Tamil Nadu Veterinary and Animal Sciences University
Virology, Cell culture, Immunology and Molecular biology
Animal viral diseases
Thahira parveen S, Parthiban S, Raja P, Parthiban M, and Dhinakar Raj G Exploratory Animal and Medical Research, West Bengal Veterinary Alumni Association
Mohammed Al-Rasheed, Christopher Ball, Sivamurthy Parthiban, and Kannan Ganapathy Elsevier BV
S. Parthiban, A. Ramesh, Anbu Kumar Karuppannan, G. Dhinakar Raj, S. Hemalatha, M. Parthiban, K. Senthilkumar, D. Balasubramaniyam, R. Sumanth Kumar, S. Ranganatha,et al. Springer Science and Business Media LLC
Parthiban S, Ramesh A, Anbu Kumar Karuppannan, Dhinakar Raj G, Johnson Rajeswar J, Hemalatha S, Jaisree S, Senthilkumar K, Balasubramanyam D, Parthiban M,et al. Wiley
A total of 200 samples from Porcine circovirus 2 suspected (n=112) and healthy (n=88) swine populations collected from different districts of Tamil Nadu, south India were used in this study. The samples comprising of serum (n=124), swabs from natural orifices (n=52), and postmortem tissues (n=24). All the samples were processed and subjected to the screening and detection of the PCV2 genome by a specific PCR assay. PCV2 genomes from positive samples were further subjected to genotyping with specifically designed primers for the full-length amplification of the ORF2 gene which codes for capsid protein (Cp) and serves as an epidemiological marker. Randomly, thirteen amplified ORF2 genes were sequenced and the aligned sequences were subjected to signature motif analysis and phylogeny in MEGA X. The molecular prevalence of PCV2 infection in Tamil Nadu is 10.5% (n=21). Signature motif and phylogenetic studies of 13 samples revealed 38.5% (n=5) presence of each PCV2b intermediate 1(IM1) and PCV2b genotypes, followed by 15.4% (n=2) PCV2d-2 and 7.7% (n=1) PCV2d genotypes. The PCV2b-IM1 genotype has a 99.43% sequence homology with Vietnam isolate (GenBank Accession No. JX506730). PCV2b genotypes showed 99.72% sequence identity with Chinese isolate (KX068219). PCV2d-2 genotypes reported in this study has 100% sequence identity with Taiwan isolate (MF169721). PCV2d genotype showed 97.87% sequence identity with Thailand isolate (MF314293). Amino acid analysis of all the 13 full-lengths ORF2 gene sequences revealed specific mutations in the immune reactive domains of A, B, C, and D. Capsid protein of three PCV2b and five PCV2b IM1 isolates had extra amino acid residue lysine (K) at 234 position of ORF2 similar to PCV2d. For the first time in south India, PCV2b IM1 and PCV2d-2 genotypes are reported. This study evidences the genetic shifts of PCV2 isolates in India and it is analogs to that of global genotypic shift.
Parthiban S, Ramesh A, Anbu Kumar Karuppannan, Dhinakar Raj G, Hemalatha S, John Kirubaharan J, Parthiban M, Senthilkumar K, Balasubramanyam D, Sumanth Kumar R,et al. Exploratory Animal and Medical Research, West Bengal Veterinary Alumni Association
: Porcine circovirus 2 (PCV2) is the emerging viral pathogen in the swine associated with multi-systemic clinical and subclinical outcomes. This study aimed to detect molecular and serological prevalence of PCV2 infection from southern states of India. A total of 434 random samples comprising of serum (n=273), pooled postmortem tissues (n=109) and rectal, vaginal and nasal swabs (n=52) and were collected from PCV2 suspected and healthy swine populations of Tamil Nadu, Kerala, Andhra Pradesh, Telangana and Puducherry states in India during 2019 to 2021 were screened for PCV2 by specific polymerase chain reaction (PCR) assay. Of 434 samples screened, 12.2% (n=53) showed positivity to PCV2 genome. Statistical analysis of molecular prevalence of PCV2 within breed, age, sex and vaccination status revealed no significant (p>0.05) difference but there was a significant (p<0.05) difference in the prevalence of PCV2 among healthy and suspected swine populations. Suspected pigs had significantly higher prevalence of PCV2 in comparison to healthy. ELISA based PCV2 antibody screening in 176 non-vaccinated serum samples revealed sero-positivity of 44.8% (n=79). The molecular and seroprevalence of PCV2 is alarming in southern states of India, which necessitates the need for genotypic characterization and phylogenetic analysis and development of candidate vaccine for implementation of suitable prevention and control measures.
S. Parthiban, J. John Kirubaharan, A. Ramesh, M. Vidhya, S. Rajalakshmi, Ranjani Rajasekaran, and A. Thangavelu Exploratory Animal and Medical Research, West Bengal Veterinary Alumni Association
: Newcastle disease (ND) remains the most significant disease of poultry sector and contributes to huge economic loss. Early detection and pathotyping of Newcastle disease virus associated with field infection are highly crucial. In vivo pathogenicity assaying is sensitive and specific pathotyping tool used for detection and identification of NDV used until the recent past. Genome based sequence analysis yields promising results in virulence determination. Keeping the above facts, the present study was designed to compare the efficacy of conventional and molecular assays in NDV virulence determination. In this study twelve NDV isolates (Isolate numbers 463, 464, 475, 476, 122-17C, 122-17D, 122-17E, 128-17A, 128-17D, 137, 139, 141) available in the Department of Veterinary Microbiology, Madras Veterinary College (MVC), Chennai was subjected for differentiation of virulent and avirulent strains using mean death time (MDT) in specific pathogen-free (SPF) embryonated eggs and TaqMan minor groove binding (MGB) probe real-time PCR assay. Pathotyping based on the MDT revealed two NDV isolates (isolate no. 476 and 128-17D) as velogenic strains and the remaining ten NDV isolates as lentogenic strains. Pathotyping based on TaqMan MGB probe real-time PCR assay revealed six NDV isolates (476, 128-17D, 463, 464, 475, 137) as velogenic/mesogenic strains and remaining six NDV isolates (122-17C, 122-17D, 122-17E 128-17A, 139, 141) as lentogenic strains. Using a TaqMan MGB probe real-time PCR assay, four NDV isolates (463, 464, 475, 137) which were MDT pathotyped as lentogenic strains were re-pathotyped as velogenic/mesogenic strains, which indicates the greater sensitivity of TaqMan MGB probe real-time PCR assay in pathotyping of NDV over conventional MDT.
S. Parthiban, R. K. V. Sowndhraya, P. Raja, M. Parthiban, A. Ramesh, G. Dhinakar Raj, K. Senthilkumar, D. Balasubramanyam, S. Hemalatha, R. Bharathi,et al. Springer Science and Business Media LLC
S. Parthiban, A. Ramesh, G. Dhinakar Raj, Anbu Kumar Karuppannan, S. Hemalatha, M. Parthiban, Chintu Ravishankar, K. Senthilkumar, and D. Balasubramaniyam Springer Science and Business Media LLC
S. Parthiban, Hirak Kumar Mukhopadhyay, D. Panneer, P. X. Antony, and R. M. Pillai Springer Science and Business Media LLC
S. Parthiban, H. K. Mukhopadhyay, P. X. Antony, and R. M. Pillai Springer Science and Business Media LLC