Interests
Drug Discovery: To take up a challenging research work in Nanotechnology and Bioactive Natural Products Research and contribute significant outcome in connection with animal and human welfare.
EDUCATION
C.Abdul Hakeem College,(Affiliated to Thiruvalluvar University)C.Abdul Hakeem College,(Affiliated to Thiruvalluvar University)
Doctor of Philosophy ( Zoology, ZoologyDoctor of Philosophy ( Zoology, Zoology
2009 - 2014
Activities and societies: Malaria Research and aim to search a new drugActivities and societies: Malaria Research and aim to search a new drug
Screening of medicinal plant extracts for the detection of novel antimalarial compounds against Plasmodium falciparum and Plasmodium berghei
Thiruvalluvar UniversityThiruvalluvar University
Master of Philosophy (MPhil), ZoologyMaster of Philosophy (MPhil), Zoology
2006 - 2007
Grade: 1st Grade
BIOASSAY – GUIDED ISOLATION AND IDENTIFICATION OF MOSQUITO LARVICIDAL COMPOUND FROM INDIGENOUS PLANT EXTRACTS.
Annamalai UniversityAnnamalai University
Bachelor's Degree, B.Ed., Zoology I & IIBachelor's Degree, B.Ed., Zoology I & II
2005 - 2006
Grade: 1st Grade
RESEARCH, TEACHING, or OTHER INTERESTS
Drug Discovery, Animal Science and Zoology, Molecular Biology, Parasitology
58
Scopus Publications
8603
Scholar Citations
47
Scholar h-index
59
Scholar i10-index
Scopus Publications
Green synthesis of silver and titanium dioxide nanoparticles using Euphorbia prostrata extract shows shift from apoptosis to G0/G1 arrest followed by necrotic cell death in Leishmania donovani Abdul Abduz Zahir, Indira Singh Chauhan, Asokan Bagavan, Chinnaperumal Kamaraj, Gandhi Elango, Jai Shankar, Nidhi Arjaria, Selvaraj Mohana Roopan, Abdul Abdul Rahuman, Neeloo Singh Antimicrobial Agents and Chemotherapy, 2015 The aim of the present study was to synthesize silver (Ag) and titanium dioxide (TiO 2 ) nanoparticles (NPs) using green synthesis from aqueous leaf extract of Euphorbia prostrata as antileishmanial agents and to explore the underlying molecular mechanism of induced cell death. In vitro antileishmanial activity of synthesized NPs was tested against promastigotes of Leishmania donovani by alamarBlue and propidium iodide uptake assays. Antileishmanial activity of synthesized NPs on intracellular amastigotes was assessed by Giemsa staining. The leishmanicidal effect of synthesized Ag NPs was further confirmed by DNA fragmentation assay and by cell cycle progression and transmission electron microscopy (TEM) of the treated parasites. TEM analysis of the synthesized Ag NPs showed a spherical shape with an average size of 12.82 ± 2.50 nm, and in comparison to synthesized TiO 2 NPs, synthesized Ag NPs were found to be most active against Leishmania parasites after 24 h exposure, with 50% inhibitory concentrations (IC 50 ) of 14.94 μg/ml and 3.89 μg/ml in promastigotes and intracellular amastigotes, respectively. A significant increase in G 0 /G 1 phase of the cell cycle with a subsequent decrease in S (synthesis) and G 2 /M phases compared to controls was observed. The growth-inhibitory effect of synthesized Ag NPs was attributed to increased length of S phase. A decreased reactive oxygen species level was also observed, which could be responsible for the caspase-independent shift from apoptosis (G 0 /G 1 arrest) to massive necrosis. High-molecular-weight DNA fragmentation as a positive consequence of necrotic cell death was also visualized. We also report that the unique trypanothione/trypanothione reductase (TR) system of Leishmania cells was significantly inhibited by synthesized Ag NPs. The green-synthesized Ag NPs may provide promising leads for the development of cost-effective and safer alternative treatment against visceral leishmaniasis.
Evaluation of antiplasmodial activity of medicinal plants from North Indian Buchpora and South Indian Eastern Ghats Naveen K Kaushik, Asokan Bagavan, Abdul A Rahuman, Abdul A Zahir, Chinnaperumal Kamaraj, Gandhi Elango, Chidambaram Jayaseelan, Arivarasan V Kirthi, Thirunavukkarasu Santhoshkumar, Sampath Marimuthu, Govindasamy Rajakumar, Santosh K Tiwari, Dinkar Sahal Malaria Journal, 2015 BACKGROUND: Development of resistance against the frontline anti-malarial drugs has created an alarming situation, which requires intensive drug discovery to develop new, more effective, affordable and accessible anti-malarial agents. METHODS: Inspired by their ethnobotanical reputation for being effective against febrile diseases, antiplasmodial potential of ethyl acetate extracts (EAE) and methanol extracts (ME) of 17 medicinal plants collected from the Eastern Ghats of South India and Buchpora, North India were explored against Plasmodium falciparum in vitro using the SYBR Green assay. The results were validated both by confirmation that the fall in fluorescence signal was not due to quenching effects mediated by phytochemical extracts and by Giemsa-stained microscopy. RESULTS: Using EAE or ME, promising antiplasmodial activity (IC₅₀ Pf3D7 ≤ 20 μg/ml), was seen in Aerva lanata (Whole aerial parts-EAE), Anisomeles malabarica (Leaf-EAE), Anogeissus latifolia (bark-EAE), Cassia alata (leaves-EAE), Glycyrrhiza glabra (root-EAE), Juglans regia (seed-ME), Psidium guajava (leaf-ME and EAE) and Solanum xanthocarpum (Whole aerial parts-EAE). EAEs from leaves of Couroupita guianensis, Euphorbia hirta, Pergularia daemia, Tinospora cordifolia and Tridax procumbens as also ME from Ricinus communis (leaf and seed) showed good antiplasmodial activity (Pf 3D7 IC₅₀ 21 - 40 μg/ml). Moderate activity (Pf 3D7 IC₅₀: 40-60 μg/mL) was shown by the leaf EAEs of Cardiospermum halicacabum, Indigofera tinctoria and Ricinus communis while the remaining extracts showed marginal (Pf 3D7 IC₅₀ 60 to >100 μg/ml) activities. The promising extracts showed good resistance indices (0.41 - 1.4) against the chloroquine resistant INDO strain of P. falciparum and good selectivity indices (3 to > 22.2) when tested against the HeLa cell line. CONCLUSION: These results provide validity to the traditional medicinal usage of some of these plants and further make a case for activity-guided purification of new pharmacophores against malaria.
Bioassay-guided isolation and characterization of active antiplasmodial compounds from Murraya koenigii extracts against Plasmodium falciparum and Plasmodium berghei Chinnaperumal Kamaraj, Abdul Abdul Rahuman, Selvaraj Mohana Roopan, Asokan Bagavan, Gandhi Elango, Abdul Abduz Zahir, Govindasamy Rajakumar, Chidambaram Jayaseelan, Thirunavukkarasu Santhoshkumar, Sampath Marimuthu, Arivarasan Vishnu Kirthi Parasitology Research, 2014 Malaria is an overwhelming impact in the poorest countries in the world due to their prevalence, virulence and drug resistance ability. Currently, there is inadequate armoury of drugs for the treatment of malaria. This underscores the continuing need for the discovery and development of new effective and safe antimalarial drugs. To evaluate the in vitro and in vivo antimalarial activity of the leaf ethyl acetate extract of Murraya koenigii, bioassay-guided chromatographic fractionation was employed for the isolation and purification of antimalarial compounds. The in vitro antimalarial activity was assayed by the erythrocytic stages of chloroquine-sensitive strain of Plasmodium falciparum (3D7) in culture using the fluorescence-based SYBR Green I assay. The in vivo assay was done by administering mice infected with Plasmodium berghei (NK65) four consecutive daily doses of the extracts through oral route following Peter's 4-day curative standard test. The percentage suppression of parasitaemia was calculated for each dose level by comparing the parasitaemia in untreated control with those of treated mice. Cytotoxicity was determined against HeLa cells using MTT assay. Histopathology was studied in kidney, liver and spleen of isolated compound-treated Swiss albino mice. The leaf crude ethyl acetate extract of M. koenigii showed good in vitro antiplasmodial activity against P. falciparum. The in vivo test of the leaf crude ethyl acetate extract (600 mg/kg) showed reduced malaria parasitaemia by 86.6% against P. berghei in mice. Bioassay-guided fractionation of the leaf ethyl acetate extract of M. koenigii led to the isolation of two purified fractions C3B2 (2.84 g) and C3B4 (1.97 g). The purified fractions C3B2 and C3B4 were found to be active with IC50 values of 10.5 ± 0.8 and 8.25 ± 0.2 μg/mL against P. falciparum, and in vivo activity significantly reduced parasitaemia by 82.6 and 88.2% at 100 mg/kg/body weight on day 4 against P. berghei, respectively. The isolated fractions C3B2 and C3B4 were monitored by thin-layer chromatography until a single spot was obtained with R f values of 0.36 and 0.52, respectively. The pure compounds obtained in the present investigation were subjected to UV-visible spectroscopy, Fourier transformer infrared spectroscopy, 1D and 2D (1)H-Nuclear magnetic resonance (NMR), (13)C NMR, DEPT, COSY and Mass spectral analysis. Based on the spectral analysis, it is concluded that the isolated compounds were myristic acid (C3B2) and β-caryophyllene (C3B4). The cytotoxic effect of myristic acid and β-caryophyllene showed the TC50 values of >100 and 80.5 μg/mL, respectively against HeLa cell line. The histopathology study showed that protection against nephrotoxicity of kidney, hepatic damage of liver and splenocytes protection in spleen was achieved with the highest dose tested at 100 mg/kg/body weight. The present study provides evidence of antiplasmodial compounds from M. koenigii and is reported for the first time.
Acaricidal activity of synthesized titanium dioxide nanoparticles using Calotropis gigantea against Rhipicephalus microplus and Haemaphysalis bispinosa Sampath Marimuthu, Abdul Abdul Rahuman, Chidambaram Jayaseelan, Arivarasan Vishnu Kirthi, Thirunavukkarasu Santhoshkumar, Kanayairam Velayutham, Asokan Bagavan, Chinnaperumal Kamaraj, Gandhi Elango, Moorthy Iyappan, Chinnadurai Siva, Loganathan Karthik, Kokati Venkata Bhaskara Rao Asian Pacific Journal of Tropical Medicine, 2013 OBJECTIVE: To assess the acaricidal activity of titanium dioxide nanoparticles (TiO2 NPs) synthesized from flower aqueous extract of Calotropis gigantea(C. gigantea) against the larvae of Rhipicephalus (Boophilus) microplus [R. (B.) microplus] and the adult of Haemaphysalis bispinosa (H. bispinosa). METHODS: The lyophilized C. gigantea flower aqueous extract of 50 mg was added with 100 mL of TiO(OH)2 (10 mM) and magnetically stirred for 6 h. Synthesized TiO2 NPs were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and Energy dispersive X-ray spectroscopy (EDX). The synthesised TiO2 NPs were tested against the larvae of R. (B.) microplus and adult of H. bispinosa were exposed to filter paper impregnated method. RESULTS: XRD confirmed the crystalline nature of the nanoparticles with the mean size of 10.52 nm. The functional groups for synthesized TiO2 NPs were 1 405.19, and 1 053.45 cm(-1) for -NH2 bending, primary amines and amides and 1 053.84 and 1 078.45 cm(-1) for C-O. SEM micrographs of the synthesized TiO2 NPs showed the aggregated and spherical in shape. The maximum efficacy was observed in the aqueous flower extract of C. gigantea and synthesized TiO2 NPs against R. (B.) microplus (LC50=24.63 and 5.43 mg/L and r(2)=0.960 and 0.988) and against H. bispinosa (LC50= 35.22 and 9.15 mg/L and r(2) = 0.969 and 0.969), respectively. CONCLUSIONS: The synthesized TiO2 NPs were highly stable and had significant acaricidal activity against the larvae of R. (B.) microplus and adult of H. bispinosa. This study provides the first report of synthesized TiO2 NPs and possessed excellent anti-parasitic activity.
Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G 0 /G 1 Arrest followed by Necrotic … AA Zahir, IS Chauhan, A Bagavan, C Kamaraj, G Elango, J Shankar, ... Antimicrobial agents and chemotherapy 59 (8), 4782-4799 , 2015 2015 Citations: 209
Synthesis and larvicidal activity of low-temperature stable silver nanoparticles from psychrotolerant Pseudomonas mandelii A Mageswari, P Subramanian, V Ravindran, S Yesodharan, A Bagavan, ... Environmental Science and Pollution Research 22 (7), 5383-5394 , 2015 2015 Citations: 39
Evaluation of antiplasmodial activity of medicinal plants from North Indian Buchpora and South Indian Eastern Ghats NK Kaushik, A Bagavan, AA Rahuman, AA Zahir, C Kamaraj, G Elango, ... Malaria journal 14 (1), 65 , 2015 2015 Citations: 122
Synthesis of nanoparticles using Euphorbia prostrata extract reveals a shift from apoptosis to G0/G1 arrest in Leishmania donovani AA Zahir, IS Chauhan, A Bagavan, C Kamaraj, G Elango, J Shankar, ... J. Nanomed. Nanotechnol 5 (4) , 2014 2014 Citations: 29
Bioassay-guided isolation and characterization of active antiplasmodial compounds from Murraya koenigii extracts against Plasmodium falciparum and Plasmodium berghei C Kamaraj, AA Rahuman, SM Roopan, A Bagavan, G Elango, AA Zahir, ... Parasitology research 113 (5), 1657-1672 , 2014 2014 Citations: 38
Solanum trilobatum extract-mediated synthesis of titanium dioxide nanoparticles to control Pediculus humanus capitis, Hyalomma anatolicum anatolicum and Anopheles subpictus G Rajakumar, AA Rahuman, C Jayaseelan, T Santhoshkumar, ... Parasitology Research 113 (2), 469-479 , 2014 2014 Citations: 151
Synthesis of Nanoparticles Using Euphorbia prostrata Extract Reveals a Shift from Apoptosis to G0/G1 Arrest in Leishmania donovani. J Nanomed Nanotechnol 5: 213. doi: 10.4172 … AA Zahir, IS Chauhan, A Bagavan, C Kamaraj, G Elango Euphorbia prostrata , 2014 2014
Acaricidal activity of synthesized titanium dioxide nanoparticles using Calotropis gigantea against Rhipicephalus microplus and Haemaphysalis bispinosa S Marimuthu, AA Rahuman, C Jayaseelan, AV Kirthi, T Santhoshkumar, ... Asian Pacific journal of tropical medicine 6 (9), 682-688 , 2013 2013 Citations: 204
Antiplasmodial potential of selected medicinal plants from eastern Ghats of South India NK Kaushik, A Bagavan, AA Rahuman, D Mohanakrishnan, C Kamaraj, ... Experimental Parasitology 134 (1), 26-32 , 2013 2013 Citations: 71
Novel and simple approach using synthesized nickel nanoparticles to control blood-sucking parasites G Rajakumar, AA Rahuman, K Velayutham, J Ramyadevi, ... Veterinary Parasitology 191 (3-4), 332-339 , 2013 2013 Citations: 53
Feeding deterrent activity of synthesized silver nanoparticles using Manilkara zapota leaf extract against the house fly, Musca domestica (Diptera: Muscidae) C Kamaraj, G Rajakumar, AA Rahuman, K Velayutham, A Bagavan, ... Parasitology research 111 (6), 2439-2448 , 2012 2012 Citations: 99
Evaluation of Catharanthus roseus leaf extract-mediated biosynthesis of titanium dioxide nanoparticles against Hippobosca maculata and Bovicola ovis K Velayutham, AA Rahuman, G Rajakumar, T Santhoshkumar, ... Parasitology research 111 (6), 2329-2337 , 2012 2012 Citations: 242
Lousicidal activity of synthesized silver nanoparticles using Lawsonia inermis leaf aqueous extract against Pediculus humanus capitis and Bovicola ovis S Marimuthu, AA Rahuman, T Santhoshkumar, C Jayaseelan, AV Kirthi, ... Parasitology research 111 (5), 2023-2033 , 2012 2012 Citations: 84
Efficacy of adulticidal and larvicidal properties of botanical extracts against Haemaphysalis bispinosa , Hippobosca maculata , and Anopheles subpictus T Santhoshkumar, AA Rahuman, A Bagavan, AV Kirthi, S Marimuthu, ... Parasitology research 111 (4), 1833-1840 , 2012 2012 Citations: 11
Evaluation of antileishmanial activity of South Indian medicinal plants against Leishmania donovani AA Zahir, AA Rahuman, S Pakrashi, D Ghosh, A Bagavan, C Kamaraj, ... Experimental parasitology 132 (2), 180-184 , 2012 2012 Citations: 42
Evaluation of stem aqueous extract and synthesized silver nanoparticles using Cissus quadrangularis against Hippobosca maculata and Rhipicephalus (Boophilus) microplus T Santhoshkumar, AA Rahuman, A Bagavan, S Marimuthu, C Jayaseelan, ... Experimental Parasitology 132 (2), 156-165 , 2012 2012 Citations: 120
Evaluation of medicinal plant extracts and isolated compound epicatechin from Ricinus communis against Paramphistomum cervi AA Zahir, AA Rahuman, A Bagavan, K Geetha, C Kamaraj, G Elango Parasitology research 111 (4), 1629-1635 , 2012 2012 Citations: 47
Antiplasmodial potential of medicinal plant extracts from Malaiyur and Javadhu hills of South India C Kamaraj, NK Kaushik, D Mohanakrishnan, G Elango, A Bagavan, ... Parasitology Research 111 (2), 703-715 , 2012 2012 Citations: 79
Efficacy of plant-mediated synthesized silver nanoparticles against hematophagous parasites C Jayaseelan, AA Rahuman, G Rajakumar, T Santhoshkumar, AV Kirthi, ... Parasitology research 111 (2), 921-933 , 2012 2012 Citations: 99
Antimalarial activities of medicinal plants traditionally used in the villages of Dharmapuri regions of South India C Kamaraj, NK Kaushik, AA Rahuman, D Mohanakrishnan, A Bagavan, ... Journal of Ethnopharmacology 141 (3), 796-802 , 2012 2012 Citations: 115
MOST CITED SCHOLAR PUBLICATIONS
Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi C Jayaseelan, AA Rahuman, AV Kirthi, S Marimuthu, T Santhoshkumar, ... Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 90, 78-84 , 2012 2012 Citations: 1052
Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors T Santhoshkumar, AA Rahuman, G Rajakumar, S Marimuthu, A Bagavan, ... Parasitology research 108 (3), 693-702 , 2011 2011 Citations: 610
Evaluation of green synthesized silver nanoparticles against parasites S Marimuthu, AA Rahuman, G Rajakumar, T Santhoshkumar, AV Kirthi, ... Parasitology research 108 (6), 1541-1549 , 2011 2011 Citations: 441
Synthesis of pediculocidal and larvicidal silver nanoparticles by leaf extract from heartleaf moonseed plant, Tinospora cordifolia Miers C Jayaseelan, AA Rahuman, G Rajakumar, A Vishnu Kirthi, ... Parasitology research 109 (1), 185-194 , 2011 2011 Citations: 326
Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae) C Kamaraj, A Bagavan, AA Rahuman, AA Zahir, G Elango, G Pandiyan Parasitology research 104 (5), 1163-1171 , 2009 2009 Citations: 276
Larvicidal activity of saponin from Achyranthes aspera against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) A Bagavan, AA Rahuman, C Kamaraj, K Geetha Parasitology research 103 (1), 223-229 , 2008 2008 Citations: 263
Evaluation of Catharanthus roseus leaf extract-mediated biosynthesis of titanium dioxide nanoparticles against Hippobosca maculata and Bovicola ovis K Velayutham, AA Rahuman, G Rajakumar, T Santhoshkumar, ... Parasitology research 111 (6), 2329-2337 , 2012 2012 Citations: 242
Biosynthesis of titanium dioxide nanoparticles using bacterium Bacillus subtilis AV Kirthi, AA Rahuman, G Rajakumar, S Marimuthu, T Santhoshkumar, ... Materials Letters 65 (17-18), 2745-2747 , 2011 2011 Citations: 232
Efficacy of plant-mediated synthesized silver nanoparticles against Sitophilus oryzae AA Zahir, A Bagavan, C Kamaraj, G Elango, AA Rahuman Journal of Biopesticides 5, 95 , 2012 2012 Citations: 221
Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say (Diptera: Culicidae) AA Rahuman, A Bagavan, C Kamaraj, E Saravanan, AA Zahir, G Elango Parasitology Research 104 (6), 1365-1372 , 2009 2009 Citations: 211
Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G 0 /G 1 Arrest followed by Necrotic … AA Zahir, IS Chauhan, A Bagavan, C Kamaraj, G Elango, J Shankar, ... Antimicrobial agents and chemotherapy 59 (8), 4782-4799 , 2015 2015 Citations: 209
Larvicidal activity of medicinal plant extracts against Anopheles subpictus & Culex tritaeniorhynchus C Kamaraj, A Bagavan, G Elango, AA Zahir, G Rajakumar, S Marimuthu, ... Indian Journal of Medical Research 134 (1), 101-106 , 2011 2011 Citations: 209
Acaricidal activity of synthesized titanium dioxide nanoparticles using Calotropis gigantea against Rhipicephalus microplus and Haemaphysalis bispinosa S Marimuthu, AA Rahuman, C Jayaseelan, AV Kirthi, T Santhoshkumar, ... Asian Pacific journal of tropical medicine 6 (9), 682-688 , 2013 2013 Citations: 204
Antifeedant and larvicidal effects of plant extracts against Spodoptera litura (F.), Aedes aegypti L. and Culex quinquefasciatus Say C Kamaraj, AA Rahuman, A Bagavan Parasitology research 103 (2), 325-331 , 2008 2008 Citations: 204
In vitro antimalarial activity of medicinal plant extracts against Plasmodium falciparum A Bagavan, AA Rahuman, NK Kaushik, D Sahal Parasitology research 108 (1), 15-22 , 2011 2011 Citations: 198
Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae) G Elango, A Bagavan, C Kamaraj, A Abduz Zahir, A Abdul Rahuman Parasitology research 105 (6), 1567-1576 , 2009 2009 Citations: 170
Laboratory determination of efficacy of indigenous plant extracts for parasites control AA Zahir, AA Rahuman, C Kamaraj, A Bagavan, G Elango, A Sangaran, ... Parasitology Research 105 (2), 453-461 , 2009 2009 Citations: 167
Solanum trilobatum extract-mediated synthesis of titanium dioxide nanoparticles to control Pediculus humanus capitis, Hyalomma anatolicum anatolicum and Anopheles subpictus G Rajakumar, AA Rahuman, C Jayaseelan, T Santhoshkumar, ... Parasitology Research 113 (2), 469-479 , 2014 2014 Citations: 151
Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus G Elango, AA Rahuman, A Bagavan, C Kamaraj, AA Zahir, C Venkatesan Parasitology Research 104 (6), 1381-1388 , 2009 2009 Citations: 149
Evaluation of indigenous plant extracts against larvae of Culex quinquefasciatus Say (Diptera: Culicidae) AA Rahuman, A Bagavan, C Kamaraj, M Vadivelu, AA Zahir, G Elango, ... Parasitology Research 104 (3), 637-643 , 2009 2009 Citations: 146