Comparative analysis of conventional and IRMS techniques for honey adulteration detection in accordance with ISIRI standards Abbas Alimoradian, Nafiseh Khansari, Mahdi Abdollahi, Behnoosh Ansari Asl, Sonia Asadi, Akbar Yousefi Maghool, Arash Rabiei, Ali Khademi, Mohsen Shamsi BMC Research Notes, 2025 This study assesses the comparative performance of Isotope Ratio Mass Spectrometry (IRMS) and conventional techniques in detecting honey adulteration. Standard analytical methods, in accordance with ISIRI guidelines, were evaluated alongside δ13C isotopic analysis via IRMS across a dataset of 20 honey samples. Conventional analytical techniques were employed to evaluate parameters such as pre-hydrolysis reducing sugars, sucrose content, the fructose-to-glucose ratio, proline, and hydroxymethylfurfural (HMF). The advanced IRMS technique was utilized to determine δ13C values for glucose with high accuracy and sensitivity. The carbohydrate profile of honey, including glucose, fructose, disaccharides, and trisaccharides, was analyzed using LC/IRMS. A comparative assessment of both methods was conducted on twenty honey samples, following ISIRI guidelines. While conventional methods met ISIRI criteria in 18 out of 20 samples, IRMS produced contrasting findings—only 2 samples were identified as genuine and free from adulteration. Statistical evaluation using Prism software revealed a significant discrepancy between the two techniques, with a p-value < 0.05, indicating stronger confidence in IRMS results. The findings highlight the necessity of reconsidering current protocols for honey adulteration detection in order to preserve the purity and credibility of this high-value natural product.
Processed and Unprocessed Honey: A Comparative Study on the Effect of Honey on Blood Sugar Levels in Diabetics Abass Alimoradian, Elham Mohajerani, Atefeh Navabi, Bahman Sadeghi Sade, Mohaddeseh Asafari Journal of Kerman University of Medical Sciences, 2025 Background: Honey is a natural sweetener that has been used for hundreds of years for its nutritional and medicinal properties, but the impact of unprocessed versus processed honey on blood glucose levels in diabetics is subject to debate. This study aimed to assess the impact of processed and unprocessed honey on blood sugar levels in diabetics. Methods: This randomized, double-masked clinical trial included 90 diabetic patients. The patients consumed 30 g of both unprocessed and processed honey, and blood samples were collected before and after the consumption of each honey type to measure the blood glucose levels. Laboratory factors of honey, such as sugar before hydrolysis, proline, and fructose to glucose ratio (F/G), hydroxymethylfurfural (HMF), and sucrose content, were also analyzed. Results: The results showed that unprocessed honey had a more substantial effect on blood glucose levels compared to processed honey, but the difference was not significant (P<0.07). The glucose levels were 241.2±72.4 after ingesting unprocessed honey and 197.7±53.3 after ingesting processed honey. There was no correlation between the laboratory factors of honey and the blood glucose levels. Conclusion: Unprocessed honey had a more detrimental impact on blood glucose levels in diabetics than processed honey, and the laboratory factors of honey did not have a significant impact on the glycemic reaction. Consequently, diabetics have to abstain from eating unprocessed honey and restrict their intake of processed honey. The implications and mechanisms involved in the effect of honey on blood sugar levels require further research.
Hepatoprotective and hypolipidemic effects of Hibiscus gossypifolius, Trachyspermum copticum, Taraxacum officinale, and Rosmarinus officinalis extracts in a rat model of diet-induced hypercholesterolemia Journal of Medicinal Plants, 2025
Testosterone signaling pathways for reducing neuropathic pain in a rat model of spinothalamic tract lesion Abbas Alimoradian, Fatemeh Abbaszadeh, M. Jorjani Iranian Journal of Basic Medical Sciences, 2024 Objective(s): Most individuals who suffer from spinal cord injury (SCI) experience neuropathic pain, which currently has no effective treatment. In this study, we examined how testosterone affects neuropathic pain resulting from SCI. Materials and Methods: We administered three different doses of testosterone (4, 8, 16 mg/kg, intraperitoneal) to male rats after an electrolytic lesion of the spinothalamic tract. We then conducted behavioral tests, including open field and von Frey tests, within 28 days post-SCI. On day 28 after SCI, we analyzed spinal tissue using western blot to measure the levels of ionized calcium binding adaptor molecule 1 (Iba1), glial fibrillary acidic protein (GFAP), phospho-extracellular signal-regulated kinase (p-ERK1/2), and p-P38 at the injury site. Results: The results showed that testosterone significantly improved both motor activity and mechanical allodynia compared to the SCI-only group. Testosterone also inhibited microglia and astrocyte activation. Furthermore, testosterone significantly decreased p-P38 and p-ERK levels. Conclusion: The findings indicate that testosterone may alleviate SCI-induced neuropathic pain by inhibiting the activation of astrocytes and microglia, as well as suppressing MAPK signaling pathways.
Hydroalcoholic extract of Scrophularia striata has a significant therapeutic effect on thioacetamide-induced liver cirrhosis in rats Jamal Amri, Mohammad Jamalian, Zahre Salami, Ahmad Akbari, Mona Alaee, Fatemeh Azizi Soleiman, Abbas Alimoradian Hormone Molecular Biology and Clinical Investigation, 2023 ObjectivesLiver cirrhosis is one of the most important causes of death from liver diseases. Nowadays, the use of herbal medicines has increased due to its availability, less side effects and cheapness for the treatment of liver diseases. The present study was conducted to examine therapeutic effects of hydroalcoholic extract ofScrophularia striata(S. striata) on thioacetamide-induced liver cirrhosis in rats through evaluate its effects on oxidative stress markers and the expression of metalloproteinase 1 (TIMP 1), toll-like receptor-4 (TLR-4), and Mitofusin (MFN2) genes.Methods24 male rats were selected by simple random sampling. Rats were randomly assigned to four groups: group I: healthy rats, group II: thioacetamide (TAA) injected rats, group III: TAA injected rats+100 mg/kg bw ofS. striataand group IV: TAA injected rats+200 mg/kg bw ofS. striata. Liver cirrhosis was induced in rats by a 300 mg/kg bw TAA administration twice with an interval of 24 h. After 8 weeks of treatment byS. striataat doses of 100 and 200 mg/kg bw, biochemical factors and oxidative stress markers (SOD, TAC, GPX, CAT and MDA) were measured using spectrophotometric methods. Also, gene expression of TIMP 1, TLR-4, and MFN2 were analyzed using real-time PCR. ANOVA and Bonferroni post hoc test analysis were applied to evaluate the data.ResultsThe results showed theS. striataextract significantly improve the serum ALT, AST and ALP levels, TIMP 1, TLR-4, and MFN2 genes and oxidative stress markers (SOD, TAC, GPX, CAT and MDA) in the liver tissues when compared to control group (p<0.05). Also, it was found that the beneficial effects of theS. striatawere dose-dependent.ConclusionsBased on the results obtainedS. striataby reducing the expression of TIMP 1, TLR-4, and MFN2 genes and improving oxidative stress might be used as adjuvant treatment for liver cirrhosis.
Protective effects of paraoxonase-1, vitamin E and selenium, and oxidative stress index on the susceptibility of low density lipoprotein to oxidation in diabetic patients with/without coronary artery disease Fatemeh Mehvari, Fatemeh Imanparast, Pegah Mohaghegh, Abbas Alimoradian, Nafiseh Khansari, Behnoosh Ansari Asl, Ali Khosrowbeygi European Journal of Medical Research, 2023 Background The oxidative modification of low density lipoprotein (LDL) is closely associated with an increased risk for coronary artery disease (CAD) in diabetic patients. The purpose of this study is to investigate the relation between serum vitamin E and selenium, paraoxonase-1 (PON1) activity, total antioxidant capacity (TAC), total oxidant status (TOS), malondialdehyde (MDA), and oxidative stress index (OSI) values with the susceptibility of LDL to oxidative modification and the possibility of CAD in diabetic patients. Method This study was designed as a case control survey of 82 diabetes patients divided into two groups including T2DM alone (as group I) and both T2DM and CAD (as group II). Fasting blood samples were taken to the assay of fasting blood glucose (FBG), HbA1c, total cholesterol (TC), TAC, TOS, MDA, OSI, vitamin E, selenium, oxidized low density lipoprotein (Ox-LDL), and activity of PON1. Results Ox-LDL, MDA, TOS, and OSI values in groups II were significantly higher compared with group I (all with P value = 0.000). TAC, vitamin E, selenium, and PON1 activity values were significantly lower in group II compared with groups I (P value = 0.000; P value = 0.000; P value = 0.007; P value = 0.003, respectively). There were significant relationships between the amounts of TAC, TOS, OSI, and vitamin E with the amounts of PON1 activity and Ox-LDL (p < 0.05). But Ox-LDL and PON1 activity correlated weakly with together (p = 0.094). Conclusion Results of this study support the belief that oxidative stress might be an important etiologic factor which makes some diabetics more susceptible to CAD. Increased oxidative stress may be a potential therapeutic target in the prevention and management of CAD in diabetic patients.
Evaluation of the Synergistic Effect of LL-37 and Oncorhyncin II Recombinant Proteins on Staphylococcus aureus Under In Vitro Conditions Mozhdeh Safari, Robab Rafiei Tabatabaei, Hamid Abtahi, Abbas Alimoradian, Shohreh Fahimirad Jundishapur Journal of Microbiology, 2023 Background: The treatment of Staphylococcus aureus infections has become a public health crisis due to the extensive development of antimicrobial resistance. Antimicrobial peptides (AMPs) have been introduced as promising naturally-derived antimicrobial alternatives to antibiotics. LL-37 and oncorhyncin II are 2 AMPs with notable proven antibacterial effects. Objectives: This study aimed to produce recombinant LL-37 and oncorhyncin II and investigate their synergistic effects on S. aureus (ATCC25923). Methods: The synthetic genes of LL-37 and oncorhyncin II were individually ligated into the pET32a expression vector. Transformed pET32a was introduced into Escherichia coli BL21 as an expression host. The protein expression and purification steps were optimized, and the biological effectiveness of the peptides was evaluated by assessing the minimum inhibitory concentration (MIC), time-kill, and growth kinetic tests against S. aureus. Results: The MIC assay confirmed the effective antibacterial performances of LL-37 and oncorhyncin II against S. aureus at 30.6 and 47.93 µg/mL, respectively. The peptides’ synergistic activity was validated by the checkerboard method. A combination of LL-37 and oncorhyncin II at 2 × MIC showed a sharp decline of the viable cells with over 3-time reductions in log 10 colony-forming units (CFU)/mL within the first 5 hours. The growth kinetic results confirmed the high effectiveness of the peptides’ combination in eliminating the bacterial inoculum turbidity by 50% reduction during the first hour of exposure. Conclusions: The produced recombinant LL-37 and oncorhyncin II showed effective antimicrobial function against S. aureus. The synergistic performance of the peptides was repeatedly confirmed through checkerboard, time-kill, and growth kinetic assays.
Effect of Silymarin on Hepatic Complications Caused by Methotrexate and its Analgesic Effects in Patients with Rheumatoid Arthritis Scientific Journal of Kurdistan University of Medical Sciences, 2023
Assessment of complications caused by the prescription-free consumption of herbal medicine with the purpose of weight gain: A case report Traditional and Integrative Medicine, 2021
The effects of hydro-alcoholic extracts of allium sativum L. and orchismaculata L. on spermatogenesis index and testosterone level in cyclophosphamide-treated rats Journal of Kerman University of Medical Sciences, 2020
The effect of streptozotocin-induced chronic hyperglycemia on respiratory effects of morphine in rats Journal of Babol University of Medical Sciences, 2020
Investigation of pulmonary fungal infections in immunocompromised patients Tehran University Medical Journal, 2019
Protective effects of omega-3, atorvastatin, vitamin E and vitamin C against doxorubicin-induced cardiotoxicity in rats: A comparison study Physiology and Pharmacology Iran, 2018
Investigation of the effect of Ginkgo biloba leaf extract on spatial memory impairment and hippocampal neuronal loss caused by diabetes induced by streptozotocin in rats Abbas Alimoradian, Department of Pharmacology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran., Saeed Ghasemi, Faculty of Paramedicine, Arak University of Medical Sciences, Arak, Iran., Mohammad Zahiri, Faculty of Paramedicine, Arak University of Medical Sciences, Arak, Iran., Amir Hossein Saeedi, Faculty of Paramedicine, Arak University of Medical Sciences, Arak, Iran., Hossein Miladi, Department of Pathology, Imam Khomeini Hospital, Arak, Iran., Mehdi Sadegh, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran Scientific Journal of Kurdistan University of Medical Sciences, 2018
Protective effects of pomegranate juice on nephrotoxicity induced by captopril and gentamicin in rats Iranian Journal of Kidney Diseases, 2017
Relationship of dopamine of the nucleus accumbens with intra-infralimbic apomorphine microinjection Iranian Journal of Basic Medical Sciences, 2013
