Dr Vaishali Ravindra Undale
@pharmacy.dypvp.edu.in
Professor Department of Pharmacology
Dr.D.Y.Patil Institue of Pharmaceutical Sciences and Research
EDUCATION
M Pharm, Ph D in pharmacology
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmacology, Toxicology and Pharmaceutics, Neuroscience, Drug Discovery, Pharmaceutical Science
Scopus Publications
Scopus Publications
Pratima Tambe, Vaishali Undale, Avinash Sanap, Ramesh Bhonde, and Nishant Mante
Elsevier BV
Akshay Narawade, Omkar Janjire, Vaishali Undale, Addepalli V, Suvarna Ingale, Pawan N Karwa, and Vrushali Neve
BSP Books Private Limited
Background: Nephrotoxicity is among the significant side effects and a dose-restriction mechanism for cisplatin treatment. According to recent studies, Oxidative stress and inflammation are the causative agents for acute renal failure on Cisplatin. Curcumin and resveratrol are considered to have potent anti-inflammatory and antioxidant effects. The current investigation aimed to determine whether a composite of curcumin and resveratrol attenuates nephrotoxicity caused by Cisplatin in Wistar rats. Method: The herbal compound was administered together with 5 mg/kg of cisplatin daily for 10 days with doses of 100 and 400 mg/kg. To evaluate renal damage, serum creatinine, nitrogen in blood urea, Creatinine, and levels of nitrite were measured. The levels of renal malondialdehyde, decreased glutathione levels, catalase, and also the superoxide dismutase enzyme were measured to quantify renal oxidative stress. Result: Significant oxidative stress, severe inflammation, and nephrotoxicity were all seen after a single dosage of cisplatin. In Wistar rats, a herbal composite treatment significantly reduced inflammation, improved renal function in a dose-dependent manner, reduced oxidative stress, and provided protection from the nephrotoxicity caused by Cisplatin. Conclusion: The herbal composite of curcumin - and - resveratrol was found to act as a therapeutic adjutant against Cisplatin nephrotoxicity.
Mahesh Asalkar, Hanif Shaikh, Priyanka V Mahadik, Parveen Shaikh, Anil Pardeshi, Shravani P Wakte, Krishna S Dakh, Vaishali R Undale, Anand S Kawade, and Chandrashekhar Upasani
Jaypee Brothers Medical Publishing
M. Nilavukkarasi, S. Vijayakumar, Pradnya Jagtap, Vaishali Undale, Nilambari Gurav, Shailendra Gurav, R. Mythili, Sandhanasamy Devanesan, Mohamad S. Alsalhi, and Woong Kim
Springer Science and Business Media LLC
Hanif Shaikh, Vaishali Undale, Anil Pardeshi, Shweta Jadhav, Pratiksha Jagtap, Parth Aphale, Anand Kawade, Mahesh Asalkar, Suneth Agampodi, Aman Upaganlawar,et al.
IP Innovative Publication Pvt Ltd
: This cross-sectional study was aimed to assess the knowledge, attitudes, awareness, facilitators, and barriers associated with the uptake of influenza vaccination during pregnancy in the urban area of Pune. : The study was conducted with 500 participants: 62 pregnant women and 438 postpartum women (total 524 were approached) attending Obstetrics and Gynaecology outpatient department (OPD) in a tertiary-care municipal hospital in Pune. The study-questionnaire collected data regarding socio-demographics general knowledge about influenza, attitude towards influenza vaccination, vaccine risk awareness, and its potential risk and benefits during pregnancy. The log-binomial regression models were used to examine the factors associated with influenza vaccination. : A good uptake of the vaccine (223/500, 44.6%) was found during current pregnancy of the participant; however, only 2.7% of study-participants vaccinated with influenza vaccine in their previous pregnancies. Also, there was poor awareness among all the participants regarding the safety of the seasonal influenza vaccine during pregnancy (19%, n=95/500). 11.2% (n=31/277) study-participants were found to be against the maternal influenza vaccination. Women receiving the vaccine against influenza were more likely to be previously informed of the recommendations from health-care providers about maternal influenza vaccination with odds ratio of 0.18 (95% CI, 0.04 to 0.8; p<0.00001), received the influenza vaccine in the past OR 8.27 (95% CI, 0.94 to 72.0), Z 1.09, p=0.05 and of having knowledge on influenza and its complications has more impact on influenza vaccination during pregnancy OR 14.4 (95% CI, 5.68 to 36.53), Z 5.6, p<0.0001 respectively.: This study concludes the vital causative factors for non-vaccination were provision of incomplete information or poor awareness about influenza vaccination (68.6%). There was poor knowledge among the pregnant women regarding the safety of maternal influenza vaccines. The recommendation from healthcare professionals is the major facilitators of decision-making by pregnant women for influenza vaccination during pregnancy.
Karishma Rathi, Ravindra Wavhale, Ritesh Bhole, Vaishali Undale, and Somdatta Chaudhari
FapUNIFESP (SciELO)
Alzheimer’s disease is a devastating neurodegenerative disorder characterized by memory loss and cognitive decline. New AD treatments are essential, and drug repositioning is a promising approach. In this study, we combined ligand-based and structure-based approaches to identify potential candidates among FDA-approved drugs for AD treatment. We used the human acetylcholinesterase receptor structure (PDB ID: 4EY7) and applied Rapid Overlay of Chemical Structures and Swiss Similarity for ligand-based screening.Computational shape-based screening revealed 20 out of 760 FDA approved drugs with promising structural similarity to Donepezil, an AD treatment AChE inhibitor and query molecule. The screened hits were further analyzed using docking analysis with Autodock Vina and Schrodinger glide. Predicted binding affinities of hits to AChE receptor guided prioritization of potential drug candidates. Doxazosin, Oxypertine, Cyclopenthiazide, Mestranol, and Terazosin exhibited favorable properties in shape similarity, docking energy, and molecular dynamics stability.Molecular dynamics simulations confirmed the stability of the complexes over 100 ns. Binding free energy analysis using MM-GBSA indicated favourable binding energies for the selected drugs. ADME, formulation studies offered insights into therapeutic applications and predicted toxicity.This comprehensive computational approach identified potential FDA-approved drugs (especially Doxazosin) as candidates for repurposing in AD treatment, warranting further investigation and clinical assessment.
Sanjeevani Shekhar Deshkar, Shubhangi Vitthal Shekade, Nilesh Raghu, and Vaishali Ravindra Undale
EManuscript Technologies
Aim/Background: Venlafaxine HCl (VEN) is an antidepressant drug with extensive first pass metabolism and low oral bioavailability. In the present study, a solid self-emulsifying formulation of Venlafaxine was developed and evaluated for in vitro and in vivo performance. Materials and Methods: Various oils, surfactants, and cosurfactants were screened for the solubility of Venlafaxine in them. Surfactants were further screened based on their ability to emulsify oils. For the SMEDDS formulation, Caprol PGE 860 was selected as oil base, Tween 20 as a surfactant and propylene glycol as a co-surfactant. Pseudoternary phase diagrams were plotted with different concentrations of these three components with water to identify microemulsion area. The optimized formulation containing Venlafaxine (5.9%), Caprol PEG 860 (9.3%), Tween 20 (42.4%) and propylene glycol (42.4%) was converted into solid SMEDDS (S-SMEDDS) by using Spray Drying method and evaluated for the flow property, drug content, drug release, DSC, XRD, SEM and in vivo antidepressant activity. Results: The optimized SMEDDS formulation demonstrated globule size of 21 nm with no signs of precipitation upon dilution, was thermodynamically stable, and released more than 90% of Venlafaxine in 30 min. S-SMEDDS formulation was free flowing and SEM and PXRD studies revealed amorphous state of S-SMEDDS. In vivo antidepressant activity by forced swim test and anti-anxiety test by EPM maze test in rats demonstrated significant efficacy of SMEDDS formulation over plain drug. Conclusion: A self-emulsifying formulation of Venlafaxine was successfully developed and showed improved antidepressant activity in comparison to pure drug, thus can serve as potential alternative to existing oral formulations.
Sunil Shewale, Vaishali Undale, V. Addepalli, Vrushali Bhalchim, Shivani Desai, Vikas Wawale, Maruti Shelar, Shubham Padole, Shital Satone, Shweta Lembhe,et al.
Informatics Publishing Limited
Sansevieria cylindrica Bojer ex. Hook (Asparagaceae) and Plumeria obtusa L. (Apocynaceae) are useful traditional medicines. The present study assessed the toxicity of a combination of hydro-alcoholic extract of aerial parts of both these plants through repeat administration in Wistar albino rats. This study is an extension of an acute toxicity study, wherein female rats were orally administered with a single dose of 2000mg/kg of both plant extracts. Sub-acute toxicity of both plants (200 and 400 mg/kg p.o.) was studied by daily dosing of Wistar rats of both sexes for 28 days. Toxicity, mortality, body weight changes, food and water intake, and neurological assessments were recorded. Hematological, biochemical, and urine analysis was done before and after dosing on day 29. Absolute and relative organ weight and histological evaluation were performed on day 29. The acute toxicity study revealed no lethal effects indicating that LD50 is greater than 2000mg/ kg. The sub-acute study observed a significant increase (p < 0.05) in body weight, feed, and water intake. The body weight gain in rats from the treatment group was non-significant (p > 0.05) compared to the control group. A significant (p < 0.05) increase in lymphocytes, granulocytes, RBC, platelet, creatinine, albumin, triglycerides, and alkaline phosphatase were also observed. The evaluation of different neurobehavior parameters showed a significant increase (p < 0.05). Histopathological analysis showed mild liver distortion in male rats in both treatment groups. Most of the significant observations were considered incidental findings indicative of low toxicity of plant extract during long-term use.
Hanif Shaikh, Pranesha Koli, Vaishali Undale, Anil Pardeshi, Mahesh Asalkar, Sushant Sahastrabuddhe, Anand Kawade, and Chandrashekhar Upasani
MDPI AG
Background: Maternal influenza vaccination provides effective protection against influenza infections in pregnant women and their newborns. In India, the influenza vaccine has not yet been offered through immunization programs, owing to the lack of sufficient safety data for pregnant Indian women. Methods: This cross-sectional observational study enrolled 558 women admitted to the obstetrics ward of a civic hospital in Pune. Study-related information was obtained from the participants through hospital records and interviews using structured questionnaires. Univariate and multivariable analysis was used, and the chi-square test with adjusted odds ratio was estimated to account for vaccine exposure and the temporal nature of each outcome, respectively. Results: Women not vaccinated against influenza during pregnancy had a higher risk of delivering very LBW infants, and possible protective effects were suggested (AOR 2.29, 95% CI 1.03 to 5.58, p = 0.03). No association was observed between maternal influenza vaccination for Caesarean section (LSCS) (AOR 0.97, 95% CI, 0.78, 1.85), stillbirth (AOR 1.8, 95% CI 0.18, 24.64) and NICU admission (AOR, 0.87, 0.29 to 2.85), and congenital anomaly (AOR, 0.81, 0.10 to 3.87). Interpretation: These results show that the influenza vaccine administered during pregnancy is safe and might lower the risk of negative birth outcomes.
Sunil Shewale, Vaishali Ravindra Undale, Maruti Shelar, Bhagyashri Warude, Mohini Kuchekar, Vrushali Bhalchim, Shital Satone, Shweta Lembhe, and Sachin Gundecha
Defence Scientific Information and Documentation Centre
Plumeria obtuse L. (Apocynaceae) is an ornate outdoor plant. The plant was traditionally used during accidentalinjuries. However, the pharmacognosy of this plant is very poorly explored. Therefore, we have conducted this study to assess the distinctive qualities of the P. obtusa. To investigate P. obtusa seed pods’ preliminary pharmacognostic, physical-chemical, phytochemical, microscopic, and phytoconstituent potential. Initially, the shape and microscopic characteristics of plant seed pods were assessed. Physicochemical analysis was used for the standardization. Utilizing several chemical techniques, phytoconstituents were evaluated qualitatively. This was followed by quantitative estimation and analytical profiling of various phytoconstituents. The basic characteristics of the seed pod have been documented by macroscopy to be its brown color, sweet aroma, bitter flavor, coarse texture, and rough fracture. Microscopy showed the existence of vascular bundles, lignified fibers, calcium oxalate crystals and arteries. The results of the physicochemical analysis revealed no foreign organic matter, 2.8 % weight-average moisture content and a high total ash value of 14.80 compared to an acid insoluble ash value of 0.70, which indicated that there was less inorganic matter in the plant. The extractive values were 3.93, 6.03 and 10.16 % w/w for water soluble, alcohol soluble and hydro-alcoholic soluble extracts respectively. Flavonoids, glycosides, saponins, phenolic constituents, tannins and carbohydrates were found during early phytochemical analysis. Instrumental analysis has given an idea about functional groups present whereas GCMS technique helped in identification of phytoconstituents. The results of this study can be significantly used as a reference support for quality control and standardization of P. obtusa and preparation of a monograph of plant.
Khushboo Borah Slater, Daniel Kim, Pooja Chand, Ye Xu, Hanif Shaikh, and Vaishali Undale
MDPI AG
Tuberculosis (TB) is one of the ten infectious diseases that cause the highest amount of human mortality and morbidity. This infection, which is caused by a single pathogen, Mycobacterium tuberculosis, kills over a million people every year. There is an emerging problem of antimicrobial resistance in TB that needs urgent treatment and management. Tuberculosis treatment is complicated by its complex drug regimen, its lengthy duration and the serious side-effects caused by the drugs required. There are a number of critical issues around drug delivery and subsequent intracellular bacterial clearance. Drugs have a short lifespan in systemic circulation, which limits their activity. Nanomedicine in TB is an emerging research area which offers the potential of effective drug delivery using nanoparticles and a reduction in drug doses and side-effects to improve patient compliance with the treatment and enhance their recovery. Here, we provide a minireview of anti-TB treatment, research progress on nanomedicine and the prospects for future applications in developing innovative therapies.
K. Rathi, R. Wavhale, V. Undale V., R. Bhole, and S. Dherange
Medical Informational Agency Publishers
Alzheimer’s disease (AD) is a progressive neurological disease that causes cognitive impairment in old aged persons. It is the cause of a wide spectrum of neurodegenerative disturbances including tauopathies, which are responsible for progressive neuronal degeneration and impaired cognitive functions. Although drug discovery researchers and pharmaceutical companies are meticulously working to develop novel drugs for AD, establishing their safety and efficacy proofs are major challenges for them. In this review, we have discussed about AD and its causes mainly focusing on molecular targets with their physiological and pathophysiological roles, therapeutic approaches, and their future perspectives. We have compiled the information about novel and promising drug targets and lead data bases that will help to select appropriate target and design novel drug molecules for the treatment of Alzheimer.
Madhura Shekatkar, Supriya Kheur, Avinash Sanap, Vaishali Undale, Avinash Kharat, Vrushali Bhalchim, Arati Dubewar, and Ramesh Bhonde
Springer Science and Business Media LLC
Sunil Shewale, Vaishali Undale, Vrushali Bhalchim, Shivani Desai, Maruti Shelar, Shubham Padole, Sohan Chitlange, Vikas Wawale, Sameer Parekh, and Pramod Pujari
Informatics Publishing Limited
<em>Sansevieria cylindrica</em> (SC) Bojer ex Hook. (Asparagaceae) and <em>Plumeria obtusa</em> (PO) L. (Apocynaceae) are indoor and outdoor ornamental plants respectively. These plants are traditionally used by the local healers during accidental injuries. However, their toxicological properties are very poorly explored over folkloric usage. Therefore, the present study evaluated the toxic potencies of SC leaves and PO seed Hydro-Alcoholic Extract (SCPOHAE) through acute oral dose (14-days) administration in female Wistar rats. Safety of the SCPOHAE was evaluated as per Organization for Economic Co-operation and Development (OECD) Acute Oral Toxicity study guidelines 423. The female Wistar rats were divided into three groups (n=3). A single oral dose of 2000 mg/kg of body weight of individual extract and 1:1 blend was administered to each animal. The animals were closely observed for clinical signs, neurobehavioral changes, morbidity, and mortality if any for the first half an hour and then every hour for the first four hours followed by observation every 24–hours for 14 days. Changes in food and water consumption, body weight were monitored daily during the study. On day 1 and day 15 blood samples were collected to evaluate changes in the hematology and biochemistry parameters. The urine samples were also collected for urine analysis parameters. Animals were sacrificed on day 15 and organ samples of liver and kidney were collected for histopathological findings. The SCPOHAE individually and also as 1:1 blend at the limit dose (2000 mg/kg, body weight) did not cause death and did not induce any remarkable and abnormal clinical signs, indicative of systemic toxicity, in rats during the treatment period of 14–days. The statistically non-significant small differences in the body weight were observed. <strong>Conclusion:</strong> The oral administration of SCPOHAE did not cause any systemic toxic effects. In conclusion, the No-observed-Adverse-Effect Level (NOAEL) of these extracts in rats was found to be greater than 2000 mg/kg.
Mayuri Gurav and Vaishali Undale
Springer Nature Singapore
, Shridhar PANDYA, Chetan SAVALIYA, , Dheeraj NAGORE, , Vaishali UNDALE, , Shubhada KHADE, ,et al.
CMMB-Colegiul Medicilor Municipiul Bucuresti
Osteoarthritis (OA) is most prevalent joint disease and major contributor to non-fatal burden in India, with prevalence rates of 22% - 39%. The use of conventional medication can be associated with insufficient clinical management and serious side effects. The present study aims to evaluate the anti-arthritic activity and chondrocytes protection and regeneration potential of Joint Support Product (JSP) in monosodium Iodoacetate induced Osteoarthritis rat model. Pain threshold, knee joint swelling, Blood inflammatory parameters like Tissue necrosis factor, Interlukin-6, Leukotriene B 4, C- reactive protein and arthritic biomarkers Matrix metalloproteinase-13 and Cartilage Oligomeric Matrix Protein, were estimated. Also Radiographic and histopathological evaluation were done to estimate the severity of OA. Treatment of JSP demonstrated significant increase in pain threshold by 68 % and decrease in knee joint swelling by 92 %. The inflammatory markers decreased significantly (p< .00001) after treatment with JSP. The Tissue necrosis factor decreased by 55 %, Interlukin-6 by 61%, Leukotriene B 4 by 57%, C- reactive protein by 69 % and arthritic biomarkers - Matrix metalloproteinase by 88% and Cartilage Oligomeric Matrix Protein by 37% as compared to disease control rats. Also the radiological evaluation and gross histopathology of rats showed improved chondrocytes structure. Thus JSP’s analgesic, anti-inflammatory, chondrocyte regeneration, and chondroprotective properties have therefore demonstrated an anti-arthritic impact.
Sunil Shewale, Vaishali Undale, Maruti Shelar, Vrushali Bhalchim, Chandramohan Panchal, and Sachin Gundecha
Elsevier BV
Kajal Jirapure and Vaishali Undale
Bentham Science Publishers Ltd.
Abstract: Diabetes mellitus is a chronic illness with a variety of causes and pathophysiology. For the management of diabetes, various synthetic antidiabetic drugs are available. Still, people prefer complementary and alternative therapies as well as traditional herbal home remedies because they are perceived to be free of side effects and generally recognized as safe due to their natural origin. Hence, worldwide, the majority of the population is consuming herbs and/or herbal products in their daily routine. It has been observed that individuals with diabetes also consume herbs/herbal products either with or without medical supervision. This co-consumption of antidiabetic medications and herb/herbal products may result in herb-drug interactions, which might be potentially beneficial or harmful or, in some cases, even fatal. Most of the times, these interactions remain unnoticed or undiagnosed due to lack of knowledge and awareness about them. In this review, the authors have summarized some important aspects related to the herb-drug interaction (HDI), which include methods for prediction and mechanism of HDI (pharmacokinetic and pharmacodynamic) and also the clinical and experimental literature on herb-drug interactions (HDI) in the treatment of diabetes. Authors have attempted to categorize the interactions between oral hypoglycemic agents and various herbs as beneficial or harmful based on the results reported in the original research work.
Shivani V. Dhokne, Vaishali R. Undale, Dinesh Chandra Agrawal, and Sharad D. Pawar
Springer Singapore
Vaishali. R. Undale, Asawari S. Thakare, and Pooja V. Katkar
A and V Publications
Vaishali Ravindra UNDALE, Suchita GUPTA, and Kedar LAKHADIVE
Galenos Yayinevi
Antimicrobial resistance (AMR) is the phenomenon developed by microorganism on exposure to antimicrobial agents, making them unresponsive. Development of microbial confrontation is a severe rising risk to global community well-being as treatment in addition, management of such resistant microbial infections is difficult and challenging. The situation requires action across all government sectors and society. The change in the molecular target on which antimicrobial drugs act is one of the key mechanisms behind AMR. One of the approaches to battle with AMR can be exploring newer molecular targets in microbes and discovering new molecules accordingly. There are various examples of novel targets such as biomolecules involving in biosynthesis of cell wall, biosynthesis of aromatic amino acid, cell disunion, biosynthesis of fatty acid, and isoprenoid biosynthesis and tRNA synthetases. Fatty acid biosynthesis (FAB) and their enzymes among all the above is the more appealing target for the advancement of new antimicrobial agents. Number of promising inhibitors have been developed for bacterial fatty acid synthesis (FAS) and also few of them are clinically used. Some of these potential inhibitors are found to be used in development of new antibacterial as a lead compound and have been discovered from high throughput screening processes like Platencimycin and their analogue, Platencin. The review majorly encompasses bacterial FAB in type II FAS system and potential inhibitors with respective targets of novel antibacterial.
Bhavika D. Satone, Atul A. Deshmukh, and Vaishali R. Undale
A and V Publications
Vaishali. R. Undale, Sujata S. Kurkute, and Srutuja S. Jadhav
A and V Publications