Euphorbia tirucalli Latex Ingestion Modifies Heart Function and Increases Myocyte Levels of Oxidative Stress in Normotensive Rats Maria Eduarda De Souza Barroso, Edgar Hell Kampke, Rafaela Aires, Silas Nascimento Ronchi, Antonio Ferreira de Melo, Luciana Polaco Covre, Bianca Prandi Campagnaro, Ricardo Machado Kuster, Silvana Santos Meyrelles International Journal of Molecular Sciences, 2026 Euphorbia tirucalli, commonly known as Aveloz, is widely used in Brazilian folk medicine for its purported antibacterial, antiviral, and antitumoral properties. However, scientific evidence regarding its systemic in vivo effects, particularly on the cardiovascular system, remains limited. This study investigated the impact of oral E. tirucalli latex ingestion on cardiac hemodynamics and associated molecular alterations in normotensive Wistar rats. Animals received water (control) or E. tirucalli latex (13.47 mg/kg) by oral gavage for 15 days. Hemodynamic parameters were assessed through noninvasive blood pressure monitoring and direct measurements of left ventricular systolic (LVSP) and end-diastolic pressures (LVEDP), cardiac cycle duration, rates of pressure development (dP/dTmax and dP/dTmin), and the left ventricular relaxation constant (Tau). Oxidative stress and inflammation were evaluated by plasma advanced oxidation protein products (AOPP) and myeloperoxidase (MPO), respectively, while reactive oxygen species production and apoptosis were analyzed in isolated cardiomyocytes. Although systemic blood pressure remained unchanged, E. tirucalli increased LVSP, LVEDP, cardiac cycle duration, and dP/dTmax, while reducing Tau. These alterations were accompanied by elevated AOPP and MPO levels, increased cardiomyocyte hydrogen peroxide, and higher rates of early apoptosis, indicating that E. tirucalli latex alters cardiac hemodynamics and promotes oxidative and inflammatory cardiac injury.
The Association Between Metabolomic and Usual Biochemical Data Helps to Detect Insulin Resistance Fábio S. Pimenta, Camila Conde, Radael R. Rodrigues Júnior, Bianca P. Campagnaro, Thiago M. C. Pereira, Manuel Campos-Toimil, Silvana S. Meyrelles, Elisardo C. Vasquez Biomedicines, 2026 Background: Chronic noncommunicable diseases account for nearly 80% of global deaths and are strongly associated with insulin resistance (IR). One of the most significant clinical findings of the past two decades is that the molecular mechanisms underlying immune and metabolic systems have been evolutionarily conserved across species. Methods: This study included 34 volunteers (19 men and 15 women). Demographic data were collected using validated questionnaires. Anthropometric measurements (weight, height, waist-to-hip ratio, and body composition assessed by tetrapolar bioimpedance) were obtained directly. Laboratory analyses included fasting glucose and insulin, glycated hemoglobin, HDL cholesterol, total cholesterol, triglycerides, organic aciduria, and additional biochemical markers assessed using standard methods. Group comparisons were performed using parametric or nonparametric statistical tests according to data distribution, as specified in the figure legends. Results: The primary analyses focused on identifying early metabolomic alterations associated with insulin resistance in individuals whose conventional biochemical parameters were within laboratory reference ranges. Individuals with a TG/HDL ratio > 2 and increased urinary kynurenate excretion exhibited a 3.6-fold higher relative risk of insulin resistance, while elevated insulin levels combined with urinary α-ketoisovalerate were associated with a 2.7-fold increased risk. Significant differences in plasma insulin, HbA1c, and HOMA-IR were observed between healthy and diseased individuals (p < 0.05), indicating early metabolic dysfunction preceding clinical disease onset. Conclusions: Metabolomic biomarkers serve as reliable indicators of subclinical metabolic disturbances, revealing significant risks in major metabolic pathways even in individuals with conventional exams within normal limits. Early detection through these metabolomic markers may enable personalized interventions aimed at preserving cellular function and systemic metabolic balance.
Short-Term Intake of Euphorbia tirucalli Latex Modifies Kidney Function in Rats: Possible Role of Oxidative Stress and Inflammatory Response Edgar Kampke, Maria Barroso, Leonardo Escouto, Luciana Covre, Ágata Gava, Bianca Campagnaro, Ricardo Kuster, Silvana Meyrelles Antioxidants, 2025 Medicinal plants have been traditionally used for generations, often without scientific validation. Euphorbia tirucalli (E. tirucalli), a plant native to Africa, is commonly employed in folk medicine for treating various ailments, including cancer. However, most studies involving this species are limited to in vitro models, and its systemic effects remain poorly understood. This study aimed to evaluate the impact of E. tirucalli latex on renal function in healthy Wistar rats. Animals were divided into two groups: a control group receiving water and a treated group receiving E. tirucalli latex (13.47 mg/kg) by gavage for 15 days. Renal function was assessed by measuring glomerular filtration rate (GFR), renal plasma flow (RPF), renal blood flow (RBF), renal vascular resistance (RVR), and mean arterial pressure (MAP). Additionally, oxidative stress markers, reactive oxygen/nitrogen species, and inflammatory activity were analyzed in renal tissue. E. tirucalli significantly reduced GFR, RPF, and RBF, while increasing RVR and MAP. Renal tissue exhibited elevated levels of advanced oxidation protein products, myeloperoxidase, nitric oxide, and peroxynitrite/hydroxyl radicals. These findings indicate that E. tirucalli latex adversely affects renal hemodynamics and promotes oxidative and inflammatory damage, suggesting potential nephrotoxic effects, even in healthy subjects.
Infrared laser therapy decreases systemic oxidative stress and inflammation in hypercholesterolemic mice with periodontitis Isadora Martins Ribeiro, Maria Eduarda de Souza Barroso, Edgar Hell Kampke, Larissa Trarbach Figueiredo Braga, Bianca Prandi Campagnaro, Silvana dos Santos Meyrelles Lipids in Health and Disease, 2023 Background Near-infrared irradiation photobiomodulation (NIR-PBM) has been successfully used in periodontal treatment as an adjuvant tool to locally improve cell function and regeneration. Although the relationship between periodontitis and systemic disease constitutes an important aspect of periodontal clinical research, the systemic effects of NIR-PBM in periodontitis are not well known. In this study, we aimed to investigate the effects of NIR-PBM on systemic oxidative stress and inflammation in an apolipoprotein E (ApoE) knockout mouse model of periodontal disease (PD). Methods We evaluated alveolar bone loss by measuring the distance from the cementoenamel junction (CEJ) to the alveolar bone crest (ABC), reactive oxygen species (ROS) production in blood cells, inflammatory activity, plasma cholesterol levels, and lipid peroxidation levels in three experimental groups: (1) ApoEC, control group without intervention; (2) ApoEP, first molar ligation-induced periodontitis for 4 weeks; and (3) ApoEP + PBM, exposed to 808 nm continuous wave, ø ~ 3 mm2, 100 mW, 60 s of NIR-PBM for 7 consecutive days after 4 weeks of periodontitis. At the end of the experimental protocols, ApoEP mice presented significantly increased alveolar bone loss, ROS production, inflammatory activity, plasma cholesterol, and lipid peroxidation levels compared to the ApoEC group (P < 0.05). NIR-PBM for 7 days in the ApoEP + PBM mice significantly decreased systemic ROS production, inflammatory response, plasma cholesterol, and lipid peroxidation levels, similar to those found in the ApoEC group (P > 0.05). However, it was not capable of preventing alveolar bone loss (P > 0.05 compared to ApoEP mice). Conclusion A 7-day treatment with NIR-PBM effectively reduces systemic oxidative stress and inflammatory parameters in hypercholesterolemic mice with PD. However, more studies with longer evaluation times are needed to confirm the systemic effects of locally applied NIR-PBM on PD associated with hypercholesterolemia.
Unravelling the Gastroprotective Potential of Kefir: Exploring Antioxidant Effects in Preventing Gastric Ulcers Larissa Zambom Côco, Rafaela Aires, Glaucimeire Rocha Carvalho, Eduarda de Souza Belisário, Michelle Khai Khun Yap, Fernanda Gobbi Amorim, Javier Conde-Aranda, Breno Valentim Nogueira, Elisardo Corral Vasquez, Thiago de Melo Costa Pereira, Bianca Prandi Campagnaro Cells, 2023 The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-α levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1–35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-α systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.
Development and Evaluation of Virola oleifera Formulation for Cutaneous Wound Healing Glaucimeire R. Carvalho, Débora S. Braz, Talita C. O. Gonçalves, Rafaela Aires, Larissa Z. Côco, Marcio Guidoni, Marcio Fronza, Denise C. Endringer, Antonio D. S. Júnior, Manuel Campos-Toimil, Breno V. Nogueira, Elisardo C. Vasquez, Bianca P. Campagnaro, Thiago M. C. Pereira Antioxidants, 2022 In regions adjacent to the Brazilian Atlantic Forest, Virola oleifera (VO) resin extract has been popularly used for decades as a skin and mucosal healing agent. However, this antioxidant-rich resin has not yet been investigated in wound healing, whose physiological process might also be aggravated by oxidative stress-related diseases (e.g., hypertension/diabetes). Our aim, therefore, was to investigate whether VO resin presents healing effects through an innovative cream for topical applications. For this, adult male Wistar rats were divided into four groups. Then, four 15 mm excisions were performed on the shaved skin. All treatments were applied topically to the wound area daily. At the end of experiments (0, 3rd, and 10th days) macroscopic analysis of wound tissue contraction and histological analysis of inflammatory cell parameters were performed. The group treated with VO cream showed the best wound contraction (15%, p < 0.05) and reduced levels of lipid peroxidation and protein oxidation (118% and 110%, p < 0.05, respectively) compared to the control group. Our results demonstrated the healing capacity of a new formulation prepared with VO, which could be, at least in part, justified by antioxidant mechanisms that contribute to re-epithelialization, becoming a promising dermo-cosmetic for the treatment of wound healing.
The Gut Microbiota-Brain Axis: A New Frontier on Neuropsychiatric Disorders Sarha A. L. Queiroz, Alyne M. M. Ton, Thiago M. C. Pereira, Bianca P. Campagnaro, Larissa Martinelli, Aitor Picos, Manuel Campos-Toimil, Elisardo C. Vasquez Frontiers in Psychiatry, 2022 Alzheimer's disease (AD) is a progressive and incurable neurodegenerative disorder of integrative areas of the brain, characterized by cognitive decline and disability resulting in negative impacts on the family of the patients and the health care services worldwide. AD involves oxidative stress, neuroinflammation and accelerated apoptosis, accompanied by deposition of amyloid-β peptide plaques and tau protein-based neurofibrillary tangles in the central nervous system. Among the multiple factors that contribute to the onset and evolution of this disease, aging stands out. That is why the prevalence of this disease has increased due to the constant increase in life expectancy. In the hope of finding new, more effective methods to slow the progression of this disease, over the last two decades, researchers have promoted “omics”-based approaches that include the gut microbiota and their reciprocal interactions with different targets in the body. This scientific advance has also led to a better understanding of brain compartments and the mechanisms that affect the integrity of the blood-brain barrier. This review aims to discuss recent advances related to the gut-brain-microbiota axis in AD. Furthermore, considering that AD involves psychiatric symptoms, this review also focuses on the psychiatric factors that interact with this axis (an issue that has not yet been sufficiently addressed in the literature).
Protective effects of kefir in the angiotensin II-dependent hypertension Brenna L. Monteiro, Ananda T. Dias, Sthephany C. Wanderkoke, Rodrigo Yokota, Dulce E. Casarini, Marcos A.S. Leal, Breno V. Nogueira, Silvana S. Meyrelles, Manuel Campos-Toimil, Bianca P. Campagnaro, Ruy R. Campos, Thiago M.C. Pereira, Elisardo C. Vasquez Journal of Functional Foods, 2020
