Jay Prakash Pandey

@bitmesra.ac.in

Professor Dept. of chemistry
Birla institute of Technology, Mesra, India



              

https://researchid.co/jppandey

RESEARCH INTERESTS

Graft copolymer
Microwave assisted polymerization
Flocculation

66

Scopus Publications

Scopus Publications


  • Antheraea mylitta cocoonase: An explorable protease
    A. Rani, , J.P. Pandey, K. Jena, K. Sathyanarayana, D.M. Pandey, , , , and
    Triveni Enterprises
    Aim: The study was conducted to explore the insect secreted protease Antheraea mylitta cocoonase. Methodology: The cocoons of eleven ecoraces were collected followed by observation of cocoonase secretion behaviour, cocoonase collection, purification, and its quantification. In addition, the thermal stability of cocoonase and gel diffusion assay were investigated. Results: Antheraea mylitta cocoons of eleven ecoraces were collected from different eco-pockets of India. Subsequently, the cocoonase secretion behaviour of different ecoraces during emergence was studied. It was observed that the quantity and concentration of cocoonase secreted depends upon various factors such as cocoon size, cocoon stiffness, and the habitat in which silkworm is reared. To observe the proteolytic specificity of A. mylitta cocoonase, a gel diffusion assay was conducted using casein and gelatin as probable substrates. The presence of hydrolytic ring confirmed protease activity. In addition, the thermal stability of cocoonase was also observed by protease activity. Interpretation: The present study concludes that Antheraea mylitta cocoonase is an explorable protease that needs further exploration to unveil the unspecified properties of this insect-secreted protease, which can find beneficial applications in silk and other industries. Key words: Antheraea mylitta, Cocoonase, Ecoraces, Gel diffusion assay, Temperature stability

  • Mechanism of adult emergence in Xanthopimpla pedator (Fabricius) - a major pupal parasitoid of Tasar silkworm
    H. Gadad, , D.I.G. Prabhu, A. Bhagat, J. Singh, V. Mittal, J.P. Pandey, S. Kutala, , ,et al.
    Triveni Enterprises
    Aim: The study was conducted to elucidate the mechanism involved in adult emergence behavior of Xanthopimpla pedator a pupal parasitoid of tasar silkworm. Methodology: Behavioral observations were made to study the mechanical approaches used during emergence and SDS-PAGE analysis was carried to know the possibility of proteases in the digestive secretions. Results: The findings of the study suggest that X. pedator uses physical and chemical to achieve safe passage from the host pupae and cocoons. It was observed that during the process of emergence, X. pedator breaks host pupae by means of mandibular action (by biting the pupal shell). Further, it was noticed that to make emergence hole on host cocoon X. pedator found to employ digestive secretion to soften and disintegrate the silken shell, besides it also deploys mandibles to spread out the secretion on intended place of exit hole. SDS-PAGE analysis showed the presence of series of enzymes, possibly involved in cocoon softening. Interpretation: Findings of this study forms the basis that X. pedator, a major pupal parasitoid of tasar silkworm, uses both physical and chemical approaches to achieve safe passage from the host pupae and protective silken cocoon. Key words: Digestive secretion, Mandibular action, Parasitization, Xanthopimpla pedator

  • Role of CRPs in tasar sector: Present status and future strategies
    V. Mittal, , J.P. Pandey, J. Binkadakatti, R.K. Khandai, S. Alam, K. Sathyanarayana, , , ,et al.
    Triveni Enterprises
    Aim: Employ of CRPs for utilization in tasar sericulture by needy organizations under various government schemes / developmental projects. Methodology: Community Resource Persons were nurtured under ISDS (Integrated Skill Development Scheme) of CSB and MKSP Tasar projects as a sector specialist cadre who works as specialist of knowing the cutting edge technologies of tasar culture and they will bring the theory of change further to unskilled community to build their skills and creating awareness about the new scientific practices of Tasar Sericulture and building their vision and understanding around communes. CRPs were trained collectively by PRADAN and CSB. Selection of beneficiaries, organizing them into Tasar Vikas Samities (TVSs), signing of agreement with beneficiaries, support for developing infrastructure, etc., during early period of the project, to get maximum project output and keep the tribal's interested in Tasar culture, utilization of the existing Tasar food plants in the forest areas/ raised under other schemes, Skill up-gradation, handholding support to the beneficiaries in creating activity groups were the primary activities achieved by engaging Community Resource Persons (CRP). Disease monitoring, technological support, etc., were monitored by these groups of resource person's. Results: A meticulous and customized evaluation programme of the CRPs was accomplished during 2022-23. A total of 479 CRPs nurtured under the special projects in the states of Jharkhand, Bihar, Chhattisgarh, Odisha and West Bengal, were accredited jointly by CTRTI Ranchi, Birsa Agricultural University Ranchi, KVKs and TDF-PRADAN Deoghar at field level. CRPs from 05 states covering 18 districts, 46 blocks, and 248 villages consisting 340 males & 139 females participated in evaluation & accreditation of CRPs. It was found that 36.74 percentage of CRP's had expertise in rearing activities followed by grainage and rearing (33.40%) and grainage (11.27%), however; only 8.56 % of the CRP's had their expertise in post cocoon technology. The results of assessment of Community Resource Persons (CRPs) based on different criteria revealed that the CRP's having expertise in both grainage & rearing scored 35.95 which is highest among the CRP's. Whereas lowest score of 30.65 was noticed with the CRP's who had expertise in Grainage. With respect to different criteria for assessment of the CRP's expertise in Tropical Tasar under the criteria of timeliness and sincerity; CRP's scored 7.07 out of 10 score followed by community mobilization (6.83) & on field technology support (6.79). Interpretation: As Tasar sector has attracted attention of many funding agencies in the recent past, keeping in view the need of horizontal expansion and shortage of manpower with the State Sericulture departments and others parallel institutions, CRPs can play a vital role based on their skill sets for further utilization by needy organizations under various government schemes/developmental projects. Key words: Community resource person, Extension, Tasar sector, Training

  • Protein–protein docking and molecular dynamics studies of sericin and cocoonase of silkworm: an insight for cocoon softening
    Nishi Prakash Tiwari, Jay Prakash Pandey, and Dev Mani Pandey
    Informa UK Limited
    Cocoonase is known to digest the sericin protein that encapsulates the silkworm cocoon's fibroin protein. Silk fibroin and sericin are two types of proteins that make up silk, and accounts for around 20-30% of the overall cocoon weight. The aim of the study was to see the protein-protein interaction (PPI) and molecular dynamic study of sericin, cocoonase and protein-protein docked complex of silkworm by computational approaches. Here motif analysis, phylogenetic analysis, principal component analysis, root-mean-square deviation (RMSD), root mean square fluctuation, radius of gyration, structural and functional study of cocoonase and sericin as well as molecular docking study were carried out. The 33 amino acid residues of cocoonase shows interaction with 38 aa residues of sericin involving 4 disulphide bonds, 22 hydrogen bonds and 319 non-bonded contacts. The confirmational stability and flexibility of both the proteins as well as protein-protein complex were achieved at 70 ns of MD simulation study. RMSD-based data indicated that cocoonase is more stable than sericin and complex, and complex has a greater fluctuation with more compact (higher Rg) value than cocoonase and sericin, inferring higher conformational stability and flexibility of protein-protein complex than cocoonase and sericin. This study provides a new dimension for PPI study by computational approaches.Communicated by Ramaswamy H. Sarma.

  • Evaluating the role of trypsin in silk degumming: An in silico approach
    Sneha, Jay Prakash Pandey, and Dev Mani Pandey
    Elsevier BV

  • Analysis of chemical composition and assessment of biological potential of glowing compounds extracted from an exuviae of tasar silkworm Antheraea mylitta
    K. Jena, , S. Ananta, J. Akthar, A. Patnaik, Mr. Chakrapani, A. Sinha, P.K. Kar, J.P. Pandey, K. Sathyanarayana,et al.
    Triveni Enterprises
    Aim: Analysis of chemical composition and assessment of biological potential of tasar exuviae extracts. Methodology: The biochemical components such as proteins, chitin, ash, fat, moisture, minerals and carbohydrate were estimated in the exuviae. After extraction, glowing pigments were characterized by UV-Vis and fluorescent spectra, and profiling of polyphenolic and flavonoids, free radical scavenging assay, antibacterial and anticancer potential were evaluated. Results: The proximate components such as proteins, chitin, ash, fat, moisture, and carbohydrate contents were 62, 11.52, 10.57, 3.9, 3.1 and 0.9%, respectively. Further, among different elements, calcium level was higher than other elements such as magnesium, manganese, copper, zinc and sodium. The methanolic extract exhibited free radical scavenging and anti microbial potential in concentration dependent manner. In addition to these, glowing compounds prominent cytotoxic effect against breast cancer cell lines viz. MCF7, BT474 and SKBR3. Interpretation: The action mechanism of extract could be probably due to the presence of yellow flavonoids (luteolin, catechin, epicatechin and quercetin) and phenolic acids (p-coumaric acid, gentisic acid and gallic acid), which glows during exposure of UV light and having biological potentials. Key words: Anticancer, Antheraea mylitta, Tasar exuviae




  • Study on cocoonase, sericin, and degumming of silk cocoon: computational and experimental
    Preeti Anand, Jay Prakash Pandey, and Dev Mani Pandey
    Springer Science and Business Media LLC
    Abstract Background Cocoonase is a proteolytic enzyme that helps in dissolving the silk cocoon shell and exit of silk moth. Chemicals like anhydrous Na2CO3, Marseille soap, soda, ethylene diamine and tartaric acid-based degumming of silk cocoon shell have been in practice. During this process, solubility of sericin protein increased resulting in the release of sericin from the fibroin protein of the silk. However, this process diminishes natural color and softness of the silk. Cocoonase enzyme digests the sericin protein of silk at the anterior portion of the cocoon without disturbing the silk fibroin. However, no thorough characterization of cocoonase and sericin protein as well as imaging analysis of chemical- and enzyme-treated silk sheets has been carried out so far. Therefore, present study aimed for detailed characterization of cocoonase and sericin proteins, phylogenetic analysis, secondary and tertiary structure prediction, and computational validation as well as their interaction with other proteins. Further, identification of tasar silkworm (Antheraea mylitta) pupa stage for cocoonase collection, its purification and effect on silk sheet degumming, scanning electron microscope (SEM)-based comparison of chemical- and enzyme-treated cocoon sheets, and its optical coherence tomography (OCT)-based imaging analysis have been investigated. Various computational tools like Molecular Evolutionary Genetics Analysis (MEGA) X and Figtree, Iterative Threading Assembly Refinement (I-TASSER), self-optimized predicted method with alignment (SOPMA), PROCHECK, University of California, San Francisco (UCSF) Chimera, and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) were used for characterization of cocoonase and sericin proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), protein purification using Sephadex G 25-column, degumming of cocoon sheet using cocoonase enzyme and chemical Na2CO3, and SEM and OCT analysis of degummed cocoon sheet were performed. Results Predicted normalized B-factors of cocoonase and sericin with respect to α and β regions showed that these regions are structurally more stable in cocoonase while less stable in sericin. Conserved domain analysis revealed that B. mori cocoonase contains a trypsin-like serine protease with active site range 45 to 180 query sequences while substrate binding site from 175 to 200 query sequences. SDS-PAGE analysis of cocoonase indicated its molecular weight of 25–26 kDa. Na2CO3 treatment showed more degumming effect (i.e., cocoon sheet weight loss) as compared to degumming with cocoonase. However, cocoonase-treated silk cocoon sheet holds the natural color of tasar silk, smoothness, and luster compared with the cocoon sheet treated with Na2CO3. SEM-based analysis showed the noticeable variation on the surface of silk fiber treated with cocoonase and Na2CO3. OCT analysis also exemplified the variations in the cross-sectional view of the cocoonase and Na2CO3-treated silk sheets. Conclusions Present study enlightens on the detailed characteristics of cocoonase and sericin proteins, comparative degumming activity, and image analysis of cocoonase enzyme and Na2CO3 chemical-treated silk sheets. Obtained findings illustrated about use of cocoonase enzyme in the degumming of silk cocoon at larger scale that will be a boon to the silk industry.


  • Synthesis of Diallyl dimethyl ammonium chloride grafted polyvinyl pyrrolidone (PVP-g-DADMAC) and its applications
    Anurag Mehta, J.P. Pandey, and Gautam Sen
    Elsevier BV
    Abstract The search for a sustainable and nontoxic polymeric product has fired an intense research in the field of graft copolymers. This study endeavor to create a polyacrylamide free novel flocculant which showed efficient behavior towards water and wastewater treatment and for mineral ore beneficiation. We synthesized the novel graft copolymer - Poly Diallyl Dimethyl Ammonium Chloride (DADMAC) grafted polyvinyl pyrrolidone (PVP-g-pDADMAC) via graft polymerization route by microwave assisted method. The synthesized novel cationic graft co-polymer has been characterized by elemental analysis, FTIR spectroscopy, intrinsic viscosity measurement, molecular weight determination, and scanning electron micrograph (SEM), taking PVP as reference. The effects of reaction parameters onto the percentage grafting have been studied. The applicability of these grafted polymers as flocculant for the treatment of municipal sewage waste water as well as for mineral ore beneficiation and for suppression of nanopollutant like multi-walled carbon Nano-tubes (MWCNTs) (as nano scavenger) from water systems were also investigated.

  • Graft copolymer of PVP—A sutureless, haemostatic bioadhesive for wound healing application
    Pinki Pal, Naresh Rangra, S. Samanta, Ayush Aryan, Jay Prakash Pandey, and Gautam Sen
    Springer Science and Business Media LLC
    This study includes the application of graft copolymer (polyacrylamide-grafted polyvinyl pyrrolidone—PVP-g-PAM) as wound healing agent. It is hypothesized that the branches of the graft copolymer adsorb at various colloidal components of blood plasma—thus assisting in formation of a networked structure, i.e. ‘blood clot’. Further, the adhesive property of graft copolymer supports in wound closure and immobilization of pathogens. Wound healing efficiency of PVP-g-PAM has been investigated ‘in vivo’ by excision and incision wound model. The 100% wound contraction has been shown by PVP-g-PAM after 13 days. This is superior to both in cases of standard drug and ungrafted polyvinyl pyrrolidone (PVP), which fails to achieve complete wound closure even after 21 days. Additionally, the graft copolymer-treated mice’s skin exhibited higher tensile strength than that of standard drug, PVP and stitched model.

  • Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and ‘in vitro’ drug release evaluation
    Pinki Pal, Srikant Kumar Singh, Sumit Mishra, Jay Prakash Pandey, and Gautam Sen
    Elsevier BV
    The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated. Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique. The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method. The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics. The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values. Additionally, the release rate rises with rise in pH milieu.

  • Processing techniques or mycotoxins-a balancing act of food safety and preservation
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen
    Elsevier
    Abstract Mycotoxins, the toxic metabolites of filamentous fungi have deleterious and persistent effects not only on human being but also on other vertebrates and invertebrates including plants and microorganisms. Agricultural commodities may easily get contaminated during both preharvest and postharvest. Presently, over 300 mycotoxins are identified. The International Agency for Research on Cancer (IARC) has categorized some of these toxins as carcinogenic such as aflatoxin, ochratoxins, patulin, alternaria, etc. Numerous techniques are being employed in food industries for the processing and preservation of vegetables and fruits juice. The prime objective of these techniques is the development of new products and increasing the shelf life of raw and processed food products. Prevailing trends of consumption of fruits juice have compelled companies to package fruits juice with minimal treatment, but every raw is not holistic—specially when consumed in hours or even in days after packaging. The threat of mycotoxins looms covertly behind such products. The key focus of this review is to evaluate the preservation and processing techniques and their calculated risks involved with respect to uncertainty of the presence of mycotoxins.

  • Novel Biocide Based on Cationic Derivative of Psyllium: Surface Modification and Antibacterial Activity
    Pinki Pal, Aparna Banerjee, Karuna Soren, Priyanka Chakraborty, Jay Prakash Pandey, Gautam Sen, and Rajib Bandopadhyay
    Springer Science and Business Media LLC
    To circumvent the problems (such as volatilization, photolytic decomposition, chemical instability, easy permeability through the skin) associated with low molecular weight antimicrobial agents, our strategy employed graft copolymerization of cationic monomer with the polymeric substrate viz. psyllium husk. The surface graft concentration of quaternary ammonium ion on psyllium was optimized by measuring the extent of grafting in the microwave-induced process. The surface modification of graft copolymers was evident by different physico–chemical techniques like 13C nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), X-ray diffractometer (XRD), differential scanning calorimetry (DSC), Thermo gravimeteric analysis (TGA), and viscometric study. The synthesized water soluble product resulted good antibacterial activity against Gram positive Staphylococcus aureus and Bacillus anthracis but failed to produce any antibacterial activity against Gram negative Salmonella typhi and Escherichia coli. The reason to failure has been explained on the basis of differences in the electrokinetic property between Gram-positive and Gram-negative bacteria. Optimum minimum inhibitory concentration (MIC) for the polymeric grade which showed good biocidal effect evaluated by both zone of inhibition and absorbance is found at 1000 µg/ml.

  • Free radical scavenging potential of sericin obtained from various ecoraces of tasar cocoons and its cosmeceuticals implication
    K. Jena, J.P. Pandey, Ruchi Kumari, A.K. Sinha, V.P. Gupta, and G.P. Singh
    Elsevier BV
    Tropical tasar silkworm Antheraea mylitta is a wild sericigenous insect which is distributed in different geographical regions and named as different ecoraces. In the present study, we investigated the molecular characterisation and cosmeceutical properties of sericin extracted from different ecoraces of tasar cocoons. The surface morphology and molecular weight of cocoons were determined by scanning electron microscope (SEM) and SDS-PAGE, respectively. Characterisation of sericin was performed by various methods such as FTIR, CHNS, TGA and amino acid analyzer. The anti-tyrosinase, anti-elastase, glutathione-S-transferase inhibition, free radical scavenging potential and inhibition of oxidative damages were measured in tasar ecoraces sericin. SEM images have revealed the removal of sericin from the surface of cocoons. SDS-PAGE of sericin depicted the presence of diverse molecular weight of proteins. Structural determination by FTIR revealed the presence of both α-helical and β-sheet structures. Thermal properties of sericin were studied by TGA which showed a 50% weight loss at temperature 410 °C-430 °C. Additionally, ecoraces sericin contains 17 amino acids in which serine, aspartic acid and glycine are predominantly present (55.68-59.61%). Further, anti-tyrosinase, anti-elastase, inhibition of glutathione-S-transferase activity, free radical scavenging potential and inhibition of lipid peroxidation were also observed in ecoraces sericin. Our findings suggest that the present study appear to be helpful in exploiting sericin as potential biomaterial in cosmeceutical and allied field.

  • Synthesis and optimization of hydrolyzed gum ghatti as nano-hunters – Flocculant for destabilization of nanoparticles
    Pinki Pal, Sakshi Suman, Arpita Verma, Jay Prakash Pandey, and Gautam Sen
    Elsevier BV
    Abstract This article presents a successful endeavour towards destabilization of an aqueous suspension of carbon nanoparticles via synthesized hydrolyzed graft copolymer (Hyd. GGI-g-PAM) of gum ghatti. Flocculation proficiency of the preformed graft copolymer of gum ghatti (GGI-g-PAM) has been amplified via partial alkaline hydrolysis that helps in straightening and uncoiling of the grafted monomer chains consequently increasing radius of gyration and hence the hydrodynamic volume of the molecule. Standard jar test procedure has been followed to analyze and compare the flocculation proficiency of the novel product with that of both the gum ghatti and GGI-g-PAM in coal-fine suspension as well as in aqueous suspensions of MWCNTs.

  • Synthesis and study of hydrolyzed polyacrylamide grafted polyvinyl pyrrolidone (Hyd.PVP-g-PAM) as flocculant for removal of nanoparticles from aqueous system
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen
    Elsevier BV
    Abstract This study investigates the real potential of synthesized hydrolyzed graft copolymer (Hyd.PVP-g-PAM) as flocculant in destabilization of aqueous suspension of nanoparticles (multi-walled carbon nanotubes or MWCNTs). A novel product (Hyd.PVP-g-PAM) has been synthesized by partial alkaline hydrolysis of a preformed graft copolymer of polyvinyl pyrrolidone (PVP-g-PAM). This novel product has much higher flocculation efficacy than the parent graft copolymer (PVP-g-PAM) as well as PVP, due to much larger radius of gyration as a consequence of straightening and uncoiling of the grafted chains. The flocculation competency of the product has been examined and compared with the PVP-g-PAM and with the commercial flocculant polyacrylamide (PAM) in coal-fine and in aqueous suspensions of MWCNTs through standard jar test procedure. The result indicates that the synthesized flocculant has better flocculation efficacy than PAM. The optimal dosage as flocculant of synthesized product was at 1 ppm in MWCNT suspension whereas in coal-fine at 0.75 ppm.

  • Conferring Antibacterial Properties on Sesbania Gum via Microwave-Assisted Graft Copolymerization of DADMAC
    Pinki Pal, Aparna Banerjee, Urmi Halder, Jai Prakash Pandey, Gautam Sen, and Rajib Bandopadhyay
    Springer Science and Business Media LLC
    Microbial contamination, the consequences of both anthropogenic and geogenic activity, includes high health and environmental threats and may adversely affects the ecological niche. The present work is a successful attempt towards the fabrication of anti-bacterial agent by incorporation of cationic monomer, diallyldimethylammonium chloride (DADMAC) on sesbania gum through microwave assisted method. The grafted chains of poly (DADMAC) provide positive charge to sesbania gum which in turn confers antibacterial activity. The synthesized product was characterized through FTIR, SEM, TGA, DSC elemental and viscometric analysis. The inhibitory activity of the synthesized product has been evaluated in vitro against both Gram-positive (Staphylococcus aureus NCIM 2122 and Bacillus anthracis) as well as Gram-negative (Salmonella typhi NCIM 2501 and Escherichia coli NCIM 2832) bacteria through standard agar cup method. Optimum minimum inhibitory concentration for each grade of graft copolymer measured by calculating the zone of inhibition has been found at 750 µg/ml.Graphical Abstract

  • Grafted sesbania gum: A novel derivative for sugarcane juice clarification
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen
    Elsevier BV
    This work describes the significance of graft copolymer induced flocculation process in sugarcane juice clarification. The naturally occurring cinnamic acid has been successfully incorporated into the structure of sesbania gum through microwave assisted technique (hybrid of conventional and microwave based method) to develop novel, non-toxic, eco-friendly product with highly extended structure. The customized structure of gum derivative has been characterized by a number of modern techniques like 13C NMR, FTIR, SEM, XRD, TGA, DSC, elemental and viscometric analysis. The separation of suspended colloidal impurities from cane juice has been achieved through the application of synthesized gum derivative as a flocculant. The clarification performance of the graft copolymer has been evaluated in terms of turbidity, absorbance, and degree brix (°bx). The flocculant exhibited maximum efficiency at 1.0ppm (optimized dose) without altering the nutritional parameters (% sugar content). The clarified juice thus obtained may be used as a beverage for direct consumption or for the manufacture of white sugar.

  • Tasar silk fiber waste sericin: New source for anti-elastase, anti-tyrosinase and anti-oxidant compounds
    K. Jena, J.P. Pandey, Ruchi Kumari, A.K. Sinha, V.P. Gupta, and G.P. Singh
    Elsevier BV
    The present study investigates the properties of sericin extracted from tasar silk fiber waste (TSFW). The surface morphology of TSFW was observed by scanning electron microscope (SEM). SEM images revealed the removal of residual sericin over the surface of TSFW. The molecular weight distribution of sericin was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that TSFW sericin represented a family of proteins with wide-ranging molecular weight distribution (11-245 kDa). Structural determination by FTIR revealed the presence of both α-helical and β-sheet structures. The colour was studied by colorimeter indicating less brightness, more red and yellow colour intensities. The carbon: nitrogen ratio (C:N) was studied by CHNS element analyzer and the ratio is 5.15-7.85. Thermal properties of TSFW sericin have been studied by thermogravimetric analysis (TGA) method. TGA curve showed higher thermal stability and variable degradation profiles. Furthermore, TSFW sericin contains 17 amino acids where serine, aspartic acid and glycine are the more significant compounds (54.34-60.49%). In addition, sericin was found to inhibit tyrosinase, elastase and glutathione-S-transferase activity, and had apparent radical scavenging impacts on 2.2‑diphenyl‑1‑picryl‑hydrazil (DPPH), hydrogen peroxide and inhibition of lipid peroxidation. Result suggested that TSFW sericins might be a valuable ingredient for cosmoceutical products.

  • Sesbania gum based hydrogel as platform for sustained drug delivery: An ‘in vitro’ study of 5-Fu release
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen
    Elsevier BV
    The purpose of this study is to fabricate 5-Fluorouracil sustained release matrix based on a novel, nontoxic, eco-friendly modified biopolymer. The sesbania gum based hydrogel has been prepared by microwave assisted method using acrylamide as a monomer and N,N Methylenebisacrylamide as a crosslinker. The crosslink copolymerization has been confirmed by several modern techniques such as FTIR, SEM, XRD, TGA, DSC, elemental analysis etc. The bioactive 5-Fluorouracil has been encapsulated via solvent swelling method and its release rate has been investigated in various pH dissolution medium through USP standard protocol. The synthesized hydrogel with higher degree of crosslinking exhibited slower release rate than that of hydrogel having lower degree of crosslinking. Thus, resulting higher t25 value, the release rate increases with increase in pH of the medium. Release kinetics suggests the non-Fickian release behaviour of the hydrogel.

  • Biosorptive removal of arsenite and arsenate from aqueous medium using low-cost adsorbent derived from ‘Pods of green peas’: Exploration of kinetics, thermodynamics and adsorption isotherms
    Ashish Kumar, Jayprakash Pandey, and Satish Kumar
    Springer Science and Business Media LLC
    The present work explores the biosorption characteristics of pods of green peas (PGP) for arsenite [As(III)] and arsenate [As(V)] removal from aqueous medium. Optimization of adsorption parameters like contact time, arsenic concentration, temperature, biosorbent dose and pH through batch mode of experiments was studied. Among the different isotherm models, Langmuir for As(III) and Temkin for As(V) provided the best fit for the obtained data of arsenic sorption onto PGP. D-R mean free energy (E) indicates the process is physisorption. Thermodynamics studies were found to be endothermic, feasible and spontaneous. Kinetically, adsorption follows the pseudo-second-order kinetics in sorption of As(III) and As(V) both. The desorption studies of exhausted PGP show 81% of As(III) and 72% of As(V) could be leached out. Reusability of biosorbent up to 7th cycles of incessant operation supports their commercial importance with very little effects of common ions on sorption capacity.

Publications

1. Anurag Mehata, J.P. Pandey, Gautam Sen; Synthesis of diallyl dimethyl ammonium chloride grafted polyvinyl pyrrolidone (PVP-g-DADMAC) and its applications, Material Science & Engineering B, .
2. Pinki pal, Srikant kumar singh, Sumit Mishra, Jay prakash pandey, Gautam sen; Gum ghatti based hydrogel: Microwave synthesis, characterization, 5- Fluorouracil encapsulation and ‘in vitro’ drug release evaluation, Carbohydrate polymers, .
3. Pinki pal, Naresh Ranga, S.Samanta, Ayush Aryan, Jay Prakash Pandey, Gautam Sen; Graft copolymer of PVP-A sutureless, hameostatic bioadhesive for wound healing applications, Polymer Bulletein, .
4.Priti Rani, Pinki Pal, Jay Prakash Pandey, Sumit Mishra, Gautam Sen; Alginic Acid Derivatives: Synthesis, Characterization and Application in Wastewater Treatment, Journal of polymer and environment, , .
5. Pinki pal, Aparna Banerjee, Karuna Soren,Priyanka Chakraborty, Jay Prakash Pandey, Gautam Sen, Rajib Bandopadhay; Novel Biocide Based on Cationic Derivative of Psyllium: Surface Modification and Antibacterial Activity, Journal of polymer and the environment, .
7. Pinki Pal, Jay Prakash Pandey, Gautam Sen; Synthesis and study of hydrolyzed polyacrylamide grafted polyvinyl pyrrolidone (Hyd. PVP-g-PAM) as flocculant for removal of nanoparticles from aqueous system, Material Science & Engineering B, ,