Aliev Shohjahon Tuxtamurodovich

@academy.uz

Molekulyar zoologiya va parazitologiya
Academy of Sciences of the Republic of Uzbekistan

Aliev Shohjahon Tuxtamurodovich


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https://researchid.co/shohjahon_1122

RESEARCH, TEACHING, or OTHER INTERESTS

Molecular Biology, Agricultural and Biological Sciences, Animal Science and Zoology, Ecology, Evolution, Behavior and Systematics
4

Scopus Publications

Scopus Publications

  • Morphological and Molecular-Genetic Characterization of the Nematode Oswaldocruzia filiformis Goeze, 1782 (Nematoda: Molineoidea) in the Amphibians of Southern and Central Uzbekistan
    Aliyev et al.
    Egyptian Journal of Aquatic Biology and Fisheries, 2025
  • Morphological and Molecular-Genetic Characterization of the Nematode Aplectana acuminata Schrank, 1788 (Nematoda: Cosmocercidae) in Uzbekistan
    Aliyev et al.
    Egyptian Journal of Aquatic Biology and Fisheries, 2025
    In this article, the partial nucleotides of the ribosomal DNA (rDNA) ITS region gene of A. acuminata nematodes were studied molecular-genetically. To conduct morphological and molecular-genetic research, 264 amphibian specimens were helminthologically examined from southern (Qashqadaryo and Surxondaryo regions) and central (Bukhara and Navoi regions) territories of Uzbekistan during 2023-2025. Morphological analyses revealed that the body length of male A. acuminata nematodes is 1.5- 3.6mm, while that of females is 3.3- 5.2mm; the muscular part of the esophagus forms a bulb, with the excretory pore located around it and opening after 0.26-0.37mm from the anterior part of the body. Comparative studies of our data and those of other authors determined that the width of the esophagus-intestine junction and the morphometric dimensions of the bulb circumference of A. acuminata nematodes are slightly larger. According to the results of molecular-genetic studies based on Kimura’s K2P model, transition substitutions (A↔G and C↔T) showed high frequencies, with an average distance of 13.6233. Transversion substitutions (A↔C, A↔T, G↔C, G↔T) demonstrated lower values, with an average result of 5.6883. The transition/transversion (Ti/Tv) ratio was ≈2.4. Neutrality test results based on 42 ITS sequences identified a total of 648 polymorphic sites. The calculated Tajima’s D value was 0.659444, indicating no significant deviation from neutral evolution. The phylogenetic tree of A. acuminata_uzb specimens belonging to the genus Aplectana Railliet & Henry, 1916, along with closely related representatives available in the NCBI database, formed 5 monophyletic groups with bootstrap values ranging from 51.2 to 100%. For the first time in Uzbekistan, the nucleotide sequence of the ITS region of rDNA belonging to A. acuminata_uzb nematodes was extracted and uploaded to the National Center for Biotechnology Information (NCBI) database by us. Based on morphological and molecular-genetic research results, this nematode species was confirmed to be a distinct, stable species.
  • Phylogenetic Relationships Classification of Bufotes pewzowi (Bedriaga, 1898) Inhabiting near Aquatic Basins of Central and South Uzbekistan
    Shohjahon et al.
    Egyptian Journal of Aquatic Biology and Fisheries, 2025
    This study analyzed partial nucleotide sequences of the mitochondrial DNA (mtDNA) 16S rRNA gene region in Bufotes pewzowi (Bedriaga, 1898). Over 580 nucleotides were amplified using PCR and subsequently sequenced. The resulting sequence data were compared with entries in the NCBI database and showed a 99.83% match with B. pewzowi. During the study, the nucleotide sequence of the mtDNA 16S rRNA gene for the B. pewzowi_Uzb specimen was submitted to NCBI and assigned the accession number PV124861. Molecular analysis confirmed that this specimen is a distinct species within the family Bufonidae, identified as B. pewzowi. Future research will enhance understanding of this species’ morphological traits and contribute to amphibian taxonomy. Phylogenetic analysis revealed that species within the Bufonidae family form four monophyletic groups, with bootstrap support values ranging from 50% to 100%. The monophyletic group containing the genus Bufotes, including B. pewzowi_Uzb, showed bootstrap support values between 75% and 100%, confirming its placement within the phylogenetic tree.
  • Morphological and Molecular-Genetic Classification of the Nematode Rhabdias engelbrechti Found in the Amphibian Pelophylax terentievi in the Aquatic Basins of South Uzbekistan
    Shohjahon et al.
    Egyptian Journal of Aquatic Biology and Fisheries, 2024