Veena Awadhesh Singh

@kth.se

Postdoctoral Fellow, Department of material Science and Engineering
KTH Royal Institute of Technology, Stockholm, Sweden

Veena Awadhesh Singh


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https://researchid.co/veenas

EDUCATION

PhD. in Chemistry (2018) Indian Institute of Technology Banaras Hindu University, Varanasi, India
MSc: (2012) Organic Chemistry, University of Pune, Maharashtra, India
BSc: (2010) Chemistry, University of Pune, Maharashtra, India

RESEARCH INTERESTS

Material synthesis, Energy storage, Battery and Supercapacitor, Thin film deposition, Inkjet technology, Magnetic sputtering, Additive manufacturing, Photocatalysis, Solar cells, Renewable energy resources execution, Biofuels, Biodiesel production
56

Scopus Publications

837

Scholar Citations

10

Scholar h-index

10

Scholar i10-index

Scopus Publications

  • Detection of oral squamous cell carcinoma using indigenously developed multimodal optical imaging and spectroscopy device
    Veena Singh, Pramila Thapa, Vivek Nayyar, Varun Surya, Deepika Mishra, et al.
    Proceedings of SPIE the International Society for Optical Engineering, 2025
  • Two-channel In-vivo Early-stage Oral Cancer Screening Using Field-portable Multimodal and Multispectral Device: A Label-free Approach
    Pramila Thapa, Veena Singh, Varun Surya, Deepika Mishra, Anuj Saxena, et al.
    Progress in Biomedical Optics and Imaging Proceedings of SPIE, 2025
  • Multimodal and multispectral diagnostic devices for oral and breast cancer screening in low resource settings
    Dalip Singh Mehta, Pramila Thapa, Veena Singh, Himanshu Joshi, Dibya Jyoti Sarangi, et al.
    Current Opinion in Biomedical Engineering, 2023
  • Multimodal fluorescence imaging and spectroscopic techniques for oral cancer screening: a real-time approach
    Pramila Thapa, Veena Singh, Sunil Bhatt, Kiran Maurya, Virendra Kumar, et al.
    Methods and Applications in Fluorescence, 2023
    The survival rate of oral squamous cell carcinoma (OSCC) patients is very poor, but it can be improved using highly sensitive, specific, and accurate techniques. Autofluorescence and fluorescence techniques are very sensitive and helpful in cancer screening; being directly linked with the molecular levels of human tissue, they can be used as a quantitative tool for cancer detection. Here, we report the development of multi-modal autofluorescence and fluorescence imaging and spectroscopic (MAF-IS) smartphone-based systems for fast and real-time oral cancer screening. MAF-IS system is indigenously developed and offers the advantages of being a low-cost, handy, non-contact, non-invasive, and easily operable device that can be employed in hospitals, including low-resource settings. In this study, we report the results of 43 individuals with 28 OSCC and 15 oral potentially malignant disorders (OPMDs), i.e., epithelial dysplasia and oral submucous fibrosis, using the developed devices. We observed a red shift in fluorescence emission spectra in vivo. We found red-shift of 7.72 ± 6 nm, 3 ± 4.36 nm, and 1.33 ± 0.47 nm in the case of OSCC, epithelial dysplasia, and oral submucous fibrosis, respectively, compared to normal. The results were compared with histopathology and found to be consistent. Further, the MAF-IS system provides results in real-time with higher accuracy and sensitivity compared to devices using a single modality. Our system can achieve an accuracy of 97% with sensitivity and specificity of 100% and 94.7%, respectively, even with a smaller number of patients (28 patients of OSCC). The proposed MAF-IS device has great potential for fast screening and diagnosis of oral cancer in the future.
  • Point-of-care devices based on fluorescence imaging and spectroscopy for tumor margin detection during breast cancer surgery: Towards breast conservation treatment
    Pramila Thapa, Veena Singh, Komal Gupta, Anurag Shrivastava, Virendra Kumar, et al.
    Lasers in Surgery and Medicine, 2023
    OBJECTIVE Fluorescence-based methods are highly specific and sensitive and have potential in breast cancer detection. Simultaneous fluorescence imaging and spectroscopy during intraoperative procedures of breast cancer have great advantages in detection of tumor margin as well as in classification of tumor to healthy tissues. Intra-operative real-time confirmation of breast cancer tumor margin is the aim of surgeons, and therefore, there is an urgent need for such techniques and devices which fulfill the surgeon's priorities. METHODS In this article, we propose the development of fluorescence-based smartphone imaging and spectroscopic point-of-care multi-modal devices for detection of invasive ductal carcinoma in tumor margin during removal of tumor. These multimodal devices are portable, cost-effective, noninvasive, and user-friendly. Molecular level sensitivity of fluorescence process shows different behavior in normal, cancerous and marginal tissues. We observed significant spectral changes, such as, red-shift, full-width half maximum (FWHM), and increased intensity as we go towards tumor center from normal tissue. High contrast in fluorescence images and spectra are also recorded for cancer tissues compared to healthy tissues. Preliminary results for the initial trial of the devices are reported in this article. RESULTS A total 44 spectra from 11 patients of invasive ductal carcinoma (11 spectra for invasive ductal carcinoma and rest are normal and negative margins) are used. Principle component analysis is used for the classification of invasive ductal carcinoma with an accuracy of 93%, specificity of 75% and sensitivity of 92.8%. We obtained an average 6.17 ± 1.66 nm red shift for IDC with respect to normal tissue. The red shift and maximum fluorescence intensity indicates p < 0.01. These results described here are supported by histopathological examination of the same sample. CONCLUSION In the present manuscript, simultaneous fluorescence-based imaging and spectroscopy is accomplished for the classification of IDC tissues and breast cancer margin detection.
  • Analysis of early-stage breast cancer using smartphone-based fluorescence imaging and spectroscopy during intra-operative procedure
    Pramila Thapa, Veena Singh, Komal Gupta, Anurag Srivastava, Dalip S. Mehta
    Progress in Biomedical Optics and Imaging Proceedings of SPIE, 2023
    Breast cancer is most common cancer among women world-wide and can be treated if diagnosed at early-stage. Fluorescence (FL) techniques have a considerable impact in tumor detection as FL is highly sensitive to biochemical and biophysical structure of the tissues, providing novel techniques for early and noninvasive diagnosis of cancer. Due to high sensitivity of FL, it can be used in early-stage breast cancer detection. Fluorescence-guided-surgery (FGS) using exogenous agent in breast cancer is a well-known method, which locates tumor and margins during intra-operative procedure. FL-spectroscopy and FL-imaging has shown the potentiality in FGS of breast cancer independently. Combination of these two modalities in a single system improves the success rate of FGS which can give tumor free tissues. The goal of the current study is to analyze and classify the early-stage breast cancer, locally advanced breast cancer (invasive ductal carcinoma) and normal tissue. For that, we combine FL-imaging and FL-spectroscopy in a single smartphone-based point-of-care devices and recorded data during intra-operative procedure. A total 21 patients of invasive ductal carcinoma and fibroadenoma are included in this study. Total 65 FL-spectra are recorded during intra-operative procedure which are further used in support vector machine (SVM) based classification of fibroadenoma, invasive ductal carcinoma (IDC) and normal tissue. The sensitivity, specificity, positive predictive value, negative predictive value, and overall diagnostic accuracy (total efficiency) is 78.6 %, 90%, 91.6%, 75%, 95%, respectively. Additionally, we observed a red shift in case of IDC and fibroadenoma from normal tissue, which is 5.22±1.77 nm, and 4.96±2.61 nm, respectively.
  • Design and development of integrated TIRF and common-path quantitative phase microscopic health care system with high stability
    Shilpa Tayal, Anuj Saxena, Veena Singh, Tejinder Kaur, Neetu Singh, et al.
    Optics and Lasers in Engineering, 2022
  • White light phase shifting interferometric microscopy with whole slide imaging for quantitative analysis of biological samples
    Priyanka Mann, Veena Singh, Shilpa Tayal, Pramila Thapa, Dalip Singh Mehta
    Journal of Biophotonics, 2022
    In this paper, we demonstrate the white light phase shifting Interferometer employed as whole slide scanner and phase profiler for determining qualitative and quantitative information over large field-of-view. Experiments were performed on human erythrocytes and MG63 Osteosarcoma cells. Here, we have recorded microscopic images and phase shifted white light interferograms simultaneously in a stepped manner. Sample slide is translated in transverse direction such that there exists a correlation between the adjacent frames, and they were stitched together using correlation functions. Final stitched image has a field-of-view of 0.24*1.14 mm with high resolution ~0.8 μm. Circular Hough transform algorithm is implemented to the resulting image for cell counting and five-step phase shifting algorithm is utilised to retrieve the phase profiles over a large field-of-view. Further, this technique is utilised to study the difference between normal and anaemic erythrocytes. Significant changes are observed in anaemic cells as compared to normal cells. This article is protected by copyright. All rights reserved.
  • Development of multimodal micro-endoscopic system with oblique illumination for simultaneous fluorescence imaging and spectroscopy of oral cancer
    Pramila Thapa, Veena Singh, Sunil Bhatt, Shilpa Tayal, Priyanka Mann, et al.
    Journal of Biophotonics, 2022
    Multimodality of an optical system implies the use of one or more optical techniques to improve the system's overall performance and maximum utility. In this paper, we demonstrate a multi-modal system with oblique illumination that combines two different techniques; fluorescence micro-endoscopy and spectroscopy simultaneously and can be utilized to obtain diverse information from the same location of biological sample. In present system, use of graded-index (GRIN) rod-lens makes it highly compact and oblique incidence decouples illumination geometry with collection geometry, preventing CCD cameras from saturation and reduces number of optical elements, thereby making system further miniaturized and field-portable. It also overcomes disadvantages of undesired reflections from different optical elements. The experimental results of simultaneous imaging and spectroscopy of the biological samples are presented along with quantitative spectroscopic parameters; peak wavelength shift, area under the curve and full width half maximum (FWHM) are calculated. This article is protected by copyright. All rights reserved.
  • Multimodal biomicroscopic system for the characterization of cells with high spatial phase sensitivity and sub-pixel accuracy
    Shilpa Tayal, Veena Singh, Tejinder Kaur, Neetu Singh, Dalip Singh Mehta
    Journal of Biophotonics, 2022
    Multi-modal analysis is highly advantageous for various biomedical applications including cancer and brain studies. Simultaneous measurement of quantitative phase with sub-pixel accuracy and fluorescence image is difficult to achieve in single measurement. Conventionally, off- axis interferograms are analyzed using the Fourier-transform method which limits the accuracy of the phase maps by pixel size, and usually the location of the carrier peak is in sub-pixel. We report a multi-modal microscopic system consisting of high-resolution (HR) quantitative phase interferometer (QPI) to retrieve sub- pixel accuracy in phase imaging and an oblique-illumination based fluorescence imaging system which decouples the excited light from emitted signal light to avoid saturation of the camera, both integrated into a single unit. Here, highly-resolved phase maps are obtained using a two-step process. Firstly, using a speckle-free illumination which offers high spatial phase sensitivity. Secondly, using a hamming window for accurate estimation of original signal frequency information and HR discrete Fourier transform (DFT) which offers sub-pixel accuracy in phase measurements. HR-DFT has computational load of O(ABβ), where A×B is the size of the interferogram and β is the upsampling factor, making system computationally more robust and efficient compared to zero-padded FFT. The experiment is conducted on MG63 osteosarcoma and human mesenchymal stem cells( hMSCs) and their quantitative parameters are extracted with significantly improved accuracy. The average phase for MG63 cells and hMSCs; for nucleus is obtained to be 8.02rad ± 0.80rad and 4.29rad ± 0.43rad ,respectively and for cytoplasm is obtained to be 2.63rad ± 0.96rad and 1.73rad ± 0.57rad, respectively. This article is protected by copyright. All rights reserved.
  • Quantitative Phase Microscopy and Tomography: Techniques using partially spatially coherent monochromatic light
    Dalip Singh Mehta, Ankit Butola, Veena Singh
    Quantitative Phase Microscopy and Tomography Techniques Using Partially Spatially Coherent Monochromatic Light, 2022
  • Speckle noise reduction strategies in laser-based projection imaging, fluorescence microscopy, and digital holography with uniform illumination, improved image sharpness, and resolution
    Virendra Kumar, Atul Kumar Dubey, Mayank Gupta, Veena Singh, Ankit Butola, et al.
    Optics and Laser Technology, 2021
  • Monitoring the health of laser-driven phosphor-converted white light source using spectral interference technique
    Atul Kumar Dubey, Veena Singh, Mayank Gupta, Virendra Kumar, Dalip Singh Mehta
    Optics and Lasers in Engineering, 2021
  • Highly Stable, Field Portable, Single Shot and Low Cost LED Based Digital Holographic Microscope
    Veena Singh, Shilpa Tayal, Dalip Singh Mehta
    Springer Proceedings in Physics, 2021
  • White Light Interference Microscopy for Quantitative Phase Imaging of Biological Samples Using Hilbert Transform
    Priyanka Mann, Veena Singh, Shilpa Tayal, Vishesh Dubey, Dalip Singh Mehta
    Springer Proceedings in Physics, 2021
  • Development of multi-modal system for quantitative phase and fluorescence imaging of osteo cancer cells
    Optics Infobase Conference Papers, 2021
  • Development of Multi-Modal System for Quantitative Phase and Fluorescence Imaging of Osteo Cancer Cells
    Shilpa Tayal, Veena Singh, Tejinder Kaur, Neetu Singh, Dalip Singh Mehta
    Progress in Biomedical Optics and Imaging Proceedings of SPIE, 2021
  • White light interference microscopy and cell counting with wide field of view
    Priyanka Mann, Shilpa Tayal, Veena Singh, Pramila Thapa, Dalip Singh Mehta
    Progress in Biomedical Optics and Imaging Proceedings of SPIE, 2021
  • Field Portable Total Internal Reflection Fluorescence Microscopy
    Anuj Saxena, Vishesh Dubey, Veena Singh, Shilpa Tayal, Dalip Singh Mehta
    Springer Proceedings in Physics, 2021
  • White light interference microscopy and cell counting with wide field of view
    Optics Infobase Conference Papers, 2021
  • Field Portable Micro Endoscopy for In Vivo Microscopy of Cancerous Tissue: A Point of Care Device
    Pramila Thapa, Hansha Pandey, Veena Singh, Shilpa Tayal, Anurag Shrivastava, et al.
    Springer Proceedings in Physics, 2021
  • Field Portable Grin-Lens Based Micro-Endoscopic System with Oblique-Illumination for Cancer Screening
    Primala Thapa, Sunil Bhatt, Veena Singh, Shilpa Tayal, Priyanka Mann, et al.
    Progress in Biomedical Optics and Imaging Proceedings of SPIE, 2021
  • Field portable grin-lens based micro-endoscopic system with oblique-illumination for cancer screening
    Optics Infobase Conference Papers, 2021
  • Design and development of laser speckle reduction device using waveguide diffuser and pyramidal cavity for projection imaging
    Virendra Kumar, Mayank Gupta, Atul Kumar Dubey, Shilpa Tayal, Veena Singh, et al.
    Journal of Optics United Kingdom, 2020
  • Simultaneous fluorescence and quantitative phase imaging of MG63 osteosarcoma cells to monitor morphological changes with time using partially spatially coherent light source
    Shilpa Tayal, Veena Singh, Tejinder Kaur, Neetu Singh, Dalip Singh Mehta
    Methods and Applications in Fluorescence, 2020

RECENT SCHOLAR PUBLICATIONS

  • Experimental Investigation of Engine Performance for 2nd Generation Biodiesel Derived from Mg2Zr5O12 Catalyst
    V Singh, S Chavan, Y Sharma
    Energies 15 (11), 4044 , 2022
    2022
    Citations: 6
  • Scalable InkJet‐Based Additive Fabrication of Photocatalytic TiO 2 Thin Films
    V Singh, CM Lousada, M Jonsson, LM Belova
    ChemPhotoChem 6 (3), e202100212 , 2022
    2022
    Citations: 4
  • Biodiesel production using a novel heterogeneous catalyst, magnesium zirconate (Mg2Zr5O12): Process optimization through response surface methodology (RSM)
    V Singh, L Belova, B Singh, YC Sharma
    Energy Conversion and Management 174, 198-207 , 2018
    2018
    Citations: 134
  • Methyl transesterification of waste cooking oil using a laboratory synthesized reusable heterogeneous base catalyst: Process optimization and homogeneity study of catalyst
    M Yadav, V Singh, YC Sharma
    Energy Conversion and Management 148, 1438-1452 , 2017
    2017
    Citations: 99
  • Effect of co-solvent on biodiesel production using calcium aluminium oxide as a reusable catalyst and waste vegetable oil
    V Singh, M Yadav, YC Sharma
    Fuel 203, 360-369 , 2017
    2017
    Citations: 98
  • Production of biodiesel from three indigenous feedstock: Optimization of process parameters and assessment of various fuel properties
    SB Chavan, M Yadav, R Singh, V Singh, RR Kumbhar, YC Sharma
    Environmental Progress & Sustainable Energy 36 (3), 788-795 , 2017
    2017
    Citations: 41
  • Low cost guinea fowl bone derived recyclable heterogeneous catalyst for microwave assisted transesterification of Annona squamosa L. seed oil
    V Singh, YC Sharma
    Energy Conversion and Management 138, 627-637 , 2017
    2017
    Citations: 62
  • Synthesis of high-quality biodiesel using feedstock and catalyst derived from fish wastes
    D Madhu, R Arora, S Sahani, V Singh, YC Sharma
    Journal of Agricultural and Food Chemistry 65 (10), 2100-2109 , 2017
    2017
    Citations: 66
  • Synthesis of biodiesel from non-edible oil feedstocks using mixed metal oxides
    V Singh
    Indian Institute of Technology (Banaras Hindu University), Varanasi, India , 2017
    2017
  • Nano barium zirconate as heterogeneous catalyst for synthesis of biodiesel by transesterification of waste frying oil
    V Singh, Y Sharma
    20th CRSI-RSC National Symposium in Chemistry, Gauhati University, Guwahati … , 2017
    2017
  • Microalgal biodiesel: a possible solution for India’s energy security
    YC Sharma, V Singh
    Renewable and Sustainable Energy Reviews 67, 72-88 , 2017
    2017
    Citations: 118
  • Economically viable production of biodiesel from a rural feedstock from eastern India, P. pinnata oil using a recyclable laboratory synthesized heterogeneous catalyst
    V Singh, BH Hameed, YC Sharma
    Energy conversion and management 122, 52-62 , 2016
    2016
    Citations: 52
  • An economically viable synthesis of biodiesel from a crude Millettia pinnata oil of Jharkhand, India as feedstock and crab shell derived catalyst
    D Madhu, SB Chavan, V Singh, B Singh, YC Sharma
    Bioresource Technology 214, 210-217 , 2016
    2016
    Citations: 77
  • A low cost one pot synthesis of biodiesel from waste frying oil (WFO) using a novel material, β-potassium dizirconate (β-K2Zr2O5)
    V Singh, F Bux, YC Sharma
    Applied Energy 172, 23-33 , 2016
    2016
    Citations: 80
  • Biodiesel as a substantial source of renewable energy: Production and optimization by usingβ-potassium dizirconate (β-K2Zr2O5) as a heterogeneous catalyst
    V Singh, Y Sharma
    Second International Conference on Advanced Materials for Power Engineering … , 2016
    2016
  • Barium zirconate as a heterogeneous catalyst for production of biodiesel by jatropha: optimization of parameters
    V Singh, Y Sharma
    International Conference on Materials Science & Technology (ICMTech 2016 … , 2016
    2016
  • Synthesis of a novel heterogeneous catalyst β-Potassium dizirconate (β-K2Zr2O5) for biodiesel production
    V Singh, Y Sharma
    International conference on Multifunctional materials for Future … , 2015
    2015

MOST CITED SCHOLAR PUBLICATIONS

  • Biodiesel production using a novel heterogeneous catalyst, magnesium zirconate (Mg2Zr5O12): Process optimization through response surface methodology (RSM)
    V Singh, L Belova, B Singh, YC Sharma
    Energy Conversion and Management 174, 198-207 , 2018
    2018
    Citations: 134
  • Microalgal biodiesel: a possible solution for India’s energy security
    YC Sharma, V Singh
    Renewable and Sustainable Energy Reviews 67, 72-88 , 2017
    2017
    Citations: 118
  • Methyl transesterification of waste cooking oil using a laboratory synthesized reusable heterogeneous base catalyst: Process optimization and homogeneity study of catalyst
    M Yadav, V Singh, YC Sharma
    Energy Conversion and Management 148, 1438-1452 , 2017
    2017
    Citations: 99
  • Effect of co-solvent on biodiesel production using calcium aluminium oxide as a reusable catalyst and waste vegetable oil
    V Singh, M Yadav, YC Sharma
    Fuel 203, 360-369 , 2017
    2017
    Citations: 98
  • A low cost one pot synthesis of biodiesel from waste frying oil (WFO) using a novel material, β-potassium dizirconate (β-K2Zr2O5)
    V Singh, F Bux, YC Sharma
    Applied Energy 172, 23-33 , 2016
    2016
    Citations: 80
  • An economically viable synthesis of biodiesel from a crude Millettia pinnata oil of Jharkhand, India as feedstock and crab shell derived catalyst
    D Madhu, SB Chavan, V Singh, B Singh, YC Sharma
    Bioresource Technology 214, 210-217 , 2016
    2016
    Citations: 77
  • Synthesis of high-quality biodiesel using feedstock and catalyst derived from fish wastes
    D Madhu, R Arora, S Sahani, V Singh, YC Sharma
    Journal of Agricultural and Food Chemistry 65 (10), 2100-2109 , 2017
    2017
    Citations: 66
  • Low cost guinea fowl bone derived recyclable heterogeneous catalyst for microwave assisted transesterification of Annona squamosa L. seed oil
    V Singh, YC Sharma
    Energy Conversion and Management 138, 627-637 , 2017
    2017
    Citations: 62
  • Economically viable production of biodiesel from a rural feedstock from eastern India, P. pinnata oil using a recyclable laboratory synthesized heterogeneous catalyst
    V Singh, BH Hameed, YC Sharma
    Energy conversion and management 122, 52-62 , 2016
    2016
    Citations: 52
  • Production of biodiesel from three indigenous feedstock: Optimization of process parameters and assessment of various fuel properties
    SB Chavan, M Yadav, R Singh, V Singh, RR Kumbhar, YC Sharma
    Environmental Progress & Sustainable Energy 36 (3), 788-795 , 2017
    2017
    Citations: 41
  • Experimental Investigation of Engine Performance for 2nd Generation Biodiesel Derived from Mg2Zr5O12 Catalyst
    V Singh, S Chavan, Y Sharma
    Energies 15 (11), 4044 , 2022
    2022
    Citations: 6
  • Scalable InkJet‐Based Additive Fabrication of Photocatalytic TiO 2 Thin Films
    V Singh, CM Lousada, M Jonsson, LM Belova
    ChemPhotoChem 6 (3), e202100212 , 2022
    2022
    Citations: 4
  • Synthesis of biodiesel from non-edible oil feedstocks using mixed metal oxides
    V Singh
    Indian Institute of Technology (Banaras Hindu University), Varanasi, India , 2017
    2017
  • Nano barium zirconate as heterogeneous catalyst for synthesis of biodiesel by transesterification of waste frying oil
    V Singh, Y Sharma
    20th CRSI-RSC National Symposium in Chemistry, Gauhati University, Guwahati … , 2017
    2017
  • Biodiesel as a substantial source of renewable energy: Production and optimization by usingβ-potassium dizirconate (β-K2Zr2O5) as a heterogeneous catalyst
    V Singh, Y Sharma
    Second International Conference on Advanced Materials for Power Engineering … , 2016
    2016
  • Barium zirconate as a heterogeneous catalyst for production of biodiesel by jatropha: optimization of parameters
    V Singh, Y Sharma
    International Conference on Materials Science & Technology (ICMTech 2016 … , 2016
    2016
  • Synthesis of a novel heterogeneous catalyst β-Potassium dizirconate (β-K2Zr2O5) for biodiesel production
    V Singh, Y Sharma
    International conference on Multifunctional materials for Future … , 2015
    2015