ITM University Gwalior (MP)
-Ph. D in Biochemical Engineering (2 0 1 3 - 2 0 19) from the School of Biochemical Engineering, IIT (BHU), Varanasi, India.
-Master of Technology (M. Tech) in Biochemical Engineering (2010-2012) with first division (73.8 %) from the School of Biochemical Engineering, IIT (BHU),
-Bachelor of Technology (B. Tech) in Biotechnology Engineering (2006-2010) with first division (74 %) from IET Khandari Agra (Agra University), India
Drug Discovery, Biotechnology, General Biochemistry, Genetics and Molecular Biology, Bioengineering
These experiments involved the isolation of endophytes bacteria from an Adhathoda beddomei medicinal plant root (adosa). and obtaining new natural bioactive metabolites from natural resources is our goal. To solve these problems related to bioactive metabolite production, it is necessary to understand and explore the underlying mechanism by which endophytes produce bioactive metabolites. Important bioactive compounds were extracted from endophytes bacteria and their concentrations were increased using a variety of methods. (Media Optimization and Resonance Surface Technology-RSM). The antibacterial effectiveness against several pathogenic and nonpathogenic microorganisms was proven. With the use of a scanning electron microscope (SEM), endophytes were identified based on their exterior morphological traits. another challenging problem in this regard is that the efficiency of the endophytic bacterial to produce bioactive compounds is not as high as expected because in many previously wo
Abstract: Background: Adathoda beddomei (Adosa), a kind of softwood, evergreen, perennial shrub, was used as a source of endophyte bacteria for this study. Adhatoda beddomei shows a wide variety of chemicals, including anthocyanins, aminophylline, alkaloids, cardiac glycosides, isoprenaline, triterpenoids, resins, and proteolytic enzymes in latex, flavonoids, tannins, sterol, saponins, and Latex. The root, stem, and leaf parts of beddomei are most often used in indigenous medicine, but the root bark is also used to cure several conditions, including leprosy, fever, and bleeding. Objective: Some important bioactive metabolites were obtained from endophytes bacteria and have also been analyzed through various techniques (NMR, MASS, FTIR, HPLC, and UV- spectrophotometer) for the carried out of the functional group and empirical formulas of the bioactive secondary metabolites. Methods: In silico calculation, this bioactive metabolite has the potential to be antibacterial, and its primary
Neelam Tia, Moti Lal, and I. S. Gambhir Springer Science and Business Media LLC
Abstract Background Healthy aging perceives human longevity probably due to carrying the defensive genes. Forkhead box O (FOXO) transcription factors provide the most convincing example of a conserved genetic pathway at the point between aging and cancer. This pilot study was performed to examine the single nucleotide variants rs2253310 and rs4946936 of the Forkhead box O 3 (FOXO3 gene) in octogenarians and gastrointestinal tract (GIT) cancer patients in the north Indian population. Main body In silico mutational analysis of the FOXO3 gene in 25 participants. Two single nucleotide variants (SNVs) g.7556C>G (rs2253310) heterozygous and g.122284T>C (rs4946936) homozygous observed and reported previously. However, there is a common association of these SNVs in different ethnic groups. No significant differences in the genotype and allele frequencies for the study groups observed. Short conclusion This study observes two single nucleotide variants, g.7556C>G (rs2253310) and g.122284T>C (rs4946936), of the FOXO3 gene in the study groups which influence human longevity. Longevity-associated FOXO3 variants may be associated with GIT cancer in the north Indian population. As a result, looking for genes linked to longevity will lead to discovering new cancer targets. Further studies with a large population are necessary to elucidate the role of the FOXO3 gene in octogenarians.
Shiv Kumar Verma, Moti Lal, and Mira Debnath Innovare Academic Sciences Pvt Ltd
Objective:To study the antimicrobial activity of crud ethylacetate extract from endophytic fungus in Calotropis procera root.Methods:Endophytic fungus was screened for production of antimicrobial metablites.fermentation was carried out in 500 ml Erlenmeyer flask. Disc diffusion method was used to test antimicrobial activity of crude extract using chloramphenicol sulphate and Flucnazole as positive controleRessults: A total of fourteen Endophytic fungi were isolated (CPR1- CPR14). Among these fourteen isolates, CPR5 was found to show maximum antimicrobial activity, in compare to other isolates, against gram positive, gram negative bacteria and fungi. The antimicrobial activity was tested against Escherichia coli. Pseudomonas aeroginosa, Bacillus subtilis, Staphylococcus aureus, Ralstonia solanacearum, Xanthomonas oryzae, Penicillium chrysogenum, Candida albicans, Phoma exigua, Sclerotium rolfsii and Sclerotinia scleratiourum. Minimum inhibitory concentration (MIC) of crude extract against test microorganisms was determined. Fungus was identified as aspergillus sp. Prduction parameters (Temprature, pH, Carbon source, Nitrgen surce, Sdium Chlride concentration) were optimized.Conclusion: Crud extract produced by the isolated endophytic fungus could be an important source of broad spectrum antimicrobial metabolites. Keywords: Endophytic fungi, Antimicrobial metabolites, Process optimization, Calotropis procera, Inhibition zone.
Shiv Kr Verma, Moti Lal, and Mira Debnath Das Innovare Academic Sciences Pvt Ltd
Objective: In this research for bioactive secondary metabolites from endophytic fungal isolate CPR5, which was later identified and characterized as Aspergillus niger sp.Methods: Eight bioactive compounds (1-8) were isolated and purified using a series of chromatographic techniques and identified by applying 13C and 1H nuclear magnetic resonance and mass (ESI-MS spectrometry).Results: All the compounds were tested for antimicrobial activity against a wide range of human and plant pathogenic bacteria as well as fungi. These microorganisms were, Xanthomonas oryzae, Escherichia coli, Streptococcus pneumonia, Bacillus subtilis, Staphylococcus hyicus, Bacillus sphaericus, Staphylococcus aureus, and Pseudomonas aeruginosa, Candida albicans, Sclerotium rolfsii, Sclerotinia, scleratiourum, and Phoma exigua minimum inhibitory concentration value have also been determine for these microorganism.Conclusion: In this study, a total 10 endophytic fungi were isolated from the Calotropis procera, show a great antibacterial activity against 24 human pathogenic bacteria.
Ramesh Aggarwal, Atul Gawande, Moti Lal, and Kalachand Jana Medknow
Chronic myeloid leukemia may commonly present with atypical manifestations such as hearing loss or chloromas where the underlying diagnosis is revealed only on subsequent blood investigations. This case presented in the medicine emergency with one of the unusual complications of CML and so the diagnosis of CML was not suspected on the first instance. Prompt and comprehensive management of the patient not only saved him but also reiterated the fact that a rare presentation like priapism can sometimes forewarn of a more critical clinical condition.
1. Lal Moti, Tia Neelam, Verma Shiv Kr, Dutta Debashis, Das Mira Debnath, Characterization of Bioactive Metabolites from Endophytic Bacterial Isolate, Research
Journal of Biotechnology. Vol. 12 (1) January (2017). (Impact Factor: 0.8)
2. Tia, Neelam, Moti Lal, Chandra Shekhar Azad, Pritee Chaudhary, Manish Singh, and Indrajeet Singh Gambhir. "Serum Pentosidine Level in Healthy Ageing and
Its Related SN Comprehensive Clinical Medicine 2, no. 11 (2020): 2253-2259.
3. Neelam Tia, Moti Lal and I. S. Gambhir. rs2253310 and rs4946936 common variants of FOXO3 gene in octogenarians and cancer: a pilot study in north India.
Egyptian Journal of Medical Human Genetics. (2021) 22:47.
4. Lal Moti, Mira Debnath (Das). Recent Advance in an Antibacterial and Antifungal Natural Product. Int. J. Pharm. Sci. Rev. Res., 48(2), 2018 (Impact factor: 2.562)
5 Lal Moti, Neelam Tia, Debashis Dutta, Mira Debnath (Das). Bioactive Metabolites obtained from Endophytes Bacterial Isolate. International Journal of Innovative Research in Science, Engineering and Technology. Vol. 5, Issue 7, July 2016 (Impact factor: 7.08)
6Lal Moti, Neelam, Shiv Kr. Verma, Mira Debnath (Das). Studies of Bioactive Metabolites from Endophytic Bacterial Isolate. International Journal of Scientific & Engineering Research, Volume 7, Issue 6, June-2016. (Impact factor: 4.2)
7. Lal Moti, Shiv Kr. Verma, Vipin, Mira Debnath (Das). Thermo stability study of crude
Amylase from bacillus isolate. Int J Appl Sci
I have filed 03 patents on the FORMULATION OF NOVEL ANTIMICROBIAL AGENTS AND THERAPEUTIC DRUG.