Fajar Husen

Verified email at gmail.com

Medical Laboratory of Technology
College of Health Science of Bina Cipta Husada



                                                     

https://researchid.co/fajarhusen

Lecturer of Medical Laboratory of Technology

EDUCATION

Bachelor Degree of Biology of Jenderal Soedirman University (UNSOED)
Master of Science of Biology (Microbiology) of Jenderal Soedirman University (UNSOED)

RESEARCH INTERESTS

Microbiology, Immunology, Medical Mycology, Histology

FUTURE PROJECTS

SOYBEAN SELECTION AGAINST CERCOSPORA LEAF BLIGHT DISEASE CAUSED BY CERCOSPORA KIKUCHII BASED ON ANATOMICAL RESISTANCE

Soybean (Glycine max L. Merr.) is the third food crop commodity after rice and maize in Indonesia. This plant is also known as the most important source of vegetable protein, which is relatively inexpensive, but a decrease in soybean productivity can occur due to infection with disease-causing pathogens, one of is Cercospora kikuchii which causes Cercospora leaf blight (CLB). The research objectives were to determine the anatomical resistance and disease severity of soybean cultivars against CLB. The method was an experiment with a completely randomized design (CRD) factorial pattern; factor 1 being soybean cultivars (Dering, Slamet, Grobogan, Wilis) and factor 2, namely pathogen inoculation (0 conidiospores/mL and 105 conidiospores/mL). Anatomical method preparations using paraffin, staining with 1% safranin. Disease criteria are based on the council of scientific and industrial research (CSIR) assessment method. Data were analysis used analysis of variance (p<0.05)


Applications Invited
Anatomical resistance

Ethanol extract of the mushroom Coprinus comatus exhibits anti-diabetic and antioxidant activities in streptozotocininduced diabetic rats

To identify the benefits of ethanol extracts of the C. comatus fruit body in streptozotocin-induced hyperglycemic rats by evaluating their blood glucose, glycosylated hemoglobin (HbA1c), insulin, glucagon-like peptide-1 (GLP-1), dipeptidyl peptidase-4 (DPP-4), and glutathione (GSH) levels, with and without extract administration


Applications Invited
Antidiabetic effect

Antioxidant Activities and Properties of Coprinus comatus Mushroom Both Mycelium and Fruiting Body Extracts In Streptozotocin-Induced Hyperglycemic Rats Model

The study evaluated in vitro and in vivo treatments to evaluate antioxidant effect and activity of C. comatus. The in vivo treatment was conducted using six groups of Wistar rats (n = 24). Group 1 healthy control (HC), groups 2–6 received 45 mg/Kg BW of streptozotocin once, group 2 just streptozotocin-induced (NC), group 3 was given 45 mg/kg BW of metformin (PC), groups 4–6 were given 250 (T1), 500 (T2), and 750 mg (T3) of C. comatus extract for 14 days, and the in vitro was conducted using an antioxidant oxidant assay. Data were analyzed using analysis of variance and Duncan’s multiple-range tests


Applications Invited
Antioxidant supplement
3

Scopus Publications

11

Scholar Citations

1

Scholar h-index

Scopus Publications

  • Ethanol extract of the mushroom Coprinus comatus exhibits antidiabetic and antioxidant activities in streptozotocin-induced diabetic rats
    N. I. Ratnaningtyas, Hernayanti Hernayanti, Nuraeni Ekowati and F. Husen

    Pharmaceutical Biology, ISSN: 13880209, eISSN: 17445116, Pages: 1126-1136, Published: 2022
    Abstract Context Edible mushrooms have a long history of use in traditional Chinese or Japanese medicine. Coprinus comatus (O.F. Müll.) Pers. (Agaricaceae) contains antioxidant and antidiabetic agents. Objective To identify the benefits of ethanol extracts of the C. comatus fruit body in streptozotocin-induced hyperglycaemic rats by evaluating their blood glucose, glycosylated haemoglobin (HbA1c), insulin, glucagon-like peptide-1 (GLP-1), dipeptidyl peptidase-4 (DPP-4), and glutathione (GSH) levels, with and without extract administration. Materials and methods Wistar rats were either left untreated or were administered 45 mg/kg body weight (BW) streptozotocin; 45 mg/kg BW metformin; or 250, 500, or 750 mg/kg BW extract for 14 days. The blood glucose, GLP-1, DPP-4, GSH, insulin, and HbA1c levels were determined. Data were analysed using analysis of variance and Duncan’s multiple range tests. Results Preliminary data showed that administration of C. comatus ethanol extract dose of 250, 500, and 750 mg orally has no toxicity effects after 24 h administration. The ethanolic extract of fruiting body of C. comatus considerably reduced the rat’s fasting blood glucose levels 26.69%, and DPP-4 6.97% at dose of 750 mg. The extract reduced HbA1c 4–4.30%, increased GLP-1 71.09%, GSH 11.19% at dose of 500 mg, and increased insulin levels 13.83%. Extracts contain bioactive compounds such as flavonoid, alkaloid, terpenoids, vitamins C and E, rutin, and saponin. Conclusions The C. comatus extract can be used as herbal medicine that reduces diabetic symptoms. Further investigation on C. comatus extracts should be conducted with gas chromatography-mass spectrometry to characterise the bioactive compounds.

  • Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
    N. I. Ratnaningtyas, Hernayanti, N. Ekowati and F. Husen

    IOP Conference Series: Earth and Environmental Science, ISSN: 17551307, eISSN: 17551315, Volume: 948, Published: 16 December 2021
    Diabetes mellitus (DM) is a metabolic disease with an increasing prevalence. An increase in free radicals and AGEs can cause glomerular cell damage which can lead to inflammation and DM nephropathy. WHO has recommended DM treatment by using herbal medicines that have minimal side effects and have a lot of biological activity to prevent complications. Coprinus comatus (O.F. Mull.) is known as immunomodulatory, anti-inflammatory and antidiabetic agent. The research used C. comatus cultivated in Cianjur. The basidiome of this mushroom has slightly oval and small with height of 8-12 cm and thickness of 2-3 cm. The research included six groups of male Wistar rats: Group 1 received no treatment, Groups 2–6 were administered 45 mg/kg BW streptozotocin once, Group 3 was administered 45 mg/kg BW metformin, Groups 4, 5, and 6 were administered 250, 500, and 750 mg/kg BW of C. comatus fruit body ethanol extract respectively for 14 days. The superoxide dismutase (SOD), malondialdehyde (MDA), hepcidin and β2 microglobulin (B2M) levels were evaluated. Data were analyzed using analysis of variance and Duncan’s multiple-range tests. The results showed that dose of 500 mg was effective in increasing SOD and decreasing hepcidin, B2M and MDA levels.

  • Antidiabetic effects and antioxidant properties of the saggy ink cap medicinal mushroom, coprinus comatus (Agaricomycetes), in streptozotocin-induced hyperglycemic rats
    Fajar Husen, Hernayanti Hernayanti, Nuraeni Ekowati, Dalia Sukmawati, and Nuniek Ina Ratnaningtyas

    International Journal of Medicinal Mushrooms, ISSN: 15219437, eISSN: 19404344, Pages: 9-21, Published: 2021 Begell House
    Coprinus comatus is known for its antihyperglycemic benefits. This study aimed to identify the effect of bioactive compounds of C. comatus extract as an antidiabetic agent linked to glucagon-like peptide 1 (GLP-1) and antioxidant properties in increasing glutathione (GSH) levels. This study used six groups of Wistar rats (n = 24). Group 1 comprised the healthy control. Groups 2-6 received 45 mg of streptozotocin/kg body weight (BW) once. Group 3 was also given 45 mg of metformin/kg BW, whereas groups 4-6 were also given 250, 500, and 750 mg of C. comatus ethyl acetate extract/kg BW for 14 days. Antidiabetic effects of alkaloids and saponin were seen in blood glucose and glycated hemoglobin (HbA1c) degradation, increased insulin, and increased inhibition of GLP-1 through dipeptidyl peptidase-4 activity. Flavonoid antioxidants, ascorbic acid (vitamin C), and α-tocopherol (vitamin E) are useful in protecting pancreatic в cells from free radicals. Data were analyzed using analysis of variance and Duncan's multiple range test. C. comatus ethyl acetate extract at doses of 250, 500, and 750 mg/kg BW worked as an antidiabetic and antioxidant agent that contained flavonoids (16.4 mg/L), alkaloids (2.97 mg/L), saponin, rutin (351.13 ppm), vitamin C (132.342 mg/L), and vitamin E (102.320 g/L). The 250-mg dose was effective in increasing insulin (8.11 mlU/mL) and reducing blood glucose (23.92%) and HbA1c (3.775%), whereas the 500-mg dose was effective in increasing levels of GLP-1 (1056.923 ng/L) and GSH (4.62 μmol/L).

RECENT SCHOLAR PUBLICATIONS

  • Ethanol extract of the mushroom Coprinus comatus exhibits antidiabetic and antioxidant activities in streptozotocin-induced diabetic rats
    NI Ratnaningtyas, H Hernayanti, N Ekowati, F Husen
    Pharmaceutical Biology 60 (1), 1126-1136 2022

  • Peningkatan Kadar Kolesterol dan Usia Pada Ibu Rumah Tangga
    F Husen, NI Ratnaningtyas, NAH Khasanah, NI Yuniati
    Jurnal Ilmiah Kesehatan Sandi Husada, 343-351 2022

  • Increased Cholesterol Levels and Age in Housewives
    F Husen, NI Ratnaningtyas, NAH Khasanah, NI Yuniati
    Jurnal Ilmiah Kesehatan Sandi Husada 11 (2), 343-351 2022

  • Analisis Faktor Risiko Personal Hygiene terhadap Pediculosis capitis pada Santriwati Ponpes Miftahul Huda
    NAHK Khasanah, NI Yuniati, F Husen, UF Rudatiningtyas
    Journal of Holistics and Health Sciences (JHHS) 4 (2), 282-291 2022

  • Inhibitory Test of Gentamicin Antibiotics Against Escherichia coli and Staphylococcus aureus Bacteria Using Disc Method
    F Husen, NI Ratnaningtyas
    Biotropika: Journal of Tropical Biology 10 (2) 2022

  • Edukasi Peningkatan Kebersihan Diri Santriwati Saat Menstruasi Di Pesantren Miftahul Huda Putri
    U Rudatiningtyas, NAH Khasanah, F Husen
    Jurnal Pengabdian Masyarakat Indonesia 2 (4), 477-482 2022

  • Sosialisasi Dan Monitoring Kebersihan Tandon Air Di Desa Mandiraja Wetan Sebagai Upaya Peningkatkan Status Kesehatan
    F Husen, NI Ratnaningtyas, NI Yuniati, NAH Khasanah, ...
    Jurnal Pengabdian Masyarakat Indonesia 2 (4), 459-464 2022

  • Karakteristik Dan Gambaran Diagnosa Komplikasi Pasien Diabetes Di Rumah Sakit Umum Aghisna Sidareja
    R Basuki, F Husen
    Jurnal Bina Cipta Husada 18 (2), 1-15 2022

  • Karakteristik, Profil Dan Diganosa Pasien Hipertensi Rawat Inap Di RSU Aghisna Sidareja Kabupaten Cilacap
    F Husen, R Basuki
    Jurnal Bina Cipta Husada 18 (2), 59-73 2022

  • Antioxidant Activities and Properties of Coprinus comatus Mushroom Both Mycelium and Fruiting Body Extracts In Streptozotocin-Induced Hyperglycemic Rats Model
    NI Ratnaningtyas, H Hernayanti, N Ekowati, F Husen, I Maulida, ...
    Biosaintifika: Journal of Biology & Biology Education 14 (1), 9-21 2022

  • Soybean Selection Against Cercospora Leaf Blight Disease Caused By Cercospora kikuchii Based on Anatomical Resistance
    F Husen, NI Ratnaningtyas, S Samiyarsih, JS Muljowati, N Fitrianto
    Biosaintifika: Journal of Biology & Biology Education 14 (1), 90-102 2022

  • AKTIVITAS ANTI-HEPATOTOKSIK DAN ANTI-NEFROTOKSIK EKSTRAK ETIL ASETAT TUBUH BUAH Coprinus comatus PADA TIKUS WISTAR JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN
    NI Ratnaningtyas, N Ekowati, F Husein, A Perdanawati
    Prosiding 11 (1) 2022

  • Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
    NI Ratnaningtyas, N Ekowati, F Husen
    IOP Conference Series: Earth and Environmental Science 948 (1), 012078 2021

  • Antidiabetic Effects and Antioxidant Properties of the Saggy Ink Cap Medicinal Mushroom, Coprinus comatus (Agaricomycetes), in Streptozotocin-Induced Hyperglycemic Rats
    F Husen, H Hernayanti, N Ekowati, D Sukmawati, NI Ratnaningtyas
    International Journal of Medicinal Mushrooms 23 (10), 9-21 2021

  • Coprinus comatus Ethyl Acetate Extract as Antidiabetic and Antioxidant Effects in Hyperglycemic Wistar Rat (Rattus norvegicus) Models
    F Husen, NI Ratnaningtyas, Hernayanti
    Jenderal Soedirman University, Purwokerto 2019

  • Uji Ketahanan Struktural Daun Kedelai (glycine max (l.) Merr.) Kultivar Tahan dan Rentan terhadap Penyakit Bercak Daun
    F HUSEN
    Universitas Jenderal Soedirman 2017

  • Structural Resistance Test of Soybean Leaves (Glycine max (L.) Merr.) Cultivars Resistant and Susceptible to Leaf Spot Disease
    F Husen
    Universitas Jenderal Soedirman (UNSOED) 2017

MOST CITED SCHOLAR PUBLICATIONS

  • Antidiabetic Effects and Antioxidant Properties of the Saggy Ink Cap Medicinal Mushroom, Coprinus comatus (Agaricomycetes), in Streptozotocin-Induced Hyperglycemic Rats
    F Husen, H Hernayanti, N Ekowati, D Sukmawati, NI Ratnaningtyas
    International Journal of Medicinal Mushrooms 23 (10), 9-21 2021
    Citations: 6

  • Ethanol extract of the mushroom Coprinus comatus exhibits antidiabetic and antioxidant activities in streptozotocin-induced diabetic rats
    NI Ratnaningtyas, H Hernayanti, N Ekowati, F Husen
    Pharmaceutical Biology 60 (1), 1126-1136 2022
    Citations: 1

  • Karakteristik Dan Gambaran Diagnosa Komplikasi Pasien Diabetes Di Rumah Sakit Umum Aghisna Sidareja
    R Basuki, F Husen
    Jurnal Bina Cipta Husada 18 (2), 1-15 2022
    Citations: 1

  • Karakteristik, Profil Dan Diganosa Pasien Hipertensi Rawat Inap Di RSU Aghisna Sidareja Kabupaten Cilacap
    F Husen, R Basuki
    Jurnal Bina Cipta Husada 18 (2), 59-73 2022
    Citations: 1

  • Soybean Selection Against Cercospora Leaf Blight Disease Caused By Cercospora kikuchii Based on Anatomical Resistance
    F Husen, NI Ratnaningtyas, S Samiyarsih, JS Muljowati, N Fitrianto
    Biosaintifika: Journal of Biology & Biology Education 14 (1), 90-102 2022
    Citations: 1

  • Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
    NI Ratnaningtyas, N Ekowati, F Husen
    IOP Conference Series: Earth and Environmental Science 948 (1), 012078 2021
    Citations: 1

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

Effervescent of Mushroom Extracts