Education, Molecular Biology, Health Professions, Multidisciplinary
29
Scopus Publications
Scopus Publications
Consolidation of a genomic epidemiological surveillance network for tuberculosis (REVIGET) in northern and northeastern Brazil: a study protocol Emilyn Costa Conceição, Karla Valéria Batista Lima, Cristiane Cunha Frota, Theolis Costa Barbosa Bessa, Adriana Ayden Ferreira, Abhinav Sharma, Davi Josué Marcon, Layana Rufino Ribeiro, Alex Brito Souza, Danna Karen Corrêa dos Santos, Carlos Augusto Abreu Alberio, Ricardo José de Paula Souza e Guimarães, Ismari Perini Furlaneto, Lilian Eduarda de Oliveira Freitas, Emmily Oliveira Amador, Hendor Neves Ribeiro de Jesus, Leonardo Bruno Paz Ferreira Barreto, Maria Cristina Silva Lourenço, Tulio de Oliveira, Robin Mark Warren, and Frontiers in Public Health, 2025 Globally, tuberculosis (TB) remains a top cause of death from infectious diseases, with an estimated 1.5 million deaths annually. Given its substantial social and economic burden, TB is a priority in the United Nations 2030 Agenda for Sustainable Development. The WHO’s End TB Strategy emphasizes research, innovation, and the rapid implementation of new technologies, such as whole-genome sequencing (WGS), which are pivotal for precision health approaches and drug-resistant TB (DR-TB) surveillance. This study aims to strengthen genomic TB surveillance in the North and Northeast Brazilian regions by applying WGS to study DR-TB cases, training professionals in genomics and bioinformatics, and deploying a national surveillance platform (GEMIBRA). This is an observational, cross-sectional, prospective, quantitative, and qualitative study to be conducted in the states of Pará, Amazonas, Ceará, and Bahia. A total of 500 Mycobacterium tuberculosis complex (MTBC) isolates from DR-TB cases will undergo whole-genome sequencing (WGS), representing 87% of the expected DR-TB cases, including non-tuberculous mycobacteria (NTM) isolates among the DR samples. Data will be analyzed for genotype–phenotype correlations, mutation patterns, and associations with clinical and epidemiological characteristics. Capacity-building activities, including theoretical and hands-on bioinformatics training, will be carried out. The GEMIBRA platform will support data visualization, spatial distribution, and clinical decision-making. The project will generate evidence to validate the contribution of WGS integration in Brazil’s TB surveillance system, support precision treatment approaches, and inform public health interventions. It will also contribute to workforce development and the introduction of decentralized WGS-based diagnostics in resource-limited regions. The project adopts a translational research model and a networked, decentralized approach, facilitating the prompt integration of the knowledge generated into public health practice. Ultimately, this work will contribute to combating TB transmission by identifying transmission dynamics, emerging resistant strains, and informing the National Plan to End TB as a public health problem.
The Role of GeneXpert® for Tuberculosis Diagnostics in Brazil: An Examination from a Historical and Epidemiological Perspective Tirça Naiara da Silva Iúdice, Marília Lima da Conceição, Artemir Coelho de Brito, Nicole Menezes de Souza, Cristal Ribeiro Mesquita, Ricardo José de Paula Souza e Guimarães, Ismari Perini Furlaneto, Alessandra de Souza Saboia, Maria Cristina da Silva Lourenço, Karla Valéria Batista Lima, Emilyn Costa Conceição Tropical Medicine and Infectious Disease, 2023 The rapid molecular test (RMT) performed on the GeneXpert® system is widely used as a control strategy and surveillance technique for tuberculosis (TB). In the region of the Americas, TB incidence is slowly increasing owing to an upward trend in Brazil, which is among the high TB-burden countries (HBCs), ranking in the 19th position. In this context, we aimed to (i) describe the implementation and history of RMT-TB (Xpert® MTB/RIF and Xpert® MTB/RIF Ultra) in Brazil; (ii) to evaluate the national RMT laboratory distribution, TB, and resistance to RIF detection by RMT; and (iii) to correlate these data with Brazilian TB incidence. The quantitative data of Xpert® MTB/RIF and Xpert® MTB/RIF Ultra assays performed in the pulmonary TB investigation from 2014 to 2020 were provided by the Brazilian Ministry of Health. A spatial visualization using ArcGIS software was performed. The Southeast region constituted about half of the RMT laboratories—from 39.4% to 45.9% of the total value over the five regions. Regarding the federal units, the São Paulo state alone represented from 20.2% to 34.1% (5.0 to 8.5 times the value) of RMT laboratories over the years observed. There were significant differences (p < 0.0001) in the frequency of RMT laboratories between all years of the historical series. There was an unequal distribution of RMT laboratories between Brazilian regions and federal units. This alerts us for the surveillance of rapid molecular detection of TB in different parts of the country, with the possibility of improving the distribution of tests in areas of higher incidence in order to achieve the level of disease control recommended by national and worldwide authorities.
Comparative Analysis of Human Hepatic Lesions in Dengue, Yellow Fever, and Chikungunya: Revisiting Histopathological Changes in the Light of Modern Knowledge of Cell Pathology Yasmin Pacheco Ribeiro, Luiz Fabio Magno Falcão, Vanessa Cavaleiro Smith, Jorge Rodrigues de Sousa, Carla Pagliari, Edna Cristina Santos Franco, Ana Cecília Ribeiro Cruz, Janniffer Oliveira Chiang, Livia Carício Martins, Juliana Abreu Lima Nunes, Fellipe Souza da Silva Vilacoert, Lais Carneiro dos Santos, Matheus Perini Furlaneto, Hellen Thais Fuzii, Marcos Virgilio Bertonsin Filho, Luccas Delgado da Costa, Maria Irma Seixas Duarte, Ismari Perini Furlaneto, Arnaldo Jorge Martins Filho, Tinara Leila de Souza Aarão, Pedro Fernando da Costa Vasconcelos, Juarez Antônio Simões Quaresma Pathogens, 2023 Arboviruses, such as yellow fever virus (YFV), dengue virus (DENV), and chikungunya virus (CHIKV), present wide global dissemination and a pathogenic profile developed in infected individuals, from non-specific clinical conditions to severe forms, characterised by the promotion of significant lesions in different organs of the harbourer, culminating in multiple organ dysfunction. An analytical cross-sectional study was carried out via the histopathological analysis of 70 samples of liver patients, collected between 2000 and 2017, with confirmed laboratory diagnoses, who died due to infection and complications due to yellow fever (YF), dengue fever (DF), and chikungunya fever (CF), to characterise, quantify, and compare the patterns of histopathological alterations in the liver between the samples. Of the histopathological findings in the human liver samples, there was a significant difference between the control and infection groups, with a predominance of alterations in the midzonal area of the three cases analysed. Hepatic involvement in cases of YF showed a greater intensity of histopathological changes. Among the alterations evaluated, cell swelling, microvesicular steatosis, and apoptosis were classified according to the degree of tissue damage from severe to very severe. Pathological abnormalities associated with YFV, DENV, and CHIKV infections showed a predominance of changes in the midzonal area. We also noted that, among the arboviruses studied, liver involvement in cases of YFV infection was more intense.
Different cell death mechanisms are involved in leprosy pathogenesis Jorge Rodrigues de Sousa, Luiz Fábio Magno Falcão, Gabriela Lobato Virgolino, Matheus Ferreira Santos Cruz, Vinicius Fialho Teixeira, Tinara Leila de Souza Aarão, Ismari Perinii Furlaneto, Francisca Regina Oliveira Carneiro, Gabriela Amin, Hellen Thais Fuzii, Juarez Antonio Simões Quaresma Microbial Pathogenesis, 2022
Editorial Comment: Image-guided study of swine anatomy as a tool for urologic surgery research and training Luciano A. Favorito International Braz J Urol, 2021 1 Graduate student, School of Medicine, Centro Universitário do Estado do Pará, Belém-PA, Brazil; 2 MSc, Associate Professor, Department of Urology, School of Medicine, Centro Universitário do Estado do Pará, Belém-PA, Brazil; 3 PhD, Grupo de Pesquisa Experimental, Centro Universitário do Estado do Pará, Belém-PA, Brazil; 4 MSc, Associate Professor, Department of Radiology, School of Medicine, Centro Universitário do Estado do Pará, Belém-PA, Brazil
Association of the polymorphism of the vitamin D receptor gene (VDR) with the risk of leprosy in the Brazilian Amazon Jasna Letícia Pinto Paz, Maria do Perpétuo Socorro Corrêa Amador Silvestre, Letícia Siqueira Moura, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Karla Valéria Batista Lima, Luana Nepomuceno Gondim Costa Lima Bioscience Reports, 2021 The transmission and evolution of leprosy depends on several aspects, including immunological and genetic factors of the host, as well as genetic factors of Mycobacterium leprae. The present study evaluated the association of single nucleotide polymorphisms (SNPs) on the FokI (rs2228570), TaqI (rs731236), ApaI (rs7975232) regions of the vitamin D receptor (VDR) gene with leprosy. A total of 405 individuals were evaluated, composed by groups of 100 multibacillary (MB) and 57 paucibacillary (PB) patients, and 248 healthy contacts. Blood samples were collected from patients and contacts. The genotyping was performed by sequencing of the interest regions. The alleles of the studied SNPs, and SNP FokI genotypes, were not associated with leprosy. For the SNP on TaqI region, the relationship between the tt genotype, and for the SNP ApaI, the AA genotype, revealed an association with susceptibility to MB form, while Aa genotype with protection. The extended genotypes AaTT and AaTt of ApaI and TaqI were associated with protection against MB form. Further studies analyzing the expression of the VDR gene and the correlation with its SNPs might help to clarify the role of polymorphisms on the immune response in leprosy.
Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon Silvia Cristina Da Silva Pedroso, Karla Valéria Batista Lima, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Darlene Kássia Saraiva Queiroz Pantoja, Alex Junior Souza de Souza, Washington Luiz Assunção Pereira Journal of Infection in Developing Countries, 2021 Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region.
 Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively.
 Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.
 Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil.
Surgical site infection following caesarean section by acinetobacter species: A report from a hyperendemic setting in the brazilian amazon region Blenda Gonçalves Cabral, Danielle Murici Brasiliense, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Karla Valéria Batista Lima Microorganisms, 2021 Surgical site infection (SSI) following caesarean section is associated with increased morbidity, mortality, and significant health care costs. This study evaluated the epidemiological, clinical, and microbiological features of Acinetobacter spp. in women with SSIs who have undergone caesarean section at a referral hospital in the Brazilian Amazon region. This study included 69 women with post-caesarean SSI by Acinetobacter spp. admitted to the hospital between January 2012 and May 2015. The 69 Acinetobacter isolates were subjected to molecular species identification, antimicrobial susceptibility testing, detection of carbapenemase-encoding genes, and genotyping. The main complications of post-caesarean SSI by Acinetobacter were inadequate and prolonged antibiotic therapy, sepsis, prolonged hospitalization, and re-suture procedures. A. baumannii, A. nosocomialis and A. colistiniresistens species were identified among the isolates. Carbapenem resistance was associated with OXA-23-producing A. baumannii isolates and IMP-1-producing A. nosocomialis isolate. Patients with multidrug-resistant A. baumannii infection showed worse clinical courses. Dissemination of persistent epidemic clones was observed, and the main clonal complexes (CC) for A. baumannii were CC231 and CC236 (Oxford scheme) and CC1 and CC15 (Pasteur scheme). This is the first report of a long-term Acinetobacter spp. outbreak in women who underwent caesarean section at a Brazilian hospital. This study demonstrates the impact of multidrug resistance on the clinical course of post-caesarean infections.
Comparative angiotomographic study of swine vascular anatomy: Contributions to research and training models in vascular and endovascular surgery Adenauer Marinho de Oliveira Góes Junior, Rosa Helena de Figueiredo Chaves, Ismari Perini Furlaneto, Emanuelle de Matos Rodrigues, Flávia Beatriz Araújo de Albuquerque, Jacob Hindrik Antunes Smit, Carolina Pinheiro de Oliveira, Simone de Campos Vieira Abib Jornal Vascular Brasileiro, 2021 Resumo Contexto Modelos com animais de médio e grande porte permitem que pesquisadores avaliem a eficácia e a segurança de procedimentos cardiovasculares em sistemas que se assemelham à anatomia humana e podem ser usados para simular cenários para fins de treinamento. Embora modelos suínos tenham sido extensivamente utilizados, muitos fatores fisiológicos e anatômicos permanecem desconhecidos ou apenas superficialmente descritos. Objetivos Descrever a anatomia vascular do suíno por tomografia computadorizada, compará-la à anatomia humana e discutir a aplicação dos modelos porcinos em procedimentos abertos e endovasculares. Métodos Três porcos machos da raça Landrace foram submetidos a tomografia computadorizada. A anatomia vascular de pescoço, tórax, abdome e membros foi analisada e descrita; foram destacadas similaridades e divergências relevantes entre a anatomia vascular de suínos e de humanos e as implicações em procedimentos vasculares nos suínos. Resultados O território carotídeo, o arco aórtico e os ramos terminais da aorta em suínos apresentaram diferenças marcantes quando comparados aos de humanos. Foram detectadas compressões de veias renal e ilíaca comum, ambas à esquerda, semelhantes às encontradas nas síndromes humanas de Nutcracker e May-Thurner. Medidas vasculares (diâmetro, comprimento e ângulos) de diferentes topografias de suínos foram fornecidas. Conclusões Os dados fornecidos podem ser úteis para o planejamento de ensaios pré-clínicos e pesquisa básica, bem como para o refinamento do treinamento cirúrgico usando modelos suínos no campo da cirurgia vascular.
Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: A literature review Emilyn Costa Conceição, Richard Steiner Salvato, Karen Machado Gomes, Arthur Emil dos Santos Guimarães, Marília Lima da Conceição, Ricardo José de Paula Souza e Guimarães, Abhinav Sharma, Ismari Perini Furlaneto, Regina Bones Barcellos, Valdes Roberto Bollela, Lívia Maria Pala Anselmo, Maria Carolina Sisco, Cristina Viana Niero, Lucilaine Ferrazoli, Guislaine Refrégier, Maria Cristina da Silva Lourenço, Harrison Magdinier Gomes, Artemir Coelho de Brito, Marcos Catanho, Rafael Silva Duarte, Philip Noel Suffys, Karla Valéria Batista Lima Memorias do Instituto Oswaldo Cruz, 2021
Influence of tramadol on functional recovery of acute spinal cord injury in rats Rosa Helena de Figueiredo Chaves, Celice Cordeiro de Souza, Ismari Perini Furlaneto, Renan Kleber Costa Teixeira, Carolina Pinheiro de Oliveira, Emanuelle de Matos Rodrigues, Daniel Arthur Santos dos Santos, Renata Cunha Silva, Nelson Elias Abrahão da Penha, Ana Rita de Lima Acta Cirurgica Brasileira, 2018
Analysis of potential household transmission events of tuberculosis in the city of Belem, Brazil Emilyn Costa Conceição, Arthur Emil dos Santos Guimarães, Maria Luíza Lopes, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Marília Lima da Conceição, Wandyra Araújo Barros, Ninarosa Calzavara Cardoso, Abhinav Sharma, Luana Nepomuceno Gondim Costa Lima, Harrison Magdinier Gomes, Rafael Silva Duarte, Cristiane Frota, Liliana K. Rutaihwa, Sebastien Gagneux, Philip Noel Suffys, Karla Valéria Batista Lima Tuberculosis, 2018
The effect of microneedling on scars resulting from induced cutaneous injuries in rats Andressa Miléo Ferraioli Silva, Wanessa Cardoso Praia, Caroline da Silva Alves Palheta, Rodrigo Paracampo Couteiro, Andrew Moraes Monteiro, Luciana Mota Silva, Ismari Perini Furlaneto, Josie Eiras Bisi dos Santos, Miguel Saraty de Oliveira Surgical and Cosmetic Dermatology, 2018
