No. 222/02 of SEAM. The study employed two application methods (foliar and root) and three concentrations (0%, 50%, 100%) of effluents. A randomized complete block design was used to evaluate plant height, fruit number, and yield. Results showed that a 50% foliar application significantly increased plant height compared to the 100% concentration, with no significant differences in fruit number and yield. For root application, no significant differences were observed across the variables at different concentrations, although the best performance was noted at 50%. Comparative analysis between application methods revealed that root application resulted in taller plants but lower productivity. The study concludes that using hydroponic effluents as fertilizers affects bell pepper productivity, with a 50% concentration being most effective regardless of the application method.
Expanding agricultural potential through biological nitrogen fixation: Recent advances and diversity of diazotrophic bacteria Julliane Destro de Lima, Adijailton José de Souza, Amanda Letícia Pit Nunes, Wesley Ribeiro Rivadavea, Geovanna Cristina Zaro, Glacy Jaqueline da Silva Australian Journal of Crop Science, 2024 Biological nitrogen fixation (BNF) by diazotrophic bacteria is one of the oldest and most crucial processes in nature. In this process, bacteria form symbiotic associations with plants, capturing atmospheric nitrogen and making it readily available to them. The diversity of nitrogen-fixing bacteria is vast. Recent advancements in molecular biology techniques have enabled the identification of new genera and species capable of fixing nitrogen and providing other types of nutrients for plants. From an agronomic perspective, this process is fundamental in increasing crop productivity sustainably and -cost-effectively. This review aims to categorize the most recent updates on the diversity of nitrogen-fixing bacteria and showcase the main advances in the genetic improvement of legumes for this characteristic. Recent research has revealed a wide diversity of species applicable to various crops of agronomic interest, and many of these bacteria have been used either alone or in consortium with other microorganisms. This study demonstrates the agricultural potential of these new discoveries and the vast possibilities for expanding research into the diversity of microorganisms responsible for BNF in agriculture.
Metagenomics and vegetative growth of Salvia hispanica inoculated with Trichoderma harzianum A. R. Schuelter, L. E. C. Pedron, G. J. da Silva, L. P. Bonett, I. R. P. de Souza, R. S. Grignet, J. V. G. Stathacos, A. F. Alves Netto, S. R. M. Coelho Brazilian Journal of Biology, 2024 The soil is a dynamic environment, influenced by abiotic and biotic factors, which can result in changes in plant development. This study aimed to assess the impact on vegetative growth of chia (Salvia hispanica L) inoculated with Trichoderma harzianum and on the rhizosphere microbiome. The experimentation was conducted in a greenhouse under controlled conditions growing chia plants in pots containing soil with a clayey texture. Different concentrations of T. harzianum (0; 2.5; 5.0; 10.0; 20.0 µL. g-1 of seed) were applied to the chia seeds before planting. Morphological parameters, including plant height (cm), number of branches, stem diameter (mm), number of days to flowering and shoot and root dry masses (g) were quantitatively assessed. After the cultivation period, soil samples from the rhizosphere region were collected for subsequent chemical and metagenomic analyses. These samples were also compared with the control soil, collected before installing the experiment. The results showed that increasing doses of T. harzianum promoted a significant increase in the diameter of the stem, number of branches, dry biomass of the root system and the number of days to flowering, without modifying the overall height of the plants. Soil metagenomics indicated that T. harzianum inoculation modified the microbial diversity of the rhizosphere environment, with more pronounced effects observed in samples treated with higher concentrations of the inoculant. Furthermore, there were changes in the chemical composition and enzymes related to soil quality in correlation with the concentrations of the applied inoculant. This study demonstrated that inoculating chia seeds with T. harzianum not only promotes specific morphogenetic characteristics of the plant, but it also has a significant impact on the microbial diversity and biochemical functionality of the soil, including an observed increase in the populations of T. harzianum and T. asperellum.
Impact of kinetin on essential oil content in Acmella oleracea Nastassja Kimberlly Lima, Eloisa Schneider da Silva, Rayane Monique Sete da Cruz, Julliane Destro de Lima, Cristian Coelho da Silva, Mayara dos Santos Queiroz, Zilda Cristiani Gazim, José Eduardo Gonçalves, Daisy Leticia Ramirez Monzon, Renan Rizzato Espessato, Glacy Jaqueline da Silva Plant Cell Tissue and Organ Culture, 2023
Microencapsulation of Saccharomyces boulardii using vegan and vegetarian wall materials Tárcio Enrico Savoldi, Fabiano Bisinella Scheufele, Deisy Alessandra Drunkler, Glacy Jaqueline da Silva, Juliana Destro de Lima, Keiti Lopes Maestre, Carina Contini Triques, Edson Antonio da Silva, Mônica Lady Fiorese Journal of Food Processing and Preservation, 2022
Influence of different preparation techniques on the composition and antioxidant action of curcumin and curcuminoids , Juliana Pelissari Marchi, Francislaine Aparecida dos Reis Livero, , Andreia Assuncao Soare, , Glacy Jaqueline da Silva, , Ana Karina Vargas Soares, , Camila Moreno Giarolo, , Ezilda Jacomassi, , Lauro Mera de Souza, , Wanessa de Campos Bortolucci, , Zilda Cristiani Gazim, , Caio Franco de Araujo Almeida Campos, , Jose Eduardo Goncalves, , Cintia de Souza Alferes Araujo, , Samantha Wietzikoski, , Evellyn Claudia Wietzikoski Lovato, and Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas, 2022
Microbial Enzymes and Soil Health Glacy Jaqueline da Silva, Wesley Ribeiro Rivadavea, Juliane Destro de Lima, Pedro Henrique Riboldi Monteiro, Franquiéle Bonilha da Silva Plant Growth Promoting Microbes for Sustainable Biotic and Abiotic Stress Management, 2021
Therapeutic potential of zingiberaceae in alzheimer's disease , Wanessa de Campos Bortolucci, Jessica Rezende Trettel, , Danilo Magnani Bernardi, , Marilñia Moraes Queiroz Souza, , Ana Daniela Lopes, , Evellyn C. W. Lovato, , Francislaine A. R. Livero, , Glacy J. da SilvaU, , Helida M. Magalhaes, , Silvia G. H. de Souza, , Zilda C. Gazim, , Nelson B. Colauto, and Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas, 2020
Comparative impact of organomineral and organic fertilizers as partial substitutes for synthetic fertilizers in enhancing soil fertility, health, and maize growth JC de Lima, EL Buligon, R Marostica, P Goufo, MSS de Mendonça Costa, ... Revista em Agronegócio e Meio Ambiente 19, e13332-e13332 , 2026 2026 Citations: 1
Comparative Enzymatic and Gene Expression Responses in Wheat to DON- and NIV-Producing Fusarium Species GRL Mendes, PC Pazdiora, VE Viana, LJ Dallagnol, LC Calgaro, ... Biology 14 (8), 1063 , 2025 2025 Citations: 2
Análise comparativa de nutrientes selecionados em espécies de Pereskia JD Lima, RF Menegazzo, MF Silva, M Barbosa, AR Schuelter, ... Brazilian Journal of Biology 85, e290533 , 2025 2025
Comparative analysis of selected nutrients in Pereskia species JD Lima, RF Menegazzo, MF Silva, M Barbosa, AR Schuelter, ... Brazilian Journal of Biology 85, e290533 , 2025 2025 Citations: 1
O Hydroponic effluent as an alternative Fertilizer in Bell Pepper Production JAA Rios, D Ramirez, EJB Gini, LSR Valiente, GJ da Silva, LF Giombelli Comunicata Scientiae 15, e4249-e4249 , 2024 2024
Expanding agricultural potential through biological nitrogen fixation: Recent advances and diversity of diazotrophic bacteria JD de Lima, AJ de Souza, ALP Nunes, WR Rivadavea, GC Zaro, ... Australian Journal of Crop Science 18 (6), 324-333 , 2024 2024 Citations: 15
Potential of endophytic bacteria from Acacia mearnsii: Phosphate solubilization, indole acetic acid production, and application in wheat JD de Lima, PHR Monteiro, WR Rivadavea, M Barbosa, RD Cordeiro, ... Applied Soil Ecology 196, 105315 , 2024 2024 Citations: 10
Progressive pollution abatement in raw dairy wastewater induced by the algae Poterioochromonas malhamensis with a high-value biomass yield AN Módenes, DG Fernandes, DEG Trigueros, MG Amador, ... Biochemical Engineering Journal 201, 109138 , 2024 2024 Citations: 4
Role of Pseudomonas fluorescens secondary metabolites in agroecosystem applications ALP Nunes, OF Perna, MS Queiroz, GC Zaro, JD de Lima, GJ da Silva Bacterial secondary Metabolites, 211-220 , 2024 2024 Citations: 2
Quantification of silicon concentration in sugarcane ash using the neutron activation technique with an isotopic source BJM Barboza, JC Cabello Leiva, DL Ramirez Monzon, LVB Chaparro, ... Australian Journal of Crop Science 17 (11), 862-866 , 2023 2023
Impact of kinetin on essential oil content in Acmella oleracea NK Lima, ES Da Silva, RMS Da Cruz, JD De Lima, CC Da Silva, ... Plant Cell, Tissue and Organ Culture (PCTOC) 153 (3), 539-546 , 2023 2023 Citations: 2
Climate Change and Its Implications for the Cultivation of Olive F Villa, DF da Silva, GJ da Silva Cultivation for Climate Change Resilience, Volume 2, 194-212 , 2023 2023
Climate change implications on cultivation of avocado (Persea americana Mill.) DF da Silva, F Villa, GJ da Silva Cultivation for Climate Change Resilience, Volume 1, 164-190 , 2023 2023 Citations: 4
Red LED light and different cultivation methods changed the essential oil composition of Acmella oleracea DB Dedino, JD de Lima, WC Bortolucci, WR Rivadavea, ECW Lovato, ... Plant Cell, Tissue and Organ Culture (PCTOC) 151 (3), 511-520 , 2022 2022 Citations: 3
Evaluation of water stress and co-inoculation of Azospirillum brasilense and Rhizobium tropici in beans (Phaseolus vulgaris L.). O Ferreira Perna, J Destro de Lima, W Ribeiro Rivadavea, ... Arquivos de Ciências Veterinárias e Zoologia da Unipar 25 (2) , 2022 2022 Citations: 1
Microencapsulation of Saccharomyces boulardii using vegan and vegetarian wall materials TE Savoldi, FB Scheufele, DA Drunkler, GJ da Silva, JD de Lima, ... Journal of Food Processing and Preservation, e16596 , 2022 2022 Citations: 8
Influence of different preparation techniques on the composition and antioxidant action of curcumin and curcuminoids. J Pelissari Marchi, FA dos Reis Lívero, A Assunção Soare, ... Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 21 (1) , 2022 2022 Citations: 4
Influencia de diferentes técnicas de preparación en la composición y acción antioxidante de la curcumina y los curcuminoides JP Marchi, FA dos Reis Lívero, AA Soare, GJ da Silva, AKV Soares, ... Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 21 … , 2022 2022 Citations: 1
Chemical and nutritional characterization of bean genotypes (Phaseolus vulgaris L.). J Destro de Lima, W Ribeiro Rivadavea, SA Frehner Kavalco, ... AIMS Agriculture & Food 6 (4) , 2021 2021 Citations: 1
Microbial enzymes and soil health GJ da Silva, WR Rivadavea, JD de Lima, PHR Monteiro, FB da Silva Plant growth-promoting microbes for sustainable biotic and abiotic stress … , 2021 2021 Citations: 14
MOST CITED SCHOLAR PUBLICATIONS
Origin, Domestication, and Dispersing of Pear ( Pyrus spp.) GJ Silva, TM Souza, RL Barbieri, A Costa de Oliveira Advances in Agriculture 2014 (1), 541097 , 2014 2014 Citations: 171
A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum J King, S Grewal, C Yang, S Hubbart, D Scholefield, S Ashling, ... Plant Biotechnology Journal 15 (2), 217-226 , 2017 2017 Citations: 163
Genetic variability in Brazilian wheat cultivars assessed by microsatellite markers I Schuster, ESN Vieira, GJ Silva, FA Franco, VS Marchioro Genetics and Molecular Biology 32 (3), 557-563 , 2009 2009 Citations: 57
Lithium bioaccumulation in Lentinus crinitus mycelial biomass as a potential functional food MGI Faria, KV Avelino, JS do Valle, GJ da Silva, AC Gonçalves Jr, ... Chemosphere 235, 538-542 , 2019 2019 Citations: 39
Pear ( Pyrus spp.) Breeding GJ da Silva, F Villa, F Grimaldi, PS da Silva, JF Welter Advances in Plant Breeding Strategies: Fruits: Volume 3, 131-163 , 2018 2018 Citations: 22
Combinação de espaçamento entrelinhas e densidade populacional no aumento da produtividade em milho R BOIAGO, R GARCIA, AR SCHUELTER, R BARRETO, GJ DA SILVA, ... International Journal of Maize & Sorghum 16 (3), 440-448 , 2017 2017 Citations: 17
Expanding agricultural potential through biological nitrogen fixation: Recent advances and diversity of diazotrophic bacteria JD de Lima, AJ de Souza, ALP Nunes, WR Rivadavea, GC Zaro, ... Australian Journal of Crop Science 18 (6), 324-333 , 2024 2024 Citations: 15
Microbial enzymes and soil health GJ da Silva, WR Rivadavea, JD de Lima, PHR Monteiro, FB da Silva Plant growth-promoting microbes for sustainable biotic and abiotic stress … , 2021 2021 Citations: 14
Genes acting on transcriptional control during abiotic stress responses GJ da Silva, A Costa de Oliveira Advances in Agriculture 2014 (1), 587070 , 2014 2014 Citations: 14
Direct organogenesis protocol from shoot segments of'Solanum tuberosum'cv. Monalisa NA Campos, GJ da Silva, MFB de Paula, TB Rodrigues, LAZ Rodrigues, ... Australian Journal of Crop Science 10 (7), 964-968 , 2016 2016 Citations: 11
Potential of endophytic bacteria from Acacia mearnsii: Phosphate solubilization, indole acetic acid production, and application in wheat JD de Lima, PHR Monteiro, WR Rivadavea, M Barbosa, RD Cordeiro, ... Applied Soil Ecology 196, 105315 , 2024 2024 Citations: 10
Plant growth promoting rhizobacteria in amelioration of abiotic stresses: A functional interplay and prospective PHR Monteiro, FB da Silva, CM de Abreu, GJ da Silva Plant Growth-Promoting Microbes for Sustainable Biotic and Abiotic Stress … , 2021 2021 Citations: 10
Antioxidant and antimicrobial activity of Brunfelsia uniflora leaf extract. LCT Thiesen, IM Colla, GJ Silva, MG Kubiak, MGI Faria, ZC Gazim, ... 2018 Citations: 9
Microencapsulation of Saccharomyces boulardii using vegan and vegetarian wall materials TE Savoldi, FB Scheufele, DA Drunkler, GJ da Silva, JD de Lima, ... Journal of Food Processing and Preservation, e16596 , 2022 2022 Citations: 8
Therapeutic potential of Zingiberaceae in Alzheimer’s disease W de Campos Bortolucci, JR Trettel, DM Bernardi, MMQ Souza, AD Lopes, ... Boletín Latinoam. Y Caribe Plantas Med. Y Aromáticas 19, 428-465 , 2020 2020 Citations: 8
Produção de haplóides androgenéticos em milho GJ da Silva, CT Guimarães, SN Parentoni, M Rabel, UG de Paula Lana, ... 2009 Citations: 8
Chemical and nutritional characterization of bean genotypes (Phaseolus vulgaris L.) JD de Lima, WR Rivadavea, SAF Kavalco, AC Gonçalves, AD Lopes, ... AIMS Agriculture and Food 6 (4) , 2021 2021 Citations: 7
Functionally divergent splicing variants of the rice AGAMOUS ortholog OsMADS3 are evolutionary conserved in grasses L Dreni, A Ravasio, N Gonzalez-Schain, S Jacchia, GJ Da Silva, ... Frontiers in plant science 11, 637 , 2020 2020 Citations: 7
Advances in plant breeding strategies: fruits GJ Da Silva, F Villa, F Grimaldi, PS da Silva, JF Welter Springer International Publishing , 2018 2018 Citations: 7
Somatic embryogenesis and plant regeneration in Brazilian rice genotypes GJ da Silva, RS dos Santos, TM Souza, ND Machado, JA Peters, ... Australian Journal of Crop Science 9 (11), 1126-1130 , 2015 2015 Citations: 6
