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Medical Laboratory of Technology
College of Health Science of Bina Cipta Husada
Lecturer of Medical Laboratory of Technology
Bachelor Degree of Biology of Jenderal Soedirman University (UNSOED)
Master of Science of Biology (Microbiology) of Jenderal Soedirman University (UNSOED)
Microbiology, Immunology, Medical Mycology, Histology
Soybean (Glycine max L. Merr.) is the third food crop commodity after rice and maize in Indonesia. This plant is also known as the most important source of vegetable protein, which is relatively inexpensive, but a decrease in soybean productivity can occur due to infection with disease-causing pathogens, one of is Cercospora kikuchii which causes Cercospora leaf blight (CLB). The research objectives were to determine the anatomical resistance and disease severity of soybean cultivars against CLB. The method was an experiment with a completely randomized design (CRD) factorial pattern; factor 1 being soybean cultivars (Dering, Slamet, Grobogan, Wilis) and factor 2, namely pathogen inoculation (0 conidiospores/mL and 105 conidiospores/mL). Anatomical method preparations using paraffin, staining with 1% safranin. Disease criteria are based on the council of scientific and industrial research (CSIR) assessment method. Data were analysis used analysis of variance (p<0.05)
To identify the benefits of ethanol extracts of the C. comatus fruit body in streptozotocin-induced hyperglycemic rats by evaluating their blood glucose, glycosylated hemoglobin (HbA1c), insulin, glucagon-like peptide-1 (GLP-1), dipeptidyl peptidase-4 (DPP-4), and glutathione (GSH) levels, with and without extract administration
The study evaluated in vitro and in vivo treatments to evaluate antioxidant effect and activity of C. comatus. The in vivo treatment was conducted using six groups of Wistar rats (n = 24). Group 1 healthy control (HC), groups 2–6 received 45 mg/Kg BW of streptozotocin once, group 2 just streptozotocin-induced (NC), group 3 was given 45 mg/kg BW of metformin (PC), groups 4–6 were given 250 (T1), 500 (T2), and 750 mg (T3) of C. comatus extract for 14 days, and the in vitro was conducted using an antioxidant oxidant assay. Data were analyzed using analysis of variance and Duncan’s multiple-range tests
N. I. Ratnaningtyas, Hernayanti Hernayanti, Nuraeni Ekowati and F. Husen
Pharmaceutical Biology, ISSN: 13880209, eISSN: 17445116, Pages: 1126-1136, Published: 2022
Abstract Context Edible mushrooms have a long history of use in traditional Chinese or Japanese medicine. Coprinus comatus (O.F. Müll.) Pers. (Agaricaceae) contains antioxidant and antidiabetic agents. Objective To identify the benefits of ethanol extracts of the C. comatus fruit body in streptozotocin-induced hyperglycaemic rats by evaluating their blood glucose, glycosylated haemoglobin (HbA1c), insulin, glucagon-like peptide-1 (GLP-1), dipeptidyl peptidase-4 (DPP-4), and glutathione (GSH) levels, with and without extract administration. Materials and methods Wistar rats were either left untreated or were administered 45 mg/kg body weight (BW) streptozotocin; 45 mg/kg BW metformin; or 250, 500, or 750 mg/kg BW extract for 14 days. The blood glucose, GLP-1, DPP-4, GSH, insulin, and HbA1c levels were determined. Data were analysed using analysis of variance and Duncan’s multiple range tests. Results Preliminary data showed that administration of C. comatus ethanol extract dose of 250, 500, and 750 mg orally has no toxicity effects after 24 h administration. The ethanolic extract of fruiting body of C. comatus considerably reduced the rat’s fasting blood glucose levels 26.69%, and DPP-4 6.97% at dose of 750 mg. The extract reduced HbA1c 4–4.30%, increased GLP-1 71.09%, GSH 11.19% at dose of 500 mg, and increased insulin levels 13.83%. Extracts contain bioactive compounds such as flavonoid, alkaloid, terpenoids, vitamins C and E, rutin, and saponin. Conclusions The C. comatus extract can be used as herbal medicine that reduces diabetic symptoms. Further investigation on C. comatus extracts should be conducted with gas chromatography-mass spectrometry to characterise the bioactive compounds.
N. I. Ratnaningtyas, Hernayanti, N. Ekowati and F. Husen
IOP Conference Series: Earth and Environmental Science, ISSN: 17551307, eISSN: 17551315, Volume: 948, Published: 16 December 2021
Diabetes mellitus (DM) is a metabolic disease with an increasing prevalence. An increase in free radicals and AGEs can cause glomerular cell damage which can lead to inflammation and DM nephropathy. WHO has recommended DM treatment by using herbal medicines that have minimal side effects and have a lot of biological activity to prevent complications. Coprinus comatus (O.F. Mull.) is known as immunomodulatory, anti-inflammatory and antidiabetic agent. The research used C. comatus cultivated in Cianjur. The basidiome of this mushroom has slightly oval and small with height of 8-12 cm and thickness of 2-3 cm. The research included six groups of male Wistar rats: Group 1 received no treatment, Groups 2–6 were administered 45 mg/kg BW streptozotocin once, Group 3 was administered 45 mg/kg BW metformin, Groups 4, 5, and 6 were administered 250, 500, and 750 mg/kg BW of C. comatus fruit body ethanol extract respectively for 14 days. The superoxide dismutase (SOD), malondialdehyde (MDA), hepcidin and β2 microglobulin (B2M) levels were evaluated. Data were analyzed using analysis of variance and Duncan’s multiple-range tests. The results showed that dose of 500 mg was effective in increasing SOD and decreasing hepcidin, B2M and MDA levels.
Fajar Husen, Hernayanti Hernayanti, Nuraeni Ekowati, Dalia Sukmawati, and Nuniek Ina Ratnaningtyas
International Journal of Medicinal Mushrooms, ISSN: 15219437, eISSN: 19404344, Pages: 9-21, Published: 2021 Begell House
Coprinus comatus is known for its antihyperglycemic benefits. This study aimed to identify the effect of bioactive compounds of C. comatus extract as an antidiabetic agent linked to glucagon-like peptide 1 (GLP-1) and antioxidant properties in increasing glutathione (GSH) levels. This study used six groups of Wistar rats (n = 24). Group 1 comprised the healthy control. Groups 2-6 received 45 mg of streptozotocin/kg body weight (BW) once. Group 3 was also given 45 mg of metformin/kg BW, whereas groups 4-6 were also given 250, 500, and 750 mg of C. comatus ethyl acetate extract/kg BW for 14 days. Antidiabetic effects of alkaloids and saponin were seen in blood glucose and glycated hemoglobin (HbA1c) degradation, increased insulin, and increased inhibition of GLP-1 through dipeptidyl peptidase-4 activity. Flavonoid antioxidants, ascorbic acid (vitamin C), and α-tocopherol (vitamin E) are useful in protecting pancreatic в cells from free radicals. Data were analyzed using analysis of variance and Duncan's multiple range test. C. comatus ethyl acetate extract at doses of 250, 500, and 750 mg/kg BW worked as an antidiabetic and antioxidant agent that contained flavonoids (16.4 mg/L), alkaloids (2.97 mg/L), saponin, rutin (351.13 ppm), vitamin C (132.342 mg/L), and vitamin E (102.320 g/L). The 250-mg dose was effective in increasing insulin (8.11 mlU/mL) and reducing blood glucose (23.92%) and HbA1c (3.775%), whereas the 500-mg dose was effective in increasing levels of GLP-1 (1056.923 ng/L) and GSH (4.62 μmol/L).
Effervescent of Mushroom Extracts