Immunology and Microbiology, Medicine, General Immunology and Microbiology
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Scopus Publications
Scopus Publications
Asthma outcomes in adults with preserved ratio impaired spirometry: a cohort study Cintia Bertagni Mingotti, Alcides Rocha, Monique Olivia Burch, Marcos José Oliveira da Silva, Jessica Regina Bertolino, Daniel Antunes, Ronei Luciano Mamoni, Eduardo V. Ponte Erj Open Research, 2026 <title>Extract</title> Preserved Ratio Impaired Spirometry (PRISm) is usually characterized by reduced FEV<sub>1</sub> accompanied by a FEV<sub>1</sub>/FVC ratio above the lower limit of normal (LLN) [1, 2]. Some studies, however, have labeled it as a “restrictive” pattern when “PRISm” is accompanied by a reduction in Forced Vital Capacity (FVC) [3, 4]. Possibly, PRISm with normal FVC would be a borderline pattern between normal spirometry and airway obstruction because, in this scenario, a low FEV<sub>1</sub> value results in FEV<sub>1</sub>/FVC ratio close to the LLN; but without crossing the threshold that defines obstruction.
Trajectory of the response to bronchodilator and respiratory outcomes in adults with asthma-like symptoms Daniel Gimenez da Rocha, Monique Olivia Burch, Luciana Aparecida Teixeira Soares, Jessica Regina Bertolino, Ana Lúcia Bergamasco Galastri, Daniel Antunes, Ronei Luciano Mamoni, Eduardo Vieira Ponte Monaldi Archives for Chest Disease, 2026 In the real world, health professionals need to care for individuals with asthma-like symptoms who have a persistently negative bronchodilator response (BDR). Little is known about the evolution of symptoms and lung function of these individuals because they are usually excluded from studies on asthma. The aim of this study was to evaluate whether individuals with asthma-like symptoms but with a persistently negative BDR have a different evolution of symptoms and lung function compared to individuals with asthma proven by positive BDR. This prospective cohort study included adults with asthma-like symptoms. Individuals participated in two visits, 12 months apart. They responded to questionnaires and underwent a spirometry test. In individuals without airway obstruction in the first visit, those with asthma-like symptoms and persistently negative BDR were less likely to lose forced expiratory volume in the first second during follow-up or progress to airway obstruction at the final visit compared to individuals with asthma proven by positive BDR. Among individuals with airway obstruction at baseline, those with asthma-like symptoms and persistently negative BDR were less likely to resolve the airway obstruction during follow-up compared to individuals with asthma proven by positive BDR. In individuals with proven asthma, the emergence or persistence of positive BDR during follow-up was accompanied by a worsening of asthma outcomes compared to the remission of positive BDR. Thus, BRD is an accessible marker of disease progression in individuals with asthma-like symptoms. In individuals with asthma proven by positive BDR, the trend in BDR was associated with the evolution of symptoms and lung function.
Effect of intrapleural anti-Vascular Endothelial Growth Factor (VEGF) associated with nab paclitaxel in a murine model of malignant pleural effusion Carlos Sergio Rocha Silva, Lisete Ribeiro Teixeira, Karina Rocha Pereira, Philippe Figueiredo Braga Colares, Vanessa Adelia Alvarenga, Roberta Karla Barbosa Sales, Amanda Cabral Roque, Evaldo Marchi, Ronei Luciano Mamoni, Milena Marques Pagliarelli Acencio BMC Cancer, 2025 BACKGROUND: Malignant pleural effusion (MPE) is a disease associated with poor prognosis, high morbidity, and significant mortality. Murine models of MPE have successfully replicated its formation and metastases, providing valuable insights into potential therapeutic approaches. Nanoparticle-associated drugs (nab) offer the advantage of reduced toxicity while enhancing their effectiveness against tumor cells. This study aims to evaluate the efficacy of intrapleural nab-Paclitaxel, either alone or in combination with anti-vascular endothelial growth factor (VEGF), in a murine model of MPE. METHODS: Lewis Lung Carcinoma (LLC) cells were injected intrapleurally into 300 mice. After seven days, the mice were assigned to one of five treatment groups: intrapleural administration of Paclitaxel, nab-Paclitaxel, anti-VEGF, nab-Paclitaxel + anti-VEGF, or saline (untreated). Twenty animals per group were monitored weekly for weight, mobility, and survival. Additionally, ten mice from each group were euthanized on days 7, 14, 21 and 28 to assess pleural fluid volume, cytology, lactate dehydrogenase (LDH), interleukin-6 (IL-6), VEGF, transforming necrosis factor (TNF)-α, and histological characteristics. RESULTS: Pleural carcinomatosis was lethal across all groups; however, mice receiving nab-Paclitaxel + anti-VEGF exhibited the longest survival. Pleural fluid accumulation was most pronounced in the untreated and Paclitaxel groups, whereas the lowest levels were observed in the nab-Paclitaxel + anti-VEGF group. Tumor implantation in the pleura occurred in all groups, but the lowest scores were recorded in mice treated with nab-Paclitaxel + anti-VEGF. CONCLUSIONS: In this experimental MPE model, intrapleural administration of nab-Paclitaxel in combination with anti-VEGF significantly prolonged survival and reduced both pleural fluid volume and tumor implantation.
Body weight trajectory of non-obese asthmatics: Relationship with inhaled corticosteroids maintenance therapy Monique Olivia Burch, Daniel Gimenez Rocha, Leticia Belleze, Marcos Jose Oliveira Da Silva, Renata Pletsch Assuncao, Daniel Antunes, Ronei Luciano Mamoni, Eduardo Vieira Ponte Allergy and Asthma Proceedings, 2025 Background: It is unclear whether the continuous use of a high dose of inhaled corticosteroids (ICS) could contribute to an unfavorable trajectory in body weight. Objectives: The objective was to evaluate whether a high dose of ICS used continuously for maintenance therapy increases the risk of an unfavorable body weight trajectory in individuals with asthma and who are not obese. Methods: We screened consecutive individuals with chronic respiratory symptoms suggestive of asthma who underwent a medical consultation in any of the 42 public health facilities in the municipality of Jundiaí, Brazil. We included individuals with proven asthma who were ≥ 20 years of age and who had a body mass index (BMI) < 30 kg/m2 on the day of screening for the study. Individuals participated in two study visits 12 months apart, named V1 and V2. Between study visits, individuals had one intermediate consultation with their referring physician. Results: Ninety-nine individuals used a high dose of ICS during the study and 294 used a low-medium dose. The individual with asthma and no obesity and who used a high dose of ICS had a similar risk of having obesity at V2 compared with those who used a low-medium dose (adjusted odds ratio 1.18 [95% confidence interval, 0.46‐3.04]). The probability of gaining weight was similar between the two groups. Conclusion: The use of a high dose of ICS for 1 year does not increase the risk of obesity among individuals with asthma and who are not obese, nor is it associated with an unfavorable body weight trajectory compared with individuals with asthma who were using a low-medium dose of ICS. This information may help reduce fears about using ICS.
Association between Chronic Obstructive Pulmonary Disease and Hearing Loss with Impaired Speech Recognition: A Cross-Sectional Study Leticia Belleze, Monique Olivia Burch, Luciana Aparecida Teixeira Soares, Viviane Cristina Martori Pandini, Raquel Prestes, Jessica Regina Bertolino, Ronei Luciano Mamoni, Eduardo Vieira Ponte Audiology and Neurotology, 2024 Introduction: Studies have identified a greater risk of sensory neural hearing loss in individuals with chronic obstructive pulmonary disease (COPD) compared to healthy individuals, but it is unclear whether they are at increased risk of hearing loss with impaired speech recognition. The aim of this study was to assess whether COPD is associated with hearing loss that affects speech recognition. Methods: This is a case-control study. We screened individuals from health facilities in the municipality of Jundiai. We enrolled a test group of individuals with COPD and an age-matched control group composed of individuals with asthma. The selected individuals attended an appointment with a chest physician, responded to questionnaires, and underwent tonal and speech audiometry. Adjusted binary logistic regression analysis evaluated whether COPD was associated with reduced speech recognition. Results: We enrolled 36 individuals with COPD and 72 with asthma. Individuals with COPD were more likely to have a reduced speech recognition compared to asthmatic individuals (reduced recognition of three-syllable words: adjusted OR 3.72, 95 CI [1.38–10.02]) (reduced recognition of monosyllable words: adjusted OR 4.74, 95 CI [1.52–14.76]). Conclusion: We conclude that individuals with COPD from primary and secondary healthcare facilities have at least 38% greater risk of hearing loss with reduced speech recognition compared to an age-matched control group of individuals with asthma recruited from the same facilities. We recommend that longitudinal studies evaluate whether regular screening could contribute to the prevention or early treatment of hearing loss in individuals with moderate-severe COPD.
White piedra on pediatric scalp: A case report Julia Brigagão de Carvalho Sugai, Nayara Pelizaro Di Rito, Alexandre Lourenço, Ronei Luciano Mamoni, Ana Carolina Da Mota Falleiros, Celia Antonia Xavier de Moraes Alves, Glaucos Ricardo Paraluppi Idcases, 2024 This case report describes a rare fungal infection, piedra alba, in a 5-year-old female initially misdiagnosed. Treatment with 2 % ketoconazole shampoo led to significant regression within a week, without the need for hair cutting. We discuss the importance of early and accurate diagnosis, highlighting potential hair damage and complications in immunocompromised cases. Dermatoscopy aided diagnosis, and 2 % ketoconazole demonstrated efficacy, emphasizing the need for a multidisciplinary approach and dermatological follow-up.
Ethanol modulates the effector functions of human monocyte-derived macrophages in response to Paracoccidioides brasiliensis yeast cells Lívia Furquim de Castro, Kamila de Araújo Mathias, Júlia Vieira Nunes, Ana Lúcia Bergamasco Galastri, Dennis Henrique Leandro da Silva, Larissa Nara Alegrini Longhi, Maria Heloisa de Souza Lima Blotta, Ronei Luciano Mamoni Medical Mycology, 2021 We aimed to investigate the effects of ethanol and its metabolites (β-hydroxybutyrate and sodium acetate) in the effector functions of macrophages in response to Paracoccidioides brasiliensis yeast cells and to determine their influence in the development of the adaptive response. Purified peripheral blood monocytes were differentiated into macrophages and were treated with ethanol, β-hydroxybutyrate, and sodium acetate, and stimulated with P. brasiliensis yeast cells and evaluated for their phenotypic characteristics, functional activity, and capability to induce T cells activation/differentiation. We found that the ethanol treatment diminished the expression of HLA-AB, HLA-DR, CD80, and CD86, modulating the expression of dectin-1, as well as Syk phosphorylation. The ethanol treatment increased the phagocytic activity, expression of CD206, and IL-10 production; however, reduced ROS production, fungicidal activity, caspase-1 cleavage, and IL-1β and IL-6 production. Our data also showed that the presence of ethanol reduced the differentiation of Th1 and Th17 cells and increased the frequency of Th2 cells. Our results indicated that ethanol exposure could suppress effector function of macrophages, possibly leading to the polarization of M2 macrophages. The ethanol modulates the expression of costimulatory and antigen-presentation molecules and interferes with the NLRP3 inflammasome. Altogether, these alterations affect the development of the adaptive response, decreasing the frequency of IL-17, IL-22, and IFN- γ producing cells, and increasing the frequency of IL-4 producing cells. Therefore, exposure to ethanol can impair the capability of macrophages to exert their effector functions and activate the acquired response related to resistance to P. brasiliensis infection.
Association between IL-27 and Tr1 cells in severe form of paracoccidioidomycosis Lívia Moreira Genaro, Lilian de Oliveira Coser, Amauri da Silva Justo-Junior, Lívia Furquim de Castro, Anne Karine Felício Barreto, Angela Eugênia Rizzato, Plínio Trabasso, Ronei Luciano Mamoni, Ricardo Mendes Pereira, Maria Letícia Cintra, Luana Nunes Santos, Murilo de Carvalho, Luciana Pereira Ruas, Maria Heloisa de Souza Lima Blotta Cytokine, 2020
Severe Paracoccidioidomycosis in a 14-Year-Old Boy L. P. Ruas, R. M. Pereira, F. G. Braga, X. T. Lima, R. L. Mamoni, M. L. Cintra, A. Z. Schreiber, V. L. G. Calich, M. H. S. L. Blotta Mycopathologia, 2016
Detection of TCD4+ subsets in human carotid atheroma Rômulo Tadeu Dias Oliveira, Rosiane Maria Silva, Fabio Haach Teo, Marcela Franco Mineiro, Maria Carolina Ferreira, Albina Altemani, Ronei Luciano Mamoni, Fábio Husseman Menezes, Maria Heloisa Souza Lima Blotta Cytokine, 2013
On the cytoadhesion of plasmodium vivax-infected erythrocytes Bruna O. Carvalho, Stefanie C. P. Lopes, Paulo A. Nogueira, Patricia P. Orlandi, Daniel Y. Bargieri, Yara C. Blanco, Ronei Mamoni, Juliana A. Leite, Mauricio M. Rodrigues, Irene S. Soares, Tatiane R. Oliveira, Gerhard Wunderlich, Marcus V. G. Lacerda, Hernando A. del Portillo, Maria O. G. Araújo, Bruce Russell, Rossarin Suwanarusk, Georges Snounou, Laurent Rénia, Fabio T. M. Costa Journal of Infectious Diseases, 2010
Cytokines in recurrent pregnancy loss Silvia Daher, Kátia de Arruda Geraldes Denardi, Maria Heloı́sa Souza Lima Blotta, Ronei Luciano Mamoni, Ana Paula Monteiro Reck, Luiz Camano, Rosiane Mattar Journal of Reproductive Immunology, 2004
