Kayode Muiritala Salawu
@unilorin.edu.ng
Lecturer, Faculty of Pharmaceutical Sciences
University of Ilorin
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmacy, Cancer Research, Drug Discovery, Chemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Kayode Muritala Salawu, Omonike Oluyemisi Ogbole, Oyindamola Oduola Abiodun, and Yan Wang
Bentham Science Publishers Ltd.
Introduction: Globally, about 8.2 million cancer-related deaths are recorded annually. Sadly, most of the deaths result from the toxicity of most chemotherapeutic agents. Hence, there are growing demands for chemotherapeutic agents with high specificity and selectivity. This study was designed to assess the cytotoxic potential of Detarium microcarpum and isolate cytotoxic compounds with better selectivity profiles. Methods: Detarium microcarpum Stem bark (DMS) was collected and authenticated at the Forest Herbarium Ibadan (FHI), and a voucher (FHI-111954) was issued. Dried DMS was pulverized and extracted into 70% methanol. The extract was partitioned into hexane, dichloromethane, and ethyl acetate fractions. The cytotoxicities of the extract, fractions, and isolated compounds were determined. The cytotoxicity of the isolated compounds was tested against different cell lines, including human breast (AU565 and MDA MB231), oral adenosquamous (CAL27), and cervical (HeLa) cancer cells, as well as healthy (3T3) non-cancer cells. Results: Methyl gallate, eriodictyol, quercetin, quebrachitol, catechin, catechin gallate, and gallic acid, isolated from dichloromethane and ethyl acetate fractions, displayed weak cytotoxicity against breast (AU565 and MDAMD- 231) and cervical (HeLa) cancer cell lines. Interestingly, all the compounds, except gallic acid (48.91±4.51% inhibition), displayed potent cytotoxicity on oral cancer cells. Methyl gallate and quercetin displayed the highest activity, with IC50 values of 89.57±1.98μM and 78.19±1.49μM, respectively. Interestingly, all the compounds were not toxic to healthy non-cancer (3T3) cells. Conclusion: The compounds displayed anticancer activity specific to oral cancer cells and were highly selective for cancer cells without causing significant toxicity to healthy non-cancer cells.
Raza Ali, Kayode Muritala Salawu, Muhammad Aamer, Humera Jahan, Priya Tufail, Rimsha Irshad, Farooq-Ahmad Khan, Bilge Sener, M. Iqbal Choudhary, and Yan Wang
Informa UK Limited
A new sesquiterpene (Prosoterpene, 1) and eleven reported compounds (2-12) of several classes, such as flavonoids, alkaloids, phenolic acids, and long-chain alcohols, were isolated from the BuOH extract of Prosopis africana (Guill. & Perr.) Taub. Compounds 2-10 were reported for the first time from this plant. Isomers 11 and 12 were separated for the first time. Extensive spectroscopic techniques and literature comparisons were used to characterise their structures. Furthermore, compounds 3, 5-8, and 10-12 were performed for anti-glycation and cytotoxicity activities. Compound 3 (quercetin-3-O-α-L-rhamnoside) exhibited moderate anti-glycation activity. All tested compounds were non-cytotoxic against MCF-7 (breast cancer), NCI-H460 (lung cancer), Hela (cervical cancer), and BJ (normal human fibroblast) cell lines.
Rimsha Irshad, Ahmed Saeed Ali Kabbashi, Kayode Muritala Salawu, Aziz ur-Rehman, Yan-Gang Cao, Aneela Fayaz, Farooq-Ahmad Khan, Atia tul-Wahab, M. Iqbal Choudhary, and Yan Wang
Elsevier BV
Nida Ali, Farooq-Ahmad Khan, Kayode Muritala Salawu, Rimsha Irshad, Almas Jabeen, Chun-Lei Zhang, Muhammad Iqbal Choudhary, Xin-Min Liu, and Yan Wang
MDPI AG
Two new ursane-type triterpenoids, named Polyanside A (1) and B (2), along with eleven known compounds (3–13), were isolated and elucidated from Maranthes polyandra (Benth.) Prance. The structures of these compounds were elucidated based on chemical evidence and multiple spectroscopic data. Isolated compounds were evaluated for anti-cancer, anti-inflammatory activities, and cytotoxicity on a normal human cell line (BJ). None of them showed activity and cytotoxicity. The hexane fraction was analyzed by GC-MS, resulting in the identification of forty-one compounds. This is the first comprehensive study on the phytochemistry of M. polyandra.
Olalere Shittu, Olufunke Adenike Opeyemi, Olumuyiwa Babagbemi Omotesho, Oluwatosin Fakayode, Nnaemeka Asogwa, Opeyemi Margaret Adeniyi, Ifeoluwa Margaret Fatoba, Kayode Muritala Salawu, Olusola Ajibaye, Olarewaju Abdulkareem Babamale,et al.
Charles University in Prague, Karolinum Press
Background: A tremendous level of success has been achieved since the introduction of chloroquine and the combination of amodiaquine and artemisinin for the treatment of both complicated and uncomplicated malaria infections in sub-Saharan Africa. However, the recent discovery of drug resistant strains of Plasmodium falciparum (P.f.) and the ability of the parasite to ingest CYP2C8 into its digestive vacuole is of great public health concern. This study probes the occurrence of CYP2C8*2 allelic mutant amongst malaria patients in North-Central Nigeria. Methods: Three hundred and eighty five (385) unrelated study participants were screened for current malaria episodes using routine microscopy and/or rapid diagnostic test strips (RDTs). Chelex extraction method was used for single nucleotide polymorphisms (SNPs) and identification of CYP2C8*2 (805A > T) variant respectively. Wild-type (A) and the defective allele (T) were differentiated with the use of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The results obtained were further validated with Sanger sequencing of a few samples and thereafter, the genotype data were statistically processed. All alleles obtained were in Hardy Weinberg equilibrium. Results: Out of the 385 participants (45.5% Male and 54.5% Female) genotyped for SNPs, 75 (19.5%) had the autosomal recessive mutant trait. Occurrence of mutant traits was gender and ethnic independent (p > 0.05). Yoruba ethnic group recorded a reduction in proportion of genotypic defective CYP2C8*2 allele (T) (1 in every 8 persons) with a carrier percentage of 13.3% compared with Hausa (26.62%); Igbo (25.37%) and other minority ethnic groups (17.6%). Conclusions: A remarkable inter-ethnic differences in autosomal recessive CYP2C8*2 allele was observed. By implication, there is a gradual incursion of genetic drift for poor CQ and AQ-Artemisinin metabolizers among the inhabitants.
Kayode Muritala Salawu, Edith Oriabure Ajaiyeoba, Omonike Oluyemisi Ogbole, Johnson Adekunle Adeniji, Temitope Cephas Faleye, and Abdulkarim Agunu
Informa UK Limited
ABSTRACT Leaf and gel extracts of Aloe schweinfurthii and A. vera were subjected to in vitro antioxidant assay using 2,2-diphennyl-1-picryl hydrazyl (DPPH), brine shrimp lethality bioassay, and cytotoxicity using the MTT assay with two human cancer cell lines: Rd and Hep-2c. Extracts of A. schweinfurthii gel had IC50 values of 44.59; A. vera gel, 41.48, and A. vera leaf, 38.84 µg.mL–1, had similar DPPH radical-scavenging properties and were more active than A. schweinfurthii leaf. Ascorbic acid had an IC50 of 9.26 ± 0.14 µg.mL–1. Aloe vera leaf (LC50 = 325 ± 5.38 µg.mL–1) was more active than the other three extracts in the BSL assay. The gel extracts of A. schweinfurthii (CC50 = 4.06 µg.mL–1on Rd and 9.00 µg.mL–1 on Hep-2c) and A. vera (CC50 = 4.31 µg.mL–1 on Rd, 9.06 µg.mL–1 on Hep-2c) elicited similar and more potent cytotoxicity comparable to cyclophosphamide, with CC50 = 2.2 µg.mL–1 on Rd and 2.66 µg.mL–1 on Hep-2c. Leaf extracts were less active than gel extracts. This study showed that A. schweinfurthii gel, A. vera gel and leaf had weak DPPH activity which were similar. Aloe vera and A. schweinfurthii did not elicit potent cytotoxicity in BSL assay. The gels from both Aloe species displayed antipoliferative activity on Rd and Hep-2c, two human cell cancer lines.