Silvana de Almeida
@ufcspa.edu.br
Departamento de Ciências Básicas da Saúde
Universidade Federal de Ciências Básicas da Saúde
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Joana Fisch, Ana Carolina de Moura, Vanessa Feistauer, Luiza Steffens Reinhardt, Patrícia Molz, Ana Moira Morás, Dinara Jaqueline Moura, Priscila Oliveira de Souza, Elizandra Braganhol, Silvana Almeida,et al.
Springer Science and Business Media LLC
Tharcila Quadros de Oliveira, Ana Carolina de Moura, Vanessa Feistauer, Roberto Damiani, Matheus Filipe Braga, Silvana Almeida, Renata Padilha Guedes, and Márcia Giovenardi
Elsevier BV
Mariana Fraga Gauthier, Andressa Alves de Andrade, Joana Fisch, Vanessa Feistauer, Ana Moira Morás, Luiza Steffens Reinhardt, Ana Carolina de Moura, Dinara Jaqueline Moura, Silvana de Almeida, Renata Padilha Guedes,et al.
Springer Science and Business Media LLC
Scheila da Silva Soares, Josiane Rodrigues Aquino, Francini Petrolli, Tiago Bittencourt de Oliveira, Silvana Almeida, and Marilu Fiegenbaum
Elsevier BV
Janaína Kehl de Castilhos, Paula Dal Bó Campagnolo, Silvana de Almeida, Márcia Regina Vitolo, and Vanessa Suñé Mattevi
FapUNIFESP (SciELO)
Abstract Epigenetic modifications established during prenatal and early life, including DNA methylation, have been suggested as potential mediators of the interaction between environmental exposures during the perinatal period and adult metabolic health adverse outcomes, especially cardiometabolic complications and overweight. The effect of a dietary intervention in the first year of life on global methylation levels in leukocyte samples from a cohort of children born between 2001 and 2002 in southern Brazil was examined. Overall methylation measurements were performed using enzyme-linked immunosorbent assays on DNA samples from 237 children at 4 years old. Mean methylation values were higher in the intervention group (mean: 2.20 ± 1.31%) than in the control group (mean: 1.65 ± 1.11%; P = 0.001). It was observed that nutritional counseling in the first year increased breastfeeding duration and stimulated the development of healthier eating habits. Therefore, these factors might have contributed to increase global DNA methylation. The findings of the present study reinforce the notion that performing nutritional interventions in the early stages of life is important and provide further evidence of the interaction between the environment and epigenetic traits.
Mirelen Moura de Oliveira Rodrigues, Gabriela Höher, Gabriela Waskow, Mara Helena Hutz, Juliana Dal-Ri Lindenau, Maria Luiza Petzl-Erler, Sidia Maria Callegari-Jacques, Silvana Almeida, and Marilu Fiegenbaum
FapUNIFESP (SciELO)
Abstract The study presents comparisons between blood group frequencies beyond ABO and Rh blood systems in Native American populations and previously published data from Brazilian blood donors. The frequencies of Diego (c.2561C>T, rs2285644), Kell (c.578C>T, rs8176058), Duffy (c.125A>G, rs12075, c.1−67T>C, rs2814778) and Kidd (c.838A>G, rs1058396) variants in Kaingang (n=72) and Guarani (n=234) populations from Brazil (1990-2000) were obtained and compared with data from these populations sampled during the 1960s and with individuals of different Brazilian regions. Data showed high frequencies of DI*01 and FY*01 alleles: 11.8% and 57.6% in Kaingang and 6.8% and 75.7% in Guarani groups, respectively. The main results indicated: (1) reduction in genetic distance over time of Kaingang and Guarani in relation to other Brazilian populations is suggestive of ongoing admixture; (2) significant differences in some frequencies of blood group markers (especially Diego, Kidd and Duffy) in relation to Native Americans and individuals from different geographical regions of Brazil. Our study shows that the frequency of red blood cell polymorphisms in two Native American groups is very different from that of blood donors, when we evaluated blood groups different from ABO and Rh systems, suggesting that a better ethnic characterization of blood unit receptors is necessary.
Andressa de Freitas Alves, Ana Carolina de Moura, Huander Felipe Andreolla, Ana Beatriz Gorini da Veiga, Marilu Fiegenbaum, Márcia Giovenardi, and Silvana Almeida
FapUNIFESP (SciELO)
Abstract Any condition leading to chronic liver disease is a potential oncogenic agent for hepatocellular carcinoma (HCC). Alterations in the expression of antioxidant enzymes could alter the redox balance. Our aim was to evaluate the expression of the genes GPX1, GPX4, SEP15, SELENOP, SOD1, SOD2, GSR, CAT, and NFE2L2 in patients with HCC. Differential gene expression analysis was performed using RNA-Seq data from the TCGA and GTEx databases, and RT-qPCR data from HCC patient samples. Bioinformatic analysis revealed significant differential expression in most genes. GPX4 expression was significantly increased (p=0.02), while SOD2 expression was significantly decreased (p=0.04) in experimental data. In TCGA samples, alpha-fetoprotein levels (mg/dL) were negatively correlated with the expression of SEP15 (p<0.001), SELENOP (p<0.001), SOD1 (p<0.001), SOD2 (p<0.001), CAT (p<0.001), and NFE2L2 (p=0.004). Alpha-fetoprotein levels were positively correlated with the expression of GPX4 (p=0.02) and SELENOP (p=0.01) in the experimental data. Low expression of GPX1 (p=0.006), GPX4 (p=0.01), SELENOP (p=0.006), SOD1 (p=0.007), CAT (p<0.001), and NFE2L2 (p<0.001), and higher levels of GSR, were associated with low overall survival at 12 months. These results suggest a significant role for these antioxidant enzymes in HCC pathogenesis and severity.
Vanessa Feistauer, Joana Fisch, Carolina Kalkmann da Silva Oliveira, Márcia Giovenardi, and Silvana Almeida
Elsevier BV
Gabriela Höher, Mirelen Moura de Oliveira Rodrigues, Gabriela Waskow, Grasiela Agnes, Pâmela Victoria Von Burg, Tor Onsten, Marilu Fiegenbaum, and Silvana Almeida
Elsevier BV
Gabriela Waskow, Mirelen Moura de Oliveira Rodrigues, Gabriela Höher, Tor Onsten, Juliana Dal-Ri Lindenau, Marilu Fiegenbaum, and Silvana Almeida
FapUNIFESP (SciELO)
Abstract We evaluated genetic variability among the blood groups Kell (c.578C > T and c.1790T > C), Kidd (c.838A > G), Duffy (c.125A > G, c.265C > T and c.1-67T > C), Diego (c.2561C > T), MNS (c.143T > C) and Rh (c.676G > C) in Rio Grande do Sul in southern Brazil. Genetic profiling from 382 volunteer blood donors was performed through allelic discrimination assays using a hydrolysis probe (TaqMan®) with a real-time PCR system. The sample was divided into two groups: Euro-Brazilian and Afro-Brazilian. A comparison with studies from other regions of Brazil and the 1000 Genomes Database showed significant differences for almost all polymorphisms evaluated in our population. Population differentiation between the Euro- and Afro-Brazilian groups was low (F ST value 0.055). However, when each locus was evaluated individually, KEL*06 and FY*02N.01 allele frequencies were significantly higher in the Afro-Brazilian group than in the Euro-Brazilian group. Ethnic classification that uses phenotypic criteria to find blood units with rare antigens may be important when there is a need to detect blood units with an absence of Duffy antigens. There is also a greater probability of finding donors in the Afro-Brazilian group. Taken together, the data indicate strong European and African contributions to the gene pool, with intense admixture.
Virginia Meneghini Lazzari, Josi Maria Zimmermann-Peruzatto, Grasiela Agnes, Roberta Oriques Becker, Ana Carolina de Moura, Silvana Almeida, Renata Padilha Guedes, and Marcia Giovenardi
Elsevier BV
Joana Fisch, Vanessa Feistauer, Ana Carolina de Moura, Andrew Oliveira Silva, Vanessa Bollis, Marilene Porawski, Silvana Almeida, Renata Padilha Guedes, Alethea Gatto Barschak, and Márcia Giovenardi
Elsevier BV
Vanessa Feistauer, Márcia R. Vitolo, Paula D.B. Campagnolo, Vanessa S. Mattevi, and Silvana Almeida
FapUNIFESP (SciELO)
Abstract The reward sensation after food intake may be different between individuals and variants in genes related to the dopaminergic system may indicate a different response in people exposed to the same environmental factors. This study investigated the association of TaqIA (rs1800497) and -141C InsDel (rs1799732) variants in DRD2/ANKK1 gene with food intake and adiposity parameters in a cohort of children. The sample consisted of 270 children followed until 7 to 8 years old. DNA was extracted from blood and polymorphisms were detected by PCR-RFLP analysis. Food intake and nutritional status were compared among individuals with different SNP genotypes. Children carrying the A1 allele (TaqIA) had higher energy of lipid dense foods (LDF) when compared with A2/A2 homozygous children at 7 to 8 years old (GLM p=0.004; Mann Whitney p=0.005). No association was detected with -141C Ins/Del polymorphism. To our knowledge, this is the first association study of the DRD2 TaqIA and -141C Ins/Del polymorphism with food intake and anthropometric parameters in children. DRD2 TaqIA polymorphism has been associated with a reduction in D2 dopamine receptor availability. Therefore, the differences observed in LDF intake in our sample may occur as an effort to compensate the hypodopaminergic functioning.
G. Höher, M. Fiegenbaum and S. Almeida
ACKR1, located on chromosome 1q23.2, is the gene that encodes a glycoprotein expressing the Duffy blood group antigens. This gene is transcribed in two mRNA variants yielding two isoforms, encoding proteins with 338 and 336 amino acids. This review provides a general overview of the Duffy blood group to characterise and elucidate the genetic basis of this system. The Fya and Fyb antigens are encoded by co-dominant FY*A (FY*01) and FY*B (FY*02) alleles, which differ by c.125G>A (rs12075), defining the Fy(a+b-), Fy(a-b+) and Fy(a+b+) phenotypes. The Fy(a-b-) phenotype that occurs in Africans provides an explanation for the apparent absence of Plasmodium vivax in this region: this phenotype arises from homozygosity for the FY*B allele carrying a point mutation c.1-67T>C (rs2814778), which prevents Fyb antigen expression only in red blood cells. The same mutation has also been found on the FY*A allele, but it is very rare. The Fy(a-b-) phenotype in Europeans and Asians arises from mutations in the coding region of the FY*A or FY*B allele, preventing Duffy antigen expression on any cell in the body and thus are true Duffy null phenotypes. According to the International Society for Blood Transfusion, ten alleles are associated with the null expression of the Fy antigens. Furthermore, different allelic forms of FY*B modify Fyb antigen expression, which may result in very weak or equivocal serology results. The mostly common found variants, c.265C>T (rs34599082) and c.298G>A (rs13962) -previously defined in combination only with the FY*B allele - have already been observed in the FY*A allele. Thus, six alleles have been recognised and associated with weak expression of the Fy antigens. Considering the importance of the Duffy blood group system in clinical medicine, additional studies via molecular biology approaches must be performed to resolve and clarify the discrepant results that are present in the erythrocyte phenotyping.
Josi Maria Zimmermann-Peruzatto, Virgínia Meneghini Lazzari, Grasiela Agnes, Roberta Oriques Becker, Ana Carolina de Moura, Renata Padilha Guedes, Aldo Bolten Lucion, Silvana Almeida, and Márcia Giovenardi
Springer Science and Business Media LLC
Raquel C.K. Miranda, Júlia P. Genro, Paula D.B. Campagnolo, Vanessa S. Mattevi, Márcia R. Vitolo, and Silvana Almeida
Elsevier BV
M. R. Zandoná, C. N. Sangalli, P. d. B. Campagnolo, M. R. Vitolo, S. Almeida, and V. S. Mattevi
Wiley
The prevalence of childhood obesity has been dramatically increasing in developing countries as it has been reported for developed nations. Identifying susceptibility genes in early life could provide the foundations for interventions in lifestyle to prevent obese children to become obese adults.
Silvia V. Melo, Grasiela Agnes, Márcia R. Vitolo, Vanessa S. Mattevi, Paula D.B. Campagnolo, and Silvana Almeida
FapUNIFESP (SciELO)
Abstract Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years old. The nutritional status and food intake data of 312 children were collected at three developmental stages (1, 3.9 and 7.7 years old). DNA was extracted from blood samples and the polymorphisms were analyzed by real-time polymerase chain reactions (qPCR) using hydrolysis probes as the detection method. Food intake and nutritional status were compared among individuals with different single nucleotide polymorphism (SNP) genotypes. At 3.9 years old, children homozygous (Val/Val) for the TAS1R2 Ile191Val polymorphism ingested less sugar and sugar-dense foods than children who were *Ile carriers. This finding demonstrated that a genetic variant of the T1R2 taste receptor is associated with the intake of different amounts of high sugar-content foods in childhood. This association may provide new perspectives for studying dietary patterns and nutritional status in childhood.
L Smiderle, M Fiegenbaum, M H Hutz, C R Van Der Sand, L C Van Der Sand, M E W Ferreira, R C Pires, and S Almeida
Springer Science and Business Media LLC
V.D. Baldissera, A.F. Alves, S. Almeida, M. Porawski, and M. Giovenardi
Elsevier BV
Crisciele Fontana, Márcia R. Vitolo, Paula D.B. Campagnolo, Vanessa S. Mattevi, Júlia P. Genro, and Silvana Almeida
Elsevier BV
Raquel C.K. Miranda, Silvia B. Vetter, Júlia P. Genro, Paula D.B. Campagnolo, Vanessa S. Mattevi, Márcia R. Vitolo, and Silvana Almeida
Elsevier BV
Muriel Primon-Barros, Graziela Vargas Rigo, Amanda Piccoli Frasson, Odelta dos Santos, Lisiane Smiderle, Silvana Almeida, Alexandre José Macedo, and Tiana Tasca
FapUNIFESP (SciELO)
Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.
Ana Carolina de Moura, Virgínia Meneghini Lazzari, Roberta Oriques Becker, Mirela Severo Gil, Carina Anicet Ruthschilling, Grasiela Agnes, Silvana Almeida, Ana Beatriz Gorini da Veiga, Aldo Bolten Lucion, and Márcia Giovenardi
Elsevier BV
Jéssica Aguiar de Souza, Angelica Menin, Luciana Otero Lima, Lisiane Smiderle, Mara Helena Hutz, Cézar Roberto Van Der Sand, Luiz Carlos Van Der Sand, Maria Elvira Wagner Ferreira, Renan Canibal Pires, Silvana Almeida,et al.
Elsevier BV