NIR-activated catechol-functionalized nanodiamond nanofibers for accelerating on-demand MRSA and E. coli biofilm eradication Hyeonseo Park, Tejal V. Patil, Jieun Lee, Hojin Kim, Seong-Jun Cho, et al. Journal of Biological Engineering, 2025 The rise of antibiotic resistance has made bacterial infections a persistent global health issue. In particular, extracellular polymeric substances (EPS) secreted by bacteria limit the effectiveness of conventional antibiotics, making biofilm removal challenging. To address this, we created ND@PDA nanoparticles by coating the surface of nanodiamonds (ND) with polydopamine (PDA). These nanoparticles were then integrated into polyvinyl alcohol to fabricate PVA/ND@PDA nanofiber scaffolds, resulting in an innovative platform with enhanced photothermal, antibacterial and antibiofilm properties. Upon exposure to near-infrared (NIR) light, the scaffolds exhibited a significant photothermal activity, oxidative stress and effectively damaging key bacterial components, such as biofilm, bacterial membranes, and proteins. Additionally, the catechol groups in PDA provided strong cell adhesion and high biocompatibility on the nanofiber surface. Our research proposes a platform that not only effectively addresses antibiotic-resistant infections but also contributes to advancements in wound healing therapies by enabling controlled antibacterial action with minimal toxicity.
Electroconductive Nanocellulose, a Versatile Hydrogel Platform: From Preparation to Biomedical Engineering Applications Myoung Joon Jeon, Aayushi Randhawa, Hojin Kim, Sayan Deb Dutta, Keya Ganguly, et al. Advanced Healthcare Materials, 2025 Nanocelluloses have garnered significant attention recently in the attempt to create sustainable, improved functional materials. Nanocellulose possesses wide varieties, including rod‐shaped crystalline cellulose nanocrystals and elongated cellulose nanofibers, also known as microfibrillated cellulose. In recent times, nanocellulose has sparked research into a wide range of biomedical applications, which vary from developing 3D printed hydrogel to preparing structures with tunable characteristics. Owing to its multifunctional properties, different categories of nanocellulose, such as cellulose nanocrystals, cellulose nanofibers, and bacterial nanocellulose, as well as their unique properties are discussed here. Here, different methods of nanocellulose‐based hydrogel preparation are covered, which include 3D printing and crosslinking methods. Subsequently, advanced nanocellulose‐hydrogels addressing conductivity, shape memory, adhesion, and structural color are highlighted. Finally, the application of nanocellulose‐based hydrogel in biomedical applications is explored here. In summary, numerous perspectives on novel approaches based on nanocellulose‐based research are presented here.
In Vitro Osteogenic Response to Copper-Doped Eggshell-Derived Hyroxyapatite With Macrophage Supplements Tejal V. Patil, Dinesh K. Patel, Ki‐Taek Lim Journal of Biomedical Materials Research Part A, 2025 The high bioactivity and biocompatibility of hydroxyapatite (HAP) make it a useful bone graft material for bone tissue engineering. However, the development superior osteoconductive and osteoinductive materials for bone regeneration remains a challenge. To overcome these constraints, Cu‐doped hydroxyapatite (HAP(Cu)) from waste eggshells has been produced for bone tissue engineering. The materials produced were characterized using Fourier transform infrared spectroscopy, x‐ray diffraction, and photoelectron spectroscopy. The scanning microscopy images revealed that the developed HAP was a rod‐like crystalline structure with a typical 80–150 nm diameter. Energy‐dispersive x‐ray spectroscopy showed that the generated HAP was mostly composed of calcium, oxygen, and phosphorus. The Ca/P molar ratios in eggshell‐derived and copper‐doped HAP were 1.61 and 1.67, respectively, similar to the commercially available HAP ratio (1.67). The WST‐8 assay was used to assess the biocompatibility of HAPs with hBMSCs. HAP(Cu) in the media significantly altered the cytotoxicity of biocompatible HAP(Cu). The osteogenic potential of HAP(Cu) was demonstrated by greater mineralization than that of pure HAP or the control. HAP(Cu) showed higher osteogenic gene expression than pure HAP and the control, indicating its stronger osteogenic potential. Furthermore, we assessed the effects of sample‐treated macrophage‐derived conditioned medium (CM) on hBMSCs' osteogenesis. CM‐treated HAP(Cu) demonstrated a significantly higher osteogenic potential vis‐à‐vis pure HAP(Cu). These findings revealed that HAP(Cu) with CM significantly improved osteogenesis in hBMSCs and can be explored as a bone graft in bone tissue engineering.
Can CRISPRized crops save the global food supply? Kamel A. Abd-Elsalam, Ki-Taek Lim Crispr and Rnai Systems Nanobiotechnology Approaches to Plant Breeding and Protection A Volume in Nanobiotechnology for Plant Protection, 2021
Preface Kamel A. Abd-Elsalam, Ki-Taek Lim Crispr and Rnai Systems Nanobiotechnology Approaches to Plant Breeding and Protection A Volume in Nanobiotechnology for Plant Protection, 2021
Silver nanoparticles for wastewater treatment Keya Ganguly, Sayan Deb Dutta, Dinesh K. Patel, Ki-Taek Lim Aquananotechnology Applications of Nanomaterials for Water Purification, 2020
A fully automated bioreactor system for precise control of stem cell proliferation and differentiation 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2019, 2019
Pulsed-electromagnetic field-assisted reduced graphene oxide substrates for multidifferentiation of human mesenchymal stem cells 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2018, 2018
Effects of micro/nano-sized natural calcium phosphate cements on behaviors of alveolar bone marrow stem cells American Society of Agricultural and Biological Engineers Annual International Meeting 2014 Asabe 2014, 2014
Development of fast-hardening calcium phosphate cements using micro/nano horse bone powders American Society of Agricultural and Biological Engineers Annual International Meeting 2013 Asabe 2013, 2013
Noninvasive measurement of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells using near-infrared spectroscopy Tissue Engineering and Regenerative Medicine, 2011
Molecular responses in osteogenic differentiation of mesenchymal stem cells induced by physical stimulation Tissue Engineering and Regenerative Medicine, 2011
Novel composite scaffolds for tooth regeneration using human dental pulp stem cells Tissue Engineering and Regenerative Medicine, 2010
Evaluation of novel degradable poly-bioceramc scaffolds of PDLLA/toothapatite/TCP using human dental pulp stem cells for tooth bioengineering Tissue Engineering and Regenerative Medicine, 2010
Development of a perfusion bioreactor system for alveolar bone tissue engineering American Society of Agricultural and Biological Engineers Annual International Meeting 2009 Asabe 2009, 2009
Effects of low intensity ultrasound stimulation on the proliferation of alveolar bone marrow stem cells American Society of Agricultural and Biological Engineers Annual International Meeting 2009 Asabe 2009, 2009
Development of nano-matrix composed of Poly(ε-caprolactone) and polyethylenimine using electrospinning system for tissue engineering American Society of Agricultural and Biological Engineers Annual International Meeting 2009 Asabe 2009, 2009
Biomechanical effects of fluid dynamics and mass transport on cell growth in perfusion bioreactors for tissue engineering Tissue Engineering and Regenerative Medicine, 2009
Development of a perfusion flow bioreactor system for culturing human alveolar bone marrow stem cells Tissue Engineering and Regenerative Medicine, 2009
In vitro effects of electromagnetic field stimulation on cells in tissue engineering Tissue Engineering and Regenerative Medicine, 2009
Influence of static magnetic field stimulation on cells for tissue engineering Tissue Engineering and Regenerative Medicine, 2009
Mechanical stimulation of mesenchymal stem cells for tissue engineering Tissue Engineering and Regenerative Medicine, 2009
Novel perfusion bioreactor systems for tissue engineering Tissue Engineering and Regenerative Medicine, 2009
Effects of low intensity ultrasound stimulation on the proliferation of alveolar bone marrow stem cell Tissue Engineering and Regenerative Medicine, 2008
Development of artificial eardrum patch to repair tympanic membrane perforations American Society of Agricultural and Biological Engineers Annual International Meeting 2008 Asabe 2008, 2008
RECENT SCHOLAR PUBLICATIONS
Electrically stimulated asymmetric double-layer scaffolds using 3D printing and electrospinning for enhanced bacteria-free wound healing application J Lee, A Randhawa, H Park, H Kim, SJ Cho, KT Lim Journal of Nanobiotechnology 24 (1), 233 , 2026 2026 Citations: 2
Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose SD Dutta, JM An, J Mondal, S Bose, SK Jana, Y Seol, CK Maity, Y Lee, ... Bioactive Materials 62, 883-898 , 2026 2026
3D-printed nanoengineered bioadhesive with tunable stiffness for uncovering electromechanotherapy-assisted cardiac microenvironment SD Dutta, JM An, TV Patil, H Kim, TS Santra, Y Lee, KT Lim Biomaterials, 124175 , 2026 2026
Corrigendum to “Skin-inspired phototherapeutic cryogel ameliorates infected wound healing by orchestrating mechanotransduction and immunomodulation”[Bioact. Mater. 57 (2026 … SD Dutta, JM An, M Moniruzzaman, R Acharya, Y Seol, H Kim, ... Bioactive Materials 58, 183 , 2026 2026
Skin-inspired phototherapeutic cryogel ameliorates infected wound healing by orchestrating mechanotransduction and immunomodulation SD Dutta, JM An, M Moniruzzaman, R Acharya, Y Seol, H Kim, ... Bioactive Materials 57, 768-790 , 2026 2026 Citations: 4
3D‐Printed Piezoionic/Bioelectronic Hydrogel for Electro‐Metabolic Regulation of Osteogenic Differentiation SD Dutta, MJ Jeon, Y Seol, KT Lim Advanced Healthcare Materials, e71263 , 2026 2026
Spatiotemporally activated gelatin/unzipped carbon nanotubes/chitosan-based conductive hydrogel via dual stimuli for robust methicillin-resistant Staphylococcus aureus biofilm … TV Patil, A Randhawa, H Park, R Acharya, SD Dutta, KT Lim International Journal of Biological Macromolecules, 149845 , 2025 2025
3D‐printed magnesium/nanodiamond dual‐doped hydroxyapatite composite hydrogels with antibacterial and in vitro bioactive properties for bone tissue engineering R Acharya, SD Dutta, TV Patil, H Kim, M Jeon, Y Seol, A Randhawa, ... Journal of the American Ceramic Society 108 (12), e70121 , 2025 2025 Citations: 4
Fluid Shear‐Controlled Pro/Anti‐Inflammatory Osteomodulatory Construct for Drug‐Free Immune Activation Through Cationic Ion Channel Activation K Ganguly, A Randhawa, SD Dutta, J Lee, Y Seol, ... Advanced Healthcare Materials 14 (29), e00404 , 2025 2025 Citations: 2
Gelatin as a Biomaterial for Tissue Engineering A Randhawa, K Ganguly, KT Lim Biopolymer Based Composites for Regenerative Medicines and Tissue … , 2025 2025
Bioinspired Shape Reconfigurable, Printable, and Conductive “E‐Skin” Patch with Robust Antibacterial Properties for Human Health Sensing (Adv. Funct. Mater. 40/2025) H Kim, SD Dutta, MJ Jeon, J Lee, H Park, Y Seol, KT Lim Advanced Functional Materials 35 (40), e71678 , 2025 2025 Citations: 1
Bioinspired Shape Reconfigurable, Printable, and Conductive “E‐Skin” Patch with Robust Antibacterial Properties for Human Health Sensing H Kim, SD Dutta, MJ Jeon, J Lee, H Park, Y Seol, KT Lim Advanced Functional Materials 35 (40), 2504088 , 2025 2025 Citations: 28
Ultrathin, stimuli-responsive, antimicrobial, self-cleaning, reusable, and biodegradable, micro/nanofibrous electrospun mat as an efficient face mask filter for airborne … K Ganguly, A Randhawa, SD Dutta, H Park, M Mohammad Hossein Pour, ... Nano Letters 25 (19), 7641-7650 , 2025 2025 Citations: 6
Zinc oxide@ tetracycline spiky microparticles design for persistent antibacterial therapy Y Seol, K Ganguly, TV Patil, SD Dutta, H Park, J Lee, A Randhawa, H Kim, ... Journal of Biomedical Materials Research Part A 113 (5), e37915 , 2025 2025 Citations: 2
Tailoring osteoimmunity and hemostasis using 3D-Printed nano-photocatalytic bactericidal scaffold for augmented bone regeneration SD Dutta, J Hexiu, M Moniruzzaman, TV Patil, R Acharya, JS Kim, KT Lim Biomaterials 316, 122991 , 2025 2025 Citations: 25
Nanodiamond: a multifaceted exploration of electrospun nanofibers for antibacterial and wound healing applications H Park, TV Patil, C Mo, KT Lim Journal of Nanobiotechnology 23 (1), 285 , 2025 2025 Citations: 14
Physical stimuli-responsive DNA hydrogels: design, fabrication strategies, and biomedical applications R Acharya, SD Dutta, H Mallik, TV Patil, K Ganguly, A Randhawa, H Kim, ... Journal of Nanobiotechnology 23 (1), 233 , 2025 2025 Citations: 28
3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis SD Dutta, JM An, J Hexiu, A Randhawa, K Ganguly, TV Patil, T Thambi, ... Bioactive Materials 45, 345-362 , 2025 2025 Citations: 62
NIR-activated catechol-functionalized nanodiamond nanofibers for accelerating on-demand MRSA and E. coli biofilm eradication H Park, TV Patil, J Lee, H Kim, SJ Cho, KT Lim Journal of Biological Engineering 19 (1), 2 , 2025 2025 Citations: 9
Physical stimuli-responsive 3D printable hydrogels for scalable manufacturing of bioengineered meat analogs J Lee, SD Dutta, TV Patil, SJ Cho, KT Lim Trends in Food Science & Technology 156, 104867 , 2025 2025 Citations: 6
MOST CITED SCHOLAR PUBLICATIONS
Functional cellulose-based hydrogels as extracellular matrices for tissue engineering SD Dutta, DK Patel, KT Lim Journal of biological engineering 13 (1), 55 , 2019 2019 Citations: 271
Nanocellulose, a versatile platform: From the delivery of active molecules to tissue engineering applications TV Patil, DK Patel, SD Dutta, K Ganguly, TS Santra, KT Lim Bioactive materials 9, 566-589 , 2022 2022 Citations: 260
3D-printed bioactive and biodegradable hydrogel scaffolds of alginate/gelatin/cellulose nanocrystals for tissue engineering SD Dutta, J Hexiu, DK Patel, K Ganguly, KT Lim International Journal of Biological Macromolecules 167, 644-658 , 2021 2021 Citations: 252
Bioactive effects of graphene oxide cell culture substratum on structure and function of human adipose‐derived stem cells J Kim, KS Choi, Y Kim, KT Lim, H Seonwoo, Y Park, DH Kim, PH Choung, ... Journal of Biomedical Materials Research Part A: An Official Journal of The … , 2013 2013 Citations: 208
Graphene-incorporated chitosan substrata for adhesion and differentiation of human mesenchymal stem cells J Kim, YR Kim, Y Kim, KT Lim, H Seonwoo, S Park, SP Cho, BH Hong, ... Journal of Materials Chemistry B 1 (7), 933-938 , 2013 2013 Citations: 202
Carbon nanotubes-based nanomaterials and their agricultural and biotechnological applications DK Patel, HB Kim, SD Dutta, K Ganguly, KT Lim Materials 13 (7), 1679 , 2020 2020 Citations: 182
Eggshell membrane: Review and impact on engineering S Park, KS Choi, D Lee, D Kim, KT Lim, KH Lee, H Seonwoo, J Kim Biosystems engineering 151, 446-463 , 2016 2016 Citations: 182
Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells J Kim, HN Kim, KT Lim, Y Kim, H Seonwoo, SH Park, HJ Lim, DH Kim, ... Scientific reports 3 (1), 3552 , 2013 2013 Citations: 158
Nanocellulose-based polymer hybrids and their emerging applications in biomedical engineering and water purification DK Patel, SD Dutta, KT Lim RSC advances 9 (33), 19143-19162 , 2019 2019 Citations: 146
A review of properties of nanocellulose, its synthesis, and potential in biomedical applications A Randhawa, SD Dutta, K Ganguly, TV Patil, DK Patel, KT Lim Applied Sciences 12 (14), 7090 , 2022 2022 Citations: 132
Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells J Kim, HN Kim, KT Lim, Y Kim, S Pandey, P Garg, YH Choung, ... Biomaterials 34 (30), 7257-7268 , 2013 2013 Citations: 130
Bioactive electrospun nanocomposite scaffolds of poly (lactic acid)/cellulose nanocrystals for bone tissue engineering DK Patel, SD Dutta, J Hexiu, K Ganguly, KT Lim International Journal of Biological Macromolecules 162, 1429-1441 , 2020 2020 Citations: 128
3D-printable chitosan/silk fibroin/cellulose nanoparticle scaffolds for bone regeneration via M2 macrophage polarization DK Patel, SD Dutta, J Hexiu, K Ganguly, KT Lim Carbohydrate polymers 281, 119077 , 2022 2022 Citations: 112
In Vitro Effects of Low‐Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone‐Derived Mesenchymal Stem Cells for … KT Lim, J Kim, H Seonwoo, SH Park, PH Choung, JH Chung BioMed research international 2013 (1), 269724 , 2013 2013 Citations: 105
Multiscale patterned transplantable stem cell patches for bone tissue regeneration J Kim, WG Bae, HW Choung, KT Lim, H Seonwoo, HE Jeong, KY Suh, ... Biomaterials 35 (33), 9058-9067 , 2014 2014 Citations: 103
Functionalized chitosan/spherical nanocellulose-based hydrogel with superior antibacterial efficiency for wound healing DK Patel, K Ganguly, J Hexiu, SD Dutta, TV Patil, KT Lim Carbohydrate Polymers 284, 119202 , 2022 2022 Citations: 98
Microfluidic nanomaterials: From synthesis to biomedical applications K Illath, S Kar, P Gupta, A Shinde, S Wankhar, FG Tseng, KT Lim, M Nagai, ... Biomaterials 280, 121247 , 2022 2022 Citations: 96
Mushroom-derived bioactive molecules as immunotherapeutic agents: A review DK Patel, SD Dutta, K Ganguly, SJ Cho, KT Lim Molecules 26 (5), 1359 , 2021 2021 Citations: 96
Electrically stimulated 3D bioprinting of gelatin-polypyrrole hydrogel with dynamic semi-IPN network induces osteogenesis via collective signaling and immunopolarization SD Dutta, K Ganguly, A Randhawa, TV Patil, DK Patel, KT Lim Biomaterials 294, 121999 , 2023 2023 Citations: 91
Bacterial cellulose nanofibrillar patch as a wound healing platform of tympanic membrane perforation J Kim, SW Kim, S Park, KT Lim, H Seonwoo, Y Kim, BH Hong, YH Choung, ... Advanced healthcare materials 2 (11), 1525-1531 , 2013 2013 Citations: 88