AMELIORATIVE EFFECTS OF SELECTIVE PDE-10 INHIBITORS AGAINST KETAMINE-MEDIATED SCHIZOPHRENIC OUTCOMES IN MICE RUCHIKA SRIVASTAVA, PRABHAT SINGH, AJEET Asian Journal of Pharmaceutical and Clinical Research, 2026 Objectives: Schizophrenia (SCZ) is characterized by significant impairments in perception and cognitive flexibility. Making accurate plans for therapy demands a deeper comprehension of the brain mechanisms behind these disorders. The purpose of our investigation is to analyze the protective effects of papaverine, a phosphodiesterase-10 inhibitor, on ketamine-induced SCZ -like behavioral and biochemical alterations in mice. Methods: For 10 consecutive days, mice were exposed to ketamine (30 mg/kg; i.p.) to develop a SCZ-like phenotype. Various behavioral tests, including social interactions, catalepsy, cognitive impairment (Morris water maze), locomotor and anxiety (open field test), and immobility duration (forced swim test), were assessed. Biochemicals (acetylcholinesterase [AChE] activity, glutathione, and lipid peroxides), and histopathological alterations were also investigated. In this study, papaverine (30 mg/kg; i.p.) and clozapine (7.5 mg/kg p.o.) served as test and standard, respectively. Results were statistically analyzed and “one-way ANOVA” was performed, and Tukey’s multiple comparison test was subsequently applied.” Results: After 28 days of ketamine therapy, significant (p≤0.05) behavioral alterations have been noted, including increased immobility duration, altered locomotor and anxiety-like behaviors, social interactions, cognitive impairment, and catalepsy. Significant alterations in histopathology, AChE activity, and oxidative stress (increased lipid peroxides and lower glutathione) were also observed in mice treated with ketamine. Treatment with clozapine and papaverine considerably (p≤0.05) improved the biochemical changes, behavioral problems, and histological changes. Conclusion: We may conclude that papaverine may have neurodefensive effects against ketamine-induced SCZ in mice based on behavioral, histological, and biochemical observations.
Neurodegeneration and synaptic impairments mediated by chronic light-induced circadian rhythm and molecular signaling disruption: an insight beyond vision Rajat Dumka, Prabhat Singh, Vishal Biswkarma, Lubhan Singh, Swati Wadhawan Biological Rhythm Research, 2026 Light not only provides visual information but also constitutes a powerful modulator of non-visual functions, including improvement of alertness and performance on several cognitive tasks. Over the last two decades, growing evidence has also shown that exposure to light from artificial sources is considered a pollutant for human health. The objective of this review is to update researchers in understanding the role of chronic light exposure and the mechanisms involved in neurological alteration. This review focuses on the role of lighting conditions in affecting non-visual consequences, especially the brain (learning and memory). The mechanistic approaches have been included and explained how chronic light induces neurological alterations in animal models. The review highlighted the impact and evidence of therapeutic alternatives evaluated and paucities in the management of neurodegenerative changes caused by chronic light exposure in preclinical studies. Beyond vision, the findings of the literature review indicated that chronic light exposure with different types, intensities, and exposure times leads to neurodegeneration and synaptic impairments via circadian rhythm and molecular signaling alterations. As for management, there are still many paucities. So, it becomes a major area for studies to be conducted as light pollution is increasing at its peak.
Therapeutic applications of rutin for diabetes mellitus and its complications Natural Therapeutics for Diabetes Mellitus Medicinal Plants Phytochemicals and Bioactive Compounds, 2025
PROTECTIVE EFFECT OF CILOSTAZOL AGAINST KETAMINE-INDUCED BIOCHEMICAL AND BEHAVIORAL PHENOTYPE OF SCHIZOPHRENIA IN MICE RUCHIKA SRIVASTAVA, PRABHAT SINGH, AJEET Asian Journal of Pharmaceutical and Clinical Research, 2025 Objective: Schizophrenia (SCZ), a mental illness affecting 1% of the world population, is characterized by extensive structural and functional brain changes. It is brought on by a confluence of psychological, environmental, and hereditary variables. It is frequently coexisted with other diseases, lowering the quality of life and increasing the risk of early death. The objective of this research is to explored the potential of cilostazol (Phosphodiesterase-3 inhibitor) in ketamine (KET)-induced SCZ -like behavioral and biochemical alterations in mice. Methods: In mice, SCZ was induced by injecting KET (30 mg/kg; i.p.) for 10 days in a row. Different behavioral parameters such as immobility time (Forced swim test), locomotor and anxiety (open field test), cognitive dysfunction (Morris water maze), social interactions, and catalepsy were examined. Histopathological and biochemical changes (lipid peroxides, glutathione [GSH], acetylcholinesterase [AChE] activity) were also examined. Cilostazol (25 and 50 mg/kg; p.o.) as a test and clozapine (7.5 mg/kg p.o.), as a standard drug were used in this investigation. Tukey’s multiple comparison test and one-way analysis of variance were used for statistical analysis of all the findings. p<0.050 was regarded as statistically significant. Results: Significant (p<0.05) behavioral changes have been observed following 28 days of KET treatment (increased immobility time, impaired locomotor and anxiety-like behaviors, cognitive dysfunction, social interactions, and catalepsy). Increased oxidative stress (higher lipid peroxides and decreased GSH), AChE activity, and histopathological changes were also noted significantly in KET -treated mice. Cilostazol and clozapine treatment significantly (p<0.05) corrected the histological changes, biochemical alterations, and behavioral problems. Conclusion: As per the behavioral, histopathological, and biochemical outcomes, we can draw a conclusion that cilostazol may provide neurodefensive effects against KET -induced SCZ in mice.
Exploring Nature's Pharmacy: A Comprehensive Review of Herbal Plants with Neuroprotective Properties Dipesh Prajapati, Prabhat Singh Current Green Chemistry, 2025 Neurodegenerative disorders, such as Alzheimer's, Parkinson's, and Huntington's, are an increasing health concern worldwide due to their progressive nature and limited therapeutic choices. In search of innovative treatment techniques, herbal plants have received considerable attention due to their possible neuroprotective characteristics. For the literature review, several databases are used like Science Direct, PubMed, Springer, Frontiers, MDPI, Wiley, and Elsevier. This article offers a complete assessment of the neuroprotective properties of several herbal plants in preclinical and clinical research. This article discussed the active components, modes of action, and therapeutic potential of selected medicinal plants, including Ginkgo biloba, Bacopa monnieri, Curcuma longa, Panax ginseng, and Withania somnifera. These plants have a variety of neuroprotective properties, including antioxidant, anti-inflammatory, anti-apoptotic, and neurogenesis-promoting properties. Additionally, this review emphasizes the synergistic benefits reported when employing mixtures of these plants or combining them with conventional therapies. Despite encouraging results, existing research is sometimes restricted by small sample numbers, diversity in study designs, and lack of uniform dosing. Future studies should overcome these limitations through well-designed clinical studies and standardized extraction processes to fully understand the neuroprotective potential of these herbal plants. This review emphasizes the importance of incorporating herbal medicines into the development of novel treatments for neurodegenerative illnesses.
Cerebroprotective Potential of Androgen Receptors in Ischemic Postconditioning against Cerebral Ischemia/Reperfusion-Induced Neurodegenerative Changes Prabhat Singh, Surbhi Gupta, Bhupesh Sharma, Lubhan Singh, Rani Bansal, Mamta Gupta Central Nervous System Agents in Medicinal Chemistry, 2025 Background and Objective: In stroke, reperfusion of blood to the cerebral ischemic area following sustained ischemia further exacerbates tissue damage, identified as cerebral ischemia and reperfusion (I/R) insult. Ischemic post-conditioning (IPoC) appears to offer benefits against I/R injury. The cascade of androgen receptors (ARs) has a vital role in cerebral stroke; however, its neurodefensive function in IPoC is unclear. This investigation aimed to explore the involvement of ARs in IPoC in cerebral I/R insult in rats. Methods: Global cerebral ischemia/reperfusion (GCI/R) insult in experimental animals was provoked by 10 minutes of obstruction of the bilateral carotid arteries after reperfusion for 24 hours. IPoC was carried out by providing a triad of I/R insults with a gap of 10 minutes of GCI after 24 hours of reperfusion. Lateral push, inclined beam, rota rod, hanging wire, and Morris-water maze experimentations were conducted on animals to determine motor control and cognitive functions (learning and memory). Cerebral oxidative damage markers (raised lipid peroxidation and reduced glutathione levels), acetylcholinesterase (AChE) activity, inflammatory indicators (interleukin-6, interleukin-10, tumor necrosis factor-α, and myeloperoxidase), infarction, and histopathological alterations were also assessed. Results: Animals with I/R exhibited reduced motor function and memory along with raised cerebral oxidative damage, AChE activity, inflammation, infarction, and histopathological alterations. IPoC after ischemic events recuperated the damaging outcomes of I/R insult. 60 minutes before cerebral ischemia, pretreatment with testosterone mimicked the neurodefensive outcomes of IPoC. However, neuroprotective outcomes developed by IPoC were diminished by flutamide (ARs antagonist) pretreatment. Conclusion: IPoC may offer neuroprotective outcomes in I/R insult by modulation of ARmediated pathway. result: Animals with I/R exhibited reduced motor function, and memory along with raised cerebral oxidative damage, AChE activity, inflammation, infarction, and histopathological alterations. IPoC after ischemic events recuperated the damaging outcomes of I/R insult. 60 min prior to cerebral ischemia, pretreatment with testosterone mimicked the neurodefensive outcomes of IPoC. Although, neuroprotection developed by IPoC was ended by the flutamide (ARs antagonist) pretreatment.
The role of catechins in diabetes and its complications Naturally Occurring Chemicals for the Treatment and Management of Diabetes Mellitus and Related Disorders, 2024
Rutin in the management of diabetes mellitus Naturally Occurring Chemicals for the Treatment and Management of Diabetes Mellitus and Related Disorders, 2024
Ameliorative Effects of Selective PDE-10 Inhibitors Against Ketamine-Mediated Schizophrenic Outcomes in Mice A RUCHIKA SRIVASTAVA, PRABHAT SINGH * AJPCR (Asian J Pharm Clin Res) 19 (1), 120-127 , 2026 2026
Sodium Alginate Offers Neuroprotection via Mitigating Inflammatory and Oxidative Markers in Cadmium-exposed Experimental Albino Rats BSVS Prince, Surbhi Gupta, Prabhat Singh, Sachin Tyagi Journal of Pharmaceutical Innovation 21 , 2026 2026
Cerebroprotective potential of ceftriaxone against cadmium chloride-induced cerebral inflammatory changes and oxidative insult LS Mansi Chaudhary, Prabhat Singh *, Surbhi Gupta, Vishal Kumar Biswkarma ... Naunyn-Schmiedeberg's Archives of Pharmacology , 2026 2026
Neurodegeneration and synaptic impairments mediated by chronic light-induced circadian rhythm and molecular signaling disruption: an insight beyond vision R Dumka, P Singh, V Biswkarma, L Singh, S Wadhawan Biological Rhythm Research , 2026 2026
Therapeutic Strategies for Ovariectomy-InducedVascular Dementia: Current Insights and Future Directions PS Ankit Sharma International Journal of Environmental Sciences 11 (23s), 937-946 , 2025 2025
Current and Traditional Approaches to Polycystic Ovarian Syndrome Treatment: A Review P Munday, P Singh* Current Indian Science , 2025 2025
Exploring Nature's Pharmacy: A Comprehensive Review of Herbal Plants with Neuroprotective Properties Prajapati Dipesh and Singh Prabhat * Current Green Chemistry 12 , 2025 2025 Citations: 5
Agonism of MT1 and MT2 Receptor Mitigates Oxidative Insult, Neuroinflammation, and Cerebral Injury in Cadmium Chloride Treated Animals SG Jadaun Mani Singh, Singh Prabhat * Neurochemical Research , 2025 2025 Citations: 7
Cerebroprotective Potential of Androgen Receptors in Ischemic Postconditioning against Cerebral Ischemia/Reperfusion-Induced Neurodegenerative Changes S Prabhat, G Surbhi, B Sharma, L Singh, R Bansal, M Gupta Central nervous system agents in medicinal chemistry 26 (2), 183-200 , 2025 2025 Citations: 3
Exploring the Antidiabetic, Lipid-Lowering, and Tissue Repair Benefits of Multi-Component Microspheres in Streptozotocin-Induced Diabetic Rats GP Chauhan, A. , Gupta, S. , Singh, P. , Mishra Asian Journal of Pharmaceutical and Clinical Research 18 (5), 91-95. , 2025 2025
Therapeutic applications of rutin for diabetes mellitus and its complications AS Prabhat Singh, Surbhi Gupta, Charu Bharti Natural Therapeutics for Diabetes Mellitus: Medicinal Plants, Phytochemicals … , 2025 2025
Protective Effect of Cilostazol Against Ketamine-Induced Biochemical and Behavioral Phenotype of Schizophrenia in Mice A RUCHIKA SRIVASTAVA, PRABHAT SINGH * Asian Journal of Pharmaceutical and Clinical Research 18 (4), 230–237 , 2025 2025
Implications of Glutamate Transporter-1 in Neurodegeneration-Associated Cognitive Impairments M Chaudhary, S Prabhat *, P Soni, L Singh, RK Pandey, S Gupta African Journal of Biological Sciences 6 (10), 7169-7181 , 2024 2024
Calcium Channels: A Potential Therapeutic Target for Schizophrenia A Ruchika Srivastava, Prabhat Singh* African Journal of Biological Sciences 6 (10), 6964-6974 , 2024 2024
A comprehensive review on molecular mechanism involved in arsenic trioxide mediated cerebral neurodegenerative and infectious diseases V Negi, P Singh, L Singh, RK Pandey, S Kumar Infectious Disorders-Drug TargetsDisorders) 24 (3), 51-59 , 2024 2024 Citations: 7
The Role of Catechins in Diabetes and Its Complications BS Surbhi Gupta1, Prabhat Singh2, Vaishali Negi2 Naturally Occurring Chemicals for the Treatment and Management of Diabetes … , 2024 2024
Rutin in the Management of Diabetes Mellitus CB Prabhat Singh3, Jyoti1, Meena Devi1, Nisham Rani1, Ritu Kataria1, Dev Raj ... Naturally Occurring Chemicals for the Treatment and Management of Diabetes … , 2024 2024
Montelukast ameliorates 2K1C-hypertension induced endothelial dysfunction and associated vascular dementia S Gupta, P Singh, B Sharma Current Hypertension Reviews 20 (1), 23-35 , 2024 2024 Citations: 7
Neuroprotective Effect of Morinda citrifolia on Behavioural and Biochemical Deficits in PTZ-induced Kindled Mice M Ali, P Singh, L Singh, RK Pandey, P Soni, A Singh Infectious Disorders-Drug TargetsDisorders) 23 (8), 30-42 , 2023 2023 Citations: 9
Vitamin-D attenuates chronic unpredictable mild stress induced depression like behavior in rats Priyadarshini Soni, Prabhat Singh, Lubhan Singh, Moazzam Ali, Akansha Singh European Chemical Bulletin 12 (9), 2384-2398 , 2023 2023
MOST CITED SCHOLAR PUBLICATIONS
Neuroprotective effects of agomelatine and vinpocetine against chronic cerebral hypoperfusion induced vascular dementia S Gupta, P Singh, B Mohan Sharma, B Sharma Current Neurovascular Research 12 (3), 240-252 , 2015 2015 Citations: 77
Melatonin receptor and KATP channel modulation in experimental vascular dementia P Singh, S Gupta, B Sharma Physiology & behavior 142, 66-78 , 2015 2015 Citations: 47
Modulation of transient receptor potential vanilloid subtype 1 (TRPV1) and norepinephrine transporters (NET) protect against oxidative stress, cellular injury, and vascular … S Gupta, B Sharma, P Singh, B Mohan Sharma Current neurovascular research 11 (2), 94-106 , 2014 2014 Citations: 45
Neuroprotective effects of nicorandil in chronic cerebral hypoperfusion-induced vascular dementia S Gupta, P Singh, B Sharma Journal of Stroke and Cerebrovascular Diseases 25 (11), 2717-2728 , 2016 2016 Citations: 28
Reversal in cognition impairments, cholinergic dysfunction, and cerebral oxidative stress through the modulation of ryanodine receptors (RyRs) and cysteinyl leukotriene-1 … P Singh, B Sharma Current neurovascular research 13 (1), 10-21 , 2016 2016 Citations: 26
Selective serotonin-norepinephrine re-uptake inhibition limits renovas-cular-hypertension induced cognitive impairment, endothelial dysfunction, and oxidative stress injury P Singh, B Sharma Current Neurovascular Research 13 (2), 135-146 , 2016 2016 Citations: 25
In vivo and in vitro attenuation of naloxone-precipitated experimental opioid withdrawal syndrome by insulin and selective K ATP channel modulator P Singh, B Sharma, S Gupta, BM Sharma Psychopharmacology 232 (2), 465-475 , 2015 2015 Citations: 22
Plant bioactive compounds and their mechanistic approaches in the treatment of diabetes: a review Anshika, RK Pandey, L Singh, S Kumar, P Singh, M Pathak, S Jain Future Journal of Pharmaceutical Sciences 8 (1), 52 , 2022 2022 Citations: 20
Antagonism of endothelin (ETA and ETB) receptors during renovascular hypertension-induced vascular dementia improves cognition. P Singh, S Gupta, B Sharma Current neurovascular research 13 (3), 219-229 , 2016 2016 Citations: 15
Agonism of histaminergic-H1 receptors in ischemic postconditioning during cerebral ischemia-reperfusion injury is protective. P Singh, B Sharma Current Neurovascular Research , 2020 2020 Citations: 14
Antiepileptic activity of aqueous extract of Tricosanthes dioica Roxb SG Prabhat Singh, Vipin K Garg, Pramod K Sharma Asian Journal of Plant Science and Research 2 (1), 45-47 , 2012 2012 Citations: 12
Neuroprotective Effect of Morinda citrifolia on Behavioural and Biochemical Deficits in PTZ-induced Kindled Mice M Ali, P Singh, L Singh, RK Pandey, P Soni, A Singh Infectious Disorders-Drug TargetsDisorders) 23 (8), 30-42 , 2023 2023 Citations: 9
Phytochemical constituents and pharmacological activities, profile of Morinda citrifolia: A review M Ali, P Singh, L Singh, S Kumar World J. Pharm. Res 9, 421-433 , 2020 2020 Citations: 9
Agonism of MT1 and MT2 Receptor Mitigates Oxidative Insult, Neuroinflammation, and Cerebral Injury in Cadmium Chloride Treated Animals SG Jadaun Mani Singh, Singh Prabhat * Neurochemical Research , 2025 2025 Citations: 7
A comprehensive review on molecular mechanism involved in arsenic trioxide mediated cerebral neurodegenerative and infectious diseases V Negi, P Singh, L Singh, RK Pandey, S Kumar Infectious Disorders-Drug TargetsDisorders) 24 (3), 51-59 , 2024 2024 Citations: 7
Montelukast ameliorates 2K1C-hypertension induced endothelial dysfunction and associated vascular dementia S Gupta, P Singh, B Sharma Current Hypertension Reviews 20 (1), 23-35 , 2024 2024 Citations: 7
Exploring Nature's Pharmacy: A Comprehensive Review of Herbal Plants with Neuroprotective Properties Prajapati Dipesh and Singh Prabhat * Current Green Chemistry 12 , 2025 2025 Citations: 5
Cerebroprotective Potential of Androgen Receptors in Ischemic Postconditioning against Cerebral Ischemia/Reperfusion-Induced Neurodegenerative Changes S Prabhat, G Surbhi, B Sharma, L Singh, R Bansal, M Gupta Central nervous system agents in medicinal chemistry 26 (2), 183-200 , 2025 2025 Citations: 3
Pharmacological connection of Histamine-1 (H1) Receptor Mediated Neuroprotective mechanism of Ischemic preconditioning in rat P Singh, B Sharma Research Journal of Pharmacy and Technology 14 (5), 2717-2722 , 2021 2021 Citations: 3
Review of Experimental Models of Schizophrenia RAB Bushra Naz,Prabhat Singh, Simon Nyarko International Neuropsychiatric Disease Journal 19 (1), 1 - 19 , 2023 2023 Citations: 2