Study on Rapeseed Albumin Hydrolysis by PrtS Protease from Streptococcus thermophilus and Bioactivity Characterization of Resulting Hydrolysates Zeeshan Hafeez, Sophie Beaubier, Arnaud Aymes, Ségolène Christophe, Samina Akbar, et al. Foods, 2025 Lactic acid bacteria are well known for hydrolyzing milk proteins, but their application to plant proteins remains limited. This study evaluated the ability of the cell-wall-anchored PrtS protease from two Streptococcus thermophilus strains to hydrolyze rapeseed albumins (RAs), aiming to generate bioactive peptides with potential food functionality. The specific activity of PrtS was first determined using a chromogenic substrate. RAs were then hydrolyzed using 10X- and 100X-concentrated cell pellets of each strain to assess the hydrolysis kinetics and the enzymatic mechanism. The results showed concentration-dependent hydrolysis, with protein conversion and the degree of hydrolysis increasing threefold at 100X for both strains. Despite the increased hydrolysis, the peptides produced had similar average sizes, averaging at five amino acids, indicating a consistent “one-by-one” cleavage mechanism. The in vitro testing of the RA hydrolysates produced with 100X PrtS from S. thermophilus LMD-9 revealed dose-dependent antioxidant activity comparable to native RAs. Importantly, unlike native RAs, these hydrolysates did not induce increased secretion of the pro-inflammatory mediator IL-8 in inflamed HT-29 cells, suggesting a reduced pro-inflammatory potential. These findings demonstrate that PrtS protease from S. thermophilus can effectively hydrolyze rapeseed proteins to produce functional hydrolysates with improved bioactivity profiles. Such hydrolysates have promising applications as functional ingredients in plant-based food products, contributing both to health benefits and potential food preservation through antioxidant activity.
Optimization of Selective Hydrolysis of Cruciferins for Production of Potent Mineral Chelating Peptides and Napins Purification to Valorize Total Rapeseed Meal Proteins Nastassia Kaugarenia, Sophie Beaubier, Erwann Durand, Arnaud Aymes, Pierre Villeneuve, et al. Foods, 2022 Preventing oxidation and microbial spoilage are both major concerns in food industries. In this context, this study aimed to valorize the total rapeseed meal proteins with controlled enzymatic proteolysis to generate potent mineral-chelating peptides from cruciferins while keeping intact the antimicrobial napins. Implementation of proteolysis of total rapeseed protein isolate with the Prolyve® enzyme highlighted an interesting selective hydrolysis of the cruciferins. Hence, the mechanism of this particular hydrolysis was investigated through a Design of Experiments method to obtain a model for the prediction of kinetics (cruciferin degradation and napin purity) according to the operating conditions applied. Then, multicriteria optimization was implemented to maximize the napin purity and yield while minimizing both enzymatic cost and reaction time. Antioxidant assays of the peptide fraction obtained under the optimal conditions proved the high metal-chelating activity preservation (EC50 = 247 ± 27 µg) for more than three times faster production. This fraction might counteract lipid oxidation or serve as preventing agents for micronutrient deficiencies, and the resulting purified napins may have applications in food safety against microbial contamination. These results can greatly help the development of rapeseed meal applications in food industries.
Role of interfacial elasticity (E′) as an indicator for the foam characteristics of plant proteins MM Assaf, P Marchal, F Michaux, V Sadtler, S Beaubier, R Kapel, ... Journal of Molecular Liquids, 129676 , 2026 2026
Mechanistic and kinetic modeling of enzymatic crosslinking of rapeseed albumins: influence of temperature, pH, and enzyme concentration R Faure, S Beaubier, G Génin, R Kapel Journal of Food Engineering, 113163 , 2026 2026
Extraits protéiques de lies de vin blanc: vers de nouveaux agents de collage pour les vins rouges: Article prenant sa source de l’article de recherche “White wine lees protein … L Garcia, A Douliez, B Poulain, L Gagnepain, F Gallo, R Kapel, ... IVES Technical Reviews, vine and wine , 2026 2026
White Wine Lees Protein Extract: A Promising Fining Agent for Red Wine Clarification L Garcia, A Douliez, B Poulain, L Gagnepain, F Gallo, R Kapel, ... LWT, 118867 , 2025 2025
Enzymatic crosslinking of rapeseed albumins: kinetic monitoring, physical characteristics, and functional property enhancements R Faure, R Kapel, P Marchal, G Génin, S Beaubier Food Hydrocolloids, 112100 , 2025 2025 Citations: 2
Study on Rapeseed Albumin Hydrolysis by PrtS Protease from Streptococcus thermophilus and Bioactivity Characterization of Resulting Hydrolysates Z Hafeez, S Beaubier, A Aymes, S Christophe, S Akbar, R Kapel, L Miclo Foods 14 (13), 2235 , 2025 2025 Citations: 5
Study of the in vitro digestibility of oilseed protein concentrates compared to isolates for food applications S Beaubier, S Albe-Slabi, L Beau, O Galet, R Kapel Food Chemistry 464, 141737 , 2025 2025 Citations: 5
The role of extraction and purification conditions in flaxseed protein isolate production and its emulsifying properties S Albe-Slabi, O Mesieres, S Beaubier, L Beau, A Aymes, ... LWT 215, 117170 , 2025 2025 Citations: 7
Chelating peptides from rapeseed meal protein hydrolysates: identification and evaluation of their capacity to inhibit lipid oxidation S Beaubier, E Durand, C Lenclume, F Fine, A Aymes, X Framboisier, ... Food chemistry 422, 136187 , 2023 2023 Citations: 20
Improving the in vitro digestibility of rapeseed albumins resistant to gastrointestinal proteolysis while preserving the functional properties using enzymatic hydrolysis S Beaubier, C Pineda-Vadillo, O Mesieres, X Framboisier, O Galet, ... Food Chemistry 407, 135132 , 2023 2023 Citations: 42
Optimization of selective hydrolysis of cruciferins for production of potent mineral chelating peptides and napins purification to valorize total rapeseed meal proteins N Kaugarenia, S Beaubier, E Durand, A Aymes, P Villeneuve, F Lesage, ... Foods 11 (17), 2618 , 2022 2022 Citations: 8
Using the dual isotope method to assess cecal amino acid absorption of goat whey protein in rats, a pilot study J Calvez, N Khodorova, S Beaubier, A Eymard, D Tomé, C Gaudichon Amino acids 54 (5), 811-821 , 2022 2022 Citations: 3
Multiobjective decision making strategy for selective albumin extraction from a rapeseed cold-pressed meal based on Rough Set approach S Beaubier, C Defaix, S Albe-Slabi, A Aymes, O Galet, F Fournier, ... Food and Bioproducts Processing 133, 34-44 , 2022 2022 Citations: 7
Antioxidant chelating peptides production from Rapeseed meal proteins proteolysis E Durand, P Villeneuve, N Kaugarenia, N Barouh, R Kapel Journal of the American Oil Chemists' Society 99 (S1), 113-114 , 2022 2022
Optimization of potent mineral chelating peptides production from rapeseed meal proteins proteolysis and peptide characterizations N Kaugarenia, S Beaubier, E Durand, F Lesage, X Framboisier, A Aymes, ... Journal of the American Oil Chemists' Society 99 (S1), 167-168 , 2022 2022
Selective extraction of napins: Process optimization and impact on structural and functional properties S Albe-Slabi, C Defaix, S Beaubier, O Galet, R Kapel Food Hydrocolloids 122, 107105 , 2022 2022 Citations: 28
Study of Linseed Protein Extraction and Purification: Impact of Process Conditions on Protein Functional and Structural Properties. SA Slabi, S Beaubier, M Ndiaye, O Galet, R Kapel JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 98, 100-100 , 2021 2021
High metal chelating properties from rapeseed meal proteins to counteract lipid oxidation in foods: controlled proteolysis and characterization E Durand, S Beaubier, F Fine, P Villeneuve, R Kapel European journal of lipid science and technology 123 (6), 2000380 , 2021 2021 Citations: 21
A rational approach for the production of highly soluble and functional sunflower protein hydrolysates S Beaubier, S Albe-Slabi, A Aymes, M Bianeis, O Galet, R Kapel Foods 10 (3), 664 , 2021 2021 Citations: 27
A new approach for modelling and optimizing batch enzymatic proteolysis S Beaubier, X Framboisier, F Fournier, O Galet, R Kapel Chemical Engineering Journal 405, 126871 , 2021 2021 Citations: 27
MOST CITED SCHOLAR PUBLICATIONS
Production and antioxidant capacity of bioactive peptides from plant biomass to counteract lipid oxidation E Durand, S Beaubier, I Ilic, R Kapel, P Villeneuve Current Research in Food Science 4, 365-397 , 2021 2021 Citations: 71
Simultaneous quantification of the degree of hydrolysis, protein conversion rate and mean molar weight of peptides released in the course of enzymatic proteolysis S Beaubier, X Framboisier, I Ioannou, O Galet, R Kapel Journal of Chromatography B 1105, 1-9 , 2019 2019 Citations: 54
Improving the in vitro digestibility of rapeseed albumins resistant to gastrointestinal proteolysis while preserving the functional properties using enzymatic hydrolysis S Beaubier, C Pineda-Vadillo, O Mesieres, X Framboisier, O Galet, ... Food Chemistry 407, 135132 , 2023 2023 Citations: 42
Selective extraction of napins: Process optimization and impact on structural and functional properties S Albe-Slabi, C Defaix, S Beaubier, O Galet, R Kapel Food Hydrocolloids 122, 107105 , 2022 2022 Citations: 28
A rational approach for the production of highly soluble and functional sunflower protein hydrolysates S Beaubier, S Albe-Slabi, A Aymes, M Bianeis, O Galet, R Kapel Foods 10 (3), 664 , 2021 2021 Citations: 27
A new approach for modelling and optimizing batch enzymatic proteolysis S Beaubier, X Framboisier, F Fournier, O Galet, R Kapel Chemical Engineering Journal 405, 126871 , 2021 2021 Citations: 27
High metal chelating properties from rapeseed meal proteins to counteract lipid oxidation in foods: controlled proteolysis and characterization E Durand, S Beaubier, F Fine, P Villeneuve, R Kapel European journal of lipid science and technology 123 (6), 2000380 , 2021 2021 Citations: 21
Chelating peptides from rapeseed meal protein hydrolysates: identification and evaluation of their capacity to inhibit lipid oxidation S Beaubier, E Durand, C Lenclume, F Fine, A Aymes, X Framboisier, ... Food chemistry 422, 136187 , 2023 2023 Citations: 20
Ultrafiltration fractionation of bovine hemoglobin hydrolysates: Prediction of separation performances for optimal enrichment in antimicrobial peptide S Beaubier, R Przybylski, A Bodin, N Nedjar, P Dhulster, R Kapel Membranes 11 (2), 73 , 2021 2021 Citations: 19
Optimization of selective hydrolysis of cruciferins for production of potent mineral chelating peptides and napins purification to valorize total rapeseed meal proteins N Kaugarenia, S Beaubier, E Durand, A Aymes, P Villeneuve, F Lesage, ... Foods 11 (17), 2618 , 2022 2022 Citations: 8
The role of extraction and purification conditions in flaxseed protein isolate production and its emulsifying properties S Albe-Slabi, O Mesieres, S Beaubier, L Beau, A Aymes, ... LWT 215, 117170 , 2025 2025 Citations: 7
Multiobjective decision making strategy for selective albumin extraction from a rapeseed cold-pressed meal based on Rough Set approach S Beaubier, C Defaix, S Albe-Slabi, A Aymes, O Galet, F Fournier, ... Food and Bioproducts Processing 133, 34-44 , 2022 2022 Citations: 7
Study on Rapeseed Albumin Hydrolysis by PrtS Protease from Streptococcus thermophilus and Bioactivity Characterization of Resulting Hydrolysates Z Hafeez, S Beaubier, A Aymes, S Christophe, S Akbar, R Kapel, L Miclo Foods 14 (13), 2235 , 2025 2025 Citations: 5
Study of the in vitro digestibility of oilseed protein concentrates compared to isolates for food applications S Beaubier, S Albe-Slabi, L Beau, O Galet, R Kapel Food Chemistry 464, 141737 , 2025 2025 Citations: 5
Using the dual isotope method to assess cecal amino acid absorption of goat whey protein in rats, a pilot study J Calvez, N Khodorova, S Beaubier, A Eymard, D Tomé, C Gaudichon Amino acids 54 (5), 811-821 , 2022 2022 Citations: 3
Enzymatic crosslinking of rapeseed albumins: kinetic monitoring, physical characteristics, and functional property enhancements R Faure, R Kapel, P Marchal, G Génin, S Beaubier Food Hydrocolloids, 112100 , 2025 2025 Citations: 2
Role of interfacial elasticity (E′) as an indicator for the foam characteristics of plant proteins MM Assaf, P Marchal, F Michaux, V Sadtler, S Beaubier, R Kapel, ... Journal of Molecular Liquids, 129676 , 2026 2026
Mechanistic and kinetic modeling of enzymatic crosslinking of rapeseed albumins: influence of temperature, pH, and enzyme concentration R Faure, S Beaubier, G Génin, R Kapel Journal of Food Engineering, 113163 , 2026 2026
Extraits protéiques de lies de vin blanc: vers de nouveaux agents de collage pour les vins rouges: Article prenant sa source de l’article de recherche “White wine lees protein … L Garcia, A Douliez, B Poulain, L Gagnepain, F Gallo, R Kapel, ... IVES Technical Reviews, vine and wine , 2026 2026
White Wine Lees Protein Extract: A Promising Fining Agent for Red Wine Clarification L Garcia, A Douliez, B Poulain, L Gagnepain, F Gallo, R Kapel, ... LWT, 118867 , 2025 2025