Donald Ramos Perfecto

@unmsm.edu.pe

Docente de la Facultad de Odontologia
Universidad Nacional Mayor de San Marcos



        

https://researchid.co/donaldrp

RESEARCH INTERESTS

Microbiología Bucal
Periodoncia
Productos naturales

6

Scopus Publications

Scopus Publications

  • Increasing Analytical Quality by Designing a Thin-Layer Chromatography Scanner Method for the Determination of the Radiochemical Purity of Radiopharmaceutical Sodium Iodide <sup>131</sup>I Oral Solution
    Miguel Vasquez-Huaman, Américo Castro-Luna, Norma Julia Ramos-Cevallos, Donald Ramos-Perfecto, Mario Alcarraz-Curi, Jacqueline Segura-Vasquez, and Danny Cáceres-Antaurco
    MDPI AG
    The goal of this study was to apply the principles of analytical quality by design (AQbD) to the analytical method for determining the radiochemical purity (PQR) of the radiopharmaceutical sodium iodide 131I oral solution, utilizing thin-layer chromatography (TLC) with a radio–TLC scanner, which also enables the evaluation of product quality. For AQbD, the analytical target profile (ATP), critical quality attributes (CQA), risk management, and the method operable design region (MODR) were defined through response surface methodology to optimize the method using MINITAB® 19 software. This study encompassed the establishment of a control strategy and the validation of the method, including the assessment of selectivity, linearity, precision, robustness, detection limit, quantification limit, range, and the stability of the sample solution. Under the experimental conditions, the method parameters of the TLC scanner were experimentally demonstrated and optimized with an injection volume of 3 µL, a radioactive concentration of 10 mCi/mL, and a carrier volume of 40 µL. Statistical analysis confirmed the method’s selectivity for the 131I iodide band Rf of 0.8, a radiochemical impurity IO3− Rf of 0.6, a linearity from 6.0 to 22.0 mCi/mL, and an intermediate precision with a global relative standard deviation (RSD) of 0.624%. The method also exhibited robustness, with a global RSD of 0.101%, a detection limit of 0.09 mCi/mL, and a quantification limit of 0.53 Ci/mL, meeting the prescribed range and displaying stability over time (at 0, 2, and 20 h) with a global RSD of 0.362%, resulting in consistent outcomes. The development of a method based on AQbD facilitated the creation of a design space and an operational space, with comprehensive knowledge of the method’s characteristics and limitations. Additionally, throughout all operations, compliance with the acceptance criteria was verified. The method’s validity was confirmed under the established conditions, making it suitable for use in the manufacturing process of sodium iodide 131I and application in nuclear medicine services.

  • CAMELLIA SINENSIS, A NATURAL PRODUCT TO SUPPORT THE TREATMENT OF MEDICAL AND STOMATOLOGICAL CONDITIONS
    Ricardo Reyes-Mansilla, , Adelmo Cuentas-Robles, Donald Ramos-Perfecto, , and
    Facultad de Odontologia, Universidad de Concepcion
    Introduction: The tea plant, Camellia sinensis, is one of the most popular non-alcoholic beverages in the world. The main components of Camellia sinensis include amino acids, fatty acids, phenolic compounds, flavins and purine alkaloids (xanthines). For this reason, in the field of medicine, Camellia sinensis has been used as an anticancer, anxiolytic, antidiabetic, antiobesity, anti-inflammatory, analgesic, antipyretic, chemopreventive, cytotoxic and apoptogenic, genoprotective, hepatoprotective, nephroprotective, hemato-protective, and in wound healing, among other uses. Objective: To carry out a systematic review of the use of C. sinensis as supportive therapy in the treatment of oral disorders. Materials and Methods: This systematic review was carried out following the PRISMA guidelines. The search was carried out in the PubMed, ScienceDirect and Google Academic databases. Articles from studies of Camellia sinensis were reviewed and those from a secondary source, such as literature review articles, were excluded Results: A total of 12 full-text articles were selected for review, in which the properties of Camellia sinensis are detailed. Conclusions: According to the bibliography reviewed, C. sinensis exhibits anticariogenic properties, applications in the treatment of dental erosion, applications in the treatment of gingivitis and bacterial plaque, and applications in the prevention of oral cancer; however, more controlled clinical trials are needed to confirm its effectiveness and safety of use. Keywords: Camellia sinensis; Botany; Functional claim; Therapeutics; Dentistry; Systematic Review.

  • Art and science in the diagnosis of atypical periodontal disease: Case report
    LV Maita Véliz, M Castañeda Mosto, LM Maita Castañeda, U Rivas Almonte, and D Ramos Perfecto
    SciELO Espana/Repisalud

  • Periodontitis in diabetes and copaiba as a coadjuvant of the treatment: Case reports
    D Ramos-Perfecto, L Maita Véliz, L Mariano Maita Castañeda, A Castro Luna, and J Eleodoro Villavicencio Gastelú
    SciELO Espana/Repisalud

  • A natural product of possible support for the treatment of periodontitis: A literature review


  • Antibacterial and antioxidant activity of ethanolic extract from leaves of prosopis pallida leaves (American carob)