paolo bergese

Scopus Publications

A reliable high-throughput OECT-based bioelectronic system enabling pan-specific vesicle quantification
Giulia Frusconi, Angelo Musicò, Andrea Zendrini, Zsolt M. Kovács-Vajna, Lucia Paolini, Paolo Bergese, Fabrizio Torricelli
Biosensors and Bioelectronics X, 2026
Next-generation extracellular vesicle (EV) technologies are transforming biomanufacturing and functional product development. Quantifying EVs based on their membrane protein content is crucial for ensuring consistent yield, composition, and functional performance in manufacturing pipelines. Existing methods measure EV size and concentration but provide no information on membrane protein presence, whereas immunoaffinity approaches depend on specific markers that may be inconsistently expressed due to EV heterogeneity. Here we demonstrate a reliable bioelectronic system for pan-specific, high-throughput total EV quantification, achieving 12 independent parallel analysis in less than 15 min, with false-positive and false-negative rates below 1%. The system features multiple channel-functionalized arrays of organic electrochemical transistors (OECTs) in a 3 × 4 matrix configuration. Click chemistry enables real-time detection of EVs with membrane proteins, with a limit of identification of 6 × 106 particles ml−1 and a dynamic range spanning three orders of magnitude. By integrating functionalized OECT arrays with 3D-printed fluidic devices into a low-cost, disposable cartridge operated by a compact silicon-based electronic reader, the system enables rapid, reliable, and pan-specific total EV quantification in a user-friendly format, opening opportunities for in-line quality control and scalable implementation in biomanufacturing and pharmaceutical processes.
Unraveling the Heterogeneity of Cargo Distribution in the Exogenous Association of Proteins With Extracellular Vesicles
Karl Normak, Marcell Papp, Carolina Paganini, Andrea Zendrini, Paolo Bergese, Dorothea Pinotsi, Paolo Arosio
Advanced Healthcare Materials, 2026
Extracellular vesicles (EVs) are emerging as promising nanocarriers for delivering molecules, including proteins. Various exogenous methods are proposed for loading EVs with specific cargo proteins. While the loading yield and the heterogeneity of cargo distribution are crucial quality attributes, a comprehensive quantification of these properties is still lacking. Here, we characterize the heterogeneity of EVs loaded with a model cargo protein, GFP, using various exogenous methods. A combination of biophysical methods is applied to quantify the overall yield and cargo distribution at both the ensemble and single‐particle levels. Among the loading methods evaluated, electroporation is most effective for associating GFP with EVs. However, the GFP molecules per vesicle is fewer than 100, representing approximately 4% of the maximum protein capacity that EVs can potentially accommodate. Across all loading methods, the distribution of protein content per vesicle displays significant heterogeneity and follows an exponential decay function, with a higher prevalence of vesicles featuring lower protein content and fewer with higher content. Moreover, loading efficiency increases with EV size. This study shows that overall yields of exogenous loading methods to associate proteins with EVs remain modest and the resulting distribution of cargo proteins associated with EVs is highly heterogeneous.
Ethanol-Guided Hybridization of Extracellular Vesicles with Liquid-Crystalline Lipid Nanoparticles
Valentina Pacciani, Jacopo Cardellini, Arianna Balestri, Marta Rojas-Rodríguez, Martino Calamai, Mattia Tiboni, Luca Casettari, Catherine E. Saunders, Anam A. Karimi, Gennaro Sanità, Emanuela Esposito, Andrea Zendrini, Annalisa Radeghieri, Lucia Paolini, Paolo Bergese, Costanza Montis, Lucrezia Caselli, Debora Berti
ACS Applied Materials and Interfaces, 2026
High Resolution Image Download MS PowerPoint Slide Hybrid nanosystems that integrate biological and synthetic lipid assemblies hold great promise for tailoring nanoscale interfaces with programmable chemical and structural functionality. However, existing approaches to hybridize extracellular vesicles (EVs) with lipid nanoparticles (LNPs) compromise either the EV bioactivity or the native supramolecular organization of synthetic LNPs, undermining structure-dependent functionality. Here, we introduce an ethanol-mediated microfluidic assembly route that enables the in situ formation and hybridization of nonlamellar liquid-crystalline lipid nanoparticles (LCNPs) with red-blood-cell-derived EVs (RBCEVs) in a single step. This process exploits ethanol-induced interfacial reorganization to drive EV incorporation without compromising the LCNP cubic architecture. Synchrotron small-angle X-ray scattering (SAXS) and cryogenic electron microscopy reveal hybrid nanoparticles that retain long-range cubic order, with RBCEV membrane proteins localized within phase-segregated nanodomains. Single-particle Raman analysis and enzymatic assays confirm molecular-level hybridization and preserved EV biofunctionality. Hybrid LCNPs also exhibit enhanced uptake in HEK293t cells. Mechanistic SAXS studies uncover that ethanol transiently stabilizes a swollen sponge-like intermediate, which mediates controlled fusion and acts as a structural template upon solvent removal, imparting long-lasting structural stability. This study elucidates the physicochemical mechanism of ethanol-guided hybridization between biogenic systems and soft nanostructured colloids, establishing design principles for structurally controlled nanohybrids with broad applicability in nanomedicine.
Orthogonal Investigation at Single-Particle and Ensemble Levels Uncovers Lipoprotein-Extracellular Vesicle Binding
Angelo Musicò, Roberto Frigerio, Karl Normak, Sabrina Scolari, Alessandro Gori, Paolo Arosio, Annalisa Radeghieri, Lucia Paolini, Miriam Romano, Irantzu Llarena, Sergio E. Moya, Andrea Zendrini, Paolo Bergese
Analytical Chemistry, 2026
Mesoscale interactions critically shape the biological identity of extracellular nanoparticles, including extracellular vesicles. These interactions encompass biomolecular coronas, transient aggregation, and fusion events. Among them, the interaction between extracellular vesicles and lipoproteins has recently garnered significant attention due to their potential impact on functionality and in vivo fate of extracellular vesicles. In this work, we present a first investigation of the binding between human red blood cell-derived extracellular vesicles and lipoproteins across multiple scales, in both buffer and plasma. Red blood cell-derived extracellular vesicles were selected as a model system for their physicochemical homogeneity, potential in personalized medicine, and production scalability. To achieve this, we employed an ad hoc suite of orthogonal analytical techniques: fluorescence cross-correlation spectroscopy (FCCS), super-resolution microscopy, flow cytometry, and Single Molecule Array assays (Simoa). Our results reveal class-specific and context-dependent extracellular vesicle-lipoprotein associations. Notably, lipoproteins bind to extracellular vesicles with affinities ranging from 10 nM to 1 μM and with up to 100% extracellular vesicles interacting with high-density lipoproteins in the presence of plasma proteins. These findings uncover a complex and dynamic interactome of red blood cell-derived extracellular vesicles across lipoprotein classes. This work establishes a robust methodological framework for studying mesoscale interactions of extracellular nanoparticles under physiologically relevant conditions. Its versatility allows for its application to diverse interaction scenarios, supporting systematic investigation of context-dependent effects on EV-LP binding.
Red blood cell-derived extracellular vesicles as biomaterials: the opportunity of freezing-induced accelerated aging
Lucia Paolini, Miriam Romano, Valentina Mangolini, Selene Tassoni, Shuhan Jiang, Elena Laura Mazzoldi, Angelo Musicò, Andrea Zendrini, Anna Kashkanova, Vahid Sandoghdar, Anna C. Berardi, Silvia Clara Giliani, Paolo Bergese, Annalisa Radeghieri
Biomaterials Science, 2026
Freeze–thaw-induced ageing enables the generation of homogeneous RBC-EV populations with preserved bioactivity, enhancing their standardization and applicability as biomaterials in nanomedicine.
Spotlight on innovation: key insights from the extracellular vesicle cluster meeting at the European Innovation Council
Giorgia Adamo, Ioannis Amarantos, Iordanis Arzimanoglou, Paolo Bergese, Antonella Bongiovanni, Benedetta Bussolati, Valentina Cauda, Marcella Chiari, George A. Garinis, Bernd Giebel, Paolo Guazzi, Marcin Jurga, Christian Neri, Pelle Ohlsson, Eva Rohde, Goreti Sales, Irene Trapani, Pieter Vader, Seppo Vainio, Marca H.M. Wauben, María Yáñez-Mó, Natasa Zarovni
Cytotherapy, 2026
The field of extracellular vesicles (EVs) is rapidly advancing, offering promising applications in diagnostics, therapeutics, and drug delivery. However, the translation of EV-based technologies to the clinic faces significant challenges related to heterogeneity, scalable biomanufacturing, and regulatory compliance. To address these issues, the European Innovation Council (EIC), in collaboration with the Horizon2020-funded BOW project, organized the "Extracellular Vesicle EIC Cluster Meeting," bringing together researchers, startups, and regulatory stakeholders across Europe. Discussions focused on overcoming bottlenecks in EV production, standardization, and clinical readiness. Key outcomes included the need for application-specific benchmarks, robust manufacturing pipelines, and regulatory frameworks tailored to EVs. The event emphasized the importance of interdisciplinary collaboration, coopetition, and continued EU funding to drive innovation and strengthen Europe's leadership in the EV field.
The gold nanoparticle-lipid membrane synergy for nanomedical applications
Lucrezia Caselli, Lucia Paolini, Wye-Khay Fong, Costanza Montis, Andrea Zendrini, Jacopo Cardellini, Paolo Bergese, Debora Berti
Nanoscale Horizons, 2025
The integration of gold nanoparticles (AuNPs) with lipid bilayers, gives rise to powerful synergistic effects arising from nanoscale interactions.
The extracellular vesicle biomolecular corona: current insights and diagnostic potential
Selene Tassoni, Paolo Bergese, Annalisa Radeghieri
Nanomedicine, 2025
Extracellular vesicles (or EVs) are biogenic nanoparticles released by all cell types, playing a central role in intercellular communication by transporting proteins, nucleic acids, and metabolites. Over the past 5 years, it has become evident that, similarly to synthetic nanoparticles, EVs acquire a dynamic layer of biomolecules from the surrounding biological fluids. These spatially correlated and time-resolved interactions between EVs and the other molecular and nanoscale components of the biological environment are named biomolecular corona, and concur in shaping the biological identity, function, and fate of EVs. In this review, we first outline the physicochemical interactions that drive biomolecular corona formation on EVs and its principal biomolecular components. We then compare ex situ and in situ characterization techniques currently employed to study the EVs and EV corona complexes, highlighting their respective advantages and limitations. Finally, we discuss how advancing our understanding of the EV corona could significantly improve the robustness of EV-based biomarkers and enhance the specificity of liquid biopsy strategies, ultimately contributing to the development of next-generation precision medicine and personalized diagnostics.
Nanoplasmonic Isosbestics Uncover Mesoscale Assembly of Gold Nanoparticles on Soft Templates
Jacopo Cardellini, Ilaria De Santis, Giuseppe Emanuele Lio, Marco Brucale, Francesco Valle, Virginia Catani, Ilenia Mastrolia, Marta Calabria, Massimo Dominici, Andrea Zendrini, Annalisa Radeghieri, Lucia Paolini, Paolo Bergese, Lucrezia Caselli, Debora Berti, Costanza Montis
Journal of the American Chemical Society, 2025
Assembly of plasmonic nanoparticles (NPs) generates unique optical properties through coupling of the localized surface plasmon resonance (LSPR) of individual NPs. However, precisely controlling and monitoring how mesoscale assembly dictates final optical properties remain key challenges in designing advanced plasmonic materials. Here, we introduce "nanoplasmonic isosbestics" as optical descriptors of the mesoscale organization of gold nanoparticles (AuNPs) on soft templates. Unlike isosbestic points in molecular spectroscopy, which describe chemical equilibria, our numerical simulations demonstrate that nanoplasmonic isosbestics emerge from the coexistence of individual AuNPs and AuNP clusters, where the interparticle spacing determines the isosbestic wavelength. By templating AuNP assembly onto synthetic free-standing lipid bilayers with tunable membrane rigidity, we experimentally achieve precise control over interparticle spacing and prove that it is mirrored by univocal modulation of the isosbestic wavelength. This provides a fundamental understanding of the structure-function relationship in plasmonic systems, linking, for the first time, nanoplasmonic isosbestics to interparticle spacing and equilibrium structure in plasmonic assemblies. On the analytical perspective, nanoplasmonic isosbestics provide noninvasive optical fingerprints of the templates, opening to appealing applications. As a proof of concept, we apply this approach to profile the stiffness of two extracellular vesicle (EVs) classes─mesenchymal stem cell (MSC)-derived and red blood cell-derived EVs─both recognized for their biological and translational potential.
MicroRNA-142-3p shuttling in extracellular vesicles marks regulatory T cell dysfunction in multiple sclerosis
Giusy De Rosa, Claudia Russo, Silvia Garavelli, Dario Di Silvestre, Ilaria Spatocco, Giorgia Mele, Claudia La Rocca, Alessandra Colamatteo, Fortunata Carbone, Clorinda Fusco, Fabiana Passaro, Donatella Carpi, Elena Tagliabue, Francesco Prattichizzo, Francesca Brambilla, Pierluigi Mauri, Mirjam Hoxha, Valentina Bollati, Ilaria Giusti, Vincenza Dolo, Paola D’Antona, Paola Campomenosi, Valentina Mangolini, Annalisa Radeghieri, Paolo Bergese, Ivana Morabito, Alessandra Mandelli, Annamaria Finardi, Francesca Beretta, Edoardo Dalmato Schilke, Guido Cavaletti, Ettore Dolcetti, Fabio Buttari, Gianmarco Abbadessa, Simona Bonavita, Giacomo Lus, Elisabetta Signoriello, Roberta Lanzillo, Vincenzo Brescia Morra, Maria Mottola, Bruno Zuccarelli, Antonio Uccelli, Marco Salvetti, Diego Centonze, Roberto Furlan, Giuseppe Matarese, Claudio Procaccini, Paola de Candia
Science Translational Medicine, 2025
CD4 + CD25 hi FoxP3 + regulatory T cells (T reg cells) are key controllers of immune self-tolerance, and their suppressive function is impaired in people with relapsing-remitting multiple sclerosis (pwRR-MS). Because the mechanisms underlying this condition are still ill-defined, we investigated the role of T reg cell–derived extracellular vesicles (T reg -EVs) in T reg cell dysfunction observed in pwRR-MS. We found that T reg -EVs from healthy individuals inhibit CD4 + conventional T (T conv ) cells by shuttling miR-142-3p from the T reg cell to the T conv cell. There, miR-142-3p down-regulated mRNAs necessary for T conv cell growth and effector functions, such as the redox controller cystine carrier SLC7A11 . However, T reg cells from pwRR-MS released EVs containing reduced amounts of miR-142-3p, resulting in impaired suppressive function. Furthermore, T reg -EV miR-142-3p inversely correlated with the disability score and gadolinium-enhancing lesions in pwRR-MS. Together, our results elucidate a molecular mechanism involving miR-142-3p shuttled by T reg -EVs in the control of immune self-tolerance and unveil its pathogenetic implications in human autoimmunity.
Microfluidics-Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle-Vesicle Hybrids
Jacopo Cardellini, Karl Normak, Michael Gerlt, Katarzyna Makasewicz, Charlotte Seiffert, Umberto Capasso Palmiero, Suiying Ye, Manuel A. González Gómez, Yolanda Piñero, José Rivas, Antonella Bongiovanni, Paolo Bergese, Paolo Arosio
Advanced Healthcare Materials, 2025
The physico-chemical landscape of extracellular vesicles
Mauro Manno, Antonella Bongiovanni, Leonid Margolis, Paolo Bergese, Paolo Arosio
Nature Reviews Bioengineering, 2025
Helminth extracellular vesicles co-opt host monocytes to drive T cell anergy
Anne Borup, Mohammad Farouq Sharifpour, Litten S. Rossen, Bradley Whitehead, Anders T. Boysen, Rikke Olesen, Anja B. Bohn, Andrea Ridolfi, Marco Brucale, Francesco Valle, Lucia Paolini, Annalisa Radeghieri, Paolo Bergese, Kim Miles, Margaret Veitch, Tamara Thomas, Roland Ruscher, Phurpa Wangchuk, Paul Giacomin, Alex Loukas, Peter Nejsum
Journal of Extracellular Vesicles, 2025
A decision-making tool for navigating extracellular vesicle research and product development
Francesca Loria, Sabrina Picciotto, Giorgia Adamo, Andrea Zendrini, Samuele Raccosta, Mauro Manno, Paolo Bergese, Giovanna L. Liguori, Antonella Bongiovanni, Nataša Zarovni
Journal of Extracellular Vesicles, 2024
Cell damage shifts the microRNA content of small extracellular vesicles into a Toll-like receptor 7-activating cargo capable to propagate inflammation and immunity
Valentina Salvi, Carolina Gaudenzi, Barbara Mariotti, Gaia Giongrandi, Silvia Alacqua, Veronica Gianello, Tiziana Schioppa, Laura Tiberio, Angela Ceribelli, Carlo Selmi, Paolo Bergese, Stefano Calza, Annalisa Del Prete, Silvano Sozzani, Flavia Bazzoni, Daniela Bosisio
Cell Communication and Signaling, 2024
Extracellular vesicles from the microalga Tetraselmis chuii are biocompatible and exhibit unique bone tropism along with antioxidant and anti-inflammatory properties
Giorgia Adamo, Pamela Santonicola, Sabrina Picciotto, Paola Gargano, Aldo Nicosia, Valeria Longo, Noemi Aloi, Daniele P. Romancino, Angela Paterna, Estella Rao, Samuele Raccosta, Rosina Noto, Monica Salamone, Irene Deidda, Salvatore Costa, Caterina Di Sano, Giuseppina Zampi, Svenja Morsbach, Katharina Landfester, Paolo Colombo, Mingxing Wei, Paolo Bergese, Nicolas Touzet, Mauro Manno, Elia Di Schiavi, Antonella Bongiovanni
Communications Biology, 2024
Extracellular vesicles of different cellular origin feature distinct biomolecular corona dynamics
Angelo Musicò, Andrea Zendrini, Santiago Gimenez Reyes, Valentina Mangolini, Lucia Paolini, Miriam Romano, Andrea Papait, Antonietta Rosa Silini, Paolo Di Gianvincenzo, Arabella Neva, Marina Cretich, Ornella Parolini, Camillo Almici, Sergio E. Moya, Annalisa Radeghieri, Paolo Bergese
Nanoscale Horizons, 2024
Advances in Extracellular Vesicle Research Over the Past Decade: Source and Isolation Method are Connected with Cargo and Function
Rodolphe Poupardin, Martin Wolf, Nicole Maeding, Liliia Paniushkina, Sven Geissler, Paolo Bergese, Kenneth W. Witwer, Katharina Schallmoser, Gregor Fuhrmann, Dirk Strunk
Advanced Healthcare Materials, 2024
Characterization of polyethylene terephthalate (PET) and polyamide (PA) true-to-life nanoplastics and their biological interactions
Serena Ducoli, Stefania Federici, Mariacristina Cocca, Gennaro Gentile, Andrea Zendrini, Paolo Bergese, Laura E. Depero
Environmental Pollution, 2024
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrügger, Juan M. Falcon‐Perez, Qing‐Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic‐Talisman, Nicole A. Kruh‐Garcia, Vroniqa Ku'ulei‐Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens‐Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter‐Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J. C. van Herwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, Clotilde Théry, Kenneth W. Witwer, and
Journal of Extracellular Vesicles, 2024
International Society for Extracellular Vesicles workshop. QuantitatEVs: Multiscale analyses, from bulk to single extracellular vesicle
Manuela Basso, Alessandro Gori, Caterina Nardella, Mari Palviainen, Marija Holcar, Ioannis Sotiropoulos, Sylwia Bobis‐Wozowicz, Vito G. D'Agostino, Elena Casarotto, Yari Ciani, Shiro Suetsugu, Alice Gualerzi, Lorena Martin‐Jaular, Daniela Boselli, Anna Kashkanova, Pietro Parisse, Lien Lippens, Martina Pagliuca, Martin Blessing, Roberto Frigerio, Thibaut Fourniols, Ana Meliciano, Anna Fietta, Paolo Vincenzo Fioretti, Karolina Soroczyńska, Silvia Picciolini, Amanda Salviano‐Silva, Paolo Bergese, Davide Zocco, Marcella Chiari, Guido Jenster, Levi Waldron, Aleksandar Milosavljevic, John Nolan, Marco P. Monopoli, Kenneth W. Witwer, Benedetta Bussolati, Dolores Di Vizio, Juan Falcon Perez, Metka Lenassi, Marina Cretich, Francesca Demichelis
Journal of Extracellular Biology, 2024
On the interaction and nanoplasmonics of gold nanoparticles and lipoproteins
Andrea Zendrini, Jacopo Cardellini, Roberto Frigerio, Marianna Bertoni, Debora Berti, Paolo Bergese
Jcis Open, 2023
Particle profiling of EV-lipoprotein mixtures by AFM nanomechanical imaging
Andrea Ridolfi, Laura Conti, Marco Brucale, Roberto Frigerio, Jacopo Cardellini, Angelo Musicò, Miriam Romano, Andrea Zendrini, Laura Polito, Greta Bergamaschi, Alessandro Gori, Costanza Montis, Stefano Panella, Lucio Barile, Debora Berti, Annalisa Radeghieri, Paolo Bergese, Marina Cretich, Francesco Valle
Journal of Extracellular Vesicles, 2023
Multiparametric Orthogonal Characterization of Extracellular Vesicles by Liquid Chromatography Combined with In-Line Light Scattering and Fluorescence Detection
Karl Normak, Marcell Papp, Michael Ullmann, Carolina Paganini, Mauro Manno, Antonella Bongiovanni, Paolo Bergese, Paolo Arosio
Analytical Chemistry, 2023
Surface functionalization of extracellular vesicle nanoparticles with antibodies: a first study on the protein corona “variable”
Angelo Musicò, Rossella Zenatelli, Miriam Romano, Andrea Zendrini, Silvia Alacqua, Selene Tassoni, Lucia Paolini, Chiara Urbinati, Marco Rusnati, Paolo Bergese, Giuseppe Pomarico, Annalisa Radeghieri
Nanoscale Advances, 2023
Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics
Jacopo Cardellini, Andrea Ridolfi, Melissa Donati, Valentina Giampietro, Mirko Severi, Marco Brucale, Francesco Valle, Paolo Bergese, Costanza Montis, Lucrezia Caselli, Debora Berti
Journal of Colloid and Interface Science, 2023
Synthesis and Characterization of a Biocompatible Nanoplatform Based on Silica-Embedded SPIONs Functionalized with Polydopamine
Miriam Romano, Manuel Antonio González Gómez, Pamela Santonicola, Noemi Aloi, Svenja Offer, Jana Pantzke, Samuele Raccosta, Valeria Longo, Alessandro Surpi, Silvia Alacqua, Giuseppina Zampi, Valentin Alek Dediu, Bernhard Michalke, Ralf Zimmerman, Mauro Manno, Yolanda Piñeiro, Paolo Colombo, Elia Di Schiavi, José Rivas, Paolo Bergese, Sebastiano Di Bucchianico
ACS Biomaterials Science and Engineering, 2023
The biomolecular corona of extracellular nanoparticles holds new promises for advancing clinical molecular diagnostics
Annalisa Radeghieri, Paolo Bergese
Expert Review of Molecular Diagnostics, 2023
On the surface-to-bulk partition of proteins in extracellular vesicles
Andrea Zendrini, Giorgia Guerra, Krizia Sagini, Tatyana Vagner, Dolores Di Vizio, Paolo Bergese
Colloids and Surfaces B Biointerfaces, 2022
Large-scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop
Lucia Paolini, Marta Monguió‐Tortajada, Marta Costa, Fabio Antenucci, Mario Barilani, Marta Clos‐Sansalvador, André Cronemberger Andrade, Tom A. P. Driedonks, Sara Giancaterino, Stephanie M. Kronstadt, Rachel R. Mizenko, Muhammad Nawaz, Xabier Osteikoetxea, Carla Pereira, Surya Shrivastava, Anders Toftegaard Boysen, Simonides Immanuel van de Wakker, Martijn J. C. van Herwijnen, Xiaoqin Wang, Dionysios C. Watson, Mario Gimona, Maria Kaparakis‐Liaskos, Konstantin Konstantinov, Sai Kiang Lim, Nicole Meisner‐Kober, Michiel Stork, Peter Nejsum, Annalisa Radeghieri, Eva Rohde, Nicolas Touzet, Marca H. M. Wauben, Kenneth W. Witwer, Antonella Bongiovanni, Paolo Bergese
Journal of Extracellular Biology, 2022
Active antithrombin glycoforms are selectively physiosorbed on plasma extracellular vesicles
Annalisa Radeghieri, Silvia Alacqua, Andrea Zendrini, Vanessa Previcini, Francesca Todaro, Giuliana Martini, Doris Ricotta, Paolo Bergese
Journal of Extracellular Biology, 2022
Human Microglia Extracellular Vesicles Derived from Different Microglia Cell Lines: Similarities and Differences
Lorenzo Ceccarelli, Laura Marchetti, Milena Rizzo, Aldo Moscardini, Valentina Cappello, Eleonora Da Pozzo, Miriam Romano, Chiara Giacomelli, Paolo Bergese, Claudia Martini
ACS Omega, 2022
Comparison of separation methods for immunomodulatory extracellular vesicles from helminths
Anne Borup, Anders T. Boysen, Andrea Ridolfi, Marco Brucale, Francesco Valle, Lucia Paolini, Paolo Bergese, Peter Nejsum
Journal of Extracellular Biology, 2022
A different protein corona cloaks “true-to-life” nanoplastics with respect to synthetic polystyrene nanobeads
Serena Ducoli, Stefania Federici, Roland Nicsanu, Andrea Zendrini, Claudio Marchesi, Lucia Paolini, Annalisa Radeghieri, Paolo Bergese, Laura E. Depero
Environmental Science Nano, 2022
A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles
Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Nanoscale Horizons, 2021
Corrigendum: Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions (Frontiers in Bioengineering and Biotechnology, (2020), 7, (452), 10.3389/fbioe.2019.00452)
Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
Frontiers in Bioengineering and Biotechnology, 2021
Nanoanalytical analysis of bisphosphonate-driven alterations of microcalcifications using a 3D hydrogel system and in vivo mouse model
Jessica L. Ruiz, Joshua D. Hutcheson, Luis Cardoso, Amirala Bakhshian Nik, Alexandra Condado de Abreu, Tan Pham, Fabrizio Buffolo, Sara Busatto, Stefania Federici, Andrea Ridolfi, Masanori Aikawa, Sergio Bertazzo, Paolo Bergese, Sheldon Weinbaum, Elena Aikawa
Proceedings of the National Academy of Sciences of the United States of America, 2021
Biogenic, hybrid and synthetic vesicles
Paolo Bergese, Kimberly Hamad-Schifferli, Costanza Montis
Biochimica Et Biophysica Acta General Subjects, 2021
BMP6 binding to heparin and heparan sulfate is mediated by N-terminal and C-terminal clustered basic residues
Andrea Denardo, Stefano Elli, Stefania Federici, Michela Asperti, Magdalena Gryzik, Paola Ruzzenenti, Fernando Carmona, Paolo Bergese, Annamaria Naggi, Paolo Arosio, Maura Poli
Biochimica Et Biophysica Acta General Subjects, 2021
Thermodynamics of (nano)interfaces
Paolo Bergese, Italo Colombo, Stefania Federici
Colloidal Foundations of Nanoscience Second Edition, 2021
AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles
Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T. Boysen, Francesca Loria, Martijn J. C. van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H. M. Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Analytical Chemistry, 2020
Shedding light on membrane-templated clustering of gold nanoparticles
Costanza Montis, Lucrezia Caselli, Francesco Valle, Andrea Zendrini, Francesco Carlà, Ralf Schweins, Marco Maccarini, Paolo Bergese, Debora Berti
Journal of Colloid and Interface Science, 2020
Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces
Costanza Montis, Annalisa Salvatore, Francesco Valle, Lucia Paolini, Francesco Carlà, Paolo Bergese, Debora Berti
Journal of Colloid and Interface Science, 2020
Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions
Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
Frontiers in Bioengineering and Biotechnology, 2020
Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin
Lucia Paolini, Stefania Federici, Giovanni Consoli, Diletta Arceri, Annalisa Radeghieri, Ivano Alessandri, Paolo Bergese
Journal of Extracellular Vesicles, 2020
Extracellular vesicles in regenerative medicine
Miriam Romano, Andrea Zendrini, Lucia Paolini, Sara Busatto, Anna C. Berardi, Paolo Bergese, Annalisa Radeghieri
Nanomaterials for Theranostics and Tissue Engineering Techniques Trends and Applications, 2020
The nanostructured secretome
S. Busatto, A. Zendrini, A. Radeghieri, L. Paolini, M. Romano, M. Presta, P. Bergese
Biomaterials Science, 2020
Extracellular vesicles from rat-bone-marrow mesenchymal stromal/stem cells improve tendon repair in rat Achilles tendon injury model in dose-dependent manner: A pilot study
Clarissa Gissi, Annalisa Radeghieri, Cristina Antonetti Lamorgese Passeri, Marialucia Gallorini, Lucia Calciano, Francesco Oliva, Francesca Veronesi, Andrea Zendrini, Amelia Cataldi, Paolo Bergese, Nicola Maffulli, Anna Concetta Berardi
Plos One, 2020
Exploitation of a novel biosensor based on the full-length human F508del-CFTR with computational studies, biochemical and biological assays for the characterization of a new Lumacaftor/Tezacaftor analogue
Pasqualina D’Ursi, Matteo Uggeri, Chiara Urbinati, Enrico Millo, Giulia Paiardi, Luciano Milanesi, Robert C. Ford, Jack Clews, Xin Meng, Paolo Bergese, Andrea Ridolfi, Nicoletta Pedemonte, Paola Fossa, Alessandro Orro, Marco Rusnati
Sensors and Actuators B Chemical, 2019
Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop
Ashley E. Russell, Alexandra Sneider, Kenneth W. Witwer, Paolo Bergese, Suvendra N. Bhattacharyya, Alexander Cocks, Emanuele Cocucci, Uta Erdbrügger, Juan M. Falcon‐Perez, David W. Freeman, Thomas M. Gallagher, Shuaishuai Hu, Yiyao Huang, Steven M. Jay, Shin‐ichi Kano, Gregory Lavieu, Aleksandra Leszczynska, Alicia M. Llorente, Quan Lu, Vasiliki Mahairaki, Dillon C. Muth, Nicole Noren Hooten, Matias Ostrowski, Ilaria Prada, Susmita Sahoo, Tine Hiorth Schøyen, Lifu Sheng, Deanna Tesch, Guillaume Van Niel, Roosmarijn E. Vandenbroucke, Frederik J. Verweij, Ana V. Villar, Marca Wauben, Ann M. Wehman, Hang Yin, David Raul Francisco Carter, Pieter Vader
Journal of Extracellular Vesicles, 2019
Publisher Correction: On the issue of transparency and reproducibility in nanomedicine (Nature Nanotechnology, (2019), 14, 7, (629-635), 10.1038/s41565-019-0496-9)
Hon S. Leong, Kimberly S. Butler, C. Jeffrey Brinker, May Azzawi, Steve Conlan, Christine Dufès, Andrew Owen, Steve Rannard, Chris Scott, Chunying Chen, Marina A. Dobrovolskaia, Serguei V. Kozlov, Adriele Prina-Mello, Ruth Schmid, Peter Wick, Fanny Caputo, Patrick Boisseau, Rachael M. Crist, Scott E. McNeil, Bengt Fadeel, Lang Tran, Steffen Foss Hansen, Nanna B. Hartmann, Lauge P. W. Clausen, Lars M. Skjolding, Anders Baun, Marlene Ågerstrand, Zhen Gu, Dimitrios A. Lamprou, Clare Hoskins, Leaf Huang, Wantong Song, Huiliang Cao, Xuanyong Liu, Klaus D. Jandt, Wen Jiang, Betty Y. S. Kim, Korin E. Wheeler, Andrew J. Chetwynd, Iseult Lynch, Seyed Moein Moghimi, André Nel, Tian Xia, Paul S. Weiss, Bruno Sarmento, José das Neves, Hélder A. Santos, Luis Santos, Samir Mitragotri, Steve Little, Dan Peer, Mansoor M. Amiji, Maria José Alonso, Alke Petri-Fink, Sandor Balog, Aaron Lee, Barbara Drasler, Barbara Rothen-Rutishauser, Stefan Wilhelm, Handan Acar, Roger G. Harrison, Chuanbin Mao, Priyabrata Mukherjee, Rajagopal Ramesh, Lacey R. McNally, Sara Busatto, Joy Wolfram, Paolo Bergese, Mauro Ferrari, Ronnie H. Fang, Liangfang Zhang, Jie Zheng, Chuanqi Peng, Bujie Du, Mengxiao Yu, Danielle M. Charron, Gang Zheng, Chiara Pastore
Nature Nanotechnology, 2019
Publisher Correction: On the issue of transparency and reproducibility in nanomedicine (Nature Nanotechnology, (2019), 14, 7, (629-635), 10.1038/s41565-019-0496-9)
Hon S. Leong, Kimberly S. Butler, C. Jeffrey Brinker, May Azzawi, Steve Conlan, Christine Dufès, Andrew Owen, Steve Rannard, Chris Scott, Chunying Chen, Marina A. Dobrovolskaia, Serguei V. Kozlov, Adriele Prina-Mello, Ruth Schmid, Peter Wick, Fanny Caputo, Patrick Boisseau, Rachael M. Crist, Scott E. McNeil, Bengt Fadeel, Lang Tran, Steffen Foss Hansen, Nanna B. Hartmann, Lauge P. W. Clausen, Lars M. Skjolding, Anders Baun, Marlene Ågerstrand, Zhen Gu, Dimitrios A. Lamprou, Clare Hoskins, Leaf Huang, Wantong Song, Huiliang Cao, Xuanyong Liu, Klaus D. Jandt, Wen Jiang, Betty Y. S. Kim, Korin E. Wheeler, Andrew J. Chetwynd, Iseult Lynch, Sayed Moein Moghimi, André Nel, Tian Xia, Paul S. Weiss, Bruno Sarmento, José das Neves, Hélder A. Santos, Luis Santos, Samir Mitragotri, Steve Little, Dan Peer, Mansoor M. Amiji, Maria José Alonso, Alke Petri-Fink, Sandor Balog, Aaron Lee, Barbara Drasler, Barbara Rothen-Rutishauser, Stefan Wilhelm, Handan Acar, Roger G. Harrison, Chuanbin Mao, Priyabrata Mukherjee, Rajagopal Ramesh, Lacey R. McNally, Sara Busatto, Joy Wolfram, Paolo Bergese, Mauro Ferrari, Ronnie H. Fang, Liangfang Zhang, Jie Zheng, Chuanqi Peng, Bujie Du, Mengxiao Yu, Danielle M. Charron, Gang Zheng, Chiara Pastore
Nature Nanotechnology, 2019
On the issue of transparency and reproducibility in nanomedicine
Hon S. Leong, Kimberly S. Butler, C. Jeffrey Brinker, May Azzawi, Steve Conlan, Christine Dufés, Andrew Owen, Steve Rannard, Chris Scott, Chunying Chen, Marina A. Dobrovolskaia, Serguei V. Kozlov, Adriele Prina-Mello, Ruth Schmid, Peter Wick, Fanny Caputo, Patrick Boisseau, Rachael M. Crist, Scott E. McNeil, Bengt Fadeel, Lang Tran, Steffen Foss Hansen, Nanna B. Hartmann, Lauge P. W. Clausen, Lars M. Skjolding, Anders Baun, Marlene Ågerstrand, Zhen Gu, Dimitrios A. Lamprou, Clare Hoskins, Leaf Huang, Wantong Song, Huiliang Cao, Xuanyong Liu, Klaus D. Jandt, Wen Jiang, Betty Y. S. Kim, Korin E. Wheeler, Andrew J. Chetwynd, Iseult Lynch, Seyed Moein Moghimi, André Nel, Tian Xia, Paul S. Weiss, Bruno Sarmento, José das Neves, Hélder A. Santos, Luis Santos, Samir Mitragotri, Steve Little, Dan Peer, Mansoor M. Amiji, Maria José Alonso, Alke Petri-Fink, Sandor Balog, Aaron Lee, Barbara Drasler, Barbara Rothen-Rutishauser, Stefan Wilhelm, Handan Acar, Roger G. Harrison, Chuanbin Mao, Priyabrata Mukherjee, Rajagopal Ramesh, Lacey R. McNally, Sara Busatto, Joy Wolfram, Paolo Bergese, Mauro Ferrari, Ronnie H. Fang, Liangfang Zhang, Jie Zheng, Chuanqi Peng, Bujie Du, Mengxiao Yu, Danielle M. Charron, Gang Zheng, Chiara Pastore
Nature Nanotechnology, 2019
Analysis of a nanoparticle‑enriched fraction of plasma reveals miRNA candidates for down syndrome pathogenesis
Alessandro Salvi, Marika Vezzoli, Sara Busatto, Lucia Paolini, Teresa Faranda, Edoardo Abeni, Maria Caracausi, Francesca Antonaros, Allison Piovesan, Chiara Locatelli, Guido Cocchi, Gualtiero Alvisi, Giuseppina De Petro, Doris Ricotta, Paolo Bergese, Annalisa Radeghieri
International Journal of Molecular Medicine, 2019
Erratum: Analysis of a nanoparticle‑enriched fraction of plasma reveals miRNA candidates for down syndrome pathogenesis(Int J Mol Med (2019) 43(2303‑2318) DOI: 10.3892/ijmm.2019.4158)
Alessandro Salvi, Marika Vezzoli, Sara Busatto, Lucia Paolini, Teresa Faranda, Edoardo Abeni, Maria Caracausi, Francesca Antonaros, Allison Piovesan, Chiara Locatelli, Guido Cocchi, Gualtiero Alvisi, Giuseppina De Petro, Doris Ricotta, Paolo Bergese, Annalisa Radeghieri
International Journal of Molecular Medicine, 2019
Collapse of the plasmacytoid dendritic cell compartment in advanced cutaneous melanomas by components of the tumor cell secretome
Raffaella Vescovi, Matilde Monti, Daniele Moratto, Lucia Paolini, Francesca Consoli, Luisa Benerini, Laura Melocchi, Stefano Calza, Mariella Chiudinelli, Giulio Rossi, Mattia Bugatti, Michele Maio, Ester Fonsatti, Camillo Farisoglio, Michele Simbolo, Camillo Almici, Rosanna Verardi, Aldo Scarpa, Paolo Bergese, Ausilia Manganoni, Fabio Facchetti, William Vermi
Cancer Immunology Research, 2019
Uptake Profiles of Human Serum Exosomes by Murine and Human Tumor Cells through Combined Use of Colloidal Nanoplasmonics and Flow Cytofluorimetric Analysis
Sara Busatto, Arianna Giacomini, Costanza Montis, Roberto Ronca, Paolo Bergese
Analytical Chemistry, 2018
Exosome-delivered microRNAs promote IFN-α secretion by human plasmacytoid DCs via TLR7
Valentina Salvi, Veronica Gianello, Sara Busatto, Paolo Bergese, Laura Andreoli, Ugo D’Oro, Alessandra Zingoni, Angela Tincani, Silvano Sozzani, Daniela Bosisio
Jci Insight, 2018
Model lipid bilayers mimic non-specific interactions of gold nanoparticles with macrophage plasma membranes
Costanza Montis, Viola Generini, Giulia Boccalini, Paolo Bergese, Daniele Bani, Debora Berti
Journal of Colloid and Interface Science, 2018
Biogenic Supported Lipid Bilayers from Nanosized Extracellular Vesicles
Costanza Montis, Sara Busatto, Francesco Valle, Andrea Zendrini, Annalisa Salvatore, Yuri Gerelli, Debora Berti, Paolo Bergese
Advanced Biosystems, 2018
Interaction of extracellular vesicles with Si surface studied by nanomechanical microcantilever sensors
Stefania Federici, Andrea Ridolfi, Andrea Zendrini, Annalisa Radeghieri, Elza Bontempi, Laura Depero, Paolo Bergese
Applied Sciences Switzerland, 2018
Tangential flow filtration for highly efficient concentration of extracellular vesicles from large volumes of fluid
Sara Busatto, George Vilanilam, Taylor Ticer, Wen-Lang Lin, Dennis W. Dickson, Shane Shapiro, Paolo Bergese, Joy Wolfram
Cells, 2018
Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe
Marco P. Monopoli, Andrea Zendrini, Dan Wu, Shane Cheung, Gonzalo Sampedro, Brendan Ffrench, John Nolan, Olga Piskareva, Raymond L. Stalings, Serena Ducoli, Paolo Bergese, Donal F. O’Shea
Chemical Communications, 2018
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Clotilde Théry, Kenneth W Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon D Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, Tanina Arab, Fabienne Archer, Georgia K Atkin‐Smith, D Craig Ayre, Jean‐Marie Bach, Daniel Bachurski, Hossein Baharvand, Leonora Balaj, Shawn Baldacchino, Natalie N Bauer, Amy A Baxter, Mary Bebawy, Carla Beckham, Apolonija Bedina Zavec, Abderrahim Benmoussa, Anna C Berardi, Paolo Bergese, Ewa Bielska, Cherie Blenkiron, Sylwia Bobis‐Wozowicz, Eric Boilard, Wilfrid Boireau, Antonella Bongiovanni, Francesc E Borràs, Steffi Bosch, Chantal M Boulanger, Xandra Breakefield, Andrew M Breglio, Meadhbh Á Brennan, David R Brigstock, Alain Brisson, Marike LD Broekman, Jacqueline F Bromberg, Paulina Bryl‐Górecka, Shilpa Buch, Amy H Buck, Dylan Burger, Sara Busatto, Dominik Buschmann, Benedetta Bussolati, Edit I Buzás, James Bryan Byrd, Giovanni Camussi, David RF Carter, Sarah Caruso, Lawrence W Chamley, Yu‐Ting Chang, Chihchen Chen, Shuai Chen, Lesley Cheng, Andrew R Chin, Aled Clayton, Stefano P Clerici, Alex Cocks, Emanuele Cocucci, Robert J Coffey, Anabela Cordeiro‐da‐Silva, Yvonne Couch, Frank AW Coumans, Beth Coyle, Rossella Crescitelli, Miria Ferreira Criado, Crislyn D'Souza‐Schorey, Saumya Das, Amrita Datta Chaudhuri, Paola de Candia, Eliezer F De Santana, Olivier De Wever, Hernando A del Portillo, Tanguy Demaret, Sarah Deville, Andrew Devitt, Bert Dhondt, Dolores Di Vizio, Lothar C Dieterich, Vincenza Dolo, Ana Paula Dominguez Rubio, Massimo Dominici, Mauricio R Dourado, Tom AP Driedonks, Filipe V Duarte, Heather M Duncan, Ramon M Eichenberger, Karin Ekström, Samir EL Andaloussi, Celine Elie‐Caille, Uta Erdbrügger, Juan M Falcón‐Pérez, Farah Fatima, Jason E Fish, Miguel Flores‐Bellver, András Försönits, Annie Frelet‐Barrand, Fabia Fricke, Gregor Fuhrmann, Susanne Gabrielsson, Ana Gámez‐Valero, Chris Gardiner, Kathrin Gärtner, Raphael Gaudin, Yong Song Gho, Bernd Giebel, Caroline Gilbert, Mario Gimona, Ilaria Giusti, Deborah CI Goberdhan, André Görgens, Sharon M Gorski, David W Greening, Julia Christina Gross, Alice Gualerzi, Gopal N Gupta, Dakota Gustafson, Aase Handberg, Reka A Haraszti, Paul Harrison, Hargita Hegyesi, An Hendrix, Andrew F Hill, Fred H Hochberg, Karl F Hoffmann, Beth Holder, Harry Holthofer, Baharak Hosseinkhani, Guoku Hu, Yiyao Huang, Veronica Huber, Stuart Hunt, Ahmed Gamal‐Eldin Ibrahim, Tsuneya Ikezu, Jameel M Inal, Mustafa Isin, Alena Ivanova, Hannah K Jackson, Soren Jacobsen, Steven M Jay, Muthuvel Jayachandran, Guido Jenster, Lanzhou Jiang, Suzanne M Johnson, Jennifer C Jones, Ambrose Jong, Tijana Jovanovic‐Talisman, Stephanie Jung, Raghu Kalluri, Shin‐ichi Kano, Sukhbir Kaur, Yumi Kawamura, Evan T Keller, Delaram Khamari, Elena Khomyakova, Anastasia Khvorova, Peter Kierulf, Kwang Pyo Kim, Thomas Kislinger, Mikael Klingeborn, David J Klinke, Miroslaw Kornek, Maja M Kosanović, Árpád Ferenc Kovács, Eva‐Maria Krämer‐Albers, Susanne Krasemann, Mirja Krause, Igor V Kurochkin, Gina D Kusuma, Sören Kuypers, Saara Laitinen, Scott M Langevin, Lucia R Languino, Joanne Lannigan, Cecilia Lässer, Louise C Laurent, Gregory Lavieu, Elisa Lázaro‐Ibáñez, Soazig Le Lay, Myung‐Shin Lee, Yi Xin Fiona Lee, Debora S Lemos, Metka Lenassi, Aleksandra Leszczynska, Isaac TS Li, Ke Liao, Sten F Libregts, Erzsebet Ligeti, Rebecca Lim, Sai Kiang Lim, Aija Linē, Karen Linnemannstöns, Alicia Llorente, Catherine A Lombard, Magdalena J Lorenowicz, Ákos M Lörincz, Jan Lötvall, Jason Lovett, Michelle C Lowry, Xavier Loyer, Quan Lu, Barbara Lukomska, Taral R Lunavat, Sybren LN Maas, Harmeet Malhi, Antonio Marcilla, Jacopo Mariani, Javier Mariscal, Elena S Martens‐Uzunova, Lorena Martin‐Jaular, M Carmen Martinez, Vilma Regina Martins, Mathilde Mathieu, Suresh Mathivanan, Marco Maugeri, Lynda K McGinnis, Mark J McVey, David G Meckes, Katie L Meehan, Inge Mertens, Valentina R Minciacchi, Andreas Möller, Malene Møller Jørgensen, Aizea Morales‐Kastresana, Jess Morhayim, François Mullier, Maurizio Muraca, Luca Musante, Veronika Mussack, Dillon C Muth, Kathryn H Myburgh, Tanbir Najrana, Muhammad Nawaz, Irina Nazarenko, Peter Nejsum, Christian Neri, Tommaso Neri, Rienk Nieuwland, Leonardo Nimrichter, John P Nolan, Esther NM Nolte‐'t Hoen, Nicole Noren Hooten, Lorraine O'Driscoll, Tina O'Grady, Ana O'Loghlen, Takahiro Ochiya, Martin Olivier, Alberto Ortiz, Luis A Ortiz, Xabier Osteikoetxea, Ole Østergaard, Matias Ostrowski, Jaesung Park, D. Michiel Pegtel, Hector Peinado, Francesca Perut, Michael W Pfaffl, Donald G Phinney, Bartijn CH Pieters, Ryan C Pink, David S Pisetsky, Elke Pogge von Strandmann, Iva Polakovicova, Ivan KH Poon, Bonita H Powell, Ilaria Prada, Lynn Pulliam, Peter Quesenberry, Annalisa Radeghieri, Robert L Raffai, Stefania Raimondo, Janusz Rak, Marcel I Ramirez, Graça Raposo, Morsi S Rayyan, Neta Regev‐Rudzki, Franz L Ricklefs, Paul D Robbins, David D Roberts, Silvia C Rodrigues, Eva Rohde, Sophie Rome, Kasper MA Rouschop, Aurelia Rughetti, Ashley E Russell, Paula Saá, Susmita Sahoo, Edison Salas‐Huenuleo, Catherine Sánchez, Julie A Saugstad, Meike J Saul, Raymond M Schiffelers, Raphael Schneider, Tine Hiorth Schøyen, Aaron Scott, Eriomina Shahaj, Shivani Sharma, Olga Shatnyeva, Faezeh Shekari, Ganesh Vilas Shelke, Ashok K Shetty, Kiyotaka Shiba, Pia R‐M Siljander, Andreia M Silva, Agata Skowronek, Orman L Snyder, Rodrigo Pedro Soares, Barbara W Sódar, Carolina Soekmadji, Javier Sotillo, Philip D Stahl, Willem Stoorvogel, Shannon L Stott, Erwin F Strasser, Simon Swift, Hidetoshi Tahara, Muneesh Tewari, Kate Timms, Swasti Tiwari, Rochelle Tixeira, Mercedes Tkach, Wei Seong Toh, Richard Tomasini, Ana Claudia Torrecilhas, Juan Pablo Tosar, Vasilis Toxavidis, Lorena Urbanelli, Pieter Vader, Bas WM van Balkom, Susanne G van der Grein, Jan Van Deun, Martijn JC van Herwijnen, Kendall Van Keuren‐Jensen, Guillaume van Niel, Martin E van Royen, Andre J van Wijnen, M Helena Vasconcelos, Ivan J Vechetti, Tiago D Veit, Laura J Vella, Émilie Velot, Frederik J Verweij, Beate Vestad, Jose L Viñas, Tamás Visnovitz, Krisztina V Vukman, Jessica Wahlgren, Dionysios C Watson, Marca HM Wauben, Alissa Weaver, Jason P Webber, Viktoria Weber, Ann M Wehman, Daniel J Weiss, Joshua A Welsh, Sebastian Wendt, Asa M Wheelock, Zoltán Wiener, Leonie Witte, Joy Wolfram, Angeliki Xagorari, Patricia Xander, Jing Xu, Xiaomei Yan, María Yáñez‐Mó, Hang Yin, Yuana Yuana, Valentina Zappulli, Jana Zarubova, Vytautas Žėkas, Jian‐ye Zhang, Zezhou Zhao, Lei Zheng, Alexander R Zheutlin, Antje M Zickler, Pascale Zimmermann, Angela M Zivkovic, Davide Zocco, Ewa K Zuba‐Surma
Journal of Extracellular Vesicles, 2018
Exosomes secreted by hela cells shuttle on their surface the plasma membrane-associated sialidase NEU3
Lucia Paolini, Flavia Orizio, Sara Busatto, Annalisa Radeghieri, Roberto Bresciani, Paolo Bergese, Eugenio Monti
Biochemistry, 2017
Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication
Rodrigo P. Soares, Patrícia Xander, Adriana Oliveira Costa, Antonio Marcilla, Armando Menezes‐Neto, Hernando Del Portillo, Kenneth Witwer, Marca Wauben, Esther Nolte‐'T Hoen, Martin Olivier, Miriã Ferreira Criado, Luis Lamberti P. da Silva, Munira Muhammad Abdel Baqui, Sergio Schenkman, Walter Colli, Maria Julia Manso Alves, Karen Spadari Ferreira, Rosana Puccia, Peter Nejsum, Kristian Riesbeck, Allan Stensballe, Eline Palm Hansen, Lorena Martin Jaular, Reidun Øvstebø, Laura de la Canal, Paolo Bergese, Vera Pereira‐Chioccola, Michael W. Pfaffl, Joëlle Fritz, Yong Song Gho, Ana Claudia Torrecilhas
Journal of Extracellular Vesicles, 2017
Size distribution of extracellular vesicles by optical correlation techniques
Costanza Montis, Andrea Zendrini, Francesco Valle, Sara Busatto, Lucia Paolini, Annalisa Radeghieri, Annalisa Salvatore, Debora Berti, Paolo Bergese
Colloids and Surfaces B Biointerfaces, 2017
Probing lysine mono-methylation in histone H3 tail peptides with an abiotic receptor coupled to a non-plasmonic resonator
N. Bontempi, E. Biavardi, D. Bordiga, G. Candiani, I. Alessandri, P. Bergese, E. Dalcanale
Nanoscale, 2017
Cultured human amniocytes express hTERT, which is distributed between nucleus and cytoplasm and is secreted in extracellular vesicles
Annalisa Radeghieri, Giulia Savio, Andrea Zendrini, Giuseppe Di Noto, Alessandro Salvi, Paolo Bergese, Giovanna Piovani
Biochemical and Biophysical Research Communications, 2017
Embodied energy as key parameter for sustainable materials selection: The case of reusing coal fly ash for removing anionic surfactants
Alessandra Zanoletti, Stefania Federici, Laura Borgese, Paolo Bergese, Matteo Ferroni, Laura Eleonora Depero, Elza Bontempi
Journal of Cleaner Production, 2017
RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines
Martina Berardocco, Annalisa Radeghieri, Sara Busatto, Marialucia Gallorini, Chiara Raggi, Clarissa Gissi, Igea D’Agnano, Paolo Bergese, Armando Felsani, Anna C. Berardi
Oncotarget, 2017
Energetics of surface confined ferritin during iron loading
Stefania Federici, Francesco Padovani, Maura Poli, Fernando Carmona Rodriguez, Paolo Arosio, Laura E. Depero, Paolo Bergese
Colloids and Surfaces B Biointerfaces, 2016
Cavitands Endow All-Dielectric Beads with Selectivity for Plasmon-Free Enhanced Raman Detection of Nϵ-Methylated Lysine
Ivano Alessandri, Elisa Biavardi, Alessandra Gianoncelli, Paolo Bergese, Enrico Dalcanale
ACS Applied Materials and Interfaces, 2016
Residual matrix from different separation techniques impacts exosome biological activity
Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta, Paolo Bergese
Scientific Reports, 2016
Merging colloidal nanoplasmonics and surface plasmon resonance spectroscopy for enhanced profiling of multiple myeloma-derived exosomes
Giuseppe Di Noto, Antonella Bugatti, Andrea Zendrini, Elena Laura Mazzoldi, Alessandro Montanelli, Luigi Caimi, Marco Rusnati, Doris Ricotta, Paolo Bergese
Biosensors and Bioelectronics, 2016
Comparison between rice husk ash grown in different regions for stabilizing fly ash from a solid waste incinerator
L. Benassi, A. Bosio, R. Dalipi, L. Borgese, N. Rodella, M. Pasquali, L.E. Depero, P. Bergese, E. Bontempi
Journal of Environmental Management, 2015
Colorimetric nanoplasmonic assay to determine purity and titrate extracellular vesicles
Daniele Maiolo, Lucia Paolini, Giuseppe Di Noto, Andrea Zendrini, Debora Berti, Paolo Bergese, Doris Ricotta
Analytical Chemistry, 2015
Surfactant titration of nanoparticle-protein corona
Daniele Maiolo, Paolo Bergese, Eugene Mahon, Kenneth A. Dawson, Marco P. Monopoli
Analytical Chemistry, 2014
Cavitand-grafted silicon microcantilevers as a universal probe for illicit and designer drugs in water
Elisa Biavardi, Stefania Federici, Cristina Tudisco, Daniela Menozzi, Chiara Massera, Andrea Sottini, Guglielmo G. Condorelli, Paolo Bergese, Enrico Dalcanale
Angewandte Chemie International Edition, 2014
Interaction of nanoparticles with lipid membranes: A multiscale perspective
Costanza Montis, Daniele Maiolo, Ivano Alessandri, Paolo Bergese, Debora Berti
Nanoscale, 2014
Thermodynamics of (Nano)interfaces
Paolo Bergese, Italo Colombo
Colloidal Foundations of Nanoscience, 2014
Preface
Methods in Molecular Biology, 2013
Nanomechanics of surface DNA switches probed by captive contact angle
Daniele Maiolo, Stefania Federici, Lorenzo Ravelli, Laura E. Depero, Kimberly Hamad-Schifferli, Paolo Bergese
Journal of Colloid and Interface Science, 2013
Sensitive determination of the Young's modulus of thin films by polymeric microcantilevers
Paolo Colombi, Paolo Bergese, Elza Bontempi, Laura Borgese, Stefania Federici, Stephan Sylvest Keller, Anja Boisen, Laura Eleonora Depero
Measurement Science and Technology, 2013
Leveraging on nanomechanical sensors to single out active small ligands for β2-microglobulin
Giulio Oliviero, Marcella Chiari, Ersilia De Lorenzi, Raffaella Colombo, Marina Cretich, Francesco Damin, Stefania Federici, Laura E. Depero, Paolo Bergese
Sensors and Actuators B Chemical, 2013
Role of nanomechanics in canonical and noncanonical pro-angiogenic ligand/VEGF receptor-2 activation
Daniele Maiolo, Stefania Mitola, Daria Leali, Giulio Oliviero, Cosetta Ravelli, Antonella Bugatti, Laura E. Depero, Marco Presta, Paolo Bergese
Journal of the American Chemical Society, 2012
On the thermodynamics of biomolecule surface transformations
Stefania Federici, Giulio Oliviero, Daniele Maiolo, Laura E. Depero, Italo Colombo, Paolo Bergese
Journal of Colloid and Interface Science, 2012
Nanomechanical recognition of N -methylammonium salts
Marco Dionisio, Giulio Oliviero, Daniela Menozzi, Stefania Federici, Roger M. Yebeutchou, Franz P. Schmidtchen, Enrico Dalcanale, Paolo Bergese
Journal of the American Chemical Society, 2012
Quantifying the nanomachinery of the nanoparticle-biomolecule interface
Helena de Puig, Stefania Federici, Salmaan H. Baxamusa, Paolo Bergese, Kimberly Hamad‐Schifferli
Small, 2011
On the difference of equilibrium constants of DNA hybridization in bulk solution and at the solid-solution interface
Giulio Oliviero, Stefania Federici, Paolo Colombi, Paolo Bergese
Journal of Molecular Recognition, 2011
Local order and non-linear optical properties in bulk nanostructured niobiosilicate glasses
A. Aronne, E. Fanelli, P. Pernice, M. Malvestuto, P. Bergese, E. Bontempi, P. Colombi, L.E. Depero, L. Bignardi, C. Giannetti, G. Ferrini, F. Parmigiani
Journal of Non Crystalline Solids, 2011
Nanoliter contact angle probes tumor angiogenic ligand-receptor protein interactions
Giulio Olivero, Daniele Maiolo, Daria Leali, Stefania Federici, Laura E. Depero, Marco Presta, Stefania Mitola, Paolo Bergese
Biosensors and Bioelectronics, 2010
Protein thin film machines
Stefania Federici, Giulio Oliviero, Kimberly Hamad-Schifferli, Paolo Bergese
Nanoscale, 2010
Corrigendum to "A biofunctional polymeric coating for microcantilever molecular recognition" [Anal. Chim. Acta 630 (2008) 161-167] (DOI:10.1016/j.aca.2008.09.069)
Giulio Oliviero, Paolo Bergese, Giancarlo Canavese, Marcella Chiari, Paolo Colombi, Marina Cretich, Francesco Damin, Sonia Fiorilli, Simone L. Marasso, Carlo Ricciardi, Paola Rivolo, Laura E. Depero
Analytica Chimica Acta, 2009
Exploiting surface plasmon resonance (SPR) technology for the identification of fibroblast growth factor-2 (FGF2) antagonists endowed with antiangiogenic activity
Marco Rusnati, Antonella Bugatti, Stefania Mitola, Daria Leali, Paolo Bergese, Laura E. Depero, Marco Presta
Sensors, 2009
Molecular recognition by contact angle: Proof of concept with DNA hybridization
Paolo Bergese, Giulio Oliviero, Italo Colombo, Laura E. Depero
Langmuir, 2009
ZnO whiskers and belts in chestnut husk-like structures: Synthesis and proof of chemomechanical transduction
Ivano Alessandri, Paolo Bergese, Laura E. Depero
Journal of Nanoscience and Nanotechnology, 2009
Self-assembled polystyrene nanospheres for the evaluation of atomic force microscopy tip curvature radius
P Colombi, I Alessandri, P Bergese, S Federici, L E Depero
Measurement Science and Technology, 2009
A biofunctional polymeric coating for microcantilever molecular recognition
Giulio Oliviero, Paolo Bergese, Giancarlo Canavese, Marcella Chiari, Paolo Colombi, Marina Cretich, Francesco Damin, Sonia Fiorilli, Simone L. Marasso, Carlo Ricciardi, Paola Rivolo, Laura E. Depero
Analytica Chimica Acta, 2008
Advances in parallel screening of drug candidates
Paolo Bergese, Marina Cretich, Claudio Oldani, Giulio Oliviero, Gabriele Di Carlo, Laura Depero, Marcella Chiari
Current Medicinal Chemistry, 2008
Thermodynamics of mechanical transduction of surface confined receptor/ligand reactions
Paolo Bergese, Giulio Oliviero, Ivano Alessandri, Laura E. Depero
Journal of Colloid and Interface Science, 2007
Atomic force microscopy evaluation of the effects of a novel antimicrobial multimeric peptide on Pseudomonas aeruginosa
Greta Rossetto, Paolo Bergese, Paolo Colombi, Laura E. Depero, Andrea Giuliani, Silvia F. Nicoletto, Giovanna Pirri
Nanomedicine Nanotechnology Biology and Medicine, 2007
Investigation of a biofunctional polymeric coating deposited onto silicon microcantilevers
P. Bergese, E. Bontempi, M. Chiari, P. Colombi, F. Damin, L.E. Depero, G. Oliviero, G. Pirri, M. Zucca
Applied Surface Science, 2007
High temperature X-ray diffraction investigation of an aluminium-silicon- corundum system
D. Garipoli, P. Bergese, E. Bontempi, M. Minicucci, A. Di Cicco, L. E. Depero
Zeitschrift Fur Kristallographie Supplement, 2007
Analysis of livestock DNA using nanotechnologies
R. Mazza, S. Tramontana, P. Bergese, G. Oliviero, L. Depero, P. Ajmone-Marsan
Italian Journal of Animal Science, 2007
Phase transformations in bulk nanostructured potassium niobiosilicate glasses
P. Bergese, I. Alessandri, E. Bontempi, L. E. Depero, A. Aronne, E. Fanelli, P. Pernice, T. Boffa Ballaran, N. Miyajima, V. N. Sigaev
Journal of Physical Chemistry B, 2006
A simple solution to systematic errors in density determination by X-ray reflectivity: The XRR-density evaluation (XRR-DE) method
P. Bergese, E. Bontempi, L.E. Depero
Applied Surface Science, 2006
Specific heat, polarization and heat conduction in microwave heating systems: A nonequilibrium thermodynamic point of view
Paolo Bergese
Acta Materialia, 2006
Laboratory microbeam analysis applied to cultural heritage studies
Dario Benedetti, Ivano Alessandri, Paolo Bergese, Elza Bontempi, Paolo Colombi, Donatella Garipoli, Roberta Pedrazzani, Paolo Zanola, Laura E. Depero
Microchimica Acta, 2006
Microstructure and morphology of nimesulide/crospovidone nanocomposites by Raman and electron microscopies
P. Bergese, I. Alessandri, I. Colombo, N. Coceani, L.E. Depero
Composites Part A Applied Science and Manufacturing, 2005
Thermal transformations and stability of organometallic materials with electrical and optical properties: The case of polycrystalline cis-[Ir(CO) 2Cl(C5H5N)]
P. Bergese, E. Bontempi, C. Dragonetti, D. Roberto, R. Ugo, I. Colombo, L. E. Depero
Journal of Physical Chemistry B, 2005
Melting of nanostructured drugs embedded into a polymeric matrix
P. Bergese, I. Colombo, D. Gervasoni, Laura E. Depero
Journal of Physical Chemistry B, 2004
Microwave generated nanocomposites for making insoluble drugs soluble
P. Bergese, I. Colombo, D. Gervasoni, L.E. Depero
Materials Science and Engineering C, 2003
Assessment of the X-ray diffraction-absorption method for quantitative analysis of largely amorphous pharmaceutical composites
P. Bergese, I. Colombo, D. Gervasoni, Laura E. Depero
Journal of Applied Crystallography, 2003
Microstructural investigation of nimesulide-crospovidone composites by X-ray diffraction and thermal analysis
P Bergese, E Bontempi, I Colombo, D Gervasoni, L.E Depero
Composites Science and Technology, 2003
High-resolution radon monitoring and hydrodynamics of Mount Vesuvius
Corrado Cigolini, Francesco Salierno, Gianpiero Gervino, Paolo Bergese, Ciro Marino, Massimo Russo, Paolo Prati, Vincenzo Ariola, Roberto Bonetti, Stefania Begnini
Geophysical Research Letters, 2001
Micro X-ray diffraction on capillary powder samples: A novel and effective technique for overcoming preferred orientation
P. Bergese, E. Bontempi, I. Colombo, L. E. Depero
Journal of Applied Crystallography, 2001

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