DOSE DEPENDENT IMUNOMODULATORY EFFECTS OF THE HERBAL COMBINATION SAMBILOTO–GINGER–TURMERIC (SIJAKUN): AN IN VIVO STUDY ON WISTAR RATS HANDAYANI, HOTIMAH MASDAN SALIM, ARIFA MUSTIKA, AIN DAROJAH SIDDIQ RAMADHANA, RETNO DIAH PUTRI EKAYANTI International Journal of Applied Pharmaceutics, 2026 Objective: This study aimed to evaluate the effects of Sijakun at different doses on key inflammatory parameters—Neutrophil-to-Lymphocyte Ratio (NLR), macrophage count, TNF-α, and IL-6—in an LPS-induced inflammatory model. Methods: An experimental study was conducted using animal subjects randomly allocated into five groups: negative control, LPS-induced positive control, and three treatment groups receiving Sijakun at graded doses. Inflammatory markers were assessed post-intervention. Data were analyzed using ANOVA for normally distributed parameters and Kruskal–Wallis for non-normal data, followed by appropriate post hoc tests. Results: Sijakun administration significantly influenced all measured parameters (p<0.05). The highest dose (500 mg/kg BW) most effectively reduced NLR, TNF-α, and IL-6 levels, bringing them close to baseline control values. In contrast, the medium dose (250 mg/kg BW) optimally increased macrophage count, indicating enhanced innate immune response. Conclusion: Sijakun exerts significant anti-inflammatory and immunomodulatory effects in a dose-dependent manner. A high dose (500 mg/kg BW) is most effective in suppressing systemic and cytokine-mediated inflammation, while a medium dose (250 mg/kg BW) best enhances macrophage-mediated phagocytosis. These findings support the potential of Sijakun as a complementary herbal agent for modulating LPS-induced inflammatory responses.
Ultrasound-Based Knee Osteoarthritis Severity Assessment and Its Association With Kellgren–Lawrence Grading Rita Vivera Pane, Handayani, Aisyah, Nurhasyimah Hisamud-Din, Aufar Zimamuz Zaman Al Hajiri, et al. Scientific World Journal, 2026 Background Kellgren–Lawrence (KL) grading is a conventional radiography (CR)‐based system commonly used to assess osteoarthritis (OA). Knee OA is characterized by damage to the femoral cartilage (FC); however, CR cannot directly visualize cartilage integrity. Ultrasound (US) may be superior in detecting FC damage. This study is aimed at evaluating the US‐based knee OA severity assessment and analyze its association with KL grading. Methods This was an analytical observational study with a cross‐sectional design which included knee OA patients. All participants underwent knee US scanning by two independent raters. The severity of knee OA was assessed based on three parameters: the contour of the femoral cartilage, the presence of osteophytes, and meniscus protrusion. The results were accumulated and interpreted into grades from 0 to 4. Association between US‐based knee OA assessment and KL grading was analyzed statistically. Results A total of 112 knee OA patients were included in this study. The average age of participants was 61.4 years, with a higher female prevalence. Grade 3 was the most frequently recorded in both US and KL grades. The US interpretation from both raters showed a perfect agreement based on weighted kappa analysis ( κ = 1.00). A chi‐square test showed a significant association between the US and KL grade ( p < 0.001), with a very strong association based on the Cramér′s V test of 0.742 (95% CI: 0.599–0.824). Conclusion This study demonstrates the US‐based knee OA severity assessment and was associated with KL grade.
Effect of Bay Leaf (Syzygium polyanthum) Extract on Antioxidant Activity, MDA Levels, and Liver Histopathology Feature of Ethambutol Induced Wistar Rats Bambang Edi Suwito, Lea Maera Shanty, Retna Gumilang, Handayani Handayani, Renata Alya Ulhaq Indonesian Journal of Medical Laboratory Science and Technology, 2022 Bay leaf extract (Syzygiun polianthum) is one herbal elemnt that may be used to lessen liver function issues, lessen symptops of nausea, vomiting, discomfort and improve adherence and the effectiveness of tuberculossi treatment. The objective of this study was to determine the effect of bay leaf (Syzygium polyanthum) extract on antioxidant activity, Malondialdehyde (MDA) levels, and liver histopathology of ethambutol-induced Wistar rats. This research is a laboratory experiment. Antioxidant activity of Bay Leaf Extract was evaluated by comparing vitamin C with spectrophotometry methods. White Wistar rats were separated into 6 groups and uset to test the lebel of MDA and livers histopathology. Group 1 serves as the control group and received DMSO (placebo); Group 2 was received ethambutol 50mg/kg BW; Group 3 was received ethambutol and silymarine with dose 50mh/kg BW. Group 4-6 had been given ethambutol and extra ethanol extract of bay leaves of 75,150, dan 300mg/kg BW, respectively. The results of the spectrophotometry showed that the Bay Leaf Extract had antioxidant activity comparable to that of vitamin C, with an IC50 of 11.4 g ± (4.4%). One-Way ANOVA test results obtained p = 0.002 (p<0.05) while the Kruskal-Wallis test results obtained the Asymp. Sig value 0.105> 0.05. There was a significance difference in each group’s MDA levels (p=0.002). Although there was no significant difference in the liver histopathology of treated rats (p>0.05). While bay leaf extract significantly lowers MDA levels in ethambutol-induced Wistar rats, it has no discernible impact on the liver histopathology of ethambutol-induced Wistar rats. Bay leaf extract possesses antioxidant activity comparable to vitamin C.
Impact on Medical Education and the Medical Student’s Attitude, Practice, Mental Health, After One Year of the Covid-19 Pandemic in Indonesia Yuda Turana, Putu Asih Primatanti, Wawang S. Sukarya, Marwito Wiyanto, Artha Budi Susila Duarsa, et al. Frontiers in Education, 2022 IntroductionThe COVID-19 pandemic has caused disruptions in educational institutions across the country, prompting medical schools to adopt online learning systems. This study aims to determine impact on medical education and the medical student’s attitude, practice, mental health after 1 year of the Covid-19 pandemic in Indonesia.MethodsThis study utilized a cross-sectional design. An online questionnaire was distributed digitally to 49 medical schools in Indonesia from February–May 2021. A total of 7,949 medical students participated in this study. Sampling was carried out based on a purposive technique whose inclusion criteria were active college students. This research used questionnaires distributed in online version among 49 medical faculties that belong to The Association of Indonesian Private Medical Faculty. Instruments included demographic database, medical education status, experience with medical tele-education, ownership types of electronic devices, availability of technologies, programs of education methods, career plans, attitudes toward pandemic, and the mental health of respondents. Univariate and bivariate statistical analysis was conducted to determine the association of variables. All statistical analyses using (IBM) SPSS version 22.0.ResultsMost of the respondents were female (69.4%), the mean age was 20.9 ± 2.1 years. More than half of the respondents (58.7%) reported that they have adequate skills in using digital devices. Most of them (74%) agreed that e-learning can be implemented in Indonesia. The infrastructure aspects that require attention are Internet access and the type of supporting devices. The pandemic also has an impact on the sustainability of the education program. It was found that 28.1% were experiencing financial problems, 2.1% postponed their education due to this problems. The delay of the education process was 32.6% and 47.5% delays in the clinical education phase. Around 4% student being sick, self-isolation and taking care sick family. the pandemic was found to affect students’ interests and future career plans (34%). The majority of students (52.2%) are concerned that the pandemic will limit their opportunities to become specialists. Nearly 40% of respondents expressed anxiety symptoms about a variety of issues for several days. About a third of respondents feel sad, depressed, and hopeless for a few days.ConclusionThe infrastructure and competency of its users are required for E-learning to be successful. The majority of medical students believe that e-learning can be adopted in Indonesia and that their capacity to use electronic devices is good. However, access to the internet remains a problem. On the other side, the pandemic has disrupted the education process and mental health, with fears of being infected with SARS-CoV-2, the loss of opportunities to apply for specialty training, and the potential for increased financial difficulties among medical students. Our findings can be used to assess the current educational process in medical schools and maximize e-learning as an alternative means of preparing doctors for the future.
Phytochemical screening, antioxidant activity and cytotoxicity assay from noni juice and fermented noni (Morinda citrifolia L.) Handayani, Devyana Dyah Wulandari, Devyani Diah Wulansari Bali Medical Journal, 2022 Introduction: Noni fruit is one of the plants that is often used in traditional medicine as a medicine for high blood pressure, beriberi, smooth urination, inflammation of the gallbladder, colitis, dysentery, constipation, spleen pain, liver disease, diabetes and back pain. This study aimed to analyze the content of bioactive compounds, antioxidant properties and cytotoxic activity of fermented noni juice and noni juice. Method: Identification of the content of bioactive compounds was carried out using Gas Chromatography (GC), antioxidant activity testing using the DPPH method (2,2-Diphenyl-1 picrylhydrazine) and cytotoxic testing using the Brine Shrimp Lethality Test (BSLT) method. Results: The results showed that the content of phenolic compounds contained in noni juice and fermented noni juice had strong antioxidant properties with the IC50 value for noni juice of 22.23 ppm and for fermented noni juice of 13.95 ppm. Meanwhile, the results of the cytotoxic test of noni juice and fermented noni showed that the LC50 values of 33.26 ppm and 2.25 ppm respectively. Conclusion: It can be concluded that both of these materials have high antioxidant activity and potential as anticancer.
Potential therapy of Sambiloto plant (Andrographis paniculata) using multi-compounds analysis Handayani, Wiwik Winarningsih Gaceta Medica De Caracas, 2021 Introduction: Sambiloto plant (Andrographis paniculata) is often used as herbal medicine plant in Indonesia. Previous evidence indicates the use of a whole plant or single-compound approach. Analysis of multi-compounds is needed to determine the therapeutic potential for standardizing herbal medicine to provide a reliable effect. Methods: An exploratory study searching for the active content of A. paniculata was carried out in the Knapsack program. The chemical structure is analyzed computationally using Prediction of Activity Spectra for Substances (PASS) software. The analysis of the mechanism of action of drug molecules was analyzed using the Search Tool for Interacting Chemicals (STITCH) software. Results: The active content of A. paniculata is 46 types, with 5 of them having 6 effects based on chemical bonds and targeting 12 receptor proteins. Five active contents of A. paniculata include andrographidin A, caffeic acid, chlorogenic acid, wogonin 5-glucoside, and cinnamic acid. Analysis of the mechanism of action of A. paniculata based on 12 target proteins from active ingredients using a multi-compound approach shows 6 unique biological processes. Based on the chemical bonds, 5 active contents of A. paniculata have six effects, including anaphylatoxin receptor antagonist, a beta-adrenergic receptor kinase inhibitor, GABA C receptor agonist, G-protein-coupled receptor kinase inhibitor, Aryl hydrocarbon receptor agonist, and Nicotinic alpha6beta3beta4alpha5 receptor antagonist. Conclusion: There is a therapeutic potential of A. paniculata with multi-compounds analysis. A molecular docking analysis is needed to predict the affinity between the ligand (active ingredient) and the target protein.
DOSE DEPENDENT IMUNOMODULATORY EFFECTS OF THE HERBAL COMBINATIONSAMBILOTO–GINGER–TURMERIC (SIJAKUN): AN IN VIVO STUDY ON WISTAR RAT HANDAYANI, HM SALIM, A MUSTIKA, AINDS RAMADHANA International Journal of Applied Pharmaceutics 18 (https://www.researchgate … , 2026 2026
Empowerment of Santri in Preventing Acute Respiratory Infections (ARI) through Education on Immune System Resilience and Herbal Processing BE Suwito, H Handayani, TS Agustina, TAP Setiawan, EA Maula, ... INDONESIA BERDAYA journal of Community Engagement, https://doi.org/10.47679 … , 2025 2025
Ultrasound-Based Knee Osteoarthritis Severity Assessment and Its Association With Kellgren–Lawrence Grading ANR Rita Vivera Pane, Handayani, Aisyah, Nurhasyimah Hisamud-Din, Aufar ... The Scientific World Journalhttps://aps.wiley.com/view.manuscript/tswj … , 2025 2025
Melinjo Seeds Extract Attenuates Mercury chloride Exposure-Evoked Liver Damage via Modulation Oxidative stress marker in Rats H Devyana Dyah Wulandari1*, Mardi Santoso2 , Andreas Putro Ragil Santoso1 ... Reseach Journal of Pharmacy and Technology (RJPT) 18 (1), 127-133 , 2025 2025
Short-term evaluation of hypertonic dextrose prolotherapy intra-articular injection on femoral cartilage thickness among Knee Osteoarthritis Patients with Obesity RV Pane, Aisyah, Handayani, AZZA Hajiri International Islamic Medical Journal (IIMJ) DOI : https://doi.org/10.33086 … , 2024 2024
Pelatihan Pembuatan Minuman Herbal pada KADER SIAGA KUSTA di Dusun Sumber Glagah, Kab Mojokerto RDPE Handayani, Bastiana Bastiana, Wiwik Winarningsih, Ratna Sofaria Munir ... Community Development Journal: Jurnal Pengabdian Masyarakat 5 (Universitas … , 2024 2024
In Vitro Antioxidant and Anti-Inflamatory Properties of Ethyl Acetate Extract Garcina Tetranda Pierre Fruit Peel DD Wulandari, APR Santoso, SN Cahyani, N Chilmiyah, I Erika, ... Journal of Hunan University (Natural Sciences) 51 (10), 58-67 , 2024 2024
Anti-Inflammatory Effects of the Herbal Combination Sambiloto-Ginger-Turmeric (SIJAKUN) RZ Handayani Handayani1 , Ardyarini Dyah Savitri1 , Afira Febriani Surya ... Journal of Medicinal and chemical Sciences 7 (Journal homepage: http://www … , 2024 2024
Pemanfaatan Tanaman Herbal untuk Peningkatan Daya tahan Tubuh Dalam Masa Pandemi Covid 19 di Pondok Pesantren Al Fithrah Surabaya H Handayani Journal of Dedicators Community 8 (Universitas Nahdlatul Ulama Jepara), 10-14 , 2024 2024
Pemberdayaan Kemitraan Masyarakat : Kampung Siaga Kusta di Dusun Sumber Glagah Desa Tanjung Kenongo, Kecamatan Pacet, Kabupaten Mojokerto AAG Handayani Handayani, Wiwik Winarningsih Journal of Dedicators Community 8 (Universitas Nahdlatul Ulama Jepara) , 2024 2024 Citations: 1
Oxidative DNA Damage and Metabolic Profile Disorder as Indicators of Prognosis of Active TB Patient with Anti-Tuberculosis Treatment M Hotimah, Handayani, DI Krisnawati SINTA , 2024 2024
Pemanfaatan Minuman Herbal SIJAKUN untuk Meningkatkan Imunitas pada Komunitas Imigran di PUSPA AGRO Sidoarjo H Handayani, RS Munir, RN Puspitasari, EBP Putri, BM Oktaviana, ... Community Development Journal: Jurnal Pengabdian Masyarakat, Lembaga … , 2023 2023
Edukasi PencegahanObesitas pada Remaja Santri Pondok Pesantren Hidayatullah AL MUHAJIRIN Bangkalan RV Pane, A Aisyah, H Handayani, AZZA Hajiri Community Development Journal : Jurnal Pengabdian Masyarakat 4 (6), 12744-12747 , 2023 2023
The Relationship Of Anti-Inflammation Non Steroid Use Patterns With Side Effects In Patients Post Appendectomy At JemursariIslamic Hospital SN Lailia1, Handayani1*, A Bhayusakti1, AA Adriansyah2 International Islamic Medical Journal 5 (Universitas Nahdlatul Ulama … , 2023 2023
Hepatoprotective effect of ethanolic extract of Syzygium polyanthum in rats induced by rifampicin and isoniazid DDW Handayani, Renny Novi Puspitasari, Lysa Veterini Bali Medical Journal, http://www.balimedicaljournal.org/; DOI http://dx.doi … , 2023 2023 Citations: 1
Pemberdayaan Ibu-Ibu PKK Desa Masangan Kulon Melalui Pelatihan Pembuatan Produk Halal Berbahan Dasar Susu DD WULANDARI, HANDAYANI, EBP PUTRI, VNUR PRATIWI BERNAS: Jurnal Pengabdian Kepada Masyarakat 4 (https://ejournal.unma.ac.id … , 2023 2023
Antioxidant potential effect combination of the bitter, red ginger, and turmeric extract with the DPPH method H Handayani, AD Savitri, RN Puspitasari, AZZA Hajiri, RDA Iryawan, ... Bali Medical Journal (Bali MedJ) 2022, Volume 11, Number 3: 2071-2074 P-ISSN … , 2022 2022
The Use of Antibiotics without Prescription: a Literature Review AK Dewi, Handayani, AFS W2 International Islamic Medical Journal 4 (Universitas Nahdlatul Ulama … , 2022 2022 Citations: 1
Drug Interactions in Coronary Heart Disease Patients: a Literature Review AF Elok Tamarah, Handayani H* International Islamic Medical Journal DOI : 10.33086/iimj.v4i1.3855 ; https … , 2022 2022 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
The Effect of High-Fat Diet on Histological Changes of Kidney in Rats H Hotimah Masdan Salim, Lintan Farizqi Kurnia, Tri Wahyu Bintarti Biomolecular and Health Sience Journal 2 (2), 109-112 , 2018 2018 Citations: 31
Impact on Medical Education and the Medical Student’s Attitude, Practice, Mental Health, After One Year of the Covid-19 Pandemic in Indonesia YT all Frontiers in Education 7 (https://www.frontiersin.org/), 8439-98 , 2022 2022 Citations: 25
Pencegahan stunting di Kabupaten Pasuruan dengan pelatihan pengolahan ikan gabus Handayani, W Wiwik, FK Muhammad Journal of Dedicator Community 6 (1), 59-66 , 2022 2022 Citations: 15
Effect of bay leaf (Syzygium polyanthum) extract on antioxidant activity, MDA levels, and liver histopathology feature of ethambutol induced wistar rats BE Suwito, LM Shanty, R Gumilang, H Handayani, RA Ulhaq Indonesian Journal of Medical Laboratory Science and Technology 4 (2), 148-156 , 2022 2022 Citations: 9
Apoptotic effect of Physalis minima Linn ethanol extract on breast cancer cells via p53 wild-type and Apaf-1 protein H Handajani, R Handayani, I Susilo, A basori, HM Salim Tropical Journal of Pharmaceutical Research 19 (10), 2085-2089 , 2020 2020 Citations: 9
“Perubahan Kemampuan Komunikasi Verbal Pasien Stroke Iskemik Dengan Disartria Pasca LSVT LOUD di RSI Jemursari Surabaya YK Yuliastuti, Retno Ayu, Handayani Handayani Jurnal Ilmiah Keperawatan (Scientific Journal of Nursing) 2 (https://doi.org … , 2018 2018 Citations: 8
Phytochemical screening, antioxidant activity and cytotoxicity assay from noni juice and fermented noni (Morinda citrifolia L.) H Handayani*, DD Wulandari, DD Wulansari Bali Medical Journal (Bali MedJ) 11 (2022, Volume 11, Number 3: 1168-1171 … , 2022 2022 Citations: 4
Community Service About the Risk of Degenerative Diseases and Infections in Aru Surabaya Student Association (HIMAS) Dorms R Anggraini, Handayani, ES Rofi'usyani, EJ Selly, M Malae Journal of Health and Community Service DOI: 10.33086/jhcs.v2i1.2688 2 … , 2022 2022 Citations: 3
The Effects of Turmeric Infusion and Turmeric Juice (Curcuma Domestica) on The Staphylococcus Aureus Growth in Vitro H Handayani, RN Puspitasari, R Sofaria International Islamic Medical Journal 2 (2), 54-60 , 2021 2021 Citations: 3
Edukasi Kebersihan Diri Dan Kesehatan Lingkungan Pada Program Kemitraan Masyarakat Pembentukan Poskestren Di Pondok Pesantren Al Hikam Bangkalan Madura TW Bintarti, H Handayani, RPN Budiarti Community Development Journal 3 (2), 89-94 , 2019 2019 Citations: 3
The Effect of Combination Spherical Grip and Kinesio Taping Exercise to Enhance of Muscle Strength in Ischemic Stroke Patient in Jemursari Islamic Hospital Surabaya WH Qori Mariyanto, Handayani Jurnal Ners dan Kebidanan Indonesia 6 (DOI: http://dx.doi.org/10.21927/jnki … , 2018 2018 Citations: 3
Skrining HIV pada Remaja di Surabaya dengan Menggunakan RAPID TEST MYA Thomas S, Rahayu A, Handayani Handayani Medical and Health Science Journal 1 (2) , 2017 2017 Citations: 3
Analisis Status Gizi Terhadap Tingkat Kelelahan Kerja Pada Pekerja Divisi Kapal Perang PT. PAL Indonesia (Persero) AA Adriansyah, TA Imanniyah, H Handayani Medical Technology and Public Health Journal 2 (2), 101-108 , 2018 2018 Citations: 2
Traditional to Rational and Modern Phytopharmaca H Handayani Proceeding Surabaya International Health Conference 2017 1 (1) , 2017 2017 Citations: 2
Pemberdayaan Kemitraan Masyarakat : Kampung Siaga Kusta di Dusun Sumber Glagah Desa Tanjung Kenongo, Kecamatan Pacet, Kabupaten Mojokerto AAG Handayani Handayani, Wiwik Winarningsih Journal of Dedicators Community 8 (Universitas Nahdlatul Ulama Jepara) , 2024 2024 Citations: 1
Hepatoprotective effect of ethanolic extract of Syzygium polyanthum in rats induced by rifampicin and isoniazid DDW Handayani, Renny Novi Puspitasari, Lysa Veterini Bali Medical Journal, http://www.balimedicaljournal.org/; DOI http://dx.doi … , 2023 2023 Citations: 1
The Use of Antibiotics without Prescription: a Literature Review AK Dewi, Handayani, AFS W2 International Islamic Medical Journal 4 (Universitas Nahdlatul Ulama … , 2022 2022 Citations: 1
Drug Interactions in Coronary Heart Disease Patients: a Literature Review AF Elok Tamarah, Handayani H* International Islamic Medical Journal DOI : 10.33086/iimj.v4i1.3855 ; https … , 2022 2022 Citations: 1
Edukasi Pembuatan Yogurt Susu Kambing Sebagai Upaya Peningkatan Minat Berwirausaha Pada Ibu PKK Kalidami, Mojo, Gubeng, Surabaya HH Endah Budi Permana Putri, Rizki Nurmalya Kardina, Youwan Tobing Lukiyono Wisanggeni: Jurnal Pengabdian Masyarakat 2 (Volume 2, Nomor 1, Juni 2022), 1-7 , 2022 2022 Citations: 1
Why Does de-Radicalizayion Seem A Utopia? Evaluation on "Chlidren of The Country" Program H ALGRISTIAN, HANDAYANI H Journal of Public Health in Africa 10 (1), https://www.publichealthinafrica … , 2020 2020 Citations: 1
