Dr. Rajani Sharma
@amity.edu
Assistant Professor
Amity University Jharkhand
RESEARCH INTERESTS
Bioinformatics, Immunology, Biochemistry and microbiology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Prince Kumar, Rajani Sharma and K. Kumar
Springer Science and Business Media LLC
Chinmay Patade, Bhavna Sharma, Sneha Kumari, Rajani Sharma, and Shubha Rani Sharma
Elsevier
Bhavna Sharma, Rajani Sharma, Ram Prasad, and Shubha Rani Sharma
Elsevier
Rajani Sharma, Shubha Rani Sharma, and Ram Prasad
Elsevier
Rajani Sharma and Kunal Kumar
Wiley
Kunal Kumar, Rajani Sharma, Ashutosh Kumar, and Sushant Singh
Elsevier BV
Rajani Sharma, Kunal Kumar, and Kumari Tanvi
Informa UK Limited
FXR (Farnesoid X Receptor) is one of the nuclear receptors expressed in the liver performing a significant role in the maintenance of bile acid concentration. An imbalance of cholesterol and bile acid ratio due to any undefined reason could cause gallstone formation. Hence, this paper aims to screen phytochemicals that could maintain a requisite balance of cholesterol and bile acid by targeting FXR and thereby contributing to the dissolution of gallstone. Nineteen phytochemicals were selected and queried for Pa and Pi in the way2drug online server for hepatoprotective property, cholesterol synthesis and absorption inhibition property, and β-glucuronidase inhibiting activity. Cianidanol, neoandrographolide, cynarine, saponins, and tanins with satisfying stated properties were docked with the screened FXR (PDB ID- 1OSH) using HADDOCK server, followed by pharmacokinetics study utilizing SwissADME tool. Neoandrographolide fits best among the other selected literature-based phytochemicals with minor violation of 'Brenk's rule'. The violation was corrected with the removal of an alkene group in the provided ChemDraw space of SwissADME. This Dealkenylated compound was further docked with FXR. The promising response under the static condition of the Dealkenylated compound was analyzed for molecular dynamic simulation at physiological conditions for 100 ns. Dealkenylated Neoandrographolide (DN) exhibited hepatoprotective, cholesterol synthesis and absorption inhibition property, and β-glucuronidase inhibition activity with a superior binding score of -42.6+/-1.5 with FXR. The interaction of the FXR receptor and the DN showed exceptional stability at physiological conditions during MD simulation and fit for the ADME properties, therefore it could be a potent candidate to dissolve gallstones.Communicated by Ramaswamy H. Sarma.
Kunal Kumar and Rajani Sharma
Wiley
Vijay Kant Pandey, Rajani Sharma, Gopal Kumar Prajapati, Tapan Kumar Mohanta, and Awdhesh Kumar Mishra
Springer Science and Business Media LLC
Bhavna Sharma, Usha Lakra, Rajani Sharma, and Shubha Rani Sharma
Elsevier
Rajani Sharma and Shubha Rani Sharma
Springer International Publishing
Rajani Sharma, Gopal Kumar Prajapati, and Gargi Akhoury
Brill
Abstract COVID-19 is the current health challenge across the world. It originated in Wuhan, China, and has now spread to more than 180 countries. It is a zoonotic disease which spreads through droplets. The severity of disease is likely to end with the discovery of vaccines only. Researchers are repurposing drugs to fill the gap between COVID-19 and vaccine designing. Broad-spectrum antiviral drugs are preferred but they exhibit side effects. We have screened pentagalloylglucose present in Terminalia chebula which can prevent SARS-CoV-2 entry to the host cell. In this study, we have taken 8 active phytochemicals of Terminalia chebula which include gallic acid, chebulic acid, chebulanin, neochebulinic acid, ellagic acid, chebulagic acid, chebulinic acid, and pentagalloyglucose against spike proteins (S1 and S2), Replicase Polyprotein, 3C-like protease (3CL pro), Papain-like protease (PLpro), RNA dependent RNA polymerase (RdRp) of SARS-CoV-2. HADDOCK online server, Discovery Studio Visualizer and PyRx Vina tools were used to screen the potential component from T. chebula. It was analysed that pentagalloylglucose can be a better phytochemical against spike protein S1 similar to hemagglutinin of influenza virus. This phytochemical can be further used as a drug against SARS-CoV-2.
Rajani Sharma and Gargi Akhoury
Springer Science and Business Media LLC
Rajani Sharma, Shashwati Ghosh Sachan, and Shubha Rani Sharma
Springer Science and Business Media LLC
Rajani Sharma, Snehi Soy, Chandan Kumar, Shashwati Ghosh Sachan, and Shubha Rani Sharma
Springer Science and Business Media LLC
Rajani Sharma, Shashwati Ghosh Sachan, and Shubha Rani Sharma
Briefland
Background: Bacteria are commonly isolated from gallstones. Presence of bacteria in gallstones might play a role in their formation. Objectives: We aimed to study different properties of isolates from gallstones and their relationship with the mechanism of gallstone formation. Patients and Methods: This study included 526 subjects. Gallstones and bile samples of the patients were cultured. Isolates were identified by different biochemical tests and examined for their β-glucuronidase activity, slime production and Urease activity. Biostatistical analyses were done to determine the relationship between the biliary bacterial factors and the types of gallstones. Role of Slime and urease activity in mechanism of gallstone formation were tested in vitro. Results: Overall, 94.77% of the gallstones were found to be infected, of which 85% of the cholesterol stones, 97% of pigmented stones and 100% of mixed stones showed the presence of bacterial isolates (P < 0.0001). β-glucuronidase activity was associated with isolates from pigmented or mixed stones and completely absent in cholesterol gallstones (P = 0.018). Urease activity was basically associated with cholesterol gallstones (P < 0.0001) while slime (P < 0.0001) activity was not specific for any single type of stone formation yet isolates of bile had a significant association with slime activity. In vitro analysis demonstrated that bacteria with urease activity are involved in CaCO 3 precipitation and slime-producing isolates solidify the precipitated CaCO 3 . Conclusions: β-glucuronidase activity is mainly responsible for pigmented gallstone formation. Urease activity was predicted to be involved in nucleation and slime production during solidification of gallstones, as observed during in vitro studies. Hence, presence of these bacterial factors may enhance gallstone formation.