Sexual dysfunction and quality of life in postmenopausal type 2 diabetes mellitus: clinical and laboratory factors Sandra Ester Alves, Márcia Silva Queiroz Climacteric, 2026 OBJECTIVE: This study aimed to evaluate female sexual dysfunction (FSD) in postmenopausal women with and without type 2 diabetes (T2D) and its relationship with clinical, laboratory and socioeconomic parameters and quality of life (QoL). METHOD: This cross-sectional study enrolled postmenopausal women with and without T2D not taking hormone replacement. Clinical and laboratory factors were assessed, and participants answered cardiovascular risk, socioeconomic, Short Form Health Survey 36 (SF-36) and Female Sexual Function Index (FSFI) questionnaires. RESULTS: = 110) were similar in age, marital status, race/ethnicity, employment status, alcohol use and body mass index. Women with T2D showed higher glycemia, glycated hemoglobin, cholesterol, thyroid-stimulating hormone and cardiovascular risk factors. SF-36 scores were significantly lower in women with T2D and sexual dysfunction compared to those without diabetes. FSFI scores below 26.55 were associated with higher odds of FSD with increasing age, whereas vitality showed an inverse association. CONCLUSION: In postmenopausal women with T2D, reduced FSFI scores along with hypertension, hypothyroidism and elevated cardiometabolic risk were linked to poorer QoL. Age increased the odds of FSD, while vitality decreased them. These results underscore the multifactorial interaction of T2D, comorbidities and menopause in women's sexual health and well-being.
Integrating Nursing–Teleophthalmology Improves Diabetic Retinopathy Screening in Primary Healthcare, Reducing Unnecessary Referrals to Specialist Healthcare Jacira Xavier de Carvalho, Fernando K. Malerbi, Silvia Ferreira Bortoto, Mozania Reis de Matos, Cristiane das Graças Dias Cavalcante, Elenilda Almeida Silva Andrade, Gabriela Bonfim Vieira, Márcia Silva Queiroz International Journal of Nursing Practice, 2025 AimThis work aimed to evaluate the use of teleophthalmology by a primary healthcare nursing team for the diagnosis and referral for diabetic retinopathy to specialized healthcare in relation to numbers referred for specialized healthcare.MethodsIn this quantitative, cross‐sectional service evaluation study, participants with type 2 diabetes mellitus underwent a fundoscopy examination between February and June 2020. Using a portable retinal camera attached to a smartphone, nurses acquired fundus images that were stored on a cloud platform, enabling remote reading by a retinal specialist. The study was conducted at a primary healthcare urban centre on the outskirts of São Paulo, Brazil.ResultsThe study enrolled 779 participants, of whom 150 were identified as having diabetic retinopathy present; in another 434, evidence of diabetic retinopathy was absent, and 195 individuals (25%) were classified as having ungradable images. In total, 345 participants were referred for specialized appraisal, 150 of whom due to evidence of diabetic retinopathy and for another 195 participants owing to ungradable images. Thus, more than half of the imaged participants (56%) were not eligible for referral to specialist healthcare and remained treated in primary care.ConclusionsNursing–teleophthalmology integration reduced specialized healthcare referral numbers by more than half. This approach contributed to better triage with a more robust evaluation for diabetic retinopathy diagnostic suspicion, reducing unnecessary referral.
Screen Time and Hours of Sleep Influence the Estimate Risk of Diabetes Mellitus and Metabolic Syndrome in Healthy Young Males Carolina Costa-Santos, Gabriella Guarilha Costa Dias Mattar, Ronan Antonio Fuziwara, Jorge Alexandre de Araújo Peres, Márcia Silva Queiroz Metabolic Syndrome and Related Disorders, 2024 Objective: Screen time (ST) has shown negative effects on physical and mental health, with an increase in the prevalence of overweight, metabolic syndrome (MetS), and obesity. The time spent in front of the screens was also associated with higher odds of selecting indicators of cardiometabolic disease in adulthood. In view of this, the aim of this study was to identify the risk of MetS and type 2 diabetes mellitus (T2DM) in healthy young males and relate it to ST and sleep time. Methods: We evaluated physical and laboratory characteristics, dichotomous diagnosis criteria, and continuous scores to assess MetS and Finnish Diabetes Risk Score questionnaire to measure the T2DM risk. Results: The means of MetS dichotomous and continuous severity criteria, among individuals with <7 hr of sleep, were higher than those with adequate sleep. We did not observe a direct impact of ST on the risk of MetS; nevertheless, >8 hr of ST increased 1.22 points in the T2DM risk. Conclusion: Excessive ST increased the risk of T2DM, but not of MetS. Moreover, sleeping <7 hr was associated with a higher mean of dichotomous and continuous severity criteria for MetS.
Advances in separation methods for the use of platelet-rich fibrin in tissue repair: an integrative review General Dentistry, 2023
Could CA 19-9 be a useful biomarker in the diagnosis, prognosis, and prediction of adequate relief in lower urinary tract obstructions? MARCIA EMILIA FRANCISCO SHIDA, HUMBERTO DELLÊ, MARCIA SILVA QUEIROZ Revista do Colegio Brasileiro De Cirurgioes, 2022 Introduction: posterior urethral valves represent an important cause of childhood chronic kidney disease. The identification of biomarkers that indicate early kidney damage and even adequate clearance could reduce how many patients head towards kidney failure. Objective: this study evaluated how this easy-analysis biomarker (CA 19-9) could help identifying potential renal damage and adequate clearance in obstructive uropathies. Methods: 46 female Wistar rats were divided into 5 groups, with different patterns of partial urinary tract obstruction: group control; group OIV: infravesical obstruction; group OIVd: infravesical obstruction with reversion, obstruction relief 7 postoperative days later; group OUu: unilateral ureteral obstruction; group OUb: bilateral ureteral obstruction. The CA 19-9s performance was compared to another biomarker: Ngal. Determination of basal CA 19-9 and Ngal in urine and blood and serum creatinine levels was performed in the rats prior to surgery (T0) and after 14 days (T1). Group OIVd underwent intermediate (Ti) collection before clearance. Results: the urinary concentration of CA 19-9 increased in groups OIV, OIVd and OUb; elevation at T1 and Ti, reached statistical significance compared to the T0 value (p<0,05). Changes in urinary CA 19-9 were more expressive in infravesical obstruction groups (AUC 0.81). Obstruction relief in group OIVd promoted significant urinary CA 19-9 reduction (p<0,05) in the final evaluation. Conclusions: CA 19-9 urinary concentration increased in partial urinary tract obstruction. Its best performance was in the bladder neck obstruction group, in which the elevation was detected early (6 days after infravesical obstruction) and the CA19-9 urinary concentration declined after clearance.
Serum albumin modified by carbamoylation impairs macrophage cholesterol efflux in diabetic kidney disease Aécio Lopes de Araújo Lira, Monique de Fátima Mello Santana, Raphael de Souza Pinto, Carlos André Minanni, Rodrigo Tallada Iborra, Adriana Machado Saldiba de Lima, Maria Lúcia Correa-Giannella, Marisa Passarelli, Márcia Silva Queiroz Journal of Diabetes and Its Complications, 2021 BACKGROUND AND AIMS Abnormalities in lipid metabolism, accumulation of uremic toxins and advanced glycation end products may contribute to worsening atherosclerosis. This study characterized the glycation and carbamoylation profile of serum albumin isolated from individuals with diabetic kidney disease and its influence on cholesterol efflux. MATERIAL AND METHODS 49 patients with type 2 diabetes (T2DM) and different eGFR evaluated glycation and carbamoylation profile by measurement of carboxymethyl lysine (CML) and carbamoylated proteins (CBL) in plasma by ELISA, homocitrulline (HCit) in plasma by colorimetry. In the isolated albumins, we quantified CBL (ELISA) and total AGE and pentosidine by fluorescence. Macrophages were treated with albumin isolated, and 14C-Cholesterol efflux mediated by HDL2 or HDL3 was measured. Kruskal-Wallis test, Jonckheere-Terpstra test and Brunner's posttest were used for comparisons among groups. RESULTS Determination of CML, HCit, CBL in plasma, as total AGE and pentosidine in albumins, did not differ between groups; however, CBL in the isolated albumins was higher in the more advanced stages of CKD (p=0.0414). There was reduction in the 14C-cholesterol efflux after treatment for 18h with albumin isolated from patients with eGFR<60mL/min/1.73m2 compared with control group mediated by HDL2 (p=0.0288) and HDL3 (p<0.0001), as well as when compared with eGFR ≥60mL/min/1.73m2 per HDL2 (p=0.0001) and HDL3 (p<0.0001). Treatment for 48h showed that eGFR<15mL/min/1.73m2 had a lower percentage of 14C-cholesterol efflux mediated by HDL2 compared to control and other CKD groups (p=0.0274). CONCLUSIONS Albumins isolated from individuals with T2DM and eGFR<60mL/min/1.73m2 suffer greater carbamoylation, and they impair the cholesterol efflux mediated by HDL2 and HDL3. In turn, this could promote lipids accumulation in macrophages and disorders in reverse cholesterol transport.
Variants in HSD11B1 gene modulate susceptibility to diabetes kidney disease and to insulin resistance in type 1 diabetes Rosana Cristina Mori, Daniele Pereira Santos‐Bezerra, Tatiana Souza Pelaes, Sharon Nina Admoni, Ricardo Vessoni Perez, Maria Beatriz Monteiro, Cleide Guimarães Machado, Marcia Silva Queiroz, Ubiratan Fabres Machado, Maria Lúcia Correa‐Giannella Diabetes Metabolism Research and Reviews, 2021 Abstract Background and aim 11β‐Hydroxysteroid dehydrogenase 1 has been implicated in insulin resistance (IR) in the setting of metabolic disorders, and single nucleotide polymorphisms (SNPs) in its encoding gene ( HSD11B1 ) have been associated with type 2 diabetes and metabolic syndrome. In type 1 diabetes (T1D), IR has been related to the development of chronic complications. We investigated the association of HSD11B1 SNPs with microvascular complications and with IR in a Brazilian cohort of T1D individuals. Materials and methods Five SNPs were genotyped in 466 T1D individuals (57% women; median of 37 years old, diabetes duration of 25 years and HbA1c of 8.4%). Results The minor allele T of rs11799643 was nominally associated with diabetic retinopathy (OR = 0.52; confidence interval [CI] 95% = 0.28‐0.96; P = .036). The minor allele C of rs17389016 was nominally associated with overt diabetic kidney disease (DKD) (OR = 1.90; CI 95% = 1.07‐3.37; P = .028). A follow‐up study revealed that 29% of the individuals lost ≥5 mL min −1 × 1.73 m 2 per year of the estimated glomerular filtration rate (eGFR). In these individuals (eGFR decliners), C allele of rs17389016 was more frequent than in non‐decliners (OR = 2.10; CI 95% = 1.14‐3.89; P = .018). Finally, minor allele T of rs846906 associated with higher prevalence of arterial hypertension, higher body mass index and waist circumference, thus conferring risk to a lower estimated glucose disposal rate, a surrogate marker of insulin sensitivity (OR = 1.23; CI 95% = 1.06‐1.42; P = .004). Conclusion SNPs in the HSD11B1 gene may confer susceptibility to DKD and to IR in T1D individuals.
Genetic variants in DNMT1 and the risk of cardiac autonomic neuropathy in women with type 1 diabetes Daniele Pereira Santos‐Bezerra, Sharon Nina Admoni, Rosana Cristina Mori, Tatiana Souza Pelaes, Ricardo Vesoni Perez, Cleide Guimarães Machado, Maria Beatriz Monteiro, Maria Candida Parisi, Elizabeth Joao Pavin, Marcia Silva Queiroz, Marisa Passarelli, Ubiratan Fabres Machado, Maria Lucia Correa‐Giannella Journal of Diabetes Investigation, 2019
Interactions between kidney disease and diabetes: Dangerous liaisons Roberto Pecoits-Filho, Hugo Abensur, Carolina C. R. Betônico, Alisson Diego Machado, Erika B. Parente, Márcia Queiroz, João Eduardo Nunes Salles, Silvia Titan, Sergio Vencio Diabetology and Metabolic Syndrome, 2016
