Ahmed Madi W. Al-Mayahi

Scopus Publications

The effect of 24-epibrassinolide and plant growth regulators on in vitro propagation, biochemical changes and genetic stability of Phoenix dactylifera 'Barhee'
Ahmed Madi Waheed Al-Mayahi
Plant Biosystems, 2026
Rooting of aerial offshoots of date palm (Phoenix dactylifera L.) “Braim” by air layering method
Ahmed Madi Waheed Al-Mayahi
Journal of Horticultural Science and Biotechnology, 2026
EFFECT OF SODIUM NITROPRUSSIDE WITH PLANT GROWTH REGULATORS ON IN VITRO PROPAGATION AND GENETIC STABILITY OF ‘BARHEE’ DATE PALM (PHOENIX DACTYLIFERA L.)
Ahmed Madi Waheed Al-Mayahi
Journal of Horticultural Research, 2025
Date palm micropropagation still faces many limitations in practical applications due to tissue browning, reduced callus growth and development, low organogenesis and multiplication efficiency, and low rooting rate. This study investigated the effects of sodium nitroprusside (SNP), a source of nitric oxide and plant growth regulators, on the growth attributes and some biochemical constituents of in vitro cultured ‘Barhee’ date palm. The addition of SNP to the culture media was more effective than the individual application of growth regulators regarding callus growth, shoot regeneration, and the number of shoots per jar. The best results (338 mg callus, 80% shoot regeneration, and 17.5 shoots per jar) were obtained in the medium with the addition of 20 μM SNP, plus 90.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for callus growth, and 17.7 μM 6-benzylaminopurine (BAP) for shoot regeneration. The above treatment reduced the amount of soluble phenolic compounds in the callus, reducing browning. Effective micropropagation was accompanied by an increase of chlorophyll content and a decrease in the levels of malondialde-hyde (MDA) and hydrogen peroxide (H2O2). The use of 20 μM SNP in combination with 5.37 μM naphthalene acetic acid (NAA) was most effective on root induction and the number of roots per shoot (93.34% and 7.14 roots per shoot). Monomorphic banding patterns obtained in RAPD-PCR and ISSR-PCR in tissue culture-derived and parental plants confirmed genetic stability. Based on these results, nitric oxide can be considered as an intermediary of callus stimulation, adventitious shoot regeneration, and root induction.
Micropropagation of Phoenix dactylifera L using phloroglucinol, melatonin and indole-3-acetic acid
Ahmed Madi Waheed Al–Mayahi
Vegetos, 2025
STIMULATING EFFECT OF CEFOTAXIME AND SILVER NANOPARTICLES ON PLANT REGENERATION IN DATE PALM TISSUE CULTURE
Ahmed Madi Waheed Al-Mayahi
Journal of Horticultural Research, 2024
The essential challenges in practical applications of date palm micropropagation include explant browning, reduced callus growth, low multiplication rate, and frequent tissue contamination. Our study aimed to evaluate the effects of cefotaxime (Cefo) and silver nanoparticles (AgNPs) on microbial contamination removal, callus growth, and shoot regeneration in date palm micropropagation. Cultures were initiated from bud tips of ‘Barhee’ date palm. Murashige and Skoog medium with auxins, cytokinins, and activated charcoal was amended with Cefo (50, 100, and 200 mg·l−1), AgNPs (0.125 and 0.250 mg·l−1), and a combination of both. The medium supplemented with 200 mg·l−1 Cefo and 0.250 mg·l−1 AgNPs resulted in the best callus growth (318 mg). In contrast, 200 Cefo + 0.125 AgNPs resulted in maximum organogenesis and shoot number per jar (83.4% and 14.1 shoots per jar, respectively). These two combinations resulted in contaminant-free cultures. The total amount of phenolic compounds was significantly reduced to 0.79 and 0.57 mg GAE·g−1 DM in shoots cultured in the above media, which was reflected in the low browning rate. The data revealed that the maximum endogenous IAA content of shoots (2.681 μg·g−1 and 2.345 μg·g−1) was obtained in response to 200 mg·l−1 Cefo + 0.250 AgNPs and 200 mg·l−1 Cefo + 0.125 mg·l−1 AgNPs, respectively. Therefore, the optimized compositions established in the present study could be applicable in reducing contamination and helping callus production and multiple shoot regeneration. To our knowledge, this is the first study of the antibacterial and growth-promoting effects of Cefo in combination with AgNPs in in vitro cultures of ‘Barhee’ date palm.
EFFECT OF ANCYMIDOL AND PHLOROGLUCINOL ON THE NUMBER AND THE QUALITY OF SHOOTS IN THE MICROPROPAGATION OF DATE PALM (PHOENIX DACTYLIFERA L.)
Ahmed Madi Waheed Al-Mayahi
Journal of Horticultural Research, 2024
The disadvantages of practical date palm micropropagation are tissue browning, low callus proliferation rate, low multiplication efficiency, and vitrification. The aim of the study was to determine the effect of ancymidol (Ancy) and phloroglucinol (PG) on the growth and some biochemical components of the ‘Barhee’ date palm cultured in vitro. The combination of 0.75 mg·l−1 Ancy and 50 mg·l−1 PG was found to be the most effective in terms of callus regeneration rate (89%) and number of shoots (14.3). A reduction in browning was observed in tissues cultured on media supplemented with 0.75 mg·l−1 Ancy in combination with 25 or 50 mg·l−1 PG. The medium supplemented with 0.75 mg·l−1 Ancy and 50 mg·l−1 PG eliminated shoot vitrification. Effective micropropagation was associated with increased carbohydrate and protein content. In this study, the genetic stability of plants obtained by micropropagation was confirmed by DNA-based RAPD fingerprinting. The results may indicate that the micropropagation protocol used in this study was suitable and applicable to the production of genetically stable date palm plants on a mass scale.
Triacontanol ‘TRIA’ application to mitigate the adverse effects of drought and salinity stress under in vitro culture of date palm plants
Ahmed Madi Waheed Al-Mayahi
Folia Oecologica, 2024
This study was conducted to attempt adaptation to combined drought and salt stresses (DS) (PEG- 6000 + NaCl) in date palm cv. Barhee implanted in vitro, keeping in mind the detrimental influence of DS. In vitro experimentation was executed on P. dactylifera L. to examine the efficacy of the application of triacontanol (TRIA), on growth attributes, and some biochemical constituents under DS. The optimal treatment was 10 µg l–1 TRIA. Such treatment under DS improved the callus growth and increased its weight to 215.0 mg. This treatment also showed the highest response rate and the number of shoots per jar (72.23% and 10.30 shoots, respectively) under DS stress. TRIA enhanced DS tolerance by increasing the contents of osmoregulatory substances such as proline, total soluble carbohydrates, and total soluble proteins, were obtained by adding 20 and 10 mg l–1 TRIA. This treatment was also more effective under DS in increasing Ca2+, Mg2+, and K+, as well as Fe2+, and chlorophyll pigment. These results also indicate that using 10 µg l–1 TRIA as a supplement under DS can increase SOD, APX, and PAL activity, to 31.68, 3.377 unit g–1 min–1, and 33.78%, respectively. Data analysis also indicated that the application of 10 µg l–1 TRIA countered the DS-induced harmful effects by reducing the content of malondialdehyd (MDA) and H2O2 in stressed tissues to 1.06, and 1.278 μMg of fresh weight (FW). Our work could reveal detailed changes in the quantity and number of protein bands by SDS-PAGE. New protein bands appeared in both stressed with TRIA-treated plants. The result of the present study will be useful for rapid clonal propagation of date palm which can be used to enhance the tolerance of plants to drought and salt stress.
Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee
Ahmed Z.R. Al-Asadi, Ahmed M.W. Al-Mayahi, Khairullah M. Awad
Folia Oecologica, 2024
The investigation was carried out to evaluate the influence of the dicamba (3,6-Dichloro-2-methoxybenzoic acid) (DIC) and casein hydrolysate (CH) on the callus growth, shoot multiplication, and some biochemical constituents of date palm cv. Barhee cultured in vitro. Both DIC and CH were required for callus growth and shoots regeneration. The medium supplemented with 4.0 mg l−1 DIC in combination with 1.0 g l−1 CH gave the highest callus weight (287 mg), while the maximum response rate and the number of shoots per jar (86.67% and 15.07 shoots/jar) were found in MS media equipped with 4 mg l−1 DIC and 0.5 mg l−1 CH combination. The total amount of phenolic compounds was significantly reduced to 0.82 and 0.79 mg GAE g–1 in shoots cultured in the medium equipped with 4.0 mg l−1 DIC with 0.5 and 1.0 g l−1 CH, which is reflected in the rate of browning. The results showed that the highest shoots content of endogenous IAA (3.71 and 3.50 μg g−1), were obtained in response to 4 mg l−1 DIC + 1.0 g l−1 CH and 4.0 mg l−1 DIC + 0.5 g l−1 CH, respectively. The macronutrient K, P, Ca, and free amino acids content significantly increased in the in vitro shoots regenerated on the media supplemented with 4.0 mg l−1 DIC + 1.0 g l−1 CH. The genetic stability of this study was confirmed by the DNA-based fingerprinting method RAPD. The RAPD binding patterns indicated no variation among tissue culture-derived plants. The in vitro propagation protocol described herein can be introduced to the production of genetically stable date palm plants.
Combined efficiency of iron nanoparticles (IONPs) and salicylic acid (SA) on in vitro propagation of date palm (Phoenix dactylifera L.) under combined drought and salinity
Ahmed Madi Waheed Al–Mayahi
South African Journal of Botany, 2023
The Effect of Phenyl Acetic Acid (PAA) on Micropropagation of Date Palm Followed by Genetic Stability Assessment
Ahmed Madi Waheed Al-Mayahi
Journal of Plant Growth Regulation, 2022
In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations
Ahmed Madi Waheed Al-Mayahi
Journal of Genetic Engineering and Biotechnology, 2022
The effect of polyamines and silver thiosulphate on micropropagation of date palm followed by genetic stability assessment
Ahmed Madi Waheed Al-Mayahi
World Journal of Microbiology and Biotechnology, 2022
In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
Ahmed Madi Waheed Al-Mayahi
Journal of Genetic Engineering and Biotechnology, 2021
The effect of humic acid (HA) and zinc oxide nanoparticles (ZnO-NPS) on in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Quntar
Ahmed Madi Waheed Al-Mayahi
Plant Cell Tissue and Organ Culture, 2021
Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv.
Ahmed Madi Waheed Al-Mayahi, Abdulminam Hussian Ali
Folia Oecologica, 2021
Effect of glutathione (GSH) on Date palm (Phoenix dactylifera L.) micropropagation
Ahmed Madi Waheed Al-Mayahi, Osama Nazim Jafar, Khaun Ali Mohsen
Folia Oecologica, 2020
Effect of Calcium and Boron on Growth and Development of Callus and Shoot Regeneration of Date Palm Barhee
Ahmed Madi Waheed Al-Mayahi
Canadian Journal of Plant Science, 2020
In vitro Assessment of ZnO Nanoparticles on Phoenix dactylifera L. Micropropagation
Khairullah Awad
Scientific Journal of King Faisal University, 2020
Effect of aluminum on the growth of the in vitro culture tissues of the date palm (Phoenix dactylifera L.) cv. Um-Aldehin
Ahmed Madi Waheed Al-Mayahi
Folia Oecologica, 2019
The effect of thidiazuron on callus and in vitro shoots development of date palm (Phoenix dactylifera L.) cv. barhee
Ahmed Z. Al-Asadi, Aqeel H. Abdulwahid, Ahmed M.W. Al-Mayahi
Basrah Journal of Agricultural Sciences, 2019
Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv. ‘Barhee’
Ahmed Madi Waheed Al-Mayahi, Abdulminam Hussien Ali, Hussein J. Shareef
Journal of Genetic Engineering and Biotechnology, 2018
Effect of red and blue light emitting diodes “CRB-LED” on in vitro organogenesis of date palm (Phoenix dactylifera L.) cv. Alshakr
Ahmed Madi Waheed Al-Mayahi
World Journal of Microbiology and Biotechnology, 2016
Influence of salicylic acid (SA) and ascorbic acid (ASA) on in vitro propagation and salt tolerance of date palm (Phoenix dactylifera L.) cv. 'Nersy'
Ahmed Madi Waheed AL-Mayahi and
Australian Journal of Crop Science, 2016
Effect of copper sulphate and cobalt chloride on growth of the in vitro culture tissues for date palm (Phoenix dactylifera l.) cv. Ashgar
AL-Mayahi
American Journal of Agricultural and Biological Science, 2013

RECENT SCHOLAR PUBLICATIONS

The effect of 24‑epibrassinolide and plant growth regulators on in vitro propagation, biochemical changes and genetic stability of Phoenix dactylifera ’Barhee’
AMW Al-Mayahi
Plant Biosystems 160 (91), - , 2026
2026
Peer Review of “Relationship Between Seed Coat Color and Cytokinin Concentration in Efficiently Regenerating Leaf Lettuce Shoots: In Vitro Experimental Study”
AMW Al-Mayahi
JMIRx Bio 4, e89401 , 2026
2026
Citations: 1
Rooting of aerial offshoots of date palm (Phoenix dactylifera L.) “Braim” by air layering method
AMW Al-Mayahi
Journal of Horticultural Science and Biotechnology 100 (4), - , 2026
2026
Citations: 1
Micropropagation of Phoenix dactylifera L using phloroglucinol, melatonin and indole-3-acetic acid
AMW Al-Mayahi
Vegetos 38 (4), - , 2025
2025
EFFECT OF SODIUM NITROPRUSSIDE WITH PLANT GROWTH REGULATORS ON IN VITRO PROPAGATION AND GENETIC STABILITY OF ‘BARHEE’ DATE PALM (PHOENIX DACTYLIFERA L.)
AMW Al-Mayahi
Journal of Horticultural Research 33 (1) , 2025
2025
Citations: 3
STIMULATING EFFECT OF CEFOTAXIME AND SILVER NANOPARTICLES ON PLANT REGENERATION IN DATE PALM TISSUE CULTURE
AMW Al-Mayahi
Journal of Horticultural Research 32 (2) , 2024
2024
Citations: 1
EFFECT OF ANCYMIDOL AND PHLOROGLUCINOL ON THE NUMBER AND THE QUALITY OF SHOOTS IN THE MICROPROPAGATION OF DATE PALM (PHOENIX DACTYLIFERA L.)
AMW Al-Mayahi
Journal of Horticultural Research 32 (2) , 2024
2024
Citations: 5
Triacontanol ‘TRIA’ application to mitigate the adverse effects of drought and salinity stress under in vitro culture of date palm plants
AMW Al-Mayahi
FOLIA OECOLOGICA 51 (2), 252-264 , 2024
2024
Citations: 8
Anatomical Study Of Adventitious Bud Regeneration From Shoot Tip Of Date Palm (Phoenix dactylifera L.) C.V Barhee In Vitro
AAAS Ahmed M. W. Al-Mayahi, Yahya N. Kalaf, Azhar M. Abdul-Sahib, Ibtisam M ...
Basrah Journal of Date Palm Research 23 (1), 116-126 , 2024
2024
Citations: 2
Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee
AZR Al-Asadi, AMW Al-Mayahi, KM Awad
Folia Oecologica 51 (1), 56-65 , 2024
2024
Citations: 14
Combined efficiency of iron nanoparticles (IONPs) and salicylic acid (SA) on in vitro propagation of date palm (Phoenix dactylifera L.) under combined drought and salinity
AMW Al-Mayahi
South African Journal of Botany 162 (-), 324-333 , 2023
2023
Citations: 14
Effect of medium type, TDZ, PG, and their interactions on In Vitro Regeneration (Phoenix dactylifera L.) cv. Barhee
AHA Ahmed Z.R. Al- Asadi, Ahmed M.W. Al-Mayahi
Basrah Journal For Date Palm Research 22 (1), 32-51 , 2023
2023
Citations: 1
In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations
AMW Al-Mayahi
Journal of Genetic Engineering and Biotechnology 20 (165), 1-10 , 2022
2022
Citations: 24
The effect of polyamines and silver thiosulphate on micropropagation of date palm followed by genetic stability assessment
AMW Al-Mayahi
World Journal of Microbiology and Biotechnology 38 (124), 1-12 , 2022
2022
Citations: 12
The Effect of Phenyl Acetic Acid (PPA) On Micropropagation of Date Palm Followed By Genetic Stability Assessment
A Al-Mayahi
Journal of Plant Growth Regulation , 2022
2022
Citations: 19
Effect of Paclobutrazol and potassium on growth
IMAS Azhar M. Abdul-Sahib, Ahmed M. W. Al-Mayahi,Mahmood Sh. Hashim
Basrah Journal For Date Palm Research 20 (2), 91-104 , 2021
2021
In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
A Al-Mayahi
Journal of Genetic Engineering and Biotechnology 19 (1), 1-9 , 2021
2021
Citations: 37
Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv.
AMW Al-Mayahi, AH Ali
Folia oecologica 48 (1), 110-117 , 2021
2021
Citations: 29
The effect of humic acid (HA) and zinc oxide nanoparticles (ZnO-NPS) on in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Quntar
A Al-Mayahi
Plant Cell, Tissue and Organ Culture , 2021
2021
Citations: 42
Effect of phloroglucinol (PG) on in vitro growth and multiplication of the date palm cv. Barhee
AZ Al-Asadi, AMW Al-Mayahi, AH Abdulwahid
Basrah Journal of Date Palm Research 14 (1), 31-45 , 2020
2020
Citations: 7

MOST CITED SCHOLAR PUBLICATIONS

Plantlet regeneration from root segments of Date palm tree (Phoenix dactylifera L. cv. Barhee) producing by in vitro culture
HAAT Majid A. Ibrahim, Ahmed M. W. Almayahi
Advances in Agriculture & Botanics 5 (1), 45-50 , 2013
2013
Citations: 51
Effect of red and blue light emitting diodes “CRB-LED” on in vitro organogenesis of date palm ( Phoenix dactylifera L.) cv. Alshakr
AMW Al-Mayahi
World Journal of Microbiology and Biotechnology 32 (10), 160 , 2016
2016
Citations: 46
The effect of humic acid (HA) and zinc oxide nanoparticles (ZnO-NPS) on in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Quntar
A Al-Mayahi
Plant Cell, Tissue and Organ Culture , 2021
2021
Citations: 42
Effect of silicon (Si) application on Phoenix dactylifera L. growth under drought stress induced by polyethylene glycol (PEG) in vitro
AMW Al-Mayahi
American Journal of Plant Sciences 7 (13), 1711-1728 , 2016
2016
Citations: 42
Thidiazuron-induced in vitro bud organogenesis of the date palm ( Phoenix dactylifera L.) CV. Hillawi
AMW Al-Mayahi
African Journal of Biotechnology 13 (35) , 2014
2014
Citations: 39
In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources
A Al-Mayahi
Journal of Genetic Engineering and Biotechnology 19 (1), 1-9 , 2021
2021
Citations: 37
Effect of copper sulphate and cobalt chloride on growth of the in vitro culture tissues for date palm (Phoenix dactylifera L.) cv. Ashgar
AMW Al-Mayahi
American Journal of Agricultural and Biological Sciences 9 (1), 6 , 2014
2014
Citations: 36
Effect of calcium and boron on growth and development of callus and shoot regeneration of date palm cv. Barhee.
AMW Al-Mayahi
Canadian Journal of Plant Science 100 (4), 357-364 , 2020
2020
Citations: 35
Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv.
AMW Al-Mayahi, AH Ali
Folia oecologica 48 (1), 110-117 , 2021
2021
Citations: 29
An efficient protocol for indirect somatic embryogenesis and shoot organogenesis from leaf segments of date palm (Phoenix dactylifera L.) CV. Quntar
AMW Al-Mayahi
African Journal of Agricultural Research 10 (10), 1031-1042 , 2015
2015
Citations: 27
Isolation and identification of associated fungi with the micropropagation of five different date palm cultivars and the effect of Benlate fungicides in their control
AAAK AM Al-Mayahi, AN Ahmed
Basra J. Date Palm Res 9 (2), 79-97 , 2010
2010
Citations: 27
Effect of aluminum on the growth of the in vitro culture tissues of the date palm (Phoenix dactylifera L.) cv. Um-Aldehin
A Al-Mayahi
FOLIA OECOLOGICA 46 (2), 164-169 , 2019
2019
Citations: 26
In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations
AMW Al-Mayahi
Journal of Genetic Engineering and Biotechnology 20 (165), 1-10 , 2022
2022
Citations: 24
In vitro Assessment of ZnO Nanoparticles on Phoenix dactylifera L. Micropropagation
KM Awad, AMW Al-Mayahi, MA Mahdi, ASM Al-Asadi, MH Abass
Scientific Journal of King Faisal University (Basic and Applied Sciences) 21 … , 2020
2020
Citations: 24
Influence of salicylic acid (SA) and ascorbic acid (ASA) on in vitro propagation and salt tolerance of date palm (Phoenix dactylifera L.) cv. ‘Nersy
AMW Al-Mayahi
Australian Journal of Crop Science 10 (7), 969-976 , 2016
2016
Citations: 24
Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv.‘Barhee’
AMW Al-Mayahi, AH Ali, HJ Shareef
Journal of Genetic Engineering and Biotechnology 16 (2), 607-612 , 2018
2018
Citations: 22
Propagation of four rare cultivars of date palm (Phoenix dactylifera L.) by tissue culture techniques
AM Jasim, AMW Al-Mayahi, AHM Attaha
Basrah Journal for Date Palm Research 8 (1), 72-99 , 2009
2009
Citations: 20
The Effect of Phenyl Acetic Acid (PPA) On Micropropagation of Date Palm Followed By Genetic Stability Assessment
A Al-Mayahi
Journal of Plant Growth Regulation , 2022
2022
Citations: 19
Effect of glutathione (GSH) on Date palm (Phoenix dactylifera L.) micropropagation
KA Al-Mayahi, A.M.W., Jafar, O.N., Mohsen
FOLIA OECOLOGICA 47 (1), 64-69 , 2020
2020
Citations: 19
The effect of thidiazuron on callus and in vitro shoots development of date palm (Phoenix dactylifera L.) cv. Barhee
AZ Al-Asadi, AH Abdulwahid, AMW Al-Mayahi
Basrah Journal of Agricultural Sciences 32, 258-265 , 2019
2019
Citations: 17

Ahmed Madi W. Al-Mayahi

RESEARCH INTERESTS

FUTURE PROJECTS

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS

Ahmed Madi W. Al-Mayahi

RESEARCH INTERESTS

FUTURE PROJECTS

Combined efficiency of iron nanoparticles (IONPs) and salicylic acid (SA) on in vitro propagation of date palm (Phoenix dactylifera L.) under combined drought and salinity

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS