Association between periodontal pathogens and Alzheimer’s disease Avances En Odontoestomatologia, 2025
EFFECT OF ADDING FLUORIDE TO CAMELLIA SINENSIS AGAINST DENTIN EROSION GENERATED BY NON-BACTERIAL ACIDS Facultad de Odontología, Universidad Nacional Mayor de San Marcos, Lima, Perú., Ricardo Reyes-Mansilla, Donald Ramos-Perfecto, Facultad de Odontología, Universidad Nacional Mayor de San Marcos, Lima, Perú., Luis Maita-Véliz, et al. Journal of Oral Research, 2024 Introduction: Natural products are an option to be used in different conditions in the oral cavity, such as Camellia sinensis, which due to its different properties would be beneficial in the erosion of the dental surface. Objetive: Determinar el efecto del extracto de Camellia sinensis (C. sinensis) adicionado con flúor frente a la acción erosiva de ácidos no bacterianos en la superficie de la dentina humana Materials and Methods: Quasi-experimental, prospective, longitudinal, and in vitro study, consisting of 50 samples of human dentin, which were classified into 5 study groups: distilled water, hydrochloric acid, 2% C. sinensis extract, sodium fluoride. 2% and 2% C. sinensis extract added with 2% sodium fluoride. The surface roughness of each sample was evaluated with the SRT6200 digital roughness meter, obtaining a total average roughness and the measurements were carried out in two moments. Results: It was observed that the 2% C. sinensis extract, the 2% sodium fluoride, and the C. sinensis extract added with 2% sodium fluoride, showed a variation in surface roughness between before and after, which was not significant (p<0.05) in all cases. It should be noted that the C. sinensis plus sodium fluoride group is the one that obtained the best variation in the mean surface roughness than the other groups studied. Conclusions: The 2% C. sinensis extract, 2% sodium fluoride, and the combination of both compounds demonstrated an inhibitory effect against the erosive action of hydrochloric acid (0.01 M) on the dentin surface, not presenting a statistically significant difference in the results.
Increasing Analytical Quality by Designing a Thin-Layer Chromatography Scanner Method for the Determination of the Radiochemical Purity of Radiopharmaceutical Sodium Iodide 131I Oral Solution Miguel Vasquez-Huaman, Américo Castro-Luna, Norma Julia Ramos-Cevallos, Donald Ramos-Perfecto, Mario Alcarraz-Curi, Jacqueline Segura-Vasquez, Danny Cáceres-Antaurco Molecules, 2024 The goal of this study was to apply the principles of analytical quality by design (AQbD) to the analytical method for determining the radiochemical purity (PQR) of the radiopharmaceutical sodium iodide 131I oral solution, utilizing thin-layer chromatography (TLC) with a radio–TLC scanner, which also enables the evaluation of product quality. For AQbD, the analytical target profile (ATP), critical quality attributes (CQA), risk management, and the method operable design region (MODR) were defined through response surface methodology to optimize the method using MINITAB® 19 software. This study encompassed the establishment of a control strategy and the validation of the method, including the assessment of selectivity, linearity, precision, robustness, detection limit, quantification limit, range, and the stability of the sample solution. Under the experimental conditions, the method parameters of the TLC scanner were experimentally demonstrated and optimized with an injection volume of 3 µL, a radioactive concentration of 10 mCi/mL, and a carrier volume of 40 µL. Statistical analysis confirmed the method’s selectivity for the 131I iodide band Rf of 0.8, a radiochemical impurity IO3− Rf of 0.6, a linearity from 6.0 to 22.0 mCi/mL, and an intermediate precision with a global relative standard deviation (RSD) of 0.624%. The method also exhibited robustness, with a global RSD of 0.101%, a detection limit of 0.09 mCi/mL, and a quantification limit of 0.53 Ci/mL, meeting the prescribed range and displaying stability over time (at 0, 2, and 20 h) with a global RSD of 0.362%, resulting in consistent outcomes. The development of a method based on AQbD facilitated the creation of a design space and an operational space, with comprehensive knowledge of the method’s characteristics and limitations. Additionally, throughout all operations, compliance with the acceptance criteria was verified. The method’s validity was confirmed under the established conditions, making it suitable for use in the manufacturing process of sodium iodide 131I and application in nuclear medicine services.
CAMELLIA SINENSIS, A NATURAL PRODUCT TO SUPPORT THE TREATMENT OF MEDICAL AND STOMATOLOGICAL CONDITIONS Ricardo Reyes-Mansilla, , Adelmo Cuentas-Robles, Donald Ramos-Perfecto, , and Journal of Oral Research, 2023 Introduction: The tea plant, Camellia sinensis, is one of the most popular non-alcoholic beverages in the world. The main components of Camellia sinensis include amino acids, fatty acids, phenolic compounds, flavins and purine alkaloids (xanthines). For this reason, in the field of medicine, Camellia sinensis has been used as an anticancer, anxiolytic, antidiabetic, antiobesity, anti-inflammatory, analgesic, antipyretic, chemopreventive, cytotoxic and apoptogenic, genoprotective, hepatoprotective, nephroprotective, hemato-protective, and in wound healing, among other uses. Objective: To carry out a systematic review of the use of C. sinensis as supportive therapy in the treatment of oral disorders. Materials and Methods: This systematic review was carried out following the PRISMA guidelines. The search was carried out in the PubMed, ScienceDirect and Google Academic databases. Articles from studies of Camellia sinensis were reviewed and those from a secondary source, such as literature review articles, were excluded Results: A total of 12 full-text articles were selected for review, in which the properties of Camellia sinensis are detailed. Conclusions: According to the bibliography reviewed, C. sinensis exhibits anticariogenic properties, applications in the treatment of dental erosion, applications in the treatment of gingivitis and bacterial plaque, and applications in the prevention of oral cancer; however, more controlled clinical trials are needed to confirm its effectiveness and safety of use. Keywords: Camellia sinensis; Botany; Functional claim; Therapeutics; Dentistry; Systematic Review.
Art and science in the diagnosis of atypical periodontal disease: Case report LV Maita Véliz, M Castañeda Mosto, LM Maita Castañeda, U Rivas Almonte, D Ramos Perfecto Avances En Odontoestomatologia, 2021 Las lesiones pulpares y periodontales son las más prevalentes en la cavidad bucal y, tal vez por ello, son las causas más frecuentes de consulta odontológica en nuestro medio y en el mundo. Estas patologÃas tienen principalmente, etiologÃa bacteriana y una flora polimicrobiana. Las estructuras anatómicas e histológicas de los tejidos periodontales y pulpares presentan interrelaciones, que deben considerarse en la etiopatologÃa de sus procesos. El diagnóstico de las lesiones endoperiodontales no siempre se puede definir claramente, lo cual crea vacÃos que dificultan el diagnóstico de las enfermedades pulpares y periodontales. El odontólogo debe estar preparado y entrenado para que le sea posible definir el diagnóstico diferencial de las dos enfermedades y brindar un tratamiento eficiente. Se reporta un caso clÃnico, donde el manejo del examen clÃnico, asà como de la anatomÃa endoperiodontal, son decisivos para llegar al diagnóstico correcto y el tratamiento oportuno y adecuado.
A natural product of possible support for the treatment of periodontitis: A literature review Avances En Odontoestomatologia, 2020
Antibacterial and antioxidant activity of ethanolic extract from leaves of prosopis pallida leaves (American carob) Revista Cubana De Plantas Medicinales, 2017
