Ismari Perini Furlaneto
@cesupa.br
curso de medicina
CESUPA
RESEARCH, TEACHING, or OTHER INTERESTS
Education, Molecular Biology, Health Professions, Multidisciplinary
Scopus Publications
Scopus Publications
Tirça Naiara da Silva Iúdice, Marília Lima da Conceição, Artemir Coelho de Brito, Nicole Menezes de Souza, Cristal Ribeiro Mesquita, Ricardo José de Paula Souza e Guimarães, Ismari Perini Furlaneto, Alessandra de Souza Saboia, Maria Cristina da Silva Lourenço, Karla Valéria Batista Lima,et al.
MDPI AG
The rapid molecular test (RMT) performed on the GeneXpert® system is widely used as a control strategy and surveillance technique for tuberculosis (TB). In the region of the Americas, TB incidence is slowly increasing owing to an upward trend in Brazil, which is among the high TB-burden countries (HBCs), ranking in the 19th position. In this context, we aimed to (i) describe the implementation and history of RMT-TB (Xpert® MTB/RIF and Xpert® MTB/RIF Ultra) in Brazil; (ii) to evaluate the national RMT laboratory distribution, TB, and resistance to RIF detection by RMT; and (iii) to correlate these data with Brazilian TB incidence. The quantitative data of Xpert® MTB/RIF and Xpert® MTB/RIF Ultra assays performed in the pulmonary TB investigation from 2014 to 2020 were provided by the Brazilian Ministry of Health. A spatial visualization using ArcGIS software was performed. The Southeast region constituted about half of the RMT laboratories—from 39.4% to 45.9% of the total value over the five regions. Regarding the federal units, the São Paulo state alone represented from 20.2% to 34.1% (5.0 to 8.5 times the value) of RMT laboratories over the years observed. There were significant differences (p < 0.0001) in the frequency of RMT laboratories between all years of the historical series. There was an unequal distribution of RMT laboratories between Brazilian regions and federal units. This alerts us for the surveillance of rapid molecular detection of TB in different parts of the country, with the possibility of improving the distribution of tests in areas of higher incidence in order to achieve the level of disease control recommended by national and worldwide authorities.
Yasmin Pacheco Ribeiro, Luiz Fabio Magno Falcão, Vanessa Cavaleiro Smith, Jorge Rodrigues de Sousa, Carla Pagliari, Edna Cristina Santos Franco, Ana Cecília Ribeiro Cruz, Janniffer Oliveira Chiang, Livia Carício Martins, Juliana Abreu Lima Nunes,et al.
MDPI AG
Arboviruses, such as yellow fever virus (YFV), dengue virus (DENV), and chikungunya virus (CHIKV), present wide global dissemination and a pathogenic profile developed in infected individuals, from non-specific clinical conditions to severe forms, characterised by the promotion of significant lesions in different organs of the harbourer, culminating in multiple organ dysfunction. An analytical cross-sectional study was carried out via the histopathological analysis of 70 samples of liver patients, collected between 2000 and 2017, with confirmed laboratory diagnoses, who died due to infection and complications due to yellow fever (YF), dengue fever (DF), and chikungunya fever (CF), to characterise, quantify, and compare the patterns of histopathological alterations in the liver between the samples. Of the histopathological findings in the human liver samples, there was a significant difference between the control and infection groups, with a predominance of alterations in the midzonal area of the three cases analysed. Hepatic involvement in cases of YF showed a greater intensity of histopathological changes. Among the alterations evaluated, cell swelling, microvesicular steatosis, and apoptosis were classified according to the degree of tissue damage from severe to very severe. Pathological abnormalities associated with YFV, DENV, and CHIKV infections showed a predominance of changes in the midzonal area. We also noted that, among the arboviruses studied, liver involvement in cases of YFV infection was more intense.
Jorge Rodrigues de Sousa, Luiz Fábio Magno Falcão, Gabriela Lobato Virgolino, Matheus Ferreira Santos Cruz, Vinicius Fialho Teixeira, Tinara Leila de Souza Aarão, Ismari Perinii Furlaneto, Francisca Regina Oliveira Carneiro, Gabriela Amin, Hellen Thais Fuzii,et al.
Microbial Pathogenesis Elsevier BV
Adenauer Marinho de Oliveira Góes Junior, Karlene Thayane Barros da Silva, Ismari Perini Furlaneto, and Simone de Campos Vieira Abib
Elsevier BV
Luciano A. Favorito
FapUNIFESP (SciELO)
1 Graduate student, School of Medicine, Centro Universitário do Estado do Pará, Belém-PA, Brazil; 2 MSc, Associate Professor, Department of Urology, School of Medicine, Centro Universitário do Estado do Pará, Belém-PA, Brazil; 3 PhD, Grupo de Pesquisa Experimental, Centro Universitário do Estado do Pará, Belém-PA, Brazil; 4 MSc, Associate Professor, Department of Radiology, School of Medicine, Centro Universitário do Estado do Pará, Belém-PA, Brazil
Jasna Letícia Pinto Paz, Maria do Perpétuo Socorro Corrêa Amador Silvestre, Letícia Siqueira Moura, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Karla Valéria Batista Lima, and Luana Nepomuceno Gondim Costa Lima
Portland Press Ltd.
Abstract The transmission and evolution of leprosy depends on several aspects, including immunological and genetic factors of the host, as well as genetic factors of Mycobacterium leprae. The present study evaluated the association of single nucleotide polymorphisms (SNPs) on the FokI (rs2228570), TaqI (rs731236), ApaI (rs7975232) regions of the vitamin D receptor (VDR) gene with leprosy. A total of 405 individuals were evaluated, composed by groups of 100 multibacillary (MB) and 57 paucibacillary (PB) patients, and 248 healthy contacts. Blood samples were collected from patients and contacts. The genotyping was performed by sequencing of the interest regions. The alleles of the studied SNPs, and SNP FokI genotypes, were not associated with leprosy. For the SNP on TaqI region, the relationship between the tt genotype, and for the SNP ApaI, the AA genotype, revealed an association with susceptibility to MB form, while Aa genotype with protection. The extended genotypes AaTT and AaTt of ApaI and TaqI were associated with protection against MB form. Further studies analyzing the expression of the VDR gene and the correlation with its SNPs might help to clarify the role of polymorphisms on the immune response in leprosy.
Silvia Cristina Da Silva Pedroso, Karla Valéria Batista Lima, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Darlene Kássia Saraiva Queiroz Pantoja, Alex Junior Souza de Souza, and Washington Luiz Assunção Pereira
Journal of Infection in Developing Countries
Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region.
 Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively.
 Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.
 Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil.
Blenda Gonçalves Cabral, Danielle Murici Brasiliense, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, and Karla Valéria Batista Lima
MDPI AG
Surgical site infection (SSI) following caesarean section is associated with increased morbidity, mortality, and significant health care costs. This study evaluated the epidemiological, clinical, and microbiological features of Acinetobacter spp. in women with SSIs who have undergone caesarean section at a referral hospital in the Brazilian Amazon region. This study included 69 women with post-caesarean SSI by Acinetobacter spp. admitted to the hospital between January 2012 and May 2015. The 69 Acinetobacter isolates were subjected to molecular species identification, antimicrobial susceptibility testing, detection of carbapenemase-encoding genes, and genotyping. The main complications of post-caesarean SSI by Acinetobacter were inadequate and prolonged antibiotic therapy, sepsis, prolonged hospitalization, and re-suture procedures. A. baumannii, A. nosocomialis and A. colistiniresistens species were identified among the isolates. Carbapenem resistance was associated with OXA-23-producing A. baumannii isolates and IMP-1-producing A. nosocomialis isolate. Patients with multidrug-resistant A. baumannii infection showed worse clinical courses. Dissemination of persistent epidemic clones was observed, and the main clonal complexes (CC) for A. baumannii were CC231 and CC236 (Oxford scheme) and CC1 and CC15 (Pasteur scheme). This is the first report of a long-term Acinetobacter spp. outbreak in women who underwent caesarean section at a Brazilian hospital. This study demonstrates the impact of multidrug resistance on the clinical course of post-caesarean infections.
Adenauer Marinho de Oliveira Góes Junior, Rosa Helena de Figueiredo Chaves, Ismari Perini Furlaneto, Emanuelle de Matos Rodrigues, Flávia Beatriz Araújo de Albuquerque, Jacob Hindrik Antunes Smit, Carolina Pinheiro de Oliveira, and Simone de Campos Vieira Abib
FapUNIFESP (SciELO)
Resumo Contexto Modelos com animais de médio e grande porte permitem que pesquisadores avaliem a eficácia e a segurança de procedimentos cardiovasculares em sistemas que se assemelham à anatomia humana e podem ser usados para simular cenários para fins de treinamento. Embora modelos suínos tenham sido extensivamente utilizados, muitos fatores fisiológicos e anatômicos permanecem desconhecidos ou apenas superficialmente descritos. Objetivos Descrever a anatomia vascular do suíno por tomografia computadorizada, compará-la à anatomia humana e discutir a aplicação dos modelos porcinos em procedimentos abertos e endovasculares. Métodos Três porcos machos da raça Landrace foram submetidos a tomografia computadorizada. A anatomia vascular de pescoço, tórax, abdome e membros foi analisada e descrita; foram destacadas similaridades e divergências relevantes entre a anatomia vascular de suínos e de humanos e as implicações em procedimentos vasculares nos suínos. Resultados O território carotídeo, o arco aórtico e os ramos terminais da aorta em suínos apresentaram diferenças marcantes quando comparados aos de humanos. Foram detectadas compressões de veias renal e ilíaca comum, ambas à esquerda, semelhantes às encontradas nas síndromes humanas de Nutcracker e May-Thurner. Medidas vasculares (diâmetro, comprimento e ângulos) de diferentes topografias de suínos foram fornecidas. Conclusões Os dados fornecidos podem ser úteis para o planejamento de ensaios pré-clínicos e pesquisa básica, bem como para o refinamento do treinamento cirúrgico usando modelos suínos no campo da cirurgia vascular.
Emilyn Costa Conceição, Richard Steiner Salvato, Karen Machado Gomes, Arthur Emil dos Santos Guimarães, Marília Lima da Conceição, Ricardo José de Paula Souza e Guimarães, Abhinav Sharma, Ismari Perini Furlaneto, Regina Bones Barcellos, Valdes Roberto Bollela,et al.
FapUNIFESP (SciELO)
Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.
Marília Lima da Conceição, Emilyn Costa Conceição, Ismari Perini Furlaneto, Sandro Patroca da Silva, Arthur Emil dos Santos Guimarães, Pedro Gomes, María Laura Boschiroli, Lorraine Michelet, Thomas Andreas Kohl, Katharina Kranzer,et al.
Springer Science and Business Media LLC
AbstractLack of routine surveillance in countries endemic for bovine tuberculosis (TB) and limited laboratory support contributes to the inability to differentiate theMycobacterium tuberculosisComplex species, leading to an underestimated burden of the disease. Here, Whole-Genome Sequencing ofMycobacterium bovisisolated from tissues with TB-like lesions obtained from cattle and buffalos at Marajó Island, Brazil, demonstrates that recent transmission ofM. bovisis ongoing at distinct sites. Moreover, theM. bovisepidemiology in this setting is herein found to be dominated by an endemic and unique clade composed of strains evolved from a common ancestor that are now genetically differentiated from otherM. bovisclades. Additionally, envisioning a rapid strain differentiation and tracing across multiple settings, 28 globally validated strain-specific SNPs were identified, three of which considered as robust markers for theM. bovisMarajó strain. In conclusion, this study contributes with data regarding the identification of a novelM. bovisphylogenetic clade responsible for ongoing transmission events in both cattle and buffalo species in Brazil, provides a framework to investigate the dissemination of this highly prevalent strain and, holds the potential to inform TB control strategies that may help to prevent the spread of bovine and zoonotic TB.
Luana Nepomuceno Gondim Costa Lima, Jasna Letícia Pinto Paz, Maria do Perpétuo Socorro Corrêa Amador Silvestre, Letícia Siqueira Moura, Ismari Perini Furlaneto, and Karla Valéria Batista Lima
MDPI AG
In 2018, 208,619 new cases of leprosy were reported to the World Health Organization (WHO). Of these, 30,957 occurred in the Americas region and 28,660 (92.6% of the total in the Americas) were reported in Brazil. This study aimed to show the reality of the profile of a population in an endemic leprosy area in northern Brazil in relation to age, gender, and bacillus Calmette–Guérin (BCG) vaccination status through the collection of data in the field with the evaluation of the study individuals, who were recruited by spontaneous demand. A total of 405 individuals participated in the study, with 100 multibacillary, 57 paucibacillary, and 248 healthy contacts. A relationship was observed between the occurrence of the disease, as well as the multibacillary form with the largest age group. The male gender was associated with leprosy per se, with the multibacillary form and was the largest representative of the group that was not vaccinated once. BCG vaccination was effective both in protecting against leprosy per se and in the multibacillary form. These results are limited by sample size, may not be conclusive, and will need further confirmation in a larger cohort.
Yan Corrêa Rodrigues, Ismari Perini Furlaneto, Arthur Henrique Pinto Maciel, Ana Judith Pires Garcia Quaresma, Eliseth Costa Oliveira de Matos, Marília Lima Conceição, Marcelo Cleyton da Silva Vieira, Giulia Leão da Cunha Brabo, Edilene do Socorro Nascimento Falcão Sarges, Luana Nepomuceno Godim Costa Lima,et al.
Public Library of Science (PLoS)
Pseudomonas aeruginosa is an opportunistic pathogen causing different types of infections, particularly in intensive care unit patients. Characteristics that favor its persistence artificial environments are related to its high adaptability, wide arsenal of virulence factors and resistance to several antimicrobial classes. Among the several virulence determinants, T3SS stands as the most important due to the clinical impact of exoS and exoU genes in patient’s outcome. The molecular characterization of P. aeruginosa isolates helps in the comprehension of transmission dynamics and enhance knowledge of virulence and resistance roles in infection process. In the present study, we investigated virulence and resistance properties and the genetic background of P. aeruginosa isolated from ICUs patients at a referral hospital in Brazilian Amazon. A total of 54 P. aeruginosa isolates were characterized by detecting 19 virulence-related genes, antimicrobial susceptibility testing, molecular detection of β-lactamase-encoding genes and genotyping by MLST and rep-PCR. Our findings showed high prevalence of virulence-related markers, where 53.7% of the isolates presented at least 17 genes among the 19 investigated (P = 0.01). The rare exoS+/exoU+ cytotoxic virulotype was detected in 55.6% of isolates. Antimicrobial susceptibility testing revealed percentages of antibiotic resistance above 50% to carbapenems, cephalosporins and fluoroquinolones associated to MDR/XDR isolates. Isolates harboring both blaSPM-1 and blaOXA genes were also detected. Genotyping methods demonstrated a wide genetic diversity of strains spread among the different intensive care units, circulation of international MDR/XDR high-risk clones (ST111, ST235, ST244 and ST277) and emergence of seven novel MLST lineages. Finally, our findings highlight the circulation of strains with high virulence potential and resistance to antimicrobials and may be useful on comprehension of pathogenicity process, treatment guidance and establishment of strategies to control the spread of epidemic P. aeruginosa strains.
Jacob Hindrik Antunes Smit, Eduardo Piotto Leonardi, Rosa Helena de Figueiredo Chaves, Ismari Perini Furlaneto, Cezar Massoud Salame da Silva, Simone de Campos Vieira Abib, and Adenauer Marinho de Oliveira Góes Junior
FapUNIFESP (SciELO)
Abstract Purpose: To describe the anatomy of the swine urinary system using computed tomography and to discuss the role of this animal as an experimental model for urological procedures. Methods: Three male Landrace pigs underwent computed tomography and the anatomy of the urinary system and renal circulation was analyzed and described. Results: In all animals, 2 kidneys, 2 ureters and one bladder were identified. Each kidney presented a single renal artery vascularization, with a mean diameter on the right of 4.45 and 5.31 mm on the left (p < 0.0001) and single renal vein drainage, with a mean diameter on the right of 5.78 and 5.82 mm on the left (p = 0.0336). The average renal length was 9.85 cm on the right and 10.30 cm on the left (p < 0.0001). The average renal volume was 113.70 cm3 on the right and 109.70 cm3 on the left (p < 0.0001). The average length of the ureter was 19.78 cm on the right and 22.08 cm on the left (p < 0.0001). The average bladder volume was 423.70 cm3. Conclusions: The data obtained show similarities with human anatomy, suggesting the viability of the swine model for planning preclinical trials, basic research, refinement in experimental surgery and surgical training for urological procedures.
Arthur Emil dos Santos Guimarães, Abhinav Sharma, Ismari Perini Furlaneto, Liliana Rutaihwa, Jedson Ferreira Cardoso, Marília Lima da Conceição, Lizânia Borges Spinassé, Edson Machado, Maria Luiza Lopes, Rafael Silva Duarte,et al.
FapUNIFESP (SciELO)
BACKGROUND
The evaluation of procedures for drug susceptibility prediction of Mycobacterium tuberculosis based on genomic data against the conventional reference method test based on culture is realistic considering the scenario of growing number of tools proposals based on whole-genome sequences (WGS).
OBJECTIVES
This study aimed to evaluate drug susceptibility testing (DST) outcome based on WGS tools and the phenotypic methods performed on isolates of M. tuberculosis Lineage 1 from the state of Pará, Brazil, generally associated with low levels of drug resistance.
METHODOLOGY
Culture based DST was performed using the Proportion Method in Löwenstein-Jensen medium on 71 isolates that had been submitted to WGS. We analysed the seven main genome sequence-based tools for resistance and lineage prediction applied to M. tuberculosis and for comparison evaluation we have used the Kappa concordance test.
FINDINGS
When comparing the WGS-based tools against the DST, we observed the highest level of agreement using TB-profiler. Among the tools, TB-profiler, KvarQ and Mykrobe were those which identified the largest number of TB-MDR cases. Comparing the four most sensitive tools regarding resistance prediction, agreement was observed for 43 genomes.
MAIN CONCLUSIONS
Drug resistance profiling using next-generation sequencing offers rapid assessment of resistance-associated mutations, therefore facilitating rapid access to effective treatment.
Edilene do Socorro Nascimento Falcão Sarges, Yan Corrêa Rodrigues, Ismari Perini Furlaneto, Marcos Vinicios Hino de Melo, Giulia Leão da Cunha Brabo, Kátia Cilene Machado Lopes, Ana Judith Pires Garcia Quaresma, Luana Lima, and Karla Valéria Batista Lima
Informa UK Limited
Purpose Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerbations. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion Our findings revealed a high prevalence of the atypical exoS+/exoU+ virulotype among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+/exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients.
Adenauer Marinho de Oliveira Góes Junior, Rafaela de Souza Araújo, Ismari Perini Furlaneto, and Waldonio de Brito Vieira
FapUNIFESP (SciELO)
Resumo Contexto As síndromes de nutcracker e May-Thurner são raras e, apesar de muitas vezes subdiagnosticadas, podem causar sintomas limitantes de gravidade variável. Frequentemente são consideradas diagnóstico de exclusão e não há consenso na literatura quanto a prevalência, incidência e critérios diagnósticos. Objetivos Estimar a frequência da compressão das veias ilíaca comum e renal esquerdas em tomografias computadorizadas de abdome e pelve. Métodos Estudo descritivo, quantitativo e transversal. Para veia renal esquerda, foram considerados como critérios de compressão a relação diâmetro hilar/aortomesentérico > 4 e o ângulo aortomesentérico < 39° e, para veia ilíaca comum esquerda, o diâmetro < 4 mm. Resultados Foram analisadas tomografias computadorizadas de 95 pacientes; destes, 61% eram mulheres e 39% eram homens. A compressão da veia renal esquerda foi encontrada em 24,2% da amostra, com idade média de 48,8 anos, ocorrendo em 27,6% das mulheres e 18,9% dos homens (p = 0,3366). A compressão da veia ilíaca comum esquerda foi detectada em 15,7% da amostra, com idade média de 45,9 anos, ocorrendo em 24,10% das mulheres e 2,7% dos homens (p = 0,0024). Em 7,4% dos pacientes, ambas compressões venosas foram detectadas. Conclusões A compressão da veia renal esquerda ocorreu em mulheres e homens com frequência semelhante, enquanto a compressão da veia ilíaca comum esquerda foi mais frequente em mulheres. Ambas as compressões venosas foram mais frequentemente encontradas em pacientes com idade entre 41 e 50 anos.
Ismari Perini Furlaneto, Marília Lima da Conceição, Emilyn Costa Conceição, Maria Luíza Lopes, Yan Corrêa Rodrigues, Beatriz Reis Macelino, Harrison Magdinier Gomes, Philip Noel Suffys, Ricardo José de Paula Souza e Guimarães, Marlei Gomes da Silva,et al.
Elsevier BV
Maria Cleonice A. Justino, Erika A. Campos, Joana D’arc P. Mascarenhas, Luana S. Soares, Sylvia de Fátima S. Guerra, Ismari P. Furlaneto, Manoel Jaime C. Pavão Jr, Tassio S. Maciel, Fredison P. Farias, Orvácio M. Bezerra,et al.
Springer Science and Business Media LLC
Tanise Nazaré Maia Costa, Milena Coelho Fernandes Caldato, and Ismari Perini Furlaneto
FapUNIFESP (SciELO)
Resumo O avanço da medicina aprimorou o tratamento de enfermidades, modificando o padrão de morbimortalidade da população, com aumento de doenças crônicas. Esse fenômeno tornou urgente interpretar adequadamente a terminalidade da vida. Considerando essa necessidade, este estudo, de corte transversal, descritivo e analítico, analisou dados sobre a percepção de 111 estudantes do sexto ano de medicina acerca dos cuidados paliativos no fim da vida. Os dados foram coletados entre agosto e novembro de 2016, por meio de questionário e analisados por testes estatísticos com o programa Iramuteq. 37,3% dos estudantes relataram dificuldade em comunicar a morte do paciente à família; 60% sentem-se despreparados ou com dúvidas sobre como lidar com óbitos em serviço de urgência; 25% desconheciam o termo “eutanásia”, 53% “ortotanásia”, e 56% “distanásia”. Os resultados mostram que ainda há lacunas no conhecimento desses estudantes, explicitando a necessidade de que escolas médicas reforcem práticas pedagógicas sobre a morte.
Rosa Helena de Figueiredo Chaves, Celice Cordeiro de Souza, Ismari Perini Furlaneto, Renan Kleber Costa Teixeira, Carolina Pinheiro de Oliveira, Emanuelle de Matos Rodrigues, Daniel Arthur Santos dos Santos, Renata Cunha Silva, Nelson Elias Abrahão da Penha, and Ana Rita de Lima
FapUNIFESP (SciELO)
PURPOSE
To evaluate the influence tramadol on functional recovery of acute spinal cord injury in rats.
METHODS
Ten rats were divided into two groups (n = 5). All animals were submitted by a laminectomy and spinal cord injury at eighth thoracic vertebra. In control group, the rats didn't receive any analgesic. In tramadol group, the rats received tramadol 4mg/Kg at 12/12h until 5 days by subcutaneous. Animals were following by fourteen days. Was evaluated the Basso, Beattie, Bresnahan scale (locomotor evaluation) and Rat Grimace Scale (pain evaluation) at four periods.
RESULTS
There no difference between the groups in locomotor evaluation in all periods evaluated (p>0.05) and in both groups there was a partial recover of function. The tramadol group show a lower pain levels at the first, third and seventh postoperatively days when comparing to the control group.
CONCLUSION
The tramadol as an analgesic agent don't influence on functional recovery of acute spinal cord injury in rats.
Emilyn Costa Conceição, Arthur Emil dos Santos Guimarães, Maria Luíza Lopes, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Marília Lima da Conceição, Wandyra Araújo Barros, Ninarosa Calzavara Cardoso, Abhinav Sharma, Luana Nepomuceno Gondim Costa Lima,et al.
Elsevier BV
José Tadeu Colares Monteiro, Karla Valéria Batista Lima, Adriana Rodrigues Barretto, Ismari Perini Furlaneto, Glenda Moraes Gonçalves, Ana Roberta Fusco da Costa, Maria Luiza Lopes, and Margareth Pretti Dalcolmo
FapUNIFESP (SciELO)
ABSTRACT Objective: To describe the clinical manifestations of patients with pulmonary infection caused by mycobacteria of the Mycobacterium abscessus complex (MABSC), and to compare these manifestations with those of patients infected with other nontuberculous mycobacteria (NTM). Methods: This was a retrospective cohort study involving 43 patients divided into two groups: the MABSC group, consisting of patients with pulmonary infection caused by MABSC (n = 17); and the NTM group, consisting of patients with pulmonary infection caused by NTM other than MABSC (n = 26). Patients were previously treated with a regimen of rifampin, isoniazid, pyrazinamide, and ethambutol before the diagnosis of NTM was confirmed by two culture-positive sputum samples. The nucleotide sequences of the hsp65, 16S rRNA, and/or rpoB genes were analyzed to identify the mycobacteria. Data were collected on demographic, clinical, and radiological characteristics, as well as on treatment responses and outcomes. Results: Loss of appetite was the only clinical manifestation that was significantly more common in the MABSC group than in the NTM group (p = 0.0306). The chance of having to use a second treatment regimen was almost 12 times higher in the MABSC group than in the NTM group. Treatment success was significantly higher in the NTM group than in the MABSC group (83.2% vs. 17.6%; p < 0.0001). The chance of recurrence was approximately 37 times higher in the MABSC group than in the NTM group. Conclusions: In the study sample, treatment response of pulmonary disease caused by MABSC was less favorable than that of pulmonary disease caused by other NTM.
Andressa Miléo Ferraioli Silva, Wanessa Cardoso Praia, Caroline da Silva Alves Palheta, Rodrigo Paracampo Couteiro, Andrew Moraes Monteiro, Luciana Mota Silva, Ismari Perini Furlaneto, Josie Eiras Bisi dos Santos, and Miguel Saraty de Oliveira
GN1 Sistemas e Publicacoes Ltd.
Kelly Emi Hirai, Jorge Rodrigues de Sousa, Luciana Mota Silva, Leônidas Braga Dias Junior, Ismari Perini Furlaneto, Francisca Regina Oliveira Carneiro, Tinara Leila de Souza Aarão, Mirian Nacagami Sotto, and Juarez Antonio Simões Quaresma
Hindawi Limited
Mycobacterium leprae causes leprosy, a dermatoneurological disease which affects the skin and peripheral nerves. One of several cellular structures affected during M. leprae infection is the endoplasmic reticulum (ER). Infection by microorganisms can result in ER stress and lead to the accumulation of unfolded or poorly folded proteins. To restore homeostasis in the cell, the cell induces a series of signaling cascades known as the unfolded protein response called UPR (unfolded protein response). The present work is aimed at investigating the in situ expression of these markers in cutaneous lesions of clinical forms of leprosy and establish possible correlation expression patterns and types of lesion. A total of 43 samples from leprosy patients were analyzed by immunohistochemistry with monoclonal antibodies against GRP78/BiP, PERK, IRE1α, and ATF6. A statistically significant difference between the indeterminate, tuberculoid, and lepromatous clinical forms was detected, with high expression of GRP78/BiP, PERK, IRE1α, and ATF6 in tuberculoid forms (TT) when compared to lepromatous leprosy (LL) and indeterminate (I) leprosy. These results represent the first evidence of ER stress in samples of skin lesions from leprosy patients. We believe that they will provide better understanding of the complex pathogenesis of the disease and facilitate further characterization of the cascade of molecular events elicited during infection.