Sunitha Katta
@gitam.edu
Assistant Professor- Pharmacognosy and Phytochemistry
GITAM Institute of Pharmacy, GITAM Deemed to be University
RESEARCH INTERESTS
Isolation and Characterization of secondary metabolites from medicinal plants.
Pharmacological screening of plant extracts and Phytoconstituents
Development and evaluation of herbal formulations
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
T. Sravani and Sunitha Katta
Asian Journal of Chemistry
In present investigation, the aerial parts of two fern species viz. Polystichum acrostichoides (PA) and Adiantum lunulatum (AL) were used to evaluate the potent antidiabetic activity. Methanol solvent was used for extraction by soxhlation method followed by LC-MS analysis to identify the presence of compounds in both the extracts. Further, isolation of the major compounds was carried out and characterized by IR, mass and NMR techniques. Thereafter, in vitro α-amylase inhibitory activity for both the extracts was determined by comparing with acarbose as standard. Thereafter, α-glucosidase inhibition activity followed by glucose uptake was studied using L6 cell line study. Practical yield for both the fern extracts was calculated and observed A. lunulatum extract showed more yield (7 g) than P. acrostichoides (3 g). Based on the analytical data, isolated compound was identified as quercetin and kaempferol from the A. lunulatum and P. acrostichoides extracts, respectively. A. lunulatum extract even showed better dose dependent α-amylase inhibition and α-glucosidase inhibition activity than P. acrostichoides furthermore, L6 cell line study showed increased glucose uptake by A. lunulatum extract than P. acrostichoides extract when compared with standard insulin. Based on the findings, it appears that the both A. lunulatum and P. acrostichoides have the potential to be used as antidiabetic drugs due to presence of major isolated flavonoids i.e. quercetin and kaempferol as among the bioactive compounds. Antidiabetic efficacy was dosage dependent and A. lunulatum extract was more effective in inhibiting glucose uptake than P. acrostichoides extract.
Akram Hussain, Revoju Sravanthi, Sunitha Katta, and Dhevalapally B. Ramachary
Royal Society of Chemistry (RSC)
A simple two-step dialkylation protocol was developed to synthesize antibiotics photopyrones, pseudopyronines, and violapyrones from triacetate lactone in excellent yields.
Poornima Gurivelli and Sunitha Katta
Egypts Presidential Specialized Council for Education and Scientific Research
T. Sravani and K. Sunitha
Indian Association of Biomedical Scientists
Introduction and Aim: Free radicals have been established detrimental effects on human health, mostly because of oxidative stress. Antioxidants are essential in reducing these effects and protecting cells from oxidative damage. As a result, there is rising interest in finding natural antioxidants. In this work, Polystichum acrostichoides and Adiantum lunulatum aerial parts, both widely utilized in medical systems for their therapeutic effects and as sources of free radicals, were examined to see how solvent extraction affected their antioxidant activity.
 
 Materials and Methods: In both plants' methanolic and hexane extracts, the amounts of phenolic and flavonoid compounds were measured. Utilizing the ABTS, DPPH, and hydroxyl radical scavenging tests, the antioxidant activity of the extracts was evaluated.
 
 Results: In both plant species, the methanolic extracts had higher phenolic and flavonoid contents than the hexane extracts. Notably, the ABTS, DPPH, and hydroxyl radical scavenging assays revealed that the methanol extract had the best antioxidant potency. On the other hand, there was no apparent antioxidant action in the hexane extracts.
 
 Conclusion: The results suggest that the aerial parts of Polystichum acrostichoides and Adiantum lunulatum could be effective sources of antioxidants. In the ABTS, DPPH, and hydroxyl radical scavenging experiments, the methanolic extracts had reduced IC50 values, which indicated that they had considerable antioxidant activity. These results demonstrate the potential of these ferns in the creation of innovative treatments for a range of oxidative stress-related diseases.
Udaya C P and Sunitha K
Dr. Yashwant Research Labs Pvt. Ltd.
Aspidopterys indica belongs to the family Malphigiaceae and is used to treat hypertension and skin diseases. In the current investigation, methanolic extract was fractionated using vacuum liquid chromatography, and the methanolic fraction was subjected to column chromatography for isolation and characterized by fourier transform infrared spectroscopy (FTIR), Mass, proton nuclear magnetic resonance (1H-NMR), and carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy. The compounds isolated were assessed for their in-vitro DPPH antioxidant activity. Two compounds, AI-1 and AI-2, established after spectral analysis, were identified as catechin flavan-3-ol and isoorientin flavone C-glucoside. The results indicated that the compounds showed concentration-dependant DPPH scavenging action and IC50 of catechin 93.66 μg/mL, isoorientin 92.09 μg/mL. This study concludes that catechin and isoorientin with known antioxidant potential were isolated from the methanolic fraction of A. indica, which could effectively manage oxidative stress-related diseases.
Steve Thomas Pannakal, Ganapaty Seru, Sunitha Katta, Alekya Veeramaneni, and Hartmut Laatsch
Elsevier BV
Krishnaphanisri Ponnekanti, Sunitha K., and Ganapaty S.
A and V Publications
A new rapid, accurate, precise and economical reverse phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Methylcobalamin, Epalrestat and Pregabalin in bulk and pharmaceutical dosage form. The separation was accomplished utilizing Agilent C18, 150 x 4.6mm, 5 column at a detection wavelength of 210nm utilizing the mobile phase water and acetonitrile 60: 40 v/v at the flow rate of 0.8ml/min and injection volume of 10µl. The total run time was 6.0 min. Validation discovered the method was specific, rapid, accurate, precise, reliable and reproducible. The calibration curve was linear over the concentration range of 0.37 – 2.25μg/ml of Methylcobalamin, 37.5-225μg/ml of Epalrestat and 37.5-225μg/ml of Pregabalin respectively with correlation coefficient of 0.999. The accuracy was determined by recovery studies and was found to be 99.5-100%. The precision of the results stated that the %RSD was <2%. The limits of detection for Methylcobalamin, Epalrestat and Pregabalin were 0.2, 0.9 and 1.2μg/ml, while the limits of quantification were 0.5, 1.5 and 0.9μg/ml respectively. Forced degradation study was carried out under acidic, alkaline, oxidative, photolytic and thermal conditions to prove the stability-indicating ability of the developed HPLC method. The high recovery confirms the suitability of developed method and can be further used in routine analysis.
KRISHNAPHANISRI PONNEKANTI and K. SUNITHA
Innovare Academic Sciences Pvt Ltd
Objective: Azelnidipine (AZEL) and Telmisartan (TELM) combination is referred to the sufferers of hypertension. No analytical process has yet been mentioned for the TELM and AZEL combination analysis. We, therefore, have designed for its first time stability demonstrating methodology based on HPLC for analysing TELM and AZEL in the tablets and bulk. Methods: The assay of TELM and AZEL was get done on a 250 mm length C18 column (Supelco, 4.6 mm inner diameter, 5.0 μm particle size), and utilized 0.1M Na2SO4 (pH 3.6) and acetonitrile (55% volume: 45% volume) as the mobile solvents phase, at a stream rate 1.0 ml/min. HPLC recognition of TELM and AZEL was taken by a photodiode array sensor set at 258 nm. For validation of the stability demonstrating methodology proposed in terms of sensitivity, precision, specificity, linearity, device adequacy, robustness and accuracy, ICH directives were followed. Results: Calibration curves of TELM and AZEL were generated in the array of 20-60 µg/ml and 4-12 µg/ml with recovery percentage ranges of 99.62%-101.05% and 97.76%-100.17%, and detection limits of 0.020 µg/ml and 0.009 µg/ml, respectively. TELM and AZEL stability was inspected in the existence of acid, base, light, heat, and oxidation and it was realised to be more stable under oxidation degradation testing conditions employed when compared to acid, alkaline, photo, and heat degradation testing conditions applied. Conclusion: The observations demonstrated that the described HPLC stability demonstrating methodology was suitable for quantitating TELM and AZEL combination in tablets and bulk.
P. Naresh, B. Pramodh, S. Naveen, S. Ganguly, J. Panda, K. Sunitha, W. Maniukiewicz, and N.K. Lokanath
Elsevier BV
Abstract Chalcone derivatives are simple chemical scaffold found in natural products and have been extensively used as an effective template in medicinal chemistry for drug discovery. Two new geometrical isomers of 1-(5-bromothiophen-2-yl)-3-(10-chloroanthracen-9-yl)prop-2-en-1-one were synthesized, isolated and characterized by various spectroscopic techniques (FT-IR, NMR, LC-MS and UV-visible). Two different shaped (cis and trans) yellow colored single crystals were obtained by slow evaporation method and the molecular structures were confirmed by X-ray diffraction studies. The cis configuration crystallizes in the monoclinic P21/c space group, whereas, trans configuration crystallizes in the orthorhombic P212121 space group. The conformational changes lead to variations in molecular packing arrangement. In cis, 2-D layer-like architecture is constructed by C-H…O and S…S contacts, whereas, in trans 3-D zig-zag pattern is generated by C-H…O, C-H…Cl and Br…π interactions. The unique supramolecular topology of the isomers established by diverse intra and intermolecular interactions was analysed by Hirshfeld surfaces, 2D fingerprint plots, 3D energy framework and NCI index model. The Frontier molecular orbitals explored HOMO-LUMO energy gap and associated electronic properties were calculated. The molecular electrostatic potential (MEP) was plotted to identify the reactive sites in the molecule.
Alekhya V., Ganapaty S., and Sunitha K.
A and V Publications
The aim of the present study was to evaluate pharmacognostical and antimicrobial, antioxidant, anti-inflammatory activity of ethanolic leaf extracts of Alternanthera pungens. The collected plants were washed, dried, cut and ground into coarse powder and macerated with ethanol and extract was dried by rotary vacuum. Phytochemical screening of ethanol extract revealed the presence of steroids, flavonoids, saponins, glycoside, phenols, and proteins. However, alkaloids and glycosides were absence in this species. The ethanolic extract of whole plant of A. pungens was subjected to column chromatography to separate pure compounds. Invitro antioxidant activity was performed by 2, 2 -diphenyl- 1- picrylhydrazyl (DPPH) assay, hydroxy radical scavenging method and superoxide radical scavenging activity. The ethanolic extract of A. pungens was evaluated against antimicrobial activity against two fungal and four bacterial strains. The extracts showed remarkable inhibition of zone of bacterial and results were comparable with that of standard drugs against the organism tested.
Krishnaphanisri Ponnekanti and K. Sunitha
Asian Journal of Chemistry
In this investigation, a highly reliable, precise, stability indicating, specific and selective RP-HPLC approach with photodiode array detection (RP-HPLC-PDA) was established to determine simultaneously imipenem, cilastatin and relebactum in bulk drug and injection dose forms. Chromatographic separation of imipenem, cilastatin and relebactum was achieved via using C18 XTerra column and a mobile phase poised of acetonitrile and 0.1 M dipotassium hydrogen phosphate buffer (4.5 pH, set with 0.1% orthophosphoric acid) at 45:55 (v/v) ratio with a flow stream of 1 mL/min. The photodiode array detector was fixed at wavelength 245 nm and quantifications of imipenem, cilastatin and relebactum were based on assessing their peak response areas. Good linearity was detected in target range concentrations of 250-750 μg/mL (imipenem and cilastatin) and 125-375 μg/mL (relebactum). The precision (standard variation percentage) was between 0.141% and 0.257%. Accuracy (%assay nominal) determined was between 99.144% and 99.638%. The validated RP-HPLC approach was applied to Recarbio injection dose evaluating imipenem, cilastatin and relebactum content with no interference encountered from the injection dose inactive ingredients. Imipenem, cilastatin and relebactum were subjected to forced conditions like 30% peroxide, 0.1 N NaOH, sunlight, 0.1 N HCl and 60 ºC. Imipenem, cilastatin and relebactum were effectively separated, quantified and resolved from the degradants generated in forced conditions.
M. Sebastin Santhosh, M. Shanmuga Sundaram, K. Sunitha, S. Jnaneshwari, S. Devaraja, K. Kemparaju, and K. S. Girish
Springer Science and Business Media LLC
Mahalingam S. Sundaram, Mahadevappa Hemshekhar, Martin S. Santhosh, Manoj Paul, Kabburahalli Sunitha, Ram M. Thushara, Somanathapura K. NaveenKumar, Shivanna Naveen, Sannaningaiah Devaraja, Kanchugarakoppal S. Rangappa,et al.
Springer Science and Business Media LLC
AbstractMedicinal plants are employed in the treatment of human ailments from time immemorial. Several studies have validated the use of medicinal plant products in arthritis treatment. Arthritis is a joint disorder affecting subchondral bone and cartilage. Degradation of cartilage is principally mediated by enzymes like matrix metalloproteinases (MMPs), hyaluronidases (HAase), aggrecanases and exoglycosidases. These enzymes act upon collagen, hyaluronan and aggrecan of cartilage respectively, which would in turn activate bone deteriorating enzymes like cathepsins and tartrate resistant acid phosphatases (TRAP). Besides, the incessant action of reactive oxygen species and the inflammatory mediators is reported to cause further damage by immunological activation. The present study demonstrated the anti-arthritic efficacy of tamarind seed extract (TSE). TSE exhibited cartilage and bone protecting nature by inhibiting the elevated activities of MMPs, HAase, exoglycosidases, cathepsins and TRAP. It also mitigated the augmented levels of inflammatory mediators like interleukin (IL)-1β, tumor necrosis factor-α, IL-6, IL-23 and cyclooxygenase-2. Further, TSE administration alleviated increased levels of ROS and hydroperoxides and sustained the endogenous antioxidant homeostasis by balancing altered levels of endogenous antioxidant markers. Overall, TSE was observed as a potent agent abrogating arthritis-mediated cartilage/bone degradation, inflammation and associated stress in vivo demanding further attention.
K. Sunitha, M. Hemshekhar, R.M. Thushara, M. Sebastin Santhosh, M. Shanmuga Sundaram, K. Kemparaju, and K.S. Girish
Elsevier BV
S. Jnaneshwari, M. Hemshekhar, R.M. Thushara, M. Sundaram, M. Santhosh, K. Sunitha, R.L. Shankar, K. Kemparaju, and K.S. Girish
Bentham Science Publishers Ltd.
In current scenario of human health and diseases, drug-induced hepatic injury has been recognized as a serious and unresolved problem. Particularly, chemotherapeutic agents have been reported to induce organ toxicity. The aim of the present study is to evaluate organ toxicity and oxidative damage induced by cyclophosphamide (CP), a chemotherapeutic drug and its amelioration by sesamol, an antioxidant from sesame seeds. CP (150 mg/kg) is injected intraperitonially to experimental rats and from day 2 rats were orally treated with sesamol. Rats were sacrificed to evaluate non-enzymatic and enzymatic oxidative stress parameters in serum and tissue homogenates on day 8. Besides, liver function parameters and pro-inflammatory mediators were assessed. Histopathological studies of liver and kidney were also carried out. Elevated levels of endogenous reactive oxygen species, lipid peroxidation and decreased levels of glutathione, total thiols, along with the reduction in antioxidant enzymes including superoxide dismutase, catalase, glutathione-stransferase and glutathione peroxidase, were evident in CP-intoxicated animals. Pro-inflammatory mediators like tumor necrosis factor - α, interleukin (IL)-1β, IL-6 and cyclooxygenase-2 were also elevated. Moreover, the levels of liver function markers like serum alanine aminotransferase and aspartate aminotransferase were also altered. Histology of liver and kidney tissues further supported CP-induced organ damage. Altered parameters were significantly restored to normal by oral administration of sesamol (50 mg/kg) suggesting its antioxidative stress, anti-inflammatory and hepatoprotective abilities. The study clearly demonstrated the potentiality of sesamol against CPinduced organ toxicity and oxidative stress suggesting its applicability in treatment regime of cancer and other stress-associated disorders as a supportive/auxiliary therapy.
G. D. Katkar, M. Shanmuga Sundaram, M. Hemshekhar, D. Rachana Sharma, M. Sebastin Santhosh, K. Sunitha, K. S. Rangappa, K. S. Girish, and K. Kemparaju
Wiley
AbstractViper bites cause high morbidity and mortality worldwide and regarded as a neglected tropical disease affecting a large healthy population. Classical antivenom therapy has appreciably reduced the snakebite mortality rate; it apparently fails to tackle viper venom‐induced local manifestations that persist even after the administration of antivenom. Recently, viper venom‐induced oxidative stress and vital organ damage is deemed as yet another reason for concern; these are considered as postmedicated complications of viper bite. Thus, treating viper bite has become a challenge demanding new treatment strategies, auxiliary to antivenin therapy. In the last decade, several studies have reported the use of plant products and clinically approved drugs to neutralize venom‐induced pharmacology. However, very few attempts were undertaken to study oxidative stress and vital organ damage. Based on this background, the present study evaluated the protective efficacy of melatonin in Echis carinatus (EC) venom‐induced tissue necrosis, oxidative stress, and organ toxicity. The results demonstrated that melatonin efficiently alleviated EC venom‐induced hemorrhage and myonecrosis. It also mitigated the altered levels of inflammatory mediators and oxidative stress markers of blood components in liver and kidney homogenates, and documented renal and hepatoprotective action of melatonin. The histopathology of skin, muscle, liver, and kidney tissues further substantiated the overall protection offered by melatonin against viper bite toxicities. Besides the inability of antivenoms to block local effects and the fact that melatonin is already a widely used drug promulgating a multitude of therapeutic functionalities, its use in viper bite management is of high interest and should be seriously considered.
M. Sebastin Santhosh, R. M. Thushara, M. Hemshekhar, K. Sunitha, S. Devaraja, K. Kemparaju, and K. S. Girish
Springer Science and Business Media LLC
R.M. Thushara, M. Hemshekhar, K. Sunitha, M.S. Kumar, S. Naveen, K. Kemparaju, and K.S. Girish
Elsevier BV
M. Sebastin Santhosh, M. Shanmuga Sundaram, K. Sunitha, K. Kemparaju, and K. S. Girish
Springer Science and Business Media LLC