Veena Awadhesh Singh
@kth.se
Postdoctoral Fellow, Department of material Science and Engineering
KTH Royal Institute of Technology, Stockholm, Sweden
EDUCATION
PhD. in Chemistry (2018) Indian Institute of Technology Banaras Hindu University, Varanasi, India
MSc: (2012) Organic Chemistry, University of Pune, Maharashtra, India
BSc: (2010) Chemistry, University of Pune, Maharashtra, India
RESEARCH INTERESTS
Material synthesis, Energy storage, Battery and Supercapacitor, Thin film deposition, Inkjet technology, Magnetic sputtering, Additive manufacturing, Photocatalysis, Solar cells, Renewable energy resources execution, Biofuels, Biodiesel production
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Dalip Singh Mehta, Pramila Thapa, Veena Singh, Himanshu Joshi, Dibya Jyoti Sarangi, Deepika Mishra, and Anurag Srivastava
Elsevier BV
Pramila Thapa, Veena Singh, Sunil Bhatt, Kiran Maurya, Virendra Kumar, Vivek Nayyar, Kiran Jot, Deepika Mishra, Anurag Shrivastava, and Dalip Singh Mehta
IOP Publishing
Abstract The survival rate of oral squamous cell carcinoma (OSCC) patients is very poor, but it can be improved using highly sensitive, specific, and accurate techniques. Autofluorescence and fluorescence techniques are very sensitive and helpful in cancer screening; being directly linked with the molecular levels of human tissue, they can be used as a quantitative tool for cancer detection. Here, we report the development of multi-modal autofluorescence and fluorescence imaging and spectroscopic (MAF-IS) smartphone-based systems for fast and real-time oral cancer screening. MAF-IS system is indigenously developed and offers the advantages of being a low-cost, handy, non-contact, non-invasive, and easily operable device that can be employed in hospitals, including low-resource settings. In this study, we report the results of 43 individuals with 28 OSCC and 15 oral potentially malignant disorders (OPMDs), i.e., epithelial dysplasia and oral submucous fibrosis, using the developed devices. We observed a red shift in fluorescence emission spectra in vivo. We found red-shift of 7.72 ± 6 nm, 3 ± 4.36 nm, and 1.33 ± 0.47 nm in the case of OSCC, epithelial dysplasia, and oral submucous fibrosis, respectively, compared to normal. The results were compared with histopathology and found to be consistent. Further, the MAF-IS system provides results in real-time with higher accuracy and sensitivity compared to devices using a single modality. Our system can achieve an accuracy of 97% with sensitivity and specificity of 100% and 94.7%, respectively, even with a smaller number of patients (28 patients of OSCC). The proposed MAF-IS device has great potential for fast screening and diagnosis of oral cancer in the future.
Pramila Thapa, Veena Singh, Komal Gupta, Anurag Shrivastava, Virendra Kumar, Kamal Kataria, Piyush R. Mishra, and Dalip S. Mehta
Wiley
OBJECTIVE
Fluorescence-based methods are highly specific and sensitive and have potential in breast cancer detection. Simultaneous fluorescence imaging and spectroscopy during intraoperative procedures of breast cancer have great advantages in detection of tumor margin as well as in classification of tumor to healthy tissues. Intra-operative real-time confirmation of breast cancer tumor margin is the aim of surgeons, and therefore, there is an urgent need for such techniques and devices which fulfill the surgeon's priorities.
METHODS
In this article, we propose the development of fluorescence-based smartphone imaging and spectroscopic point-of-care multi-modal devices for detection of invasive ductal carcinoma in tumor margin during removal of tumor. These multimodal devices are portable, cost-effective, noninvasive, and user-friendly. Molecular level sensitivity of fluorescence process shows different behavior in normal, cancerous and marginal tissues. We observed significant spectral changes, such as, red-shift, full-width half maximum (FWHM), and increased intensity as we go towards tumor center from normal tissue. High contrast in fluorescence images and spectra are also recorded for cancer tissues compared to healthy tissues. Preliminary results for the initial trial of the devices are reported in this article.
RESULTS
A total 44 spectra from 11 patients of invasive ductal carcinoma (11 spectra for invasive ductal carcinoma and rest are normal and negative margins) are used. Principle component analysis is used for the classification of invasive ductal carcinoma with an accuracy of 93%, specificity of 75% and sensitivity of 92.8%. We obtained an average 6.17 ± 1.66 nm red shift for IDC with respect to normal tissue. The red shift and maximum fluorescence intensity indicates p < 0.01. These results described here are supported by histopathological examination of the same sample.
CONCLUSION
In the present manuscript, simultaneous fluorescence-based imaging and spectroscopy is accomplished for the classification of IDC tissues and breast cancer margin detection.
Veena Singh, Sudhanshu Kuthe, and Natalia V. Skorodumova
MDPI AG
Development of reliable energy storage technologies is the key for the consistent energy supply based on alternate energy sources. Among energy storage systems, the electrochemical storage devices are the most robust. Consistent energy storage systems such as lithium ion (Li ion) based energy storage has become an ultimate system utilized for both domestic and industrial scales due to its advantages over the other energy storage systems. Considering the factors related to Li ion-based energy storage system, in the present review, we discuss various electrode fabrication techniques including electrodeposition, chemical vapor deposition (CVD), stereolithography, pressing, roll to roll, dip coating, doctor blade, drop casting, nanorod growing, brush coating, stamping, inkjet printing (IJP), fused deposition modelling (FDM) and direct ink writing (DIW). Additionally, we analyze the statistics of publications on these fabrication techniques and outline challenges and future prospects for the Li ion battery market.
Veena Singh and Lyubov Belova
MDPI AG
We report the fabrication and characterization of solution-route CeO2 thin films with a tunable porosity and microstructure. Films were deposited by means of inkjet printing technique using 0.2 M, 0.4 M and 0.6 M concentration inks prepared from Ce(NO3)3·6H2O precursor. Printing was performed at two different temperatures of 60 °C and 300 °C to study the variation in structure. Printing parameters were adjusted for the consecutive deposition of layers, resulting in ≈140 nm and ≈185 nm thick single layers for 60 °C and 300 °C printing temperatures, respectively. We compared the microstructure of printed films for different concentrations, printing temperatures, solvents and substrates. The formation of the cubic fluorite structure of the printed films was confirmed via XRD characterization. We suggest this technique as an advanced method for high-quality film fabrication with a controlled microstructure and with a minimal waste of materials. Through adjusting printing parameters, both dense and porous films can be produced for use in different applications.
Pramila Thapa, Veena Singh, Komal Gupta, Anurag Srivastava, and Dalip S. Mehta
SPIE
Breast cancer is most common cancer among women world-wide and can be treated if diagnosed at early-stage. Fluorescence (FL) techniques have a considerable impact in tumor detection as FL is highly sensitive to biochemical and biophysical structure of the tissues, providing novel techniques for early and noninvasive diagnosis of cancer. Due to high sensitivity of FL, it can be used in early-stage breast cancer detection. Fluorescence-guided-surgery (FGS) using exogenous agent in breast cancer is a well-known method, which locates tumor and margins during intra-operative procedure. FL-spectroscopy and FL-imaging has shown the potentiality in FGS of breast cancer independently. Combination of these two modalities in a single system improves the success rate of FGS which can give tumor free tissues. The goal of the current study is to analyze and classify the early-stage breast cancer, locally advanced breast cancer (invasive ductal carcinoma) and normal tissue. For that, we combine FL-imaging and FL-spectroscopy in a single smartphone-based point-of-care devices and recorded data during intra-operative procedure. A total 21 patients of invasive ductal carcinoma and fibroadenoma are included in this study. Total 65 FL-spectra are recorded during intra-operative procedure which are further used in support vector machine (SVM) based classification of fibroadenoma, invasive ductal carcinoma (IDC) and normal tissue. The sensitivity, specificity, positive predictive value, negative predictive value, and overall diagnostic accuracy (total efficiency) is 78.6 %, 90%, 91.6%, 75%, 95%, respectively. Additionally, we observed a red shift in case of IDC and fibroadenoma from normal tissue, which is 5.22±1.77 nm, and 4.96±2.61 nm, respectively.
Shilpa Tayal, Anuj Saxena, Veena Singh, Tejinder Kaur, Neetu Singh, Kedar Khare, and Dalip Singh Mehta
Elsevier BV
Priyanka Mann, Veena Singh, Shilpa Tayal, Pramila Thapa, and Dalip Singh Mehta
Wiley
In this paper, we demonstrate the white light phase shifting Interferometer employed as whole slide scanner and phase profiler for determining qualitative and quantitative information over large field-of-view. Experiments were performed on human erythrocytes and MG63 Osteosarcoma cells. Here, we have recorded microscopic images and phase shifted white light interferograms simultaneously in a stepped manner. Sample slide is translated in transverse direction such that there exists a correlation between the adjacent frames, and they were stitched together using correlation functions. Final stitched image has a field-of-view of 0.24*1.14 mm with high resolution ~0.8 μm. Circular Hough transform algorithm is implemented to the resulting image for cell counting and five-step phase shifting algorithm is utilised to retrieve the phase profiles over a large field-of-view. Further, this technique is utilised to study the difference between normal and anaemic erythrocytes. Significant changes are observed in anaemic cells as compared to normal cells. This article is protected by copyright. All rights reserved.
Veena Singh, Supriya B. Chavan, and Yogesh C. Sharma
MDPI AG
In the present study, experimental analyses were conducted by using biodiesel derived from second-generation feedstock. In terms of cost and accessibility, second-generation feedstock has gained more attention due to its environmental approach. Waste-cooking-oil-derived methyl ester was produced through a transesterification reaction in the presence of a synthesized magnesium zirconate (Mg2Zr5O12) heterogeneous catalyst. This trans-esterified waste cooking oil (WCO) was used as biodiesel and was blended with diesel in 10%, 20%, 30%, 40%, and 50% by volume ratio for further analysis. The fuel properties of pure and blended biodiesel were investigated in terms of flash point, density, kinematic viscosity, and lower heating value as per the American Society for Testing and Materials (ASTM) D-6751 standards. For each blended fuel, the engine performance and gaseous emissions trend with engine loads of 0, 3, 6, 9, and 12 kg were measured on a Kirloskar TV1 IC engine. The results indicated that the 40% blended biodiesel has the maximum brake thermal efficiency (BTE) of 19.13% and exhaust gas temperature (EGT) of 6.98% increment, also showing an increase with respect to engine load. On the other hand, brake-specific fuel consumption (BSFC) was highest for 40% blending as 36.48% increase, and that decreases with the increase in engine loads. Significant reductions in carbon monoxide (CO) and unburned hydrocarbon (HC) emissions were observed for 40% blended fuel and were 34.78% and 38.1% reduction, respectively. CO and HC emissions decreased with respect to the engine load. Meanwhile, reverse trends for carbon dioxide (CO2) and nitrogen oxide (NOx) have been observed as 14.57% and 27.85% increases for 100% biodiesel. CO2 and NOx increased with increase in engine load. The mass balance and environmental factor of crude and purified biodiesel were studied to show the environmental suitability of synthesized product. Overall, the results showed that the blended biodiesel can be used as a substitute and has an advantage over diesel fuel. The main contribution derived from this work is to improve engine performance and gaseous emission by using blended biodiesel derived from a recyclable heterogeneous catalyst and waste-cooking-oil feedstock.
Pramila Thapa, Veena Singh, Sunil Bhatt, Shilpa Tayal, Priyanka Mann, Kiran Maurya, Deepika Mishra, and Dalip Singh Mehta
Wiley
Multimodality of an optical system implies the use of one or more optical techniques to improve the system's overall performance and maximum utility. In this paper, we demonstrate a multi-modal system with oblique illumination that combines two different techniques; fluorescence micro-endoscopy and spectroscopy simultaneously and can be utilized to obtain diverse information from the same location of biological sample. In present system, use of graded-index (GRIN) rod-lens makes it highly compact and oblique incidence decouples illumination geometry with collection geometry, preventing CCD cameras from saturation and reduces number of optical elements, thereby making system further miniaturized and field-portable. It also overcomes disadvantages of undesired reflections from different optical elements. The experimental results of simultaneous imaging and spectroscopy of the biological samples are presented along with quantitative spectroscopic parameters; peak wavelength shift, area under the curve and full width half maximum (FWHM) are calculated. This article is protected by copyright. All rights reserved.
Shilpa Tayal, Veena Singh, Tejinder Kaur, Neetu Singh, and Dalip Singh Mehta
Wiley
Multi-modal analysis is highly advantageous for various biomedical applications including cancer and brain studies. Simultaneous measurement of quantitative phase with sub-pixel accuracy and fluorescence image is difficult to achieve in single measurement. Conventionally, off- axis interferograms are analyzed using the Fourier-transform method which limits the accuracy of the phase maps by pixel size, and usually the location of the carrier peak is in sub-pixel. We report a multi-modal microscopic system consisting of high-resolution (HR) quantitative phase interferometer (QPI) to retrieve sub- pixel accuracy in phase imaging and an oblique-illumination based fluorescence imaging system which decouples the excited light from emitted signal light to avoid saturation of the camera, both integrated into a single unit. Here, highly-resolved phase maps are obtained using a two-step process. Firstly, using a speckle-free illumination which offers high spatial phase sensitivity. Secondly, using a hamming window for accurate estimation of original signal frequency information and HR discrete Fourier transform (DFT) which offers sub-pixel accuracy in phase measurements. HR-DFT has computational load of O(ABβ), where A×B is the size of the interferogram and β is the upsampling factor, making system computationally more robust and efficient compared to zero-padded FFT. The experiment is conducted on MG63 osteosarcoma and human mesenchymal stem cells( hMSCs) and their quantitative parameters are extracted with significantly improved accuracy. The average phase for MG63 cells and hMSCs; for nucleus is obtained to be 8.02rad ± 0.80rad and 4.29rad ± 0.43rad ,respectively and for cytoplasm is obtained to be 2.63rad ± 0.96rad and 1.73rad ± 0.57rad, respectively. This article is protected by copyright. All rights reserved.
Veena Singh, Cláudio M. Lousada, Mats Jonsson, and Liubov M. Belova
Wiley
Dalip Singh Mehta, Ankit Butola, and Veena Singh
IOP Publishing
Virendra Kumar, Atul Kumar Dubey, Mayank Gupta, Veena Singh, Ankit Butola, and Dalip Singh Mehta
Elsevier BV
Atul Kumar Dubey, Veena Singh, Mayank Gupta, Virendra Kumar, and Dalip Singh Mehta
Elsevier BV
Shilpa Tayal, Veena Singh, Tejinder Kaur, Neetu Singh, and Dalip Singh Mehta
SPIE
Primala Thapa, Sunil Bhatt, Veena Singh, Shilpa Tayal, Priyanka Mann, Anurag Shrivastava, and Dalip Singh Mehta
SPIE
We describe field-portable GRIN lens based micro-endoscope with oblique-illumination for cancer screening. Fluorescence microscopic images of different samples were recorded with micro-endoscope which provides molecular information about the sample.
Priyanka Mann, Shilpa Tayal, Veena Singh, Pramila Thapa, and Dalip Singh Mehta
SPIE
We report classification between normal and anemic erythrocytes by determining cell counts computationally using Circular Hough transform algorithm in matlab and quantifying phase map which are important for early diagnosis of diseases.
Veena Singh, Shilpa Tayal, and Dalip Singh Mehta
Springer Singapore
Pramila Thapa, Hansha Pandey, Veena Singh, Shilpa Tayal, Anurag Shrivastava, and D. S. Mehta
Springer Singapore
Priyanka Mann, Veena Singh, Shilpa Tayal, Vishesh Dubey, and Dalip Singh Mehta
Springer Singapore
Anuj Saxena, Vishesh Dubey, Veena Singh, Shilpa Tayal, and Dalip Singh Mehta
Springer Singapore