TANIA GUADALUPE PENAFLOR GALINDO

@nagaoka-ct.ac.jp

APPOINTED ASSISTANT PROFESSOR/ GENERAL DEPARTMENT
NATIONAL INSTITUTE OF TECHNOLOGY, NAGAOKA COLLEGE

https://researchid.co/taniagpe

RESEARCH, TEACHING, or OTHER INTERESTS

Ceramics and Composites, Materials Science, Chemistry, Water Science and Technology

Scopus Publications

259

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Surface functionalization of hydroxyapatite nanoparticles for biomedical applications
Takuya Kataoka, Zizhen Liu, Iori Yamada, Tania Guadalupe Peñaflor Galindo, and Motohiro Tagaya
Royal Society of Chemistry (RSC)
This review described the highlight of surface engineering of hydroxyapatite nanoparticles and their interactions with ions and molecules, and summarized how the surface functionalization effectively work in biological situations.

Investigation of Surface Layers on Biological and Synthetic Hydroxyapatites Based on Bone Mineralization Process
Kazuto Sugimoto, Yanni Zhou, Tania Guadalupe Peñaflor Galindo, Reo Kimura, and Motohiro Tagaya
MDPI AG
In this review, the current status of the influence of added ions (i.e., SiO44−, CO32−, etc.) and surface states (i.e., hydrated and non-apatite layers) on the biocompatibility nature of hydroxyapatite (HA, Ca10(PO4)6(OH)2) is discussed. It is well known that HA is a type of calcium phosphate with high biocompatibility that is present in biological hard tissues such as bones and enamel. This biomedical material has been extensively studied due to its osteogenic properties. The chemical composition and crystalline structure of HA change depending on the synthetic method and the addition of other ions, thereby affecting the surface properties related to biocompatibility. This review illustrates the structural and surface properties of HA substituted with ions such as silicate, carbonate, and other elemental ions. The importance of the surface characteristics of HA and its components, the hydration layers, and the non-apatite layers for the effective control of biomedical function, as well as their relationship at the interface to improve biocompatibility, has been highlighted. Since the interfacial properties will affect protein adsorption and cell adhesion, the analysis of their properties may provide ideas for effective bone formation and regeneration mechanisms.

Drug Molecular Immobilization and Photofunctionalization of Calcium Phosphates for Exploring Theranostic Functions
Iori Yamada, Kota Shiba, Tania Guadalupe Peñaflor Galindo, and Motohiro Tagaya
MDPI AG
Theranostics (bifunction of therapeutics and diagnostics) has attracted increasing attention due to its efficiency that can reduce the physical and financial burden on patients. One of the promising materials for theranostics is calcium phosphate (CP) and it is biocompatible and can be functionalized not only with drug molecules but also with rare earth ions to show photoluminescence that is necessary for the diagnostic purpose. Such the CP-based hybrids are formed in vivo by interacting between functional groups of organic molecules and inorganic ions. It is of great importance to elucidate the interaction of CP with the photofunctional species and the drug molecules to clarify the relationship between the existing state and function. Well-designed photofunctional CPs will contribute to biomedical fields as highly-functional ormultifunctional theranostic materials at the nanoscales. In this review, we describe the hybridization between CPs and heterogeneous species, mainly focusing on europium(III) ion and methylene blue molecule as the representative photofunctional species for theranostics applications.

Investigation of Inclusion States of Silicate and Carbonate Ions in Hydroxyapatite Particles Prepared under the Presence of Sodium Silicate
Tania Guadalupe Peñaflor Galindo, Kazuto Sugimoto, Shota Yamada, Taito Sugibuchi, Zizhen Liu, and Motohiro Tagaya
MDPI AG
Biological hydroxyapatite (HA) contains the different minor ions which favour its bio-reactivity in vivo. In this study, the preparation of HA particles containing both silicate and carbonate ions under the presence of sodium silicate was investigated, and the physicochemical properties were evaluated according to the contents and states of silicate and carbonate ions. The increment in the silicate ion reduced the crystallinity and expanded the crystalline size along with a-axis. Solid-state 29Si–NMR spectra indicated the increase in the adsorption of oligomeric silicate species on the HA particle surfaces in addition to the substitution state of silicate ions, suggesting the occurrence of the surface coating of silicates on the surfaces. The possible states of carbonate and silicate ions at the HA surfaces will provide the bioactivity.

Surface modification of hydroxyapatite nanoparticles for bone regeneration by controlling their surface hydration and protein adsorption states
Zizhen Liu, Shota Yamada, Yuichi Otsuka, Tania Guadalupe Peñaflor Galindo, and Motohiro Tagaya
Royal Society of Chemistry (RSC)
The control of the hydration and protein adsorption states on hydroxyapatite surface was systematically discussed, which is very important for the proper understanding of the controllable interfacial interactions between cells and bioceramics.

Effect of orotic acid addition on hydrolysis conversion of α-tricalcium phosphate to hydroxyapatite
Iori Yamada, Tania Guadalupe Peñaflor Galindo, Daichi Noda, and Motohiro Tagaya
Wiley
AbstractAlpha‐tricalcium phosphate (α‐TCP), which can be converted to calcium‐deficient hydroxyapatite (HA) by hydrolysis reaction, has been researched as a bone filling. Although the composites of α‐TCP with various organic molecules can exhibit a novel functionality, the reaction based on the addition of the interactive organic molecules has not been understood. Investigation of the hydrolysis conversion process under various conditions can lead to the elucidation of the biomineralization process. In this study, we investigated the hydrolysis conversion of α‐TCP to HA in the presence of orotic acid (Ort). As a result, the conversion rate from α‐TCP to HA at the reaction time of 3 h was improved to be more than five times as compared to that in the absence of Ort. Moreover, the addition of Ort promoted the crystal growth and suppressed the crystal growth in the m‐plane direction. Furthermore, the mechanism for promoting HA formation is assumed to be related to the molecular structure of Ort, which is capable of forming the interactions between carboxylate and Ca2+ ions.

Synthesis of Luminescent Eu(III)-Doped Octacalcium Phosphate Particles Hybridized with Succinate Ions and Their Reactive Behavior in Simulated Body Fluid
Iori Yamada, Daichi Noda, Kenji Shinozaki, Tania Guadalupe Peñaflor Galindo, and Motohiro Tagaya
American Chemical Society (ACS)
We successfully synthesized the photofunctionalized octacalcium phosphate (OCP) with layered structures and high biosafety. Specifically, two systems of europium(III) ion (Eu3+)-doped OCP particles...

Interfacial Effect of Hydration Structures of Hydroxyapatite Nanoparticle Films on Protein Adsorption and Cell Adhesion States
Tania Guadalupe Peñaflor Galindo and Motohiro Tagaya
American Chemical Society (ACS)
The synthesized elliptical hydroxyapatite (E-HAp) and needle-like HAp (N-HAp) nanoparticles (NPs) were electrophoretically deposited on a gold (Au) substrate. A comparative study of the hydration layers on E-HAp, N-HAp, and Au films was achieved to investigate the interfacial effect of the hydration layers on the conformation of the adsorbed fibrinogen (Fgn) and fibroblast adhesion properties. As a result, the ratios of three types of hydration layer states (free water, intermediate water, nonfreezing water) analyzed by a Fourier transform infrared (FT-IR) spectral deconvolution of the O-H stretching absorption band were investigated. The ratio of the bonding water state (i.e., intermediate and nonfreezing water molecules) is almost the same between two HAp films, and the E-HAp film with an elliptical shape and smaller particle size exhibited the smallest ratio of nonfreezing water, which can suppress the denaturation of the adsorbed protein. Subsequently, FT-IR spectral deconvolution results of the amide I band of the adsorbed Fgn on the E-HAp film indicated the higher proportion of α-helix and β-sheet structures as compared with those on the N-HAp and Au films, suggesting that the smaller proportion of nonfreezing waters would play a significant role in the stereoscopic Fgn conformation. In the culture of fibroblasts, FT-IR spectra of the adhered cells on the E-HAp, N-HAp, and Au films exhibited different absorbance intensities of the amide A, I, II, and III bands, suggesting a different amount of collagen-producing states by the cells, which were also supported by immunostaining results of the collagen type I. Therefore, the different hydration structures on the films clearly influenced the conformation of the adsorbed protein, and the preferential conformation was found at the interfaces between the fibroblasts and the underground E-HAp films.

Biodiesel production using layered double hidroxides and derived mixed oxides: The role of the synthesis conditions and the catalysts properties on biodiesel conversion
L.R. Reyna-Villanueva, J. M. Dias, N.A. Medellín-Castillo, R. Ocampo-Pérez, J.M. Martínez-Rosales, T. Peñaflor-Galindo, and G. Alvarez Fuentes
Elsevier BV

Hydroxyapatite nanoparticle coating on polymer for constructing effective biointeractive interfaces
Tania Guadalupe Peñaflor Galindo, Yadong Chai, and Motohiro Tagaya
Hindawi Limited
Bone is an organic-inorganic composite with the ability to regenerate itself. Thus, several studies based on artificial organic-inorganic interface sciences have been tried to develop capable materials for effective regenerative bone tissues. Hydroxyapatite nanoparticles (HAp NPs) have extensively been researched in bone tissue engineering due to the compositional and shape similarity to the mineral bone and excellent biocompatibility. However, HAp alone has low mechanical strength, which limits its applications. Therefore, HAp NPs have been deposited on the biocompatible polymer matrix, obtaining composites with the enhanced mechanical, thermal, and rheological properties and with higher biocompatibility and bioactivity. For developing new biomedical applications, polymer-HAp interfacial interactions that provide biofusion should be investigated. This paper reviewed common coating techniques for obtaining HAp NPs/polymer fusion interfaces as well as in vitro studies of interfacial interactions with proteins and cells, demonstrating better biocompatibility. Studies based on interfacial interactions between biomolecules and HAp NPs were highlighted, and how these interactions can be affected by specific protein preadsorption was also summarized.

Studies on preparation of surfactant-assisted elliptical hydroxyapatite nanoparticles and their protein-interactive ability
Tania Guadalupe Peñaflor Galindo, Iori Yamada, Shota Yamada, and Motohiro Tagaya
Elsevier BV

Osteoblast-like cell growth and differentiation behaviors on the phospholipid vesicle-interacted calcium phosphate films
Yadong Chai, Tadashi Yamaguchi, Tania Guadalupe Penaflor Galindo, and Motohiro Tagaya
Surface Science Society Japan
The culture behavior of the osteoblast-like cells on the calcium phosphate (CP) films interacted with phospholipid vesicle (PV) were investigated. The different CP crystalline phases were prepared from the biological solutions such as simulated body fluid (SBF) and unique SBF, which were called as CP/PV and U-CP/PV films, respectively, and these were used after the sterilization. The protein adsorption amounts on the CP/PV films were higher than that of the PV film. The osteoblast-like cells cultured on the U-CP/PV film had the longest length along with the extension direction. The density of the osteoblast-like cells adhered on the CP/PV and U-CP/PV films were higher, suggesting the preferential adhesion on the surfaces. The differentiated osteoblasts on the films were visualized by the alkaline phosphatase and Alizarin-red-S staining methods. It was suggested that the osteoblasts cultured on the CP/PV and U-CP/PV films exhibited the possibility of the high osteogenic activities. Therefore, the PV-interacted CP films prepared from the biological solutions are useful for the osteoblast culture plates. [DOI: 10.1380/ejssnt.2018.156]

Modification of poly(dimethylsiloxane) by mesostructured siliceous films for constructing protein-interactive surfaces
Yuri Maruko, Tania Guadalupe Penaflor Galindo, and Motohiro Tagaya
Surface Science Society Japan
We investigated the coating process of mesostructured triblock copolymer-tetraethoxysilane (P123-TEOS) and mesoporous silica (MPS) films on a bioinert poly(dimethylsiloxane) (PDMS) with the different cross-linker concentrations through an oxygen-plasma treatment to evaluate the mesostructure formation and adsorption ability of proteins (albumin, fibrinogen, γ-globulin, fetal bovine serum). In the PDMS preparation, the cross-linker concentration affected the polymer network formation and the siliceous layer was formed on the most-surfaces by the plasma treatment. The transparent siliceous films of P123-TEOS and MPS were successfully covered on the cross-linked PDMS without voiding and the coating film thicknesses were ca. 100 nm. The FT-IR spectra indicated that the change from P123-TEOS to MPS occurred with preserving the PDMS chemical bonds by the calcination. Especially, the XRD patterns and nitrogen adsorption and desorption isotherms of the MPS on PDMS indicated the mesostructured film formation with preserving the ordered nanopore structures (BJH pore sizes: 1.6–4.2 nm, BET surface areas: 394–602 m/g). The hydrophobic PDMS surfaces became more wettable by the coating. The adsorption amounts of acidic proteins (albumin, fibrinogen) were changed by the coating. For the fibrinogen, the P123-TEOS on PDMS exhibited the most adsorption sites. Therefore, the bio-interactive properties of the PDMS surfaces were demonstrated based on the coating processes. [DOI: 10.1380/ejssnt.2018.41]

Particulate Titania Coating on Poly(Dimethylsiloxane) Films for Improving Osteoconductive Ability
Tania Guadalupe Peñaflor Galindo, Kota Shiba, and Motohiro Tagaya
Trans Tech Publications, Ltd.
The cytocompatibility of the poly (dimethylsiloxane) (PDMS) surfaces can be improved by the coating with biomaterials. In this study, the methodology for the particulate titania (PT) coating on the PDMS film was investigated via the combined process of microfluidic synthesis system with spin-coating, leading to the one-step synthesis and coating. The PT was successfully deposited on the O2-plasma-treated PDMS films by mixing titanium tetraisopropoxide, isopropyl alcohol, water and octadecylamine in a microfluidic reactor and subsequently dropping. The rotation speed in the spin-coating plays an important role in the PT morphologies and deposition amounts on the PDMS films. Through the detailed investigation, the efficient condition for adhering PT to PDMS as well as inducing apatite formation from simulated body fluid was successfully discovered.

Preparation of nanocrystalline zinc-substituted hydroxyapatite films and their biological properties
Tania Guadalupe Peñaflor Galindo, Takuya Kataoka, Shuji Fujii, Mitushiro Okuda, and Motohiro Tagaya
Elsevier BV

Morphosynthesis of Zn-Substituted Stoichiometric and Carbonate Hydroxyapatite Nanoparticles and Their Cytotoxicity in Fibroblasts
Tania Guadalupe Peñaflor Galindo, Takuya Kataoka, and Motohiro Tagaya
Hindawi Limited
Hydroxyapatite (Ca10(PO4)3(OH)2) (HAp) is crystallographically and chemically similar to the human hard tissues and has been widely researched. The naturally formed HAp has some impurities of some ions, which provides the biocompatibility as well as the nanosized morphologies in the tissues. In this study, the morphosynthesis of zinc-substituted stoichiometric and carbonate hydroxyapatite (Zn:HAp and Zn:CAp) nanoparticles was investigated from the reagents of CaCl2, ZnCl2, and K2HPO4. The initial (Ca + Zn)/P ratios of 1.67 and 2.00 were adjusted by the initial ZnCl2amount at the Zn/(Ca + Zn) concentration of 0.0−10 mol%. The crystalline sizes of the nanoparticles decreased with increasing the Zn ion amount, suggesting that the Zn substitution significantly suppressed the crystal growth. TEM images of the nanoparticles indicated that all the crystalline sizes are less than 100 nm and the needle-like shapes were significantly changed to spherical shapes with increasing the Zn ion substitution to resultantly exhibit the higher surface areas as well as the nanoparticle aggregation states. Furthermore, all the nanoparticle films electrically plated on a silicone substrate give no cytotoxicity, and the Zn:CAp nanoparticle films significantly provided the bioactive properties for fibroblast ingrowth, suggesting the effect of Zn and carbonate ions on the cytocompatibility.

RECENT SCHOLAR PUBLICATIONS

Surface State Control of Apatite Nanoparticles by pH Adjusters for Highly Biocompatible Coatings
K Sugimoto, R Akutsu, S Yamada, TG Peñaflor Galindo, M Tagaya
ACS Applied Materials & Interfaces 2025

Surface functionalization of hydroxyapatite nanoparticles for biomedical applications
T Kataoka, Z Liu, I Yamada, TGP Galindo, M Tagaya
Journal of Materials Chemistry B 12 (28), 6805-6826 2024

Investigation of surface layers on biological and synthetic hydroxyapatites based on bone mineralization process
K Sugimoto, Y Zhou, TGP Galindo, R Kimura, M Tagaya
Biomimetics 8 (2), 184 2023

Drug molecular immobilization and photofunctionalization of calcium phosphates for exploring theranostic functions
I Yamada, K Shiba, TGP Galindo, M Tagaya
Molecules 27 (18), 5916 2022

Investigation of Inclusion States of Silicate and Carbonate Ions in Hydroxyapatite Particles Prepared under the Presence of Sodium Silicate
TG Peaflor Galindo, K Sugimoto, S Yamada, T Sugibuchi, Z Liu, ...
Biomimetics 7 (2), 40 2022

Surface modification of hydroxyapatite nanoparticles for bone regeneration by controlling their surface hydration and protein adsorption states
Z Liu, S Yamada, Y Otsuka, TGP Galindo, M Tagaya
Dalton Transactions 51 (25), 9572-9583 2022

AEMac, Autonomous Evolution Machine: A Leap for Innovative Problem Solving
KR Sone- Yamagishi, P Sariego-Pasten, PGT Guadalupe
International Journal of Scientific Research and Engineering Development 4 2021

Effect of orotic acid addition on hydrolysis conversion of α‐tricalcium phosphate to hydroxyapatite
I Yamada, TGP Galindo, D Noda, M Tagaya
International Journal of Ceramic Engineering & Science 3 (6), 267-271 2021

Synthesis of luminescent Eu (III)-doped octacalcium phosphate particles hybridized with succinate ions and their reactive behavior in simulated body fluid
I Yamada, D Noda, K Shinozaki, TG Peñaflor Galindo, M Tagaya
Crystal Growth & Design 21 (4), 2005-2018 2021

Interfacial Effect of Hydration Structures of Hydroxyapatite Nanoparticle Films on Protein Adsorption and Cell Adhesion States
TG Peñaflor Galindo, M Tagaya
ACS Applied Bio Materials 2 (12), 5559-5567 2019

Hydroxyapatite nanoparticle coating on polymer for constructing effective biointeractive interfaces
TGP Galindo, Y Chai, M Tagaya
Journal of Nanomaterials 2019 (1), 6495239 2019

Studies on preparation of surfactant-assisted elliptical hydroxyapatite nanoparticles and their protein-interactive ability
TGP Galindo, I Yamada, S Yamada, M Tagaya
Materials Chemistry and Physics 221, 367-376 2019

Particulate Titania Coating on Poly (Dimethylsiloxane) Films for Improving Osteoconductive Ability
TG Peaflor Galindo, K Shiba, M Tagaya
Key Engineering Materials 782, 151-157 2018

Osteoblast-like Cell Growth and Differentiation Behaviors on the Phospholipid Vesicle-Interacted Calcium Phosphate Films
Y Chai, T Yamaguchi, TGP Galindo, M Tagaya
e-Journal of Surface Science and Nanotechnology 16, 156-162 2018

Modification of poly (dimethylsiloxane) by mesostructured siliceous films for constructing protein-interactive surfaces
Y Maruko, TGP Galindo, M Tagaya
e-Journal of Surface Science and Nanotechnology 16, 41-48 2018

Desarrollo de una metodologa para sintetizar el composito PDMS/TiO2/HAp con habilidades osteoconductivas
TGP Galindo
Universidad de Guanajuato 2018

Sntesis de Materiales de Alto Impacto y Bajo Costo para Remocin De [F-] De Agua Para Consumo
DMV Zamarripa, TGP Galindo, JMM Rosales
JVENES EN LA CIENCIA 2 (1), 289-293 2016

Preparation of nanocrystalline zinc-substituted hydroxyapatite films and their biological properties
TGP Galindo, T Kataoka, S Fujii, M Okuda, M Tagaya
Colloid and Interface Science Communications 10, 15-19 2016

Morphosynthesis of Zn‐Substituted Stoichiometric and Carbonate Hydroxyapatite Nanoparticles and Their Cytotoxicity in Fibroblasts
TG Peaflor Galindo, T Kataoka, M Tagaya
Journal of Nanomaterials 2015 (1), 376045 2015

Synthesis, Characterization and Evaluation of Hydroxyapatite Doped with Zinc for Several Applications
TGP GALINDO
Universidad de Guanajuato 2015

MOST CITED SCHOLAR PUBLICATIONS

Hydroxyapatite nanoparticle coating on polymer for constructing effective biointeractive interfaces
TGP Galindo, Y Chai, M Tagaya
Journal of Nanomaterials 2019 (1), 6495239 2019
Citations: 97

Biodiesel production using layered double hidroxides and derived mixed oxides: The role of the synthesis conditions and the catalysts properties on biodiesel conversion
GAF L.R. Reyna-Villanueva, J. M. Dias N.A. Medelln-Castillo, R. Ocampo ...

Citations: 26

Investigation of surface layers on biological and synthetic hydroxyapatites based on bone mineralization process
K Sugimoto, Y Zhou, TGP Galindo, R Kimura, M Tagaya
Biomimetics 8 (2), 184 2023
Citations: 11

Drug molecular immobilization and photofunctionalization of calcium phosphates for exploring theranostic functions
I Yamada, K Shiba, TGP Galindo, M Tagaya
Molecules 27 (18), 5916 2022
Citations: 4

Surface functionalization of hydroxyapatite nanoparticles for biomedical applications
T Kataoka, Z Liu, I Yamada, TGP Galindo, M Tagaya
Journal of Materials Chemistry B 12 (28), 6805-6826 2024
Citations: 2