PHYTOCHEMICAL PROFILING, ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF TURMERIC-GINGER COMBINATION EXTRACT AGAINST CANCER CELL LINE S Haryanti, Y Widiyastuti, H Widodo, R Mujahid, D Safrina, et al. Journal of Animal and Plant Sciences, 2025 Turmeric is recognized for various health benefits, commonly used in nutraceuticals and complementary medicine to alleviate pain and inflammation. It boosts the immune system and is also used for cancer treatment. The challenge in developing of turmeric formulation is the low bioavailability of curcumin as main compound. Literature reviews indicate that ginger is capable of improving blood circulation and enhancing the bioavailability of some compounds, including curcumin. This study aimed to examine the phytochemical profile, cytotoxic activity and antioxidant potential of the turmeric-ginger combination. The extraction was carried out by maceration 3x24 hours using 70% ethanol, evaporated, and dried in the oven. The thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used to obtain phytochemical profiles. Cytotoxic effect was carried out by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). Antioxidant assay was evaluated through H2O2-induced intracellular reactive oxygen species (ROS) quantification using flow cytometer. The HPLC profile revealed that curcumin levels in the turmeric-ginger combination ranged from 6-8%, with gingerol 4-11%. In T47D, HeLa, and WiDr cells, turmeric alone exhibited strong cytotoxicity, while ginger was less effective, and the combination actually reduced cytotoxic effect. However, in HepG2 cells, both turmeric and ginger alone exhibited weak cytotoxicity, surprisingly turmeric-ginger combination 50:50 showed synergistic effect. In Vero cells, ginger 25 and 50 µg/mL demonstrated a more significant capacity to decrease intracellular ROS levels induced by H2O2 when compared to turmeric. The combinations of turmeric ginger 70:30, 60:40, and 50:50 revealed a synergistic effect in reducing ROS levels. This research provides scientific evidence with a synergistic approach supporting the combination of turmeric-ginger to alleviate cancer growth and inhibit ROS generation. Key words: turmeric, ginger, formulation, ROS generation, cytotoxic
Ethnomedical uses of Cananga odorata (Lam.) Hook. f. and Thomson in indigenous traditional medicine among Indonesia ethnic groups Nuning Rahmawati, Ika Yanti M Sholikhah, Rohmat Mujahid, Yuli Widiyastuti, Agung Endro Nugroho, et al. Journal of Applied Pharmaceutical Science, 2024 Cananga odorata, commonly known as ylang-ylang, is an evergreen perennial tropical tree belonging to the Annonaceae family, which is traditionally and scientifically reported to have various pharmacological activities. This indigenous medicine study was carried out to document the traditional usage of C. odorata for medicinal reasons by Indonesian ethnic groups. This is one of numerous initiatives aimed at preserving indigenous knowledge and medical practices maintained by the community. Data were acquired during 2015 and 2017 through in-depth interviews with selected traditional healers from each ethnic group throughout 34 Indonesian provinces, who were chosen using a purposive sampling approach based on inclusion criteria. The information gathered includes the demographic data of healers, medicinal plants used, potions, and their applications for health purposes. Following the completion of the interview with the informant, specimens of medicinal plants were collected. Data quantification was accomplished by examining the value of plant parts and doing a simple ranking analysis for multihealth purposes. This study revealed 42 traditional applications of C. odorata by 36 traditional healers from 28 ethnic groups in 16 provinces to overcome 18 diseases. Cananga odorata was primarily obtained from the woodland instead of the house garden and other environments. Skin disease was identified as the most prevalent indication of C. odorata, in agreement with the most commonly employed administration method, the external route. Leaves became the foremost prominent plant part utilized by healers. This study emphasized the significant role of traditional healers in healing various illnesses with C. odorata and the variations in C. odorata ethnomedical usage as a traditional remedy in Indonesia. Nonetheless, cultivating initiatives should be started as more than 60% of identified C. odorata has yet to be grown. The study’s findings are likely to benefit various stakeholders, serve as baseline data for further studies on the efficacy and safety of C. odorata, and contribute to efforts for developing health products that employ C. odorata.
Medicinal plants utilized for fitness disorders treatment by ethnic groups in Papua and West Papua Province, Indonesia Dian Susanti, Nuning Rahmawati, Ika Yanti M. Sholikhah, Rohmat Mujahid, Dyah Subositi, et al. Journal of Applied Pharmaceutical Science, 2023 Papua is one of Indonesia’s islands with a large biological wealth, including medicinal plants. This study aimed to identify the utilization of medicinal plants by selected traditional healers who met inclusion criteria to treat fitness disorders in ethnic groups of Papua and West Papua. Data was collected through interviews, observation, and sample collection. Data quantification was done by analyzing the use value parameters, simple preference ranking exercise, multi-health purpose species rank, and the plant parts value. This study revealed 43 concoction information details and identified the use of 24 plant species distributed in 19 families among 19 healers in 10 ethnic groups in Papua and West Papua. Cymbopogon citratus (DC.) Stapf (12.12%) and leaves (57.35%) were determined as the most prominent species and plant parts used. Morinda citrifolia was identified as the species with the biggest number of other treatable diseases, with a total score of 85. The most used plant families were Myrtaceae and Poaceae, each with a percentage of 12.12%. The study showed the critical role of medicinal plants and traditional healers in community health. However, conservation efforts must be initiated immediately since almost 40% of traditional healers still harvest the available plants and make no cultivation efforts.
Review on ginger (Zingiber officinale Roscoe): phytochemical composition, biological activities and authentication analysis A.A. Styawan, R.A. Susidarti, Purwanto, A. Windarsih, N. Rahmawati, et al. Food Research, 2022 Zingiber officinale Roscoe, known as ginger has been widely used as a spice in food application and as a herbal component in traditional medicine. Its rhizome is known to have bioactive compounds such as phenolic compounds, flavonoid compounds, and essential oils which are responsible for pharmacological activities. Gingerol is the major phenolic compound in the ginger rhizome which consist of gingerol, shogaol, paradol, zingerol, gingerones, and gingerdiones. Other compounds such as polysaccharides, amino acids, organic acids, and minerals are also present. Ginger provides health advantages for humans because of its biological activities such as antioxidant, antiinflammation, antibacterial, antiviral, antifungal, antihyperlipidemic, antiobesity, and hepatoprotective activities. Products developed from ginger rhizomes were used in foods, beverages, and herbal medicine. Due to its functional values and its wide application, it is very important to ensure its authenticity. Authentication is important for quality control because it is related to the safety, efficacy, and quality of the products. The high-performance liquid chromatography (HPLC), DNA-based method, and vibrational spectroscopy combined with chemometrics of multivariate analysis have been successfully used for ginger authentication. This review highlighted the phytochemical compositions, biological activities, and authentication analysis of ginger rhizome. Based on its biological activities, ginger is a good source of pharmaceutical and nutraceutical products.
Ficus septica, an ecosystem keystone species induced ROS-mediated cytotoxicity in HepG2 hepatocarcinoma cells S Haryanti, N Rahmawati, I Y M Sholikhah, Y Widiyastuti Iop Conference Series Earth and Environmental Science, 2021 Ficus septica grows all around Indonesia, as one of the key species in various ecosystem types. This plant is a food resource and habitat for some animals. This study aimed to examine cytotoxic activity of F. septica extract in HepG2 cells. The leaves powder was macerated using ethanol 96%. Cytotoxic activity was evaluated by MTT assay. The determination of cell cycle profile and reactive oxygen species (ROS) were done by flow cytometry. The extract inhibited the growth of HepG2 and Vero cells with an IC50 of 50.9 and 286.2 µg/mL, while doxorubicin 0.8 and 12.8 µg/mL. The selectivity index of the extract and doxorubicin was 5.6 and 16 respectively. The extract triggered cell cycle arrest in HepG2 at the G0/G1 phase, whereas doxorubicin in the S phase. The extract and doxorubicin significantly increased intracellular ROS in HepG2, but not in normal Vero cells. In conclusion, our findings suggest that F. septica induced cytotoxicity in HepG2 cells is mediated by excessive ROS generation leading to oxidative stress.
Medicinal plant utilization for hypercholesterolemia by traditional healers in Java island N Rahmawati, F I Mustofa, S Haryanti, R Mujahid Iop Conference Series Earth and Environmental Science, 2021 This ethnomedicinal study aimed to reveal the medicinal plant utilization as traditional medicine for preventing as well as treating hypercholesterolemia by traditional healers in Java Island including Banten, Yogyakarta, DKI Jakarta, West Java, East Java and Central Java Province of Indonesia. Data was collected based on purposive random sampling among five selected traditional healers in each ethnic in 2015. The results exhibited as of 38 medicinal plant species distributed in 24 families showed to have a pharmacological effect on treating hypercholesterolemia whereas Guazuma ulmifolia Lam. and Zingiberaceae were identified as the most prominent medicinal plant and family used by traditional healers. The most frequent plant part used was leaves (34.5%) followed by rhizomes (17.3%), fruits (12.7%), and others. Most medicinal plants were gained from house yard (37.6%) with planting efforts as of 45.9%. The most prescribed method by traditional healers was by drinking the formula (97.2%) as many as two times a day (59.63%). The study clearly demonstrated whether traditional healers had a pivotal role in overcoming ailments and disease especially hypercholesterolemia in Java Island.
PHYTOCHEMICAL PROFILING, ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF TURMERIC-GINGER COMBINATION EXTRACT AGAINST CANCER CELL LINE. S Haryanti, Y Widiyastuti, H Widodo, R Mujahid, DSD Subositi, ... JAPS: Journal of Animal & Plant Sciences 35 (2) , 2025 2025 Citations: 3
Ethnomedical uses of Cananga odorata (Lam.) Hook. f. and Thomson in indigenous traditional medicine among Indonesia ethnic groups N Rahmawati, IYM Sholikhah, R Mujahid, Y Widiyastuti, AE Nugroho, ... Journal of Applied Pharmaceutical Science 14 (9), 128-137 , 2024 2024 Citations: 7
Traditional uses of ginger (Zingiber officinale Roscoe) based on ethno-medicine study in 254 Indonesia ethnic groups N Rahmawati, IYM Sholikhah, D Subositi, FI Mustofa, S Haryanti, ... Indian Journal of Traditional Knowledge (IJTK) 23 (5), 464-472 , 2024 2024 Citations: 4
A novel mathematical model for quality optimization of textile products during shipment SH Putri, F Abdullah, N Rahmawati, CC Dayani, A Kusumadewi JURNAL INTECH TEKNIK INDUSTRI UNIVERSITAS SERANG RAYA Учредители … , 2024 2024 Citations: 6
Medicinal plants utilized for fitness disorders treatment by ethnic groups in Papua and West Papua Province, Indonesia D Susanti, N Rahmawati, IYM Sholikhah, R Mujahid, D Subositi, ... Journal of Applied Pharmaceutical Science 13 (9), 149-160 , 2023 2023 Citations: 8
Application of Raman spectroscopy and chemometrics for quality controls of fats and oils: a review A Windarsih, L Arsanti Lestari, Y Erwanto, A Rosiana Putri, Irnawati, ... Food Reviews International 39 (7), 3906-3925 , 2023 2023 Citations: 30
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Ethnopharmacology Study of Medicinal Plants Utilized for Hypercholesterolemia Treatment on Borneo Island of Indonesia N Rahmawati, F Indrian Mustofa, S Haryanti, D Subositi, H Widodo, ... Jurnal Tumbuhan Obat Indonesia 15 (1), 1-15 , 2022 2022 Citations: 10
Genetic diversity and chemicals profile of ginger (Zingiber officinale Roscoe) in Indonesia D Subositi, H Widodo, RM Mujahid, N Rahmawati, FI Mustofa, M Indrian, ... Int J Adv Sci Eng Inform Technol 12 (3), 929-936 , 2022 2022 Citations: 5
Aktivitas Anti-Dermatitis dan Gambaran Toksisitas Akut Ramuan Sembung (Blumea balsamifera DC.), Jahe (Zingiber officinale Roscoe), Rumput Teki (Cyperus rotundus L.), dan Cabe … G Ratnawati, IYM Sholikhah, N Rahmawati, A Wuryani Jurnal Tumbuhan Obat Indonesia 14 (2), 156-163 , 2021 2021 Citations: 1
UJI AKTIVITAS ANTI-DERMATITIS RAMUAN SEMBUNG, JAHE, RUMPUT TEKI, DAN CABE JAWA PADA TIKUS MODEL INDUKSI OVALBUMIN DAN UJI TOKSISITAS AKUTNYA G Ratnawati, I Yanti, N Rahmawati, A Wuryani Jurnal Tumbuhan Obat Indonesia 14 (2) , 2021 2021 Citations: 1
Ethnomedicine of medicinal plants used by traditional healers to facilitate bone injury healing in West Kalimantan, Indonesia FI Mustofa, N Rahmawati, S Saryanto Jurnal Tumbuhan Obat Indonesia 14 (1), 36-54 , 2021 2021 Citations: 11
Medicinal plant utilization for hypercholesterolemia by traditional healers in Java island N Rahmawati, FI Mustofa, S Haryanti, R Mujahid IOP Conference Series: Earth and Environmental Science 637 (1), 012043 , 2021 2021 Citations: 4
Medicinal plants and practices of Rongkong traditional healers in South Sulawesi, Indonesia FI Mustofa, N Rahmawati Biodiversitas 21 (2), 642-651 , 2020 2020 Citations: 28
Curcuma zanthorrhiza Extracts Induce G2/M cell cycle arrest and apoptosis in 4T1 and MCF-7 human breast cancer cells S Haryanti, G Ratnawati, N Rahmawati 4th International Symposium on Health Research (ISHR 2019), 415-419 , 2020 2020 Citations: 4
Medicinal plants used by traditional healers for the treatment of various diseases in ondae sub-ethnic of Poso District in Indonesia N Rahmawati, Y Widiyastuti, R Purwanto, SS Lestari, IHA Sene, Y Bakari 4th International Symposium on Health Research (ISHR 2019), 460-468 , 2020 2020 Citations: 13
Diversity of medicinal plants utilized by To Manui ethnic of Central Sulawesi, Indonesia N Rahmawati, FI Mustofa, S Haryanti Biodiversitas 21 (1), 375-392 , 2020 2020 Citations: 31
Budidaya dan manfaat sirih untuk kesehatan Y Widiyastuti, N Rahmawati, R Mujahid Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan , 2020 2020 Citations: 7
TOKSISITAS SUBKRONIS KOMBINASI TEMULAWAK, KUNYIT DAN MENIRAN TERHADAP FUNGSI HEPAR DAN GINJAL TIKUS UJI N Rahmawati, G Ratnawati Badan Penelitian dan Pengembangan Kesehatan , 2019 2019
THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION … N Rahmawati, IYM Sholikhah Jurnal Tumbuhan Obat Indonesia 12 (1), 33-40 , 2019 2019 Citations: 1
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Gastroprotective effect of combination of hot water extracts of Licorice (Glycyrrhiza glabra), Pulasari Stem Bark (Alyxia reinwardtii), and Sembung Leaf (Blumea balsamifera … AE Nugroho, A Wijayanti, M Mutmainah, R Susilowati, N Rahmawati Journal of evidence-based complementary & alternative medicine 21 (4), NP77-NP84 , 2016 2016 Citations: 38
Studi etnofarmakologi tumbuhan obat yang digunakan oleh penyehat tradisional untuk mengatasi diare di Sulawesi Selatan FI Mustofa, N Rahmawati Jurnal Tumbuhan Obat Indonesia 11 (2), 17-32 , 2018 2018 Citations: 36
Diversity of medicinal plants utilized by To Manui ethnic of Central Sulawesi, Indonesia N Rahmawati, FI Mustofa, S Haryanti Biodiversitas 21 (1), 375-392 , 2020 2020 Citations: 31
Application of Raman spectroscopy and chemometrics for quality controls of fats and oils: a review A Windarsih, L Arsanti Lestari, Y Erwanto, A Rosiana Putri, Irnawati, ... Food Reviews International 39 (7), 3906-3925 , 2023 2023 Citations: 30
The aphrodisiac effect and toxicity of combination Piper retrofractum L, Centella asiatica, and Curcuma domestica infusion N Rahmawati, MS Bachri Health Science Journal of Indonesia 3 (1 Jun), 19-22 , 2012 2012 Citations: 30
Gastroprotective effects of combination of hot water extracts of turmeric (Curcuma domestica L.), cardamom pods (Ammomum compactum S.) and sembung leaf (Blumea balsamifera DC … R Susilowati, N Rahmawati, AE Nugroho Asian Pacific Journal of Tropical Biomedicine 4, S500-S504 , 2014 2014 Citations: 29
Medicinal plants and practices of Rongkong traditional healers in South Sulawesi, Indonesia FI Mustofa, N Rahmawati Biodiversitas 21 (2), 642-651 , 2020 2020 Citations: 28
Phytochemicals and antidiabetic activity of Eusideroxylon zwageri stem bark collected from East Kalimantan, Indonesia IW Kusuma, Rahmini, R Ramadhan, N Rahmawati, RA Suwasono, ... IOP conference series: Earth and environmental science 144 (1), 012030 , 2018 2018 Citations: 19
Medicinal plants used by traditional healers for the treatment of various diseases in ondae sub-ethnic of Poso District in Indonesia N Rahmawati, Y Widiyastuti, R Purwanto, SS Lestari, IHA Sene, Y Bakari 4th International Symposium on Health Research (ISHR 2019), 460-468 , 2020 2020 Citations: 13
Analysis of curcumin in ethanolic extract of Curcuma longa Linn. and Curcuma xanthorriza Roxb. using high performance liquid chromatography with UV-detection. A Nugroho, A Rohman, E Lukitaningsih, N Rakhmawati, S Sudjadi 2015 Citations: 13
Ethnomedicine of medicinal plants used by traditional healers to facilitate bone injury healing in West Kalimantan, Indonesia FI Mustofa, N Rahmawati, S Saryanto Jurnal Tumbuhan Obat Indonesia 14 (1), 36-54 , 2021 2021 Citations: 11
Ethnopharmacology Study of Medicinal Plants Utilized for Hypercholesterolemia Treatment on Borneo Island of Indonesia N Rahmawati, F Indrian Mustofa, S Haryanti, D Subositi, H Widodo, ... Jurnal Tumbuhan Obat Indonesia 15 (1), 1-15 , 2022 2022 Citations: 10
Cytotoxic and MMPs inhibitory activities of Sappan Wood (Caesalpinia sappan L.): various extracts on 4T1 breast cancer cell line S Haryanti, Y Widiyastuti, N Rahmawati Health Science Journal of Indonesia 9 (1), 51-56 , 2018 2018 Citations: 10
Medicinal plants utilized for fitness disorders treatment by ethnic groups in Papua and West Papua Province, Indonesia D Susanti, N Rahmawati, IYM Sholikhah, R Mujahid, D Subositi, ... Journal of Applied Pharmaceutical Science 13 (9), 149-160 , 2023 2023 Citations: 8
Mechanism of action vasodilation Annona muricata L. leaves extract mediated vascular smooth muscles S Ismail, N Hayati, N Rahmawati IOP Conference Series: Earth and Environmental Science 144 (1), 012006 , 2018 2018 Citations: 8
Ethnomedical uses of Cananga odorata (Lam.) Hook. f. and Thomson in indigenous traditional medicine among Indonesia ethnic groups N Rahmawati, IYM Sholikhah, R Mujahid, Y Widiyastuti, AE Nugroho, ... Journal of Applied Pharmaceutical Science 14 (9), 128-137 , 2024 2024 Citations: 7
Budidaya dan manfaat sirih untuk kesehatan Y Widiyastuti, N Rahmawati, R Mujahid Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan , 2020 2020 Citations: 7
A novel mathematical model for quality optimization of textile products during shipment SH Putri, F Abdullah, N Rahmawati, CC Dayani, A Kusumadewi JURNAL INTECH TEKNIK INDUSTRI UNIVERSITAS SERANG RAYA Учредители … , 2024 2024 Citations: 6
Genetic diversity and chemicals profile of ginger (Zingiber officinale Roscoe) in Indonesia D Subositi, H Widodo, RM Mujahid, N Rahmawati, FI Mustofa, M Indrian, ... Int J Adv Sci Eng Inform Technol 12 (3), 929-936 , 2022 2022 Citations: 5
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