K Nagalakshmi
@dgvaishnavcollege.edu.in
Assistant Professor
Scopus Publications
Scopus Publications
K. Nagalakshmi, R Monica Angeline, and G. Sriram Prasath
A and V Publications
Pimenta dioica (L.) Merr (also known as Pimenta Officinalis) belongs to the family Myrtaceae is globally as valuable spices. This is commonly known as allspice, Jamaica pepper and Pimento. This spice possesses the characteristic flavor and aroma of clove, nutmeg, cinnamon and black pepper, all combined in this one spice, hence named allspice. Allspice is used for treating indigestion which might be due to the abundance of the common polyphenol Eugenol , known to stimulate digestive enzymes. The present study was aimed in analysing the bioactive compounds present in P.diocia. Additionally, the antibacterial efficacy of P.diocia was determined. GC-MS Pattern of the ethanolic extract confirms the presence of eugenol a potent bioactive compound and qualitative analysis revealed the presence of various phytoconstituents. The ethanolic extract of plant proved to have anti-microbial effect on disease causing microbes such as Pseudomonas aeruginosa ,Bacillus anthracis, Klebsiella pneumoniae and Streptococcus pneumoniae with MIC of 5mg of the ethanolic extract which may be due to the different phytocontituents in the plant extract and partly due to eugenol a potent anti-microbial compound. The results of the present study indicate that Pimenta dioica is a good source of bioactive compounds and possess antibacterial activity which might be due to presence of eugenol.
NAGALAKSHMI K., SHILA S., INBATHAMIZH L., THENMOZHI A., RASAPPAN P., and SRINIVASAN P. T.
Innovare Academic Sciences Pvt Ltd
Objective: The objective of the study was to analyse the target-ligand interactions between nuclear factor-κB (NF-κB) and chelated Zinc compounds and to explore the anticancer drug potential of these ligands by a bio computational approach.
 Methods: Bioinformatics databases and tools were applied for the study. Three dimensional structure of the target NF-κB was retrieved from Protein Data Bank (PDB). The optimized structures of two chelated Zinc compounds, Zinc acetate and Zinc orotate were taken for docking studies with the target using docking tool AutoDock 4.2. Drug properties of the ligands were further assessed by Molinspiration server.
 Results: Docking results as predicted by AutoDock and as visualized by PyMol viewer were effective for both the ligands. Comparatively, Zinc orotate showed minimum energy and more interactions with the target. Both the ligands satisfied the Lipinski’s rule of five with zero violations.
 Conclusion: The findings emphasized the promising role of chelated Zinc compounds as potent drug candidates in anti-cancer drug design against NF-κB.
Nagalakshmi K and Sujatha S.
Innovare Academic Sciences Pvt Ltd
Objective: This study was designed to study the release efficacy and glucose tolerance of 14-deoxy, 11, 12-didehydroandrographolide loaded polycaprolactone nanoparticles in streptozotocin-nicotinamide induced type 2 diabetes.Methods: Biodegradable polymer based novel drug delivery systems had brought a considerable attention to improve therapeutic efficacy and bioavailability of various drugs. In this study, 14-deoxy-11, 12-didehydroandrographolide (sparingly water soluble) loaded polycaprolactone (nano-DDA) was synthesized using polyvinyl alcohol and tween20 as surfactants. MTT assay was performed to analyse the cytotoxicity of both the formulations on L6 myoblasts. Free DDA and nano-DDA were administered orally to the streptozotocin-nicotinamide induced experimental diabetic rats for 45d. Oral glucose tolerance test (OGTT) was carried out at the end of the study. After one week washout period, animals were administered with free and nano-DDA and release efficacy of DDA from polymer matrix and concentration of glucose were analysed.Results: MTT assay revealed that nano-DDA prepared using tween-20 as a surfactant elicited cytotoxicity towards L6 myoblasts, whereas nano-DDA prepared using polyvinyl alcohol as a surfactant remained non-toxic till 10µM. OGTT studies revealed an initial increase of glucose at 30 min followed by a progressive decrease in the glucose level. In rat plasma, a gradual decrease in glucose level was observed up to 32h (139 mg/dl) for free DDA, whereas nano-DDA exhibited a major decrease in glucose concentration at 32h (115 mg/dl) which continued even after 48h (117 mg/dl).Conclusion: A slow and sustained release of DDA from the polymer matrix substantiated that nanoencapsulation enhanced the oral bioavailability of DDA which resulted in decreasing the concentration of glucose which could be due to the pronounced antihyperglycemic activity of nano-DDA over free DDA.
US Mahadeva Rao, A Thenmozhi, and K Nagalakshmi
BRNSS Publication Hub