Rina Hidayati Pratiwi

@unindra.ac.id

Biological Education; Mathematics and Natural Sciences Education
Universitas indraprasta pgri

Rina Hidayati Pratiwi


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https://researchid.co/rinahp2024

RESEARCH, TEACHING, or OTHER INTERESTS

Microbiology, Microbiology (medical), Biotechnology, Drug Discovery
8

Scopus Publications

956

Scholar Citations

15

Scholar h-index

19

Scholar i10-index

Scopus Publications

  • Disruption of hyphal development in Candida albicans ATCC 10231 by bioactive compound from Pseudomonas azotoformans UICC B-91
    Yonita Aprilia Putri, Rina Hidayati Pratiwi, Wibowo Mangunwardoyo
    Journal of Applied Pharmaceutical Science, 2024
    Candidiasis and other fungal infections are becoming more frequent. The search for appropriate antibiotics for fungal infections is still ongoing. Pseudomonas azotoformans University of Indonesia Culture Collection (UICC) B-91 has potential as an anticandidal. The purpose of this study was to determine the mechanism of inhibition of the metabolite of P. azotoformans UICC B-91 from the medium against morphologically pathogenic fungi. The results showed metabolite compound P. azotoformans UICC B-91 at a concentration of 100 and 80 mg/ml had strong inhibitory power, and 60 mg/ml had moderate inhibition. Determination of the minimum inhibitory concentration value of the medium solution of P. azotoformans UICC B-91 against Candida albicans American Type Culture Collection (ATCC
  • Antifungal effect of Pseudomonas azotoformans UICC B-91 metabolites on hyphal growth of Trichophyton mentagrophytes
    R H Pratiwi, Y A Putri, W Mangunwardoyo, S Sufiandi
    Iop Conference Series Earth and Environmental Science, 2023
    Pseudomonas azotoformans UICC B-91 is known to have antimicrobial potential. The purpose of this study was to determine the inhibition mechanism of the metabolites of P. azotoformans UICC B-91 on the activity of Trichophyton mentagrophytes through morphological observations. The results showed that the metabolites produced by P. azotoformans UICC B-91 were inhibited growth of T. mentagrophytes. The zone of inhibition produced by the extract solution of P. azotoformans UICC B-91 against T. mentagrophytes at concentrations of 100 mg/mL and 80 mg/mL showed strong inhibitory power, with average values of 17.82 ± 0.32 mm and 10.79 ± 0.74 mm, respectively. The microscopic morphology of T. mentagrophytes after treatment with 0.1 g of ethyl acetate extract of P. azotoformans UICC B-91 showed wider hyphae compared to the control hyphae; abnormal cytoplasm; and granular structures between the hyphae, attached to the lateral side of the hyphae, and between the cell wall and the cell membrane. The mechanism of inhibition of the metabolites from the ethyl acetate extract of P. azotoformans UICC B-91 against T. mentagrophytes is thought to involve interference with cell walls and the function of the cell membrane.
  • Antimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae)
    Rina Hidayati Pratiwi, Eva Oktarina, Wibowo Mangunwardoyo, Iman Hidayat, Endang Saepudin
    Pharmacognosy Journal, 2022
    P. azotoformans strain UICC B-91 was isolated from Nessia altissima Blume, traditional medicinal plant endemic from Indonesia1 that effective against gonorrhoeae2 due to Neisseria gonorrhoeae infection. But there is no publication research on bioactive natural compound from N. altissma, yet. Endophytic microorganism has genetic diversity due to adaption to host environment and might be transfer gen from host, that relate to the bioactive compound produced.3 Previous research has been observed the antimicrobial activity from centrifugated extract of endophytic P. azotoformans, against several bacteria and C. albicans.4 with notice result on its anticandidal activity. For further observation, the antimicrobial compounds were identified by mass spectrophotometry.
  • In vitro antimicrobial activities of several extracts endophytic Pseudomonas azotoformans UICC B-91
    E Oktarina, R H Pratiwi, W Mangunwardoyo, I Hidayat, E Saepudin
    Iop Conference Series Earth and Environmental Science, 2021
    Pseudomonas azotoformans known for the benefit on agriculture area as soil fertility enhancer. Pseudomonas azotoformans UICC B-91 was successfully isolated from tissues of N. altissima which traditionally known for treating infectious diseases. Therefore, endophytic P. azotoformans might contain antimicrobial compound. This research aims on exploring the antimicrobial activities of several extracts P. azotoformans UICC B-91 against pathogenic microbes. Isolates were extracted with dichloromethane, chloroform, and ethyl acetate. Each crude extract was observed for its potential antimicrobial activity with disc diffusion method against several pathogen microbes. Bioactive compound was assayed by thin layer chromatography (TLC) and screened by bioautography. Results indicated that all crude extract have inhibitory effect on Escherichia coli ATCC 8739, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 6583, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 25241, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659 and Candida albicans ATCC 10231. Highest clear zone was on dichloromethane extract. TLC fraction of dichloromethane extract was able to inhibit growth on pathogenic bacteria (except for B. subtilis ATCC 19659 and S. aureus ATCC 6583) and fungi at minimum concentration in the range 125-1,000 μg/mL and 125 μg/mL, respectively. Bioactive compound from endophytic P. azotoformans extracted with dichloromethane have antibacterial and anticandidal effect.
  • Isolation and structure elucidation of phenazine derivative from Streptomyces sp. strain UICC B-92 isolated from neesia altissima (malvaceae)
    Rina Hidayati Pratiwi, Iman Hidayat, Muhammad Hanafi, Wibowo Mangunwardoyo
    Iranian Journal of Microbiology, 2020
    Background and Objectives: Endophytic actinomycetes have been known as a promising source for new antibiotics discov- ery against susceptible and resistant forms of pathogenic microorganisms. This study was aimed at determining antibacterial compound from Streptomyces sp. strain B-92 isolated from a medicinal plant Neesia altissima.Materials and Methods: Streptomyces sp. strain UICC B-92 was endophytic actinomycetes of N. altissima that obtained from Universitas Indonesia Culture Collection (UICC). Isolation and determination of bioactive compound were carried out using thin layer chromatography (TLC), nuclear magnetic resonance spectroscopy (NMR), and liquid chromatography mass spectrometry (LC-MS) analyses. An in vitro antibacterial assay of pure bioactive compound from the endophytic actinomycetes strain was performed against Bacillus cereus strain ATCC 10876, Escherichia coli strain ATCC 25922, Salmonella typhimurium strain ATCC 25241, Shigella flexneri strain ATCC 12022 and Staphylococcus aureus strain ATCC25923.Results: The bioactive compound was identified as 4-((3S,4R,5S)-3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H- pyran-2-yloxy) phenazine-1-carboxylic acid. In vitro antimicrobial assay showed that bioactive compound of Streptomyces sp. strain UICC B-92 exhibited antagonistic activities against two Gram-positive bacteria, viz, B. cereus strain ATCC 10876 and S. aureus strain ATCC 25923.Conclusion: The findings of this research showed that, bioactive compound of Streptomyces sp. strain UICC B-92 is sug- gested a new compound based on glycoside structure and its position.
  • Identification and screening of rare actinomycetes isolated from Neesia altissima Bl.
    R. H. Pratiwi, I. Hidayat, M. Hanafi, W. Mangunwardoyo
    Aip Conference Proceedings, 2017
    Actinomycetes is the main source of antibiotics and endophytic actinomycetes from medicinal plants has considerable potential as like the host. The aim of this research is to identify rare actinomycetes isolated from Neesia altissima and to screen their antagonistic activity against diarrhea-causing bacteria in order to find new potential secondary metabolites. Samples of N. altissima were collected from mount Halimun-Salak National Park. Endophytic actinomycetes were isolated from roots of N. altissima by surface sterilization method. Screening of antagonistic activity was conducted against five diarrhea-causing bacteria such as Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 25241, Shigella flexneri ATCC 12022, and Staphylococcus aureus ATCC 25923 by using diffusion disc methods. The endophytic actinomycete showed in vitro antibacterial activity against four diarrhea-causing bacteria, except the B. cereus ATCC 10876. The phylogenetic tree generated from 16S rRNA sequ...
  • Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima
    Rina Hidayati Pratiwi, Iman Hidayat, Muhammad Hanafi, Wibowo Mangunwardoyo
    Journal of Microbiology, 2017
    This study's aim was to determine the identity of antibacterial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds' mechanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was observed morphologically through scanning electron microscopy (SEM). This study successfully identified the (2E,5E)-phenyltetradeca-2,5-dienoate antibacterial compound (molecular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca-2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.
  • Identification and characterization of three endophytic bacteria from Neesia altissima (Malvaceae) antagonistic to diarrhea-causing bacteria
    Pratiwi, R. H., Hidayat, I., Hanafi, M., Mangunwardoyo, W.
    Malaysian Journal of Microbiology, 2016
    Aims: Diarrheal disease is one of serious healthcare problems in developing countries. Endophytic bacteria have been known as a promising source of new antibiotics against susceptible and resistant forms of microorganisms. In this study, we identified three endophytic bacteria isolated from Neesia altissima and screened their antagonistic activity against diarrhea-causing bacteria in order to find new potential secondary metabolites. Methodology and results: Samples of N. altissima were collected from mount Halimun-Salak national park. Endophytic bacteria were isolated from roots, barks, and fresh leaves of N. altissima by surface sterilized method. Screening of antagonistics activity was conducted against five diarrhea-causing bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Shigella flexneri, and Bacillus cereus using crude extract dilution and diffusion disc methods. Three endophytic bacteria showed antagonistic activity against the pathogenic bacteria. Identification of the three potential endophytic bacteria using molecular analysis showed that two isolates determined as Pseudomonas aeruginosa and one isolate belongs to P. azotoformans. Conclusion, significance and impact study: Crude extract of P. aeruginosa (strain 1.4.1A and 1.2.7D) and P. azotoformans (strain 1.8.7KB) showed growth inhibition activity to the diarrhea-causing bacteria. This is the first report of P. azotoformans exhibited antagonistic activities against diarrhea-causing bacteria. This data suggested that bacterial endophytes from N. altissima provided potential sources for the discovery of new secondary metabolites to combat the diarrhea-causing bacteria. This finding highlights potential prospects of endophytic bacteria utilization from endemic medicinal plants for the discovery of novel bioactive compounds.

RECENT SCHOLAR PUBLICATIONS

  • Examining the Dialectics of Positivism and Logical Positivism in Philosophy at the University Level
    YRY Sentausa, MT Annisa, D Sunaryati, T Anita, RH Pratiwi
    el-Nizami: Jurnal Manajemen Pendidikan 3 (1), 1-5 , 2026
    2026
  • Edukasi Pengolahan Limbah Sapi Menjadi Pupuk Organik Cair Sebagai Upaya Pengurangan Pencemaran Lingkungan di Kelurahan Harjasari, Bogor Selatan
    ZF A'ini, ZR Alfy, RH Pratiwi
    Jurnal Medika: Medika, 1477-1482 , 2026
    2026
  • EKSPLORASI JENIS DAN PEMANFAATAN TANAMAN ENDEMIK DI KECAMATAN BOGOR SELATAN
    S Noer, M Alamsyah, RH Pratiwi, M Fadhillah
    Jurnal Biogenerasi 11 (1), 116-128 , 2026
    2026
  • Pengaruh Media Pembelajaran Berbasis Teknologi dalam Pembelajaran Fisika SMA
    J Fadhilah, DE Anggraeni, J Akmaludin, M Ikhsan, M Cantika, RH Pratiwi
    JDP (JURNAL DINAMIKA PENDIDIKAN) 12 (2), 304-313 , 2026
    2026
  • Pengaruh Efikasi Diri dan Resiliensi Akademik terhadap Kemampuan Berpikir Reflektif IPA (Survei pada SMP Negeri di Kota Depok)
    D Andriani, A Suryana, RH Pratiwi
    ALFARISI: Jurnal Pendidikan MIPA 8 (3), 366-376 , 2025
    2025
    Citations: 1
  • Pengaruh Literasi Sains dan Kebiasaan Berpikir terhadap Kemampuan Pemecahan Masalah Ilmu Pengetahuan Alam
    E Muhartono, A Suryana, RH Pratiwi
    ALFARISI: Jurnal Pendidikan MIPA 8 (3), 427-436 , 2025
    2025
  • The effect of using PhET simulation media on energy and work materials on improving students' learning outcomes and critical thinking skills: A literature study
    F Purwono, A Suhendar, S Fathani, F Fadli, RH Pratiwi
    Priviet Social Sciences Journal 5 (12), 621-627 , 2025
    2025
  • Bioactivity of Pseudomonas azotoformans UICC B-91 as an agent with antimicrobial activity, Salmonella typhi and Microsporum canis
    RH Pratiwi, R Juliadhi
    Edubiotik: Jurnal Pendidikan, Biologi dan Terapan 10 (02), 375-385 , 2025
    2025
  • The impact of tourism activities on the microbiological water quality of the Cipamingkis River tourism area
    VY Fadilah, S Noer, RH Pratiwi
    Biological Environment and Pollution 5 (2), 95-105 , 2025
    2025
  • Peran Laporan Terintegrasi dalam Keberlanjutan Keuangan pada Bank Syariah
    K Karsam, S Safrudin, R Pratiwi
    Jurnal Akuntansi dan Keuangan Kontemporer (JAKK) 8 (2), 453-467 , 2025
    2025
  • Peran AI dalam Inovasi Pembelajaran MIPA di Sekolah: Peluang dan Tantangan
    WT Sejati, R Rizki, I Nirmalasari, S Sundari, RH Pratiwi
    Jurnal Penelitian, Pendidikan dan Pengajaran: JPPP 6 (3), 212-218 , 2025
    2025
  • Pengaruh Model Inquiry terhadap Hasil Belajar Peserta Didik Kelas X pada Materi Ekologi dan Keanekaragaman Hayati
    R Pratiwi, DP Supendi, A Shena, B Siregar, S Sugiantoro, RH Pratiwi
    Jurnal Penelitian, Pendidikan dan Pengajaran: JPPP 6 (3), 228-232 , 2025
    2025
  • PENERAPAN PRODUK CREAMY LEMON BIR PLETOK DARI JAHE EMPRIT (Zingiber officinale) KE MASYARAKAT SEBAGAI MINUMAN KESEHATAN
    RH Pratiwi, E Sulistyaniningsih, LN Putri, J Jepri, S Fauziah, SER Dewi, ...
    Prosiding Semnas PenaBio 1 (1), 94-104 , 2025
    2025
  • IDENTIFIKASI MIKROSKOPIK FUNGI PADA BIJI JAGUNG (Zea Mays L.) MENGGUNAKAN METODE PEWARNAAN
    AZ Diasti, ED Pratisara, RH Pratiwi
    Prosiding Semnas PenaBio 1 (1), 48-56 , 2025
    2025
  • Pengaruh Gaya Belajar dan Efikasi Diri Terhadap Kemampuan Berpikir Kreatif IPA (Expost Facto pada SMP Negeri di Jakarta Utara)
    I Mulyani, A Suryana, RH Pratiwi
    ALFARISI: Jurnal Pendidikan MIPA 8 (2), 151-159 , 2025
    2025
    Citations: 1
  • Community Service On Environmental Education: Introducing English Through Ecolinguistics Games Among Rural Children
    J Maratis, U Salamah, RH Pratiwi
    International Journal of Community Care of Humanity (IJCCH) 3 (7) , 2025
    2025
  • Genocide Education from The Perspective of International Law: A Cross-Cultural Approach to Prevent Crimes Against Humanity
    P Chandra, MBT Nur, R Septiani, RH Pratiwi, E Susylowati
    International Journal of Community Care of Humanity (IJCCH) 3 (7) , 2025
    2025
  • Penerapan Konsep Pembelajaran MIPA Topik Usaha dan Energi: Studi Pustaka
    M Anindyta, F Sodik, N Herdiansyah, M Sofiandi, RH Pratiwi
    Algoritma: Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa 3 … , 2025
    2025
  • PKM STRATEGI PENGGUNAAN MENDELEY UNTUK MENURUNKAN ANGKA PLAGIASI DI INSTITUSI PENDIDIKAN
    RH Pratiwi, ST Simorangkir, RN Salamah, GM Pangrestu, D Murtado
    Jurnal Media Akademik (JMA) 3 (7) , 2025
    2025
  • Perbandingan Efektivitas Pembelajaran Deep Learning dan Diferensiasi terhadap Kemampuan Pemecahan Masalah dan Penalaran Matematika Siswa SMP
    D Dahroni, ZA Saputra, H Restiani, M Ayu, RH Pratiwi
    Aljabar: Jurnal Ilmuan Pendidikan, Matematika Dan Kebumian 1 (3), 124-140 , 2025
    2025
    Citations: 8

MOST CITED SCHOLAR PUBLICATIONS

  • Mekanisme pertahanan bakteri patogen terhadap antibiotik
    RH Pratiwi
    Pro-life 4 (3), 418-429 , 2017
    2017
    Citations: 297
  • Potensi kapuk randu (Ceiba pentandra Gaertn.) dalam penyediaan obat herbal
    RH Pratiwi
    E-Journal Widya Kesehatan dan Lingkungan 1 (1), 36809 , 2014
    2014
    Citations: 65
  • Pengaruh kecerdasan intrapersonal terhadap kemampuan pemecahan masalah pada mata pelajaran biologi
    P Zahra, E Gresinta, RH Pratiwi
    EduBiologia: Biological Science and Education Journal 1 (1), 48-54 , 2021
    2021
    Citations: 43
  • Metode pembelajaran ‘make a match’dan pengaruhnya terhadap hasil belajar IPA
    RH Pratiwi
    Florea J. Biol. dan Pembelajarannya 5 (1), 37 , 2018
    2018
    Citations: 42
  • Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima
    RH Pratiwi, I Hidayat, M Hanafi, W Mangunwardoyo
    Journal of Microbiology 55 (4), 289-295 , 2017
    2017
    Citations: 34
  • Dasar-dasar mikrobiologi dan penerapannya
    DGS Harahap, A Noviantari, R Hidana, NA Yanti, ED Nugroho, ...
    Penerbit Widina , 2021
    2021
    Citations: 31
  • Prevalensi dan jenis masalah emosional dan perilaku pada anak usia 9-11 tahun dengan perawakan pendek di Kabupaten Brebes
    FA Rahmadi, G Hardaningsih, R Pratiwi
    Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) 3 (2), 116-119 , 2015
    2015
    Citations: 27
  • Dampak Pengelolaan Sampah di TPA Cipayung pada Kesehatan Masyarakat
    E Emilda, NAP Septiani, RH Pratiwi
    WAWASAN KESEHATAN: JURNAL ILMIAH ILMU KESEHATAN 5 (1) , 2019
    2019
    Citations: 26
  • Analisis Tingkat Serangan Parasit Pada Ikan Nila (Oreochromis niloticus) dan Ikan Lele (Clarias gariepinus) di Balai Benih Ikan (BBI) Ciganjur
    I Yuliani, RH Pratiwi
    Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 68-80 , 2023
    2023
    Citations: 23
  • Infectivity of lytic phage to enteropathogenic Escherichia coli from diarrheal patients in Indonesia
    S Budiarti, RH Pratiwi, I Rusmana
    J US-China Med Sci 8 (5), 273-282 , 2011
    2011
    Citations: 22
  • Analisis pemecahan masalah biologi berdasarkan kemampuan berpikir kritis peserta didik SMA Kelas XI IPA
    PR Endang, RH Pratiwi, TA Sari
    EduBiologia: Biological Science and Education Journal 1 (2), 149-156 , 2021
    2021
    Citations: 21
  • Distribusi Bakteri Coliform di SITU Cilodong Depok Jawa Barat
    RH Pratiwi
    Faktor Exacta 6 (4), 290-297 , 2015
    2015
    Citations: 21
  • Peranan mikroorganisme endofit dalam dunia kesehatan: kajian pustaka
    RH Pratiwi
    Sainmatika: Jurnal Ilmiah Matematika Dan Ilmu Pengetahuan Alam 16 (1), 21-32 , 2019
    2019
    Citations: 17
  • Bioactivity of antibacterial compounds produced by endophytic actinomycetes from Neesia altissima
    RH Pratiwi, M Hanafi, N Artanti, RD Pratiwi
    Journal of Tropical Life Science 8 (1), 228144 , 2018
    2018
    Citations: 17
  • Identification and characterization of three endophytic bacteria from Neesia altissima (Malvaceae) antagonistic to diarrhea-causing bacteria
    RH Pratiwi, I Hidayat, M Hanafi, W Mangunwardoyo
    Malaysian Journal of Microbiology, 300-307 , 2016
    2016
    Citations: 16
  • Analisis kemampuan berpikir kritis siswa smp terhadap mata pelajaran ipa melalui penggunaan media google classroom
    M Meriyanti, RH Pratiwi, E Gresinta, E Sulistyaniningsih
    Diklabio: Jurnal Pendidikan Dan Pembelajaran Biologi 5 (2), 226-232 , 2021
    2021
    Citations: 15
  • Isolation and structure elucidation of phenazine derivative from Streptomyces sp. strain UICC B-92 isolated from Neesia altissima (Malvaceae)
    RH Pratiwi, I Hidayat, M Hanafi, W Mangunwardoyo
    Iranian Journal of Microbiology 12 (2), 127-137 , 2020
    2020
    Citations: 15
  • Analisis Morfologi Fungi pada Tempe Kemasan Daun dan Tempe Kemasan Plastik
    R Rosidah, AS Azizah, HP Megawati, R Rivaldi
    Jurnal Pengabdian Masyarakat Biologi Dan Sains 2 (1), 48-57 , 2023
    2023
    Citations: 12
  • Kesehatan lingkungan
    RH Pratiwi, S Darmayani, S Salbiah, N Siahaya, SBR Perangin-angin, ...
    CV WIDINA MEDIA UTAMA , 2022
    2022
    Citations: 12
  • Antimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae).
    RH Pratiwi, E Oktarina, W Mangunwardoyo, I Hidayat, E Saepudin
    Pharmacognosy Journal 14 (1) , 2022
    2022
    Citations: 9