Dr. Moti Lal

@itmuniversity.ac.in

Assistant Professor
ITM University Gwalior (MP)

Dr. Moti Lal


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https://researchid.co/motilal.bt

EDUCATION

-Ph. D in Biochemical Engineering (2 0 1 3 - 2 0 19) from the School of Biochemical Engineering, IIT (BHU), Varanasi, India.
-Master of Technology (M. Tech) in Biochemical Engineering (2010-2012) with first division (73.8 %) from the School of Biochemical Engineering, IIT (BHU),
Varanasi, India
-Bachelor of Technology (B. Tech) in Biotechnology Engineering (2006-2010) with first division (74 %) from IET Khandari Agra (Agra University), India

RESEARCH, TEACHING, or OTHER INTERESTS

Drug Discovery, Biotechnology, General Biochemistry, Genetics and Molecular Biology, Bioengineering

FUTURE PROJECTS

Studies and Enhancement of Natural Bioactive compounds content through Media Optimization

These experiments involved the isolation of endophytes bacteria from an Adhathoda beddomei medicinal plant root (adosa). and obtaining new natural bioactive metabolites from natural resources is our goal. To solve these problems related to bioactive metabolite production, it is necessary to understand and explore the underlying mechanism by which endophytes produce bioactive metabolites. Important bioactive compounds were extracted from endophytes bacteria and their concentrations were increased using a variety of methods. (Media Optimization and Resonance Surface Technology-RSM). The antibacterial effectiveness against several pathogenic and nonpathogenic microorganisms was proven. With the use of a scanning electron microscope (SEM), endophytes were identified based on their exterior morphological traits. another challenging problem in this regard is that the efficiency of the endophytic bacterial to produce bioactive compounds is not as high as expected because in many previously wo


Applications Invited

Recent developments and future prospects of natural Antimicrobial Bioactive Metabolites obtain from Endophytic Bacterial Isolate

Abstract: Background: Adathoda beddomei (Adosa), a kind of softwood, evergreen, perennial shrub, was used as a source of endophyte bacteria for this study. Adhatoda beddomei shows a wide variety of chemicals, including anthocyanins, aminophylline, alkaloids, cardiac glycosides, isoprenaline, triterpenoids, resins, and proteolytic enzymes in latex, flavonoids, tannins, sterol, saponins, and Latex. The root, stem, and leaf parts of beddomei are most often used in indigenous medicine, but the root bark is also used to cure several conditions, including leprosy, fever, and bleeding. Objective: Some important bioactive metabolites were obtained from endophytes bacteria and have also been analyzed through various techniques (NMR, MASS, FTIR, HPLC, and UV- spectrophotometer) for the carried out of the functional group and empirical formulas of the bioactive secondary metabolites. Methods: In silico calculation, this bioactive metabolite has the potential to be antibacterial, and its primary


Applications Invited
15

Scopus Publications

Scopus Publications

  • Industrial Applications of Microbial Secondary Metabolites: Diversity, Classification, and Sources
    Moti Lal, Neelam
    Industrial Applications of Microbial Secondary Metabolites A Modern Approach, 2026
  • Role of environmental factors on the development of PKDL
    Moti Lal, Neelam, Himanshu Gaur
    Visceral Leishmaniasis and Post Kala Azar Dermal Leishmaniasis Pathogenesis Treatment and Disease Control, 2025
  • Computational Network Pharmacology, Molecular Docking, and Molecular Dynamics to Decipher Natural Compounds of Alchornea laxiflora for Liver Cancer Chemotherapy
    Nem Kumar Jain, Balakumar Chandrasekaran, Nasha’t Khazaleh, Hemant Kumar Jain, Moti Lal, et al.
    Pharmaceuticals, 2025
    Background: Alchornea laxiflora (Benth.) Pax & K. Hoffm. (A. laxiflora) is utilized as a traditional herb for treating several diseases. Objective: Our study aims to identify the active phytochemical candidates from A. laxiflora and analyses to predict their anticancer activity mechanism by employing network pharmacology, molecular docking, and molecular dynamics (MD). Methods: The phytoconstituents of A. laxiflora were retrieved from the literature, and phytoconstituent-related targets implicated in hepatocellular carcinoma (HCC) were collected from respective databases. Computational methods were employed to recognize essential compounds, hub gene targets, and signaling pathways. Results: Our study has identified 12 potentially bioactive compounds, 150 potential anti-HCC targets, and 15 hub gene targets for A. laxiflora. Molecular docking results recognized the better binding energy values of below −5.6 kcal/mol. Further, MD simulations of the three of the top-scoring protein–ligand complexes (MAPK—3-acetylursolic acid, AKT1—quercetin, and AKT1—3-acetylursolic acid) allowed us to validate the docking results, evaluate the stability of the complexes, and associated conformational changes. Conclusions: Our research claims that phytoconstituents of A. laxiflora are crucial for treating liver cancer, and the recognized protein targets can serve as biomarkers, respectively.
  • Investigations into the Biochemical Properties of Penicillin G Amidase in Collagen Hydrogel and Collagen Hydrogel + Gelatin Combination Bio-composite Beads as Carriers
    Sudeshna Nag, Neelam Tia, Santosh Kumar, Varsha Chauhan, Moti Lal
    Current Enzyme Inhibition, 2025
    Introduction: Penicillin G amidase is an industrially significant enzyme widely employed in the production of semi-synthetic β-lactam antibiotics through the hydrolysis of Penicillin G to 6- aminopenicillanic acid. Owing to its commercial importance, extensive research has focused on improving the operational stability, reusability, and catalytic efficiency of PGA through various immobilization strategies. Methods: Optimization of multiple parameters for free and immobilized Penicillin G Acylase (PGA) is critical for improving the enzyme';s catalytic effectiveness, stability, and reusability in industrial and medicinal applications. This procedure entails methodically altering and analyzing variables such as substrate concentration, mechanical stability, cycle number, and storage conditions, and their effects on operational stability, pH, and temperature. PGA was optimized by entrapment on collagen hydrogel beads, resulting in collagen hydrogel + gelatin hybrid gel beads. Result: Immobilized PGA in Collagen Hydrogel + gelatin hybrid beads showed superior thermal stability, reusability, and storage stability as compared to gelatin-immobilized PGA. The entrapment of PGA onto Collagen Hydrogel + gelatin hybrid beads revealed several advantages and could be used in the production of 6-aminopenicillanic acid (6APA). Discussion: The study investigated the biochemical behavior of Penicillin G amidase (PGA) immobilized on collagen hydrogel and a collagen–gelatin bio-composite. Relative analysis focused on enzyme activity, stability, and mechanical strength, revealing insights into their appropriateness as immobilization matrices for enhanced PGA performance in industrial biocatalysis applications. Conclusion: Hydrogel + gelatin hybrid beads are more beneficial in industrial applications due to their greater stability and usability. PGA entrapment onto Hydrogel + gelatin hybrid beads has shown numerous advantages and may be useful in the manufacture of 6APA (6-aminopenicillanic acid).
  • Biological Potential and Different Pharmaceutical Applications of Bioactive Secondary Metabolites Obtained from Endophytic Fungus
    Moti Lal, Swati Rathore, Santosh Kumar, Rukhsar Khan, Akshay Singh Sengar, et al.
    Journal of Natural Remedies, 2024
    An analysis of the quantity and origins of bioactive agents can show the character of that natural products play in the finding of new therapeutic agents. At least 200,000 naturally occurring metabolites have different bioactive characteristics. Natural product discovery includes isolating, structurally elucidating, and establishing the bio-synthetic pathway of secondary metabolites. Scientists are very interested in this topic because of the structural diversity, complexity, and diverse bioactivities of isolated molecules. Secondary metabolites from marine-derived fungi have also been established to be quite important. The finding of novel species of endophytic fungi and the search for novel anti-fungi substances should continue.
  • Studies and Enhancement of Natural Bioactive Compounds Content through Media Optimization
    Moti Lal, Neelam, Santosh Kumar, Nem Kumar Jain, Akshay Singh Sengar, et al.
    Journal of Natural Remedies, 2024
    In these studies, endophyte bacteria were isolated from a medicinal plant root of Adhathoda beddomei (adosa). The antimicrobial activity was demonstrated against different pathogenic and nonpathogenic bacteria. Identification of endophytes was done based on external morphological characteristics with the help of a Scanning Electron Microscope (SEM). another challenging problem in this regard is that the efficiency of the endophytic bacteria to produce bioactive compounds is not as high as expected because, in many previous works in literature, it has been reported that yields are generally meagre. Through this study, we have tried to enhance the production of bioactive metabolites using media optimization and Resonance Surface Methodology (RSM). These are generally from µg/l to less than mg/l and are therefore not yet appropriate for fermentative production on an industrial level.
  • Recent Developments and Future Prospects of Natural Antimicrobial Bioactive Metabolites Obtained from Endophytic Bacterial Isolate
    Moti Lal, Neelam
    Current Bioactive Compounds, 2024
    Background: Adathoda beddomei (Adosa), a kind of softwood, evergreen, perennial shrub, has been used as a source of endophyte bacteria. Adhatoda beddomei has a wide variety of chemicals, including anthocyanins, aminophylline, alkaloids, cardiac glycosides, isoprenaline, triterpenoids, resins, flavonoids, tannins, sterol, saponins, etc. The root, stem, and leaf parts of Adathoda beddomei are most often used in indigenous medicine. Moreover, the root bark is also used to cure several conditions, including leprosy, fever, and bleeding. Objective: Some important bioactive metabolites were obtained from endophytes bacteria and analyzed through various techniques (NMR, MASS, FTIR, HPLC, and UV- spectrophotometer) for their bioactive secondary metabolites. Methods: In silico calculation was performed to reveal bioactive metabolites with the potential to be antibacterial, and their primary mode of action may include dissolving bacterial and fungal cell walls. Results: The antimicrobial activity of Adathoda beddomei was demonstrated against different pathogenic and non-pathogenic bacteria. Identification of endophytes was done based on external morphological characteristics with the help of a scanning electron microscope (SEM). Conclusion: Natural compounds derived from endophyte bacteria with a very low molecular mass can be used to discover new and important structures for different pharmaceuticals and agrochemicals.
  • Bacteriological profile of blood culture isolation and their antibiotic susceptibility pattern in BIMR Hospital, Gwalior, India
    Santosh Kumar, Pranshu Sharma, Anju Goyal, Sujeet Mrityunjay, Nem Kumar Jain, et al.
    International Journal of Biotechnology, 2023
  • rs2253310 and rs4946936 common variants of FOXO3 gene in octogenarians and cancer: a pilot study in north India
    Neelam Tia, Moti Lal, I. S. Gambhir
    Egyptian Journal of Medical Human Genetics, 2021
    Background Healthy aging perceives human longevity probably due to carrying the defensive genes. Forkhead box O (FOXO) transcription factors provide the most convincing example of a conserved genetic pathway at the point between aging and cancer. This pilot study was performed to examine the single nucleotide variants rs2253310 and rs4946936 of the Forkhead box O 3 (FOXO3 gene) in octogenarians and gastrointestinal tract (GIT) cancer patients in the north Indian population. Main body In silico mutational analysis of the FOXO3 gene in 25 participants. Two single nucleotide variants (SNVs) g.7556C>G (rs2253310) heterozygous and g.122284T>C (rs4946936) homozygous observed and reported previously. However, there is a common association of these SNVs in different ethnic groups. No significant differences in the genotype and allele frequencies for the study groups observed. Short conclusion This study observes two single nucleotide variants, g.7556C>G (rs2253310) and g.122284T>C (rs4946936), of the FOXO3 gene in the study groups which influence human longevity. Longevity-associated FOXO3 variants may be associated with GIT cancer in the north Indian population. As a result, looking for genes linked to longevity will lead to discovering new cancer targets. Further studies with a large population are necessary to elucidate the role of the FOXO3 gene in octogenarians.
  • Serum Pentosidine Level in Healthy Ageing and Its Association with Age-Related Disease
    Neelam Tia, Moti Lal, Chandra Shekhar Azad, Pritee Chaudhary, Manish Singh, et al.
    SN Comprehensive Clinical Medicine, 2020
  • Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from calotropis Procera root
    Shiv Kumar Verma, Moti Lal, Mira Debnath
    Asian Journal of Pharmaceutical and Clinical Research, 2017
  • Structural elucidation of bioactive secondary metabolites from endophytic fungus
    Shiv Kr Verma, Moti Lal, Mira Debnath Das
    Asian Journal of Pharmaceutical and Clinical Research, 2017
  • Characterisation of bioactive metabolites from endophytic bacterial isolate
    Research Journal of Biotechnology, 2017
  • Priapism: A chronic myeloid leukemia harbinger in exigency
    Ramesh Aggarwal, Atul Gawande, Moti Lal, Kalachand Jana
    Annals of Tropical Medicine and Public Health, 2013
  • Phytotonic effect of carbendazim on greengram (Phaseolus radiatus) and control of cercospora leaf-spot (Cercospora canescens)
    Indian Journal of Agricultural Sciences, 2003

Publications

1. Lal Moti, Tia Neelam, Verma Shiv Kr, Dutta Debashis, Das Mira Debnath, Characterization of Bioactive Metabolites from Endophytic Bacterial Isolate, Research
Journal of Biotechnology. Vol. 12 (1) January (2017). (Impact Factor: 0.8)
2. Tia, Neelam, Moti Lal, Chandra Shekhar Azad, Pritee Chaudhary, Manish Singh, and Indrajeet Singh Gambhir. "Serum Pentosidine Level in Healthy Ageing and
Its Related SN Comprehensive Clinical Medicine 2, no. 11 (2020): 2253-2259.
3. Neelam Tia, Moti Lal and I. S. Gambhir. rs2253310 and rs4946936 common variants of FOXO3 gene in octogenarians and cancer: a pilot study in north India.
Egyptian Journal of Medical Human Genetics. (2021) 22:47.
4. Lal Moti, Mira Debnath (Das). Recent Advance in an Antibacterial and Antifungal Natural Product. Int. J. Pharm. Sci. Rev. Res., 48(2), 2018 (Impact factor: 2.562)
5 Lal Moti, Neelam Tia, Debashis Dutta, Mira Debnath (Das). Bioactive Metabolites obtained from Endophytes Bacterial Isolate. International Journal of Innovative Research in Science, Engineering and Technology. Vol. 5, Issue 7, July 2016 (Impact factor: 7.08)
6Lal Moti, Neelam, Shiv Kr. Verma, Mira Debnath (Das). Studies of Bioactive Metabolites from Endophytic Bacterial Isolate. International Journal of Scientific & Engineering Research, Volume 7, Issue 6, June-2016. (Impact factor: 4.2)
7. Lal Moti, Shiv Kr. Verma, Vipin, Mira Debnath (Das). Thermo stability study of crude
Amylase from bacillus isolate. Int J Appl Sci

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

I have filed 03 patents on the FORMULATION OF NOVEL ANTIMICROBIAL AGENTS AND THERAPEUTIC DRUG.