LESNIEWSKA
@u-bourgogne.fr
ICB/Department of Nanosciences/Faculty of Science/University of Bourgogne
University of Bourgogne
P.Vitry, E.Bourillot, L.Tétard, C.Plassard, Y.Lacroute, E.Lesniewska “Mode-Synthesizing Atomic Force Microscopy for volume characterization of mixed metal nanoparticles“, J. Microscopy, 263, 307-311, 2016.
V.Optasanu, E.Bourillot, P.Vitry, C.Plassard, L.Beaurenaut, P.Jacquinot , F.Herbst, P.Berger, E.Lesniewska, T.Montesin “High-resolution characterization of the diffusion of light chemical elements in metallic components by Scanning Microwave Microscopy“, Nanoscale, 6, 14932-14938, 2014.
H. Nasrallah, A. Vial, N. Pocholle, J. Soulier, L. Costa, C. Godefroy, E. Bourillot, E. Lesniewska, P-M. Milhiet “Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy“, Book Atomic Force Microscopy, 45-59, 2019.
R. Ionescu, R. Selon, N. Pocholle, L. Zhou, A. Rumyantseva, E. Bourillot, E. Lesniewska “Microwave Spectroscopic Detection of Human Hsp70 Protein on Annealed Gold Nanostructures on ITO Glass Strips“, Biosensors, 8(4), 118, 2018.
EDUCATION
2000: Habilitation to Direct Research HDR, Speciality Sciences Physics Mathematics (UB).
1992: PhD in Physics of the University of Bourgogne (UB).
1989: Master in Physics
RESEARCH INTERESTS
Nanophysics: nanoelectronics, nanophotonics, nanomagnetism, nanoelectromechanics, etc.
Physics of biological systems
Characterisation methods of materials
Surface science and nanostructures
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Benjamin King, Sujithkumar Ganesh Moorthy, Eric Lesniewska, Rita Meunier-Prest, Marcel Bouvet, and Benoît H. Lessard
Elsevier BV
Marie Garnier, Eric Lesniewska, Virgil Optasanu, Bruno Guelorget, Pascal Berger, Luc Lavisse, Manuel François, Irma Custovic, Nicolas Pocholle, and Eric Bourillot
MDPI AG
Conventional techniques that measure the concentration of light elements in metallic materials lack high-resolution performance due to their intrinsic limitation of sensitivity. In that context, scanning microwave microscopy has the potential to significantly enhance the quantification of element distribution due to its ability to perform a tomographic investigation of the sample. Scanning microwave microscopy associates the local electromagnetic measurement and the nanoscale resolution of an atomic force microscope. This technique allows the simultaneous characterization of oxygen concentration as well as local mechanical properties by microwave phase shift and amplitude signal, respectively. The technique was calibrated by comparison with nuclear reaction analysis and nanoindentation measurement. We demonstrated the reliability of the scanning microwave technique by studying thin oxygen-enriched layers on a Ti-6Al-4V alloy. This innovative approach opens novel possibilities for the indirect quantification of light chemical element diffusion in metallic materials. This technique is applicable to the control and optimization of industrial processes.
Abhishek Kumar, Ikechukwu David Nwosu, Rita Meunier-Prest, Eric Lesniewska, and Marcel Bouvet
American Chemical Society (ACS)
Modulation of interfacial conductivity in organic heterostructures is a highly promising strategy to improve the performance of electronic devices. In this endeavor, the present work reports the fabrication of a bilayer heterojunction device, combining octafluoro copper phthalocyanine (CuF8Pc) and lutetium bis-phthalocyanine (LuPc2) and tunes the charge transport at the Cu(F8Pc)-(LuPc2) interface by aryl electrografting on the device electrode to improve the device NH3-sensing properties. Dimethoxybenzene (DMB) and tetrafluoro benzene (TFB) electrografted by an aryldiazonium electroreduction method form a few-nanometer-thick organic film on ITO. The conductivity of the heterojunction devices formed by coating a Cu(F8Pc)/LuPc2 bilayer over the aryl-grafted electrode strongly varies according to the electronic effects of the substituents in the aryl. Accordingly, DMB increases while TFB decreases the mobile charges accumulation at the Cu(F8Pc)-(LuPc2) interface. This is explained by the perfect alignment of the frontier molecular orbitals of DMB and Cu(F8Pc), facilitating charge injection into the Cu(F8Pc) layer. On the contrary, TFB behaves like a strong acceptor and reduces the mobile charges accumulation at the Cu(F8Pc)-(LuPc2) interface. Such interfacial conductivity variation influences the device NH3-sensing properties, which increase because of DMB grafting and decrease in the presence of TFB. DMB-based heterojunction devices contain four times higher active sites for NH3 adsorption and could detect NH3 down to 1 ppm with limited interference from humidity, making them suitable for real environment NH3 detection.
Clément Nivet, Irma Custovic, Laure Avoscan, Floris J. Bikker, Aline Bonnotte, Eric Bourillot, Loïc Briand, Hélène Brignot, Jean-Marie Heydel, Noémie Herrmann,et al.
MDPI AG
The mucosal pellicle (MP) is a biological film protecting the oral mucosa. It is composed of bounded salivary proteins and transmembrane mucin MUC1 expressed by oral epithelial cells. Previous research indicates that MUC1 expression enhances the binding of the main salivary protein forming the MP, MUC5B. This study investigated the influence of MUC1 structure on MP formation. A TR146 cell line, which does not express MUC1 natively, was stably transfected with genes coding for three MUC1 isoforms differing in the structure of the two main extracellular domains: the VNTR domain, exhibiting a variable number of tandem repeats, and the SEA domain, maintaining the two bound subunits of MUC1. Semi-quantification of MUC1 using dot blot chemiluminescence showed comparable expression levels in all transfected cell lines. Semi-quantification of MUC5B by immunostaining after incubation with saliva revealed that MUC1 expression significantly increased MUC5B adsorption. Neither the VNTR domain nor the SEA domain was influenced MUC5B anchoring, suggesting the key role of the MUC1 N-terminal domain. AFM-IR nanospectroscopy revealed discernible shifts indicative of changes in the chemical properties at the cell surface due to the expression of the MUC1 isoform. Furthermore, the observed chemical shifts suggest the involvement of hydrophobic effects in the interaction between MUC1 and salivary proteins.
Benazir Khurshid, Eric Lesniewska, Luca Polacchi, Maëva L'Héronde, Daniel J. Jackson, Sébastien Motreuil, Jérôme Thomas, Jean-François Bardeau, Stephan E. Wolf, Daniel Vielzeuf,et al.
Elsevier BV
Lorena Di Zazzo, Sujithkumar Ganesh Moorthy, Rita Meunier-Prest, Eric Lesniewska, Corrado Di Natale, Roberto Paolesse, and Marcel Bouvet
MDPI AG
The versatility of metal complexes of corroles has raised interest in the use of these molecules as elements of chemical sensors. The tuning of the macrocycle properties via synthetic modification of the different components of the corrole ring, such as functional groups, the molecular skeleton, and coordinated metal, allows for the creation of a vast library of corrole-based sensors. However, the scarce conductivity of most of the aggregates of corroles limits the development of simple conductometric sensors and requires the use of optical or mass transducers that are rather more cumbersome and less prone to be integrated into microelectronics systems. To compensate for the scarce conductivity, corroles are often used to functionalize the surface of conductive materials such as graphene oxide, carbon nanotubes, or conductive polymers. Alternatively, they can be incorporated into heterojunction devices where they are interfaced with a conductive material such as a phthalocyanine. Herewith, we introduce two heterostructure sensors combining lutetium bisphthalocyanine (LuPc2) with either 5,10,15-tris(pentafluorophenyl) corrolato Cu (1) or 5,10,15-tris(4-methoxyphenyl)corrolato Cu (2). The optical spectra show that after deposition, corroles maintain their original structure. The conductivity of the devices reveals an energy barrier for interfacial charge transport for 1/LuPc2, which is a heterojunction device. On the contrary, only ohmic contacts are observed in the 2/LuPc2 device. These different electrical properties, which result from the different electron-withdrawing or -donating substituents on corrole rings, are also manifested by the opposite response with respect to ammonia (NH3), with 1/LuPc2 behaving as an n-type conductor and 2/LuPC2 behaving as a p-type conductor. Both devices are capable of detecting NH3 down to 10 ppm at room temperature. Furthermore, the sensors show high sensitivity with respect to relative humidity (RH) but with a reversible and fast response in the range of 30–60% RH.
Pattaraporn Panraksa, Pornchai Rachtanapun, Parichat Thipchai, Eric Lesniewska, Claire-Hélène Brachais, Frédéric Debeaufort, Odile Chambin, and Pensak Jantrawut
Elsevier BV
Geetika Raizada, Balasubramaniam Namasivayam, Sameh Obeid, Benjamin Brunel, Wilfrid Boireau, Eric Lesniewska, and Celine Elie-Caille
MyJove Corporation
Extracellular vesicles (EVs) are membrane-derived, tiny vesicles produced by all cells that range from 50 to several hundreds of nanometers in diameter and are used as a means of intercellular communication. They are emerging as promising diagnostic and therapeutic tools for a variety of diseases. There are two main biogenesis processes used by cells to produce EVs with differences in size, composition, and content. Due to their high complexity in size, composition, and cell origin, their characterization requires a combination of analytical techniques. This project involves the development of a new generation of multiparametric analytical platforms with increased throughput for the characterization of subpopulations of EVs. To achieve this goal, the work starts from the nanobioanalytical platform (NBA) established by the group, which allows an original investigation of EVs based on a combination of multiplexed biosensing methods with metrological and morphomechanical analyses by atomic force microscopy (AFM) of vesicular targets trapped on a microarray biochip. The objective was to complete this EV investigation with a phenotypic and molecular analysis by Raman spectroscopy. These developments enable the proposal of a multimodal and easy-to-use analytical solution for the discrimination of EV subsets in biological fluids with clinical potential.
Irma Custovic, Nicolas Pocholle, Eric Bourillot, Eric Lesniewska, and Olivier Piétrement
Springer Science and Business Media LLC
AbstractSignificant efforts have been done in last two decades to develop nanoscale spectroscopy techniques owning to their great potential for single-molecule structural detection and in addition, to resolve open questions in heterogeneous biological systems, such as protein–DNA complexes. Applying IR-AFM technique has become a powerful leverage for obtaining simultaneous absorption spectra with a nanoscale spatial resolution for studied proteins, however the AFM-IR investigation of DNA molecules on surface, as a benchmark for a nucleoprotein complexes nanocharacterization, has remained elusive. Herein, we demonstrate methodological approach for acquisition of AFM-IR mapping modalities with corresponding absorption spectra based on two different DNA deposition protocols on spermidine and Ni2+ pretreated mica surface. The nanoscale IR absorbance of distinctly formed DNA morphologies on mica are demonstrated through series of AFM-IR absorption maps with corresponding IR spectrum. Our results thus demonstrate the sensitivity of AFM-IR nanospectroscopy for a nucleic acid research with an open potential to be employed in further investigation of nucleoprotein complexes.
Navya Maryjose, Irma Custovic, Laroussi Chaabane, Eric Lesniewska, Olivier Piétrement, Odile Chambin, and Ali Assifaoui
Elsevier BV
Abhishek Kumar, Rita Meunier-Prest, Eric Lesniewska, and Marcel Bouvet
Elsevier BV
Abhishek Kumar, Rita Meunier-Prest, Frédéric Herbst, Olivier Heintz, Eric Lesniewska, and Marcel Bouvet
Elsevier BV
Abhishek Kumar, Nada Alami Mejjati, Rita Meunier-Prest, Anna Krystianiak, Olivier Heintz, Eric Lesniewska, Charles H. Devillers, and Marcel Bouvet
Elsevier BV
Fiona Zoz, Stéphane Guyot, Cosette Grandvalet, Mélanie Ragon, Eric Lesniewska, Sébastien Dupont, Olivier Firmesse, Brigitte Carpentier, and Laurent Beney
MDPI AG
Although relative air humidity (RH) strongly influences microbial survival, its use for fighting surface pathogens in the food industry has been inadequately considered. We asked whether RH control could destroy Listeria monocytogenes EGDe by envelope damage. The impact of dehydration in phosphate-buffered saline (PBS) at 75%, 68%, 43% and 11% RH on the bacterial envelope was investigated using flow cytometry and atomic force microscopy. Changes after rehydration in the protein secondary structure and peptidoglycan were investigated by infrared spectroscopy. Complementary cultivability measurements were performed by running dehydration–rehydration with combinations of NaCl (3–0.01%), distilled water, city water and PBS. The main results show that cell membrane permeability and cell envelope were greatly altered during dehydration in PBS at 68% RH followed by rapid rehydration. This damage led cells to recover only 67% of their initial volume after rehydration. Moreover, the most efficient way to destroy cells was dehydration and rehydration in city water. Our study indicates that rehydration of dried, sullied foods on surfaces may improve current cleaning procedures in the food industry.
Francis Canon, Christine Belloir, Eric Bourillot, Hélène Brignot, Loïc Briand, Gilles Feron, Eric Lesniewska, Clément Nivet, Chantal Septier, Mathieu Schwartz,et al.
American Chemical Society (ACS)
Flavor is one of the main drivers of food consumption and acceptability. It is associated with pleasure feels during eating. Flavor is a multimodal perception corresponding to the functional integration of information from the chemical senses: olfaction, gustation, and nasal and oral somatosensory inputs. As a result, astringency, as a sensation mediated by the trigeminal nerves, influences food flavor. Despite the importance of astringency in food consumer acceptance, the exact chemosensory mechanism of its detection and the nature of the receptors activated remain unknown. Herein, after reviewing the current hypotheses on the molecular origin of astringency, we proposed a ground-breaking hypothesis on the molecular mechanisms underpinning this sensation as a perspective for future research.
S Oussidhoum, D Hocine, M O Bensidhoum, D Chaumont, E Bourennane, S Boudinar, A Moussi, E Lesniewska, N Geoffroy, and M S Belakid
Springer Science and Business Media LLC
Luis F. Garcia-Alles, Katharina Root, Laurent Maveyraud, Nathalie Aubry, Eric Lesniewska, Lionel Mourey, Renato Zenobi, and Gilles Truan
Public Library of Science (PLoS)
The carboxysome is a bacterial micro-compartment (BMC) subtype that encapsulates enzymatic activities necessary for carbon fixation. Carboxysome shells are composed of a relatively complex cocktail of proteins, their precise number and identity being species dependent. Shell components can be classified in two structural families, the most abundant class associating as hexamers (BMC-H) that are supposed to be major players for regulating shell permeability. Up to recently, these proteins were proposed to associate as homo-oligomers. Genomic data, however, demonstrated the existence of paralogs coding for multiple shell subunits. Here, we studied cross-association compatibilities among BMC-H CcmK proteins of Synechocystis sp. PCC6803. Co-expression in Escherichia coli proved a consistent formation of hetero-hexamers combining CcmK1 and CcmK2 or, remarkably, CcmK3 and CcmK4 subunits. Unlike CcmK1/K2 hetero-hexamers, the stoichiometry of incorporation of CcmK3 in associations with CcmK4 was low. Cross-interactions implicating other combinations were weak, highlighting a structural segregation of the two groups that could relate to gene organization. Sequence analysis and structural models permitted to localize interactions that would favor formation of CcmK3/K4 hetero-hexamers. Attempts to crystallize these CcmK3/K4 associations conducted to the unambiguous elucidation of a CcmK4 homo-hexamer structure. Yet, subunit exchange could not be demonstrated in vitro. Biophysical measurements showed that hetero-hexamers are thermally less stable than homo-hexamers, and impeded in forming larger assemblies. These novel findings are discussed in frame with reported data to propose a functional scenario in which minor CcmK3/K4 incorporation in shells would introduce sufficient local disorder as to allow shell remodeling necessary to adapt rapidly to environmental changes.
Ece Neslihan Aybeke, Sarah Ployon, Marine Brulé, Brice De Fonseca, Eric Bourillot, Martine Morzel, Eric Lesniewska, and Francis Canon
American Chemical Society (ACS)
The mucosal pellicle, also called salivary pellicle, is a thin biological layer made of salivary and epithelial constituents, lining oral mucosae. It contributes to their protection against microbiological, chemical or mechanical insults. Pellicle formation depends on the cells' surface properties, and in turn the pellicle deeply modifies such properties. It has been reported that the expression of the transmembrane mucin MUC1 in oral epithelial cells improves the formation of the mucosal pellicle. Here, we describe an approach combining classical and functionalized tip atomic force microscopy and scanning microwave microscopy to characterize how MUC1 induces changes in buccal cells' morphology, hydrophobicity and electric properties to elucidate the physicochemical mechanisms involved in the enhancement of the anchoring of salivary proteins. We show that MUC1 expression did not modify drastically the morphology of the epithelial cells' surface. MUC1 expression, however, resulted in the presence of more hydrophobic and more charged areas at the cell surface. The presence of salivary proteins decreased the highest attractive and repulsive forces recorded between the cell surface and a functionalized hydrophobic AFM tip, suggesting that the most hydrophobic and charged areas participate in the binding of salivary proteins. The cells' dielectric properties were altered by both MUC1 expression and the presence of a mucosal pellicle. We finally show that in absence of MUC1, the pellicle appeared as a distinct layer poorly interacting with the cells' surface. This integrative AFM/SMM approach may usefully describe the surface properties of various cell types, with relevance to the bioadhesion or biomimetics fields.
S Oussidhoum, D Hocine, D Chaumont, A Crisbasan, M Bensidhoum, E Bourennane, A Moussi, E Lesniewska, N Geoffroy, and M S Belkaid
IOP Publishing
Titanium dioxide (TiO2) is very employed in solar cells due to its interesting physicochemical and optical properties allowing high device performances. Considering the extension of applications in nanotechnologies, nanocrystalline TiO2 is very promising for nanoscale components. In this work, nanocrystalline TiO2 thin films were successfully deposited on porous silicon (PSi) by metal organic chemical vapor deposition (MOCVD) technique at temperature of 550 °C for different periods of times: 5, 10 and 15 min. The objective was to optimize the physicochemical and optical properties of the TiO2/PSi films dedicated for photovoltaic application. The structural, morphological and optical properties of the elaborated TiO2/PSi samples were analyzed by means of x-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDX), atomic force microscopy (AFM), photoluminescence (PL) and UV-Visible absorption spectroscopy methods. The effect of deposition time on the microstructural properties which influences the optical characteristics of the obtained samples was also examined. The XRD analysis confirms the nanocrystalline structure of the deposited TiO2 composed only by anatase phase. The SEM characterization evidenced an increase in the TiO2 film thickness showing more uniform surfaces as the deposition time rises. Correspondingly, the surface roughness increases with the particle size and film thickness as indicated by AFM studies. The TiO2/PSi/Si sandwich structure evidenced by cross-sectional SEM confirms the good adherence of the TiO2 nanocrystalline film on the porous silicon forming with silicon a composite material. The UV-Vis measurements showed a considerable enhancement in optical absorption of porous silicon after the deposition of TiO2 films. Indeed, the TiO2 coatings deposited on PSi for 15 min with thickness of 200 nm have the best structure quality and exhibit, consequently, the highest absorption. From these interesting results, we demonstrate the viability of the use of the MOCVD as reproducible process for the elaboration of high-quality TiO2/PSi films.
Hussein Nasrallah, Anthony Vial, Nicolas Pocholle, Jérémy Soulier, Luca Costa, Cédric Godefroy, Eric Bourillot, Eric Lesniewska, and Pierre-Emmanuel Milhiet
Springer New York
Rodica Ionescu, Raphael Selon, Nicolas Pocholle, Lan Zhou, Anna Rumyantseva, Eric Bourillot, and Eric Lesniewska
MDPI AG
Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm2 of Hsp70.
Sarah Ployon, Martine Morzel, Christine Belloir, Aline Bonnotte, Eric Bourillot, Loïc Briand, Eric Lesniewska, Jeannine Lherminier, Ece Aybeke, and Francis Canon
Elsevier BV
Yves Jacquot, Dany Spaggiari, Julie Schappler, Eric Lesniewska, Serge Rudaz, and Guy Leclercq
Elsevier BV
V. Optasanu, E. Bourillot, R. Selon, L. Lavisse, A. Sanchot, P. Vitry, A. Kanjer, P. Berger, E. Lesniewska, and T. Montesin
Springer Science and Business Media LLC
Luis F. Garcia-Alles, Eric Lesniewska, Katharina Root, Nathalie Aubry, Nicolas Pocholle, Carlos I. Mendoza, Eric Bourillot, Konstantin Barylyuk, Denis Pompon, Renato Zenobi,et al.
Public Library of Science (PLoS)
CcmK proteins are major constituents of icosahedral shells of β-carboxysomes, a bacterial microcompartment that plays a key role for CO2 fixation in nature. Supported by the characterization of bidimensional (2D) layers of packed CcmK hexamers in crystal and electron microscopy structures, CcmK are assumed to be the major components of icosahedral flat facets. Here, we reassessed the validity of this model by studying CcmK isoforms from Synechocystis sp. PCC6803. Native mass spectrometry studies confirmed that CcmK are hexamers in solution. Interestingly, potential pre-assembled intermediates were also detected with CcmK2. Atomic-force microscopy (AFM) imaging under quasi-physiological conditions confirmed the formation of canonical flat sheets with CcmK4. Conversely, CcmK2 formed both canonical and striped-patterned patches, while CcmK1 assembled into remarkable supra-hexameric curved honeycomb-like mosaics. Mutational studies ascribed the propensity of CcmK1 to form round assemblies to a combination of two features shared by at least one CcmK isoform in most β-cyanobacteria: a displacement of an α helical portion towards the hexamer edge, where a potential phosphate binding funnel forms between packed hexamers, and the presence of a short C-terminal extension in CcmK1. All-atom molecular dynamics supported a contribution of phosphate molecules sandwiched between hexamers to bend CcmK1 assemblies. Formation of supra-hexameric curved structures could be reproduced in coarse-grained simulations, provided that adhesion forces to the support were weak. Apart from uncovering unprecedented CcmK self-assembly features, our data suggest the possibility that transitions between curved and flat assemblies, following cargo maturation, could be important for the biogenesis of β-carboxysomes, possibly also of other BMC.