Dr. Rajani Sharma

@amity.edu

Assistant Professor
Amity University Jharkhand



                 

https://researchid.co/rajanibio

RESEARCH INTERESTS

Bioinformatics, Immunology, Biochemistry and microbiology

18

Scopus Publications

164

Scholar Citations

6

Scholar h-index

5

Scholar i10-index

Scopus Publications

  • A perspective on varied fungal virulence factors causing infection in host plants
    Prince Kumar, Rajani Sharma and K. Kumar
    Springer Science and Business Media LLC

  • Case study of gallbladder diseases and microbiome
    Chinmay Patade, Bhavna Sharma, Sneha Kumari, Rajani Sharma, and Shubha Rani Sharma
    Elsevier

  • COMPUTATIONAL BIOLOGY IN DRUG DISCOVERY AND REPURPOSING


  • Historical perspective of gallstones
    Bhavna Sharma, Rajani Sharma, Ram Prasad, and Shubha Rani Sharma
    Elsevier


  • Preface
    Rajani Sharma, Shubha Rani Sharma, and Ram Prasad
    Elsevier

  • Dysbiosis and its varied impacts
    Rajani Sharma and Kunal Kumar
    Wiley


  • Dealkenylation of neoandrographolide, a phytochemical from Andrographis paniculata stimulates FXR (Farnesoid X Receptor) and enhances gallstone dissolution
    Rajani Sharma, Kunal Kumar, and Kumari Tanvi
    Informa UK Limited
    FXR (Farnesoid X Receptor) is one of the nuclear receptors expressed in the liver performing a significant role in the maintenance of bile acid concentration. An imbalance of cholesterol and bile acid ratio due to any undefined reason could cause gallstone formation. Hence, this paper aims to screen phytochemicals that could maintain a requisite balance of cholesterol and bile acid by targeting FXR and thereby contributing to the dissolution of gallstone. Nineteen phytochemicals were selected and queried for Pa and Pi in the way2drug online server for hepatoprotective property, cholesterol synthesis and absorption inhibition property, and β-glucuronidase inhibiting activity. Cianidanol, neoandrographolide, cynarine, saponins, and tanins with satisfying stated properties were docked with the screened FXR (PDB ID- 1OSH) using HADDOCK server, followed by pharmacokinetics study utilizing SwissADME tool. Neoandrographolide fits best among the other selected literature-based phytochemicals with minor violation of 'Brenk's rule'. The violation was corrected with the removal of an alkene group in the provided ChemDraw space of SwissADME. This Dealkenylated compound was further docked with FXR. The promising response under the static condition of the Dealkenylated compound was analyzed for molecular dynamic simulation at physiological conditions for 100 ns. Dealkenylated Neoandrographolide (DN) exhibited hepatoprotective, cholesterol synthesis and absorption inhibition property, and β-glucuronidase inhibition activity with a superior binding score of -42.6+/-1.5 with FXR. The interaction of the FXR receptor and the DN showed exceptional stability at physiological conditions during MD simulation and fit for the ADME properties, therefore it could be a potent candidate to dissolve gallstones.Communicated by Ramaswamy H. Sarma.


  • N-glycosylation, a leading role in viral infection and immunity development
    Vijay Kant Pandey, Rajani Sharma, Gopal Kumar Prajapati, Tapan Kumar Mohanta, and Awdhesh Kumar Mishra
    Springer Science and Business Media LLC

  • A comprehensive review on nanopesticides and nanofertilizers—A boon for agriculture
    Bhavna Sharma, Usha Lakra, Rajani Sharma, and Shubha Rani Sharma
    Elsevier

  • Microbial Aspect in Wastewater Management: Biofilm
    Rajani Sharma and Shubha Rani Sharma
    Springer International Publishing

  • Pentagalloylglucose, a phytochemical from Terminalia chebula can efficiently prevent SARS-CoV-2 entry: In Silico study
    Rajani Sharma, Gopal Kumar Prajapati, and Gargi Akhoury
    Brill
    Abstract COVID-19 is the current health challenge across the world. It originated in Wuhan, China, and has now spread to more than 180 countries. It is a zoonotic disease which spreads through droplets. The severity of disease is likely to end with the discovery of vaccines only. Researchers are repurposing drugs to fill the gap between COVID-19 and vaccine designing. Broad-spectrum antiviral drugs are preferred but they exhibit side effects. We have screened pentagalloylglucose present in Terminalia chebula which can prevent SARS-CoV-2 entry to the host cell. In this study, we have taken 8 active phytochemicals of Terminalia chebula which include gallic acid, chebulic acid, chebulanin, neochebulinic acid, ellagic acid, chebulagic acid, chebulinic acid, and pentagalloyglucose against spike proteins (S1 and S2), Replicase Polyprotein, 3C-like protease (3CL pro), Papain-like protease (PLpro), RNA dependent RNA polymerase (RdRp) of SARS-CoV-2. HADDOCK online server, Discovery Studio Visualizer and PyRx Vina tools were used to screen the potential component from T. chebula. It was analysed that pentagalloylglucose can be a better phytochemical against spike protein S1 similar to hemagglutinin of influenza virus. This phytochemical can be further used as a drug against SARS-CoV-2.

  • 2019-nCoV: a worldwide concern and facts
    Rajani Sharma and Gargi Akhoury
    Springer Science and Business Media LLC

  • In vitro analysis of gallstone formation in the presence of bacteria
    Rajani Sharma, Shashwati Ghosh Sachan, and Shubha Rani Sharma
    Springer Science and Business Media LLC

  • Analysis of gallstone composition and structure in Jharkhand region
    Rajani Sharma, Snehi Soy, Chandan Kumar, Shashwati Ghosh Sachan, and Shubha Rani Sharma
    Springer Science and Business Media LLC

  • Urease positive and slime producing bacterial activity: Results in gallstone precipitation and solidification
    Rajani Sharma, Shashwati Ghosh Sachan, and Shubha Rani Sharma
    Briefland
    Background: Bacteria are commonly isolated from gallstones. Presence of bacteria in gallstones might play a role in their formation. Objectives: We aimed to study different properties of isolates from gallstones and their relationship with the mechanism of gallstone formation. Patients and Methods: This study included 526 subjects. Gallstones and bile samples of the patients were cultured. Isolates were identified by different biochemical tests and examined for their β-glucuronidase activity, slime production and Urease activity. Biostatistical analyses were done to determine the relationship between the biliary bacterial factors and the types of gallstones. Role of Slime and urease activity in mechanism of gallstone formation were tested in vitro. Results: Overall, 94.77% of the gallstones were found to be infected, of which 85% of the cholesterol stones, 97% of pigmented stones and 100% of mixed stones showed the presence of bacterial isolates (P < 0.0001). β-glucuronidase activity was associated with isolates from pigmented or mixed stones and completely absent in cholesterol gallstones (P = 0.018). Urease activity was basically associated with cholesterol gallstones (P < 0.0001) while slime (P < 0.0001) activity was not specific for any single type of stone formation yet isolates of bile had a significant association with slime activity. In vitro analysis demonstrated that bacteria with urease activity are involved in CaCO 3 precipitation and slime-producing isolates solidify the precipitated CaCO 3 . Conclusions: β-glucuronidase activity is mainly responsible for pigmented gallstone formation. Urease activity was predicted to be involved in nucleation and slime production during solidification of gallstones, as observed during in vitro studies. Hence, presence of these bacterial factors may enhance gallstone formation.

RECENT SCHOLAR PUBLICATIONS

  • A perspective on varied fungal virulence factors causing infection in host plants
    P Kumar, R Sharma, K Kumar
    Molecular Biology Reports 51 (1), 392 2024

  • Historical perspective of gallstones
    B Sharma, R Sharma, R Prasad, SR Sharma
    Gallstone Formation, Diagnosis, Treatment and Prevention, 1-7 2024

  • Dysbiosis and its Varied Impacts
    R Sharma, K Kumar
    The Gut Microbiota in Health and Disease, 41-54 2023

  • Dealkenylation of neoandrographolide, a phytochemical from Andrographis paniculata stimulates FXR (Farnesoid X Receptor) and enhances gallstone dissolution
    R Sharma, K Kumar, K Tanvi
    Journal of Biomolecular Structure and Dynamics 41 (8), 3339-3348 2023

  • Impact of oxalate on the physicochemical properties of potato starch: An insight closely associated with Indian cuisine
    K Kumar, R Sharma, A Kumar, S Singh
    International Journal of Gastronomy and Food Science 31, 100660 2023

  • Orchestration of the Plant Microbiome for Enhanced Agriculture
    SR Sharma, R Sharma
    Sustainable Agriculture Reviews 60: Microbial Processes in Agriculture, 23-46 2023

  • Role of Probiotics in Treatment of Gut‐Related Diseases
    K Kumar, R Sharma
    Prebiotics and Probiotics in Disease Regulation and Management, 1-26 2022

  • Novel Microbial Compounds as a Boon in Health Management
    SR Sharma, R Sharma, D Kar
    Biotechnological Advances for Microbiology, Molecular Biology, and 2022

  • N-glycosylation, a leading role in viral infection and immunity development.
    VK Pandey, R Sharma, GK Prajapati, TK Mohanta, AK Mishra
    Mol Biol Rep 2022

  • A comprehensive review on nanopesticides and nanofertilizers—A boon for agriculture
    B Sharma, U Lakra, R Sharma, SR Sharma
    Nano-enabled Agrochemicals in Agriculture 1, 1-18 2022

  • Microbial Aspect in Wastewater Management: Biofilm.
    R Sharma, SR Sharma
    Advanced Industrial Wastewater Treatment and Reclamation of Water., 71-86 2021

  • Pentagalloylglucose, a phytochemical from Terminalia chebula can efficiently prevent SARS-CoV-2 entry: In Silico study
    R Sharma, GK Prajapati, G Akhoury
    Israel Journal of Plant Sciences 68 (1), 124-132 2021

  • 2019-nCoV: a worldwide concern and facts
    R Sharma, G Akhoury
    VirusDisease 31 (4), 424-431 2020

  • Crop Improvement and Applied Nanobiotechnology
    R Sharma, S Prasad
    Nanotechnology: Advances and Real-Life Applications, 235 2020

  • In vitro analysis of gallstone formation in the presence of bacteria
    R Sharma, SG Sachan, SR Sharma
    Indian journal of Gastroenterology 39, 473-480 2020

  • Analysis of gallstone composition and structure in Jharkhand region
    R Sharma, S Soy, C Kumar, SG Sachan, SR Sharma
    Indian Journal of Gastroenterology 34, 29-37 2015

  • Consumption of heme iron: A major factor in pigment gallstone formation
    R Sharma, K Ujwal, JN Kishore, SG Sachan, SR Sharma
    International Journal of Biomedical Research 5 (1), 34-37 2014

  • Preponderance of gallstones in females
    R Sharma, SG Sachan, SR Sharma
    World Journal of Pharmacy and Pharmaceutical Sciences 2 (6), 5871-77 2013

  • Urease positive and slime producing bacterial activity: results in gallstone precipitation and solidification
    R Sharma, SR SHARMA, SG SACHAN
    Archives of Clinical Infectious Diseases 8 (4), 1-7 2013

  • Probing into the prevalence and factors of gallstones formation in Jharkhand Region in India.
    R Sharma, A Kumar, NK Jha, SG Sacchan, A Sharan, SRS Vidyarthi
     2012

MOST CITED SCHOLAR PUBLICATIONS

  • N-glycosylation, a leading role in viral infection and immunity development.
    VK Pandey, R Sharma, GK Prajapati, TK Mohanta, AK Mishra
    Mol Biol Rep 2022
    Citations: 37

  • Analysis of gallstone composition and structure in Jharkhand region
    R Sharma, S Soy, C Kumar, SG Sachan, SR Sharma
    Indian Journal of Gastroenterology 34, 29-37 2015
    Citations: 32

  • A comprehensive review on nanopesticides and nanofertilizers—A boon for agriculture
    B Sharma, U Lakra, R Sharma, SR Sharma
    Nano-enabled Agrochemicals in Agriculture 1, 1-18 2022
    Citations: 27

  • In vitro analysis of gallstone formation in the presence of bacteria
    R Sharma, SG Sachan, SR Sharma
    Indian journal of Gastroenterology 39, 473-480 2020
    Citations: 18

  • Preponderance of gallstones in females
    R Sharma, SG Sachan, SR Sharma
    World Journal of Pharmacy and Pharmaceutical Sciences 2 (6), 5871-77 2013
    Citations: 17

  • Probing into the prevalence and factors of gallstones formation in Jharkhand Region in India.
    R Sharma, A Kumar, NK Jha, SG Sacchan, A Sharan, SRS Vidyarthi
     2012
    Citations: 6

  • A perspective on varied fungal virulence factors causing infection in host plants
    P Kumar, R Sharma, K Kumar
    Molecular Biology Reports 51 (1), 392 2024
    Citations: 5

  • 2019-nCoV: a worldwide concern and facts
    R Sharma, G Akhoury
    VirusDisease 31 (4), 424-431 2020
    Citations: 5

  • Pentagalloylglucose, a phytochemical from Terminalia chebula can efficiently prevent SARS-CoV-2 entry: In Silico study
    R Sharma, GK Prajapati, G Akhoury
    Israel Journal of Plant Sciences 68 (1), 124-132 2021
    Citations: 4

  • Urease positive and slime producing bacterial activity: results in gallstone precipitation and solidification
    R Sharma, SR SHARMA, SG SACHAN
    Archives of Clinical Infectious Diseases 8 (4), 1-7 2013
    Citations: 4

  • Novel Microbial Compounds as a Boon in Health Management
    SR Sharma, R Sharma, D Kar
    Biotechnological Advances for Microbiology, Molecular Biology, and 2022
    Citations: 2

  • Consumption of heme iron: A major factor in pigment gallstone formation
    R Sharma, K Ujwal, JN Kishore, SG Sachan, SR Sharma
    International Journal of Biomedical Research 5 (1), 34-37 2014
    Citations: 2

  • Dysbiosis and its Varied Impacts
    R Sharma, K Kumar
    The Gut Microbiota in Health and Disease, 41-54 2023
    Citations: 1

  • Dealkenylation of neoandrographolide, a phytochemical from Andrographis paniculata stimulates FXR (Farnesoid X Receptor) and enhances gallstone dissolution
    R Sharma, K Kumar, K Tanvi
    Journal of Biomolecular Structure and Dynamics 41 (8), 3339-3348 2023
    Citations: 1

  • Impact of oxalate on the physicochemical properties of potato starch: An insight closely associated with Indian cuisine
    K Kumar, R Sharma, A Kumar, S Singh
    International Journal of Gastronomy and Food Science 31, 100660 2023
    Citations: 1

  • Role of Probiotics in Treatment of Gut‐Related Diseases
    K Kumar, R Sharma
    Prebiotics and Probiotics in Disease Regulation and Management, 1-26 2022
    Citations: 1

  • Microbial Aspect in Wastewater Management: Biofilm.
    R Sharma, SR Sharma
    Advanced Industrial Wastewater Treatment and Reclamation of Water., 71-86 2021
    Citations: 1