Dra. Esp. Maria Angelica Alvarez Paucar
@unmsm.edu.pe
Academic Department of Pediatric Dentistry
Universidad Nacional Mayor de San Marcos (UNMSM)
EDUCATION
Universidad Nacional Mayor de San Marcos
Pontificia Universidad Católica del Perú
RESEARCH INTERESTS
Pediatric Dentistry
Public health
Phytotherapeutics
Dental Research
Scopus Publications
Scopus Publications
Percy Romero-Tapia, Gabriela Sedano-Balbin, and Sonia Sacsaquispe-Contreras
Jaypee Brothers Medical Publishing
AIM
"Tocosh" is a potato that has undergone a process of hydraulic oxidation that enhances its antimicrobial properties so that this natural resource can be used in medical sciences. The aim of this study was to develop and evaluate the antibacterial and cytotoxic activity of a new experimental tooth based on Solanum tuberosum "Tocosh" on the cell lines 3T3 and DU145.
MATERIALS AND METHODS
To evaluate the cytotoxicity, cell cultures 3T3 and DU145 were used. Cell viability was determined by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in a medium containing 10% calf serum where the cells were preincubated at a concentration of 1 × 106 cells/mL in culture medium for 3 hours at 37°C and 6.5% CO2. Then, the absorbance was measured using a microplate reader where the formazan crystals were diluted with acidic and cold isopropanol, and quantified in an ELISA reader. To evaluate the antibacterial effect, the Kirby Bauer inhibition halos method was used on strains of S. aureus (ATCC 25923), S. mutans (ATCC 25175), and S. mitis (ATCC 49456).
RESULTS
Solanum tuberosum (tocosh) was not cytotoxic because it only had one CC50 at the concentration of 0.26927 mg/mL and 0.26845 mg/mL for the cell lines 3T3 and DU145, respectively. Tocosh toothpaste (TD) has an antibacterial effect against S. aureus and S. mutans.
CONCLUSION
The new ecological dentifrice was not cytotoxic since it did not alter cell viability because its CC50 was only 0.268 and 0.269 μg/mL for the 3T3 and DU145 cell lines, respectively; however, it presented an optimal antimicrobial activity against the oral strains evaluated.
CLINICAL SIGNIFICANCE
This research has great potential for clinical use because this new dentifrice has antimicrobial activity against different oral germs. How to cite this article: Mayta-Tovalino F, Sedano-Balbin G, Romero-Tapia P, et al. Development of New Experimental Dentifrice of Peruvian Solanum tuberosum (Tocosh) Fermented by Water Stress: Antibacterial and Cytotoxic Activity. J Contemp Dent Pract 2019;20(10):1206-1211.
G Mendoza-Azpur, María Álvarez-Paucar, Luis Gálvez-Calla, and Luz Carbajal
Jaypee Brothers Medical Publishing
Ab s t r Ac t Aim: The aim of the present study was to develop and validate the psychometric properties of the questionnaire (POCTOIR-30) for the evaluation of the perception of clinical training and future goals of residents of oral implantology. Materials and methods: The development of the questionnaire followed the process of five phases: (a) preparation of the instrument, (b) validation of the content, (c) validity of the construct, (d) evaluation of internal consistency and reliability, (e) evaluation of the level of knowledge, for which an instrument on implant training of residency programs was used as a reference. To evaluate the sensitivity and validity of the questionnaire, a pilot sample of 20 residents between 20 years and 50 years of a residency program in oral implantology was used. Results: The factor analysis of the data and the adjustment statistics process resulted in the quality care of 4 factors and 30 questions that were included in the present questionnaire (POCTOIR), which covers: logistics and infrastructure (five items), training in oral implantology (10 items); information on the postgraduate program (seven items) and accessibility and sustainability of care (ten items) and future goals after postgraduate studies (five items). Content validity was measured using the index that combines the ease of calculation and the evaluation of results at the statistical level (κ 0.833). The reliability of the instrument was evaluated to measure the degree of internal correlation (Cronbach’s α 0.738). Finally, the reliability was evaluated to measure the constancy of the responses repeatedly with the same subjects (intraclass correlation 0.74). Conclusion: This study showed that the POCTOIR-30 instrument has adequate levels of validity and reliability to be used in residents of areas related to oral implantology in new research. Clinical significance: The impact of the academic training of the residents in oral implantology significantly influences their performance at the time of performing the surgical and prosthetic protocols. Therefore, this research validates statistically the perception of academic training and future goals of postgraduate residents in order to quantify and apply improvement strategies during their years of study.
M. P. M. Rando-Meirelles, M. L. R. Sousa, G. C. Vale, V. A. Lages, P. P. Vásquez, P. Jimenez, R. S. Villena, and M. A. Alvarez-Paucar
Wiley
The aim of this pilot study was to address the reliability, internal validity and viability of oral health methods used in the South American Youth/Child cARdiovascular and Environmental study.
Frank Mayta-Tovalino, Yens Mendoza-Martiarena, Percy Romero-Tapia, María Álvarez-Paucar, Luis Gálvez-Calla, Juan Calderón-Sánchez, Rodolfo Bolaños-Cardenas, and Antonio Diaz-Sarabia
Hindawi Limited
Aim. To analyze the risk factors by logistic regression and perform the analysis of the survival rate of osseointegrated dental implants placed in public and private institutions. Methods. An analytic-multicentric study was carried out, where 1279 dental implants that were placed by specialists from January 2006 to October 2017 in public and private institutions (UPCH-SI, HCFAP, CMNAVAL, UPCH-SM, and UPSJB) were evaluated. The variables sex (X1), location (X2), hypertension (X3), antibiotic prophylaxis (X4), diabetes (X5), osteoporosis (X6), bisphosphonates (X7), history of periodontitis (X8), hypercholesterolemia (X9), bone quality (X10), bone quantity (X11), design (X12), smoker (X13), connection (X14), edentulism type (X15), staging (X16), 3D guided surgery (X17), load (X18), bone graft (X19), peri-implantitis (X20), mucositis (X21), and GBR (X22) were collected and analyzed by the Kaplan–Meier survival analysis. The logit analysis was performed among all the variables to choose the best statistical model that explains the true risk factors. The analysis was performed by multivariate logistic regression and the Kaplan–Meier test, at a level of statistical significance of p<0.05. Results. It was found that the failure rate of the 1279 implants evaluated was 17.98% corresponding to only 23 implants lost as they have good longevity over time. When establishing the best multivariate logistic regression model, it was found that the variables that remained stable in relation to their statistically significant value and more stable confidence intervals were age, osteoporosis, bisphosphonates, history of periodontitis, bone quality, bone graft, connection, number of implants, GBR (guided bone regeneration), and follow-up. Conclusions. Dental implants placed by specialists in public and private institutions had a failure rate similar to that in studies previously published in other countries.