Davide Guido
@unipv.it
University of Pavia
Scopus Publications
- Efficacy and safety of mesh reinforced cruroplasty with Phasix™ ST vs. Bio-A®: systematic review and bayesian meta-analysis
Michele Manara, Davide Bona, Sara De Bernardi, Marta Cavalli, Quan Wang, et al.
Hernia, 2026 - Proteoglycan-4 potentiates the antitumor efficacy of regorafenib in an orthotopic model of hepatocellular carcinoma
Livianna Carrieri, Anas Munir, Giusi Caragnano, Davide Guido, Grazia Serino, et al.
Journal of Experimental and Clinical Cancer Research, 2026
Background Hepatocellular carcinoma (HCC) presents a significant therapeutic challenge, as current treatment options provide limited long-term benefits due to issues surrounding their effectiveness and associated adverse effects. Our previous research demonstrated that Proteoglycan-4 (PRG4) enhances the anti-proliferative effect of the multi-kinase inhibitor regorafenib in simple in vitro two-dimensional HCC models. In this study, we aimed to investigate the potential adjuvant role of PRG4 in improving the efficacy of regorafenib within both three-dimensional in vitro and in vivo HCC models. Methods Human HCC cells were engineered to stably overexpress PRG4. The effects of PRG4 on cell proliferation, both alone and in combination with regorafenib, were tested in monolayer cultures, Matrigel-embedded spheroids, and an orthotopic xenograft HCC mouse model. Additionally, transcriptomic profiling of spheroids generated from control or PRG4-overexpressing HCC cells, either untreated or treated with regorafenib, was performed. Results PRG4 expression partially inhibited HCC tumor growth in vivo and enhanced regorafenib antiproliferative activity, leading to a near-complete tumor regression. This synergistic PRG4 + regorafenib interaction in impairing HCC cell growth was further confirmed in 2D and 3D HCC models in vitro. In addition, PRG4 restrained angiogenesis by hindering endothelial tubulogenesis in vitro. By transcriptomic analysis of matrigel-embedded HCC cell spheroids exposed to PRG4 and/or regorafenib, PDGF pathway emerged as a target of PRG4 + regorafenib, corroborating the role of PRG4 in impairing angiogenesis. The G 0 /G 1 phase of the cell cycle was more delayed in spheroids exposed to both PRG4 and regorafenib compared to those treated with regorafenib alone, relative to untreated cells. Conclusions PRG4 demonstrated antitumor activities in vivo and shows promise as an adjuvant to enhance therapeutic interventions in HCC. - Effect of 4-Week Consumption of “Navelina” Oranges on Serum Lipid Profile in Patients with MASLD: Evidence from a Randomized Clinical Trial
Valentina De Nunzio, Giuliano Pinto, Davide Guido, Emanuela Aloisio Caruso, Miriam Cofano, et al.
Nutrients, 2026
Background: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) refers to fatty liver disease associated with metabolic syndrome. MASLD causes alterations in lipid metabolism, which can be regulated with a diet rich in polyphenols. The present study aims to evaluate the effects of daily consumption of 400 g of “Navelina” oranges for 4 weeks on serum lipid profiles in a group of 60 patients with MASLD, to identify specific lipid species associated with improvements in hepatic steatosis. Methods: Blood samples were collected from all participants, and biochemical measurements and a serum lipidomic profile were performed. Finally, a Spearman correlation analysis was used to assess the relationships between serum lipidomic fatty acids and biochemical lipid markers. Results: In the experimental treatment arm, serum lipidomic analysis showed a slight decrease in Arachidonic acid (AA) and the Arachidonic acid/Eicosapentaenoic acid ratio (AA/EPA ratio) but no significant interaction between time and treatment was detected. In the same group, Oleic acid, MUFAs and the AA/EPA ratio were significantly and negatively correlated with HDL (r = −0.368, p = 0.046), (r = −0.384, p = 0.036), and (r = −0.522, p = 0.003), respectively. Conversely, EPA and n-3 PUFAs were positively and significantly correlated with HDL (r = 0.447, p = 0.013) and (r = 0.403, p = 0.027) respectively. Conclusions: Furthermore, this study represents one of the first clinical trials to shed a light on the potential association of “Navelina” orange polyphenols on serum fatty acid profiles in patients with MASLD, supporting studies on the nutraceutical effect of oranges on lipid metabolism. - A Dose–Response Study on the Relationship Between Red Meat Intake and Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in Southern Italy: Results from the Nutrihep Study
Davide Guido, Manuela Siani, Maria Noemy Pastore, Gianluigi Giannelli, Giovanni De Pergola
Nutrients, 2026
(1) Background: Metabolic dysfunction-associated liver disease (MASLD) has emerged as a leading cause of liver conditions globally. The increasing trend in meat consumption, particularly red meat, has prompted examination of its effects on cardiometabolic health. This study aimed to explore how varying levels of red meat intake relate to MASLD in a population from Southern Italy. (2) Methods: We analyzed data from a cross-sectional study involving 1192 participants (42.7% male), with complete data available from the second NUTRIHEP survey wave (2014–2016). Statistical analysis utilized adjusted dose–response modeling. (3) Results: Subjects with MASLD numbered 587 (49.2%), including 278 males (54.6%) and 309 females (45.2%). Red meat consumption between 75 and 90 g/day revealed an unfavorable influence on MASLD in males. Interestingly, sex seem to play a role in this association, both in harmful (OR > 1) and protective (OR < 1) ways, associated with specific foods such as liver (OR = 0.936, p = 0.087) and red meatballs (OR = 0.584, p = 0.023) in males and roast red meat (OR = 2.152, p = 0.097), red cutlet (OR = 0.540, p = 0.087), and red meat slices (OR = 0.952, p = 0.076) in females. (4) Conclusions: A suspicious dose–response relationship was observed solely in men, limited to intake levels between 75 and 90 g/day. Overall, red meat consumption did not exhibit a consistent dose–response trend with MASLD. Furthermore, preferences for specific types, cuts, and preparations of red meat were differentially associated with metabolic outcomes based on sex. - Economic value and clinical association of a supervised lifestyle-improving program for MASLD
Maurizio Polignano, Antonella Bianco, Davide Guido, Pietro Trisolini, Isabella Franco, et al.
Frontiers in Pharmacology, 2026
Background Metabolic dysfunction-associated steatotic liver disease (MASLD) is both common and, in some cases, a progressive condition. Emerging pharmacological options have shown promise in select patient sub-groups (e.g., resmetirom for MASH with fibrosis; GLP-1 receptor agonists for obesity/diabetes with metabolic benefits), but structured lifestyle programs remain foundational in routine care. Objective This study evaluates the cost–utility analysis of a multidisciplinary, kinesiology-supervised lifestyle-improving program for patients with MASLD, supported by clinical evidence. Methods We analyzed 27 adults with MASLD, a cohort established from an initial group of 43 subjects, who participated in a structured program of supervised exercise and dietary counseling. Health-related quality of life (SF-36 mapped to EQ-5D) and associated clinical markers, including hepatic steatosis (ultrasound), blood pressure, and serum aminotransferases, were evaluated at baseline and after the program. A cost–utility analysis was conducted from the healthcare system’s perspective, estimating the incremental cost-effectiveness ratios (ICERs and €/QALY) with deterministic and probabilistic sensitivity analyses. Pharmaceutical expenditures and projected disease progression costs were also explored using administrative data and literature-based models. Results Health-related quality of life improved after the program, with a quality-adjusted life year (QALY) gain of 0.081 (95% CI: 0.001–0.161). The base-case ICER was €17,778/QALY. The probability of cost-effectiveness was 71% at €25,000/QALY, 84% at €30,000/QALY, and 95% at €40,000/QALY. Ultrasound steatosis showed a distributional shift toward lower grades with an unchanged median (Wilcoxon p = 0.007). Systolic/diastolic blood pressure decreased by −5.6/−3.7 mmHg (p = 0.05 and p = 0.03), and AST/ALT declined (both p &lt; 0.01). At the 2-year follow-up, 55.6% of patients reported maintaining regular physical activity. Outpatient pharmaceutical expenditures showed a decline from €74 to €50 per patient/year between 2018 and 2021, with reduced variability across patients. However, this trend did not reach statistical significance in mixed-effects analyses (p = 0.06). Conclusion In this pre–post observational study, the supervised program was associated with favorable cost–utility outcomes and distributional improvements in selected clinical markers. These findings support the program’s potential value in routine care and warrant confirmation in controlled studies. Clinical Trial Registration https://clinicaltrials.gov/expert-search?term , identifier NCT06026293. - Liver steatosis is positively associated with plasminogen activator inhibitor-1 in apparently healthy individuals with overweight and obesity: A FibroScan-Based Cross-Sectional study
Giuseppe Lisco, Davide Guido, Nicole Cerabino, Martina Di Chito, Rosanna Donvito, et al.
Journal of Translational Medicine, 2025 - Exploring miRNA Research in Colorectal Cancer: Insights from a Bibliometric Analysis
Emanuele Piccinno, Michelangelo Aloisio, Viviana Scalavino, Francesco Russo, Gianluigi Giannelli, et al.
Pharmaceutics, 2025
Background/Objectives: Despite advances in diagnosis and treatment, colorectal cancer (CRC) remains one of the most prevalent and challenging malignancies worldwide. The dysregulation of microRNAs (miRNAs) has emerged as a critical factor in CRC onset, progression, and therapeutic resistance. This study aims to provide an overview of global research trends on miRNAs in CRC, (i) identifying the most studied miRNAs, (ii) exploring under-investigated areas, and (iii) highlighting emerging themes and potential future directions. Methods: To assess the evolution of the global miRNA–CRC research trends, we conducted a bibliometric analysis of 828 CRC–miRNA-focused articles published between 2008 and 2024, sourced from the Scopus database. Bibliometric mapping was performed using the R/Bibliometrix package and by leveraging a customized Python-based pipeline, which is useful for extracting and validating miRNA identifiers (miRNA IDs) based on the miRBase database. This miRNA ID-related approach enabled us to systematically identify the most frequently studied miRNAs over time while highlighting underexplored miRNA. Results: The analysis revealed a substantial and accelerating publication growth rate, delineating three major phases in CRC–miRNA research. China emerged as the leading contributor in terms of the publication volume. miR-21, miR-34a, and miR-195-5p were among the most frequently studied miRNAs, underscoring their relevance to CRC biology and therapy. Keyword and citation analyses identified key thematic areas, such as cell proliferation, epithelial–mesenchymal transition, and chemoresistance, especially to oxaliplatin and 5-fluorouracil. Emerging research frontiers included ferroptosis, ceRNA networks, and exosome-mediated miRNA transport. An analysis of the collaborations indicated strong intra-national collaborations, with room for expanding international research networks. Conclusions: This study provides an in-depth bibliometric landscape of the CRC-related miRNA research by highlighting influential studies and journals while identifying gaps and underexplored topics. These insights offer valuable guidance for future translational and clinical research on this topic. - Ion Channel–Extracellular Matrix Interplay in Colorectal Cancer: A Network-Based Approach to Tumor Microenvironment Remodeling
Alberta Terzi, Fatima Maqoud, Davide Guido, Domenica Mallardi, Michelangelo Aloisio, et al.
International Journal of Molecular Sciences, 2025
The progression of colorectal cancer (CRC) is driven by dynamic interactions between tumor cells and their microenvironment, particularly the extracellular matrix (ECM). Ion channels, critical regulators of cellular signaling, have emerged as mediators of ECM remodeling and tumor aggressiveness. In this study, we integrate transcriptomic data from 185 CRC tumors and 157 adjacent normal tissues with network modeling to dissect the interplay between ion channels and the ECM. We identified 4036 differentially expressed genes (DEGs), including 188 ion channel-associated DEGs (IC-DEGs) enriched in ECM-related pathways, such as collagen assembly, matrix metalloproteinase regulation, and mechanotransduction. Structural equation modeling revealed an active CRC−ion channel module (CRC-IC) comprising 482 nodes and 422 edges, highlighting dysregulated interactions between ECM components (e.g., COL1A1, COL5A2, VCAN, LAMA4, LA-MA5, LAMC1), ion channels (e.g., TRPM5 and SLC16A1), and cytoskeletal regulators. Key nodes, including CHST11 and VCAN, were associated with ECM sulfation, tumor invasiveness, and immune evasion. Notably, survival was associated with MAPK1, SLC16A1, and ABCB4 in relation to patient prognosis. Our findings underscore the pivotal role of ion channels as co-factors in ECM dynamics in CRC, offering mechanistic insights into tumor-stroma crosstalk and identifying potential therapeutic targets to disrupt microenvironment-driven progression. - Liver Fibrosis Is Positively and Independently Associated with Leptin Circulating Levels in Individuals That Are Overweight and Obese: A FibroScan-Based Cross-Sectional Study
Nicole Cerabino, Martina Di Chito, Davide Guido, Vincenza Di Stasi, Caterina Bonfiglio, et al.
Nutrients, 2025
Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is strongly correlated with the severity of obesity, and the extent of liver fibrosis is associated with a higher risk of liver-related complications, cardiovascular events, and overall mortality. Leptin circulating levels are directly correlated with the amount of adipose tissue. Aims: In the present study, we investigated the association between circulating leptin levels and liver steatosis and fibrosis. Methods: Eighty-six patients (41.7 ± 12.6 yrs, 35 men, 41%), naïve to medications, who attended the Nutrition Center for the Research and Care of Obesity and Metabolic Diseases at the National Institute of Gastroenterology “Saverio de Bellis” for weight management, were cross-sectionally evaluated. Demographic, anthropometric, clinical, and laboratory data were collected and analyzed. All patients underwent liver ultrasonographic assessment by FibroScan to diagnose liver steatosis (controlled attenuation parameter, CAP > 275 dBm) and fibrosis (liver stiffness measurement, LSM > 8.2 kPa). Results: Sixty-three individuals (73.3%) had liver steatosis, and 17 (19.8%) had liver fibrosis. The mean leptin levels were 22.3 ± 14.1 ng/mL, while the BMI and waist circumference were 36.7 ± 7.2 kg/m2 and 114.5 ± 16.4 cm, respectively. CAP values exhibited no correlation with leptin (r = 0.09, p = 0.436), while a significant connection was seen between leptin and LSM (β = 0.065; p = 0.038). Specifically, for each unit increase in leptin, LSM values were varied by +0.065 units (p = 0.038). This association was independent of gender, age, insulin resistance, adiponectin, RBP4, and visfatin. This is the first study showing these results by using FibroScan assessment in patients naïve to medications. Conclusions: Circulating leptin concentrations are independently correlated with hepatic fibrosis in individuals with a BMI ≥ 25 kg/m2. These findings indicate a function for leptin in promoting liver fibrosis; however, longitudinal studies are required to elucidate the causal nature of this interaction. - Mapping Research Trends on Intestinal Permeability in Irritable Bowel Syndrome with a Focus on Nutrition: A Bibliometric Analysis
Domenica Mallardi, Fatima Maqoud, Davide Guido, Michelangelo Aloisio, Michele Linsalata, et al.
Nutrients, 2025
Irritable Bowel Syndrome (IBS) is a complex gastrointestinal disorder characterized by chronic abdominal pain and altered bowel habits, often linked to disruptions in intestinal barrier function. Increased intestinal permeability plays a key role in IBS pathogenesis, affecting immune responses, gut microbiota, and inflammation. This study conducts a bibliometric analysis to explore global research trends on intestinal permeability in IBS, focusing on key contributors, collaboration networks, and thematic shifts, particularly the interplay between the intestinal barrier, gut microbiota, and dietary components. A total of 411 articles were retrieved from Scopus, with 232 studies analyzed using Bibliometrix in R. To optimize screening, ASReview, a machine learning tool, was employed, utilizing the Naïve Bayes algorithm combined with Term Frequency-Inverse Document Frequency (TF-IDF) for adaptive ranking of articles by relevance. This approach significantly improved screening step efficacy. The analysis highlights growing research interest, with China and the USA as leading contributors. Key themes include the role of gut microbiota in modulating permeability, the impact of dietary components (fiber, probiotics, bioactive compounds) on tight junction integrity, and the exploration of therapeutic agents. Emerging studies suggest integrating gut barrier modulation with nutritional and microbiome-targeted strategies for IBS management. This study provides a comprehensive overview of research on intestinal permeability in IBS, mapping its evolution and identifying major trends. By highlighting key contributors and thematic areas, it offers insights to guide future investigations into the interplay between gut permeability, diet, and microbiota, advancing understanding of IBS pathophysiology and management. - Gastrentrological clinical trials in Italy: an association study on regional economic factors and healthcare system efficiency
Maurizio Polignano, Davide Guido, Giuseppe Dalfino, Gianluigi Giannelli
Frontiers in Pharmacology, 2025 - Association between liver steatosis, fibrosis, and the onset of type 2 diabetes in overweight individuals: A fibroscan-based study in Southern Italy
Davide Guido, Nicole Cerabino, Martina Di Chito, Vincenza Di Stasi, Sara De Nucci, et al.
Diabetes Research and Clinical Practice, 2024 - Exploratory Role of Flavonoids on Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in a South Italian Cohort
Caterina Bonfiglio, Rossella Tatoli, Rossella Donghia, Davide Guido, Gianluigi Giannelli
Antioxidants, 2024 - A Dose–Response Study on the Relationship between White Meat Intake and Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in Southern Italy: Results from the Nutrihep Study
Davide Guido, Nicole Cerabino, Martina Di Chito, Rossella Donghia, Cristiana Randazzo, et al.
Nutrients, 2024 - Transcriptomic Module Discovery of Diarrhea-Predominant Irritable Bowel Syndrome: A Causal Network Inference Approach
Davide Guido, Fatima Maqoud, Michelangelo Aloisio, Domenica Mallardi, Blendi Ura, et al.
International Journal of Molecular Sciences, 2024 - Corrigendum: Time-Trends in Air Pollution Impact on Health in Italy, 1990-2019: An Analysis from the Global Burden of Disease Study 2019
Sara Conti, Carla Fornari, Pietro Ferrara, Ippazio C. Antonazzo, Fabiana Madotto, et al.
International Journal of Public Health, 2024 - Glycemic Index and Amylose Content of 25 Japonica Rice Italian Cultivar
Mariangela Rondanelli, Filip Haxhari, Clara Gasparri, Gaetan Claude Barrile, Alessandro Cavioni, et al.
Starch Staerke, 2023 - The Glycemic Index of Indica and Japonica Subspecies Parboiled Rice Grown in Italy and the Effect on Glycemic Index of Different Parboiling Processes
Mariangela Rondanelli, Riccardo Anselmo Ferrario, Gaetan Claude Barrile, Davide Guido, Clara Gasparri, et al.
Journal of Medicinal Food, 2023 - Discovering the Physio-Pathological Mechanisms of Interaction between Bone Mineral Density, Muscle Mass, and Visceral Adipose Tissue in Female Older Adults through Structural Equation Modeling
Simone Perna, Clara Gasparri, Sabika Allehdan, Antonella Riva, Giovanna Petrangolini, et al.
Journal of Clinical Medicine, 2023 - Endosperm structure and Glycemic Index of Japonica Italian rice varieties
Filip Haxhari, Francesco Savorani, Mariangela Rondanelli, Enrico Cantaluppi, Luigi Campanini, et al.
Frontiers in Plant Science, 2023 - Time-Trends in Air Pollution Impact on Health in Italy, 1990–2019: An Analysis From the Global Burden of Disease Study 2019
Sara Conti, Carla Fornari, Pietro Ferrara, Ippazio C. Antonazzo, Fabiana Madotto, et al.
International Journal of Public Health, 2023 - A Computational Framework for Comprehensive Genomic Profiling in Solid Cancers: The Analytical Performance of a High-Throughput Assay for Small and Copy Number Variants
Luciano Giacò, Fernando Palluzzi, Davide Guido, Camilla Nero, Flavia Giacomini, et al.
Cancers, 2022 - What’s beyond BRCA Mutational Status in High Grade Serous Ovarian Cancer? The Impact of Hormone Receptor Expression in a Large BRCA-Profiled Ovarian Cancer Patient Series: A Retrospective Cohort Study
Emanuele Perrone, Riccardo Tudisco, Pia Clara Pafundi, Davide Guido, Alessandra Ciucci, et al.
Cancers, 2022 - Mapping development and health effects of cooking with solid fuels in low-income and middle-income countries, 2000–18: a geospatial modelling study
Joseph Jon Frostad, QuynhAnh P Nguyen, Mathew M Baumann, Brigette F Blacker, Laurie B Marczak, et al.
Lancet Global Health, 2022 - Bioinformatics: From NGS Data to Biological Complexity in Variant Detection and Oncological Clinical Practice
Serena Dotolo, Riziero Esposito Abate, Cristin Roma, Davide Guido, Alessia Preziosi, et al.
Biomedicines, 2022
