Impact of maternal dietary counseling in the first year of life on dna methylation in a cohort of children Janaína Kehl de Castilhos, Paula Dal Bó Campagnolo, Silvana de Almeida, Márcia Regina Vitolo, Vanessa Suñé Mattevi Genetics and Molecular Biology, 2021 Epigenetic modifications established during prenatal and early life, including DNA methylation, have been suggested as potential mediators of the interaction between environmental exposures during the perinatal period and adult metabolic health adverse outcomes, especially cardiometabolic complications and overweight. The effect of a dietary intervention in the first year of life on global methylation levels in leukocyte samples from a cohort of children born between 2001 and 2002 in southern Brazil was examined. Overall methylation measurements were performed using enzyme-linked immunosorbent assays on DNA samples from 237 children at 4 years old. Mean methylation values were higher in the intervention group (mean: 2.20 ± 1.31%) than in the control group (mean: 1.65 ± 1.11%; P = 0.001). It was observed that nutritional counseling in the first year increased breastfeeding duration and stimulated the development of healthier eating habits. Therefore, these factors might have contributed to increase global DNA methylation. The findings of the present study reinforce the notion that performing nutritional interventions in the early stages of life is important and provide further evidence of the interaction between the environment and epigenetic traits.
Gene expression evaluation of antioxidant enzymes in patients with hepatocellular carcinoma: Rt-qpcr and bioinformatic analyses Andressa de Freitas Alves, Ana Carolina de Moura, Huander Felipe Andreolla, Ana Beatriz Gorini da Veiga, Marilu Fiegenbaum, et al. Genetics and Molecular Biology, 2021 Any condition leading to chronic liver disease is a potential oncogenic agent for hepatocellular carcinoma (HCC). Alterations in the expression of antioxidant enzymes could alter the redox balance. Our aim was to evaluate the expression of the genes GPX1, GPX4, SEP15, SELENOP, SOD1, SOD2, GSR, CAT, and NFE2L2 in patients with HCC. Differential gene expression analysis was performed using RNA-Seq data from the TCGA and GTEx databases, and RT-qPCR data from HCC patient samples. Bioinformatic analysis revealed significant differential expression in most genes. GPX4 expression was significantly increased (p=0.02), while SOD2 expression was significantly decreased (p=0.04) in experimental data. In TCGA samples, alpha-fetoprotein levels (mg/dL) were negatively correlated with the expression of SEP15 (p<0.001), SELENOP (p<0.001), SOD1 (p<0.001), SOD2 (p<0.001), CAT (p<0.001), and NFE2L2 (p=0.004). Alpha-fetoprotein levels were positively correlated with the expression of GPX4 (p=0.02) and SELENOP (p=0.01) in the experimental data. Low expression of GPX1 (p=0.006), GPX4 (p=0.01), SELENOP (p=0.006), SOD1 (p=0.007), CAT (p<0.001), and NFE2L2 (p<0.001), and higher levels of GSR, were associated with low overall survival at 12 months. These results suggest a significant role for these antioxidant enzymes in HCC pathogenesis and severity.
Blood groups in native americans: A look beyond abo and rh Mirelen Moura de Oliveira Rodrigues, Gabriela Höher, Gabriela Waskow, Mara Helena Hutz, Juliana Dal-Ri Lindenau, et al. Genetics and Molecular Biology, 2021 The study presents comparisons between blood group frequencies beyond ABO and Rh blood systems in Native American populations and previously published data from Brazilian blood donors. The frequencies of Diego (c.2561C>T, rs2285644), Kell (c.578C>T, rs8176058), Duffy (c.125A>G, rs12075, c.1−67T>C, rs2814778) and Kidd (c.838A>G, rs1058396) variants in Kaingang (n=72) and Guarani (n=234) populations from Brazil (1990-2000) were obtained and compared with data from these populations sampled during the 1960s and with individuals of different Brazilian regions. Data showed high frequencies of DI*01 and FY*01 alleles: 11.8% and 57.6% in Kaingang and 6.8% and 75.7% in Guarani groups, respectively. The main results indicated: (1) reduction in genetic distance over time of Kaingang and Guarani in relation to other Brazilian populations is suggestive of ongoing admixture; (2) significant differences in some frequencies of blood group markers (especially Diego, Kidd and Duffy) in relation to Native Americans and individuals from different geographical regions of Brazil. Our study shows that the frequency of red blood cell polymorphisms in two Native American groups is very different from that of blood donors, when we evaluated blood groups different from ABO and Rh systems, suggesting that a better ethnic characterization of blood unit receptors is necessary.
Frequencies of genetic variants of the Rh, Kell, Duffy, Kidd, MNS and Diego systems of northwest Rio Grande do Sul, Brazil S da Silva Soares, JR Aquino, F Petrolli, TB de Oliveira, S Almeida, ... Hematology, Transfusion and Cell Therapy , 2022 2022 Citations: 13
Caloric restriction in mice improves short-term recognition memory and modifies the neuroinflammatory response in the hippocampus of male adult offspring TQ de Oliveira, AC de Moura, V Feistauer, R Damiani, MF Braga, ... Behavioural Brain Research 425, 113838 , 2022 2022 Citations: 6
Impact of maternal dietary counseling in the first year of life on DNA methylation in a cohort of children JK Castilhos, PDB Campagnolo, S Almeida, MR Vitolo, VS Mattevi Genetics and Molecular Biology 44 , 2021 2021 Citations: 3
Blood groups in Native Americans: a look beyond ABO and Rh MMO Rodrigues, G Höher, G Waskow, MH Hutz, JDR Lindenau, ... Genetics and Molecular Biology 44 , 2021 2021 Citations: 8
Gene expression evaluation of antioxidant enzymes in patients with hepatocellular carcinoma: RT-qPCR and bioinformatic analyses AF Alves, AC Moura, HF Andreolla, ABG Veiga, M Fiegenbaum, ... Genetics and Molecular Biology 44 , 2021 2021 Citations: 18
Restriction and hyperlipidic diets during pregnancy, lactation and adult life modified the expression of dopaminergic system related genes both in female mice and their adult … V Feistauer, J Fisch, CK da Silva Oliveira, M Giovenardi, S Almeida Brain Research Bulletin 162, 245-252 , 2020 2020 Citations: 7
Identification of ACKR1 variants associated with altered Duffy phenotype expression in blood donors from southern Brazil G Höher, MM de Oliveira Rodrigues, G Waskow, G Agnes, PV Von Burg, ... Transfusion and Apheresis Science 59 (4), 102768 , 2020 2020 Citations: 3
Genetic variability of blood groups in southern Brazil G Waskow, MMO Rodrigues, G Höher, T Onsten, JDR Lindenau, ... Genetics and Molecular Biology 43 , 2020 2020 Citations: 5
Maternal feeding associated to post-weaning diet affects metabolic and behavioral parameters in female offspring J Fisch, V Feistauer, AC de Moura, AO Silva, V Bollis, M Porawski, ... Physiology & behavior 204, 162-167 , 2019 2019 Citations: 25
Evaluation of association of DRD2 TaqIA and-141C InsDel polymorphisms with food intake and anthropometric data in children at the first stages of development V Feistauer, MR Vitolo, PDB Campagnolo, VS Mattevi, S Almeida Genetics and Molecular Biology 41 (3), 562-569 , 2018 2018 Citations: 14
Molecular basis of the Duffy blood group system G Höher, M Fiegenbaum, S Almeida Blood Transfusion 16 (1), 93 , 2018 2018 Citations: 119
Hippocampal gene expression patterns in oxytocin male knockout mice are related to impaired social interaction VM Lazzari, JM Zimmermann-Peruzatto, G Agnes, RO Becker, ... Behavioural brain research , 2017 2017 Citations: 14
The impact of oxytocin gene knockout on sexual behavior and gene expression related to neuroendocrine systems in the brain of female mice JM Zimmermann-Peruzatto, VM Lazzari, G Agnes, RO Becker, ... Cellular and molecular neurobiology 37 (5), 803-815 , 2017 2017 Citations: 27
Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children SV Melo, G Agnes, MR Vitolo, VS Mattevi, PDB Campagnolo, S Almeida Genetics and molecular biology 40 (2), 415-420 , 2017 2017 Citations: 45
Biallelic and triallelic approaches of 5-HTTLPR polymorphism are associated with food intake and nutritional status in childhood RCK Miranda, JP Genro, PDB Campagnolo, VS Mattevi, MR Vitolo, ... The Journal of Nutritional Biochemistry 43, 47-52 , 2017 2017 Citations: 12
Validation of obesity susceptibility loci identified by genome‐wide association studies in early childhood in South Brazilian children MR Zandoná, CN Sangalli, PB Campagnolo, MR Vitolo, S Almeida, ... Pediatric obesity 12 (1), 85-92 , 2017 2017 Citations: 31
ESR1 polymorphisms and statin therapy: a sex-specific approach L Smiderle, M Fiegenbaum, MH Hutz, CR Van Der Sand, ... The pharmacogenomics journal 16 (6), 507-513 , 2016 2016 Citations: 15
Differential plasma concentrations of Microrna-16-5p and Microrna-19b in Brazilian lean and obese children MR Zandoná, AS Gasparotto, PS Leffa, MR Vitolo, S Almeida, VS Mattevi Obesity Reviews 17, 13 , 2016 2016
Hepatocellular carcinoma and estrogen receptors: Polymorphisms and isoforms relations and implications VD Baldissera, AF Alves, S Almeida, M Porawski, M Giovenardi Medical hypotheses 86, 67-70 , 2016 2016 Citations: 35
Classificação de fitzpatrick: perfil fenotípico em doadores de sangue do HCPA-RS SB Leite, SC Wagner, MMO Rodrigues, S Almeida, G Waskow, ... Congresso Brasileiro de Biomedicina (15.: 2016: Bento Gonçalves, RS). Anais … , 2016 2016
MOST CITED SCHOLAR PUBLICATIONS
The TaqI A1 allele of the dopamine D2 receptor gene and alcoholism in Brazil: association and interaction with stress and harm avoidance on severity prediction CHD Bau, S Almeida, MH Hutz American journal of medical genetics 96 (3), 302-306 , 2000 2000 Citations: 140
Molecular basis of the Duffy blood group system G Höher, M Fiegenbaum, S Almeida Blood Transfusion 16 (1), 93 , 2018 2018 Citations: 119
DRD4 and DAT1 as modifying genes in alcoholism: interaction with novelty seeking on level of alcohol consumption CHD Bau, S Almeida, FT Costa, CED Garcia, EP Elias, AC Ponso, ... Molecular Psychiatry 6 (1), 7-9 , 2001 2001 Citations: 78
Estrogen receptor 2 and progesterone receptor gene polymorphisms and lipid levels in women with different hormonal status S Almeida, N Franken, MR Zandona, MC Osorio-Wender, MH Hutz The pharmacogenomics journal 5 (1), 30-34 , 2005 2005 Citations: 64
Oxytocin modulates social interaction but is not essential for sexual behavior in male mice VM Lazzari, RO Becker, MS de Azevedo, M Morris, K Rigatto, S Almeida, ... Behavioural brain research 244, 130-136 , 2013 2013 Citations: 62
Dopamine D4 receptor gene and personality dimensions in Brazilian male alcoholics. CH Bau, T Roman, S Almeida, MH Hutz Psychiatric Genetics 9 (3), 139-143 , 1999 1999 Citations: 62
Association of a serotonin transporter gene polymorphism (5-HTTLPR) and stressful life events with postpartum depressive symptoms: a population-based study R Tavares Pinheiro, F Monteiro da Cunha Coelho, R Azevedo da Silva, ... Journal of Psychosomatic Obstetrics & Gynecology 34 (1), 29-33 , 2013 2013 Citations: 51
Association between a frequent variant of the FTO gene and anthropometric phenotypes in Brazilian children CF da Silva, MR Zandoná, MR Vitolo, PDB Campagnolo, LN Rotta, ... BMC medical genetics 14 (1), 34 , 2013 2013 Citations: 50
Association of MAOA and COMT gene polymorphisms with palatable food intake in children ACS Galvão, RC Krüger, PDB Campagnolo, VS Mattevi, MR Vitolo, ... The Journal of nutritional biochemistry 23 (3), 272-277 , 2012 2012 Citations: 49
Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children SV Melo, G Agnes, MR Vitolo, VS Mattevi, PDB Campagnolo, S Almeida Genetics and molecular biology 40 (2), 415-420 , 2017 2017 Citations: 45
Estrogen receptor 1 gene polymorphisms and coronary artery disease in the Brazilian population S Almeida, MH Hutz Brazilian journal of medical and biological research 39 (4), 447-454 , 2006 2006 Citations: 39
Hepatocellular carcinoma and estrogen receptors: Polymorphisms and isoforms relations and implications VD Baldissera, AF Alves, S Almeida, M Porawski, M Giovenardi Medical hypotheses 86, 67-70 , 2016 2016 Citations: 35
Validation of obesity susceptibility loci identified by genome‐wide association studies in early childhood in South Brazilian children MR Zandoná, CN Sangalli, PB Campagnolo, MR Vitolo, S Almeida, ... Pediatric obesity 12 (1), 85-92 , 2017 2017 Citations: 31
ESR1 and APOE gene polymorphisms, serum lipids, and hormonal replacement therapy S Almeida, M Fiegenbaum, FM de Andrade, MC Osório-Wender, MH Hutz Maturitas 54 (2), 119-126 , 2006 2006 Citations: 31
Lack of association of the dopamine D4 receptor gene polymorphism with alcoholism in a Brazilian population T Roman, CHD Bau, S Almeida, MH Hutz Addiction biology 4 (2), 203-207 , 1999 1999 Citations: 31
Analysis of transcriptional levels of the oxytocin receptor in different areas of the central nervous system and behaviors in high and low licking rats CA Ruthschilling, G Albiero, VM Lazzari, RO Becker, AC de Moura, ... Behavioural brain research 228 (1), 176-184 , 2012 2012 Citations: 30
Gene expression in the CNS of lactating rats with different patterns of maternal behavior AC de Moura, VM Lazzari, RO Becker, MS Gil, CA Ruthschilling, G Agnes, ... Neuroscience research 99, 8-15 , 2015 2015 Citations: 29
DRD4 and SLC6A3 gene polymorphisms are associated with food intake and nutritional status in children in early stages of development C Fontana, MR Vitolo, PDB Campagnolo, VS Mattevi, JP Genro, ... The Journal of nutritional biochemistry 26 (12), 1607-1612 , 2015 2015 Citations: 28
The lactase persistence genotype is a protective factor for the metabolic syndrome DC Friedrich, FM de Andrade, M Fiegenbaum, S Almeida, VS Mattevi, ... Genetics and molecular biology 37 (4), 611-615 , 2014 2014 Citations: 28
The impact of oxytocin gene knockout on sexual behavior and gene expression related to neuroendocrine systems in the brain of female mice JM Zimmermann-Peruzatto, VM Lazzari, G Agnes, RO Becker, ... Cellular and molecular neurobiology 37 (5), 803-815 , 2017 2017 Citations: 27