Amreen Khan
@iitb.ac.in
PhD student
Indian Institute of Technology Bombay
@iitb.ac.in
PhD student
Indian Institute of Technology Bombay
Potential approaches to enhance efficacy and reduce toxicity still remain unassured in many drug delivery systems especially in gene based. This has led to widen the pool of understanding extending insight towards the scope of modification and carrier interaction with acting moiety. CRISPR-Cas technology as gene editing tool has opened access to moderate and better control regulating central dogma of cell biology. Recent developments have shown major application in elucidating personalized genomic information, manipulation of gene and as emerging machinery to cure diseases by transforming therapies treating complex gene related disorders. However, trend has seen the need to deliver the active Cre recombinase successfully to targeted cell for achieving better genomic modification. We aim at revealing the immunomodulated nanobiomaterial plateform to cascade CRISPR-Cas improving effectiveness in targeted therapy.
Drug delivery systems have been drastically researched worldwide. Likewise, many novel drug delivery systems are being explored as technology is advancing. The study of localized stimuli-responsive drug delivery systems with nanoparticles tends to serve the purpose of theranostics administration in a single tool. To minimize the unwanted effect of damaging normal tissues, especially in the case of light-responsive theranostic, microneedle presents a promising mode for delivery of high-photon activated nanoparticles to the targeted tumor site. Incorporating the benefit of chemotherapy, we have designed an effective chemo-photo activated nanoparticle combined delivery system by microneedle. Surpassing the blood-brain barrier, opsonization, protein corona and external pathogens that block the path of cargo during oral or systemic (i.v.) delivery, we have strategized a novel method of localized delivery of theranostics.
Bioinspired sustained scaffolds involve well-defined morphological cues designed with unique physicochemical properties for a said medical application. The variations can be seen during the optimizing steps of sponge preparation. Here in this work, we have analyzed the effect of ion-based cross-linker calcium chloride added to polyvinyl alcohol/sodium alginate sponges containing natural origin green tea extract before and after the freeze-drying process. One set of PVA/sodium alginate/G.T. mixture was subjected to ionic concentrations of calcium ions and lyophilized. In another design, lyophilized PVA/sodium alginate/G.T. sponges were cross-linked with calcium chloride followed which was freeze-dried. The studies were conducted to assess the effect on morphology through SEM, mechanical strength, swelling and degradation rate at pH 7.4, and functional group analysis by FTIR. The release profile of green tea from the polymeric matrix performed in phosphate buffer at 37⁰C.
